Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,372,245 A→G S44S (TCA→TCG)  ycjP → putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,372,2450AG99.7% 1060.8 / ‑4.9 302S44S (TCA→TCG) ycjPputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (156/145);  minor base T (0/1);  total (156/146)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.91e-01

Only 100 of 310 total aligned reads displayed.

CTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTT  >  NC_000913/1372155‑1372334
                                                                                          |                                                                                         
cTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCt                                                                                         >  1:2377479/1‑93 (MQ=255)
cTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCt                                                                                         <  2:1300451/93‑1 (MQ=255)
    gggCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCAt                                                                                     >  1:2456061/1‑93 (MQ=255)
      gCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATcc                                                                                   <  2:3521439/93‑1 (MQ=255)
      gCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATcc                                                                                   >  2:5534022/1‑93 (MQ=255)
       cTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCt                                                                                  <  2:3992415/93‑1 (MQ=255)
         ggCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTAc                                                                                >  1:5468386/1‑93 (MQ=255)
         ggCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTAc                                                                                >  2:4722799/1‑93 (MQ=255)
          gcgcTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACg                                                                               >  1:5686370/1‑93 (MQ=255)
           cgcTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACgc                                                                              <  1:2578883/93‑1 (MQ=255)
           cgcTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCACGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACgc                                                                              <  2:3433610/93‑1 (MQ=255)
              tGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGTGAAAGAGGCGATCTCGCTGCATCCTACgctgc                                                                           <  1:5950661/93‑1 (MQ=255)
              tGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACgctgc                                                                           <  2:563449/93‑1 (MQ=255)
              tGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACgctgc                                                                           >  2:507875/1‑93 (MQ=255)
              tGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACgctgc                                                                           <  2:2970025/93‑1 (MQ=255)
              tGTTTCTCATCACCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACgctgc                                                                           >  1:1177805/1‑93 (MQ=255)
               gTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACgctgct                                                                          <  1:221585/93‑1 (MQ=255)
                    tcatcatcaCGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCgc                                                                     >  1:4956461/1‑93 (MQ=255)
                    tcagcatcaCGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGACGATCTCGCTCCATCCTACGCTGCTGCCgc                                                                     <  1:3500270/93‑1 (MQ=255)
                       tcatcaCGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCgcagc                                                                  <  2:4494784/93‑1 (MQ=255)
                           caCGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTg                                                              <  2:201175/93‑1 (MQ=255)
                           caCGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTg                                                              <  1:3918601/93‑1 (MQ=255)
                             cGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGa                                                            <  2:1662467/93‑1 (MQ=255)
                              gCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGAc                                                           <  2:403760/93‑1 (MQ=255)
                              gCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGAc                                                           <  1:6270251/93‑1 (MQ=255)
                                tGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGc                                                         <  1:804697/93‑1 (MQ=255)
                                tGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGc                                                         >  2:4879284/1‑93 (MQ=255)
                                tGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGc                                                         <  2:6154757/93‑1 (MQ=255)
                                 gTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCt                                                        >  2:1872349/1‑93 (MQ=255)
                                  tttCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTg                                                       >  1:2980281/1‑93 (MQ=255)
                                   ttCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTgg                                                      >  1:4745525/1‑93 (MQ=255)
                                   ttCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTgg                                                      >  2:3311351/1‑93 (MQ=255)
                                    tCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTggg                                                     >  2:1193002/1‑92 (MQ=255)
                                    tCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGa                                                     >  2:4289518/1‑93 (MQ=255)
                                      cATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGc                                                   <  2:6371507/93‑1 (MQ=255)
                                       aTTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCa                                                  <  2:2687704/93‑1 (MQ=255)
                                       aTTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCa                                                  >  2:2494001/1‑93 (MQ=255)
                                            ttGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACg                                             >  2:1424739/1‑93 (MQ=255)
                                            ttGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACg                                             >  1:6450361/1‑93 (MQ=255)
                                             tgtgATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGt                                            <  2:2229159/93‑1 (MQ=255)
                                              gtgATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTc                                           >  2:2906489/1‑93 (MQ=255)
                                              gtgATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTc                                           <  2:4775357/93‑1 (MQ=255)
                                              gtgATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTc                                           <  1:1576017/93‑1 (MQ=255)
