Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,533,898	A→G	intergenic (‑16/+63)	yddE ← / ← narV	hypothetical protein/cryptic nitrate reductase 2 gamma subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,533,898	0	A	G	99.6%	900.2 / ‑5.0	256	intergenic (‑16/+63)	yddE/narV	hypothetical protein/cryptic nitrate reductase 2 gamma subunit
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (132/123); total (133/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 261 total aligned reads displayed.

GAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATA  >  NC_000913/1533808‑1533987

    acaacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGgag                                                                                     <  1:2857473/93‑1 (MQ=255)

    acaacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGgag                                                                                     >  2:828258/1‑93 (MQ=255)

      aacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGaa                                                                                   >  2:6107846/1‑93 (MQ=255)

      aacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGaa                                                                                   >  1:1468135/1‑93 (MQ=255)

       accccGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAc                                                                                  <  2:3269321/90‑1 (MQ=255)

       acacCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAc                                                                                  >  2:2317474/1‑93 (MQ=255)

          ccGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTAt                                                                               <  1:4718493/93‑1 (MQ=255)

          ccGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTAt                                                                               >  1:5905665/1‑93 (MQ=255)

           cGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATc                                                                              <  1:3661052/93‑1 (MQ=255)

             gCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCAt                                                                            <  1:337963/93‑1 (MQ=255)

             gCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGCTCTCCTGTGGCTGGAAAACCATcct                                                                            >  1:1049153/1‑91 (MQ=255)

              cAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATa                                                                           >  2:5824790/1‑93 (MQ=255)

              cAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATa                                                                           >  2:5037668/1‑93 (MQ=255)

               aGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAg                                                                          <  2:3819449/93‑1 (MQ=255)

               aGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAg                                                                          >  2:2647399/1‑93 (MQ=255)

                 aaTTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGcc                                                                        >  1:5080339/1‑93 (MQ=255)

                  aTTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCt                                                                       <  2:4282590/93‑1 (MQ=255)

                     gctgcgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCa                                                                    <  2:2233342/90‑1 (MQ=255)

                      ccGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCaa                                                                   >  2:1220437/1‑93 (MQ=255)

                       cgcgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAg                                                                  >  2:4355079/1‑93 (MQ=255)

                       cgcgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAg                                                                  >  1:295611/1‑93 (MQ=255)

                       cgcgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGACAACTATCATAACCTGCAAg                                                                  >  1:5463395/1‑93 (MQ=255)

                        gcgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAgg                                                                 >  2:3445992/1‑92 (MQ=255)

                        gcgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGt                                                                 <  2:2744298/93‑1 (MQ=255)

                        gcgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGt                                                                 <  2:4902848/93‑1 (MQ=255)

                           aaaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGc                                                              >  2:289465/1‑93 (MQ=255)

                           aaaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGc                                                              >  2:3350518/1‑93 (MQ=255)

                            aaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGcc                                                             <  1:3512044/93‑1 (MQ=255)

                            aaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGcc                                                             >  2:5483204/1‑93 (MQ=255)

                            aaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGcc                                                             >  2:3038833/1‑93 (MQ=255)

                               ggTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCggc                                                          >  1:1579978/1‑92 (MQ=255)

                                gTTGTGAGGTAAAAGCATCGACGTGGTACCCCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGgag                                                         <  2:2550587/93‑1 (MQ=255)

                                gTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGgag                                                         >  1:2339148/1‑93 (MQ=255)

                                  tgtgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGagag                                                       <  2:685223/93‑1 (MQ=255)

                                  tgtgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGagag                                                       <  1:4393072/93‑1 (MQ=255)

                                   gtgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGc                                                      >  1:2821065/1‑93 (MQ=255)

                                    tgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCg                                                     >  1:2064104/1‑93 (MQ=255)

                                    tgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCg                                                     <  2:137645/93‑1 (MQ=255)

                                     gAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGa                                                    <  1:2745893/93‑1 (MQ=255)

                                      aGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGGGaa                                                   >  1:2324740/1‑93 (MQ=255)

                                      aGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGaa                                                   <  2:3858660/93‑1 (MQ=255)

                                      aGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGaa                                                   >  1:4538193/1‑93 (MQ=255)

                                      aGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGaa                                                   <  2:1646547/93‑1 (MQ=255)

                                       ggTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAg                                                  <  2:1406100/93‑1 (MQ=255)

