Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,175,607	A→G	S320G (AGC→GGC)	ycfU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,175,607	0	A	G	98.9%	892.6 / ‑0.8	268	S320G (AGC→GGC)	ycfU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/3); new base G (133/132); total (133/135)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 278 total aligned reads displayed.

GTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTC  >  NC_000913/1175517‑1175696

gTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGgc                                                                                         <  2:2976402/93‑1 (MQ=255)

gTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGgc                                                                                         >  1:3536941/1‑93 (MQ=255)

  aaTGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGgcgc                                                                                       <  1:1766474/93‑1 (MQ=255)

   agggAGTATCATGGCGAAGTATCGATCCTGCAAACGCAAGGCTTAACTCCGCGACATATCATGATGTTCTTTATGGTGCAAGGTTCCGGCGcc                                                                                      >  2:7800593/3‑93 (MQ=255)

   aTGGAGAAGCAGGGCGAAGTAGCGATCCTGCACACGCAAGGCTTAACTCCGCGACCAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGcc                                                                                      >  1:5226346/1‑93 (MQ=255)

   aTGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGcc                                                                                      <  2:2331232/93‑1 (MQ=255)

   aTGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGcc                                                                                      >  1:2725642/1‑93 (MQ=255)

    tGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACGCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCg                                                                                     >  2:5182320/1‑93 (MQ=255)

     ggAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCggg                                                                                    <  2:6862706/93‑1 (MQ=255)

        gaAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGAt                                                                                 >  1:1381819/1‑93 (MQ=255)

          aGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTa                                                                               >  1:5609285/1‑93 (MQ=255)

          aGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTa                                                                               >  1:8148722/1‑93 (MQ=255)

          aGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTa                                                                               >  1:3087072/1‑93 (MQ=255)

           gCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTAt                                                                              >  2:6619232/1‑93 (MQ=255)

           gCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTAt                                                                              <  1:7057541/93‑1 (MQ=255)

              gtgagAAGTAGCGATCCTTCAAACGCAAGGCTTAACTCCGCTACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCgg                                                                           <  2:6403012/89‑1 (MQ=255)

                 cGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGc                                                                        <  1:3335564/93‑1 (MQ=255)

                     gTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATc                                                                    <  2:4045331/93‑1 (MQ=255)

                     gTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATc                                                                    >  1:7314024/1‑93 (MQ=255)

                      tAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATcc                                                                   <  2:4762688/93‑1 (MQ=255)

                      tAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATcc                                                                   <  1:4305030/93‑1 (MQ=255)

                      tAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATcc                                                                   >  2:903876/1‑93 (MQ=255)

                       aGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCt                                                                  <  1:1722086/93‑1 (MQ=255)

                        gCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTc                                                                 <  1:1592911/93‑1 (MQ=255)

                          gATCCTGCAAACGCAAGGCTTACCTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCgg                                                               <  1:8753776/93‑1 (MQ=255)

                           aTCCTGCAAACGCAAGGCTTAACTGCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGa                                                              >  2:8450240/1‑93 (MQ=255)

                           aTCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGa                                                              >  1:2130840/1‑93 (MQ=255)

                             ccTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAgc                                                            >  2:1485627/1‑93 (MQ=255)

                             ccTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAgc                                                            <  2:105411/93‑1 (MQ=255)

                               tGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAgcgg                                                          >  2:2058617/1‑93 (MQ=255)

                               tGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAgcgg                                                          >  1:892250/1‑93 (MQ=255)

                                gCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAgcggc                                                         <  2:8765867/93‑1 (MQ=255)

                                  aaaCGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGgcgc                                                       >  2:3457087/1‑93 (MQ=255)

                                  aaaCGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGgcgc                                                       <  2:5901372/93‑1 (MQ=255)

                                   aaCGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCt                                                      <  1:6409706/93‑1 (MQ=255)

                                   aaCGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCt                                                      <  2:4872065/93‑1 (MQ=255)

                                    aCGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCtt                                                     >  1:1095781/1‑93 (MQ=255)

                                    aCGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCtt                                                     >  1:7342509/1‑93 (MQ=255)

