Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,520,926 A→G M55T (ATG→ACG)  b1451 ← putative outer membrane receptor for iron transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,9260AG100.0% 838.7 / NA 241M55T (ATG→ACG) b1451putative outer membrane receptor for iron transport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (112/129);  total (112/129)

Only 100 of 244 total aligned reads displayed.

TCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTC  >  NC_000913/1520836‑1521014
                                                                                          |                                                                                        
tCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGtc                                                                                        <  1:4648289/93‑1 (MQ=255)
 cTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGtct                                                                                       >  2:3697469/1‑93 (MQ=255)
   tGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTctcc                                                                                     <  2:4439847/93‑1 (MQ=255)
     cgcATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTctcctc                                                                                   <  2:1269311/93‑1 (MQ=255)
     cgcATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTctcctc                                                                                   <  2:2565178/93‑1 (MQ=255)
        aTAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGcc                                                                                <  1:3126464/93‑1 (MQ=255)
           gTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATc                                                                             >  2:2629518/1‑93 (MQ=255)
           gTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATc                                                                             <  2:2448827/93‑1 (MQ=255)
               tGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATccacc                                                                         <  1:3942489/93‑1 (MQ=255)
                gCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATccacca                                                                        >  2:2128961/1‑93 (MQ=255)
                gCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATccacca                                                                        <  1:4570871/93‑1 (MQ=255)
                gCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATccacca                                                                        <  2:3351518/93‑1 (MQ=255)
                gCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATccacca                                                                        >  2:6444532/1‑93 (MQ=255)
                 ccGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCcaccac                                                                       <  2:865542/93‑1 (MQ=255)
                 ccGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCcaccac                                                                       <  2:2240207/93‑1 (MQ=255)
                 ccGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCcaccac                                                                       <  1:1740609/93‑1 (MQ=255)
                   ggTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGc                                                                     <  2:305932/93‑1 (MQ=255)
                    gTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCt                                                                    <  1:1642179/93‑1 (MQ=255)
                    gTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCt                                                                    >  1:3841303/1‑93 (MQ=255)
                     ttttGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCtt                                                                   <  2:2390619/93‑1 (MQ=255)
                       ttGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAc                                                                 <  2:4705694/93‑1 (MQ=255)
                       ttGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAc                                                                 <  1:1982519/93‑1 (MQ=255)
                       ttGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAc                                                                 >  2:701877/1‑93 (MQ=255)
                        tGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAct                                                                >  2:4878828/1‑93 (MQ=255)
                        tGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAct                                                                <  2:3589574/93‑1 (MQ=255)
                         gTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActg                                                               >  1:4986736/1‑93 (MQ=255)
                          tACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgc                                                              <  1:3993558/93‑1 (MQ=255)
                             cTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgc                                                           <  1:2662237/93‑1 (MQ=255)
                               gCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgctg                                                         <  2:901234/93‑1 (MQ=255)
                               gCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgctg                                                         >  2:5026206/1‑93 (MQ=255)
                               gCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgctg                                                         <  1:2834625/93‑1 (MQ=255)
                                cAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTgg                                                        <  2:1909948/93‑1 (MQ=255)
                                cAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTgg                                                        <  2:266497/93‑1 (MQ=255)
                                 aaaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTggg                                                       <  1:4289519/93‑1 (MQ=255)
                                 aaaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTggg                                                       <  1:865352/93‑1 (MQ=255)
                                  aaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCCTCCACCACGCTTACTGCTGCTgcgg                                                      >  1:6379846/1‑93 (MQ=255)
                                  aaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTgggg                                                      >  1:2491928/1‑93 (MQ=255)
                                  aaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTgggg                                                      >  2:4001723/1‑93 (MQ=255)
                                  aaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGAGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTgggg                                                      >  2:4341894/1‑93 (MQ=255)
                                   aCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGt                                                     >  2:3229169/1‑93 (MQ=255)
                                   aCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGt                                                     >  2:432434/1‑93 (MQ=255)
                                     cAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTAt                                                   >  1:4099/1‑93 (MQ=255)
                                      aGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATc                                                  >  2:3015660/1‑93 (MQ=255)
                                      aGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATc                                                  >  2:280051/1‑93 (MQ=255)
                                      aGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATc                                                  >  1:238797/1‑93 (MQ=255)
                                       ggCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATcc                                                 <  1:512758/93‑1 (MQ=255)
                                         cgcGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAg                                               <  1:5393324/91‑1 (MQ=255)
