Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,305,135 T→C T14A (ACG→GCG)  b1248 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,305,1350TC99.6% 815.7 / ‑5.1 233T14A (ACG→GCG) b1248hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (106/126);  minor base A (0/1);  total (106/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 241 total aligned reads displayed.

CTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGTTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTG  >  NC_000913/1305045‑1305224
                                                                                           |                                                                                         
ctTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGctt                                                                                         >  1:5683990/1‑93 (MQ=255)
ctTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGCCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGctt                                                                                         <  2:4532458/93‑1 (MQ=255)
  tCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCa                                                                                       >  1:6120914/1‑93 (MQ=255)
     aaCTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCt                                                                                    <  1:3605928/93‑1 (MQ=255)
       cTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtc                                                                                  <  2:1657675/93‑1 (MQ=255)
        tGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtca                                                                                 >  2:4963129/1‑93 (MQ=255)
         gAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcag                                                                                <  2:2405171/93‑1 (MQ=255)
         gAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcag                                                                                >  2:5822515/1‑93 (MQ=255)
          aaGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcagt                                                                               <  1:655579/93‑1 (MQ=255)
          aaGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcagt                                                                               <  2:671818/93‑1 (MQ=255)
           aGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcagtc                                                                              >  2:1184785/1‑93 (MQ=255)
            gATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcagtca                                                                             >  1:4742187/1‑93 (MQ=255)
             aTTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcagtcag                                                                            <  2:3880300/93‑1 (MQ=255)
             aTTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcagtcag                                                                            <  2:126059/93‑1 (MQ=255)
             aTTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTtcagtcag                                                                            <  2:2788093/93‑1 (MQ=255)
              ttGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAgg                                                                           <  2:4020630/93‑1 (MQ=255)
               tGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGc                                                                          <  1:1564420/93‑1 (MQ=255)
                 aaCAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGa                                                                        >  2:801781/1‑93 (MQ=255)
                 aaCAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGa                                                                        >  1:1921444/1‑93 (MQ=255)
                  aCAGCAAAACATCAAATGGATCGAAATTGTCGGCAGCAAGTTCGAG‑AAGAAACTCGTAGGCCTGTTCTCGCGATTCATCTTCAGTCAGGCGAt                                                                       <  1:6465520/93‑1 (MQ=255)
                   cagcagAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGAtt                                                                      >  1:97569/1‑93 (MQ=255)
                   cagcagAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGAtt                                                                      <  1:1642591/93‑1 (MQ=255)
                    agcagAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTg                                                                     >  2:1492930/1‑93 (MQ=255)
                    agcagAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTg                                                                     >  2:315387/1‑93 (MQ=255)
                     gcagAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGt                                                                    <  2:4591803/93‑1 (MQ=255)
                      cagAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGtt                                                                   <  1:2981877/93‑1 (MQ=255)
                        gAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGttt                                                                  >  2:4370951/1‑93 (MQ=255)
                        gAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTa                                                                 >  2:3307955/1‑93 (MQ=255)
                        gAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTa                                                                 <  1:1093470/93‑1 (MQ=255)
                        gAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTa                                                                 <  1:4466127/93‑1 (MQ=255)
                        gAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTa                                                                 <  1:1240828/93‑1 (MQ=255)
                          aCATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGa                                                               <  2:4158299/93‑1 (MQ=255)
                           cATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGat                                                              <  2:4798704/93‑1 (MQ=255)
                            aTCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGatc                                                             >  1:5848276/1‑93 (MQ=255)
                               aGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGatccat                                                          >  2:2491147/1‑93 (MQ=255)
                                  tGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGatccatatc                                                       >  1:4975447/1‑93 (MQ=255)
                                  tGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGatccatatc                                                       >  2:5204983/1‑93 (MQ=255)
