Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,180	A→G	D120G (GAT→GGT)	ycfJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,180	0	A	G	99.5%	740.4 / ‑4.8	213	D120G (GAT→GGT)	ycfJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (96/116); total (96/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 217 total aligned reads displayed.

TGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGA  >  NC_000913/1167091‑1167270

tggtCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTAc                                                                                         <  2:1543495/93‑1 (MQ=255)

  gtCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACtt                                                                                       >  2:3722239/1‑93 (MQ=255)

   tCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTa                                                                                      <  1:911348/93‑1 (MQ=255)

    cgcgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTac                                                                                     <  2:5996906/93‑1 (MQ=255)

     gcgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTaca                                                                                    <  2:5265915/93‑1 (MQ=255)

     gcgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTaca                                                                                    >  2:2233533/1‑93 (MQ=255)

      cgGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTacac                                                                                   <  2:1357311/93‑1 (MQ=255)

       ggTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTGTCTCCAGGAAAGCGGTACTTACACg                                                                                  >  2:3767539/1‑93 (MQ=255)

         tAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGAc                                                                                >  1:4608671/1‑93 (MQ=255)

           aaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTa                                                                              >  2:1438779/1‑93 (MQ=255)

           aaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTa                                                                              <  2:4484774/93‑1 (MQ=255)

           aaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTa                                                                              <  2:4460085/93‑1 (MQ=255)

           aaGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTa                                                                              >  2:382790/1‑93 (MQ=255)

            aGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTAc                                                                             >  1:3468300/1‑93 (MQ=255)

            aGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTAc                                                                             >  1:384731/1‑93 (MQ=255)

             gATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACg                                                                            <  2:2030830/93‑1 (MQ=255)

                gTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCaa                                                                         <  2:4849116/93‑1 (MQ=255)

                 tCGCCACTTGTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAAc                                                                        <  2:5047592/93‑1 (MQ=255)

                 tCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAAc                                                                        <  2:3525449/93‑1 (MQ=255)

                   gCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTAAGCAACAg                                                                      >  2:2602354/1‑93 (MQ=255)

                   gCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGATAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAg                                                                      <  2:3029096/93‑1 (MQ=255)

                     cACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCg                                                                    >  1:1787874/1‑93 (MQ=255)

                      aCTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGt                                                                   <  2:3035154/93‑1 (MQ=255)

                      aCTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGt                                                                   <  1:2013148/93‑1 (MQ=255)

                      aCTGTGGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGt                                                                   <  2:5548401/93‑1 (MQ=255)

                       cTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCTAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGtt                                                                  <  1:125020/93‑1 (MQ=255)

                       cTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGtt                                                                  >  2:4697255/1‑93 (MQ=255)

                       cTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGtt                                                                  <  2:449953/93‑1 (MQ=255)

                       cTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGtt                                                                  <  2:4060321/93‑1 (MQ=255)

                         gttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGt                                                                <  1:272114/93‑1 (MQ=255)

                          ttgtGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTa                                                               <  1:1626040/93‑1 (MQ=255)

                             tgGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTaaaa                                                            <  1:85443/93‑1 (MQ=255)

                              gggggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAAc                                                           <  2:2159995/93‑1 (MQ=255)

                               ggggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACg                                                          <  2:5448130/93‑1 (MQ=255)

                               ggggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACg                                                          >  2:115088/1‑93 (MQ=255)

                                gggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACgg                                                         <  2:460686/93‑1 (MQ=255)

                                 ggCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGgt                                                        <  2:5855721/93‑1 (MQ=255)

                                  gcgcTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGgtg                                                       <  2:3266728/93‑1 (MQ=255)

                                  gcgcTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGgtg                                                       >  2:1760037/1‑93 (MQ=255)

                                  gcgcTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGgtg                                                       <  2:145229/93‑1 (MQ=255)

