Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,238,083 A→G A430A (GCA→GCG)  dadA → D‑amino acid dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,238,0830AG99.6% 773.6 / NA 224A430A (GCA→GCG) dadAD‑amino acid dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (115/108);  minor base C (0/1);  total (115/109)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGC  >  NC_000913/1237993‑1238172
                                                                                          |                                                                                         
gcGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgca                                                                                         <  2:7849294/92‑1 (MQ=255)
 cGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcac                                                                                        >  1:3454461/1‑93 (MQ=255)
  gCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcaca                                                                                       >  1:1763649/1‑93 (MQ=255)
   cACGCCAGCGATCCCATATGCGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGGCATTTACATGGCGCGCACAg                                                                                      >  1:7964261/1‑93 (MQ=255)
    aCGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                     >  1:824462/1‑93 (MQ=255)
    aCGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                     <  1:3789401/93‑1 (MQ=255)
    aCGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                     >  1:8537889/1‑93 (MQ=255)
    aCGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                     >  1:8223518/1‑93 (MQ=255)
    aCGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                     >  2:1399834/1‑93 (MQ=255)
     cGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCt                                                                                    >  1:8464953/1‑93 (MQ=255)
        cAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAg                                                                                 >  2:6979946/1‑93 (MQ=255)
         aGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAgg                                                                                <  2:7578243/93‑1 (MQ=255)
          gCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGa                                                                               >  1:1678262/1‑93 (MQ=255)
            gATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGaaa                                                                             <  2:7255767/93‑1 (MQ=255)
            gATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGaaa                                                                             >  1:5970881/1‑93 (MQ=255)
            gATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGaaa                                                                             <  2:31424/93‑1 (MQ=255)
             aTCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAAc                                                                            >  2:1229583/1‑93 (MQ=255)
             aTCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAAc                                                                            <  2:398325/93‑1 (MQ=255)
             aTCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAAc                                                                            <  1:2973527/93‑1 (MQ=255)
              tCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACg                                                                           >  2:1132332/1‑93 (MQ=255)
               cccATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACga                                                                          <  1:973669/93‑1 (MQ=255)
               cccATATGAGGATCTAAGCGTAGCGCGCTACAACCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACga                                                                          <  1:6300287/93‑1 (MQ=255)
                   tatGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATg                                                                      <  2:8350826/93‑1 (MQ=255)
                    atGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGa                                                                     <  2:5152762/93‑1 (MQ=255)
                     tGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAc                                                                    >  2:4906585/1‑93 (MQ=255)
                     tGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAc                                                                    <  2:2639292/93‑1 (MQ=255)
                     tGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAc                                                                    >  1:7376974/1‑93 (MQ=255)
                         gATCAAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGt                                                                >  2:5604232/1‑93 (MQ=255)
                           tCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGGCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTcc                                                              >  2:8471439/1‑93 (MQ=255)
                           tCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTcc                                                              <  2:1944669/93‑1 (MQ=255)
                             tAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGa                                                            >  1:253755/1‑93 (MQ=255)
                              aaGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGAt                                                           <  1:384485/93‑1 (MQ=255)
                              aaGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGAt                                                           <  1:4847800/93‑1 (MQ=255)
                                 cGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACa                                                        <  1:5021739/93‑1 (MQ=255)
                                  gTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAg                                                       <  1:2682817/93‑1 (MQ=255)
                                    aGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGc                                                     <  2:1821770/93‑1 (MQ=255)
                                     gcgcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGca                                                    >  1:1327874/1‑92 (MQ=255)
                                      cgcgcTACAGCCGTGGATTTACCCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCa                                                   <  2:6036790/93‑1 (MQ=255)
                                       gcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAg                                                  <  1:4073702/93‑1 (MQ=255)
                                         gcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGcc                                                <  2:8283682/93‑1 (MQ=255)
                                          cTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCt                                               >  1:704426/1‑93 (MQ=255)
                                          cTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCt                                               <  1:5592596/93‑1 (MQ=255)
                                          cTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCt                                               >  2:4532197/1‑93 (MQ=255)
                                          cTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCt                                               <  1:1232298/93‑1 (MQ=255)
                                           tACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTc                                              >  2:349898/1‑93 (MQ=255)