                                    gATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGatccatatcca                                                     <  2:3095916/93‑1 (MQ=255)
                                     aTCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGatccatatccat                                                    >  1:5186020/1‑93 (MQ=255)
                                      tCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGAtccatatccata                                                   >  2:6079344/1‑93 (MQ=255)
                                       cGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAg                                                  <  2:566775/93‑1 (MQ=255)
                                         agaTTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGtt                                                >  2:5092690/1‑93 (MQ=255)
                                         agaTTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGtt                                                >  1:2856028/1‑93 (MQ=255)
                                         agaTTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGtt                                                >  1:2927343/1‑93 (MQ=255)
                                         agaTTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGtt                                                <  2:2351466/93‑1 (MQ=255)
                                          gaTTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTta                                               <  2:5942232/93‑1 (MQ=255)
                                           aTTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTtac                                              >  1:5073109/1‑93 (MQ=255)
                                            ttGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTtact                                             <  1:4331384/93‑1 (MQ=255)
                                             tGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTttcta                                            <  2:1399805/93‑1 (MQ=255)
                                             tGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTtacta                                            >  1:1737553/1‑93 (MQ=255)
                                              gTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTtactac                                           >  1:5715375/1‑93 (MQ=255)
                                               tCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTAcc                                          <  1:2397473/93‑1 (MQ=255)
                                                 ggCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTg                                        <  1:5271032/93‑1 (MQ=255)
                                                  gCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCGTAGTTACTACCTGt                                       <  2:3035494/93‑1 (MQ=255)
                                                  gCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGt                                       <  2:2838797/93‑1 (MQ=255)
                                                  gCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGt                                       >  1:4605659/1‑93 (MQ=255)
                                                    aGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGttt                                     >  2:3616973/1‑93 (MQ=255)
                                                    aGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGttt                                     <  1:6246775/93‑1 (MQ=255)
                                                    aGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGttt                                     <  2:1806564/93‑1 (MQ=255)
                                                    aGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGttt                                     <  2:2055131/93‑1 (MQ=255)
                                                    aGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGttt                                     <  2:2236143/93‑1 (MQ=255)
                                                       cAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAAc                                  <  1:1410997/93‑1 (MQ=255)
                                                        aGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAAcc                                 <  1:2036743/93‑1 (MQ=255)
                                                        aGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAAcc                                 >  1:148566/1‑93 (MQ=255)
                                                        aGTTAGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAAcc                                 <  2:5250319/93‑1 (MQ=255)
                                                         gTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACct                                >  1:3273362/1‑93 (MQ=255)
                                                         gTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACct                                >  1:790719/1‑93 (MQ=255)
                                                          ttCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACctc                               <  2:1886610/93‑1 (MQ=255)
                                                          ttCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACctc                               <  1:4165025/93‑1 (MQ=255)
                                                          ttCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACctc                               <  1:3553802/93‑1 (MQ=255)
                                                            cGAG‑AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTg                             >  2:5776169/1‑93 (MQ=255)
                                                             gag‑aAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGt                            >  1:5297076/1‑93 (MQ=255)
                                                               g‑aAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTTTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTg                          <  2:4066315/93‑1 (MQ=255)
                                                               g‑aAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTg                          >  1:3362550/1‑93 (MQ=255)
                                                                 aaaaaTATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTgg                         <  2:5275442/93‑1 (MQ=255)
                                                                  aaaaTATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGc                        >  1:3760915/1‑93 (MQ=255)
                                                                    aaTATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGa                      >  2:5322685/1‑93 (MQ=255)
                                                                       atCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGt                   <  1:6240507/93‑1 (MQ=255)
                                                                        tCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTCCCTGTTTAACCTCTGTTGGCGACGtt                  <  2:142576/93‑1 (MQ=255)
