Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,492,778 A→G G153G (GGT→GGC)  b1422 ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,492,7780AG100.0% 1014.5 / NA 292G153G (GGT→GGC) b1422putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (122/170);  total (122/170)

Only 100 of 305 total aligned reads displayed.

TGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATG  >  NC_000913/1492688‑1492867
                                                                                          |                                                                                         
  tCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCaa                                                                                       <  2:3537523/93‑1 (MQ=255)
   cGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAc                                                                                      <  1:4696689/93‑1 (MQ=255)
    gCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCaaac                                                                                     >  2:4486672/1‑91 (MQ=255)
     cAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAccc                                                                                    <  2:2795345/93‑1 (MQ=255)
     cAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAccc                                                                                    <  1:5235730/93‑1 (MQ=255)
       acgtGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTg                                                                                  <  1:2433002/90‑1 (MQ=255)
       aCTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTg                                                                                  >  1:4481430/1‑93 (MQ=255)
        cTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTga                                                                                 <  2:7663118/93‑1 (MQ=255)
         ttGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTgac                                                                                >  2:5981031/1‑93 (MQ=255)
          tGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGAGCCAACCCTgacg                                                                               <  2:1148772/93‑1 (MQ=255)
            cAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTgacgac                                                                             <  1:746361/93‑1 (MQ=255)
            cAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTgacgac                                                                             >  1:1905914/1‑93 (MQ=255)
              aGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAcat                                                                           <  2:223784/93‑1 (MQ=255)
               ggTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAcatc                                                                          <  1:4052834/93‑1 (MQ=255)
                gtcgtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAcatca                                                                         <  2:7092229/93‑1 (MQ=255)
                gtcgtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAcatca                                                                         <  1:181395/93‑1 (MQ=255)
                 tcgtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAcatcat                                                                        <  2:3907378/93‑1 (MQ=255)
                  ggtcTCTTTTTGGTGCTTATTAAACTTGACTATTACCGAAGGTAATAACCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCatt                                                                       <  1:4639498/92‑1 (MQ=255)
                    tctcTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCattat                                                                     >  2:3018313/1‑93 (MQ=255)
                     ctctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCattatt                                                                    >  2:6957309/1‑93 (MQ=255)
                      tctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGGGCCAACCCTGACGACATCAttatta                                                                   <  2:346311/93‑1 (MQ=255)
                      tctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCAttatta                                                                   >  2:5641488/1‑93 (MQ=255)
                      tctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCAttatta                                                                   >  2:5272612/1‑93 (MQ=255)
                       ctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTaa                                                                  >  1:1544244/1‑93 (MQ=255)
                        tttttGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAg                                                                 >  1:6591935/1‑93 (MQ=255)
                           ttgttgCTGATGAAACTTACCTATAACCGAGGGTAATATCCACAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGAcc                                                              <  2:1249751/93‑1 (MQ=255)
                             gttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACctt                                                            <  2:3287585/93‑1 (MQ=255)
                              ttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttc                                                           <  1:2981688/93‑1 (MQ=255)
                              ttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttc                                                           <  2:1760386/93‑1 (MQ=255)
                              ttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttc                                                           <  2:2911877/93‑1 (MQ=255)
                              gtgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttc                                                           <  1:6124852/92‑1 (MQ=255)
                               tgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttct                                                          <  2:668522/93‑1 (MQ=255)
                                gCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttctt                                                         >  1:5760473/1‑93 (MQ=255)
                                 cTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCttt                                                        <  2:8019347/93‑1 (MQ=255)
                                 cTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCttt                                                        <  2:6057021/93‑1 (MQ=255)
                                 cTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCttt                                                        <  1:3206774/93‑1 (MQ=255)
                                  tgatgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTa                                                       >  1:6857370/1‑93 (MQ=255)
                                  tgatgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTa                                                       <  1:7802173/93‑1 (MQ=255)
                                   gatgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTAc                                                      <  2:5179322/93‑1 (MQ=255)
                                    atgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACACTGACGACATCATTATTAAGACCTTCTTTACg                                                     >  1:8493398/1‑93 (MQ=255)
                                     tgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGa                                                    <  1:3673303/93‑1 (MQ=255)
                                      gaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGAt                                                   <  2:6295198/93‑1 (MQ=255)
