Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,482,256	C→T	A391V (GCG→GTG)	hrpA →	helicase, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,482,256	0	C	T	99.2%	884.0 / ‑5.7	241	A391V (GCG→GTG)	hrpA	helicase, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (109/128); total (112/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

AATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCC  >  NC_000913/1482166‑1482345

gaTACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGgtgt                                                                                         >  2:6157661/2‑93 (MQ=255)

aaTACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGgtgt                                                                                         >  2:2803819/1‑93 (MQ=255)

 aTACGTTATCGACCCGAGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTc                                                                                        <  1:5738301/93‑1 (MQ=255)

 aTACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTc                                                                                        <  2:125813/93‑1 (MQ=255)

 aTACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTc                                                                                        >  1:2243901/1‑93 (MQ=255)

  tACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCt                                                                                       >  1:3894953/1‑93 (MQ=255)

  tACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCt                                                                                       >  1:2948316/1‑93 (MQ=255)

    cGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACATCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGc                                                                                     >  1:1909072/1‑93 (MQ=255)

      ttATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCa                                                                                   <  2:795436/93‑1 (MQ=255)

       tATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCGGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCaa                                                                                  <  2:4646470/93‑1 (MQ=255)

        aTCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAAt                                                                                 >  1:1145831/1‑93 (MQ=255)

            aCCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGc                                                                             >  1:5010781/1‑93 (MQ=255)

            aCCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGc                                                                             <  2:5924836/93‑1 (MQ=255)

            aCCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGc                                                                             <  2:4774012/93‑1 (MQ=255)

               ccGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTa                                                                          >  1:1697203/1‑93 (MQ=255)

               ccGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTa                                                                          >  2:3144120/1‑93 (MQ=255)

                cGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTaa                                                                         >  2:3862358/1‑93 (MQ=255)

                 ggTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTaaa                                                                        >  2:226347/1‑93 (MQ=255)

                 ggTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTaaa                                                                        >  2:5757664/1‑93 (MQ=255)

                 ggTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTaaa                                                                        >  1:4441357/1‑93 (MQ=255)

                   tACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAgg                                                                      <  2:5155779/93‑1 (MQ=255)

                    aCAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGc                                                                     <  2:5824587/93‑1 (MQ=255)

                     cAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGcc                                                                    <  1:2537341/93‑1 (MQ=255)

                      aGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCg                                                                   >  2:1382301/1‑93 (MQ=255)

                       gcgcGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGc                                                                  <  1:4146120/93‑1 (MQ=255)

                       gcgcGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGc                                                                  >  2:2519670/1‑93 (MQ=255)

                       gcgcGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGc                                                                  >  2:5244471/1‑93 (MQ=255)

                         gcgTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCtg                                                                <  1:1398257/93‑1 (MQ=255)

                         gcgTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCtg                                                                <  1:313816/93‑1 (MQ=255)

                          cgTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCtgt                                                               <  1:603263/93‑1 (MQ=255)

                           gTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCtgtg                                                              >  1:40632/1‑93 (MQ=255)

                           gTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTATCTGCCAATCAGCGTAAAGGCCGCtgtg                                                              >  1:3190207/1‑93 (MQ=255)

                             aTCAGCCGCTACAGCTATCTGACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCTCAGGCGACTGCCAATCAGCGTAAAGGCAGCTGTggc                                                            >  1:5944099/1‑92 (MQ=255)

                               cAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCg                                                          >  2:1887972/1‑93 (MQ=255)

                                aGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgt                                                         >  2:4402358/1‑93 (MQ=255)

                                 gCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtg                                                        >  2:2880131/1‑93 (MQ=255)

                                 gCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtg                                                        >  1:2793630/1‑93 (MQ=255)

                                  ccGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgt                                                       <  1:5137700/93‑1 (MQ=255)

                                  ccGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgt                                                       <  1:3794478/93‑1 (MQ=255)

