Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,311 A→G N382S (AAC→AGC)  nagE → PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,3110AG100.0% 792.2 / NA 229N382S (AAC→AGC) nagEPTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (141/88);  total (141/88)

Only 100 of 245 total aligned reads displayed.

GTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT  >  NC_000913/704223‑704400
                                                                                        |                                                                                         
gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCcagcaga                                                                                       >  1:5480442/1‑92 (MQ=255)
gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCagcagc                                                                                       >  1:1107658/1‑93 (MQ=255)
gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCagcagc                                                                                       >  2:6820298/1‑93 (MQ=255)
 tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAgcagca                                                                                      <  1:3344263/93‑1 (MQ=255)
 tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAgcagca                                                                                      >  1:1027235/1‑93 (MQ=255)
 cggtgTTAAGTTCGGTTATCCGCATGTTCACCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAgcagca                                                                                      <  2:7257535/92‑1 (MQ=255)
    tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAaca                                                                                   <  1:80443/93‑1 (MQ=255)
     gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAacac                                                                                  <  2:1789511/93‑1 (MQ=255)
      ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGGGATCGTTACTGAAGAAGCCAGCAGCAACACt                                                                                 >  1:5936019/1‑93 (MQ=255)
      ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACt                                                                                 >  1:5863705/1‑93 (MQ=255)
      ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACt                                                                                 >  1:4963836/1‑93 (MQ=255)
           ttcggTTATCCGCATGCTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaaga                                                                            >  1:4577007/4‑93 (MQ=255)
               gTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAggc                                                                        >  2:369464/1‑92 (MQ=255)
               gTTATCCGCATGTTCAACCTGAAAACGACGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGACGGt                                                                        >  2:8713649/1‑93 (MQ=255)
                 tATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTcg                                                                      >  1:7210549/1‑92 (MQ=255)
                  aTCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTg                                                                     >  2:5087057/1‑93 (MQ=255)
                   tCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGa                                                                    >  2:3321538/1‑93 (MQ=255)
                    ccGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGAc                                                                   <  1:4475938/93‑1 (MQ=255)
                      gCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTc                                                                 >  2:2859492/1‑93 (MQ=255)
                       cATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGCAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCa                                                                >  1:5720657/1‑93 (MQ=255)
                       cATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCa                                                                >  1:6039059/1‑93 (MQ=255)
                         tGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAc                                                              >  2:1407924/1‑93 (MQ=255)
                         tGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAc                                                              <  2:3646687/93‑1 (MQ=255)
                         tGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAc                                                              >  2:4111373/1‑93 (MQ=255)
                            tCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTgg                                                           >  1:655044/1‑93 (MQ=255)
                              aaCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGca                                                         >  2:133923/1‑93 (MQ=255)
                               aCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTgcgag                                                        >  1:4132169/1‑89 (MQ=255)
                               aCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaa                                                        >  2:1573366/1‑93 (MQ=255)
                                 cTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaacc                                                      <  1:5844779/93‑1 (MQ=255)
                                 cTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaacc                                                      >  2:8086191/1‑93 (MQ=255)
                                  tGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGTAGGTCTGACTCAACTGGcaacca                                                     >  2:4754343/1‑93 (MQ=255)
                                   gAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaaccaa                                                    >  2:3220348/1‑93 (MQ=255)
                                   gAAAACGCCGGGCCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaatcaa                                                    >  2:4056319/1‑93 (MQ=255)
                                     aaaCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACt                                                  >  1:4206036/1‑93 (MQ=255)
                                     aaaCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACt                                                  <  2:7102229/93‑1 (MQ=255)
                                      aaCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACta                                                 <  2:795536/93‑1 (MQ=255)
                                          ccGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATAtt                                             >  2:8599524/1‑93 (MQ=255)
                                          ccGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATAtt                                             >  2:7386034/1‑93 (MQ=255)
                                           cGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtg                                            >  2:6480488/1‑93 (MQ=255)
                                            gggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgc                                           >  2:6966238/1‑93 (MQ=255)
                                            gggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgc                                           <  2:4821380/93‑1 (MQ=255)
                                            gggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgc                                           >  2:8751793/1‑93 (MQ=255)
                                             ggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgct                                          <  1:4893590/93‑1 (MQ=255)