                                      tGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCGAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATg                                                   <  1:5788872/93‑1 (MQ=255)

                                       gggTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGa                                                  >  1:1374778/1‑93 (MQ=255)

                                       gggTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGa                                                  >  1:3610472/1‑93 (MQ=255)

                                        ggtggtGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGAc                                                 <  1:2123708/93‑1 (MQ=255)

                                          tggtggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACaa                                               >  2:6283187/1‑93 (MQ=255)

                                          tggtggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACaa                                               <  2:2375517/93‑1 (MQ=255)

                                            gtggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGt                                             <  2:3341573/93‑1 (MQ=255)

                                              ggATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCa                                           <  2:1955427/93‑1 (MQ=255)

                                                 tatGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaa                                        <  1:4734610/93‑1 (MQ=255)

                                                 tatGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaa                                        <  2:3471393/93‑1 (MQ=255)

                                                   tGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaaaa                                      >  2:4807500/1‑93 (MQ=255)

                                                    gCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaaaaa                                     >  1:330789/1‑93 (MQ=255)

                                                     ccGGTAACGAGATCCAGGGCTATCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGaaaaaa                                    <  1:5518827/93‑1 (MQ=255)

                                                      cGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAAt                                   <  2:4118983/93‑1 (MQ=255)

                                                       ggTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATg                                  <  2:2988869/93‑1 (MQ=255)

                                                       cgTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATg                                  <  2:5301125/92‑1 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 <  2:3351794/93‑1 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 <  1:2244972/93‑1 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 <  1:664882/93‑1 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 >  2:5827847/1‑93 (MQ=255)

                                                        gTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGc                                 <  2:1595012/93‑1 (MQ=255)

                                                         tAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGCGTATGACAAGTCAGAAAAAAAGCt                                >  1:5462756/1‑93 (MQ=255)

                                                          aaCCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTc                               >  2:1213210/1‑93 (MQ=255)

                                                          aaCCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTc                               >  2:3388205/1‑93 (MQ=255)

                                                           aCCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCg                              <  2:676121/93‑1 (MQ=255)

                                                            ccAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCgg                             >  2:5658981/1‑93 (MQ=255)

                                                            ccAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCgg                             >  1:263330/1‑93 (MQ=255)

                                                             cAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGt                            >  1:4402379/1‑93 (MQ=255)

                                                             cAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGt                            <  2:607784/93‑1 (MQ=255)

                                                               gATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTa                          >  2:3650182/1‑93 (MQ=255)

                                                                aTCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTat                         <  2:2403309/93‑1 (MQ=255)

                                                                 tCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatg                        <  2:342335/93‑1 (MQ=255)

                                                                  ccAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatga                       >  1:2553729/1‑93 (MQ=255)

                                                                   cAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatgat                      <  2:1299829/93‑1 (MQ=255)

                                                                    aGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatgatg                     >  1:5037746/1‑93 (MQ=255)

                                                                    aGGGCGCTATCCAGGAAAGCGGTACTTGCGCGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTatgatg                     <  1:1936562/93‑1 (MQ=255)

                                                                     gggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAATTCAGAAAAAATGCTCGGTTATGAtgt                    >  1:5202812/1‑93 (MQ=255)

                                                                     gggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAtgt                    <  2:5410938/93‑1 (MQ=255)

                                                                     gggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAtgt                    <  2:226177/93‑1 (MQ=255)

                                                                      ggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAtgtg                   >  1:5423058/1‑93 (MQ=255)

                                                                      ggCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAtgtg                   >  1:1575480/1‑93 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                <  2:5904389/93‑1 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                >  2:75896/1‑93 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                >  1:204218/1‑93 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                <  1:294223/93‑1 (MQ=255)

                                                                         tctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAcc                <  1:3409176/93‑1 (MQ=255)

                                                                          ctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCt               >  1:1921838/1‑93 (MQ=255)

                                                                          ctctcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCt               >  1:5235776/1‑93 (MQ=255)