                                    aCGCAAGGCTTAACTCCGCGACAAATCATGATGATCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCtt                                                     <  2:3747051/93‑1 (MQ=255)

                                       cAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGgc                                                  <  1:4505225/93‑1 (MQ=255)

                                         aGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGgcgc                                                >  2:7538979/1‑93 (MQ=255)

                                          ggCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGcc                                               >  1:6928190/1‑93 (MQ=255)

                                          ggCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGcc                                               <  2:1573829/93‑1 (MQ=255)

                                             ttAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTTGGAGCGGCGCTTGGCGCCCTg                                            >  2:8320410/1‑93 (MQ=255)

                                             ttAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTg                                            >  2:2411764/1‑93 (MQ=255)

                                             ttAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTg                                            >  1:1189314/1‑93 (MQ=255)

                                               aaCTCCGGTACAACTTATGATGGTCTTTAGGGTGCAAGGGGCTGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGGGCTTGGCGCCCTGCt                                          <  2:3056761/93‑1 (MQ=255)

                                                aCTCCGCGACAAATCATGATGGTCTTTCTGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGAAGCGGCGCTTGGCGCCCTGCtg                                         >  2:1208422/1‑92 (MQ=255)

                                                aCTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCtt                                         >  1:8042889/1‑93 (MQ=255)

                                                aCTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCtt                                         <  1:4982201/93‑1 (MQ=255)

                                                 cTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTg                                        <  2:4442469/93‑1 (MQ=255)

                                                  tCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgc                                       >  2:2932259/1‑93 (MQ=255)

                                                  tCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgc                                       <  2:165896/93‑1 (MQ=255)

                                                  tCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTCGGCGCCCTGCTTgc                                       >  1:5743797/1‑93 (MQ=255)

                                                   ccGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgcc                                      <  2:8122442/93‑1 (MQ=255)

                                                   ccGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgcc                                      >  2:1907083/1‑93 (MQ=255)

                                                    cgcgACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgcca                                     <  1:1272743/93‑1 (MQ=255)

                                                     gcgACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgccag                                    >  2:6690961/1‑93 (MQ=255)

                                                     gcgACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgccac                                    >  1:5300195/1‑92 (MQ=255)

                                                      cgACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgccagc                                   >  2:6209559/1‑93 (MQ=255)

                                                       gACAAATCATGGTGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgccagcc                                  >  2:8689217/1‑93 (MQ=255)

                                                        aCAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTgccagcca                                 >  1:5446181/1‑93 (MQ=255)

                                                         cAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGccagccag                                >  1:7614094/1‑93 (MQ=255)

                                                         cAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGccagccag                                <  2:4643267/93‑1 (MQ=255)

                                                           aaTCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGtt                              <  2:3552985/93‑1 (MQ=255)

                                                            aTCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTa                             <  1:8384606/93‑1 (MQ=255)

                                                             tCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTaa                            >  2:1095865/1‑93 (MQ=255)

                                                             tCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTaa                            >  1:6005475/1‑93 (MQ=255)

                                                             tCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTaa                            >  1:2281221/1‑93 (MQ=255)

                                                             tCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTaa                            >  2:3973801/1‑93 (MQ=255)

                                                               atgatgGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAaat                          <  2:2271863/93‑1 (MQ=255)

                                                                tgatgGTTTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGTCGCCCTGCTTGCCAGCCAGTTAaata                         <  1:6258778/93‑1 (MQ=255)

                                                                 gatgGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAaataa                        >  1:3882405/1‑93 (MQ=255)

                                                                 gatgGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAaataa                        <  2:7400878/93‑1 (MQ=255)

                                                                  atgGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAaataat                       <  1:1828654/93‑1 (MQ=255)

                                                                   tgGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATc                      >  1:1024173/1‑93 (MQ=255)

                                                                   tgGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATc                      <  2:7382145/93‑1 (MQ=255)

                                                                     gTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCta                    >  1:6749886/1‑92 (MQ=255)

                                                                       cTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGAt                  <  1:6512489/93‑1 (MQ=255)

                                                                       cTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGAt                  <  1:7750407/93‑1 (MQ=255)

                                                                       cTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGATGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGAt                  <  2:5602099/93‑1 (MQ=255)

