Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,238,083 A→G A430A (GCA→GCG)  dadA → D‑amino acid dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,238,0830AG100.0% 786.0 / NA 225A430A (GCA→GCG) dadAD‑amino acid dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (113/112);  total (113/112)

Only 100 of 248 total aligned reads displayed.

TCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGG  >  NC_000913/1237993‑1238171
                                                                                          |                                                                                        
tCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgca                                                                                        >  2:5281500/1‑93 (MQ=255)
  gCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcaca                                                                                      <  2:729112/93‑1 (MQ=255)
  gCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcaca                                                                                      >  2:1711821/1‑93 (MQ=255)
  gCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcaca                                                                                      <  1:2323671/93‑1 (MQ=255)
   cACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcacac                                                                                     >  2:3035723/1‑92 (MQ=255)
    ccGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                    <  2:2487556/92‑1 (MQ=255)
    aCGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                    <  2:5089127/93‑1 (MQ=255)
     cGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCt                                                                                   <  2:79612/93‑1 (MQ=255)
     cGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCt                                                                                   <  2:1142547/93‑1 (MQ=255)
      gCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTa                                                                                  >  2:5573591/1‑93 (MQ=255)
       ccAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTaa                                                                                 >  1:4300217/1‑93 (MQ=255)
       ccAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTaa                                                                                 <  1:1504224/93‑1 (MQ=255)
         aGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAgg                                                                               <  2:4442653/93‑1 (MQ=255)
         aGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAgg                                                                               >  1:6451617/1‑93 (MQ=255)
         aGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAgg                                                                               <  1:408606/93‑1 (MQ=255)
          gCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGa                                                                              >  2:3820593/1‑93 (MQ=255)
            gATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGaaa                                                                            >  2:1800713/1‑93 (MQ=255)
              tCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACg                                                                          >  1:3373500/1‑93 (MQ=255)
               cccATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACga                                                                         >  1:2450079/1‑93 (MQ=255)
               cccATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACga                                                                         >  1:4791312/1‑93 (MQ=255)
                ccATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACgag                                                                        <  2:4993833/93‑1 (MQ=255)
                ccATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACgag                                                                        >  1:1735336/1‑93 (MQ=255)
                 cATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACgaga                                                                       <  2:394928/93‑1 (MQ=255)
                  atatGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGAt                                                                      <  2:214379/93‑1 (MQ=255)
                  atatGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGAt                                                                      <  2:6094593/93‑1 (MQ=255)
                   tatGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATg                                                                     <  1:2102434/93‑1 (MQ=255)
                    atGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGa                                                                    <  1:988549/93‑1 (MQ=255)
                     tGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAc                                                                   >  2:3265043/1‑93 (MQ=255)
                      gAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAcc                                                                  <  1:3583333/93‑1 (MQ=255)
                      gAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAcc                                                                  >  2:1115482/1‑93 (MQ=255)
                      gAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAcc                                                                  <  1:5076256/93‑1 (MQ=255)
                       aGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAccc                                                                 <  1:4626974/93‑1 (MQ=255)
                       aGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAccc                                                                 >  2:4197560/1‑93 (MQ=255)
                        ggATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCg                                                                >  2:4881197/1‑93 (MQ=255)
                          aTCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTc                                                              >  1:4534377/1‑93 (MQ=255)
                           tCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTcc                                                             >  2:2650053/1‑93 (MQ=255)
                           tCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATCGCGCGCACAGCTAAGGAAACGAGATGACCCGTcc                                                             >  1:965/1‑93 (MQ=255)
                             tAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGa                                                           <  2:1978751/93‑1 (MQ=255)
                              aaGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGAt                                                          >  2:5617136/1‑93 (MQ=255)
