Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,124	+G	intergenic (+58/+26)	ilvC → / ← ppiC	ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,123	1	.	G	99.3%	1248.2 / 0.2	406	intergenic (+57/+27)	ilvC/ppiC	ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (116/286); total (118/288)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 454 total aligned reads displayed.

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑C‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC  >  NC_000913/3957033‑3957210

gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCGCCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑aa                                                                                           <  1:1235996/93‑1 (MQ=21)

gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑aa                                                                                           <  1:898495/93‑1 (MQ=21)

gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑aa                                                                                           <  1:8012441/93‑1 (MQ=21)

    gATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑ggcc                                                                                      <  2:2938767/93‑4 (MQ=21)

     atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑ggcct                                                                                     <  2:3988260/93‑5 (MQ=21)

       atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTtc                                                                                   <  2:3481177/93‑1 (MQ=18)

       atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTtc                                                                                   <  1:2255090/93‑1 (MQ=18)

       atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTtc                                                                                   <  2:7101305/93‑1 (MQ=18)

       atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTtc                                                                                   <  1:7571164/93‑1 (MQ=18)

        tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTtct                                                                                  <  2:2438971/93‑1 (MQ=21)

         gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTtctc                                                                                 <  1:7492/93‑1 (MQ=18)

          aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTcc                                                                                <  2:3819367/93‑1 (MQ=18)

           aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCt                                                                               <  2:861986/93‑1 (MQ=18)

           aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTACCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCt                                                                               <  1:3372674/93‑1 (MQ=18)

              gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGga                                                                            <  1:7239353/93‑1 (MQ=18)

               tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑CCTCCCCCCGGGAGAGGGGGAAAACACTC‑AAGG‑CCTTCTCCTGgag                                                                           >  1:5963737/1‑93 (MQ=18)

                 ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGTCC‑‑C‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGaa                                                                         <  2:4941495/93‑1 (MQ=18)

                 ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGaa                                                                         <  2:3204078/93‑1 (MQ=18)

                  tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAg                                                                        <  1:2788448/93‑1 (MQ=18)

                  tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAg                                                                        <  1:6410895/93‑1 (MQ=18)

                   gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGTCC‑‑C‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAgg                                                                       <  1:4434307/93‑1 (MQ=18)

                   gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAgg                                                                       <  1:2617012/93‑1 (MQ=18)

                    cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCC‑‑ACCC‑‑TCTCCCACAGGGAGAGGGGGAAAACACTCAAA‑G‑CCTTCTCCTCGAGAAGGc                                                                      >  1:1466417/1‑93 (MQ=18)

                     cgtcgCGGGTTGAGCGCGCGCTGGTGCCCTCCCCCGG‑‑A‑‑CGGTCGCCCCCAGGGAGAGGGAGAAAACTCTC‑AAGG‑CGGTCTCCGGGAGAAGGcc                                                                     <  2:1261663/89‑1 (MQ=9)

                       ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCtt                                                                   <  2:7480980/93‑1 (MQ=18)

                        tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTg                                                                  <  1:6256511/93‑1 (MQ=18)

                        tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTg                                                                  <  2:4405447/93‑1 (MQ=18)

                         gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGc                                                                 <  2:193179/93‑1 (MQ=18)

                              ttAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑C‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTa                                                            <  1:3089720/93‑1 (MQ=18)

                              ttAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTa                                                            <  2:8367788/93‑1 (MQ=18)

                              ttAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTa                                                            <  1:2486596/93‑1 (MQ=18)

                              ttAAGTGCGCGCTGATGCCCTCAACCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTa                                                            <  2:4826346/93‑1 (MQ=18)

                               tAAGTGCGCGCTGATCCCCTCACCCCGTCC‑‑C‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAg                                                           <  2:6180829/93‑1 (MQ=18)

                                aaGTGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGt                                                          <  2:8775408/93‑1 (MQ=18)

                                  gTGCGCGCTGATGCCCTCACCCCGCCC‑‑C‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                        <  2:1638164/93‑1 (MQ=18)

                                   tGCGCGCTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                       <  2:4798599/93‑1 (MQ=21)

                                    gcgcgcTGATGCCCTCCCCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                      <  1:4062273/93‑1 (MQ=21)

                                     cgcgcTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                     <  1:5919863/93‑1 (MQ=21)

                                     cgcgcTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                     <  1:3721547/93‑1 (MQ=21)