                                       ggTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAg                                                  >  2:5399111/1‑93 (MQ=255)

                                       ggTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAg                                                  >  1:4842542/1‑93 (MQ=255)

                                       ggTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAg                                                  <  1:833095/93‑1 (MQ=255)

                                       ggTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAg                                                  <  2:6282302/93‑1 (MQ=255)

                                        gTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAgg                                                 <  1:5536470/93‑1 (MQ=255)

                                         tAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAggt                                                <  2:5874205/93‑2 (MQ=255)

                                         tAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAggg                                                <  1:2109920/93‑1 (MQ=255)

                                          aaaaGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGc                                               >  1:1441471/1‑93 (MQ=255)

                                          aaaaGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGc                                               >  1:4181061/1‑93 (MQ=255)

                                             aGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAATATCATAGCCTGCAAGTGGACGGAGAGCGAAGGGCTAt                                            >  1:2086204/1‑93 (MQ=255)

                                              gCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGCACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATc                                           >  2:1500684/1‑93 (MQ=255)

                                              gCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATc                                           >  1:3610616/1‑93 (MQ=255)

                                                aTCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCg                                         >  2:3518167/1‑93 (MQ=255)

                                                aTCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCg                                         <  2:2399259/93‑1 (MQ=255)

                                                aTCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCg                                         <  1:873434/93‑1 (MQ=255)

                                                aTCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCg                                         >  1:3756369/1‑93 (MQ=255)

                                                  cGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGc                                       <  2:3697566/93‑1 (MQ=255)

                                                   gACGTGTTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGcc                                      <  2:5896162/93‑1 (MQ=255)

                                                   gACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGGACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGcc                                      >  1:4311308/1‑93 (MQ=255)

                                                    aCGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCa                                     >  1:1962216/1‑93 (MQ=255)

                                                     cGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAg                                    >  2:2968616/1‑93 (MQ=255)

                                                       tGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAggg                                  >  2:1531119/1‑93 (MQ=255)

                                                        ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAgggg                                 >  1:3151594/1‑92 (MQ=255)

                                                        ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGt                                 <  2:4737338/93‑1 (MQ=255)

                                                        ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGt                                 <  2:5578862/93‑1 (MQ=255)

                                                        ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGt                                 <  1:6150453/93‑1 (MQ=255)

                                                        ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGt                                 >  1:5335375/1‑93 (MQ=255)

                                                          tACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGa                               <  2:1828024/93‑1 (MQ=255)

                                                          tACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGa                               >  1:5585846/1‑93 (MQ=255)

                                                           acacCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGaa                              >  1:188752/1‑93 (MQ=255)

                                                            cacCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGaaa                             <  2:5150590/93‑1 (MQ=255)

                                                              ccTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAAtt                           <  2:1816245/93‑1 (MQ=255)

                                                              ccTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAAtt                           <  2:5736710/93‑1 (MQ=255)

                                                                 gCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATc                        >  2:6436408/1‑93 (MQ=255)

                                                                    gTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcgcc                     <  1:528091/93‑1 (MQ=255)

                                                                    gTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcgcc                     <  1:6024101/93‑1 (MQ=255)

                                                                    gTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcgcc                     >  1:5052337/1‑93 (MQ=255)

                                                                      ttCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGTGTGAAATTATcgccgc                   <  2:4143125/93‑1 (MQ=255)

                                                                       tCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGGAATTATCGCcgcg                  >  1:1304987/1‑93 (MQ=255)

                                                                         aTTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGaa                >  1:2644088/1‑93 (MQ=255)

                                                                         aTTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATAGGCGGGaa                >  1:5960368/1‑93 (MQ=255)

                                                                          ttAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAAc               >  2:4038442/1‑93 (MQ=255)

                                                                           tAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACg              >  2:1946390/1‑93 (MQ=255)

                                                                            aaCGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGc             <  1:5418832/93‑1 (MQ=255)

                                                                            aaCGTGCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGc             >  1:6470542/1‑93 (MQ=255)

                                                                              cGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGcac           <  2:4486087/93‑1 (MQ=255)

                                                                              cGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGcac           >  2:200640/1‑93 (MQ=255)

                                                                               gTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGcaca          >  1:1833656/1‑93 (MQ=255)

                                                                                ttCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACaa         >  1:4422083/1‑93 (MQ=255)