                              aaGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGAt                                                          >  2:644962/1‑93 (MQ=255)
                               aGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGTCATTTACATGGCGCGCACAGCTCAGGAAACGAGATGACCCGTCCGATa                                                         >  1:2203742/1‑93 (MQ=255)
                               aGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATTGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGAtc                                                         >  1:2810101/1‑92 (MQ=255)
                               aGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATa                                                         >  1:5281075/1‑93 (MQ=255)
                               aGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATa                                                         >  1:3862664/1‑93 (MQ=255)
                                  gTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAg                                                      >  2:5188686/1‑93 (MQ=255)
                                  gTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAg                                                      <  1:2242358/93‑1 (MQ=255)
                                    aGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGc                                                    <  2:5831222/93‑1 (MQ=255)
                                     gcgcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGcc                                                   >  1:2652184/1‑93 (MQ=255)
                                      cgcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATAGAGGCCa                                                  <  1:4939749/93‑1 (MQ=255)
                                      cgcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCa                                                  <  1:5140803/93‑1 (MQ=255)
                                       gcgcTACAGCCGTGGATTTACGCCATCACGTCCGAGCCATTTACATCGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAg                                                 >  1:1251145/1‑93 (MQ=255)
                                           tACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTc                                             <  2:3079908/93‑1 (MQ=255)
                                            aCAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCg                                            <  2:4908484/93‑1 (MQ=255)
                                            aCAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCg                                            <  2:5315479/93‑1 (MQ=255)
                                             cAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGa                                           >  1:5376856/1‑93 (MQ=255)
                                               gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGTCCCGTCTGATACCGGCCAGCCTCGATc                                         >  2:3623734/1‑93 (MQ=255)
                                               gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                         <  1:1688488/93‑1 (MQ=255)
                                               gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                         >  2:3321043/1‑93 (MQ=255)
                                               gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                         <  1:4222179/93‑1 (MQ=255)
                                               gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                         >  2:578331/1‑93 (MQ=255)
                                               gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                         <  1:1163270/93‑1 (MQ=255)
                                                  gTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGc                                      >  2:6468921/1‑93 (MQ=255)
                                                   tGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCa                                     <  2:5887256/93‑1 (MQ=255)
                                                    ggATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAg                                    <  1:5636306/93‑1 (MQ=255)
                                                    ggATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAg                                    >  2:5455248/1‑93 (MQ=255)
                                                    ggATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGCCCAGCCTCGATCTGCAg                                    >  2:4253575/1‑93 (MQ=255)
                                                     gATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAgg                                   >  1:4909043/1‑93 (MQ=255)
                                                     gATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAgg                                   <  1:1780212/93‑1 (MQ=255)
                                                      aTTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGc                                  <  1:1813332/93‑1 (MQ=255)
                                                             ccATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTaaaa                           <  2:4222762/93‑1 (MQ=255)
                                                              cATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAAc                          >  1:4683686/1‑93 (MQ=255)
                                                              cATCACGTCCGGCCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAAc                          <  2:3866837/93‑1 (MQ=255)
                                                               aTCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACa                         >  1:5426963/1‑93 (MQ=255)
                                                                tCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAg                        <  2:4665961/93‑1 (MQ=255)
                                                                tCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAg                        >  2:1600047/1‑93 (MQ=255)
                                                                  aCGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGaa                      >  2:2926802/1‑93 (MQ=255)
                                                                   cGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAAt                     >  1:6195309/1‑93 (MQ=255)
                                                                    gTCCGGGCCATCTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATc                    <  2:3394187/93‑1 (MQ=255)
                                                                     tcggggCCATTTACAGGGCGCGCCCAGCTAAGGAACCGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCt                   <  1:3988527/90‑1 (MQ=255)
                                                                     tCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCt                   >  1:1037575/1‑93 (MQ=255)
                                                                     tCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCt                   <  1:2853876/93‑1 (MQ=255)