                                        aaCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGa                                                 >  1:5153499/1‑93 (MQ=255)
                                        aaCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGa                                                 <  1:5581265/93‑1 (MQ=255)
                                         aCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGaa                                                <  1:2119674/93‑1 (MQ=255)
                                         aCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGaa                                                <  1:5295302/93‑1 (MQ=255)
                                          cTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAg                                               <  1:8315249/93‑1 (MQ=255)
                                          cTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAg                                               >  2:1200221/1‑93 (MQ=255)
                                           tGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGc                                              >  2:6502933/1‑93 (MQ=255)
                                            gACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCg                                             >  2:8324227/1‑93 (MQ=255)
                                            gACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCg                                             >  1:3901637/1‑93 (MQ=255)
                                              ccTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGAc                                           <  2:8288307/93‑1 (MQ=255)
                                                tataACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCg                                         >  2:8180494/1‑93 (MQ=255)
                                                tataACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACATTCTTTACGATGAAGCGACCg                                         >  1:7024241/1‑93 (MQ=255)
                                                  taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTc                                       <  1:7229113/93‑1 (MQ=255)
                                                   aaCCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTcc                                      <  1:735617/93‑1 (MQ=255)
                                                    aCCGAAGGTAATATCCCCAGTGCCGCAGTAGTTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCg                                     <  2:1944925/93‑1 (MQ=255)
                                                     ccGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCgg                                    <  1:2871668/93‑1 (MQ=255)
                                                     ccGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCgg                                    >  1:2519743/1‑93 (MQ=255)
                                                       gAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCa                                  >  2:5661655/1‑93 (MQ=255)
                                                        aaGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTGACGATGAAGCGACCGTCCGGCAg                                 >  2:4750500/1‑93 (MQ=255)
                                                         aGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAgt                                <  2:1098194/93‑1 (MQ=255)
                                                         aGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAgt                                >  1:6785711/1‑93 (MQ=255)
                                                           gTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATTATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAgtgt                              >  2:7229797/1‑93 (MQ=255)
                                                             aaTATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTg                            >  2:1584194/1‑93 (MQ=255)
                                                               tatCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAt                          <  1:3752502/93‑1 (MQ=255)
                                                               tatCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAt                          <  1:6330856/93‑1 (MQ=255)
                                                               tatCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAt                          >  2:1682290/1‑93 (MQ=255)
                                                                  ccccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGc                       >  1:7536780/1‑93 (MQ=255)
                                                                   cccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCg                      >  2:4344906/1‑93 (MQ=255)
                                                                    ccAGTGCCGCAGTAGGTAGTGCGCCACCCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGt                     <  1:1048567/93‑1 (MQ=255)
                                                                    ccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCgc                     >  2:8717771/1‑92 (MQ=255)
                                                                    ccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGt                     >  2:4159751/1‑93 (MQ=255)
                                                                    ccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGt                     >  2:7387643/1‑93 (MQ=255)
                                                                     cAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTc                    <  2:7883801/93‑1 (MQ=255)
                                                                      aGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCa                   >  2:8367043/1‑93 (MQ=255)
                                                                       gTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCaa                  <  2:6250974/93‑1 (MQ=255)
                                                                       gTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCaa                  <  1:1289092/93‑1 (MQ=255)
                                                                        tGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAg                 <  2:3396752/93‑1 (MQ=255)
                                                                         gCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGa                <  1:2627328/93‑1 (MQ=255)
                                                                             cAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActc            >  2:5798560/1‑93 (MQ=255)
                                                                             cAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActc            <  2:8575675/93‑1 (MQ=255)
                                                                             cAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActc            >  1:5398492/1‑93 (MQ=255)
                                                                             cAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActc            <  1:5966221/93‑1 (MQ=255)
                                                                              aGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActct           <  1:2280124/93‑1 (MQ=255)
                                                                               gtaggtagTGCGCCAACCCTGACGACATCCTTATTAAGACCTTCTTTACGATGCAGCGACCGTCCGGCCGGGTTGATGGCGTCAAGCACTCTg          >  2:6803164/1‑93 (MQ=255)
                                                                               gtaggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTg          >  2:5497230/1‑93 (MQ=255)