                                           tACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTc                                              <  2:1673763/93‑1 (MQ=255)
                                           tACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTc                                              >  2:6147140/1‑93 (MQ=255)
                                           tACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTc                                              >  2:802807/1‑93 (MQ=255)
                                           tACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTc                                              <  1:2860483/93‑1 (MQ=255)
                                           ctcAGCCGTCGATTTACGCCACCACGTCCGGGCCATTTACATGGCGCCCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTc                                              <  1:6612667/91‑1 (MQ=255)
                                            aCAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCg                                             <  1:4284126/93‑1 (MQ=255)
                                              aGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGAt                                           <  1:7795460/93‑1 (MQ=255)
                                              aGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGAt                                           <  2:2383892/93‑1 (MQ=255)
                                              aGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGAt                                           >  2:363174/1‑93 (MQ=255)
                                               gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                          <  2:5656155/93‑1 (MQ=255)
                                                ccGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCt                                         <  1:232734/93‑1 (MQ=255)
                                                ccGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCt                                         <  2:2250112/93‑1 (MQ=255)
                                                  gTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGc                                       >  2:8097470/1‑93 (MQ=255)
                                                  gTGGATTTACGCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGc                                       >  2:8000937/1‑93 (MQ=255)
                                                   tGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCa                                      <  2:1692664/93‑1 (MQ=255)
                                                   tGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCa                                      <  1:7589670/93‑1 (MQ=255)
                                                   tGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCa                                      <  1:2451367/93‑1 (MQ=255)
                                                   tGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCa                                      >  2:412423/1‑93 (MQ=255)
                                                    ggATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAg                                     >  1:3042961/1‑93 (MQ=255)
                                                     gATTTACGCCATCACGTCCGGGCCATTTACATTGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAgg                                    >  2:4949928/1‑93 (MQ=255)
                                                     gATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAgg                                    >  1:3289095/1‑93 (MQ=255)
                                                      aTTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGc                                   <  2:8701722/93‑1 (MQ=255)
                                                       tttACGCCATCACGTCTGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCATGct                                  >  2:2887243/1‑92 (MQ=255)
                                                       tttACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCa                                  >  2:6311961/1‑93 (MQ=255)
                                                        ttACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCAt                                 >  1:2348697/1‑93 (MQ=255)
                                                            gCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAACCGAGATGCCCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTaaa                             <  2:3352367/93‑1 (MQ=255)
                                                            gCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTaaa                             >  1:931951/1‑93 (MQ=255)
                                                            gCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTaaa                             >  1:5830054/1‑93 (MQ=255)
                                                               ctCACGTCCGGGCCATTTACATGGCGGGCACAGCGAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACa                          <  1:5361943/92‑1 (MQ=255)
                                                               aTCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACa                          >  2:8281088/1‑93 (MQ=255)
                                                               aTCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACa                          >  2:46652/1‑93 (MQ=255)
                                                                tCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAg                         <  2:2675605/93‑1 (MQ=255)
                                                                tCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAg                         <  1:3983116/93‑1 (MQ=255)
                                                                 cACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGa                        <  2:8565809/93‑1 (MQ=255)
                                                                  aCGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGaa                       <  2:30598/93‑1 (MQ=255)
                                                                   cgaccGGCCCATTCACATGGCCCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAAt                      <  2:6935478/90‑1 (MQ=255)
                                                                   cGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAAt                      <  2:6403076/93‑1 (MQ=255)
                                                                    gTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATc                     <  2:8724031/93‑1 (MQ=255)
                                                                     tCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCt                    >  1:1036291/1‑93 (MQ=255)
                                                                      ccGGGCCATTTACATGGGGCCCAAAGCTAAGGAACCGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTg                   <  2:5126197/93‑1 (MQ=255)
                                                                       cGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGTATTAAAACAGAATCTGt                  >  2:3264642/1‑93 (MQ=255)
                                                                         ggCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTcc                >  1:3483937/1‑93 (MQ=255)
                                                                          gCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCa               >  2:4274373/1‑93 (MQ=255)
                                                                           ccATTTACATGGCGCGCACAGGTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAt              <  2:2112292/93‑1 (MQ=255)
                                                                           ccATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAt              <  1:5431937/93‑1 (MQ=255)