                                          gcGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGt                                              >  1:5017381/2‑93 (MQ=255)
                                            gCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTc                                            >  2:1412275/1‑93 (MQ=255)
                                             ccGGTCCGTGCTTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGTTATCCAGTTCt                                           >  1:3296463/1‑93 (MQ=255)
                                             ccGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCt                                           <  1:2975412/93‑1 (MQ=255)
                                              cGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTg                                          >  1:5178551/1‑93 (MQ=255)
                                               ggTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGa                                         <  1:3594993/93‑1 (MQ=255)
                                                  cAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAac                                      <  1:4836678/93‑1 (MQ=255)
                                                  cAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAac                                      <  1:5580273/93‑1 (MQ=255)
                                                    gTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAacca                                    >  1:4325160/1‑93 (MQ=255)
                                                       aTTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCt                                 >  2:6283421/1‑93 (MQ=255)
                                                        ttCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtg                                <  1:99646/93‑1 (MQ=255)
                                                        ttCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtg                                >  1:2810085/1‑93 (MQ=255)
                                                          cGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtgcg                              <  2:5552212/93‑1 (MQ=255)
                                                          cGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtgcg                              >  1:723725/1‑93 (MQ=255)
                                                            gACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtgcggt                            >  2:2284859/1‑93 (MQ=255)
                                                            gACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtgcggt                            <  1:4214324/93‑1 (MQ=255)
                                                             aCAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtgcggtg                           <  1:1191352/93‑1 (MQ=255)
                                                              cAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtgcggtgc                          >  1:4503792/1‑93 (MQ=255)
                                                              cAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCtgcggtgc                          >  2:5508532/1‑93 (MQ=255)
                                                                 gTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGc                       <  1:2604784/93‑1 (MQ=255)
                                                                  ttAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCa                      >  2:5682980/1‑93 (MQ=255)
                                                                   tAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCAc                     <  1:3343935/93‑1 (MQ=255)
                                                                   tAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCAc                     >  1:236818/1‑93 (MQ=255)
                                                                       gcgcGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGAc                 >  1:1316953/1‑93 (MQ=255)
                                                                       gcgcGGTGATGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGAc                 >  1:6301371/1‑93 (MQ=255)
                                                                         gcgGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACaa               <  2:4505607/93‑1 (MQ=255)
                                                                           ggTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATc             >  1:3632644/1‑93 (MQ=255)
                                                                           ggTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATc             >  2:1757996/1‑93 (MQ=255)
                                                                            gtgtTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCa            <  2:5148075/93‑1 (MQ=255)
                                                                            gtgtTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCa            <  1:5999189/93‑1 (MQ=255)
                                                                            gtgtTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCa            >  1:1048264/1‑93 (MQ=255)
                                                                            gtgtTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGATGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCa            <  2:5829070/93‑1 (MQ=255)
                                                                             tgtTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCAt           <  1:1494086/93‑1 (MQ=255)
                                                                             tgtTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCAt           <  1:3017011/93‑1 (MQ=255)
                                                                              gtTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATa          >  2:1293847/1‑93 (MQ=255)
                                                                              gtTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATa          >  1:565440/1‑93 (MQ=255)
                                                                               ttGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAg         >  1:5917632/1‑93 (MQ=255)
                                                                                 gCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTc       <  1:1932001/93‑1 (MQ=255)
                                                                                  ccAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCt      >  2:1517295/1‑93 (MQ=255)
                                                                                  ccAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCt      <  1:5621503/93‑1 (MQ=255)
                                                                                   cAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTg     <  2:3966047/93‑1 (MQ=255)
                                                                                   cAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTg     <  2:1652797/93‑1 (MQ=255)
                                                                                   cAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTg     >  1:2772486/1‑93 (MQ=255)
                                                                                    aGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGt    <  2:6040388/93‑1 (MQ=255)
                                                                                    aGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGt    >  2:72066/1‑93 (MQ=255)
                                                                                    aGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGt    <  2:4774974/93‑1 (MQ=255)
                                                                                     ggCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGtt   >  1:2136694/1‑93 (MQ=255)
                                                                                     ggCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGtt   >  2:3800768/1‑93 (MQ=255)
                                                                                     ggCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGtt   >  2:5792035/1‑93 (MQ=255)
                                                                                     ggCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGtt   >  2:597762/1‑93 (MQ=255)
                                                                                     ggCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGtt   <  1:5714976/93‑1 (MQ=255)
                                                                                      gcgcGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTtc  >  1:4742255/1‑93 (MQ=255)