                                                                               gtaggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTg          >  1:3048914/1‑93 (MQ=255)
                                                                                gaggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGa         <  1:7304550/92‑1 (MQ=255)
                                                                                 aggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAc        <  2:3088964/93‑1 (MQ=255)
                                                                                  ggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACt       <  2:8767945/93‑1 (MQ=255)
                                                                                  ggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACt       >  1:1690788/1‑93 (MQ=255)
                                                                                  ggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACt       <  1:7919188/93‑1 (MQ=255)
                                                                                   gtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTg      <  2:3613865/93‑1 (MQ=255)
                                                                                    tagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGc     >  1:4953775/1‑93 (MQ=255)
                                                                                    gagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGc     <  2:2018498/92‑1 (MQ=255)
                                                                                     agTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGGCAAGAACTCTGACTGCa    >  1:7847261/1‑93 (MQ=255)
                                                                                      gTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCAt   <  2:4235233/93‑1 (MQ=255)
                                                                                      atGCGCCAACCCTGACGACATCATTATTAAGACCTGCTGTACGATGAAGCGACCGTCCGGCAGTGTTGATGCCGCCAAGAACTCTGACTGCAt   <  2:5302785/92‑1 (MQ=255)
                                                                                       tGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATg  <  1:916030/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATG  >  NC_000913/1492688‑1492867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTT  >  NC_000913/1492685‑1492871
                                                                                             |                                                                                             
CATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCG                                                                                               >  SRR4302541.5724982/1‑94 (MQ=60)
CATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCG                                                                                               >  SRR4302541.4261670/1‑94 (MQ=60)
 ATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGC                                                                                              >  SRR4302541.7681555/1‑94 (MQ=60)
   TGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCA                                                                                            <  SRR4302541.1784980/94‑1 (MQ=60)
   TGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCA                                                                                            >  SRR4302541.2347798/1‑94 (MQ=60)
     TCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAC                                                                                          <  SRR4302541.1107814/94‑1 (MQ=60)
      CGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCTGCAGTAGGTAGTGCGCCAACC                                                                                         <  SRR4302541.5749414/94‑1 (MQ=60)
      CGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACC                                                                                         <  SRR4302541.4744557/94‑1 (MQ=60)
        CAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCT                                                                                       <  SRR4302541.5289671/94‑1 (MQ=60)
        CAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCT                                                                                       <  SRR4302541.7978246/94‑1 (MQ=60)
         AACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTG                                                                                      >  SRR4302541.4526595/1‑94 (MQ=60)
         AACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTG                                                                                      <  SRR4302541.5290481/94‑1 (MQ=60)
          ACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGA                                                                                     <  SRR4302541.302244/94‑1 (MQ=60)
          ACGTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGA                                                                                     <  SRR4302541.2457091/94‑1 (MQ=60)
           CTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGAC                                                                                    >  SRR4302541.5239652/1‑94 (MQ=60)
           CTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGCAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCgtgag                                                                                    >  SRR4302541.7549613/1‑89 (MQ=60)
            TTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACG                                                                                   <  SRR4302541.2719996/94‑1 (MQ=60)
              GCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAC                                                                                 >  SRR4302541.1924231/1‑94 (MQ=60)
               CAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACA                                                                                <  SRR4302541.753431/94‑1 (MQ=60)
               CAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCGGACGACA                                                                                >  SRR4302541.7536571/1‑94 (MQ=60)
                AAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACAT                                                                               <  SRR4302541.3461620/94‑1 (MQ=60)
                  GGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCA                                                                             >  SRR4302541.2987857/1‑94 (MQ=60)
                  GGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCA                                                                             >  SRR4302541.6710973/1‑94 (MQ=60)
                  GGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCA                                                                             <  SRR4302541.4092827/94‑1 (MQ=60)
                   GTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCAT                                                                            <  SRR4302541.182755/94‑1 (MQ=60)
                   GTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCAT                                                                            <  SRR4302541.7219227/94‑1 (MQ=60)
                   GTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCAT                                                                            >  SRR4302541.463433/1‑94 (MQ=60)
                   GTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCAT                                                                            <  SRR4302541.6151993/94‑1 (MQ=60)
