Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,492,778 A→G G153G (GGT→GGC)  b1422 ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,492,7780AG100.0% 807.5 / NA 230G153G (GGT→GGC) b1422putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (105/125);  total (105/125)

Only 100 of 236 total aligned reads displayed.

TGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCAT  >  NC_000913/1492688‑1492866
                                                                                          |                                                                                        
   cGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAc                                                                                     >  2:5288839/1‑93 (MQ=255)
   cGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAc                                                                                     >  1:214103/1‑93 (MQ=255)
   cGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATAGCCCCAGTGCCGCAGTAGGTAGTGCGCCAAc                                                                                     <  1:182010/93‑1 (MQ=255)
    gCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCTCCAGTGCCGCAGTAGGTAGTGCGCCAAcc                                                                                    >  2:2041076/1‑93 (MQ=255)
    gCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAcc                                                                                    <  2:5508177/93‑1 (MQ=255)
     cAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAccc                                                                                   >  2:6427072/1‑93 (MQ=255)
     cAACTTGCAAGGTCGTCTCTTTTTGTCGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAccc                                                                                   <  2:4819664/93‑1 (MQ=255)
      aaCTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCt                                                                                  <  1:2575262/93‑1 (MQ=255)
        cTTGCAAGGTCGTCTATTTTTGTTGCTGATGAAACTAACATAAAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTga                                                                                <  1:6465004/93‑1 (MQ=255)
         ttGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTgac                                                                               >  2:6363265/1‑93 (MQ=255)
         ttGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTgac                                                                               <  2:3259241/93‑1 (MQ=255)
           gCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTgacga                                                                             >  2:4079980/1‑93 (MQ=255)
           gCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTgacga                                                                             >  2:5914246/1‑93 (MQ=255)
              aGGTCGTCTCTTTTTGTTGCTGATGACACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGCAGTGCGCCAACCCTGACGAcat                                                                          >  1:736551/1‑93 (MQ=255)
              aGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAcat                                                                          >  1:2303736/1‑93 (MQ=255)
               ggTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAcatc                                                                         >  1:3316149/1‑93 (MQ=255)
                gtcgtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGAcatca                                                                        <  1:1669744/93‑1 (MQ=255)
                   gtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCatta                                                                     <  1:1439111/93‑1 (MQ=255)
                   gtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCatta                                                                     >  1:2855153/1‑93 (MQ=255)
                       ctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTaa                                                                 <  1:2677767/93‑1 (MQ=255)
                       ctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTaa                                                                 <  1:1304544/93‑1 (MQ=255)
                        tttttGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAg                                                                >  1:3453249/1‑93 (MQ=255)
                        tttttGTTGCTGATGAAACTGACCTATAACCGAAGGGAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAg                                                                >  1:1363508/1‑93 (MQ=255)
                           ttgttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGAcc                                                             <  1:3660216/93‑1 (MQ=255)
                            tgttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACct                                                            >  2:6031563/1‑93 (MQ=255)
                            tgttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCAGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACct                                                            >  1:3804248/1‑93 (MQ=255)
                              ttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttc                                                          <  1:5831849/93‑1 (MQ=255)
                              ttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttc                                                          >  2:3131685/1‑93 (MQ=255)
                              ttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttc                                                          <  1:1032967/93‑1 (MQ=255)
                               tgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttct                                                         <  1:6240731/93‑1 (MQ=255)
                                gCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttctt                                                        <  2:456360/93‑1 (MQ=255)
                                gCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACcttctt                                                        <  2:5629264/93‑1 (MQ=255)
                                 cTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCttt                                                       <  1:3005749/93‑1 (MQ=255)
                                  tgatgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTa                                                      <  2:5478568/93‑1 (MQ=255)
                                  tgatgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTa                                                      <  1:495848/93‑1 (MQ=255)
                                     tgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGa                                                   <  2:1449300/93‑1 (MQ=255)
                                     tgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGa                                                   >  2:794877/1‑93 (MQ=255)
                                     tgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGa                                                   <  2:5327093/93‑1 (MQ=255)
                                      gaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGAt                                                  >  2:5160042/1‑93 (MQ=255)
                                       aaaCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATg                                                 <  1:6084357/93‑1 (MQ=255)
                                        aaCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGa                                                >  2:5837948/1‑93 (MQ=255)
                                        aaCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGa                                                <  1:1093824/93‑1 (MQ=255)
                                        aaCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGa                                                <  2:2895804/93‑1 (MQ=255)
                                           tGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGc                                             <  2:2650871/93‑1 (MQ=255)