                                   cGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgtg                                                      <  2:1959114/93‑1 (MQ=255)

                                   cGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgtg                                                      <  1:5222147/93‑1 (MQ=255)

                                    gCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgtgt                                                     >  1:3952511/1‑93 (MQ=255)

                                     cTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTc                                                    <  1:1196500/93‑1 (MQ=255)

                                     cTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTc                                                    >  1:1004224/1‑93 (MQ=255)

                                      tACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTcc                                                   >  2:2044776/1‑93 (MQ=255)

                                      tACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTcc                                                   <  2:5017356/93‑1 (MQ=255)

                                         aGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGaa                                                <  1:1563781/93‑1 (MQ=255)

                                          gCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAg                                               <  1:6476096/93‑1 (MQ=255)

                                          gCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAg                                               <  2:4069512/93‑1 (MQ=255)

                                            tATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGGAggg                                             >  1:2904834/1‑93 (MQ=255)

                                              tCGCCCCAAAGTTCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGAt                                           <  1:4719152/93‑1 (MQ=255)

                                              tCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGAt                                           <  1:5193590/93‑1 (MQ=255)

                                              tCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGAt                                           <  1:2892395/93‑1 (MQ=255)

                                               cGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATc                                          <  1:3262502/93‑1 (MQ=255)

                                                gCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCt                                         <  1:4270079/93‑1 (MQ=255)

                                                gCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCt                                         >  1:2139352/1‑93 (MQ=255)

                                                gCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCt                                         >  1:3374548/1‑93 (MQ=255)

                                                  aCCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGAGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGt                                       >  1:364295/1‑93 (MQ=255)

                                                   ccAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTa                                      <  1:4386078/93‑1 (MQ=255)

                                                   ccAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTa                                      <  2:4196374/93‑1 (MQ=255)

                                                   ccAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTa                                      <  1:3054952/93‑1 (MQ=255)

                                                     aaaGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTAtt                                    >  2:6029516/1‑93 (MQ=255)

                                                        gTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGt                                 >  2:4670692/1‑93 (MQ=255)

                                                        gTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGt                                 >  1:2162780/1‑93 (MQ=255)

                                                        gTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGt                                 <  2:3282647/93‑1 (MQ=255)

                                                         tGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtc                                <  1:6186250/93‑1 (MQ=255)

                                                         tGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtc                                <  2:5539801/93‑1 (MQ=255)

                                                          gCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtct                               >  2:3194903/1‑93 (MQ=255)

                                                          gCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtct                               <  2:3672889/93‑1 (MQ=255)

                                                           cAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtctc                              <  2:64329/93‑1 (MQ=255)

                                                           cAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtctc                              <  2:3408470/93‑1 (MQ=255)

                                                            aGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTctct                             <  1:89658/93‑1 (MQ=255)

                                                            aGCGTTTACCGATTGAGCCGATCTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTctct                             <  2:5362515/93‑1 (MQ=255)

                                                                tttGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTc                         <  1:4460476/93‑1 (MQ=255)

                                                                tttGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTc                         >  1:2849601/1‑93 (MQ=255)

                                                                tttGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTc                         >  2:4096518/1‑93 (MQ=255)

                                                                   gacGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGa                      >  1:4056323/3‑93 (MQ=255)

                                                                   gCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGa                      >  1:5541350/1‑93 (MQ=255)

                                                                    ccGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGaa                     <  1:1485913/93‑1 (MQ=255)

                                                                      gATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGa                   >  1:1890592/1‑93 (MQ=255)

                                                                      gATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGa                   >  2:4006148/1‑93 (MQ=255)

                                                                      gATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGa                   <  2:1056715/93‑1 (MQ=255)

                                                                       aTTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAc                  >  1:6305930/1‑93 (MQ=255)

                                                                       aTTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAc                  >  1:4565331/1‑93 (MQ=255)

                                                                       aTTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAc                  <  2:2470468/93‑1 (MQ=255)