                                                gATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGa                                         >  2:3782424/1‑93 (MQ=255)
                                                 aTGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGAc                                        <  1:3083876/93‑1 (MQ=255)
                                                  tGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACa                                       <  1:1386989/93‑1 (MQ=255)
                                                   gCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACAt                                      >  1:912773/1‑93 (MQ=255)
                                                     tgatgaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACAttt                                    <  1:6009581/93‑1 (MQ=255)
                                                     tgatgaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACAttt                                    <  2:5351414/93‑1 (MQ=255)
                                                     tgatgaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACAttt                                    >  2:1591587/1‑93 (MQ=255)
                                                     tgatgaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACAttt                                    >  1:3880099/1‑93 (MQ=255)
                                                      gatgaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACAtttt                                   <  1:181104/93‑1 (MQ=255)
                                                         gaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTaa                                >  1:274061/1‑93 (MQ=255)
                                                          aCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAAc                               >  1:2959012/1‑93 (MQ=255)
                                                          aCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAAc                               >  1:6122393/1‑93 (MQ=255)
                                                           ccTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAAcc                              <  2:3637528/93‑1 (MQ=255)
                                                              cGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCga                           <  1:4257637/93‑1 (MQ=255)
                                                              cGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCga                           <  2:2317527/93‑1 (MQ=255)
                                                                 tCAAGGGCGCGAAGGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCgatga                        <  2:2418221/93‑1 (MQ=255)
                                                                 tCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCgatga                        <  1:742590/93‑1 (MQ=255)
                                                                   aaGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAtt                      >  1:1114456/1‑93 (MQ=255)
                                                                   aaGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAtt                      >  2:786423/1‑93 (MQ=255)
                                                                   aaGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAtt                      >  1:1034615/1‑93 (MQ=255)
                                                                   aaGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAtt                      >  2:2096459/1‑93 (MQ=255)
                                                                    aGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAttt                     >  1:2674988/1‑93 (MQ=255)
                                                                    aGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAttt                     >  2:5454166/1‑93 (MQ=255)
                                                                     gggCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAtttt                    >  1:636080/1‑93 (MQ=255)
                                                                       gcgcGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTcc                  <  1:1967758/93‑1 (MQ=255)
                                                                         gcgAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCg                >  1:3570730/1‑93 (MQ=255)
                                                                         gcgAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCg                <  1:5194231/93‑1 (MQ=255)
                                                                          cgAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGt               <  1:670230/93‑1 (MQ=255)
                                                                           gAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGtt              <  1:1888296/93‑1 (MQ=255)
                                                                           gAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGtt              <  1:12641/93‑1 (MQ=255)
                                                                           gAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGtt              >  1:4629199/1‑93 (MQ=255)
                                                                           caaaGAGGCGCTCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGtt              <  1:3382004/92‑1 (MQ=255)
                                                                            aaaGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGttt             <  2:5860053/93‑1 (MQ=255)
                                                                            aaaGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGttt             <  1:4094304/93‑1 (MQ=255)
                                                                            aaaGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGttt             >  2:1190689/1‑93 (MQ=255)
                                                                             aaGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTg            <  2:3851069/93‑1 (MQ=255)
                                                                               gagGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTc          >  1:1145527/1‑93 (MQ=255)
                                                                               gagGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTc          >  2:4328340/1‑93 (MQ=255)
                                                                               gagGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTc          <  2:14890/93‑1 (MQ=255)
                                                                               gagGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTc          <  1:997417/93‑1 (MQ=255)
                                                                               gagGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTc          <  1:4493985/93‑1 (MQ=255)
                                                                               gagGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTc          <  1:3748090/93‑1 (MQ=255)
                                                                                 ggCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGa        >  1:581723/1‑93 (MQ=255)
                                                                                 ggCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGa        >  2:305700/1‑93 (MQ=255)
                                                                                 ggCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGa        >  2:4981199/1‑93 (MQ=255)
                                                                                  gCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGac       >  1:5321496/1‑93 (MQ=255)
                                                                                  gCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGac       <  2:4556605/93‑1 (MQ=255)
                                                                                   cGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGact      <  2:31802/93‑1 (MQ=255)