                   GTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCAT                                                                            <  SRR4302541.984871/94‑1 (MQ=60)
                    TCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATT                                                                           <  SRR4302541.1855072/94‑1 (MQ=60)
                     ggtctctttttggtgcttattaaacttgactattACCGAAGGTAATAACCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTA                                                                          <  SRR4302541.4686720/60‑1 (MQ=60)
                     CGTCTCTTTTTGTTGCAGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTA                                                                          <  SRR4302541.7912721/94‑1 (MQ=60)
                       TCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATT                                                                        >  SRR4302541.3760042/1‑94 (MQ=60)
                        CTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGCCATCATTATTA                                                                       >  SRR4302541.6903346/1‑94 (MQ=60)
                         TCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAA                                                                      >  SRR4302541.1559750/1‑94 (MQ=60)
                          CTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAG                                                                     <  SRR4302541.3444928/94‑1 (MQ=60)
                          CTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAG                                                                     >  SRR4302541.6659431/1‑94 (MQ=60)
                          CTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAG                                                                     <  SRR4302541.7388015/94‑1 (MQ=60)
                            TTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGAC                                                                   >  SRR4302541.8427204/1‑94 (MQ=60)
                              TTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCT                                                                 <  SRR4302541.7742368/94‑1 (MQ=60)
                              TTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCT                                                                 <  SRR4302541.4778606/94‑1 (MQ=60)
                               TGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTT                                                                <  SRR4302541.4307638/94‑1 (MQ=60)
                               TGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTT                                                                <  SRR4302541.2477533/94‑1 (MQ=60)
                                GTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTC                                                               <  SRR4302541.3100540/94‑1 (MQ=60)
                                 TTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCT                                                              <  SRR4302541.1825605/94‑1 (MQ=60)
                                 TTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCT                                                              <  SRR4302541.3011762/94‑1 (MQ=60)
                                 TTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCT                                                              <  SRR4302541.7220589/94‑1 (MQ=60)
                                 TTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCT                                                              <  SRR4302541.1450690/94‑1 (MQ=60)
                                 GTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCT                                                              <  SRR4302541.6187403/94‑1 (MQ=60)
                                  TGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTT                                                             >  SRR4302541.5819096/1‑94 (MQ=60)
                                  TGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTT                                                             <  SRR4302541.5321676/94‑1 (MQ=60)
                                   GCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTT                                                            >  SRR4302541.5581928/1‑94 (MQ=60)
                                    CTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTA                                                           >  SRR4302541.6930647/1‑94 (MQ=60)
                                    CTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTA                                                           <  SRR4302541.6159252/94‑1 (MQ=60)
                                    CTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTA                                                           <  SRR4302541.3239169/94‑1 (MQ=60)
                                     TGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTAC                                                          <  SRR4302541.7886222/94‑1 (MQ=60)
                                     TGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTAC                                                          >  SRR4302541.3123238/1‑94 (MQ=60)
                                      GATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGCCGACATCATTATTAAGACCTTCTTTACG                                                         >  SRR4302541.5079579/1‑94 (MQ=60)
                                      GATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACG                                                         <  SRR4302541.5841560/94‑1 (MQ=60)
                                      GATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACG                                                         <  SRR4302541.1387427/94‑1 (MQ=60)
                                      GATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACG                                                         <  SRR4302541.6987480/94‑1 (MQ=60)
                                      GATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACACTGACGACATCATTATTAAGACCTTCTTTACG                                                         >  SRR4302541.8584914/1‑94 (MQ=60)
                                       ATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGA                                                        >  SRR4302541.1629805/1‑94 (MQ=60)
                                        TGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGAT                                                       >  SRR4302541.545550/1‑94 (MQ=60)
                                        TGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGAT                                                       <  SRR4302541.7361431/94‑1 (MQ=60)
                                        TGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGAT                                                       >  SRR4302541.4531618/1‑94 (MQ=60)
                                        TGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGAT                                                       <  SRR4302541.4610181/94‑1 (MQ=60)
                                        TGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGAT                                                       <  SRR4302541.3710540/94‑1 (MQ=60)
                                        TGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGAT                                                       >  SRR4302541.4687652/1‑94 (MQ=60)
                                         GAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATG                                                      <  SRR4302541.7051713/94‑1 (MQ=60)
                                         GAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATG                                                      >  SRR4302541.985594/1‑94 (MQ=60)