                                                                                      gcgcGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTtc  <  1:1526927/93‑1 (MQ=255)
                                                                                          |                                                                                        
TCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTC  >  NC_000913/1520836‑1521014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTCATCAG  >  NC_000913/1520833‑1521019
                                                                                             |                                                                                             
AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCG                                                                                               >  SRR4302534.2763504/1‑94 (MQ=60)
AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCG                                                                                               <  SRR4302534.3658031/94‑1 (MQ=60)
AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCG                                                                                               <  SRR4302534.4357309/94‑1 (MQ=60)
AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCG                                                                                               >  SRR4302534.2937835/1‑94 (MQ=60)
 AATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGT                                                                                              >  SRR4302534.3208548/1‑94 (MQ=60)
   TCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCT                                                                                            <  SRR4302534.3078289/94‑1 (MQ=60)
   TCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCT                                                                                            <  SRR4302534.4774475/94‑1 (MQ=60)
   TCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGAGCGGTGTTGCCAGGCGCGTCT                                                                                            >  SRR4302534.2705861/1‑94 (MQ=60)
      TGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCT                                                                                         <  SRR4302534.4389483/94‑1 (MQ=60)
       GCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTC                                                                                        <  SRR4302534.4856182/94‑1 (MQ=60)
       GCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTC                                                                                        <  SRR4302534.4956255/94‑1 (MQ=60)
       GCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTC                                                                                        <  SRR4302534.2887977/94‑1 (MQ=60)
          CATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCC                                                                                     >  SRR4302534.2766129/1‑94 (MQ=60)
          CATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCC                                                                                     <  SRR4302534.2056656/94‑1 (MQ=60)
           ATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCA                                                                                    <  SRR4302534.3208666/94‑1 (MQ=60)
           ATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCA                                                                                    >  SRR4302534.2334963/1‑94 (MQ=60)
            TAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCAT                                                                                   >  SRR4302534.3297007/1‑94 (MQ=60)
            TAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCAT                                                                                   >  SRR4302534.5806423/1‑94 (MQ=60)
             AGTTCTGCTGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATC                                                                                  <  SRR4302534.16504/94‑1 (MQ=60)
             AGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATC                                                                                  >  SRR4302534.601092/1‑94 (MQ=60)
                  TGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCA                                                                             <  SRR4302534.4048065/94‑1 (MQ=60)
                   GCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCAC                                                                            >  SRR4302534.3525606/1‑94 (MQ=60)
                   GCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCAC                                                                            <  SRR4302534.4694798/94‑1 (MQ=60)
                   GCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCAC                                                                            <  SRR4302534.757555/94‑1 (MQ=60)
                   GCCGGTTTTGTACATGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCAC                                                                            >  SRR4302534.3927203/1‑94 (MQ=60)
                    CCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACG                                                                           <  SRR4302534.1785806/94‑1 (MQ=60)
                     CGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGC                                                                          >  SRR4302534.5020672/1‑94 (MQ=60)
                      GGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCT                                                                         >  SRR4302534.3944486/1‑94 (MQ=60)
                       GTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTT                                                                        >  SRR4302534.3426235/1‑94 (MQ=60)
                       GTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTT                                                                        <  SRR4302534.1684999/94‑1 (MQ=60)
                         TTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAC                                                                      <  SRR4302534.627511/94‑1 (MQ=60)
                          TTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACT                                                                     <  SRR4302534.2034426/94‑1 (MQ=60)
                           TGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTG                                                                    >  SRR4302534.5121678/1‑94 (MQ=60)
                           TGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTG                                                                    <  SRR4302534.6111473/94‑1 (MQ=60)
                            GTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGC                                                                   <  SRR4302534.988482/94‑1 (MQ=60)
                            GTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGC                                                                   >  SRR4302534.5520030/1‑94 (MQ=60)
                             TAGCTGCGAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCT                                                                  <  SRR4302534.4474307/94‑1 (MQ=60)
                             TACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCT                                                                  <  SRR4302534.2031203/94‑1 (MQ=60)
                             TACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCT                                                                  <  SRR4302534.4100462/94‑1 (MQ=60)
                             TACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCT                                                                  >  SRR4302534.4838834/1‑94 (MQ=60)
                               CCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACATGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGC                                                                <  SRR4302534.2963016/94‑1 (MQ=60)
                                CTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCT                                                               <  SRR4302534.2732617/94‑1 (MQ=60)
                                 TGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTG                                                              >  SRR4302534.4640221/1‑94 (MQ=60)
                                  GCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGG                                                             <  SRR4302534.2909096/94‑1 (MQ=60)