                                        gcTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                  <  2:4588967/93‑1 (MQ=21)

                                         cTGATGCCCTCCCCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                 <  2:577530/93‑1 (MQ=21)

                                         cTGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                 <  1:689705/93‑1 (MQ=21)

                                          tGATGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg                                                <  1:5181920/93‑1 (MQ=25)

                                           gATGCCCTCCCCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                               <  2:2209580/93‑1 (MQ=25)

                                            aTGCCCTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCa                                              <  2:4134697/93‑1 (MQ=25)

                                               cccTCACCCCGCCC‑‑C‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt                                           <  2:6533556/93‑1 (MQ=25)

                                                ccTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCtt                                          <  1:6729982/93‑1 (MQ=25)

                                                 cTCACCCCG‑‑ACCC‑‑TCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTa                                         >  1:885481/1‑93 (MQ=37)

                                                 cTCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTa                                         <  2:5321132/93‑1 (MQ=37)

                                                  tCACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                        >  1:8613684/1‑93 (MQ=37)

                                                   cACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                       <  2:6702259/93‑1 (MQ=37)

                                                   cACCCCG‑‑ACCC‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                       >  2:4599892/1‑93 (MQ=37)

                                                           gaccc‑‑tctCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                 >  1:4300120/5‑93 (MQ=38)

                                                           gaccc‑‑tctCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                 <  1:4662021/89‑1 (MQ=38)

                                                            accc‑‑tctCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                >  1:1847221/4‑93 (MQ=255)

                                                            accc‑‑tctCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                >  2:7902350/4‑93 (MQ=255)

                                                             ccc‑‑tctCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat                               >  1:7114986/3‑93 (MQ=255)

                                                               c‑‑tctCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGatat                             <  1:5588017/93‑1 (MQ=255)

                                                               c‑‑tctCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGatat                             <  2:2531616/93‑1 (MQ=255)

                                                                  tctcCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                            <  2:1194602/93‑1 (MQ=255)

                                                                  tctcCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                            <  1:812676/93‑1 (MQ=255)

                                                                   ctcCCACAGGGAGATGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                           <  1:1149027/93‑1 (MQ=255)

                                                                   ctcCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                           >  2:1347502/1‑93 (MQ=255)

                                                                   ctcCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                           <  1:4928886/93‑1 (MQ=255)

                                                                   ctcCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                           <  2:7623335/93‑1 (MQ=255)

                                                                    tcCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg                          >  1:7682443/1‑93 (MQ=255)

                                                                      ccACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa                        >  1:737224/1‑93 (MQ=255)

                                                                      ccACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa                        >  1:2070981/1‑93 (MQ=255)

                                                                      ccACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGAACAGCACCTTAATGATGTGATATCCGaa                        >  1:2842497/1‑93 (MQ=255)

                                                                       cacaGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAc                       <  1:842421/93‑1 (MQ=255)

                                                                        acaGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACt                      <  2:1711625/93‑1 (MQ=255)

                                                                         caGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                     >  2:3356684/1‑93 (MQ=255)

                                                                         caGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                     >  1:8275275/1‑93 (MQ=255)

                                                                          agggagagggagAAAACACTC‑AATG‑CCTTCTCCTGGAGAAGGCCTTTCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                    <  1:328640/93‑1 (MQ=255)

                                                                            ggagagggagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                  <  2:3662262/93‑1 (MQ=255)

                                                                             gagagggagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACGGCACCTTACTGATGTGATATCCGAACTGGgtg                 >  1:4575132/1‑93 (MQ=255)

                                                                              agagggagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                <  1:57063/93‑1 (MQ=255)

                                                                                agggagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc              >  1:4798731/1‑93 (MQ=255)

                                                                                aggaagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc              <  2:7799863/93‑1 (MQ=255)

                                                                                 gggagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa             <  2:8247093/93‑1 (MQ=255)

                                                                                 gggagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa             >  2:7373894/1‑93 (MQ=255)

                                                                                 gggagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa             <  2:5018840/93‑1 (MQ=255)

                                                                                  gatgaAAACACTC‑AA‑GTCCTTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAg            <  1:4165750/90‑1 (MQ=255)

                                                                                   gagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc           <  2:700450/93‑1 (MQ=255)

                                                                                   gagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc           >  2:2056174/1‑93 (MQ=255)