                                                                                     aTCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGactac    <  1:211751/93‑1 (MQ=255)
                                                                                     aTCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGactac    >  1:2541980/1‑93 (MQ=255)
                                                                                     aTCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGactac    >  2:6177223/1‑93 (MQ=255)
                                                                                     aTCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGactac    >  2:1102939/1‑93 (MQ=255)
                                                                                     aTCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGactac    >  2:688455/1‑93 (MQ=255)
                                                                                      tctcGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGactact   >  1:1507416/1‑93 (MQ=255)
                                                                                      tctcGCTCCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTACCCCGATGATTTTCCCGTTTGTCGactact   <  2:4002175/93‑1 (MQ=255)
                                                                                       ctcGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACtt  >  2:5127689/1‑93 (MQ=255)
                                                                                       ctcGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACtt  <  1:1660535/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTT  >  NC_000913/1372155‑1372334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCG  >  NC_000913/1372153‑1372337
                                                                                            |                                                                                            
TACTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGC                                                                                             >  SRR4302534.1221356/1‑94 (MQ=60)
 ACTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCT                                                                                            <  SRR4302534.4414305/94‑1 (MQ=60)
 ACTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCT                                                                                            >  SRR4302534.2440631/1‑94 (MQ=60)
   TGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGC                                                                                          <  SRR4302534.2445027/94‑1 (MQ=60)
   TGCGGGCTGGCGCTGTTTCTCATCATAACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGC                                                                                          >  SRR4302534.4010024/1‑94 (MQ=60)
    GCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCA                                                                                         <  SRR4302534.5591001/94‑1 (MQ=60)
    GCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCA                                                                                         <  SRR4302534.6551036/94‑1 (MQ=60)
     CGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCAT                                                                                        >  SRR4302534.284247/1‑94 (MQ=60)
     CGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCAT                                                                                        >  SRR4302534.5945367/1‑94 (MQ=60)
     CGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCAT                                                                                        >  SRR4302534.2521315/1‑94 (MQ=60)
       GGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCC                                                                                      >  SRR4302534.2528445/1‑94 (MQ=60)
        GCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCT                                                                                     <  SRR4302534.1249878/94‑1 (MQ=60)
         CTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTA                                                                                    <  SRR4302534.3547671/94‑1 (MQ=60)
          TGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTAC                                                                                   >  SRR4302534.5615784/1‑94 (MQ=60)
           GGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACG                                                                                  >  SRR4302534.2139818/1‑94 (MQ=60)
           GGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACG                                                                                  >  SRR4302534.5840362/1‑94 (MQ=60)
           GGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACG                                                                                  >  SRR4302534.676627/1‑94 (MQ=60)
            GCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGC                                                                                 >  SRR4302534.722016/1‑94 (MQ=60)
             CGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCT                                                                                <  SRR4302534.2647192/94‑1 (MQ=60)
             CGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCT                                                                                >  SRR4302534.121699/1‑94 (MQ=60)
              GCTGTTTCTCATCATGACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTG                                                                               <  SRR4302534.6064851/94‑1 (MQ=60)
              GCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTG                                                                               >  SRR4302534.1750715/1‑94 (MQ=60)
               CTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGC                                                                              >  SRR4302534.5484513/1‑94 (MQ=60)
               CTGTTTCTCATCACCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGC                                                                              >  SRR4302534.1208763/1‑94 (MQ=60)
                TGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGTGAAAGAGGCGATCTCGCTGCATCCTACGCTGCT                                                                             <  SRR4302534.6112237/94‑1 (MQ=60)
                 GTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTG                                                                            >  SRR4302534.2338303/1‑94 (MQ=60)
                 GTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTG                                                                            <  SRR4302534.2863681/94‑1 (MQ=60)
                 GTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTG                                                                            <  SRR4302534.227392/94‑1 (MQ=60)
                 GTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTG                                                                            >  SRR4302534.2686815/1‑94 (MQ=60)
                   TTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCTCTGCATCCTACGCTGCTGCC                                                                          <  SRR4302534.6492823/94‑1 (MQ=60)
                    TCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCG                                                                         >  SRR4302534.3144783/1‑94 (MQ=60)
                    TCGCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCG                                                                         <  SRR4302534.1300546/94‑1 (MQ=60)
                     CTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGC                                                                        >  SRR4302534.3739579/1‑94 (MQ=60)
                     CTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGC                                                                        >  SRR4302534.5090688/1‑94 (MQ=60)