                                                                         cGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGttt                 <  1:5624841/93‑1 (MQ=255)
                                                                          gTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGtttt                <  1:738458/93‑1 (MQ=255)
                                                                          gTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGtttt                >  1:5439311/1‑93 (MQ=255)
                                                                          gTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGtttt                <  1:2522419/93‑1 (MQ=255)
                                                                          gTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGtttt                <  1:1025672/93‑1 (MQ=255)
                                                                             ggCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACa             >  1:5040138/1‑93 (MQ=255)
                                                                             ggCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACa             <  2:2609858/93‑1 (MQ=255)
                                                                               ccTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGc           <  2:833673/93‑1 (MQ=255)
                                                                                 tGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCaa         <  2:3974569/93‑1 (MQ=255)
                                                                                  gTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAAc        >  2:506818/1‑93 (MQ=255)
                                                                                  gTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAAc        >  1:4028857/1‑93 (MQ=255)
                                                                                    tCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACgg      <  1:6049592/93‑1 (MQ=255)
                                                                                     cAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGa     <  2:3333612/93‑1 (MQ=255)
                                                                                      aaGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGAc    <  2:5033525/93‑1 (MQ=255)
                                                                                      aaGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGAc    <  1:1814916/93‑1 (MQ=255)
                                                                                      aaGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGAc    <  1:2199323/93‑1 (MQ=255)
                                                                                      aaGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGAc    >  1:1381610/1‑93 (MQ=255)
                                                                                      aaGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGATTAACCTCTGTTGGCGACGTTGTACAGCAACGGAc    >  1:860587/1‑93 (MQ=255)
                                                                                       aGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACt   >  2:5408198/1‑93 (MQ=255)
                                                                                       aGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACt   <  2:3801485/93‑1 (MQ=255)
                                                                                        gcgcTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTg  <  2:6422831/93‑1 (MQ=255)
                                                                                           |                                                                                         
CTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAG‑AAAAATATCGTAGGCCTGTTCAAGCGTTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTG  >  NC_000913/1305045‑1305224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGTTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTGAAGA  >  NC_000913/1305042‑1305228
                                                                                             |                                                                                             
GCTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGC                                                                                               >  SRR4302534.2801018/1‑94 (MQ=60)
GCTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGC                                                                                               >  SRR4302534.1676455/1‑94 (MQ=60)
GCTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGC                                                                                               >  SRR4302534.3100817/1‑94 (MQ=60)
 CTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCT                                                                                              >  SRR4302534.5763060/1‑94 (MQ=60)
 CTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCT                                                                                              >  SRR4302534.5193772/1‑94 (MQ=60)
 CTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCT                                                                                              >  SRR4302534.2378559/1‑94 (MQ=60)
 CTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCT                                                                                              >  SRR4302534.771236/1‑94 (MQ=60)
  TCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTT                                                                                             >  SRR4302534.5837914/1‑94 (MQ=60)
  TCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTT                                                                                             <  SRR4302534.1919381/94‑1 (MQ=60)
    TTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCA                                                                                           >  SRR4302534.5895905/1‑94 (MQ=60)
    TTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCA                                                                                           >  SRR4302534.6287404/1‑94 (MQ=60)
      CAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATC                                                                                         >  SRR4302534.5461410/1‑94 (MQ=60)
      CAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATC                                                                                         >  SRR4302534.2371356/1‑94 (MQ=60)
        AACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTT                                                                                       >  SRR4302534.3449818/1‑94 (MQ=60)
        AACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTT                                                                                       >  SRR4302534.934354/1‑94 (MQ=60)
        AACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTT                                                                                       <  SRR4302534.3702895/94‑1 (MQ=60)
         ACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTC                                                                                      <  SRR4302534.4697628/94‑1 (MQ=60)
            GAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGAGCTTCATCTTCAGT                                                                                   >  SRR4302534.583554/1‑94 (MQ=60)
             AAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTC                                                                                  >  SRR4302534.1837001/1‑94 (MQ=60)
             AAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTC                                                                                  <  SRR4302534.671545/94‑1 (MQ=60)
              AGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCA                                                                                 >  SRR4302534.4870917/1‑94 (MQ=60)
              AGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCA                                                                                 >  SRR4302534.3801102/1‑94 (MQ=60)
               GATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATtttcactcgg                                                                                >  SRR4302534.3503490/1‑84 (MQ=60)
               GATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAG                                                                                <  SRR4302534.1550770/94‑1 (MQ=60)
                ATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGG                                                                               <  SRR4302534.1462768/94‑1 (MQ=60)
                 TTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGC                                                                              <  SRR4302534.1068757/94‑1 (MQ=60)
                  TGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCG                                                                             <  SRR4302534.1605363/94‑1 (MQ=60)
                   GAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGA                                                                            <  SRR4302534.6616698/94‑1 (MQ=60)
                   GAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGA                                                                            >  SRR4302534.1971674/1‑94 (MQ=60)
                   GAACAGCAGAACACCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGA                                                                            <  SRR4302534.5519139/94‑1 (MQ=60)
                     ACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATT                                                                          >  SRR4302534.100173/1‑94 (MQ=60)
                     ACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATT                                                                          >  SRR4302534.5088945/1‑94 (MQ=60)
                     ACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCAAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTGCAGTCAGGCGATT                                                                          <  SRR4302534.6342515/94‑1 (MQ=60)
                     ACAGCAAAACATCAAATGGATCGAAATTGTCGGCAGCAAGTTCGAGAAGAAACTCGTAGGCCTGTTCTCGCGATTCATCTTCAGTCAGGCGATT                                                                          <  SRR4302534.6639664/94‑1 (MQ=32)
                      CAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTG                                                                         <  SRR4302534.1685423/94‑1 (MQ=60)
                         CAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTT                                                                      <  SRR4302534.3059900/94‑1 (MQ=60)
                         CAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTT                                                                      <  SRR4302534.2517382/94‑1 (MQ=60)
                          AGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTA                                                                     >  SRR4302534.6039615/1‑94 (MQ=60)
                          AGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTA                                                                     <  SRR4302534.1982745/94‑1 (MQ=60)
                           GAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAG                                                                    <  SRR4302534.5830660/94‑1 (MQ=60)
                           GAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAG                                                                    <  SRR4302534.1121917/94‑1 (MQ=60)
                           GAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAG                                                                    <  SRR4302534.4586895/94‑1 (MQ=60)
                           GAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAG                                                                    <  SRR4302534.1273529/94‑1 (MQ=60)
                            AACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGA                                                                   >  SRR4302534.5181927/1‑94 (MQ=60)
                            AACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGA                                                                   <  SRR4302534.708761/94‑1 (MQ=60)
                              CATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATC                                                                 <  SRR4302534.3795362/94‑1 (MQ=60)
                              CATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATC                                                                 >  SRR4302534.6007060/1‑94 (MQ=60)
                               ATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCC                                                                <  SRR4302534.1513316/94‑1 (MQ=60)
                                TCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCA                                                               <  SRR4302534.817719/94‑1 (MQ=60)
                                 CAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCAT                                                              >  SRR4302534.6074172/1‑94 (MQ=60)
                                    CTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATC                                                           >  SRR4302534.5110133/1‑94 (MQ=60)
                                       GATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCAT                                                        >  SRR4302534.5325804/1‑94 (MQ=60)
                                         TCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAG                                                      <  SRR4302534.1685542/94‑1 (MQ=60)
                                         TCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAG                                                      <  SRR4302534.1436074/94‑1 (MQ=60)