                                                                            cATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAtt             <  1:5201269/93‑1 (MQ=255)
                                                                            cATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAtt             <  1:751700/93‑1 (MQ=255)
                                                                               ttACATGGCGCGCACAGCTAAGGAAACGAGGTGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTc          >  2:1637010/1‑93 (MQ=255)
                                                                                tACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGGCAGCCTCGATCTGCAGGCATTAACACAGAATCTGTCCATTGTcc         >  2:3388415/1‑93 (MQ=255)
                                                                                 aCATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCg        >  2:4848284/1‑93 (MQ=255)
                                                                                    tGGGGCGCACAGCTAAGGAAACGAGATGGCCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCa     <  2:6803757/93‑1 (MQ=255)
                                                                                     ggCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAg    <  2:1849579/93‑1 (MQ=255)
                                                                                     ggCGCACACAGAAAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAg    <  1:2654450/93‑1 (MQ=255)
                                                                                       cgcgCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGc  >  1:4674012/1‑93 (MQ=255)
                                                                                       cgcgCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGc  <  2:1080734/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGC  >  NC_000913/1237993‑1238172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCG  >  NC_000913/1237990‑1238176
                                                                                             |                                                                                             
TGGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCG                                                                                               <  SRR4302541.8175021/94‑1 (MQ=60)
 GGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGC                                                                                              >  SRR4302541.5709593/1‑94 (MQ=60)
   TCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCAC                                                                                            >  SRR4302541.6290779/1‑94 (MQ=60)
   TCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCAC                                                                                            >  SRR4302541.3488977/1‑94 (MQ=60)
    CGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACA                                                                                           <  SRR4302541.8225646/94‑1 (MQ=60)
    CGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACA                                                                                           >  SRR4302541.1780818/1‑94 (MQ=60)
     GCACGCCAGCGATCCCATATGCGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGGCATTTACATGGCGCGCACAG                                                                                          >  SRR4302541.8052025/1‑94 (MQ=60)
     GCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAG                                                                                          <  SRR4302541.4865845/94‑1 (MQ=60)
      CACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGC                                                                                         >  SRR4302541.8629690/1‑94 (MQ=60)
      CACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGC                                                                                         >  SRR4302541.831967/1‑94 (MQ=60)
      CACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGC                                                                                         >  SRR4302541.8313193/1‑94 (MQ=60)
       ACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCT                                                                                        <  SRR4302541.3827489/94‑1 (MQ=60)
       ACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCT                                                                                        >  SRR4302541.8556260/1‑94 (MQ=60)
          CCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAG                                                                                     >  SRR4302541.5555999/1‑94 (MQ=60)
            AGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGA                                                                                   <  SRR4302541.63961/94‑1 (MQ=60)
            AGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGA                                                                                   >  SRR4302541.1694782/1‑94 (MQ=60)
              CGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAA                                                                                 >  SRR4302541.6031784/1‑94 (MQ=60)
               GATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAAC                                                                                <  SRR4302541.973420/94‑1 (MQ=60)
               GATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAAC                                                                                <  SRR4302541.7008712/94‑1 (MQ=60)
               GATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAAC                                                                                >  SRR4302541.6944849/1‑94 (MQ=60)
                ATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACG                                                                               <  SRR4302541.5273696/94‑1 (MQ=60)
                ATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACG                                                                               <  SRR4302541.3003495/94‑1 (MQ=60)
                ATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACG                                                                               >  SRR4302541.2664340/1‑94 (MQ=60)
                  CCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAG                                                                             <  SRR4302541.982805/94‑1 (MQ=60)
                  CCCATATGAGGATCTAAGCGTAGCGCGCTACAACCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAG                                                                             <  SRR4302541.6364682/94‑1 (MQ=60)
                   CCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGA                                                                            <  SRR4302541.2557191/94‑1 (MQ=60)
                   CCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGA                                                                            >  SRR4302541.4166869/1‑94 (MQ=60)
                    CATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACTTCCTTGCCATTTACATGGCGCGCACAGCTAATGAACCGAGAT                                                                           >  SRR4302541.7909870/1‑94 (MQ=60)
                     ATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATG                                                                          >  SRR4302541.3995007/1‑94 (MQ=60)
                     ATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATG                                                                          >  SRR4302541.5028229/1‑94 (MQ=60)
                      TATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGA                                                                         >  SRR4302541.5175409/1‑94 (MQ=60)