                                                                             tcCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCtata            <  2:1712952/93‑1 (MQ=255)

                                                                               cAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg          <  2:5783953/93‑1 (MQ=255)

                                                                               cAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg          <  1:169656/93‑1 (MQ=255)

                                                                               cAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg          >  1:4344340/1‑93 (MQ=255)

                                                                               cAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAg          >  1:5063305/1‑93 (MQ=255)

                                                                                  gAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGAtt       <  1:3185150/93‑1 (MQ=255)

                                                                                   aaaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTg      <  1:3357499/93‑1 (MQ=255)

                                                                                   aaaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTg      >  1:5339187/1‑93 (MQ=255)

                                                                                   aaaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTg      <  1:1436838/93‑1 (MQ=255)

                                                                                    aaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTgg     >  2:2809841/1‑93 (MQ=255)

                                                                                    aaGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTgg     >  1:6309977/1‑93 (MQ=255)

                                                                                      gCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCg   <  1:5932034/93‑1 (MQ=255)

TGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGA  >  NC_000913/1167091‑1167270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATC  >  NC_000913/1167087‑1167272

GTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGG                                                                                              <  SRR4302534.5819239/94‑1 (MQ=60)

 TGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGT                                                                                             >  SRR4302534.3147871/1‑94 (MQ=60)

  GGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTA                                                                                            <  SRR4302534.4398692/94‑1 (MQ=60)

       TCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTAC                                                                                       <  SRR4302534.934524/94‑1 (MQ=60)

       TCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTAC                                                                                       >  SRR4302534.3274990/1‑94 (MQ=60)

          CGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACG                                                                                    >  SRR4302534.5332549/1‑94 (MQ=60)

          CGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACG                                                                                    >  SRR4302534.1910243/1‑94 (MQ=60)

           GGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGA                                                                                   >  SRR4302534.3416668/1‑94 (MQ=60)

            GTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGAC                                                                                  >  SRR4302534.4733766/1‑94 (MQ=60)

              AAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTA                                                                                >  SRR4302534.2208320/1‑94 (MQ=60)

              AAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTA                                                                                <  SRR4302534.4159706/94‑1 (MQ=60)

               AAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTAC                                                                               >  SRR4302534.3561518/1‑94 (MQ=60)

               AAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTAC                                                                               >  SRR4302534.394573/1‑94 (MQ=60)

                  ATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCA                                                                            >  SRR4302534.2007315/1‑94 (MQ=60)

                    GTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAAC                                                                          <  SRR4302534.5638674/94‑1 (MQ=60)

                      CGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAG                                                                        <  SRR4302534.779345/94‑1 (MQ=60)

                      CGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAG                                                                        >  SRR4302534.5347616/1‑94 (MQ=60)

                       GCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGC                                                                       <  SRR4302534.1590529/94‑1 (MQ=60)

                        CCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCG                                                                      >  SRR4302534.1834297/1‑94 (MQ=60)

                        CCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACGACGCAACAGCG                                                                      <  SRR4302534.3366223/94‑1 (MQ=60)

                         CACTGTTGTGGTGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGCGCTCTCTCCAGGAAAGCGGTCCTTACCCGACTTCGCATCAGCGT                                                                     <  SRR4302534.905558/94‑1 (MQ=60)

                         CACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGT                                                                     >  SRR4302534.3917356/1‑94 (MQ=60)

                          ACTGTTGTGGTGGTGCTGGGTGGTGGATATGCCGGTAACCAGATCAAGGGCTCTCTCCAGGAAAGCGGTACTTACCAGACTACGCAACAGCGTT                                                                    <  SRR4302534.4931813/94‑1 (MQ=60)

                          ACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTT                                                                    <  SRR4302534.2065820/94‑1 (MQ=60)

                          ACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTT                                                                    >  SRR4302534.1641501/1‑94 (MQ=60)