                                          CGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGT                                                     >  SRR4302534.4868125/1‑94 (MQ=60)
                                          CGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGT                                                     >  SRR4302534.2718796/1‑94 (MQ=60)
                                          CGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGT                                                     <  SRR4302534.2975472/94‑1 (MQ=60)
                                          CGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGT                                                     >  SRR4302534.1768642/1‑94 (MQ=60)
                                          CGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTGCAGTCAGGCGATTGTTTAGATCCATATCCATAGT                                                     <  SRR4302534.3096466/94‑1 (MQ=60)
                                           GAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTT                                                    >  SRR4302534.3004206/1‑94 (MQ=60)
                                           GAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTT                                                    >  SRR4302534.2931047/1‑94 (MQ=60)
                                             GATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTAC                                                  >  SRR4302534.5210066/1‑94 (MQ=60)
                                              ATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACT                                                 >  SRR4302534.5812336/1‑94 (MQ=60)
                                               TTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTA                                                >  SRR4302534.1782668/1‑94 (MQ=60)
                                               TTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTA                                                <  SRR4302534.4448516/94‑1 (MQ=60)
                                                TGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTAC                                               >  SRR4302534.5870221/1‑94 (MQ=60)
                                                 GTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACC                                              <  SRR4302534.4429737/94‑1 (MQ=60)
                                                  TCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCT                                             <  SRR4302534.2461176/94‑1 (MQ=60)
                                                   CGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTG                                            <  SRR4302534.967685/94‑1 (MQ=60)
                                                    GGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGT                                           <  SRR4302534.5413410/94‑1 (MQ=60)
                                                    GGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGT                                           >  SRR4302534.4730638/1‑94 (MQ=60)
                                                       AGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTA                                        <  SRR4302534.1950304/94‑1 (MQ=60)
                                                       AGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTA                                        <  SRR4302534.6416205/94‑1 (MQ=60)
                                                        GCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAA                                       >  SRR4302534.2213034/1‑94 (MQ=60)
                                                          CAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACC                                     >  SRR4302534.152438/1‑94 (MQ=60)
                                                          CAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACC                                     <  SRR4302534.1448114/94‑1 (MQ=60)
                                                           AGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCT                                    <  SRR4302534.2090000/94‑1 (MQ=60)
                                                           AGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCT                                    >  SRR4302534.810523/1‑94 (MQ=60)
                                                           AGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCT                                    >  SRR4302534.1208618/1‑94 (MQ=60)
                                                           AGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCT                                    >  SRR4302534.3360590/1‑94 (MQ=60)
                                                             TTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCT                                  <  SRR4302534.4277525/94‑1 (MQ=60)
                                                             TTCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCT                                  <  SRR4302534.3649377/94‑1 (MQ=60)
                                                              TCGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTG                                 <  SRR4302534.2420193/94‑1 (MQ=60)
                                                               CGAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGT                                >  SRR4302534.5440039/1‑94 (MQ=60)
                                                                GAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTT                               >  SRR4302534.1932999/1‑94 (MQ=60)
                                                                GAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTT                               <  SRR4302534.3692152/94‑1 (MQ=60)
                                                                GAGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTT                               <  SRR4302534.5392708/94‑1 (MQ=60)
                                                                 AGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTG                              <  SRR4302534.409938/94‑1 (MQ=60)
                                                                 AGAAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTG                              >  SRR4302534.3452612/1‑94 (MQ=60)
                                                                   AAAAATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGC                            >  SRR4302534.3861895/1‑94 (MQ=60)
                                                                      AATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGAC                         >  SRR4302534.4315981/1‑94 (MQ=60)
                                                                      AATATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGAC                         >  SRR4302534.5761825/1‑94 (MQ=60)
                                                                         ATCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTT                      <  SRR4302534.6409835/94‑1 (MQ=60)