                      TCAGCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGACGATCTCGCTCCATCCTACGCTGCTGCCGCA                                                                       <  SRR4302534.3594395/94‑1 (MQ=60)
                       CATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAG                                                                      >  SRR4302534.4250140/1‑94 (MQ=60)
                        ATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGC                                                                     <  SRR4302534.56743/94‑1 (MQ=60)
                        ATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGC                                                                     <  SRR4302534.2624884/94‑1 (MQ=60)
                         TCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCA                                                                    <  SRR4302534.1014576/94‑1 (MQ=60)
                          CATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAG                                                                   >  SRR4302534.3981037/1‑94 (MQ=60)
                          CATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAG                                                                   >  SRR4302534.5936444/1‑94 (MQ=60)
                           ATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGT                                                                  >  SRR4302534.6122281/1‑94 (MQ=60)
                            TCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTG                                                                 <  SRR4302534.1906506/94‑1 (MQ=60)
                             CACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGG                                                                >  SRR4302534.1960060/1‑94 (MQ=60)
                             CACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGG                                                                <  SRR4302534.4023625/94‑1 (MQ=60)
                             CACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGG                                                                <  SRR4302534.150169/94‑1 (MQ=60)
                              ACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGA                                                               >  SRR4302534.1160890/1‑94 (MQ=60)
                               CGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGAC                                                              >  SRR4302534.6232726/1‑94 (MQ=60)
                                GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACG                                                             <  SRR4302534.6440023/94‑1 (MQ=60)
                                GCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACG                                                             <  SRR4302534.6253403/94‑1 (MQ=60)
                                CCTGTTTCAATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACG                                                             <  SRR4302534.60406/94‑1 (MQ=60)
                                 CTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGC                                                            >  SRR4302534.2462359/1‑94 (MQ=60)
                                  TGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCT                                                           <  SRR4302534.824912/94‑1 (MQ=60)
                                  TGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGAT                                                           >  SRR4302534.1972801/1‑94 (MQ=60)
                                   GTTTCGATTTTTTGTGATGCTGATGGCCTGGTTCAAGGGCGCGGAAGAGGCGATCGCGCTGCAGCCTGCGCTGCTGCCGCAGCAGTGGACGCTG                                                          <  SRR4302534.3773923/94‑1 (MQ=60)
                                   GTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTG                                                          >  SRR4302534.3058268/1‑94 (MQ=60)
                                    TTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGG                                                         >  SRR4302534.596838/1‑94 (MQ=60)
                                    TTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGG                                                         >  SRR4302534.4874350/1‑94 (MQ=60)
                                    TTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGG                                                         <  SRR4302534.2875474/94‑1 (MQ=60)
                                      TCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAG                                                       >  SRR4302534.2596445/1‑94 (MQ=60)
                                      TCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAG                                                       <  SRR4302534.5628842/94‑1 (MQ=60)
                                      TCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAG                                                       >  SRR4302534.5431549/1‑94 (MQ=60)
                                       CCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGC                                                      <  SRR4302534.827949/94‑1 (MQ=60)
                                       CCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACACTGCTGCCGCAGCAGTGGACGCTGGAGC                                                      >  SRR4302534.4774097/1‑94 (MQ=60)
                                         ATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCAT                                                    <  SRR4302534.6587528/94‑1 (MQ=60)
                                         ATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCAT                                                    >  SRR4302534.950235/1‑94 (MQ=60)
                                           TTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTA                                                  <  SRR4302534.341276/94‑1 (MQ=60)
                                             TTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACG                                                >  SRR4302534.6624190/1‑94 (MQ=60)
                                             TTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACG                                                <  SRR4302534.4265316/94‑1 (MQ=60)
                                              TTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGT                                               <  SRR4302534.4000122/94‑1 (MQ=60)
                                               TGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTC                                              <  SRR4302534.2188012/94‑1 (MQ=60)
                                                GTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCG                                             <  SRR4302534.1617252/94‑1 (MQ=60)
                                                GTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCG                                             <  SRR4302534.1626761/94‑1 (MQ=60)
                                                GTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCG                                             <  SRR4302534.805826/94‑1 (MQ=60)
                                                GTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCG                                             >  SRR4302534.5397447/1‑94 (MQ=60)
                                                GTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCG                                             >  SRR4302534.4440220/1‑94 (MQ=60)
                                                 TGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGA                                            >  SRR4302534.5331893/1‑94 (MQ=60)
                                                 TGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGA                                            <  SRR4302534.3679511/94‑1 (MQ=60)
                                                 TGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGA                                            <  SRR4302534.1854323/94‑1 (MQ=60)