                                                                                   gagaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc           <  2:2769309/93‑1 (MQ=255)

                                                                                    agaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg          <  1:6630693/93‑1 (MQ=255)

                                                                                     gaAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGAGTGCAGCgg         >  2:5452531/1‑93 (MQ=255)

                                                                                        aaCACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc      <  1:6073628/93‑1 (MQ=255)

                                                                                        aaCACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc      >  1:6214593/1‑93 (MQ=255)

                                                                                          cacTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCgg    <  1:5422970/93‑1 (MQ=255)

                                                                                           ccTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  1:7876075/92‑1 (MQ=255)

                                                                                           acTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  2:1412593/93‑1 (MQ=255)

                                                                                           acTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  1:1651003/93‑1 (MQ=255)

                                                                                           acTC‑AAGG‑CCTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt   <  2:1818352/93‑1 (MQ=255)

                                                                                            cTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGcggtccggtc  <  2:7985831/93‑1 (MQ=255)

                                                                                            cTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  <  1:3826606/93‑1 (MQ=255)

                                                                                            cTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  <  2:5973124/93‑1 (MQ=255)

                                                                                            cTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  <  2:7295612/93‑1 (MQ=255)

                                                                                            cTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc  <  1:1019044/93‑1 (MQ=255)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑C‑‑TCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC  >  NC_000913/3957033‑3957210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 275 total aligned reads displayed.

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  >  NC_000913/3957033‑3957217

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                                <  SRR4302541.5324136/94‑1 (MQ=60)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                                <  SRR4302541.906803/94‑1 (MQ=60)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                                <  SRR4302541.902297/94‑1 (MQ=60)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G                                                                                                <  SRR4302541.8100555/94‑1 (MQ=60)

 ACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G‑G                                                                                              <  SRR4302541.6730762/94‑1 (MQ=60)

 ACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G‑G                                                                                              <  SRR4302541.4728919/94‑1 (MQ=60)

  CAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G‑GC                                                                                             <  SRR4302541.6024087/94‑1 (MQ=60)

     ATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTT                                                                                           <  SRR4302541.1004747/94‑1 (MQ=60)

     ATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTT                                                                                           <  SRR4302541.2988182/94‑1 (MQ=60)

       ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCT                                                                                         <  SRR4302541.2276866/94‑1 (MQ=60)

       ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCT                                                                                         <  SRR4302541.5508203/94‑1 (MQ=60)

       ATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCT                                                                                         <  SRR4302541.4920552/94‑1 (MQ=60)

        TGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTC                                                                                        <  SRR4302541.2895246/94‑1 (MQ=60)

         GAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCC                                                                                       <  SRR4302541.2070582/94‑1 (MQ=60)

         GAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCC                                                                                       <  SRR4302541.7544/94‑1 (MQ=60)

         GAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCC                                                                                       <  SRR4302541.6453792/94‑1 (MQ=60)

          AAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCT                                                                                      <  SRR4302541.8700810/94‑1 (MQ=60)

          AAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCT                                                                                      <  SRR4302541.120438/94‑1 (MQ=60)

          AAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCT                                                                                      <  SRR4302541.7686941/94‑1 (MQ=60)

          AAACGTATGGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCGCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCT                                                                                      <  SRR4302541.899251/94‑1 (MQ=47)

           AACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTG                                                                                     <  SRR4302541.1566831/94‑1 (MQ=60)

           AACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCACCCACCGGGCGAGGGGGAAAACAATC‑AAGG‑ACTTCTCCTG                                                                                     >  SRR4302541.2749479/1‑94 (MQ=37)

            ACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGG                                                                                    <  SRR4302541.7763157/94‑1 (MQ=60)

              GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAG                                                                                  <  SRR4302541.7998201/94‑1 (MQ=60)

              GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAG                                                                                  <  SRR4302541.7316558/94‑1 (MQ=60)

              GTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCCCTCCCCCCGGGAGAGGGGGAAAACACTC‑AAGG‑CCTTCTCCTGGAG                                                                                  >  SRR4302541.6024523/1‑94 (MQ=52)

               TATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGA                                                                                 <  SRR4302541.6061759/94‑1 (MQ=60)

                ATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAA                                                                                <  SRR4302541.5652214/94‑1 (MQ=60)

                 TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAG                                                                               <  SRR4302541.8862739/94‑1 (MQ=60)

