Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,311 A→G N382S (AAC→AGC)  nagE → PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,3110AG100.0% 715.2 / NA 203N382S (AAC→AGC) nagEPTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (118/85);  total (118/85)

Only 100 of 215 total aligned reads displayed.

TCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT  >  NC_000913/704221‑704400
                                                                                          |                                                                                         
tCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCa                                                                                         >  1:1315400/1‑93 (MQ=255)
tCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCa                                                                                         <  1:2020627/93‑1 (MQ=255)
  gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCagcagc                                                                                       <  1:5683740/93‑1 (MQ=255)
   tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAgcagca                                                                                      >  1:5838517/1‑93 (MQ=255)
     gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAac                                                                                    >  1:6188203/1‑93 (MQ=255)
     gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAac                                                                                    <  1:2997441/93‑1 (MQ=255)
      tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAaca                                                                                   >  2:2376129/1‑93 (MQ=255)
       gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAacac                                                                                  >  2:5461856/1‑93 (MQ=255)
        ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACt                                                                                 <  2:2119251/93‑1 (MQ=255)
          cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTga                                                                               <  1:3800194/93‑1 (MQ=255)
           aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaa                                                                              <  2:3041217/93‑1 (MQ=255)
           aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaa                                                                              >  2:1782403/1‑93 (MQ=255)
           aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaa                                                                              >  1:1602984/1‑93 (MQ=255)
           aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAACATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaa                                                                              >  1:2292700/1‑93 (MQ=255)
               cggTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGaagaag                                                                          <  1:1880990/92‑1 (MQ=255)
               cggTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGaagaag                                                                          <  1:3914587/92‑1 (MQ=255)
                ggTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAgg                                                                         <  2:3411863/93‑1 (MQ=255)
                 gTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGTAGATAAAGAAGCCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAAGt                                                                        >  2:2268599/1‑93 (MQ=255)
                 gTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGCCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAggc                                                                        >  1:4610397/1‑92 (MQ=255)
                  ttATCCGCATTTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTc                                                                       <  2:4446913/93‑1 (MQ=255)
                  ttATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTc                                                                       <  2:2471283/93‑1 (MQ=255)
                  ttATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTc                                                                       <  2:5161769/93‑1 (MQ=255)
                   tATCGGAATGTACATCATAAAATCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCt                                                                      <  1:3998297/93‑1 (MQ=255)
                    aTCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTg                                                                     >  1:6057641/1‑93 (MQ=255)
                     tCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGa                                                                    <  2:1979045/93‑1 (MQ=255)
                       cGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACt                                                                  >  2:1955283/1‑93 (MQ=255)
                       cGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACt                                                                  >  2:4886305/1‑93 (MQ=255)
                        gCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAGGAAGGTCTGACTc                                                                 >  1:2760370/1‑93 (MQ=255)
                        gCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTc                                                                 >  1:4346144/1‑93 (MQ=255)
                          aTGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCaa                                                               >  1:988876/1‑93 (MQ=255)
                           tGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAc                                                              >  2:5228351/1‑93 (MQ=255)
                            gTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACt                                                             <  1:2087492/93‑1 (MQ=255)
                             ttCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTg                                                            >  1:5930470/1‑93 (MQ=255)
                             ttCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTg                                                            >  2:4038219/1‑93 (MQ=255)
                               cAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGc                                                          >  2:1723922/1‑93 (MQ=255)
                                aaCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGca                                                         <  2:1848918/93‑1 (MQ=255)
                                aaCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGca                                                         >  2:2509549/1‑93 (MQ=255)
                                 aCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaa                                                        >  2:2789112/1‑93 (MQ=255)
                                   cTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaacc                                                      >  1:1959160/1‑93 (MQ=255)
                                     gAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaaccaa                                                    <  2:5395679/93‑1 (MQ=255)
                                     gAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaaccaa                                                    >  2:4994524/1‑93 (MQ=255)