                                    AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCCTCCACCACGCTTACTGCTGCTGCGG                                                           >  SRR4302534.6552177/1‑94 (MQ=60)
                                    AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG                                                           <  SRR4302534.4405738/94‑1 (MQ=60)
                                    AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG                                                           <  SRR4302534.2527479/94‑1 (MQ=60)
                                    AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG                                                           >  SRR4302534.646152/1‑94 (MQ=60)
                                    AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG                                                           >  SRR4302534.2558129/1‑94 (MQ=60)
                                    AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG                                                           <  SRR4302534.887209/94‑1 (MQ=60)
                                    AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG                                                           <  SRR4302534.6581590/94‑1 (MQ=60)
                                     AACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGT                                                          <  SRR4302534.3231679/94‑1 (MQ=60)
                                     AACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGT                                                          <  SRR4302534.4189427/94‑1 (MQ=60)
                                     AACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGT                                                          <  SRR4302534.5127351/94‑1 (MQ=60)
                                       CCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTAT                                                        <  SRR4302534.4496721/94‑1 (MQ=60)
                                       CCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTAT                                                        >  SRR4302534.4213/1‑94 (MQ=60)
                                       CCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTAT                                                        <  SRR4302534.4042227/94‑1 (MQ=60)
                                        CAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATC                                                       >  SRR4302534.245048/1‑94 (MQ=60)
                                        CAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATC                                                       >  SRR4302534.189960/1‑94 (MQ=60)
                                        CAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATC                                                       <  SRR4302534.1506486/94‑1 (MQ=60)
                                         AGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCC                                                      >  SRR4302534.1116333/1‑94 (MQ=60)
                                         AGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCC                                                      <  SRR4302534.3738620/94‑1 (MQ=60)
                                          GGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCA                                                     <  SRR4302534.5207601/94‑1 (MQ=60)
                                          GGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCA                                                     <  SRR4302534.525522/94‑1 (MQ=60)
                                           GCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAG                                                    >  SRR4302534.3687159/1‑94 (MQ=60)
                                            CGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGT                                                   <  SRR4302534.1963015/94‑1 (MQ=60)
                                            CGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGT                                                   >  SRR4302534.5152951/1‑94 (MQ=60)
                                            CGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGT                                                   <  SRR4302534.5538546/94‑1 (MQ=60)
                                             GCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTT                                                  >  SRR4302534.4695805/1‑94 (MQ=60)
                                              CGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTC                                                 <  SRR4302534.3189105/94‑1 (MQ=60)
                                               GCCGGTCCGTGCTTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGTTATCCAGTTCT                                                >  SRR4302534.3384407/1‑94 (MQ=60)
                                                CCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTG                                               <  SRR4302534.3053294/94‑1 (MQ=60)
                                                CCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTG                                               >  SRR4302534.5318074/1‑94 (MQ=60)
                                                CCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTG                                               >  SRR4302534.2435005/1‑94 (MQ=60)
                                                CCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTG                                               >  SRR4302534.4314583/1‑94 (MQ=60)
                                                 CGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGA                                              >  SRR4302534.4195122/1‑94 (MQ=60)
                                                  GGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAA                                             <  SRR4302534.3691684/94‑1 (MQ=60)
                                                  GGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAA                                             >  SRR4302534.4373344/1‑94 (MQ=60)
                                                    TCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAAC                                           >  SRR4302534.5848003/1‑94 (MQ=60)
                                                    TCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAAC                                           >  SRR4302534.1718586/1‑94 (MQ=60)
                                                     CAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACC                                          <  SRR4302534.5731035/94‑1 (MQ=60)
                                                     CAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACC                                          <  SRR4302534.4967894/94‑1 (MQ=60)
                                                      TGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCA                                         >  SRR4302534.1243654/1‑94 (MQ=60)
                                                      AGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCA                                         >  SRR4302534.4442178/1‑94 (MQ=60)
                                                        TGATTCGGGCAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACC                                       >  SRR4302534.5073419/1‑94 (MQ=60)
                                                        TGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACC                                       >  SRR4302534.574513/1‑94 (MQ=60)
                                                        TGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACC                                       >  SRR4302534.3718519/1‑94 (MQ=60)
                                                         GATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTATTGCTGCTGGGGTATCCAGTTCTGAAACCACCT                                      <  SRR4302534.5288504/94‑1 (MQ=60)
                                                         GATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCT                                      <  SRR4302534.2265209/94‑1 (MQ=60)
                                                         GATTCGGACAAGTTAATGCGCGGTCTTGCGAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCT                                      <  SRR4302534.2236168/94‑1 (MQ=60)
                                                          ATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTG                                     >  SRR4302534.2883968/1‑94 (MQ=60)