                 TTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAG                                                                               <  SRR4302541.6085729/94‑1 (MQ=60)

                  TGCTGTTGCGGGTTAGTTGCGCGCAGATCCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACCCTC‑AAGG‑CCTTGTCCTGGAGAAGG                                                                              <  SRR4302541.6476277/94‑1 (MQ=13)

                  TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGG                                                                              <  SRR4302541.6476279/94‑1 (MQ=60)

                  TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGG                                                                              <  SRR4302541.1185358/94‑1 (MQ=60)

                  TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGG                                                                              <  SRR4302541.2816585/94‑1 (MQ=60)

                   GCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGTCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGC                                                                             <  SRR4302541.4478876/94‑1 (MQ=49)

                   GCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGC                                                                             <  SRR4302541.7617963/94‑1 (MQ=60)

                   GCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCC‑‑ACCCTCTCCCACAGGGAGAGGGGGAAAACACTCAAA‑G‑CCTTCTCCTCGAGAAGGC                                                                             >  SRR4302541.1480779/1‑94 (MQ=45)

                     TGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCT                                                                           <  SRR4302541.7537097/94‑1 (MQ=60)

                     TGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCT                                                                           <  SRR4302541.6930083/94‑1 (MQ=60)

                      GTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTT                                                                          <  SRR4302541.7640995/94‑1 (MQ=60)

                       TTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTG                                                                         <  SRR4302541.2325009/94‑1 (MQ=60)

                       TTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTG                                                                         <  SRR4302541.7010772/94‑1 (MQ=60)

                        TGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGC                                                                        <  SRR4302541.6320419/94‑1 (MQ=60)

                          CGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CCCTCCCACAGGGAGAGGGAGGAAAAACTC‑AACG‑CCTTCTCCTGGAGAAGGCCCTGCTT                                                                      >  SRR4302541.5904319/1‑94 (MQ=21)

                           gcggggtaaggtccccccggatcccctccccccaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCT                                                                       <  SRR4302541.4400611/58‑1 (MQ=60)

                           GGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTAT                                                                     <  SRR4302541.5784832/94‑1 (MQ=60)

                             GTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTA                                                                   <  SRR4302541.6625119/94‑1 (MQ=60)

                              TTAAGTGCGCGCTGATGCCCTCACCCCGCCC‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAG                                                                  <  SRR4302541.3121057/94‑1 (MQ=49)

                                AAGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTT                                                                <  SRR4302541.6254275/94‑1 (MQ=60)

                                 AGTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTG                                                               <  SRR4302541.6393269/94‑1 (MQ=60)

                                  GTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGC                                                              <  SRR4302541.2734736/94‑1 (MQ=60)

                                  GTGCGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGC                                                              <  SRR4302541.2503555/94‑1 (MQ=60)

                                    GCGCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGG                                                            <  SRR4302541.4102366/94‑1 (MQ=60)

                                     CGCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGT                                                           <  SRR4302541.5980132/94‑1 (MQ=60)

                                      GCGCTGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTA                                                          <  SRR4302541.6833158/94‑1 (MQ=60)

                                      GCGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTA                                                          <  SRR4302541.1692941/94‑1 (MQ=60)

                                       CGCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAC                                                         <  SRR4302541.3719733/94‑1 (MQ=60)

                                        GCTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACA                                                        <  SRR4302541.7689973/94‑1 (MQ=60)

                                         CTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAG                                                       <  SRR4302541.2864394/94‑1 (MQ=60)

                                         CTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAG                                                       <  SRR4302541.4220394/94‑1 (MQ=60)

                                         CTGATGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAG                                                       <  SRR4302541.696199/94‑1 (MQ=60)

                                          TGATGCCCTCCCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGC                                                      <  SRR4302541.6312317/94‑1 (MQ=60)

                                             TGCCCTCGCCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACC                                                   <  SRR4302541.6312877/94‑1 (MQ=60)

                                             TGCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACC                                                   <  SRR4302541.3816771/94‑1 (MQ=60)

                                              GCCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCT                                                  <  SRR4302541.5062429/94‑1 (MQ=60)

                                               CCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTT                                                 <  SRR4302541.7336756/94‑1 (MQ=60)

                                               CCCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTT                                                 <  SRR4302541.1235232/94‑1 (MQ=60)

                                                CCTCACCCCG‑‑ACCCTCTCCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTA                                                >  SRR4302541.893588/1‑94 (MQ=60)