                                                                       cGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATCCAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGt                 <  1:1348339/93‑1 (MQ=255)
                                                                        gggCCATTTACATGGCGCGCACAGCTAAGGAAACAAGATGTCCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTc                <  1:702002/93‑1 (MQ=255)
                                                                         ggCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTcc               <  1:5786619/93‑1 (MQ=255)
                                                                         ggCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTcc               >  2:4425387/1‑93 (MQ=255)
                                                                         ggCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTcc               >  1:2578811/1‑93 (MQ=255)
                                                                         ggCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTcc               <  2:6000032/93‑1 (MQ=255)
                                                                          gCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCa              <  1:6233087/93‑1 (MQ=255)
                                                                          gCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCa              >  1:5302580/1‑93 (MQ=255)
                                                                           ccATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAt             >  1:903710/1‑93 (MQ=255)
                                                                            cATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAtt            <  2:2507219/93‑1 (MQ=255)
                                                                            cATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAtt            <  2:2856174/93‑1 (MQ=255)
                                                                             aTTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTg           <  1:3221610/93‑1 (MQ=255)
                                                                              tttACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGt          >  1:554301/1‑93 (MQ=255)
                                                                              ttcacacGGCGCGCACAGCTANGGAAACGAGATGACCCGTCCGATACAGCCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGt          <  2:2767230/90‑1 (MQ=255)
                                                                                 agaTGGCGCGCACAGCTAAGGAAACGAGATGCCCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCg       <  1:272730/91‑1 (MQ=255)
                                                                                   aTGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGcc     >  2:1475153/1‑93 (MQ=255)
                                                                                   aTGGCGCGCACAGCTAAGCAACCGAGATGACGCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGcc     <  1:3139736/93‑1 (MQ=255)
                                                                                    tGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCa    <  2:4096604/93‑1 (MQ=255)
                                                                                     ggCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAg   >  1:5739813/1‑93 (MQ=255)
                                                                                          |                                                                                        
TCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGG  >  NC_000913/1237993‑1238171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCG  >  NC_000913/1237990‑1238176
                                                                                             |                                                                                             
TGGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCG                                                                                               >  SRR4302534.5906748/1‑94 (MQ=60)
TGGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCG                                                                                               <  SRR4302534.5448299/94‑1 (MQ=60)
TGGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCG                                                                                               >  SRR4302534.5711682/1‑94 (MQ=60)
 GGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGC                                                                                              >  SRR4302534.3151401/1‑94 (MQ=60)
 GGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGC                                                                                              >  SRR4302534.4812576/1‑94 (MQ=60)
 GGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGC                                                                                              >  SRR4302534.5246390/1‑94 (MQ=60)
   TCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCAC                                                                                            <  SRR4302534.979629/94‑1 (MQ=60)
   TCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGAGCCATTTACATGGCGCGCAC                                                                                            <  SRR4302534.5157585/94‑1 (MQ=60)
    CGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACA                                                                                           <  SRR4302534.4609698/94‑1 (MQ=60)
    CGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACA                                                                                           <  SRR4302534.563860/94‑1 (MQ=60)
     GCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAG                                                                                          <  SRR4302534.2385340/94‑1 (MQ=60)
      CACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGC                                                                                         <  SRR4302534.5711054/94‑1 (MQ=60)
       ACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCT                                                                                        >  SRR4302534.2837070/1‑94 (MQ=60)
         GCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAA                                                                                      >  SRR4302534.4416764/1‑94 (MQ=60)
          CCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAG                                                                                     <  SRR4302534.901133/94‑1 (MQ=60)
          CCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAG                                                                                     <  SRR4302534.1543797/94‑1 (MQ=60)
           CAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGG                                                                                    >  SRR4302534.6625470/1‑94 (MQ=60)
           CAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGG                                                                                    >  SRR4302534.4996695/1‑94 (MQ=60)
            AGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATTGCGCGCACAGCTAAGGA                                                                                   <  SRR4302534.5892753/94‑1 (MQ=60)
            AGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGA                                                                                   <  SRR4302534.419049/94‑1 (MQ=60)
            AGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGA                                                                                   >  SRR4302534.6395292/1‑94 (MQ=60)
               GATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAAC                                                                                <  SRR4302534.1783182/94‑1 (MQ=60)
               GATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAAC                                                                                >  SRR4302534.2335375/1‑94 (MQ=60)
                ATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACG                                                                               >  SRR4302534.1225249/1‑94 (MQ=60)
                ATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACG                                                                               >  SRR4302534.3463898/1‑94 (MQ=60)
                 TCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGA                                                                              >  SRR4302534.4921409/1‑94 (MQ=60)
                 TCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGA                                                                              >  SRR4302534.2515176/1‑94 (MQ=60)
                  CCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAG                                                                             >  SRR4302534.5905528/1‑94 (MQ=60)
                  CCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAG                                                                             >  SRR4302534.1780402/1‑94 (MQ=60)
                   CCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGA                                                                            >  SRR4302534.1444652/1‑94 (MQ=60)
                   CCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGA                                                                            >  SRR4302534.3092566/1‑94 (MQ=60)
                     ATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATG                                                                          >  SRR4302534.2721783/1‑94 (MQ=60)
                      TATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGA                                                                         <  SRR4302534.2157624/94‑1 (MQ=60)
                      TATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGA                                                                         <  SRR4302534.3575509/94‑1 (MQ=60)
                       ATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAC                                                                        <  SRR4302534.1013829/94‑1 (MQ=60)
                         GAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCC                                                                      <  SRR4302534.5213293/94‑1 (MQ=60)
                         GAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCC                                                                      <  SRR4302534.1808742/94‑1 (MQ=60)
                         GAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCC                                                                      >  SRR4302534.2548457/1‑94 (MQ=60)
                         GAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCC                                                                      <  SRR4302534.3679723/94‑1 (MQ=60)
                          AGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCG                                                                     >  SRR4302534.2772564/1‑94 (MQ=60)
                          AGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCG                                                                     <  SRR4302534.4752517/94‑1 (MQ=60)
                           GGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACGCGT                                                                    >  SRR4302534.5069726/1‑94 (MQ=60)
                            GATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTC                                                                   >  SRR4302534.4657249/1‑94 (MQ=60)
                            GATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTC                                                                   >  SRR4302534.5131160/1‑94 (MQ=60)
                            GATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTC                                                                   >  SRR4302534.5457893/1‑94 (MQ=60)
                             ATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATCGCGCGCACAGCTAAGGAAACGAGATGACCCGTCC                                                                  >  SRR4302534.987/1‑94 (MQ=60)
                              TCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCG                                                                 >  SRR4302534.806235/1‑94 (MQ=60)
                               CTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGA                                                                >  SRR4302534.345380/1‑94 (MQ=60)
                                TAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGAT                                                               <  SRR4302534.2176059/94‑1 (MQ=60)
                                TAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGCGGCATTTACATGGCGCGCACAGCTAAGGAAACGAGCAGACCCGTCCGAT                                                               >  SRR4302534.5860936/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGTCATTTACATGGCGCGCACAGCTCAGGAAACGAGATGACCCGTCCGATA                                                              >  SRR4302534.2262056/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATTGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATC                                                              >  SRR4302534.2883984/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              >  SRR4302534.2280337/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              >  SRR4302534.5423670/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              >  SRR4302534.4168409/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              >  SRR4302534.3966376/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              <  SRR4302534.370399/94‑1 (MQ=60)
                                   GCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACA                                                            <  SRR4302534.4864448/94‑1 (MQ=60)
                                    CGTAGCGCGCTACAGCCGTGGATTTATGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAG                                                           <  SRR4302534.5341994/94‑1 (MQ=60)