                                                cGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCg                                       >  2:6667009/1‑93 (MQ=255)
                                                 gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      >  2:7694494/1‑93 (MQ=255)
                                                 gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      <  1:3109030/93‑1 (MQ=255)
                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    >  2:5257716/1‑93 (MQ=255)
                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    <  1:5274823/93‑1 (MQ=255)
                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    <  1:2459031/93‑1 (MQ=255)
                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    >  1:6807123/1‑93 (MQ=255)
                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    >  1:5235128/1‑93 (MQ=255)
                                                    aaGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTg                                   <  2:7466425/93‑1 (MQ=255)
                                                    aaGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTg                                   <  1:3447785/93‑1 (MQ=255)
                                                    aaGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTg                                   <  1:7748048/93‑1 (MQ=255)
                                                      gATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggg                                 >  1:3203638/1‑92 (MQ=255)
                                                      gATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggc                                 >  2:6222639/1‑93 (MQ=255)
                                                      gATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggc                                 <  2:1206722/93‑1 (MQ=255)
                                                        tAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgg                               >  1:6122191/1‑93 (MQ=255)
                                                        tAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgg                               >  1:2356890/1‑93 (MQ=255)
                                                        tAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgg                               <  1:6908695/93‑1 (MQ=255)
                                                         aaaGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcggc                              <  1:3891692/93‑1 (MQ=255)
                                                         aaaGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcggc                              <  2:8360129/93‑1 (MQ=255)
                                                          aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             >  2:1614907/1‑93 (MQ=255)
                                                          aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             <  2:1434122/93‑1 (MQ=255)
                                                          aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             <  1:2925843/93‑1 (MQ=255)
                                                          aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             <  2:7228977/93‑1 (MQ=255)
                                                           agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCCACCAACTATATTGCTGCGGTTGGCGGCAc                            <  2:5367241/93‑1 (MQ=255)
                                                           agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAc                            <  2:1497327/93‑1 (MQ=255)
                                                            gaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACt                           >  2:19828/1‑93 (MQ=255)
                                                            gaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACt                           <  2:1765342/93‑1 (MQ=255)
                                                              agaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGa                         <  1:1849040/93‑1 (MQ=255)
                                                               gaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAc                        >  1:7070068/1‑93 (MQ=255)
                                                                   agaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAAcc                    >  2:2292102/1‑93 (MQ=255)
                                                                    gaTCTTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   <  2:4399886/93‑1 (MQ=255)
                                                                    gaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   <  2:824528/93‑1 (MQ=255)
                                                                    gaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   >  1:5577500/1‑93 (MQ=255)
                                                                     aTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTg                  >  1:716663/1‑93 (MQ=255)
                                                                     aTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTg                  >  1:8620245/1‑93 (MQ=255)
                                                                         ttACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTTTATTGCTGCGGTTGGCGGCACTGACAACCTGAAAg              >  1:6464278/1‑93 (MQ=255)
                                                                           aCTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGca            >  2:6011383/1‑92 (MQ=255)
                                                                           aCTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCg            >  1:4024479/1‑93 (MQ=255)
                                                                             tGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAt          >  1:3060898/1‑93 (MQ=255)
                                                                             tGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAt          <  1:8305416/93‑1 (MQ=255)
                                                                              gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         <  2:1262267/93‑1 (MQ=255)
                                                                              gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         >  2:2368467/1‑93 (MQ=255)
                                                                              gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         >  2:5680801/1‑93 (MQ=255)
                                                                               aagaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTg        >  1:6789181/1‑93 (MQ=255)
                                                                               aagaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTg        >  1:6262659/1‑93 (MQ=255)
                                                                               aagaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTg        >  2:2556760/1‑93 (MQ=255)
                                                                                agaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGa       <  2:8520304/93‑1 (MQ=255)
                                                                                agaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGa       >  2:1705525/1‑93 (MQ=255)
                                                                                 gaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAc      >  1:7321808/1‑93 (MQ=255)
                                                                                 gaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAc      <  2:2746060/93‑1 (MQ=255)
                                                                                 gaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAc      >  1:471860/1‑93 (MQ=255)
                                                                                  aagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACg     >  1:2101577/1‑93 (MQ=255)