                                                                          TCGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACTTGTTTAACCTTTGTTGGGGAAGTTT                     >  SRR4302534.764241/1‑94 (MQ=60)
                                                                           CGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTT                    >  SRR4302534.5585804/1‑94 (MQ=60)
                                                                           CGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTT                    >  SRR4302534.5308981/1‑94 (MQ=60)
                                                                           CGTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTT                    <  SRR4302534.5777064/94‑1 (MQ=60)
                                                                           CGTAGACCTGTTCATGCGCTTCATCTTCAGTCAGGCAATTGTTTAGTTCCATATCCATAGTTACTTCCTGTTTCACCTCTGTTGGCGACGTTTT                    <  SRR4302534.6649358/94‑1 (MQ=60)
                                                                            GTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTA                   <  SRR4302534.2589371/94‑1 (MQ=60)
                                                                            GTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTA                   <  SRR4302534.756729/94‑1 (MQ=60)
                                                                            GTAGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTA                   <  SRR4302534.1052096/94‑1 (MQ=60)
                                                                              AGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACA                 >  SRR4302534.140985/1‑94 (MQ=60)
                                                                              AGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACA                 <  SRR4302534.5245616/94‑1 (MQ=60)
                                                                              AGGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACA                 >  SRR4302534.5176259/1‑94 (MQ=60)
                                                                               GGCCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAG                >  SRR4302534.6311310/1‑94 (MQ=60)
                                                                                 CCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCA              <  SRR4302534.1607829/94‑1 (MQ=60)
                                                                                 CCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCA              >  SRR4302534.5164672/1‑94 (MQ=60)
                                                                                 CCTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCA              >  SRR4302534.623446/1‑94 (MQ=60)
                                                                                  CTGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAA             >  SRR4302534.2754135/1‑94 (MQ=60)
                                                                                   TGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAAC            >  SRR4302534.4136871/1‑94 (MQ=60)
                                                                                   TGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAAC            <  SRR4302534.515141/94‑1 (MQ=60)
                                                                                   TGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAAC            >  SRR4302534.4730581/1‑94 (MQ=60)
                                                                                   TGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAAC            >  SRR4302534.3557266/1‑94 (MQ=60)
                                                                                   TGTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAAC            <  SRR4302534.3042513/94‑1 (MQ=60)
                                                                                    GTTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACG           >  SRR4302534.4532329/1‑94 (MQ=60)
                                                                                     TTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGG          <  SRR4302534.6505124/94‑1 (MQ=60)
                                                                                     TTCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGG          >  SRR4302534.3348450/1‑94 (MQ=60)
                                                                                      TCAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGA         <  SRR4302534.6214069/94‑1 (MQ=60)
                                                                                       CAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGAC        <  SRR4302534.4227425/94‑1 (MQ=60)
                                                                                       CAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGAC        >  SRR4302534.5419871/1‑94 (MQ=60)
                                                                                       CAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGAC        >  SRR4302534.1417988/1‑94 (MQ=60)
                                                                                       CAAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGATTAACCTCTGTTGGCGACGTTGTACAGCAACGGAC        >  SRR4302534.882313/1‑94 (MQ=60)
                                                                                        AAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACT       <  SRR4302534.1862075/94‑1 (MQ=60)
                                                                                        AAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACT       >  SRR4302534.760336/1‑94 (MQ=60)
                                                                                        AAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACT       >  SRR4302534.1354523/1‑94 (MQ=60)
                                                                                        AAGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACT       <  SRR4302534.2257520/94‑1 (MQ=60)
                                                                                         AGCGCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTG      <  SRR4302534.6410310/94‑1 (MQ=60)
                                                                                            GCTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTGAAG   <  SRR4302534.1982786/94‑1 (MQ=60)
                                                                                             CTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTGAAGA  >  SRR4302534.748217/1‑94 (MQ=60)
                                                                                             CTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTGAAGA  >  SRR4302534.2589332/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCTCTTCAAACTGAAGATTGAACAGCAGAACATCAGCTGGATCGAGATTGTCGGCAGCCAGTTCGAGAAAAATATCGTAGGCCTGTTCAAGCGTTTCATCTTCAGTCAGGCGATTGTTTAGATCCATATCCATAGTTACTACCTGTTTAACCTCTGTTGGCGACGTTTTACAGCAACGGACTGAAGA  >  NC_000913/1305042‑1305228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