                                    CGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAG                                                           <  SRR4302534.1888794/94‑1 (MQ=60)
                                     GTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGG                                                          <  SRR4302534.2301778/94‑1 (MQ=60)
                                     GTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGG                                                          >  SRR4302534.2293003/1‑94 (MQ=60)
                                       AGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCC                                                        >  SRR4302534.2722324/1‑94 (MQ=60)
                                        GCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCA                                                       <  SRR4302534.161169/94‑1 (MQ=60)
                                         CGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATAGAGGCCAG                                                      <  SRR4302534.5073595/94‑1 (MQ=60)
                                         CGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAG                                                      <  SRR4302534.5279548/94‑1 (MQ=60)
                                         CGCGCTACAGCCGTGGATTTACGCCATCACGTCCGAGCCATTTACATCGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAG                                                      >  SRR4302534.1284121/1‑94 (MQ=60)
                                          GCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGC                                                     <  SRR4302534.5501881/94‑1 (MQ=60)
                                            GCTACAGCCGTGTATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGCCCCGTCCGATACAGGCCAGCCT                                                   <  SRR4302534.5633927/94‑1 (MQ=60)
                                             CTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTC                                                  <  SRR4302534.4035666/94‑1 (MQ=60)
                                              TACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGCCCCGTCCGATACAGGCCAGCCTCG                                                 <  SRR4302534.3251890/94‑1 (MQ=60)
                                              TACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCG                                                 >  SRR4302534.4219040/1‑94 (MQ=60)
                                               ACAGCCGTGGATTTACGCCCTCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGA                                                >  SRR4302534.3324114/1‑94 (MQ=60)
                                               ACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGA                                                >  SRR4302534.5521729/1‑94 (MQ=60)
                                                CAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGAT                                               <  SRR4302534.1130032/94‑1 (MQ=60)
                                                CAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGAT                                               <  SRR4302534.2448912/94‑1 (MQ=60)
                                                  GCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCT                                             <  SRR4302534.4372062/94‑1 (MQ=60)
                                                  GCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCT                                             <  SRR4302534.1193802/94‑1 (MQ=60)
                                                  GCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCT                                             >  SRR4302534.5877148/1‑94 (MQ=60)
                                                  GCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCT                                             <  SRR4302534.1732389/94‑1 (MQ=60)
                                                  GCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCT                                             <  SRR4302534.4335998/94‑1 (MQ=60)
                                                  GCCGGGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCAAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCT                                             <  SRR4302534.5243859/94‑1 (MQ=60)
                                                   CCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTG                                            <  SRR4302534.4790161/94‑1 (MQ=60)
                                                       GGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGG                                        <  SRR4302534.5788863/94‑1 (MQ=60)
                                                       GGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGG                                        >  SRR4302534.5042098/1‑94 (MQ=60)
                                                       GGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGACCGCCTCGATCTGCAGG                                        >  SRR4302534.1302655/1‑94 (MQ=60)
                                                        GATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGC                                       <  SRR4302534.629640/94‑1 (MQ=60)
                                                        GATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGC                                       <  SRR4302534.1826437/94‑1 (MQ=60)
                                                         ATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCA                                      <  SRR4302534.1860438/94‑1 (MQ=60)
                                                            TACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTA                                   <  SRR4302534.2906889/94‑1 (MQ=60)
                                                            TACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTA                                   >  SRR4302534.4782461/1‑94 (MQ=60)
                                                             ACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAA                                  <  SRR4302534.6053570/94‑1 (MQ=60)
                                                                CCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAAC                               >  SRR4302534.4810844/1‑94 (MQ=60)
                                                                 CATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACA                              >  SRR4302534.5573039/1‑94 (MQ=60)
                                                                  ATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAG                             <  SRR4302534.2967540/94‑1 (MQ=60)
                                                                  ATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAG                             <  SRR4302534.553566/94‑1 (MQ=60)
                                                                     ACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAAT                          >  SRR4302534.6363875/1‑94 (MQ=60)