                                                           TTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGC                                    <  SRR4302534.102305/94‑1 (MQ=60)
                                                            TCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCG                                   >  SRR4302534.741593/1‑94 (MQ=60)
                                                               GACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTG                                <  SRR4302534.4327992/94‑1 (MQ=60)
                                                                ACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGC                               >  SRR4302534.4625693/1‑94 (MQ=60)
                                                                ACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGC                               <  SRR4302534.1222685/94‑1 (MQ=60)
                                                                 CAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCG                              <  SRR4302534.6391578/94‑1 (MQ=60)
                                                                 CAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCG                              >  SRR4302534.5682459/1‑94 (MQ=60)
                                                                    GTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCA                           <  SRR4302534.2673708/94‑1 (MQ=60)
                                                                     TTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCAC                          >  SRR4302534.243020/1‑94 (MQ=60)
                                                                     TTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCAC                          <  SRR4302534.140745/94‑1 (MQ=60)
                                                                      TAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACT                         <  SRR4302534.2847072/94‑1 (MQ=60)
                                                                      TAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACT                         <  SRR4302534.3433437/94‑1 (MQ=60)
                                                                       AATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTG                        >  SRR4302534.686501/1‑94 (MQ=60)
                                                                        ATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGA                       <  SRR4302534.6034480/94‑1 (MQ=60)
                                                                         TGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGAC                      >  SRR4302534.1560983/1‑94 (MQ=60)
                                                                         TGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGAC                      >  SRR4302534.5187299/1‑94 (MQ=60)
                                                                         TGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGAC                      >  SRR4302534.1351680/1‑94 (MQ=60)
                                                                         TGCGCGGTGATGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGAC                      >  SRR4302534.6471781/1‑94 (MQ=60)
                                                                             CGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATC                  >  SRR4302534.3730291/1‑94 (MQ=60)
                                                                              GGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAAGCACCTGCGGTGCGGCACTGACAATCA                 >  SRR4302534.443533/1‑94 (MQ=60)
                                                                              GGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCA                 >  SRR4302534.1075385/1‑94 (MQ=60)
                                                                               GTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCAT                <  SRR4302534.6162168/94‑1 (MQ=60)
                                                                                TGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATA               <  SRR4302534.3095990/94‑1 (MQ=60)
                                                                                TGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATA               >  SRR4302534.579340/1‑94 (MQ=60)
                                                                                TGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATA               <  SRR4302534.1533394/94‑1 (MQ=60)
                                                                                 GTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAG              <  SRR4302534.5352566/94‑1 (MQ=60)
                                                                                 GTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAG              >  SRR4302534.6078313/1‑94 (MQ=60)
                                                                                    GCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCT           <  SRR4302534.577019/94‑1 (MQ=60)
                                                                                    GCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCT           <  SRR4302534.1982511/94‑1 (MQ=60)
                                                                                     CCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTG          >  SRR4302534.1680733/1‑94 (MQ=60)
                                                                                     CCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTG          >  SRR4302534.2845437/1‑94 (MQ=60)
                                                                                     CCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTG          <  SRR4302534.2458805/94‑1 (MQ=60)
                                                                                     CCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTG          <  SRR4302534.5773620/94‑1 (MQ=60)
                                                                                     CCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTG          >  SRR4302534.4058067/1‑94 (MQ=60)
                                                                                       AGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTT        <  SRR4302534.3087762/94‑1 (MQ=60)
                                                                                       AGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTT        >  SRR4302534.2875742/1‑94 (MQ=60)
                                                                                       AGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTT        >  SRR4302534.2193031/1‑94 (MQ=60)
                                                                                       AGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTT        <  SRR4302534.2717686/94‑1 (MQ=60)
                                                                                        GGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTC       <  SRR4302534.5869811/94‑1 (MQ=60)
                                                                                        GGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTC       >  SRR4302534.4870987/1‑94 (MQ=60)
                                                                                        GGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTC       <  SRR4302534.2620933/94‑1 (MQ=60)
                                                                                         GCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTCA      <  SRR4302534.1567006/94‑1 (MQ=60)
                                                                                          CGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTCAAACCACCTGCGGTGCGGCACTGACAATCATAGCCTGTTCAT     >  SRR4302534.1787487/1‑94 (MQ=60)
                                                                                            CGTCTCCTCTCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTCATCA   <  SRR4302534.2393559/94‑1 (MQ=60)
                                                                                             GTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTCATCAG  <  SRR4302534.6015999/94‑1 (MQ=60)
                                                                                             GTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTCATCAG  >  SRR4302534.6056084/1‑94 (MQ=60)
                                                                                             GTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTCATCAG  >  SRR4302534.4156801/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTTCTGAAACCACCTGCGGTGCGGCACTGACAATCATAGTCTGTTCATCAG  >  NC_000913/1520833‑1521019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