                           CTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCTAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTG                                                                   <  SRR4302534.128323/94‑1 (MQ=60)

                           CTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTG                                                                   >  SRR4302534.50891/1‑94 (MQ=60)

                           CTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTG                                                                   >  SRR4302534.3525413/1‑94 (MQ=60)

                           CTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTG                                                                   <  SRR4302534.2284321/94‑1 (MQ=60)

                             GTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTA                                                                 >  SRR4302534.2334631/1‑94 (MQ=60)

                             GTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTA                                                                 <  SRR4302534.279162/94‑1 (MQ=60)

                              TTGTGGGGGCGCTGGGTGGTGGATATGCGGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAA                                                                <  SRR4302534.5896274/94‑1 (MQ=60)

                              TTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAA                                                                <  SRR4302534.1668466/94‑1 (MQ=60)

                               TGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAA                                                               >  SRR4302534.3486989/1‑94 (MQ=60)

                                 TGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAAC                                                             <  SRR4302534.87724/94‑1 (MQ=60)

                                   GGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGG                                                           <  SRR4302534.5604041/94‑1 (MQ=60)

                                   GGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGG                                                           >  SRR4302534.1792444/1‑94 (MQ=60)

                                    GGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGT                                                          <  SRR4302534.5179417/94‑1 (MQ=60)

                                       CGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTA                                                       >  SRR4302534.131194/1‑94 (MQ=60)

                                       CGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTA                                                       <  SRR4302534.5426779/94‑1 (MQ=60)

                                          TGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCGAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGA                                                    <  SRR4302534.5945913/94‑1 (MQ=60)

                                          TGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGA                                                    >  SRR4302534.1411001/1‑94 (MQ=60)

                                          TGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGA                                                    >  SRR4302534.3707562/1‑94 (MQ=60)

                                            GGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACA                                                  <  SRR4302534.2179626/94‑1 (MQ=60)

                                             GTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAA                                                 <  SRR4302534.1975991/94‑1 (MQ=60)

                                              TGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAG                                                <  SRR4302534.2838466/94‑1 (MQ=60)

                                                  GGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAG                                            <  SRR4302534.5905788/94‑1 (MQ=60)

                                                     TATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAA                                         <  SRR4302534.4863164/94‑1 (MQ=60)

                                                      ATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAA                                        >  SRR4302534.3694139/1‑94 (MQ=60)

                                                       TGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAA                                       >  SRR4302534.339295/1‑94 (MQ=60)

                                                        GCCGATAACCAGATCCAGGGCTCTCTCCAGAAGAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCGGAAAAAA                                      <  SRR4302534.1646342/94‑1 (MQ=60)

                                                         CCGGTAACGAGATCCAGGGCTATCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAAT                                     <  SRR4302534.5667739/94‑1 (MQ=60)

                                                            GTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCT                                  <  SRR4302534.2304474/94‑1 (MQ=60)

                                                            GTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCT                                  <  SRR4302534.681068/94‑1 (MQ=60)

                                                            GTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGCGTATGACAAGTCAGAAAAAAAGCT                                  >  SRR4302534.5609994/1‑94 (MQ=60)

                                                              AACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCG                                <  SRR4302534.1421746/94‑1 (MQ=60)

                                                              AACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCG                                >  SRR4302534.2151344/1‑94 (MQ=60)

                                                               ACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGG                               >  SRR4302534.270160/1‑94 (MQ=60)

                                                               ACCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGG                               >  SRR4302534.481975/1‑94 (MQ=60)

                                                                CCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGT                              >  SRR4302534.4081104/1‑94 (MQ=60)

                                                                CCAGATCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGT                              >  SRR4302534.4521160/1‑94 (MQ=60)

                                                                     TCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCTGTTATGA                         <  SRR4302534.5507201/94‑1 (MQ=60)

                                                                     TCCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGA                         >  SRR4302534.2621426/1‑94 (MQ=60)

                                                                      CCAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGAT                        >  SRR4302534.2654541/1‑94 (MQ=60)