                                                                        tttATGGGGCAAGGGGCCGGCGCCGGGATTCTCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATg                 <  1:661578/93‑1 (MQ=255)

                                                                         ttATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGc                <  1:404751/93‑1 (MQ=255)

                                                                         ttATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGc                <  2:3897365/93‑1 (MQ=255)

                                                                           aTGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCg              <  1:4112411/93‑1 (MQ=255)

                                                                             ggTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGAt            >  2:4673406/1‑93 (MQ=255)

                                                                              gTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATa           <  2:5358128/93‑1 (MQ=255)

                                                                              gTGCAAGGGGCCGCCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATa           <  2:327709/93‑1 (MQ=255)

                                                                               tGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATaa          <  2:4545526/93‑1 (MQ=255)

                                                                                gCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAAt         <  1:5958318/93‑1 (MQ=255)

                                                                                 cAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATc        <  1:3170425/93‑1 (MQ=255)

                                                                                 cAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATc        <  2:4995054/93‑1 (MQ=255)

                                                                                 cAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATc        <  1:7206205/93‑1 (MQ=255)

                                                                                  aaGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGTGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCg       >  2:4361087/1‑93 (MQ=255)

                                                                                   aGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCgg      >  1:4421084/1‑93 (MQ=255)

                                                                                   aGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCgg      <  1:549623/93‑1 (MQ=255)

                                                                                   aGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCgg      >  1:2549666/1‑93 (MQ=255)

                                                                                    ggggCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGc     >  2:6970275/1‑93 (MQ=255)

                                                                                    ggggCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGc     >  1:3745003/1‑93 (MQ=255)

                                                                                       gccggcgccggcATTATCGGTGCGATCCTCGGAGCGGAGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTc  <  2:378729/93‑1 (MQ=255)

GTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTC  >  NC_000913/1175517‑1175696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTG  >  NC_000913/1175514‑1175699

ATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCG                                                                                              >  SRR4302541.1245591/1‑94 (MQ=60)

 TGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGG                                                                                             <  SRR4302541.2114726/94‑1 (MQ=60)

  GGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGC                                                                                            >  SRR4302541.3572607/1‑94 (MQ=60)

   GTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCG                                                                                           >  SRR4302541.6618703/1‑94 (MQ=60)

    TAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGC                                                                                          >  SRR4302541.3202252/1‑94 (MQ=60)

     AATGGAGAAGCAGGGCGAAGTAGCGATCCTGCACACGCAAGGCTTAACTCCGCGACCAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCC                                                                                         >  SRR4302541.5280199/1‑94 (MQ=60)

     AATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCC                                                                                         >  SRR4302541.104289/1‑94 (MQ=60)

     AATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCC                                                                                         >  SRR4302541.5280202/1‑94 (MQ=60)

     AATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCC                                                                                         >  SRR4302541.2752883/1‑94 (MQ=60)

     AATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCC                                                                                         <  SRR4302541.1783655/94‑1 (MQ=60)

      ATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCG                                                                                        >  SRR4302541.5605848/1‑94 (MQ=60)

          AGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGAT                                                                                    >  SRR4302541.1395383/1‑94 (MQ=60)

            AAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTA                                                                                  >  SRR4302541.8237818/1‑94 (MQ=60)

            AAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTA                                                                                  >  SRR4302541.5666697/1‑94 (MQ=60)

            AAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTA                                                                                  >  SRR4302541.3118396/1‑94 (MQ=60)

             AGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTAT                                                                                 >  SRR4302541.5583052/1‑94 (MQ=60)

              GCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATC                                                                                <  SRR4302541.7132595/94‑1 (MQ=60)

               CAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCG                                                                               <  SRR4302541.8669102/94‑1 (MQ=60)

                AGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGG                                                                              >  SRR4302541.2051860/1‑94 (MQ=60)

                AGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGG                                                                              >  SRR4302541.4364893/1‑94 (MQ=60)

                AGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGAGCAAGGGGCCGGCGACGGGATTATCGG                                                                              >  SRR4302541.4364892/1‑94 (MQ=60)

                 GGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGT                                                                             <  SRR4302541.1176084/94‑1 (MQ=60)

                 GGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGT                                                                             <  SRR4302541.8811123/94‑1 (MQ=60)