                                         GAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATG                                                      >  SRR4302541.2849287/1‑94 (MQ=60)
                                          AAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGA                                                     >  SRR4302541.5206517/1‑94 (MQ=60)
                                          AAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGA                                                     >  SRR4302541.2619309/1‑94 (MQ=60)
                                          AAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGA                                                     <  SRR4302541.248723/94‑1 (MQ=60)
                                           AACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAA                                                    <  SRR4302541.2096116/94‑1 (MQ=60)
                                           AACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAA                                                    <  SRR4302541.5638399/94‑1 (MQ=60)
                                           AACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAA                                                    >  SRR4302541.5355729/1‑94 (MQ=60)
                                           AACCGACCTAAAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAA                                                    <  SRR4302541.6339484/94‑1 (MQ=60)
                                            ACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAG                                                   <  SRR4302541.5349810/94‑1 (MQ=60)
                                            ACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAG                                                   <  SRR4302541.2140188/94‑1 (MQ=60)
                                            ACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAG                                                   <  SRR4302541.2197492/94‑1 (MQ=60)
                                             CTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGC                                                  <  SRR4302541.8405611/94‑1 (MQ=60)
                                             CTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGC                                                  <  SRR4302541.2170302/94‑1 (MQ=60)
                                             CTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGC                                                  <  SRR4302541.6363853/94‑1 (MQ=60)
                                             CTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGC                                                  <  SRR4302541.8534512/94‑1 (MQ=60)
                                             CTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGC                                                  >  SRR4302541.2337342/1‑94 (MQ=60)
                                             CTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGC                                                  <  SRR4302541.3788777/94‑1 (MQ=60)
                                              TGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCG                                                 >  SRR4302541.3940671/1‑94 (MQ=60)
                                              TGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCG                                                 <  SRR4302541.1857399/94‑1 (MQ=60)
                                               GACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGA                                                >  SRR4302541.3903973/1‑94 (MQ=60)
                                                ACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGAC                                               <  SRR4302541.1102260/94‑1 (MQ=60)
                                                 CCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACC                                              <  SRR4302541.7370991/94‑1 (MQ=60)
                                                  CTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCG                                             >  SRR4302541.5437879/1‑94 (MQ=60)
                                                  CTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACATTCTTTACGATGAAGCGACCG                                             >  SRR4302541.7098920/1‑94 (MQ=60)
                                                    ATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGttaaagcgccggcc                                           >  SRR4302541.6962803/1‑80 (MQ=60)
                                                     TAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCC                                          <  SRR4302541.7306236/94‑1 (MQ=60)
                                                     TAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCC                                          <  SRR4302541.437646/94‑1 (MQ=60)
                                                      AACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCG                                         <  SRR4302541.742618/94‑1 (MQ=60)
                                                      AACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCG                                         >  SRR4302541.6584710/1‑94 (MQ=60)
                                                       ACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGG                                        >  SRR4302541.2545106/1‑94 (MQ=60)
                                                        CCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGC                                       <  SRR4302541.2900496/94‑1 (MQ=60)
                                                           AAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGT                                    >  SRR4302541.6858276/1‑94 (MQ=60)
                                                            AGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTG                                   >  SRR4302541.8315637/1‑94 (MQ=60)
                                                            AGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTG                                   <  SRR4302541.6722776/94‑1 (MQ=60)
                                                              GTAATCGCCCCAGGGCCGCAGTAGGTAGGTCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGTCCGTCCGGCAGTGTT                                 <  SRR4302541.6874469/94‑1 (MQ=60)
                                                               TAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTG                                <  SRR4302541.5124462/94‑1 (MQ=60)
                                                                 ATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAT                              >  SRR4302541.7413661/1‑94 (MQ=60)
                                                                 ATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAT                              >  SRR4302541.8379746/1‑94 (MQ=60)
                                                                  TATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATG                             <  SRR4302541.6395582/94‑1 (MQ=60)
                                                                  TATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATG                             <  SRR4302541.3790243/94‑1 (MQ=60)
                                                                   ATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGG                            <  SRR4302541.201736/94‑1 (MQ=60)
                                                                    TCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGC                           >  SRR4302541.3024681/1‑94 (MQ=60)