                                                                        ttGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACg                 >  2:355589/1‑93 (MQ=255)

                                                                         tGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGa                >  2:2776821/1‑93 (MQ=255)

                                                                           aGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGAtt              <  2:1627600/93‑1 (MQ=255)

                                                                            gCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGAttt             <  2:72020/93‑1 (MQ=255)

                                                                             ccGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTc            <  1:5899966/93‑1 (MQ=255)

                                                                              cGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTcc           >  1:6238339/1‑93 (MQ=255)

                                                                               gATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCct          <  1:792990/93‑1 (MQ=255)

                                                                                aTTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCctc         <  1:4666316/93‑1 (MQ=255)

                                                                                aTTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCctc         <  2:193144/93‑1 (MQ=255)

                                                                                 tttCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCctct        <  2:4468206/93‑1 (MQ=255)

                                                                                  ttCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCtctc       <  1:4521627/93‑1 (MQ=255)

                                                                                    cccAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTcgc     >  1:666352/1‑93 (MQ=255)

                                                                                    cccAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTcgc     <  1:5385461/93‑1 (MQ=255)

                                                                                      cAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCgcgc   >  1:309317/1‑93 (MQ=255)

                                                                                       aGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGcc  >  2:6351675/1‑93 (MQ=255)

AATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCC  >  NC_000913/1482166‑1482345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGA  >  NC_000913/1482165‑1482349

AAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGT                                                                                             >  SRR4302534.4180891/1‑94 (MQ=60)

AAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGT                                                                                             >  SRR4302534.5713304/1‑94 (MQ=60)

 AATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTC                                                                                            >  SRR4302534.2303369/1‑94 (MQ=60)

 AATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTC                                                                                            >  SRR4302534.247171/1‑94 (MQ=60)

  ATACGTTATCGACCCGAGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCT                                                                                           <  SRR4302534.5893816/94‑1 (MQ=60)

  ATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCT                                                                                           >  SRR4302534.3999490/1‑94 (MQ=60)

  ATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCT                                                                                           >  SRR4302534.3025659/1‑94 (MQ=60)

    ACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACATCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGC                                                                                         >  SRR4302534.1958890/1‑94 (MQ=60)

       TTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAA                                                                                      >  SRR4302534.1548669/1‑94 (MQ=60)

        TATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAAT                                                                                     >  SRR4302534.1175848/1‑94 (MQ=60)

           CGCCCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAG                                                                                  <  SRR4302534.4075041/94‑1 (MQ=60)

           CGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAG                                                                                  >  SRR4302534.5828355/1‑94 (MQ=60)

            GACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGC                                                                                 >  SRR4302534.5146219/1‑94 (MQ=60)

               CCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTA                                                                              >  SRR4302534.1741305/1‑94 (MQ=60)

               CCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTA                                                                              >  SRR4302534.5510525/1‑94 (MQ=60)

                CCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAA                                                                             >  SRR4302534.916716/1‑94 (MQ=60)

                 CGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAA                                                                            >  SRR4302534.4561335/1‑94 (MQ=60)

                  GGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAG                                                                           <  SRR4302534.3064854/94‑1 (MQ=60)

                     ACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCC                                                                        <  SRR4302534.6236224/94‑1 (MQ=60)

                     ACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCC                                                                        >  SRR4302534.4721881/1‑94 (MQ=60)

                      CAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCG                                                                       <  SRR4302534.1474567/94‑1 (MQ=60)

                      CAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCG                                                                       <  SRR4302534.2239283/94‑1 (MQ=60)

                      CAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCG                                                                       <  SRR4302534.2604666/94‑1 (MQ=60)

                      CAGCGCGTATCACCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCG                                                                       <  SRR4302534.4573215/94‑1 (MQ=60)

                       AGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGC                                                                      >  SRR4302534.6238435/1‑94 (MQ=60)

                        GCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCT                                                                     <  SRR4302534.4258027/94‑1 (MQ=60)

                         CGCGTATCAGCCGCTAGAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTG                                                                    >  SRR4302534.531415/1‑94 (MQ=60)

                         CGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCATCGTTTGCGGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTG                                                                    <  SRR4302534.3265542/94‑1 (MQ=60)

                         CGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTG                                                                    <  SRR4302534.2053353/94‑1 (MQ=60)

                         CGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTG                                                                    >  SRR4302534.3204292/1‑94 (MQ=60)

                          GCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGT                                                                   <  SRR4302534.4903647/94‑1 (MQ=60)

                          GCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGT                                                                   <  SRR4302534.1435046/94‑1 (MQ=60)

                          GCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGT                                                                   <  SRR4302534.321908/94‑1 (MQ=60)

                           CGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTG                                                                  <  SRR4302534.617960/94‑1 (MQ=60)

                           CGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTG                                                                  >  SRR4302534.41716/1‑94 (MQ=60)

                           CGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTG                                                                  >  SRR4302534.1098910/1‑94 (MQ=60)

                           CGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTG                                                                  <  SRR4302534.2938041/94‑1 (MQ=60)

                           CGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTATCTGCCAATCAGCGTAAAGGCCGCTGTG                                                                  >  SRR4302534.3274666/1‑94 (MQ=60)

                             TATCAGCCGCTACCGCTATCGCACCAACGCGCCGCGTTTGCCGATTGAGCCGATTGCCCATGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGC                                                                >  SRR4302534.4275825/1‑94 (MQ=60)

                             TATCAGCCGCTACAGCTATCTGACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCTCAGGCGACTGCCAATCAGCGTAAAGGCAGCTGTGGC                                                                >  SRR4302534.6105492/1‑94 (MQ=60)

                                 AGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTG                                                            >  SRR4302534.2867108/1‑94 (MQ=60)

                                  GCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGT                                                           >  SRR4302534.159740/1‑94 (MQ=60)

                                   CCGCTACAGCTATCGCGCCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTG                                                          <  SRR4302534.2979006/94‑1 (MQ=60)

                                   CCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTG                                                          <  SRR4302534.3896356/94‑1 (MQ=60)

                                   CCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTG                                                          <  SRR4302534.5276374/94‑1 (MQ=60)

                                   CCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTG                                                          <  SRR4302534.681231/94‑1 (MQ=60)

                                   CCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTG                                                          <  SRR4302534.5629069/94‑1 (MQ=60)

                                    CGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGT                                                         >  SRR4302534.4058317/1‑94 (MQ=60)

                                    CGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGT                                                         <  SRR4302534.5363109/94‑1 (MQ=60)

                                     GCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTC                                                        <  SRR4302534.5252585/94‑1 (MQ=60)

                                     GCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTC                                                        >  SRR4302534.1029986/1‑94 (MQ=60)

                                     GCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTC                                                        <  SRR4302534.6090670/94‑1 (MQ=60)

                                     GCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTAGTGTGTC                                                        >  SRR4302534.4374545/1‑94 (MQ=60)

                                      CTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCC                                                       <  SRR4302534.1227983/94‑1 (MQ=60)

                                      CTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCC                                                       <  SRR4302534.36089/94‑1 (MQ=60)

                                         CAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAA                                                    >  SRR4302534.421231/1‑94 (MQ=60)

                                         CAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAA                                                    >  SRR4302534.1687632/1‑94 (MQ=60)

                                          AGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAG                                                   <  SRR4302534.1604707/94‑1 (MQ=60)

                                          AGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAG                                                   >  SRR4302534.2917990/1‑94 (MQ=60)

                                           GCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGG                                                  <  SRR4302534.6650581/94‑1 (MQ=60)

                                            CTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGGAGGG                                                 >  SRR4302534.2981093/1‑94 (MQ=60)

                                            CTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGG                                                 <  SRR4302534.1413328/94‑1 (MQ=60)

                                            CTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGG                                                 <  SRR4302534.4454054/94‑1 (MQ=60)

                                             TATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGA                                                <  SRR4302534.3809270/94‑1 (MQ=60)