                 GGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGT                                                                             <  SRR4302541.6059734/94‑1 (MQ=60)

                   GCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGC                                                                           >  SRR4302541.3507755/1‑94 (MQ=60)

                    CGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCG                                                                          >  SRR4302541.529989/1‑94 (MQ=60)

                    CGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCG                                                                          <  SRR4302541.3368949/94‑1 (MQ=60)

                     GAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGA                                                                         <  SRR4302541.7497244/94‑1 (MQ=60)

                       AGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATC                                                                       >  SRR4302541.7392103/1‑94 (MQ=60)

                       AGTAGCAATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGGGATC                                                                       >  SRR4302541.6845651/1‑94 (MQ=60)

                        GTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCC                                                                      >  SRR4302541.8032335/1‑94 (MQ=60)

                         TAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCT                                                                     <  SRR4302541.4348019/94‑1 (MQ=60)

                          AGCTATCCTGCAAACGCAAGGCTGAACTCCGCGACAAATCATGATGGTCTTTAGGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTC                                                                    <  SRR4302541.6314900/94‑1 (MQ=60)

                          AGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTC                                                                    <  SRR4302541.6314899/94‑1 (MQ=60)

                          AGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTC                                                                    >  SRR4302541.2731248/1‑94 (MQ=60)

                          AGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTC                                                                    <  SRR4302541.1738918/94‑1 (MQ=60)

                          AGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTC                                                                    >  SRR4302541.1787059/1‑94 (MQ=60)

                           GCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCG                                                                   <  SRR4302541.1608775/94‑1 (MQ=60)

                             GATCCTGCAAACGCAAGGCTTACCTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGA                                                                 <  SRR4302541.8847420/94‑1 (MQ=60)

                             GATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGA                                                                 >  SRR4302541.2151445/1‑94 (MQ=60)

                              ATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAG                                                                <  SRR4302541.3582543/94‑1 (MQ=60)

                               TCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGC                                                               >  SRR4302541.3836706/1‑94 (MQ=60)

                                CCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCG                                                              <  SRR4302541.7804383/94‑1 (MQ=60)

                                 CTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGG                                                             <  SRR4302541.4874295/94‑1 (MQ=60)

                                 CTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGG                                                             >  SRR4302541.5381163/1‑94 (MQ=60)

                                 CTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGG                                                             >  SRR4302541.900454/1‑94 (MQ=60)

                                  TGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGTCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGC                                                            >  SRR4302541.654755/1‑94 (MQ=60)

                                     AAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGcggct                                                         >  SRR4302541.2902119/1‑89 (MQ=60)

                                     AAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGAGATCCTCGGAGCGGCGCT                                                         >  SRR4302541.1752811/1‑94 (MQ=60)

                                      AACGCAAGGCTTAACTCCGCGACGAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTT                                                        >  SRR4302541.4291824/1‑94 (MQ=60)

                                      AACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTT                                                        >  SRR4302541.1106023/1‑94 (MQ=60)

                                      AACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTT                                                        <  SRR4302541.6475082/94‑1 (MQ=60)

                                      AACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTT                                                        <  SRR4302541.7134267/94‑1 (MQ=60)

                                      AACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTT                                                        >  SRR4302541.7420929/1‑94 (MQ=60)

                                        CGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGG                                                      >  SRR4302541.1162100/1‑94 (MQ=60)

                                          CAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCG                                                    >  SRR4302541.3664332/1‑94 (MQ=60)

                                          CAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCG                                                    <  SRR4302541.2143548/94‑1 (MQ=60)

                                          CAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCG                                                    >  SRR4302541.4034565/1‑94 (MQ=60)

                                          CAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCG                                                    <  SRR4302541.4550673/94‑1 (MQ=60)

                                          CAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGAGATCCTCGGAGAGGCGCTTCGCG                                                    >  SRR4302541.789157/1‑94 (MQ=60)

                                           AAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGC                                                   >  SRR4302541.95547/1‑94 (MQ=60)

                                           AAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGC                                                   >  SRR4302541.821347/1‑94 (MQ=60)

                                            AGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCC                                                  >  SRR4302541.7002120/1‑94 (MQ=60)