                                                CCTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTA                                                <  SRR4302541.6801813/94‑1 (MQ=60)

                                                 CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGTAACTTAA                                               >  SRR4302541.4253689/1‑94 (MQ=60)

                                                 CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                               >  SRR4302541.8706049/1‑94 (MQ=60)

                                                 CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                               >  SRR4302541.5364847/1‑94 (MQ=60)

                                                 CTCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAA                                               <  SRR4302541.6731197/94‑1 (MQ=60)

                                                  TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTGTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT                                              >  SRR4302541.4544586/1‑94 (MQ=60)

                                                  TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT                                              <  SRR4302541.7741799/94‑1 (MQ=60)

                                                  TCACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT                                              <  SRR4302541.7453328/94‑1 (MQ=60)

                                                   CACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACAATC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATG                                             >  SRR4302541.2000348/1‑94 (MQ=60)

                                                    ccctcccgcaccccgCCCACAGGGAGAGCGCGAAAACACTC‑AAGG‑CCTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAT                                              <  SRR4302541.6714822/79‑1 (MQ=60)

                                                    ACCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                            >  SRR4302541.6167682/1‑94 (MQ=60)

                                                     CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTActgct                                           >  SRR4302541.4597086/1‑89 (MQ=60)

                                                     CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                           >  SRR4302541.6659317/1‑94 (MQ=60)

                                                     CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                           <  SRR4302541.4764290/94‑1 (MQ=60)

                                                     CCCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAT                                           >  SRR4302541.4597087/1‑94 (MQ=60)

                                                      cccccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CTTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                            <  SRR4302541.2853937/85‑1 (MQ=60)

                                                      cccccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                            <  SRR4302541.2109238/85‑1 (MQ=60)

                                                      cccccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                            <  SRR4302541.360408/85‑1 (MQ=60)

                                                      cccccgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGA                                            <  SRR4302541.8448566/85‑1 (MQ=60)

                                                      CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG                                          >  SRR4302541.7847217/1‑94 (MQ=60)

                                                      CCCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATG                                          >  SRR4302541.8856836/1‑94 (MQ=60)

                                                       CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT                                         <  SRR4302541.5738462/94‑1 (MQ=60)

                                                       CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT                                         >  SRR4302541.2331216/1‑94 (MQ=60)

                                                       CCG‑‑ACCCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGT                                         >  SRR4302541.1151706/1‑94 (MQ=60)

                                                          cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG                                        >  SRR4302541.4343062/6‑94 (MQ=60)

                                                          cgaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTG                                        >  SRR4302541.6795447/6‑94 (MQ=60)

                                                           gaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA                                       >  SRR4302541.1727015/5‑94 (MQ=60)

                                                           gaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA                                       >  SRR4302541.2470044/5‑94 (MQ=60)

                                                           gaccCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGA                                       <  SRR4302541.4709492/90‑1 (MQ=60)

                                                            accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                      <  SRR4302541.3027814/91‑1 (MQ=60)

                                                            accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                      >  SRR4302541.3199953/4‑94 (MQ=60)

                                                            accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                      >  SRR4302541.7190769/4‑94 (MQ=60)

                                                            accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                      >  SRR4302541.3358031/4‑94 (MQ=60)

                                                            accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                      >  SRR4302541.5144739/4‑94 (MQ=60)

                                                            accCTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAT                                      <  SRR4302541.6247723/91‑1 (MQ=60)

                                                            C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATAT                                    <  SRR4302541.4871365/94‑1 (MQ=60)

                                                            C‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGCTACAGCACCTTAATGATGTGATAT                                    <  SRR4302541.2143925/94‑1 (MQ=60)

                                                               CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC                                   <  SRR4302541.5645218/94‑1 (MQ=60)

                                                               CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC                                   <  SRR4302541.3637781/94‑1 (MQ=60)

                                                               CTCGCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATC                                   <  SRR4302541.5076625/94‑1 (MQ=60)

                                                                TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCC                                  <  SRR4302541.924646/94‑1 (MQ=60)

                                                                TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCC                                  <  SRR4302541.4779852/94‑1 (MQ=60)

                                                                TCTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCC                                  <  SRR4302541.436579/94‑1 (MQ=60)

                                                                 CTCCCACAGGGAGATGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCG                                 <  SRR4302541.1159981/94‑1 (MQ=60)