                                       aaaCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAAGACTGAAGAAGGTCTGACTCCACTGGCAACCAACt                                                  >  1:654077/1‑93 (MQ=255)
                                         aCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACtat                                                <  1:1271059/93‑1 (MQ=255)
                                         aCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACtat                                                >  2:5267118/1‑93 (MQ=255)
                                          cGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACtata                                               <  1:2376129/93‑1 (MQ=255)
                                           gCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTatat                                              <  1:2111388/93‑1 (MQ=255)
                                            ccGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATAtt                                             >  2:5804328/1‑93 (MQ=255)
                                             cGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtg                                            >  1:2285825/1‑93 (MQ=255)
                                             cGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtg                                            >  2:810259/1‑93 (MQ=255)
                                              gggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgc                                           <  2:1923251/93‑1 (MQ=255)
                                               ggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgct                                          <  2:2964791/93‑1 (MQ=255)
                                               ggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgct                                          <  1:5739958/93‑1 (MQ=255)
                                                 tCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgctgc                                        <  2:6176245/93‑1 (MQ=255)
                                                  cGTGAAAATAAAGAAGAGGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCg                                       <  1:1398798/93‑1 (MQ=255)
                                                   gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      <  1:3195429/93‑1 (MQ=255)
                                                   gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      >  2:4421857/1‑93 (MQ=255)
                                                   gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      <  1:4990706/93‑1 (MQ=255)
                                                   gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      >  2:4556733/1‑93 (MQ=255)
                                                    tGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGt                                     >  1:4242755/1‑93 (MQ=255)
                                                    tGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGt                                     >  2:2733513/1‑93 (MQ=255)
                                                    tGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGt                                     <  1:4504702/93‑1 (MQ=255)
                                                    tGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGt                                     <  2:2856159/93‑1 (MQ=255)
                                                     gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    <  2:3218392/93‑1 (MQ=255)
                                                       aGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTgg                                  <  2:5901938/93‑1 (MQ=255)
                                                       aGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTgg                                  >  1:5076919/1‑93 (MQ=255)
                                                        gATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggg                                 >  2:1193359/1‑92 (MQ=255)
                                                          tAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgg                               <  2:6032009/93‑1 (MQ=255)
                                                           aaaGAAGACGAGATCGTTACTGAAGAATCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcggc                              <  2:3618831/93‑1 (MQ=255)
                                                           aaaGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcggc                              >  2:5098523/1‑93 (MQ=255)
                                                           aaaGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgga                              >  2:5757430/1‑92 (MQ=255)
                                                            aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             >  1:679561/1‑93 (MQ=255)
                                                            aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             >  1:4202832/1‑93 (MQ=255)
                                                             agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAc                            >  2:888973/1‑93 (MQ=255)
                                                             agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAc                            <  2:3518248/93‑1 (MQ=255)
                                                             agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAc                            >  1:970123/1‑93 (MQ=255)
                                                              gaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACt                           <  2:6072584/93‑1 (MQ=255)
                                                               aagaCGAGATCGTTACTGAAGAAGCCAGCATCAACACTGAAGAAGGTCGGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTg                          <  2:680193/93‑1 (MQ=255)
                                                                 gaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAc                        >  2:5551702/1‑93 (MQ=255)
                                                                 gaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAc                        >  1:4853782/1‑93 (MQ=255)
                                                                   cGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACaa                      >  1:220601/1‑93 (MQ=255)
                                                                     agaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAAcc                    >  1:356656/1‑93 (MQ=255)
                                                                      gaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   >  1:3606041/1‑93 (MQ=255)
                                                                      gaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   >  1:6099207/1‑93 (MQ=255)
                                                                       aTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTg                  >  2:4800645/1‑93 (MQ=255)
                                                                         cGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGaa                <  2:2113444/93‑1 (MQ=255)
                                                                             aCTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCg            >  2:3380931/1‑93 (MQ=255)
                                                                               gtaagaagCCAGCAGCAACACTGAAGAAGGGCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAt          <  2:5637694/91‑1 (MQ=255)