                                                                                ttCTCCTGTGGCTGGAGAACTATCATAGCCTGAAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACaa         <  2:879137/93‑1 (MQ=255)

                                                                                 tctcCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAt        <  1:328163/93‑1 (MQ=255)

                                                                                 tctcCTGTGGCTGGAGAACGATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAt        <  1:2194670/93‑1 (MQ=255)

                                                                                   tcCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAttt      <  2:6223894/93‑1 (MQ=255)

                                                                                   tcCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAttt      >  2:5112318/1‑93 (MQ=255)

                                                                                     cTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCTCCGCGAACGCACAATTTGa    >  1:3017398/1‑93 (MQ=255)

                                                                                       gtgGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGata  >  1:2374485/1‑93 (MQ=255)

GAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATA  >  NC_000913/1533808‑1533987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGA  >  NC_000913/1533805‑1533991

GGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGG                                                                                               >  SRR4302534.1339940/1‑94 (MQ=60)

GGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGG                                                                                               >  SRR4302534.4359600/1‑94 (MQ=60)

 GGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGTTAAAAGCATCGACGTGGTACACCTGCGGATTCAGTATCGTTCTCCGGTGGC                                                                                              >  SRR4302534.6649997/1‑94 (MQ=60)

   GAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGTCTG                                                                                            >  SRR4302534.614554/1‑94 (MQ=60)

    AAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGG                                                                                           >  SRR4302534.4531127/1‑94 (MQ=60)

     AAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGA                                                                                          >  SRR4302534.5898744/1‑94 (MQ=60)

     AAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGA                                                                                          <  SRR4302534.3466013/94‑1 (MQ=60)

      AACACCACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAG                                                                                         <  SRR4302534.1538824/94‑1 (MQ=60)

       ACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGA                                                                                        <  SRR4302534.2932526/94‑1 (MQ=60)

        CAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAA                                                                                       >  SRR4302534.1506760/1‑94 (MQ=60)

        CAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAA                                                                                       >  SRR4302534.3171700/1‑94 (MQ=60)

        CAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAA                                                                                       >  SRR4302534.1911447/1‑94 (MQ=60)

          agccgggaaaaatgGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACT                                                                                     <  SRR4302534.1342615/80‑1 (MQ=60)

          ACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACT                                                                                     <  SRR4302534.140248/94‑1 (MQ=60)

           CACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTA                                                                                    <  SRR4302534.3269775/94‑1 (MQ=60)

            ACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTAT                                                                                   >  SRR4302534.6066023/1‑94 (MQ=60)

             CCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATC                                                                                  <  SRR4302534.4846641/94‑1 (MQ=60)

              CGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCA                                                                                 >  SRR4302534.4497902/1‑94 (MQ=60)

              CGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCA                                                                                 <  SRR4302534.3759449/94‑1 (MQ=60)

               GGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCAT                                                                                <  SRR4302534.378913/94‑1 (MQ=60)

               GGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGCTCTCCTGTGGCTGGAaaaccatcct                                                                                >  SRR4302534.1076309/1‑84 (MQ=60)

                GCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATA                                                                               <  SRR4302534.346608/94‑1 (MQ=60)

                GCAGAATGGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATA                                                                               <  SRR4302534.5568633/94‑1 (MQ=60)

                 CAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAG                                                                              <  SRR4302534.4894131/94‑1 (MQ=60)

                 CAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAG                                                                              <  SRR4302534.6242834/94‑1 (MQ=60)

                 CAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAG                                                                              >  SRR4302534.5216128/1‑94 (MQ=60)

                 CAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAG                                                                              >  SRR4302534.5322857/1‑94 (MQ=60)

                 CAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAG                                                                              >  SRR4302534.2259039/1‑94 (MQ=60)

                  AGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGCTTCATTAACGATCTCCTGTTGCTGGAGGACTATCATAGC                                                                             >  SRR4302534.2420256/1‑94 (MQ=60)

                   GAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCC                                                                            >  SRR4302534.5217470/1‑94 (MQ=60)

                     ATTGCCGCGAAACGGTTGTGAGTTAAAAGCATCGACGTGGTACACCTGCGATTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTG                                                                          <  SRR4302534.2027579/94‑1 (MQ=60)

                      TTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGC                                                                         <  SRR4302534.4593124/94‑1 (MQ=60)

                         CCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGTGAACTATTATAGCCTGAAAG                                                                      >  SRR4302534.5334418/1‑94 (MQ=60)