                                                                      CGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATC                         <  SRR4302534.1286833/94‑1 (MQ=60)
                                                                      CGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATC                         >  SRR4302534.3734373/1‑94 (MQ=60)
                                                                       GTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCT                        <  SRR4302534.1948944/94‑1 (MQ=60)
                                                                       GTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCT                        >  SRR4302534.1064352/1‑94 (MQ=60)
                                                                        TCGGGGCCATTTACAGGGCGCGCCCAGCTAAGGAACCGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTG                       <  SRR4302534.4095239/94‑1 (MQ=60)
                                                                        TCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCCGGCATTAAAACAGAATCTG                       >  SRR4302534.687482/1‑94 (MQ=60)
                                                                        TCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTG                       <  SRR4302534.4706154/94‑1 (MQ=60)
                                                                        TCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTG                       <  SRR4302534.2928840/94‑1 (MQ=60)
                                                                         CGGGGCTATTTACAGGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGT                      <  SRR4302534.6601862/94‑1 (MQ=60)
                                                                          CGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATCCAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTC                     <  SRR4302534.1383875/94‑1 (MQ=60)
                                                                           GGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCC                    >  SRR4302534.2647115/1‑94 (MQ=60)
                                                                           GGGCCATTTACATGGCGCGCACAGCTAAGGAAACAAGATGTCCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCC                    <  SRR4302534.719214/94‑1 (MQ=60)
                                                                            GGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCA                   <  SRR4302534.5943589/94‑1 (MQ=60)
                                                                            GGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCA                   <  SRR4302534.2420740/94‑1 (MQ=60)
                                                                            GGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCA                   >  SRR4302534.5445658/1‑94 (MQ=60)
                                                                            GGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCA                   >  SRR4302534.4986279/1‑94 (MQ=60)
                                                                             GCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAT                  >  SRR4302534.4624710/1‑94 (MQ=60)
                                                                             GCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAT                  <  SRR4302534.6402271/94‑1 (MQ=60)
                                                                             GCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAT                  >  SRR4302534.926658/1‑94 (MQ=60)
                                                                             GCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAT                  >  SRR4302534.1699690/1‑94 (MQ=60)
                                                                               CATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTG                >  SRR4302534.1859324/1‑94 (MQ=60)
                                                                                ATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGT               <  SRR4302534.1011327/94‑1 (MQ=60)
                                                                                ATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGT               <  SRR4302534.3307152/94‑1 (MQ=60)
                                                                                ATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGT               >  SRR4302534.2705294/1‑94 (MQ=60)
                                                                                ATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGT               >  SRR4302534.567966/1‑94 (MQ=60)
                                                                                ATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGT               <  SRR4302534.1756294/94‑1 (MQ=60)
                                                                                   TACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCG            >  SRR4302534.1006901/1‑94 (MQ=60)
                                                                                    AGATGGCGCGCACAGCTAAGGAAACGAGATGCCCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGC           <  SRR4302534.279793/94‑1 (MQ=60)
                                                                                     CATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCC          >  SRR4302534.2826817/1‑94 (MQ=60)
                                                                                      ATGGCGCGCACAGCTAAGCAACCGAGATGACGCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCA         <  SRR4302534.3222305/94‑1 (MQ=60)
                                                                                       TGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAG        >  SRR4302534.5895380/1‑94 (MQ=60)
                                                                                       TGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAG        <  SRR4302534.4106365/94‑1 (MQ=60)
                                                                                       TGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAG        <  SRR4302534.5437209/94‑1 (MQ=60)
                                                                                        GGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGG       <  SRR4302534.5317644/94‑1 (MQ=60)
                                                                                         GCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGC      <  SRR4302534.6604348/94‑1 (MQ=60)
                                                                                           GCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCG    >  SRR4302534.6599618/1‑94 (MQ=60)
                                                                                             GCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCG  <  SRR4302534.1100343/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCG  >  NC_000913/1237990‑1238176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