                                                                                gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         <  2:3897345/93‑1 (MQ=255)
                                                                                gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         >  2:3582732/1‑93 (MQ=255)
                                                                                gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         >  2:42659/1‑93 (MQ=255)
                                                                                 aagaagCCAGCAGCAACACTGGAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTTCGGTTGGCGGCGCTGACAATATGGAAGCGGTTg        >  1:5530023/1‑93 (MQ=255)
                                                                                  agaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGa       >  2:4579823/1‑93 (MQ=255)
                                                                                  agaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGa       >  1:2478515/1‑93 (MQ=255)
                                                                                   gaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAc      >  1:3433673/1‑93 (MQ=255)
                                                                                     agCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGc    >  2:1880123/1‑93 (MQ=255)
                                                                                     agCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGc    >  2:2665184/1‑93 (MQ=255)
                                                                                      gCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGcc   <  1:533230/93‑1 (MQ=255)
                                                                                      gCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGcc   >  2:420923/1‑93 (MQ=255)
                                                                                       ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  <  1:5093533/93‑1 (MQ=255)
                                                                                       ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  <  1:4132437/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT  >  NC_000913/704221‑704400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTAT  >  NC_000913/704219‑704404
                                                                                            |                                                                                             
CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGC                                                                                              >  SRR4302534.928207/1‑94 (MQ=60)
CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGC                                                                                              >  SRR4302534.2555921/1‑94 (MQ=60)
CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGC                                                                                              >  SRR4302534.3953919/1‑94 (MQ=60)
CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGC                                                                                              <  SRR4302534.5397464/94‑1 (MQ=60)
CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGC                                                                                              >  SRR4302534.1921668/1‑94 (MQ=60)
 TTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCA                                                                                             >  SRR4302534.1350078/1‑94 (MQ=60)
  TCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAG                                                                                            <  SRR4302534.2073497/94‑1 (MQ=60)
  TCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAG                                                                                            >  SRR4302534.6186596/1‑94 (MQ=60)
    GTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCA                                                                                          >  SRR4302534.5997018/1‑94 (MQ=60)
    GTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCA                                                                                          <  SRR4302534.5837660/94‑1 (MQ=60)
     TGGTGTTCAGTTCGGTTATCCGCATGTTCACCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAA                                                                                         <  SRR4302534.3711519/94‑1 (MQ=60)
      GGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAAC                                                                                        >  SRR4302534.6356618/1‑94 (MQ=60)
       GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACA                                                                                       <  SRR4302534.1933821/94‑1 (MQ=60)
       GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACA                                                                                       <  SRR4302534.3075824/94‑1 (MQ=60)
        TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACAC                                                                                      >  SRR4302534.2021457/1‑94 (MQ=60)
         GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACT                                                                                     <  SRR4302534.6229985/94‑1 (MQ=60)
         GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCCTTACTGAAGAAGCCAGCAGCAACACT                                                                                     >  SRR4302534.5524046/1‑94 (MQ=60)
           TCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGA                                                                                   >  SRR4302534.6295796/1‑94 (MQ=60)
            CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAA                                                                                  >  SRR4302534.1644865/1‑94 (MQ=60)
            CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAA                                                                                  <  SRR4302534.3902232/94‑1 (MQ=60)
            CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAACATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAA                                                                                  >  SRR4302534.2353549/1‑94 (MQ=60)
                 CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGG                                                                             <  SRR4302534.5958146/94‑1 (MQ=60)
                 CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGG                                                                             <  SRR4302534.1929987/94‑1 (MQ=60)
                 CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGG                                                                             <  SRR4302534.4019541/94‑1 (MQ=60)
                  GGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGCCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGC                                                                            >  SRR4302534.4735534/1‑94 (MQ=60)
                  GGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGT                                                                            <  SRR4302534.1332741/94‑1 (MQ=60)
                    TTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCT                                                                          <  SRR4302534.6249/94‑1 (MQ=60)
                     tatcggaatgtacatcataaaatCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTG                                                                         <  SRR4302534.4105327/71‑1 (MQ=60)