                                           tGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGc                                             <  2:521645/93‑1 (MQ=255)
                                             aCCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGa                                           >  2:4612128/1‑93 (MQ=255)
                                              ccTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACACTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGAc                                          >  2:2116549/1‑93 (MQ=255)
                                               cTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGAcc                                         >  2:6170876/1‑93 (MQ=255)
                                                 ataACCGAAGGTAATATCCCCAGTGCCGCAGTAGGAAGTGCGGCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGATCGt                                       >  2:1078560/1‑93 (MQ=255)
                                                  taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTc                                      <  2:536340/93‑1 (MQ=255)
                                                  taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTc                                      <  2:1145780/93‑1 (MQ=255)
                                                   aaCCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTcc                                     <  1:4077118/93‑1 (MQ=255)
                                                    aCCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCg                                    >  2:4410555/1‑93 (MQ=255)
                                                    aCCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCg                                    >  1:5735181/1‑93 (MQ=255)
                                                     ccGAAGGTAATATCCCCAGTTCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCgg                                   <  1:3163652/93‑1 (MQ=255)
                                                     ccGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCgg                                   >  2:5575214/1‑93 (MQ=255)
                                                      cGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGc                                  <  1:1524597/93‑1 (MQ=255)
                                                      cGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGc                                  <  1:2110180/93‑1 (MQ=255)
                                                       gAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCa                                 <  2:5132998/93‑1 (MQ=255)
                                                        aaGGTAATATCCCCAGTTCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAg                                <  1:6392336/93‑1 (MQ=255)
                                                        aaGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAg                                <  2:3195494/93‑1 (MQ=255)
                                                           gTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAgtgt                             <  1:1883670/93‑1 (MQ=255)
                                                           gTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAgtgt                             <  1:3848571/93‑1 (MQ=255)
                                                            tAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCATCCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGtt                            <  2:3195814/93‑1 (MQ=255)
                                                              atatCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGa                          >  2:1308438/1‑93 (MQ=255)
                                                               tatCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAt                         >  1:4540085/1‑93 (MQ=255)
                                                               tatCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAt                         >  2:2225482/1‑93 (MQ=255)
                                                                atCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATg                        >  1:5929866/1‑93 (MQ=255)
                                                                 tCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATgg                       <  2:3891871/93‑1 (MQ=255)
                                                                  ccccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGc                      <  1:1055673/93‑1 (MQ=255)
                                                                  ccccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGc                      <  2:341221/93‑1 (MQ=255)
                                                                  ccccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGc                      <  2:2518985/93‑1 (MQ=255)
                                                                  ccccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGc                      <  1:2475126/93‑1 (MQ=255)
                                                                   cccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCg                     >  2:2578593/1‑93 (MQ=255)
                                                                    ccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGt                    >  2:3495086/1‑93 (MQ=255)
                                                                    ccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGt                    <  1:2618217/93‑1 (MQ=255)
                                                                    ccAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGGTGATGGCGt                    >  2:2300593/1‑93 (MQ=255)
                                                                      aGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATTATTATTAAGACCTTCTTTACGATTAAGCGACCGTCCGGCAGTGTTGATGGCGTCa                  <  1:786262/93‑1 (MQ=255)
                                                                       gTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCaa                 >  2:3637551/1‑93 (MQ=255)
                                                                       gTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCaa                 >  1:2173216/1‑93 (MQ=255)
                                                                          ccGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGaa              <  1:4368920/93‑1 (MQ=255)
                                                                            gCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAAct            <  1:1590465/93‑1 (MQ=255)
                                                                            gCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAAct            >  2:6298400/1‑93 (MQ=255)
                                                                             cAGTAGGTAGCGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActc           <  1:1788189/93‑1 (MQ=255)
                                                                              aGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActct          <  1:5160992/93‑1 (MQ=255)
                                                                              aGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActct          <  1:4787639/93‑1 (MQ=255)
                                                                              aGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActct          <  2:1053632/93‑1 (MQ=255)
                                                                              aGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAActct          >  1:5347327/1‑93 (MQ=255)
                                                                               gtaggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTg         <  2:5758197/93‑1 (MQ=255)
                                                                               gtaggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTg         <  2:3288915/93‑1 (MQ=255)
                                                                                taggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGa        <  1:3418623/93‑1 (MQ=255)
                                                                                taggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGa        <  2:1325840/93‑1 (MQ=255)