                                                                                  aagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACg     >  1:135650/1‑93 (MQ=255)
                                                                                    gCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGcc   >  1:2015823/1‑93 (MQ=255)
                                                                                     ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  >  2:6171302/1‑93 (MQ=255)
                                                                                     ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  >  2:100510/1‑93 (MQ=255)
                                                                                     ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  >  2:5518176/1‑93 (MQ=255)
                                                                                        |                                                                                         
GTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT  >  NC_000913/704223‑704400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTA  >  NC_000913/704218‑704403
                                                                                             |                                                                                            
ACTTCGTGGTGTTCAGTTCGGTTATCCGCATCTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAG                                                                                              <  SRR4302541.8203225/94‑1 (MQ=60)
 CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGC                                                                                             >  SRR4302541.4859260/1‑94 (MQ=60)
 CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGC                                                                                             >  SRR4302541.5263324/1‑94 (MQ=60)
    CGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGC                                                                                          >  SRR4302541.3835470/1‑94 (MQ=60)
    CGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGC                                                                                          >  SRR4302541.1118052/1‑94 (MQ=60)
    CGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGC                                                                                          >  SRR4302541.524765/1‑94 (MQ=60)
     GTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCA                                                                                         >  SRR4302541.1036724/1‑94 (MQ=60)
     GTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCA                                                                                         <  SRR4302541.6255898/94‑1 (MQ=60)
      TGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAA                                                                                        <  SRR4302541.3377701/94‑1 (MQ=60)
       GGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAAC                                                                                       >  SRR4302541.3285293/1‑94 (MQ=60)
       GGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAAC                                                                                       <  SRR4302541.5911569/94‑1 (MQ=60)
       GGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAAC                                                                                       <  SRR4302541.1657997/94‑1 (MQ=60)
       GGGGTTGAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAAC                                                                                       <  SRR4302541.5911570/94‑1 (MQ=60)
         TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACAC                                                                                     <  SRR4302541.81134/94‑1 (MQ=60)
          tgtcagttgtGTTATCCGCATCTCCAACCTGAAAACGCCTGGTCGTGAAGATAAGGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACT                                                                                    <  SRR4302541.30838/84‑1 (MQ=60)
          GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGGGATCGTTACTGAAGAAGCCAGCAGCAACACT                                                                                    >  SRR4302541.5996494/1‑94 (MQ=60)
          GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACT                                                                                    <  SRR4302541.6172835/94‑1 (MQ=60)
          GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACT                                                                                    >  SRR4302541.5923354/1‑94 (MQ=60)
          GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACT                                                                                    >  SRR4302541.5014660/1‑94 (MQ=60)
             CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAA                                                                                 >  SRR4302541.6979978/1‑94 (MQ=60)
               GTTCGGTTATCCGCATGCTCAGCCTGAAAACGCCGGGTCGTGAAGATACAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAACA                                                                               >  SRR4302541.4623380/1‑94 (MQ=60)
               GTTCGGTTATCCGCATGCTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGA                                                                               >  SRR4302541.4623381/1‑94 (MQ=60)
                 TCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAG                                                                             >  SRR4302541.8725897/1‑94 (MQ=60)
                  CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGG                                                                            >  SRR4302541.7252552/1‑94 (MQ=60)
                   GGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGT                                                                           >  SRR4302541.6628122/1‑94 (MQ=60)
                    GTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTC                                                                          <  SRR4302541.4989324/94‑1 (MQ=60)
                    GTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTC                                                                          >  SRR4302541.4165720/1‑94 (MQ=60)
                    GTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTC                                                                          >  SRR4302541.4165719/1‑94 (MQ=60)
                     TTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCT                                                                         <  SRR4302541.2473004/94‑1 (MQ=60)
                     TTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCG                                                                         >  SRR4302541.7287467/1‑94 (MQ=60)
                      TATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTG                                                                        <  SRR4302541.4368757/94‑1 (MQ=60)
                       ATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGA                                                                       >  SRR4302541.4463811/1‑94 (MQ=60)
                       ATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGA                                                                       >  SRR4302541.1056127/1‑94 (MQ=60)
                         CCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACT                                                                     <  SRR4302541.270996/94‑1 (MQ=60)
                         CCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACT                                                                     <  SRR4302541.4521042/94‑1 (MQ=60)
                           GCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGCAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCA                                                                   >  SRR4302541.5778971/1‑94 (MQ=60)