                                                                 CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCG                                 >  SRR4302541.6006362/1‑94 (MQ=60)

                                                                 CTCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCG                                 <  SRR4302541.4979181/94‑1 (MQ=60)

                                                                  TCCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGA                                >  SRR4302541.3302790/1‑94 (MQ=60)

                                                                  TCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGA                                <  SRR4302541.7215246/94‑1 (MQ=60)

                                                                  TCCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGA                                <  SRR4302541.8377644/94‑1 (MQ=60)

                                                                   CCCACGGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAA                               <  SRR4302541.1991630/94‑1 (MQ=60)

                                                                   CCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAA                               >  SRR4302541.4656235/1‑94 (MQ=60)

                                                                   CCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAA                               >  SRR4302541.744235/1‑94 (MQ=60)

                                                                   CCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAA                               >  SRR4302541.2090997/1‑94 (MQ=60)

                                                                   CCCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGAACAGCACCTTAATGATGTGATATCCGAA                               >  SRR4302541.2871077/1‑94 (MQ=60)

                                                                    CCACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAC                              >  SRR4302541.2576981/1‑94 (MQ=60)

                                                                     CACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACT                             <  SRR4302541.7817542/94‑1 (MQ=60)

                                                                     CACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACT                             <  SRR4302541.4087505/94‑1 (MQ=60)

                                                                     CACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACT                             <  SRR4302541.332711/94‑1 (MQ=60)

                                                                     CACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACT                             >  SRR4302541.8183458/1‑94 (MQ=60)

                                                                     CACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACT                             <  SRR4302541.850064/94‑1 (MQ=60)

                                                                      ACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTG                            >  SRR4302541.8200111/1‑94 (MQ=60)

                                                                      ACAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTG                            >  SRR4302541.8365318/1‑94 (MQ=60)

                                                                       CAGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGG                           <  SRR4302541.1876521/94‑1 (MQ=60)

                                                                        AGGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGG                          <  SRR4302541.331587/94‑1 (MQ=60)

                                                                         GGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGT                         <  SRR4302541.1402541/94‑1 (MQ=60)

                                                                         GGGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGAGGTACAGCACCTTAATGATGTGATATCCGAACTGGGT                         <  SRR4302541.5568668/94‑1 (MQ=60)

                                                                          GGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACGGCACCTTACTGATGTGATATCCGAACTGGGTG                        >  SRR4302541.4621477/1‑94 (MQ=60)

                                                                          GGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTG                        <  SRR4302541.79976/94‑1 (MQ=60)

                                                                          GGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTG                        <  SRR4302541.4040270/94‑1 (MQ=60)

                                                                          GGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTG                        <  SRR4302541.6413618/94‑1 (MQ=60)

                                                                          GGAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTG                        >  SRR4302541.4621478/1‑94 (MQ=60)

                                                                           GAGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGT                       <  SRR4302541.1601087/94‑1 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                      <  SRR4302541.3293152/94‑1 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                      >  SRR4302541.808299/1‑94 (MQ=60)

                                                                            AGAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTG                      <  SRR4302541.57515/94‑1 (MQ=60)

                                                                             GAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGC                     >  SRR4302541.4161651/1‑94 (MQ=60)

                                                                             GAGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGC                     >  SRR4302541.4847677/1‑94 (MQ=60)

                                                                              AGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCA                    >  SRR4302541.4474563/1‑94 (MQ=60)

                                                                              AGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCA                    <  SRR4302541.7645981/94‑1 (MQ=60)

                                                                              AGGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCA                    >  SRR4302541.8318826/1‑94 (MQ=60)

                                                                               GGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAG                   <  SRR4302541.7842335/94‑1 (MQ=60)

                                                                               GGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAG                   <  SRR4302541.7391221/94‑1 (MQ=60)

                                                                               GGGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAG                   <  SRR4302541.2527102/94‑1 (MQ=60)

                                                                                gatGAAAACACTC‑AA‑GTCCTTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGC                  <  SRR4302541.4207000/91‑1 (MQ=60)

                                                                                GGAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGC                  <  SRR4302541.4206999/94‑1 (MQ=60)

                                                                                 GAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCG                 <  SRR4302541.5653373/94‑1 (MQ=60)

                                                                                 GAGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCG                 <  SRR4302541.1385767/94‑1 (MQ=60)

                                                                                  AGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTCTTAGCTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG                >  SRR4302541.1641726/1‑94 (MQ=60)