                                                                                 aggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAc       >  1:3042954/1‑93 (MQ=255)
                                                                                 aggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAc       <  2:4668615/93‑1 (MQ=255)
                                                                                 aggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAc       >  2:4655636/1‑93 (MQ=255)
                                                                                 aggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAc       <  2:3743967/93‑1 (MQ=255)
                                                                                  ggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACt      >  1:4135043/1‑93 (MQ=255)
                                                                                  ggtagTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACt      <  1:5048378/93‑1 (MQ=255)
                                                                                     agTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCa   <  1:4235783/93‑1 (MQ=255)
                                                                                      gTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCAt  >  1:3214692/1‑93 (MQ=255)
                                                                                          |                                                                                        
TGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCAT  >  NC_000913/1492688‑1492866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGT  >  NC_000913/1492685‑1492870
                                                                                             |                                                                                            
CATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCG                                                                                              >  SRR4302534.854864/1‑94 (MQ=60)
CATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCAAGTGCGGCAGAAGGAAGTGCG                                                                                              >  SRR4302534.1701127/1‑94 (MQ=60)
  TTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCC                                                                                            <  SRR4302534.1516303/94‑1 (MQ=60)
     TCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAAC                                                                                         >  SRR4302534.219718/1‑94 (MQ=60)
      CGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACC                                                                                        >  SRR4302534.3891060/1‑94 (MQ=60)
      CGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATAGCCCCAGTGCCGCAGTAGGTAGTGCGCCAACC                                                                                        <  SRR4302534.186796/94‑1 (MQ=60)
        CAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCT                                                                                      >  SRR4302534.3686054/1‑94 (MQ=60)
        CAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCT                                                                                      >  SRR4302534.4415657/1‑94 (MQ=60)
        CAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCT                                                                                      <  SRR4302534.2212986/94‑1 (MQ=60)
        CAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCT                                                                                      <  SRR4302534.1132377/94‑1 (MQ=60)
        CAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCT                                                                                      <  SRR4302534.1070128/94‑1 (MQ=60)
        CAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCT                                                                                      <  SRR4302534.3276775/94‑1 (MQ=60)
         AACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTG                                                                                     <  SRR4302534.2643481/94‑1 (MQ=60)
         AACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTG                                                                                     <  SRR4302534.3704898/94‑1 (MQ=60)
         AACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTG                                                                                     <  SRR4302534.1364239/94‑1 (MQ=60)
          ACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGA                                                                                    <  SRR4302534.1860766/94‑1 (MQ=60)
          ACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGA                                                                                    >  SRR4302534.5439021/1‑94 (MQ=60)
           CTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGAC                                                                                   <  SRR4302534.802468/94‑1 (MQ=60)
           CTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGAC                                                                                   <  SRR4302534.5165836/94‑1 (MQ=60)
           CTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGCAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGAC                                                                                   >  SRR4302534.235279/1‑94 (MQ=60)
           CTTGCAAGGTCGTCTATTTTTGTTGCTGATGAAACTAACATAAAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGAC                                                                                   <  SRR4302534.6639135/94‑1 (MQ=60)
            TTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACG                                                                                  <  SRR4302534.2710036/94‑1 (MQ=60)
                AAGGTCGTCTCTTTTTGTTGCTGATGACACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGCAGTGCGCCAACCCTGACGACAT                                                                              >  SRR4302534.754768/1‑94 (MQ=60)
                AAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACAT                                                                              >  SRR4302534.1755291/1‑94 (MQ=60)
                AAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACAT                                                                              >  SRR4302534.2364883/1‑94 (MQ=60)
                AAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACAT                                                                              >  SRR4302534.1990146/1‑94 (MQ=60)
                 AGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATC                                                                             >  SRR4302534.1176591/1‑94 (MQ=60)
                 AGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATC                                                                             >  SRR4302534.3404769/1‑94 (MQ=60)
                  GGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCA                                                                            <  SRR4302534.144253/94‑1 (MQ=60)
                   GTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCAT                                                                           <  SRR4302534.1713207/94‑1 (MQ=60)
                     CGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTA                                                                         >  SRR4302534.2930144/1‑94 (MQ=60)
                     CGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTA                                                                         <  SRR4302534.3393117/94‑1 (MQ=60)
                     CGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTA                                                                         <  SRR4302534.2043291/94‑1 (MQ=60)
                     CGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTA                                                                         >  SRR4302534.1588904/1‑94 (MQ=60)
