Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	729,698	A→G	H298R (CAC→CGC)	rhsC →	RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	729,698	0	A	G	90.4%	155.6 / 11.7	73	H298R (CAC→CGC)	rhsC	RhsC
Reads supporting (aligned to +/- strand): ref base A (6/1); new base G (40/26); total (46/27)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACC  >  NC_000913/729667‑729787

ggCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑ccc                              >  2:769956/1‑93 (MQ=14)

ggCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑ccc                              <  2:3870388/93‑1 (MQ=14)

ggCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑ccc                              >  2:1838634/1‑93 (MQ=14)

ggCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑ccc                              >  1:5340499/1‑93 (MQ=14)

 gCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑cccc                             >  2:5189826/1‑93 (MQ=17)

 gCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑cccc                             >  2:4256475/1‑93 (MQ=17)

 gCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑cccc                             >  2:1552476/1‑93 (MQ=17)

 gCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑cccc                             >  2:535133/1‑93 (MQ=32)

  cATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGAGG‑GCCcg                            >  1:3546213/1‑93 (MQ=255)

   aTCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCcgc                           >  2:1045460/1‑93 (MQ=18)

   aTCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCcgc                           <  2:169047/93‑1 (MQ=18)

   aTCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCcgc                           <  1:6093501/93‑1 (MQ=18)

    tCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCcgcg                          >  2:5826458/1‑93 (MQ=18)

    tCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCcgcg                          >  1:3061001/1‑93 (MQ=18)

    tCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCcgcg                          >  2:1975887/1‑93 (MQ=18)

    tCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCcgcg                          >  1:1916296/1‑93 (MQ=18)

    tCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCcgcg                          >  1:4600432/1‑93 (MQ=33)

      cGTCTGTCTGCCGTGTGGCTGCCGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCgcggc                        <  2:3313707/93‑1 (MQ=18)

      cGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCgcggc                        >  2:2726025/1‑93 (MQ=18)

       gtctgtctGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGGCG‑CCCCgcggcg                       >  2:5701262/1‑93 (MQ=255)

       gtctgtctGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGGCG‑CCCCgcggca                       >  2:1798169/1‑92 (MQ=255)

       gtctgtctGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCgcggcg                       >  1:1348442/1‑93 (MQ=33)

       gtctgtctGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCgcggcg                       >  1:3149377/1‑93 (MQ=33)

       gtctgtctGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGAAG‑GCCCgcggcg                       >  1:2349130/1‑93 (MQ=16)

        tctgtctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGa                      <  2:3135409/93‑1 (MQ=21)

         ctgtctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGaa                     <  1:4267020/93‑1 (MQ=21)

          tgtctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAAc                    <  1:3916737/93‑1 (MQ=18)

          tgtctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGGGCGCTATGGCTGGGCG‑CCCCcccgcggaa                    >  1:1383298/1‑90 (MQ=11)

           gtctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACt                   <  1:5364074/93‑1 (MQ=21)

           gtctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACt                   >  1:3206867/1‑93 (MQ=18)

            tctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTg                  >  1:1065850/1‑93 (MQ=18)

             ctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGGCG‑CCCCCCGGCGAACttg                 >  1:249884/1‑91 (MQ=11)

             ctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTgg                 >  2:6103009/1‑93 (MQ=18)

             ctgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTgg                 >  1:5059267/1‑93 (MQ=18)

              tgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTggc                <  1:1538657/93‑1 (MQ=21)

              tgCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTggc                <  1:2372438/93‑1 (MQ=21)

              tgCCGTGTGGCTGACGCACGACCCGGAATACCCGGCGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑GCCCCCCGCGAACGggc                >  1:3251028/1‑93 (MQ=11)

                ccGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTggcgg              <  1:2984458/93‑1 (MQ=21)

                 cGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGGCG‑CCCCCCGGCGAACcggcggg             >  1:1472344/1‑92 (MQ=11)

                 cGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACT‑CCCGGGGGCGAACTggcggg             >  2:5323369/1‑92 (MQ=11)

                 cGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTggcggc             <  2:1290368/93‑1 (MQ=25)

                  gtgtGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑GCCCGCCGCGGACCTgcggcg            >  2:3567353/1‑93 (MQ=11)

                  gtgtGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGgcggcg            >  1:899545/1‑93 (MQ=25)

                   tgtgGCTGCCGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGcggcgg           <  1:2986515/93‑1 (MQ=255)

                   tgtgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGcggcgg           >  2:4101648/1‑93 (MQ=255)

                   tgtgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGcggcgg           <  1:5828216/93‑1 (MQ=255)