                                            AGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCC                                                  >  SRR4302541.138128/1‑94 (MQ=60)

                                             GGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCC                                                 >  SRR4302541.2172072/1‑94 (MQ=60)

                                              GCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCT                                                <  SRR4302541.2321685/94‑1 (MQ=60)

                                              GCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCT                                                <  SRR4302541.3260663/94‑1 (MQ=60)

                                              GCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCT                                                <  SRR4302541.8873009/94‑1 (MQ=60)

                                               CTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTG                                               >  SRR4302541.1200821/1‑94 (MQ=60)

                                               CTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTG                                               >  SRR4302541.448581/1‑94 (MQ=60)

                                               CTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTG                                               >  SRR4302541.1200822/1‑94 (MQ=60)

                                                 TAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCT                                             <  SRR4302541.6776184/94‑1 (MQ=60)

                                                 TAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCT                                             <  SRR4302541.1777119/94‑1 (MQ=60)

                                                  AACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTT                                            <  SRR4302541.1636212/94‑1 (MQ=60)

                                                  AACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTT                                            >  SRR4302541.4319737/1‑94 (MQ=60)

                                                  AACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTT                                            >  SRR4302541.8131331/1‑94 (MQ=60)

                                                  AACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGAGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTT                                            >  SRR4302541.7117068/1‑94 (MQ=60)

                                                   ACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTG                                           <  SRR4302541.5033302/94‑1 (MQ=60)

                                                    CTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTCGGCGCCCTGCTTGC                                          >  SRR4302541.5802307/1‑94 (MQ=60)

                                                     TCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCC                                         >  SRR4302541.3000418/1‑94 (MQ=60)

                                                      CCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCA                                        <  SRR4302541.7035003/94‑1 (MQ=60)

                                                       CGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAG                                       <  SRR4302541.1285394/94‑1 (MQ=60)

                                                       CGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAC                                       >  SRR4302541.5354751/1‑94 (MQ=60)

                                                         CGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCC                                     <  SRR4302541.4127585/94‑1 (MQ=60)

                                                          GACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCA                                    >  SRR4302541.5502061/1‑94 (MQ=60)

                                                          GACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCA                                    >  SRR4302541.4985514/1‑94 (MQ=60)

                                                           ACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAG                                   <  SRR4302541.6350949/94‑1 (MQ=60)

                                                           ACAAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAG                                   >  SRR4302541.7695440/1‑94 (MQ=60)

                                                             AAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTT                                 >  SRR4302541.5516523/1‑94 (MQ=60)

                                                             AAATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTT                                 <  SRR4302541.824890/94‑1 (MQ=60)

                                                               ATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAA                               >  SRR4302541.2303273/1‑94 (MQ=60)

                                                               ATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAA                               <  SRR4302541.8475408/94‑1 (MQ=60)

                                                               ATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAA                               >  SRR4302541.6067008/1‑94 (MQ=60)

                                                               ATCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAA                               <  SRR4302541.5470703/94‑1 (MQ=60)

                                                                TCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAA                              >  SRR4302541.7745748/1‑94 (MQ=60)

                                                                TCATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAA                              <  SRR4302541.8917270/94‑1 (MQ=60)

                                                                  ATGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATA                            <  SRR4302541.4151474/94‑1 (MQ=60)

                                                                   TGATGGTTTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGTCGCCCTGCTTGCCAGCCAGTTAAATAA                           <  SRR4302541.6322706/94‑1 (MQ=60)

                                                                   TGATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAA                           >  SRR4302541.3921380/1‑94 (MQ=60)

                                                                     ATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATC                         <  SRR4302541.1846393/94‑1 (MQ=60)

                                                                     ATGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATC                         >  SRR4302541.1033630/1‑94 (MQ=60)

                                                                      TGGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCT                        <  SRR4302541.1656407/94‑1 (MQ=60)

                                                                       GGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTG                       <  SRR4302541.7499633/94‑1 (MQ=60)

                                                                       GGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTG                       >  SRR4302541.2435350/1‑94 (MQ=60)

                                                                       GGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTG                       >  SRR4302541.6518186/1‑94 (MQ=60)

                                                                       GGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTG                       <  SRR4302541.340108/94‑1 (MQ=60)