                           GCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCA                                                                   >  SRR4302541.6100874/1‑94 (MQ=60)
                           GCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCA                                                                   >  SRR4302541.4187927/1‑94 (MQ=60)
                            CATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAC                                                                  >  SRR4302541.2455101/1‑94 (MQ=60)
                            CATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAA                                                                  >  SRR4302541.8412932/1‑94 (MQ=60)
                            CATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTAAAGAAGGTCTGACTCAA                                                                  >  SRR4302541.6507542/1‑94 (MQ=60)
                             ATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAC                                                                 >  SRR4302541.8906407/1‑94 (MQ=60)
                             ATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAC                                                                 >  SRR4302541.6475264/1‑94 (MQ=60)
                              TGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACT                                                                <  SRR4302541.6171091/94‑1 (MQ=60)
                               GTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTG                                                               >  SRR4302541.3330093/1‑94 (MQ=60)
                                TTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGG                                                              >  SRR4302541.661225/1‑94 (MQ=60)
                                TTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGA                                                              >  SRR4302541.2068196/1‑94 (MQ=60)
                                   AACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGcgag                                                           >  SRR4302541.4172987/1‑90 (MQ=60)
                                      CTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCA                                                        <  SRR4302541.5904211/94‑1 (MQ=60)
                                      CTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCA                                                        <  SRR4302541.5904210/94‑1 (MQ=60)
                                      CTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCA                                                        >  SRR4302541.223902/1‑94 (MQ=60)
                                         AAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACT                                                     >  SRR4302541.4247854/1‑94 (MQ=60)
                                         AAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACT                                                     >  SRR4302541.5499470/1‑94 (MQ=60)
                                          AAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTA                                                    <  SRR4302541.1922987/94‑1 (MQ=60)
                                          AAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTA                                                    >  SRR4302541.8249692/1‑94 (MQ=60)
                                          AAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTA                                                    <  SRR4302541.5504880/94‑1 (MQ=60)
                                            ACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATA                                                  >  SRR4302541.3011912/1‑94 (MQ=60)
                                             CGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATAT                                                 <  SRR4302541.7304347/94‑1 (MQ=60)
                                                CGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGC                                              >  SRR4302541.5923623/1‑94 (MQ=60)
                                                CGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGC                                              >  SRR4302541.4521394/1‑94 (MQ=60)
                                                 GGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCT                                             <  SRR4302541.7817961/94‑1 (MQ=60)
                                                  GGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTG                                            <  SRR4302541.4943521/94‑1 (MQ=60)
                                                   GTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGC                                           <  SRR4302541.7083995/94‑1 (MQ=60)
                                                     CGTGAAGATAAAGAAGACTAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGG                                         >  SRR4302541.5771430/1‑94 (MQ=60)
                                                     CGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGG                                         >  SRR4302541.118056/1‑94 (MQ=60)
                                                      GTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGT                                        <  SRR4302541.3140503/94‑1 (MQ=60)
                                                       TGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTT                                       >  SRR4302541.5289066/1‑94 (MQ=60)
                                                       TGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTT                                       >  SRR4302541.6879925/1‑94 (MQ=60)
                                                       TGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTT                                       >  SRR4302541.6879926/1‑94 (MQ=60)
                                                        GAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTG                                      <  SRR4302541.5329152/94‑1 (MQ=60)
                                                        GAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTG                                      <  SRR4302541.4690866/94‑1 (MQ=60)
                                                        GAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTG                                      <  SRR4302541.2483550/94‑1 (MQ=60)
                                                         AAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGG                                     <  SRR4302541.7830488/94‑1 (MQ=60)
                                                         AAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGG                                     <  SRR4302541.3482236/94‑1 (MQ=60)
                                                         AAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGG                                     >  SRR4302541.337956/1‑94 (MQ=60)
                                                          AGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGG                                    >  SRR4302541.3236000/1‑94 (MQ=60)
                                                          AGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGC                                    <  SRR4302541.3115193/94‑1 (MQ=60)
                                                            ATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGG                                  >  SRR4302541.6184721/1‑94 (MQ=60)
                                                            ATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGG                                  >  SRR4302541.2379845/1‑94 (MQ=60)
                                                             TAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGC                                 <  SRR4302541.6982423/94‑1 (MQ=60)