                         CCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAG                                                                      >  SRR4302534.303257/1‑94 (MQ=60)

                         CCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGACAACTATCATAACCTGCAAG                                                                      >  SRR4302534.5610654/1‑94 (MQ=60)

                           GCGAATCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                    <  SRR4302534.6119639/94‑1 (MQ=60)

                           GCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                    <  SRR4302534.238789/94‑1 (MQ=60)

                           GCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                    <  SRR4302534.3586291/94‑1 (MQ=60)

                           GCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                    <  SRR4302534.4486796/94‑1 (MQ=60)

                            CTAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTATCGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGG                                                                   <  SRR4302534.6176360/94‑1 (MQ=60)

                            CGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGG                                                                   >  SRR4302534.1862725/1‑94 (MQ=60)

                              AAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCC                                                                 >  SRR4302534.931872/1‑94 (MQ=60)

                              AAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCC                                                                 >  SRR4302534.2826579/1‑94 (MQ=60)

                               AACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCG                                                                <  SRR4302534.3606516/94‑1 (MQ=60)

                               AACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCG                                                                >  SRR4302534.3783333/1‑94 (MQ=60)

                                 CGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGC                                                              >  SRR4302534.1621312/1‑94 (MQ=60)

                                  GGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAG                                                             >  SRR4302534.2401224/1‑94 (MQ=60)

                                   GTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGA                                                            <  SRR4302534.874320/94‑1 (MQ=60)

                                   GTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGA                                                            <  SRR4302534.667908/94‑1 (MQ=60)

                                   GTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGA                                                            <  SRR4302534.791391/94‑1 (MQ=60)

                                    TTGTTAGTTAAAATCATCGACGTGGTACTCCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAG                                                           <  SRR4302534.2274754/94‑1 (MQ=60)

                                     TGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGC                                                          >  SRR4302534.2895239/1‑94 (MQ=60)

                                     TGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGC                                                          <  SRR4302534.4511551/94‑1 (MQ=60)

                                      GTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCG                                                         >  SRR4302534.2118075/1‑94 (MQ=60)

                                       TGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGACGGAGAGCCA                                                        >  SRR4302534.2159500/1‑94 (MQ=60)

                                        GAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGGGAA                                                       >  SRR4302534.2386425/1‑94 (MQ=60)

                                        GAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAA                                                       >  SRR4302534.4661192/1‑94 (MQ=60)

                                        GAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAA                                                       <  SRR4302534.2818214/94‑1 (MQ=60)

                                         AGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAG                                                      >  SRR4302534.4973931/1‑94 (MQ=60)

                                          GGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGG                                                     <  SRR4302534.854051/94‑1 (MQ=60)

                                           GTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGG                                                    <  SRR4302534.4698595/94‑1 (MQ=60)

                                           GTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGG                                                    <  SRR4302534.5685920/94‑1 (MQ=60)

                                            TAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGC                                                   >  SRR4302534.3089818/1‑94 (MQ=60)

                                            TAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGC                                                   >  SRR4302534.4294033/1‑94 (MQ=60)

                                            TAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGC                                                   >  SRR4302534.1981738/1‑94 (MQ=60)

                                            TAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGC                                                   >  SRR4302534.1479405/1‑94 (MQ=60)

                                            TAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGC                                                   <  SRR4302534.2165382/94‑1 (MQ=60)

                                             AAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCT                                                  <  SRR4302534.1534508/94‑1 (MQ=60)

                                             AAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCT                                                  >  SRR4302534.2896128/1‑94 (MQ=60)

                                             AAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCT                                                  >  SRR4302534.4631823/1‑94 (MQ=60)

                                               AAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAATATCATAGCCTGCAAGTGGACGGAGAGCGAAGGGCTAT                                                >  SRR4302534.2140878/1‑94 (MQ=60)

                                                AGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATC                                               >  SRR4302534.3707708/1‑94 (MQ=60)

                                                  CATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCG                                             >  SRR4302534.3857228/1‑94 (MQ=60)

                                                  CATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCG                                             <  SRR4302534.3075980/94‑1 (MQ=60)

                                                   ATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGG                                            >  SRR4302534.989403/1‑94 (MQ=60)

                                                   ATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGG                                            <  SRR4302534.895516/94‑1 (MQ=60)

                                                   ATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGG                                            <  SRR4302534.4686106/94‑1 (MQ=60)