                      GTCTCTTTTTGTTGCTGATGCAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTCGGTAGTGCGCCAACCCTGACGACATCATTAT                                                                        >  SRR4302534.221229/1‑94 (MQ=60)
                      GTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTAT                                                                        <  SRR4302534.1476995/94‑1 (MQ=60)
                      GTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTAT                                                                        >  SRR4302534.604963/1‑94 (MQ=60)
                          CTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAG                                                                    >  SRR4302534.3546079/1‑94 (MQ=60)
                          CTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAG                                                                    <  SRR4302534.2748559/94‑1 (MQ=60)
                          CTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAG                                                                    <  SRR4302534.1338959/94‑1 (MQ=60)
                          CTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAG                                                                    >  SRR4302534.1645741/1‑94 (MQ=60)
                          CTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGGAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAG                                                                    >  SRR4302534.1399433/1‑94 (MQ=60)
                           TTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGA                                                                   <  SRR4302534.1878027/94‑1 (MQ=60)
                              TTTTTGCTGATGAAACTGACCTATAACCTAAGGTAATATCCACAGTGCCGCAGTAGGTAGTGCGCCAGCCCTGACGACATCATTATTAAGACCT                                                                <  SRR4302534.3162198/94‑1 (MQ=60)
                              TTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCT                                                                <  SRR4302534.3758589/94‑1 (MQ=60)
                              TTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCAGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCT                                                                >  SRR4302534.3906395/1‑94 (MQ=60)
                               TGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTT                                                               >  SRR4302534.1017297/1‑94 (MQ=60)
                                 TTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCT                                                             <  SRR4302534.5990147/94‑1 (MQ=60)
                                 TTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCT                                                             >  SRR4302534.5492094/1‑94 (MQ=60)
                                 TTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCT                                                             <  SRR4302534.1059618/94‑1 (MQ=60)
                                  TGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTT                                                            <  SRR4302534.6410062/94‑1 (MQ=60)
                                    CTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTA                                                          >  SRR4302534.750934/1‑94 (MQ=60)
                                    CTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTA                                                          <  SRR4302534.3084382/94‑1 (MQ=60)
                                    CTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTA                                                          >  SRR4302534.5006162/1‑94 (MQ=60)
                                     TGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTAC                                                         <  SRR4302534.3097211/94‑1 (MQ=60)
                                     TGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTAC                                                         <  SRR4302534.508222/94‑1 (MQ=60)
                                      GATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACG                                                        <  SRR4302534.3238674/94‑1 (MQ=60)
                                       ATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGA                                                       >  SRR4302534.5225999/1‑94 (MQ=60)
                                        TGAAACTGACCTATAACCGAAGGTAATATCCCAAGTGCCGCAGTAGGTAGTGCGCCACCCCTGACGACATCATTATTAAGACCTTCTTTACGAT                                                      <  SRR4302534.6268478/94‑1 (MQ=60)
                                         GAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATG                                                     >  SRR4302534.2936232/1‑94 (MQ=60)
                                         GAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATG                                                     <  SRR4302534.4699205/94‑1 (MQ=60)
                                          AAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGA                                                    <  SRR4302534.6249802/94‑1 (MQ=60)
                                           AACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAA                                                   <  SRR4302534.2600350/94‑1 (MQ=60)
                                           AACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAA                                                   <  SRR4302534.1122280/94‑1 (MQ=60)
                                           AACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAA                                                   <  SRR4302534.5912378/94‑1 (MQ=60)
                                           AACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAA                                                   <  SRR4302534.182360/94‑1 (MQ=60)
                                            ACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAG                                                  >  SRR4302534.6503298/1‑94 (MQ=60)
                                             CTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGC                                                 <  SRR4302534.3432693/94‑1 (MQ=60)
                                             CTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGC                                                 >  SRR4302534.6600684/1‑94 (MQ=60)
                                              TGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCG                                                >  SRR4302534.933419/1‑94 (MQ=60)
                                              TGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCG                                                >  SRR4302534.2775191/1‑94 (MQ=60)
                                                ACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGAC                                              >  SRR4302534.5196401/1‑94 (MQ=60)
                                                 CCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACC                                             <  SRR4302534.2192587/94‑1 (MQ=60)
                                                 CCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACC                                             <  SRR4302534.2758472/94‑1 (MQ=60)
                                                  CTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGAGCG                                            >  SRR4302534.2408307/1‑94 (MQ=60)
                                                      AACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCG                                        >  SRR4302534.5890591/1‑94 (MQ=60)
                                                      AACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCG                                        <  SRR4302534.4186535/94‑1 (MQ=60)
                                                      AACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCG                                        <  SRR4302534.4325941/94‑1 (MQ=60)
                                                        CCGAAGGTAATATCCCCAGTTCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGC                                      <  SRR4302534.3247037/94‑1 (MQ=60)