                       ATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAC                                                                        >  SRR4302541.7455827/1‑94 (MQ=60)
                       ATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAC                                                                        >  SRR4302541.7795500/1‑94 (MQ=60)
                         GAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCC                                                                      >  SRR4302541.3326106/1‑94 (MQ=60)
                            GATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTC                                                                   <  SRR4302541.2723923/94‑1 (MQ=60)
                               CTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGA                                                                >  SRR4302541.255807/1‑94 (MQ=60)
                               CTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGA                                                                >  SRR4302541.3717800/1‑94 (MQ=60)
                               CTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGA                                                                >  SRR4302541.7865759/1‑94 (MQ=60)
                                TAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGAT                                                               >  SRR4302541.5438770/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGGGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              >  SRR4302541.581177/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              <  SRR4302541.4897217/94‑1 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              >  SRR4302541.8876458/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              >  SRR4302541.7156351/1‑94 (MQ=60)
                                 AAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATA                                                              <  SRR4302541.388033/94‑1 (MQ=60)
                                    CGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAG                                                           <  SRR4302541.5073372/94‑1 (MQ=60)
                                    CGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAG                                                           >  SRR4302541.3046338/1‑94 (MQ=60)
                                    CGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAG                                                           >  SRR4302541.1195773/1‑94 (MQ=60)
                                     GTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGG                                                          <  SRR4302541.2709462/94‑1 (MQ=60)
                                      TAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGC                                                         <  SRR4302541.387861/94‑1 (MQ=60)
                                       AGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCC                                                        <  SRR4302541.54364/94‑1 (MQ=60)
                                       AGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCC                                                        <  SRR4302541.2387724/94‑1 (MQ=60)
                                       AGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCA                                                        >  SRR4302541.1340925/1‑94 (MQ=60)
                                       AGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCC                                                        >  SRR4302541.7659080/1‑94 (MQ=60)
                                        GCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCA                                                       <  SRR4302541.8181730/94‑1 (MQ=60)
                                          GCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGC                                                     <  SRR4302541.4113912/94‑1 (MQ=60)
                                          GCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGC                                                     >  SRR4302541.7540995/1‑94 (MQ=60)
                                           CGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCC                                                    <  SRR4302541.3535425/94‑1 (MQ=60)
                                           CGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCC                                                    >  SRR4302541.3028132/1‑94 (MQ=60)
                                            GCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCT                                                   >  SRR4302541.711050/1‑94 (MQ=60)
                                             CTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTC                                                  <  SRR4302541.1244422/94‑1 (MQ=60)
                                             CTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTC                                                  >  SRR4302541.2350112/1‑94 (MQ=60)
                                             CTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTC                                                  <  SRR4302541.5649842/94‑1 (MQ=60)
                                              ctCAGCCGTCGATTTACGCCACCACGTCCGGGCCATTTACATGGCGCCCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCG                                                 <  SRR4302541.6680441/92‑1 (MQ=60)
                                              TACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCG                                                 <  SRR4302541.2889236/94‑1 (MQ=60)
                                               gcgGCCGTGGATCTACGCCATCAGGTCCGGGCCATGTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGA                                                <  SRR4302541.2921881/91‑1 (MQ=60)
                                               ACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGA                                                <  SRR4302541.4326912/94‑1 (MQ=60)
                                                CAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGAT                                               <  SRR4302541.1807974/94‑1 (MQ=60)
                                                 AGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATC                                              <  SRR4302541.1404975/94‑1 (MQ=60)
                                                 AGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATC                                              <  SRR4302541.7879165/94‑1 (MQ=60)
                                                  gcgggcGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCT                                             <  SRR4302541.4525866/88‑1 (MQ=60)
                                                   CCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTG                                            <  SRR4302541.234550/94‑1 (MQ=60)
                                                   CCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTG                                            >  SRR4302541.1703958/1‑94 (MQ=60)
                                                   CCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTG                                            <  SRR4302541.6191991/94‑1 (MQ=60)
                                                    CGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGC                                           <  SRR4302541.8345540/94‑1 (MQ=60)
                                                     GTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCA                                          >  SRR4302541.2747963/1‑94 (MQ=60)
                                                      TGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAG                                         >  SRR4302541.3073721/1‑94 (MQ=60)