                                                                       CAGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATG                       >  SRR4302534.5173808/1‑94 (MQ=60)

                                                                        AGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAATTCAGAAAAAATGCTCGGTTATGATGT                      >  SRR4302534.5343169/1‑94 (MQ=60)

                                                                        AGGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGT                      >  SRR4302534.2084171/1‑94 (MQ=60)

                                                                        AGGGCGCTATCCAGGAAAGCGGTACTTGCGCGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGT                      <  SRR4302534.1987194/94‑1 (MQ=60)

                                                                         GGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTG                     >  SRR4302534.1616704/1‑94 (MQ=60)

                                                                         GGGCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTG                     >  SRR4302534.5568999/1‑94 (MQ=60)

                                                                           GCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAC                   >  SRR4302534.461225/1‑94 (MQ=60)

                                                                           GCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAC                   >  SRR4302534.5972303/1‑94 (MQ=60)

                                                                           GCTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGAC                   >  SRR4302534.5311570/1‑94 (MQ=60)

                                                                           GCNCTCTNNANGAAAGCGGTACTNNNNCNNCTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATNATGTGAC                   <  SRR4302534.2531366/94‑1 (MQ=60)

                                                                            CTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACC                  >  SRR4302534.209578/1‑94 (MQ=60)

                                                                            CTCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACC                  >  SRR4302534.30782/1‑94 (MQ=60)

                                                                             TCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCT                 >  SRR4302534.5377138/1‑94 (MQ=60)

                                                                             TCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCT                 >  SRR4302534.1972077/1‑94 (MQ=60)

                                                                             TCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCT                 <  SRR4302534.1725744/94‑1 (MQ=60)

                                                                             TCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCT                 <  SRR4302534.301833/94‑1 (MQ=60)

                                                                             TCTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCT                 <  SRR4302534.3500742/94‑1 (MQ=60)

                                                                              CTCTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGACGTGACCTA                <  SRR4302534.6530840/94‑1 (MQ=60)

                                                                                CTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATA              >  SRR4302534.4498054/1‑94 (MQ=60)

                                                                                CTCCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATA              >  SRR4302534.6154295/1‑94 (MQ=60)

                                                                                  CCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAG            >  SRR4302534.5200027/1‑94 (MQ=60)

                                                                                  CCAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAG            >  SRR4302534.4461681/1‑94 (MQ=60)

                                                                                   CAGGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGA           <  SRR4302534.174131/94‑1 (MQ=60)

                                                                                     GGAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATT         >  SRR4302534.1800141/1‑94 (MQ=60)

                                                                                      GAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTG        >  SRR4302534.5483139/1‑94 (MQ=60)

                                                                                      GAAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTG        <  SRR4302534.3269385/94‑1 (MQ=60)

                                                                                       AAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGG       >  SRR4302534.2729885/1‑94 (MQ=60)

                                                                                       AAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGG       <  SRR4302534.1474658/94‑1 (MQ=60)

                                                                                       AAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGG       <  SRR4302534.3447430/94‑1 (MQ=60)

                                                                                       AAAGCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGG       >  SRR4302534.6480569/1‑94 (MQ=60)

                                                                                          GCGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGA    <  SRR4302534.6093122/94‑1 (MQ=60)

                                                                                           CGGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGAT   <  SRR4302534.2527004/94‑1 (MQ=60)

                                                                                            GGTACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGAAC  >  SRR4302534.4045321/1‑94 (MQ=60)

GTGGTGGTCGCGGTAAAGATGTCGCCACTGTTGTGGGGGCGCTGGGTGGTGGATATGCCGGTAACCAGATCCAGGGCTCTCTCCAGGAAAGCGATACTTACACGACTACGCAACAGCGTTGTAAAACGGTGTATGACAAGTCAGAAAAAATGCTCGGTTATGATGTGACCTATAAGATTGGCGATC  >  NC_000913/1167087‑1167272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`