                                              ATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGAT                                               <  SRR4302534.4171990/94‑1 (MQ=60)

                                               TCGCCCCAAAGTTCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATC                                              <  SRR4302534.4847314/94‑1 (MQ=60)

                                               TCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATC                                              <  SRR4302534.610133/94‑1 (MQ=60)

                                               TCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATC                                              <  SRR4302534.5345283/94‑1 (MQ=60)

                                               TCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATC                                              <  SRR4302534.5333628/94‑1 (MQ=60)

                                               TCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATC                                              <  SRR4302534.2968299/94‑1 (MQ=60)

                                                CGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCT                                             >  SRR4302534.3464977/1‑94 (MQ=60)

                                                CGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCT                                             >  SRR4302534.1253581/1‑94 (MQ=60)

                                                CGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCT                                             <  SRR4302534.2787728/94‑1 (MQ=60)

                                                CGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCT                                             >  SRR4302534.2195759/1‑94 (MQ=60)

                                                CGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCT                                             <  SRR4302534.3349379/94‑1 (MQ=60)

                                                CGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGAGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCT                                             >  SRR4302534.341708/1‑94 (MQ=60)

                                                 GCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTG                                            <  SRR4302534.4385498/94‑1 (MQ=60)

                                                  CACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGT                                           >  SRR4302534.1955131/1‑94 (MQ=60)

                                                  CACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGAGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGT                                           >  SRR4302534.373588/1‑94 (MQ=60)

                                                   ACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTA                                          >  SRR4302534.3679148/1‑94 (MQ=60)

                                                    CCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTAT                                         <  SRR4302534.736997/94‑1 (MQ=60)

                                                    CCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTAT                                         <  SRR4302534.3135105/94‑1 (MQ=60)

                                                    CCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTAT                                         <  SRR4302534.4504332/94‑1 (MQ=60)

                                                    CCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTAT                                         <  SRR4302534.3924425/94‑1 (MQ=60)

                                                        AGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGT                                     >  SRR4302534.2219904/1‑94 (MQ=60)

                                                         GTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAACGGCCGCTGAGGTCGTGTGTCCGAAGGGATCTGTATTCGTC                                    <  SRR4302534.6645609/94‑1 (MQ=60)

                                                          TGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCT                                   <  SRR4302534.6354621/94‑1 (MQ=60)

                                                           GCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTC                                  >  SRR4302534.4122812/1‑94 (MQ=60)

                                                            CAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCGGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCT                                 <  SRR4302534.4843564/94‑1 (MQ=60)

                                                            CAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCT                                 <  SRR4302534.4590384/94‑1 (MQ=60)

                                                             AGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTA                                <  SRR4302534.92047/94‑1 (MQ=60)

                                                             AGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTA                                <  SRR4302534.2728464/94‑1 (MQ=60)

                                                              GCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTAT                               <  SRR4302534.6382206/94‑1 (MQ=60)

                                                               CGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATT                              <  SRR4302534.3283583/94‑1 (MQ=60)

                                                                GTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTC                             >  SRR4302534.2924455/1‑94 (MQ=60)

                                                                 TTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCC                            <  SRR4302534.4581077/94‑1 (MQ=60)

                                                                  TTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCG                           <  SRR4302534.39825/94‑1 (MQ=60)

                                                                   TGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGA                          >  SRR4302534.5690932/1‑94 (MQ=60)

                                                                   TGACGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGA                          >  SRR4302534.4165121/1‑94 (MQ=60)

                                                                    GCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAA                         >  SRR4302534.4553201/1‑94 (MQ=60)

                                                                     CCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAG                        <  SRR4302534.1525004/94‑1 (MQ=60)

                                                                      TGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGA                       >  SRR4302534.3040404/1‑94 (MQ=60)

                                                                      CGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGG                       >  SRR4302534.4461449/1‑94 (MQ=60)

                                                                      CGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGA                       >  SRR4302534.1939900/1‑94 (MQ=60)