                   tgtgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGcggcgg           <  1:5846031/93‑1 (MQ=255)

                    gtgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGcggcggg          >  1:2678695/1‑92 (MQ=255)

                    gtgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGgt          <  2:575230/93‑1 (MQ=255)

                    gtgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGgt          <  2:6177442/93‑1 (MQ=255)

                     tgGCTGACGCGCGACCCGGAATCCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGgtg         <  1:3368112/93‑1 (MQ=255)

                     tgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGgtg         <  2:4570919/93‑1 (MQ=37)

                     tgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGgtg         <  2:2743402/93‑1 (MQ=255)

                     tgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGgtg         <  2:339531/93‑1 (MQ=37)

                     tgGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCCCCGCGAACTGGcggcgggg         >  1:3660907/1‑91 (MQ=11)

                      ggCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGcggcggggg        >  2:1451817/1‑90 (MQ=255)

                      ggCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGgtgt        <  2:3483621/93‑1 (MQ=255)

                      ggCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGgtgt        >  1:6173668/1‑93 (MQ=255)

                      ggCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCCCGGCCAACCGGCGGCGgcgt        >  2:2818190/1‑93 (MQ=11)

                       gCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTa       <  1:78154/93‑1 (MQ=37)

                        cTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTAt      <  1:5740258/93‑1 (MQ=255)

                        cTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTAt      <  2:2383867/93‑1 (MQ=255)

                        cTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTAt      <  1:5425153/93‑1 (MQ=255)

                         tGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGGGCTATGGCTGGGCG‑CCCCGCGGCGCACTGGCGGCGGGGTATg     >  2:6153286/1‑93 (MQ=11)

                         tGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATg     <  2:5897440/93‑1 (MQ=37)

                         tGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATg     >  1:2154606/1‑93 (MQ=255)

                         tGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATg     >  2:6153287/1‑93 (MQ=255)

                          gACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGTTG‑CCCCGCGGCGAACGGGCGGGGGGGTagtg    >  2:491598/1‑90 (MQ=11)

                          gACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGa    >  2:5886707/1‑93 (MQ=255)

                          gACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGa    >  2:4060360/1‑93 (MQ=255)

                          gACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGa    >  1:1883798/1‑93 (MQ=255)

                          gACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGa    >  2:2130427/1‑93 (MQ=37)

                          gACGCGCGACCCGAAATACCCGGAGAATTTACCTGCCGCGCCGCTCGAGCGCTATGGCTGGACGACCCCCCGGGGAA‑TGGCGGCGGGGTATGa    >  1:300911/1‑93 (MQ=11)

                           aCGCGCGACCCGGAATACCCGGAGAATTTGCCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGAc   <  2:1845094/93‑1 (MQ=255)

                           aCGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGAc   <  1:4898286/93‑1 (MQ=255)

                            cgcgcgACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGAcc  <  1:4442691/93‑1 (MQ=255)

                            cGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGAcc  <  2:2457496/93‑1 (MQ=38)

GGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACC  >  NC_000913/729667‑729787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACCGCA  >  NC_000913/729605‑729790

GGACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCG                                                                                               >  SRR4302534.6162872/1‑94 (MQ=0)

 GACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCAC                                                                                              >  SRR4302534.3145743/1‑94 (MQ=0)

  ACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACAGCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACG                                                                                             >  SRR4302534.752747/1‑94 (MQ=0)

  ACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACG                                                                                             <  SRR4302534.5049820/94‑1 (MQ=0)

  ACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACG                                                                                             <  SRR4302534.5189511/94‑1 (MQ=0)

  ACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACG                                                                                             >  SRR4302534.3667048/1‑94 (MQ=0)

    GGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGAC                                                                                           >  SRR4302534.3688462/1‑94 (MQ=0)

    GGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGAC                                                                                           >  SRR4302534.5520504/1‑94 (MQ=0)

    GGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGAC                                                                                           >  SRR4302534.5849875/1‑94 (MQ=0)

      AACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCC                                                                                         <  SRR4302534.6599901/94‑1 (MQ=0)

       ACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCG                                                                                        >  SRR4302534.513339/1‑94 (MQ=0)

       ACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCG                                                                                        >  SRR4302534.4440085/1‑94 (MQ=0)

        CCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGG                                                                                       <  SRR4302534.1213986/94‑1 (MQ=0)

        CCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGG                                                                                       <  SRR4302534.4534541/94‑1 (MQ=0)

        CCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGG                                                                                       >  SRR4302534.2738105/1‑94 (MQ=0)

        CCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGG                                                                                       <  SRR4302534.352050/94‑1 (MQ=0)

         CGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGA                                                                                      >  SRR4302534.3900462/1‑94 (MQ=0)

         CGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGA                                                                                      >  SRR4302534.4855613/1‑94 (MQ=0)

          GTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAA                                                                                     >  SRR4302534.987278/1‑94 (MQ=0)

           TCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAAT                                                                                    <  SRR4302534.3990104/94‑1 (MQ=0)

            CCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATA                                                                                   <  SRR4302534.1894864/94‑1 (MQ=0)

               CTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCC                                                                                >  SRR4302534.6229199/1‑94 (MQ=0)

               CTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCC                                                                                >  SRR4302534.5447340/1‑94 (MQ=0)

               CTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCC                                                                                >  SRR4302534.6321431/1‑94 (MQ=0)

               CTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGAACCGGAATACCC                                                                                >  SRR4302534.5160401/1‑94 (MQ=0)

                TTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCG                                                                               <  SRR4302534.5994115/94‑1 (MQ=0)

                TTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCG                                                                               >  SRR4302534.1819104/1‑94 (MQ=0)

                 TTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGG                                                                              <  SRR4302534.5163712/94‑1 (MQ=0)

                   TCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAG                                                                            >  SRR4302534.256293/1‑94 (MQ=0)

                    CCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGA                                                                           >  SRR4302534.806997/1‑94 (MQ=0)

                    CCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGA                                                                           >  SRR4302534.2192556/1‑94 (MQ=0)

                    CCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCCTGTGGCTGACGCGCGACCCGGAATACCCGGAGA                                                                           >  SRR4302534.1109245/1‑94 (MQ=0)

                     CTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAA                                                                          >  SRR4302534.4214523/1‑94 (MQ=0)

                       GATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATT                                                                        >  SRR4302534.851643/1‑94 (MQ=0)

                       GATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATT                                                                        >  SRR4302534.3315627/1‑94 (MQ=0)

                        ATACCCTGCCGGGTTACGCCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTT                                                                       <  SRR4302534.2205521/94‑1 (MQ=0)

                         TACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCCGAGAATTTA                                                                      >  SRR4302534.2039945/1‑94 (MQ=0)

                          ACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTAC                                                                     <  SRR4302534.6520025/94‑1 (MQ=0)

                          ACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTAC                                                                     >  SRR4302534.2417798/1‑94 (MQ=0)

                          ACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTAC                                                                     >  SRR4302534.2541791/1‑94 (MQ=0)

                          ACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTAC                                                                     >  SRR4302534.1950533/1‑94 (MQ=0)

                           CCCTGCCGTGTTCCACCGAATATGGCCGGGACAACGGCTTCCGTCTGTCTGCCGTGTGGCAGACGCACGACCCGGAATACCCGGAGAATTTACC                                                                    <  SRR4302534.407546/94‑1 (MQ=0)

                             CTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTG                                                                  <  SRR4302534.5709897/94‑1 (MQ=0)

                             CTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTG                                                                  >  SRR4302534.2838012/1‑94 (MQ=0)

                             CTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTAACTG                                                                  >  SRR4302534.2356378/1‑94 (MQ=0)

                              TGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGC                                                                 >  SRR4302534.3799551/1‑94 (MQ=0)

                               GCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCC                                                                <  SRR4302534.4359400/94‑1 (MQ=0)

                                CCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCG                                                               >  SRR4302534.2349515/1‑94 (MQ=0)

                                CCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCG                                                               >  SRR4302534.2978257/1‑94 (MQ=0)

                                CCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATCTACCTGCCG                                                               >  SRR4302534.5173819/1‑94 (MQ=0)

                                 CGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGC                                                              >  SRR4302534.763108/1‑94 (MQ=0)

                                 CGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGC                                                              >  SRR4302534.1917388/1‑94 (MQ=0)

                                 CGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTAACTGCCGC                                                              >  SRR4302534.341373/1‑94 (MQ=0)

                                  GGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCG                                                             <  SRR4302534.4022247/94‑1 (MQ=0)

                                   GGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGC                                                            >  SRR4302534.4030812/1‑94 (MQ=0)

                                   GGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGC                                                            <  SRR4302534.5979223/94‑1 (MQ=0)

                                    GTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCC                                                           >  SRR4302534.1539812/1‑94 (MQ=0)

                                     TTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCG                                                          <  SRR4302534.3143799/94‑1 (MQ=0)

                                     TTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCG                                                          <  SRR4302534.2367822/94‑1 (MQ=0)

                                     TTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCG                                                          >  SRR4302534.5494250/1‑94 (MQ=0)

                                     TTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCG                                                          <  SRR4302534.4112582/94‑1 (MQ=0)