                     TATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTG                                                                         >  SRR4302534.6222364/1‑94 (MQ=60)
                     TATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTG                                                                         <  SRR4302534.4695598/94‑1 (MQ=60)
                     TATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTG                                                                         <  SRR4302534.5829801/94‑1 (MQ=60)
                      ATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGA                                                                        <  SRR4302534.1902892/94‑1 (MQ=60)
                        CCGCATGTTGAACCTGAAAAGGCCGGGTAGTGAAGATAAAGAAGACAAGACCGGTACTTAAGAAGGCAGCAGCAACACTGAAGAAGGTCTGACT                                                                      >  SRR4302534.3661805/1‑94 (MQ=53)
                         CGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGTCAGCAGCAACACTGAAGAAGGTCTGACTC                                                                     >  SRR4302534.3004217/1‑94 (MQ=60)
                         CGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAGGAAGGTCTGACTC                                                                     >  SRR4302534.2833081/1‑94 (MQ=60)
                         CGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTC                                                                     >  SRR4302534.4463508/1‑94 (MQ=60)
                           CATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAA                                                                   >  SRR4302534.1014172/1‑94 (MQ=60)
                            ATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAC                                                                  <  SRR4302534.1002250/94‑1 (MQ=60)
                             TGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACT                                                                 <  SRR4302534.5612219/94‑1 (MQ=60)
                             TGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACT                                                                 <  SRR4302534.2151663/94‑1 (MQ=60)
                              GTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTG                                                                <  SRR4302534.2142204/94‑1 (MQ=60)
                              GTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTG                                                                >  SRR4302534.6091517/1‑94 (MQ=60)
                                TCACCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGC                                                              <  SRR4302534.1415155/94‑1 (MQ=60)
                                 CAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCA                                                             <  SRR4302534.5221671/94‑1 (MQ=60)
                                 CAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGACAGAAGCAACACTGAAGAAGGTCTGACTCAACTGGCA                                                             >  SRR4302534.2434290/1‑94 (MQ=60)
                                   ACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAAC                                                           >  SRR4302534.2728372/1‑94 (MQ=60)
                                    CCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACC                                                          >  SRR4302534.1490850/1‑94 (MQ=60)
                                    CCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACC                                                          >  SRR4302534.2010433/1‑94 (MQ=60)
                                    CCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACC                                                          >  SRR4302534.3512175/1‑94 (MQ=60)
                                       GAAAACGCCGGGTCGAGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGGCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAAC                                                       >  SRR4302534.1668719/1‑94 (MQ=60)
                                        AAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAAGACTGAAGAAGGTCTGACTCCACTGGCAACCAACT                                                      >  SRR4302534.670004/1‑94 (MQ=60)
                                         AAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTA                                                     >  SRR4302534.4267469/1‑94 (MQ=60)
                                           ACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATA                                                   <  SRR4302534.1304530/94‑1 (MQ=60)
                                            CGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATAT                                                  <  SRR4302534.2439229/94‑1 (MQ=60)
                                             GCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATT                                                 <  SRR4302534.2166908/94‑1 (MQ=60)
                                              CCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTG                                                >  SRR4302534.2346485/1‑94 (MQ=60)
                                                GGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCT                                              <  SRR4302534.2293358/94‑1 (MQ=60)
                                                GGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCT                                              <  SRR4302534.6054000/94‑1 (MQ=60)
                                                GGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCT                                              >  SRR4302534.2496010/1‑94 (MQ=60)
                                                GGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCT                                              >  SRR4302534.2170447/1‑94 (MQ=60)
                                                 GGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTG                                             <  SRR4302534.5895531/94‑1 (MQ=60)
                                                    CGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGG                                          <  SRR4302534.4744445/94‑1 (MQ=60)
                                                    CGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGG                                          >  SRR4302534.5127305/1‑94 (MQ=60)
                                                    CGTGAAAATAAAGAAGAGGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGG                                          <  SRR4302534.1435599/94‑1 (MQ=60)
                                                     GTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGT                                         >  SRR4302534.4357168/1‑94 (MQ=60)
                                                     GTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGT                                         <  SRR4302534.3280139/94‑1 (MQ=60)
                                                     GTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGT                                         >  SRR4302534.5093330/1‑94 (MQ=60)
                                                     GTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGT                                         <  SRR4302534.5125727/94‑1 (MQ=60)
                                                      TGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTT                                        <  SRR4302534.4626637/94‑1 (MQ=60)