                                                 TGATGCTGAGGTCCTCGTTCAAGGGCGCGAAAGAGTCGACCTCGCTGCATCCTACTCTGCTGCCGCAGCAGTGGTCGCTGGAGGATTACGTCtt                                            >  SRR4302534.6647333/1‑92 (MQ=60)
                                                  GATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGAC                                           <  SRR4302534.1571195/94‑1 (MQ=60)
                                                  GATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGAC                                           >  SRR4302534.1143813/1‑94 (MQ=60)
                                                  GATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGAC                                           >  SRR4302534.2610235/1‑94 (MQ=60)
                                                  GATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGAC                                           <  SRR4302534.861696/94‑1 (MQ=60)
                                                   ATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACA                                          <  SRR4302534.3164900/94‑1 (MQ=60)
                                                    TGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACAT                                         >  SRR4302534.935986/1‑94 (MQ=60)
                                                    TGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACAT                                         <  SRR4302534.1423489/94‑1 (MQ=60)
                                                      CTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTT                                       >  SRR4302534.3984289/1‑94 (MQ=60)
                                                       TGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTT                                      <  SRR4302534.6172860/94‑1 (MQ=60)
                                                       TGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTT                                      <  SRR4302534.2315977/94‑1 (MQ=60)
                                                       TGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTT                                      >  SRR4302534.615079/1‑94 (MQ=60)
                                                       TGATGACCTCGTTCAAGGGAGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGGCATTTT                                      <  SRR4302534.1544743/94‑1 (MQ=60)
                                                        GATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTT                                     <  SRR4302534.185867/94‑1 (MQ=60)
                                                         ATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTA                                    <  SRR4302534.1197861/94‑1 (MQ=60)
                                                          TGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAA                                   >  SRR4302534.281148/1‑94 (MQ=60)
                                                          TGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGGCGACATTTTTAA                                   >  SRR4302534.3102974/1‑94 (MQ=60)
                                                           GACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTGAC                                  >  SRR4302534.3057686/1‑94 (MQ=60)
                                                           GACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAAC                                  >  SRR4302534.3036551/1‑94 (MQ=60)
                                                           GACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAAC                                  >  SRR4302534.6288925/1‑94 (MQ=60)
                                                           GACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAAC                                  >  SRR4302534.4569549/1‑94 (MQ=60)
                                                            ACCTCGTTCAAGGGCGCGAATGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACC                                 >  SRR4302534.6467881/1‑94 (MQ=60)
                                                              CTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCG                               <  SRR4302534.4938553/94‑1 (MQ=60)
                                                              CTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCG                               >  SRR4302534.1092744/1‑94 (MQ=60)
                                                              CTCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACACTTTTAACCCG                               >  SRR4302534.1324876/1‑94 (MQ=60)
                                                               TCGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGA                              >  SRR4302534.2052843/1‑94 (MQ=60)
                                                                CGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGAT                             <  SRR4302534.4372575/94‑1 (MQ=60)
                                                                CGTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTAACTCGACATTTTTAACCCGAT                             >  SRR4302534.1011040/1‑94 (MQ=60)
                                                                 GTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATG                            <  SRR4302534.3438024/94‑1 (MQ=60)
                                                                 GTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATG                            <  SRR4302534.6296107/94‑1 (MQ=60)
                                                                 GTTCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCAGTACGTCGACATTTTTCACCCGATG                            >  SRR4302534.3943478/1‑94 (MQ=60)
                                                                   TCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAT                          <  SRR4302534.760988/94‑1 (MQ=60)
                                                                   TCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGAT                          >  SRR4302534.2772941/1‑94 (MQ=60)
                                                                   TCAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCA‑TACGTCGACATTTTTAAaccgaatcat                         >  SRR4302534.5236127/1‑84 (MQ=60)
                                                                    CAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATT                         <  SRR4302534.4202436/94‑1 (MQ=60)
                                                                    CAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATT                         >  SRR4302534.1143550/1‑94 (MQ=60)
                                                                    CAAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATT                         >  SRR4302534.1061312/1‑94 (MQ=60)
                                                                     AAGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTT                        >  SRR4302534.2745715/1‑94 (MQ=60)
                                                                      AGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTT                       >  SRR4302534.1084466/1‑94 (MQ=60)
                                                                      AGGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTT                       >  SRR4302534.651515/1‑94 (MQ=60)
                                                                       GGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTC                      <  SRR4302534.4208922/94‑1 (MQ=60)
                                                                       GGGCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTC                      <  SRR4302534.3646556/94‑1 (MQ=60)
                                                                         GCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCC                    <  SRR4302534.2019268/94‑1 (MQ=60)
                                                                         GCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCC                    <  SRR4302534.1193755/94‑1 (MQ=60)