                                                      TGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAG                                         <  SRR4302541.2475744/94‑1 (MQ=60)
                                                      TGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAG                                         <  SRR4302541.7670752/94‑1 (MQ=60)
                                                      TGGATTTACCCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAG                                         <  SRR4302541.133550/94‑1 (MQ=60)
                                                       GGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGG                                        >  SRR4302541.3296190/1‑94 (MQ=60)
                                                       GGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGG                                        >  SRR4302541.3322222/1‑94 (MQ=60)
                                                          TTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCAT                                     >  SRR4302541.2371537/1‑94 (MQ=60)
                                                              CGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAA                                 >  SRR4302541.940759/1‑94 (MQ=60)
                                                              CGCCATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAA                                 >  SRR4302541.5889341/1‑94 (MQ=60)
                                                                CCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAAC                               >  SRR4302541.184092/1‑94 (MQ=60)
                                                                CCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAAC                               <  SRR4302541.2690749/94‑1 (MQ=60)
                                                                 CATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACA                              >  SRR4302541.4454902/1‑94 (MQ=60)
                                                                 CATCACGTCCGGGACATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACA                              >  SRR4302541.350802/1‑94 (MQ=60)
                                                                  CTCACGTCCGGGCCATTTACATGGCGGGCACAGCGAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAG                             <  SRR4302541.5417011/94‑1 (MQ=60)
                                                                  ATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAG                             <  SRR4302541.8421658/94‑1 (MQ=60)
                                                                   TCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGA                            <  SRR4302541.4022592/94‑1 (MQ=60)
                                                                       GTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCT                        >  SRR4302541.1045891/1‑94 (MQ=60)
                                                                       GTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCT                        >  SRR4302541.3441429/1‑94 (MQ=60)
                                                                        TCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTG                       >  SRR4302541.4070278/1‑94 (MQ=60)
                                                                          CGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTC                     <  SRR4302541.8332102/94‑1 (MQ=60)
                                                                           GGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCC                    >  SRR4302541.3518810/1‑94 (MQ=60)
                                                                            GGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCA                   <  SRR4302541.3904463/94‑1 (MQ=60)
                                                                             GCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAT                  <  SRR4302541.5167872/94‑1 (MQ=60)
                                                                              CCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATT                 >  SRR4302541.5743969/1‑94 (MQ=60)
                                                                              CCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATT                 <  SRR4302541.5487666/94‑1 (MQ=60)
                                                                              CCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGACCTGCAGGCATTAAAACAGAATCTGTCCATT                 >  SRR4302541.7526203/1‑94 (MQ=60)
                                                                               CATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTG                <  SRR4302541.758797/94‑1 (MQ=60)
                                                                               CATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTG                <  SRR4302541.5254830/94‑1 (MQ=60)
                                                                                ATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGT               >  SRR4302541.6014558/1‑94 (MQ=60)
                                                                                 TTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTC              <  SRR4302541.2460020/94‑1 (MQ=60)
                                                                                  TTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCC             <  SRR4302541.8705083/94‑1 (MQ=60)
                                                                                  TTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCC             <  SRR4302541.4215905/94‑1 (MQ=60)
                                                                                   TACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCG            <  SRR4302541.8580376/94‑1 (MQ=60)
                                                                                   TACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCG            <  SRR4302541.2591120/94‑1 (MQ=60)
                                                                                       TGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAG        >  SRR4302541.4852795/1‑94 (MQ=60)
                                                                                       TGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAG        <  SRR4302541.1388859/94‑1 (MQ=60)
                                                                                        GGCGCACACAGAAAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGG       <  SRR4302541.2680858/94‑1 (MQ=60)
                                                                                         GCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGC      >  SRR4302541.4721611/1‑94 (MQ=60)
                                                                                           GCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCG    >  SRR4302541.6812920/1‑94 (MQ=60)
                                                                                            CGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGTTCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCca   >  SRR4302541.3082002/1‑92 (MQ=60)
                                                                                            CGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGC   >  SRR4302541.7812075/1‑94 (MQ=60)
                                                                                             GCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCG  <  SRR4302541.1486739/94‑1 (MQ=60)
                                                                                             GCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCG  >  SRR4302541.3692441/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGGTCGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCG  >  NC_000913/1237990‑1238176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