                                                        AAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGG                                      >  SRR4302534.5213977/1‑94 (MQ=60)
                                                         AGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGC                                     >  SRR4302534.3664034/1‑94 (MQ=60)
                                                         AGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGC                                     >  SRR4302534.4149532/1‑94 (MQ=60)
                                                            aaaatAAGACGAGATCGTCACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGC                                  <  SRR4302534.459544/89‑1 (MQ=60)
                                                            TAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGC                                  >  SRR4302534.5864052/1‑94 (MQ=60)
                                                             AAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCA                                 >  SRR4302534.696158/1‑94 (MQ=60)
                                                             AAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCA                                 >  SRR4302534.4316301/1‑94 (MQ=60)
                                                              AAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAC                                >  SRR4302534.994841/1‑94 (MQ=60)
                                                              AAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAC                                >  SRR4302534.2352860/1‑94 (MQ=60)
                                                               AGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACT                               >  SRR4302534.2775888/1‑94 (MQ=60)
                                                                  AGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAC                            >  SRR4302534.6378687/1‑94 (MQ=60)
                                                                  AGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAC                            >  SRR4302534.4009497/1‑94 (MQ=60)
                                                                  AGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAC                            >  SRR4302534.4985469/1‑94 (MQ=60)
                                                                    ACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAA                          >  SRR4302534.226385/1‑94 (MQ=60)
                                                                     CGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAAC                         >  SRR4302534.682796/1‑94 (MQ=60)
                                                                      GAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACC                        <  SRR4302534.4327927/94‑1 (MQ=60)
                                                                      GAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACC                        >  SRR4302534.365781/1‑94 (MQ=60)
                                                                      GAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACC                        >  SRR4302534.3721098/1‑94 (MQ=60)
                                                                       AGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCT                       >  SRR4302534.3703010/1‑94 (MQ=60)
                                                                       AGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCT                       >  SRR4302534.6265058/1‑94 (MQ=60)
                                                                        GATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTG                      >  SRR4302534.784631/1‑94 (MQ=60)
                                                                             TTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGC                 >  SRR4302534.3600542/1‑94 (MQ=60)
                                                                                CTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAT              >  SRR4302534.4394158/1‑94 (MQ=60)
                                                                                  GAAGAAGCCAGCAGCAACACTGGAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTTCGGTTGGCGGCGCTGACAAtatggaagcggttg            >  SRR4302534.5679283/1‑80 (MQ=60)
                                                                                   AAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGA           >  SRR4302534.2544360/1‑94 (MQ=60)
                                                                                    AGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAC          >  SRR4302534.2088913/1‑94 (MQ=60)
                                                                                    AGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAC          >  SRR4302534.3525929/1‑94 (MQ=60)
                                                                                     GAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACG         >  SRR4302534.211092/1‑94 (MQ=60)
                                                                                     AAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACG         <  SRR4302534.4498936/94‑1 (MQ=60)
                                                                                      AAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGC        >  SRR4302534.3192409/1‑94 (MQ=60)
                                                                                       AGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCC       <  SRR4302534.5304648/94‑1 (MQ=60)
                                                                                        GCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT      <  SRR4302534.546470/94‑1 (MQ=60)
                                                                                        GCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT      <  SRR4302534.4090009/94‑1 (MQ=60)
                                                                                         CCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTG     <  SRR4302534.4243866/94‑1 (MQ=60)
                                                                                         CCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTG     >  SRR4302534.5711782/1‑94 (MQ=60)
                                                                                         CCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTG     >  SRR4302534.439179/1‑94 (MQ=60)
                                                                                         CCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTG     <  SRR4302534.5231023/94‑1 (MQ=60)
                                                                                          CAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGT    >  SRR4302534.1785447/1‑94 (MQ=60)
                                                                                          CAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGT    >  SRR4302534.1803552/1‑94 (MQ=60)
                                                                                           AGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTA   >  SRR4302534.4136912/1‑94 (MQ=60)
                                                                                            GCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTAT  >  SRR4302534.3110016/1‑94 (MQ=60)
                                                                                            |                                                                                             
CTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTAT  >  NC_000913/704219‑704404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