                                      TACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGC                                                         >  SRR4302534.3595097/1‑94 (MQ=0)

                                      TACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGC                                                         <  SRR4302534.1043471/94‑1 (MQ=0)

                                       ACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCT                                                        >  SRR4302534.3453326/1‑94 (MQ=0)

                                        CGCCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATCCCCGGAGAATTTACCTGCCGCGCCGCTG                                                       <  SRR4302534.5654229/94‑1 (MQ=0)

                                          CCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGG                                                     >  SRR4302534.4682049/1‑94 (MQ=0)

                                           CGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTG                                                    >  SRR4302534.1615691/1‑94 (MQ=0)

                                           CGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTG                                                    <  SRR4302534.6339840/94‑1 (MQ=0)

                                            GAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGC                                                   <  SRR4302534.276144/94‑1 (MQ=0)

                                            GAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGC                                                   <  SRR4302534.2178276/94‑1 (MQ=0)

                                             AATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCG                                                  <  SRR4302534.1063456/94‑1 (MQ=0)

                                              ATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGC                                                 >  SRR4302534.3220537/1‑94 (MQ=0)

                                              ATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGC                                                 >  SRR4302534.6063104/1‑94 (MQ=0)

                                               TATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCT                                                >  SRR4302534.3583200/1‑94 (MQ=0)

                                                 TGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATCCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTAT                                              <  SRR4302534.5420972/94‑1 (MQ=0)

                                                  GGCCGGGACACCGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATG                                             <  SRR4302534.141342/94‑1 (MQ=0)

                                                  GGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATG                                             >  SRR4302534.2163009/1‑94 (MQ=0)

                                                  GGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATG                                             >  SRR4302534.3031245/1‑94 (MQ=0)

                                                  GGCCGGAACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATG                                             <  SRR4302534.3919578/94‑1 (MQ=0)

                                                   GCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGG                                            <  SRR4302534.5928824/94‑1 (MQ=0)

                                                   GCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGG                                            <  SRR4302534.3865311/94‑1 (MQ=0)

                                                   GCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGG                                            >  SRR4302534.4651931/1‑94 (MQ=0)

                                                    CCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGC                                           <  SRR4302534.3347100/94‑1 (MQ=0)

                                                     CGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCT                                          >  SRR4302534.734399/1‑94 (MQ=0)

                                                      GGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTG                                         >  SRR4302534.1207465/1‑94 (MQ=0)

                                                       GGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGG                                        >  SRR4302534.2468371/1‑94 (MQ=0)

                                                       GGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGG                                        >  SRR4302534.3903920/1‑94 (MQ=0)

                                                        GACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGA                                       >  SRR4302534.6581050/1‑94 (MQ=0)

                                                        GACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGGGCGCTATGGCTGGG                                       >  SRR4302534.1829012/1‑94 (MQ=0)

                                                         ACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCccgggcgccctgggtcggg                                      >  SRR4302534.47939/1‑75 (MQ=0)

                                                         ACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGAC                                      <  SRR4302534.6064883/94‑1 (MQ=0)

                                                          CAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG                                     >  SRR4302534.2257448/1‑94 (MQ=0)

                                                          CAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG                                     <  SRR4302534.3324091/94‑1 (MQ=0)

                                                          CAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG                                     >  SRR4302534.1143796/1‑94 (MQ=0)

                                                           AACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGGGCGCTATGGCTGGACG‑C                                   >  SRR4302534.3103450/1‑94 (MQ=0)

                                                             CGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCC                                 >  SRR4302534.5484481/1‑94 (MQ=0)

                                                             CGGCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCC                                 <  SRR4302534.6037659/94‑1 (MQ=0)

                                                              GGCATCCGTATGTCTGCCGTGTGGCTGACGCGCGACCCGGAATAACCGGAGAATTTACCTGCCGCGCCGCTGTTGCGCAATGGCTGGAGG‑CCCC                                >  SRR4302534.902532/1‑94 (MQ=0)

                                                               GCATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGAGG‑GCCCG                               >  SRR4302534.3641590/1‑94 (MQ=0)

                                                                 ATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCG                             >  SRR4302534.3141327/1‑94 (MQ=8)

                                                                 ATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCG                             <  SRR4302534.6259200/94‑1 (MQ=8)

                                                                 ATCCGTCTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCG                             >  SRR4302534.1966339/1‑94 (MQ=8)

                                                                 ATCCGTCTGTCTGCCGTGTGGCTGACGCATGACCCCGAATACCCGGAGCATTTACATGCCGCGCCGCTGGTGCGCTATTGCTGGAgtcgccgcg                              >  SRR4302534.1549961/1‑85 (MQ=0)