                                                         CGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCA                                     <  SRR4302534.2165649/94‑1 (MQ=60)
                                                         CGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCA                                     <  SRR4302534.1564627/94‑1 (MQ=60)
                                                         CGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCA                                     <  SRR4302534.726150/94‑1 (MQ=60)
                                                          GAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAG                                    >  SRR4302534.1551656/1‑94 (MQ=60)
                                                           AAGGTAATATCCCCAGTTCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGT                                   <  SRR4302534.6564986/94‑1 (MQ=60)
                                                           AAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGT                                   >  SRR4302534.4373416/1‑94 (MQ=60)
                                                            AGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTG                                  <  SRR4302534.4521671/94‑1 (MQ=60)
                                                              GTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTT                                <  SRR4302534.1932752/94‑1 (MQ=60)
                                                              GTAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTT                                <  SRR4302534.3951919/94‑1 (MQ=60)
                                                               TAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTG                               <  SRR4302534.4228093/94‑1 (MQ=60)
                                                               TAATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTG                               >  SRR4302534.4116622/1‑94 (MQ=60)
                                                                 ATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAT                             >  SRR4302534.4663154/1‑94 (MQ=60)
                                                                 ATATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGAT                             <  SRR4302534.3525212/94‑1 (MQ=60)
                                                                  TATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATG                            >  SRR4302534.6090896/1‑94 (MQ=60)
                                                                   ATCCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGG                           <  SRR4302534.3942795/94‑1 (MQ=60)
                                                                     CCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCG                         <  SRR4302534.2540891/94‑1 (MQ=60)
                                                                     CCCCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCG                         <  SRR4302534.1082999/94‑1 (MQ=60)
                                                                     CCCCAGTGCCGCAGCAGGTAGTGCGCCAACCCCGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGGCCAGCAGTGTTGATGGCG                         >  SRR4302534.3330036/1‑94 (MQ=60)
                                                                       CCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTC                       >  SRR4302534.2555762/1‑94 (MQ=60)
                                                                       CCAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTC                       <  SRR4302534.2687507/94‑1 (MQ=60)
                                                                        CAGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCA                      <  SRR4302534.2645546/94‑1 (MQ=60)
                                                                         AGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATTATTATTAAGACCTTCTTTACGATTAAGCGACCGTCCGGCAGTGTTGATGGCGTCAA                     <  SRR4302534.805941/94‑1 (MQ=60)
                                                                         AGTGCCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAA                     >  SRR4302534.2230624/1‑94 (MQ=60)
                                                                             CCGCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAAC                 <  SRR4302534.4486658/94‑1 (MQ=60)
                                                                               GCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTC               <  SRR4302534.1632066/94‑1 (MQ=60)
                                                                               GCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTC               >  SRR4302534.4127374/1‑94 (MQ=60)
                                                                               GCAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTC               >  SRR4302534.148802/1‑94 (MQ=60)
                                                                                CAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCT              <  SRR4302534.5904948/94‑1 (MQ=60)
                                                                                CAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCT              <  SRR4302534.5051145/94‑1 (MQ=60)
                                                                                CAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCT              >  SRR4302534.5491506/1‑94 (MQ=60)
                                                                                CAGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCT              <  SRR4302534.1088346/94‑1 (MQ=60)
                                                                                CAGTAGGTAGCGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCT              <  SRR4302534.1834629/94‑1 (MQ=60)
                                                                                 AGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTG             <  SRR4302534.5300136/94‑1 (MQ=60)
                                                                                 AGTAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTG             <  SRR4302534.4917649/94‑1 (MQ=60)
                                                                                   TAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAC           >  SRR4302534.3122767/1‑94 (MQ=60)
                                                                                   TAGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGAC           <  SRR4302534.3510478/94‑1 (MQ=60)
                                                                                    AGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACT          >  SRR4302534.4246592/1‑94 (MQ=60)
                                                                                    AGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACT          <  SRR4302534.6346085/94‑1 (MQ=60)
                                                                                    AGGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACT          >  SRR4302534.6167663/1‑94 (MQ=60)
                                                                                     GGTAGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTG         <  SRR4302534.5184688/94‑1 (MQ=60)
                                                                                        AGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCAT      <  SRR4302534.4349964/94‑1 (MQ=60)
                                                                                        AGTGCGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCAT      >  SRR4302534.3300010/1‑94 (MQ=60)
                                                                                            CGCCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGT  <  SRR4302534.6555888/94‑1 (MQ=60)
                                                                                             |                                                                                            
CATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGT  >  NC_000913/1492685‑1492870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