                                                                       GGTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTA                       >  SRR4302541.6822040/1‑94 (MQ=60)

                                                                        GTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGA                      >  SRR4302541.5826405/1‑94 (MQ=60)

                                                                        GTCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGA                      >  SRR4302541.5137968/1‑94 (MQ=60)

                                                                         TCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGAT                     <  SRR4302541.3293930/94‑1 (MQ=60)

                                                                         GCTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGAT                     <  SRR4302541.1787661/94‑1 (MQ=60)

                                                                          CTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATG                    >  SRR4302541.3846268/1‑94 (MQ=60)

                                                                          CTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATG                    <  SRR4302541.5672071/94‑1 (MQ=60)

                                                                          CTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATG                    <  SRR4302541.7832868/94‑1 (MQ=60)

                                                                          CTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATG                    <  SRR4302541.8323423/94‑1 (MQ=60)

                                                                          CTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATG                    >  SRR4302541.125913/1‑94 (MQ=60)

                                                                          CTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATG                    <  SRR4302541.6362074/94‑1 (MQ=60)

                                                                          CTTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATG                    <  SRR4302541.6578942/94‑1 (MQ=60)

                                                                          ATTTATGGTGCAAGGGGCCGGCGCCGGGCTTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTCCCAGCCAGTTAAATAATCTGATG                    <  SRR4302541.6362075/94‑1 (MQ=60)

                                                                           TTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGC                   >  SRR4302541.1576384/1‑94 (MQ=60)

                                                                           TTTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGC                   >  SRR4302541.7413393/1‑94 (MQ=60)

                                                                           TTTATGGGGCAAGGGGCCGGCGCCGGGATTCTCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGC                   <  SRR4302541.667821/94‑1 (MQ=60)

                                                                            TTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCC                  <  SRR4302541.408501/94‑1 (MQ=60)

                                                                            TTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCC                  >  SRR4302541.6682383/1‑94 (MQ=60)

                                                                            TTATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCC                  <  SRR4302541.7411923/94‑1 (MQ=60)

                                                                              ATGGTGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGA                <  SRR4302541.4153031/94‑1 (MQ=60)

                                                                                  TGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAAT            <  SRR4302541.6913226/94‑1 (MQ=60)

                                                                                  TGCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGACGCCGATAAT            >  SRR4302541.5255151/1‑94 (MQ=60)

                                                                                   GCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATC           >  SRR4302541.7294044/1‑94 (MQ=60)

                                                                                   GCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATC           <  SRR4302541.269077/94‑1 (MQ=60)

                                                                                   GCAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATC           <  SRR4302541.6019049/94‑1 (MQ=60)

                                                                                    CAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCG          <  SRR4302541.5801141/94‑1 (MQ=60)

                                                                                    CAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCG          <  SRR4302541.3202453/94‑1 (MQ=60)

                                                                                    CAAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCG          <  SRR4302541.7283078/94‑1 (MQ=60)

                                                                                     AAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGG         >  SRR4302541.2575239/1‑94 (MQ=60)

                                                                                     AAGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGG         >  SRR4302541.4465464/1‑94 (MQ=60)

                                                                                      AGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGC        >  SRR4302541.3782692/1‑94 (MQ=60)

                                                                                      AGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGC        >  SRR4302541.7272066/1‑94 (MQ=60)

                                                                                      AGGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGC        <  SRR4302541.554801/94‑1 (MQ=60)

                                                                                       GGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCTCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCG       <  SRR4302541.3084358/94‑1 (MQ=60)

                                                                                       GGGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCG       <  SRR4302541.1325317/94‑1 (MQ=60)

                                                                                        GGGCCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGT      <  SRR4302541.5960697/94‑1 (MQ=60)

                                                                                           CCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCT   <  SRR4302541.2110294/94‑1 (MQ=60)

                                                                                           CCGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCT   >  SRR4302541.5983596/1‑94 (MQ=60)

                                                                                            CGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTG  >  SRR4302541.5353370/1‑94 (MQ=60)

                                                                                            CGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTG  <  SRR4302541.2610544/94‑1 (MQ=60)

                                                                                            CGGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTG  >  SRR4302541.7457790/1‑94 (MQ=60)

ATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTG  >  NC_000913/1175514‑1175699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`