                                                                         GCGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCC                    <  SRR4302534.4619940/94‑1 (MQ=60)
                                                                          CGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCG                   >  SRR4302534.3666774/1‑94 (MQ=60)
                                                                          CGCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCG                   <  SRR4302534.1579760/94‑1 (MQ=60)
                                                                           GCGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGT                  <  SRR4302534.5334296/94‑1 (MQ=60)
                                                                            CGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTT                 >  SRR4302534.4754818/1‑94 (MQ=60)
                                                                            CGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTT                 >  SRR4302534.1891709/1‑94 (MQ=60)
                                                                            CGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTT                 <  SRR4302534.686561/94‑1 (MQ=60)
                                                                            CGAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGAAATTTTTAACCCGATGATTTTACCGTT                 >  SRR4302534.2755250/1‑94 (MQ=60)
                                                                             GAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTT                >  SRR4302534.2727435/1‑94 (MQ=60)
                                                                             GAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTT                <  SRR4302534.12969/94‑1 (MQ=60)
                                                                             GAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTT                <  SRR4302534.1937533/94‑1 (MQ=60)
                                                                             GAAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTT                <  SRR4302534.1911590/94‑1 (MQ=60)
                                                                             CAAAGAGGCGCTCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTT                <  SRR4302534.3472668/94‑1 (MQ=60)
                                                                              AAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTG               <  SRR4302534.4204335/94‑1 (MQ=60)
                                                                              AAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTG               <  SRR4302534.3850715/94‑1 (MQ=60)
                                                                              AAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTG               >  SRR4302534.5125366/1‑94 (MQ=60)
                                                                              AAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTG               <  SRR4302534.5054956/94‑1 (MQ=60)
                                                                              AAAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTG               >  SRR4302534.6300490/1‑94 (MQ=60)
                                                                               AAGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGT              >  SRR4302534.1668541/1‑94 (MQ=60)
                                                                                AGAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTC             >  SRR4302534.1175532/1‑94 (MQ=60)
                                                                                 GAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCG            <  SRR4302534.3625802/94‑1 (MQ=60)
                                                                                 GAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCG            <  SRR4302534.4836423/94‑1 (MQ=60)
                                                                                 GAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCG            <  SRR4302534.1022973/94‑1 (MQ=60)
                                                                                 GAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCG            <  SRR4302534.3848768/94‑1 (MQ=60)
                                                                                 GAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCG            <  SRR4302534.4615614/94‑1 (MQ=60)
                                                                                 GAGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCG            <  SRR4302534.2182867/94‑1 (MQ=60)
                                                                                  AGGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGA           >  SRR4302534.595963/1‑94 (MQ=60)
                                                                                   GGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGAC          >  SRR4302534.4727765/1‑94 (MQ=60)
                                                                                   GGCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGAC          >  SRR4302534.5465019/1‑94 (MQ=60)
                                                                                    GCGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACT         <  SRR4302534.750999/94‑1 (MQ=60)
                                                                                     CGATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTA        >  SRR4302534.3581935/1‑94 (MQ=60)
                                                                                      GATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTAC       >  SRR4302534.2609412/1‑94 (MQ=60)
                                                                                      GATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTAC       >  SRR4302534.4090614/1‑94 (MQ=60)
                                                                                      GATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTAC       >  SRR4302534.3048538/1‑94 (MQ=60)
                                                                                       ATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACT      >  SRR4302534.1452638/1‑94 (MQ=60)
                                                                                       ATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACT      >  SRR4302534.1547066/1‑94 (MQ=60)
                                                                                       ATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACT      <  SRR4302534.217302/94‑1 (MQ=60)
                                                                                       ATCTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACT      <  SRR4302534.770167/94‑1 (MQ=60)
                                                                                         CTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTC    <  SRR4302534.1703788/94‑1 (MQ=60)
                                                                                         CTCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTC    >  SRR4302534.431688/1‑94 (MQ=60)
                                                                                         CTCGCTGCATCCTACGCTGCTGCCGCAGCAGGGGACGCTGGAACATTACGCCCACATTTTTAACCCGATGATTTTCTCGCTTGTATACTACTTC    >  SRR4302534.2366832/1‑94 (MQ=60)
                                                                                          TCGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCC   <  SRR4302534.2365632/94‑1 (MQ=60)
                                                                                           CGCTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCG  >  SRR4302534.3983862/1‑94 (MQ=60)
                                                                                            |                                                                                            
TACTGCGGGCTGGCGCTGTTTCTCATCATCACGCTGTTTCCATTTTTTGTGATGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCG  >  NC_000913/1372153‑1372337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