                                                                       GATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAC                      >  SRR4302534.4697997/1‑94 (MQ=60)

                                                                       GATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAC                      >  SRR4302534.4689099/1‑94 (MQ=60)

                                                                       GATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAC                      >  SRR4302534.6476447/1‑94 (MQ=60)

                                                                       GATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAC                      <  SRR4302534.651785/94‑1 (MQ=60)

                                                                         TTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGA                    <  SRR4302534.3173549/94‑1 (MQ=60)

                                                                           GAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATT                  <  SRR4302534.2202716/94‑1 (MQ=60)

                                                                           GAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATT                  >  SRR4302534.3319565/1‑94 (MQ=60)

                                                                           GAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATT                  >  SRR4302534.1203872/1‑94 (MQ=60)

                                                                            AGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTT                 <  SRR4302534.216996/94‑1 (MQ=60)

                                                                            AGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTT                 >  SRR4302534.4581726/1‑94 (MQ=60)

                                                                            AGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTT                 >  SRR4302534.2940067/1‑94 (MQ=60)

                                                                              CCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCC               <  SRR4302534.3009021/94‑1 (MQ=60)

                                                                              CCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCC               <  SRR4302534.606385/94‑1 (MQ=60)

                                                                              CCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCC               <  SRR4302534.6060161/94‑1 (MQ=60)

                                                                              CCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCC               >  SRR4302534.6407638/1‑94 (MQ=60)

                                                                              CCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCC               <  SRR4302534.6432172/94‑1 (MQ=60)

                                                                                GATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTC             <  SRR4302534.812858/94‑1 (MQ=60)

                                                                                GATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTC             >  SRR4302534.712219/1‑94 (MQ=60)

                                                                                GATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTC             >  SRR4302534.1865977/1‑94 (MQ=60)

                                                                                 ATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCT            <  SRR4302534.3222495/94‑1 (MQ=60)

                                                                                 ATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCT            <  SRR4302534.4793006/94‑1 (MQ=60)

                                                                                 ATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCT            <  SRR4302534.1879709/94‑1 (MQ=60)

                                                                                  TTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTC           <  SRR4302534.4652561/94‑1 (MQ=60)

                                                                                  TTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTC           >  SRR4302534.2274310/1‑94 (MQ=60)

                                                                                   TTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTGGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAAGATTTCCTCTCG          <  SRR4302534.5318068/94‑1 (MQ=60)

                                                                                   TTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCG          <  SRR4302534.4644111/94‑1 (MQ=60)

                                                                                    TCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGC         >  SRR4302534.3174717/1‑94 (MQ=60)

                                                                                    TCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGC         >  SRR4302534.682572/1‑94 (MQ=60)

                                                                                    TCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGC         >  SRR4302534.4987381/1‑94 (MQ=60)

                                                                                     CCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCG        <  SRR4302534.5530518/94‑1 (MQ=60)

                                                                                     CCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCG        >  SRR4302534.5440556/1‑94 (MQ=60)

                                                                                     CCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTATATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCG        <  SRR4302534.4003951/94‑1 (MQ=60)

                                                                                      CCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC       <  SRR4302534.6015326/94‑1 (MQ=60)

                                                                                      CCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC       >  SRR4302534.317303/1‑94 (MQ=60)

                                                                                        AGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCC     >  SRR4302534.210635/1‑94 (MQ=60)

                                                                                         GGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCG    <  SRR4302534.1068396/94‑1 (MQ=60)

                                                                                         GGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCG    <  SRR4302534.821830/94‑1 (MQ=60)

                                                                                         GGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCG    >  SRR4302534.4766853/1‑94 (MQ=60)

                                                                                         GGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCG    >  SRR4302534.2686799/1‑94 (MQ=60)

                                                                                          GTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGG   <  SRR4302534.4968491/94‑1 (MQ=60)

                                                                                           TGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGA  >  SRR4302534.4991461/1‑94 (MQ=60)

AAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGA  >  NC_000913/1482165‑1482349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`