                                                                                  AGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG                >  SRR4302541.7306035/1‑94 (MQ=60)

                                                                                  AGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG                <  SRR4302541.5345885/94‑1 (MQ=60)

                                                                                  AGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG                <  SRR4302541.281358/94‑1 (MQ=60)

                                                                                  AGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG                <  SRR4302541.2613505/94‑1 (MQ=60)

                                                                                  AGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG                <  SRR4302541.3879115/94‑1 (MQ=60)

                                                                                  AGAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGG                >  SRR4302541.3779933/1‑94 (MQ=60)

                                                                                   GAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG               >  SRR4302541.110618/1‑94 (MQ=60)

                                                                                   GAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG               >  SRR4302541.3752113/1‑94 (MQ=60)

                                                                                   GAAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGG               >  SRR4302541.3774332/1‑94 (MQ=60)

                                                                                    AAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGC              >  SRR4302541.4710885/1‑94 (MQ=60)

                                                                                    AAAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGC              >  SRR4302541.8233773/1‑94 (MQ=60)

                                                                                     AAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCC             >  SRR4302541.5618178/1‑94 (MQ=60)

                                                                                     AAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCC             <  SRR4302541.6164456/94‑1 (MQ=60)

                                                                                     AAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCC             >  SRR4302541.8220067/1‑94 (MQ=60)

                                                                                     AAACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCC             >  SRR4302541.6278102/1‑94 (MQ=60)

                                                                                      AACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCG            >  SRR4302541.51941/1‑94 (MQ=60)

                                                                                       ACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG           <  SRR4302541.2163905/94‑1 (MQ=60)

                                                                                       ACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG           <  SRR4302541.2674498/94‑1 (MQ=60)

                                                                                       ACACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGG           <  SRR4302541.703999/94‑1 (MQ=60)

                                                                                        CACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGT          <  SRR4302541.5478613/94‑1 (MQ=60)

                                                                                        CACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGT          >  SRR4302541.8546951/1‑94 (MQ=60)

                                                                                         ACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC         <  SRR4302541.1667306/94‑1 (MQ=60)

                                                                                         ACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC         <  SRR4302541.8515303/94‑1 (MQ=60)

                                                                                         ACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC         <  SRR4302541.7058829/94‑1 (MQ=60)

                                                                                         ACTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC         <  SRR4302541.3507878/94‑1 (MQ=60)

                                                                                          cctcaagG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTC         <  SRR4302541.7962617/87‑1 (MQ=60)

                                                                                          CTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        <  SRR4302541.1028460/94‑1 (MQ=60)

                                                                                          CTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        <  SRR4302541.8750450/94‑1 (MQ=60)

                                                                                          CTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        >  SRR4302541.1707631/1‑94 (MQ=60)

                                                                                          CTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        >  SRR4302541.7314453/1‑94 (MQ=60)

                                                                                          CTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        <  SRR4302541.6660819/94‑1 (MQ=60)

                                                                                          CTC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCG        <  SRR4302541.3865157/94‑1 (MQ=60)

                                                                                           TC‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGG       >  SRR4302541.719986/1‑94 (MQ=60)

                                                                                            C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGC      >  SRR4302541.7482303/1‑94 (MQ=60)

                                                                                            C‑AAGG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGC      <  SRR4302541.5752181/94‑1 (MQ=60)

                                                                                              aagG‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCT     <  SRR4302541.6103282/91‑1 (MQ=60)

                                                                                              AG‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTC    >  SRR4302541.8220436/1‑94 (MQ=60)

                                                                                               G‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC   >  SRR4302541.818241/1‑94 (MQ=60)

                                                                                               G‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC   <  SRR4302541.6336267/94‑1 (MQ=60)

                                                                                               G‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC   >  SRR4302541.7713761/1‑94 (MQ=60)

                                                                                               G‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCC   >  SRR4302541.1304286/1‑94 (MQ=60)

                                                                                                 G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  <  SRR4302541.8435762/94‑1 (MQ=60)

                                                                                                 G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  <  SRR4302541.6252774/94‑1 (MQ=60)

                                                                                                 G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  <  SRR4302541.3311785/94‑1 (MQ=60)

GACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCG‑‑A‑‑CTCTCCCACAGGGAGAGGGAGAAAACACTC‑AA‑G‑CCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCA  >  NC_000913/3957033‑3957217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`