                                                              AAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCA                                <  SRR4302541.3930695/94‑1 (MQ=60)
                                                               AAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAC                               <  SRR4302541.2955262/94‑1 (MQ=60)
                                                               AAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAC                               <  SRR4302541.5066878/94‑1 (MQ=60)
                                                               AAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAC                               >  SRR4302541.5575890/1‑94 (MQ=60)
                                                                AGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACT                              <  SRR4302541.7975963/94‑1 (MQ=60)
                                                                AGAAGACGAGATCGTGACGGAAGAAGCCAGCAGCAACACTGAAGACGGTCGGCCTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACT                              <  SRR4302541.7975965/94‑1 (MQ=60)
                                                                 GAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTG                             >  SRR4302541.4690310/1‑94 (MQ=60)
                                                                   AGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGca                           >  SRR4302541.7145254/1‑92 (MQ=60)
                                                                   AGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAC                           <  SRR4302541.1866921/94‑1 (MQ=60)
                                                                   AGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAC                           >  SRR4302541.7145253/1‑94 (MQ=60)
                                                                    GACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACA                          <  SRR4302541.5314781/94‑1 (MQ=60)
                                                                     ACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAA                         >  SRR4302541.691489/1‑94 (MQ=60)
                                                                       GAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACC                       >  SRR4302541.7829340/1‑94 (MQ=60)
                                                                        AGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCT                      >  SRR4302541.5634594/1‑94 (MQ=60)
                                                                         GATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTG                     >  SRR4302541.4041390/1‑94 (MQ=60)
                                                                         GATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTG                     >  SRR4302541.723426/1‑94 (MQ=60)
                                                                         GATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTG                     >  SRR4302541.8712661/1‑94 (MQ=60)
                                                                           TCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAA                   <  SRR4302541.7230242/94‑1 (MQ=60)
                                                                             GTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTTTATTGCTGCGGTTGGCGGCACTGACAACCTGAAAG                 >  SRR4302541.6530153/1‑94 (MQ=60)
                                                                             GTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAG                 <  SRR4302541.3789988/94‑1 (MQ=60)
                                                                              TTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGC                <  SRR4302541.5745171/94‑1 (MQ=60)
                                                                               TACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCG               >  SRR4302541.4064244/1‑94 (MQ=60)
                                                                                 CTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAT             >  SRR4302541.3091910/1‑94 (MQ=60)
                                                                                 CTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAT             >  SRR4302541.6548598/1‑94 (MQ=60)
                                                                                  TGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATT            <  SRR4302541.8395714/94‑1 (MQ=60)
                                                                                  TGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATT            <  SRR4302541.8381452/94‑1 (MQ=60)
                                                                                  TGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATT            <  SRR4302541.1720988/94‑1 (MQ=60)
                                                                                   GAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTG           >  SRR4302541.6861778/1‑94 (MQ=60)
                                                                                   GAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTG           >  SRR4302541.6326614/1‑94 (MQ=60)
                                                                                    AAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGA          >  SRR4302541.3182292/1‑94 (MQ=60)
                                                                                     AGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGCAAGCGATTGAC         >  SRR4302541.6018873/1‑94 (MQ=60)
                                                                                     AGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAC         >  SRR4302541.7399989/1‑94 (MQ=60)
                                                                                     AGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAC         >  SRR4302541.4928674/1‑94 (MQ=60)
                                                                                     AGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAC         >  SRR4302541.476343/1‑94 (MQ=60)
                                                                                      GAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACG        >  SRR4302541.2121949/1‑94 (MQ=60)
                                                                                      GAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACG        >  SRR4302541.136705/1‑94 (MQ=60)
                                                                                       AAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGC       >  SRR4302541.2036942/1‑94 (MQ=60)
                                                                                        AGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCC      >  SRR4302541.2035336/1‑94 (MQ=60)
                                                                                        AGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCC      <  SRR4302541.6693474/94‑1 (MQ=60)
                                                                                           CAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGT   >  SRR4302541.4739603/1‑94 (MQ=60)
                                                                                            AGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTA  >  SRR4302541.4721738/1‑94 (MQ=60)
                                                                                            AGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTA  >  SRR4302541.249079/1‑94 (MQ=60)
                                                                                            AGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTA  >  SRR4302541.7381616/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCTGTA  >  NC_000913/704218‑704403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