                                                                    TCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGC                           >  SRR4302541.7617338/1‑94 (MQ=60)
                                                                    TCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGC                           >  SRR4302541.7617339/1‑94 (MQ=60)
                                                                    TCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGC                           >  SRR4302541.5890603/1‑94 (MQ=60)
                                                                    TCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGC                           <  SRR4302541.4805652/94‑1 (MQ=60)
                                                                     CCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCG                          >  SRR4302541.7459587/1‑94 (MQ=60)
                                                                      CCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGT                         <  SRR4302541.2615918/94‑1 (MQ=60)
                                                                       gtggcgcgaaAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTC                        <  SRR4302541.7018319/84‑1 (MQ=60)
                                                                       CCAGTGCCGCAGTAGGTAGTGCGCCACCCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTC                        <  SRR4302541.1058304/94‑1 (MQ=60)
                                                                       CCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTC                        >  SRR4302541.3799812/1‑94 (MQ=60)
                                                                          GTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAG                     <  SRR4302541.1301842/94‑1 (MQ=60)
                                                                           TGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGA                    <  SRR4302541.6636449/94‑1 (MQ=60)
                                                                           TGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGA                    <  SRR4302541.4736373/94‑1 (MQ=60)
                                                                            GCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAA                   <  SRR4302541.2653543/94‑1 (MQ=60)
                                                                             CCGCAGTAGATAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAAC                  <  SRR4302541.8718759/94‑1 (MQ=60)
                                                                              CGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACT                 >  SRR4302541.1630822/1‑94 (MQ=60)
                                                                               GCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTC                >  SRR4302541.5453888/1‑94 (MQ=60)
                                                                                CAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCT               <  SRR4302541.4063876/94‑1 (MQ=60)
                                                                                CAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCT               <  SRR4302541.6027041/94‑1 (MQ=60)
                                                                                CAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCT               <  SRR4302541.5706084/94‑1 (MQ=60)
                                                                                 AGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTG              <  SRR4302541.2302168/94‑1 (MQ=60)
                                                                                 AGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTG              >  SRR4302541.7582422/1‑94 (MQ=60)
                                                                                 AGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTG              >  SRR4302541.3079761/1‑94 (MQ=60)
                                                                                  GTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGA             <  SRR4302541.8731514/94‑1 (MQ=60)
                                                                                   TAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAC            <  SRR4302541.2904910/94‑1 (MQ=60)
                                                                                   GAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAC            <  SRR4302541.7382526/94‑1 (MQ=60)
                                                                                    AGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACT           >  SRR4302541.7926655/1‑94 (MQ=60)
                                                                                    AGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACT           >  SRR4302541.1707393/1‑94 (MQ=60)
                                                                                     GGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTG          <  SRR4302541.8006339/94‑1 (MQ=60)
                                                                                      GTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGC         >  SRR4302541.5004422/1‑94 (MQ=60)
                                                                                       TAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCA        <  SRR4302541.5264639/94‑1 (MQ=60)
                                                                                       TAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCA        <  SRR4302541.394150/94‑1 (MQ=60)
                                                                                       TAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGGCAAGAACTCTGACTGCA        >  SRR4302541.7933368/1‑94 (MQ=60)
                                                                                         GTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATG      <  SRR4302541.7956809/94‑1 (MQ=60)
                                                                                          TGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGC     <  SRR4302541.7598916/94‑1 (MQ=60)
                                                                                          TGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGC     <  SRR4302541.924597/94‑1 (MQ=60)
                                                                                             GCCAACCCTGACGACATCATTATTAAGACCTTCTTTGCGATGAAGCGACCGTCGGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTT  <  SRR4302541.5999715/94‑1 (MQ=60)
                                                                                             GCCAACCCTGACGACATCATTATTAAGACCTTCTTTCCGATGAAGCGACCGTCCGGCAGTGTGGAGGGCGTCAAGAACTCTGAacgcagccgta  >  SRR4302541.2052709/1‑83 (MQ=60)
                                                                                             GCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTT  >  SRR4302541.6550131/1‑94 (MQ=60)
                                                                                             GCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTT  <  SRR4302541.8384159/94‑1 (MQ=60)
                                                                                             GCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTT  <  SRR4302541.7792548/94‑1 (MQ=60)
                                                                                             GCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTA  >  SRR4302541.2052710/1‑94 (MQ=60)
                                                                                             |                                                                                             
CATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTT  >  NC_000913/1492685‑1492871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