                                                                 ATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCG                             >  SRR4302534.4725294/1‑94 (MQ=8)

                                                                    CGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCG                          >  SRR4302534.1383981/1‑94 (MQ=8)

                                                                    CGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCG                          >  SRR4302534.3232238/1‑94 (MQ=8)

                                                                    CGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGAAG‑GCCCGCGGCG                          >  SRR4302534.2411472/1‑94 (MQ=0)

                                                                       CTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAAC                       <  SRR4302534.4382299/94‑1 (MQ=8)

                                                                       CTGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGGGCGCTATGGCTGGGCG‑CCCCcccgcggaa                       >  SRR4302534.1419716/1‑85 (MQ=0)

                                                                        TGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACT                      <  SRR4302534.4021733/94‑1 (MQ=8)

                                                                        TGTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACT                      >  SRR4302534.3291913/1‑94 (MQ=8)

                                                                         GTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTG                     >  SRR4302534.1093480/1‑94 (MQ=8)

                                                                         GTCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTG                     <  SRR4302534.5508639/94‑1 (MQ=8)

                                                                          TCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGGCG‑CCCCCCGGCGAACTTG                    >  SRR4302534.256402/1‑94 (MQ=6)

                                                                          TCTGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGG                    >  SRR4302534.5195908/1‑94 (MQ=8)

                                                                            TGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCG                  <  SRR4302534.1578999/94‑1 (MQ=8)

                                                                            TGCCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCG                  <  SRR4302534.2435437/94‑1 (MQ=8)

                                                                              CCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGGCG‑CCCCCCGGCGAACCGGCGGG                >  SRR4302534.1511075/1‑94 (MQ=6)

                                                                              CCGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGC                <  SRR4302534.3062524/94‑1 (MQ=10)

                                                                               CGTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCG               >  SRR4302534.922344/1‑94 (MQ=13)

                                                                                GTGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCgtgtggggccttggagggagggcccccggcagagcagggggg               >  SRR4302534.5613821/1‑52 (MQ=0)

                                                                                 TGTGGCTGCCGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGT             <  SRR4302534.3064622/94‑1 (MQ=22)

                                                                                 TGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGT             <  SRR4302534.6004749/94‑1 (MQ=23)

                                                                                 TGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGT             <  SRR4302534.5986406/94‑1 (MQ=23)

                                                                                 TGTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGG             >  SRR4302534.2749508/1‑94 (MQ=20)

                                                                                  GTGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCCCCGCGAACTGGCGGCGGGG            >  SRR4302534.3759298/1‑94 (MQ=18)

                                                                                   TGGCTGACGCGCGACCCGGAATCCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGT           <  SRR4302534.3458348/94‑1 (MQ=24)

                                                                                   TGGCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGT           >  SRR4302534.6341629/1‑94 (MQ=25)

                                                                                     GCTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTAT         <  SRR4302534.80267/94‑1 (MQ=25)

                                                                                      CTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATG        >  SRR4302534.2211483/1‑94 (MQ=25)

                                                                                      CTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATG        <  SRR4302534.5571154/94‑1 (MQ=25)

                                                                                      CTGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATG        <  SRR4302534.5895842/94‑1 (MQ=25)

                                                                                       TGACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGA       >  SRR4302534.1932886/1‑94 (MQ=25)

                                                                                       TGACGCGCGACCCGAAATACCCGGAGAATTTACCTGCCGCGCCGCTCGAGCGCTATGGCTGGACGACCCCCCGGGGAA‑TGGCGGCGGGGTATGA       >  SRR4302534.308680/1‑94 (MQ=0)

                                                                                         ACGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACC     <  SRR4302534.5031085/94‑1 (MQ=25)

                                                                                          CGCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACCG    <  SRR4302534.4562706/94‑1 (MQ=25)

                                                                                           GCGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACCGC   <  SRR4302534.1736937/94‑1 (MQ=28)

                                                                                            CGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACCGCA  <  SRR4302534.4920058/94‑1 (MQ=31)

                                                                                            CGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACCGCA  <  SRR4302534.518090/94‑1 (MQ=31)

                                                                                            CGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGAAG‑CCCCGCGGggaaaaatcgggggtgctttacgccc  >  SRR4302534.4690873/1‑68 (MQ=9)

                                                                                            CGCGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGGGCGCTATGGCTGGGCG‑CCCCccgggggacctggggggggggatgaacgca  >  SRR4302534.4496722/1‑64 (MQ=0)

GGACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACG‑CCCCGCGGCGAACTGGCGGCGGTGTATGACCGCA  >  NC_000913/729605‑729790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`