                                                    TCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGC                                           <  SRR4302534.5066504/94‑1 (MQ=60)

                                                     CGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGGACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCC                                          >  SRR4302534.4428111/1‑94 (MQ=60)

                                                     CGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCC                                          >  SRR4302534.2225657/1‑94 (MQ=60)

                                                     CGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCC                                          >  SRR4302534.5653621/1‑94 (MQ=60)

                                                      GACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCA                                         >  SRR4302534.2013576/1‑94 (MQ=60)

                                                      GACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCA                                         <  SRR4302534.345373/94‑1 (MQ=60)

                                                      GACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCA                                         >  SRR4302534.6522809/1‑94 (MQ=60)

                                                         GTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGG                                      >  SRR4302534.5532480/1‑94 (MQ=60)

                                                         GTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGG                                      >  SRR4302534.5465833/1‑94 (MQ=60)

                                                          TGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGT                                     >  SRR4302534.5479240/1‑94 (MQ=60)

                                                          TGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGG                                     >  SRR4302534.3234567/1‑94 (MQ=60)

                                                           GGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTG                                    >  SRR4302534.1160487/1‑94 (MQ=60)

                                                           GGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTG                                    <  SRR4302534.2084741/94‑1 (MQ=60)

                                                           GGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTG                                    <  SRR4302534.6317775/94‑1 (MQ=60)

                                                           GGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTG                                    >  SRR4302534.3669503/1‑94 (MQ=60)

                                                           GGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAACCCCCAAGTGGCCGGAGAGCGAAGGGGTATCCGGCCAGGGGG                                    >  SRR4302534.4685851/1‑94 (MQ=60)

                                                            GTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGA                                   >  SRR4302534.5736804/1‑94 (MQ=60)

                                                            GTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGA                                   >  SRR4302534.2577578/1‑94 (MQ=60)

                                                             TACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAA                                  <  SRR4302534.1880510/94‑1 (MQ=60)

                                                             TACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAA                                  >  SRR4302534.193715/1‑94 (MQ=60)

                                                             TACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAA                                  >  SRR4302534.149878/1‑94 (MQ=60)

                                                             TACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGCCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAA                                  <  SRR4302534.2411407/94‑1 (MQ=60)

                                                                ACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATT                               <  SRR4302534.2125893/94‑1 (MQ=60)

                                                                 CCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTA                              >  SRR4302534.36844/1‑94 (MQ=60)

                                                                 CCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGGGAAATTA                              >  SRR4302534.215225/1‑94 (MQ=60)

                                                                  CTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTAT                             >  SRR4302534.3729413/1‑94 (MQ=60)

                                                                  CTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTAT                             >  SRR4302534.4394517/1‑94 (MQ=60)

                                                                   TGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATC                            <  SRR4302534.5479103/94‑1 (MQ=60)

                                                                      GGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCC                         >  SRR4302534.2782041/1‑94 (MQ=60)

                                                                      GGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCC                         >  SRR4302534.5188780/1‑94 (MQ=60)

                                                                      GGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCC                         >  SRR4302534.5001539/1‑94 (MQ=60)

                                                                       GTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCG                        <  SRR4302534.541196/94‑1 (MQ=60)

                                                                       GTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCG                        <  SRR4302534.3240564/94‑1 (MQ=60)

                                                                       GTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCG                        <  SRR4302534.6287159/94‑1 (MQ=60)

                                                                       GTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCG                        <  SRR4302534.6187816/94‑1 (MQ=60)

                                                                        TTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGGGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGC                       >  SRR4302534.5567077/1‑94 (MQ=60)

                                                                         tccaaTAACGTTCTCCTGAGCCTGGAGCACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCG                      <  SRR4302534.1653231/89‑1 (MQ=60)

                                                                         TTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGGAATTATCGCCGCG                      >  SRR4302534.1339412/1‑94 (MQ=60)

                                                                         TTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCG                      <  SRR4302534.4245374/94‑1 (MQ=60)

                                                                         TTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCG                      <  SRR4302534.2888030/94‑1 (MQ=60)

                                                                         TTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCG                      <  SRR4302534.2129647/94‑1 (MQ=60)

                                                                           CATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATaggcgggaa                    >  SRR4302534.6122199/1‑85 (MQ=60)

                                                                           CATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAA                    >  SRR4302534.2714006/1‑94 (MQ=60)

                                                                            ATTACCGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGGGGCCGTAGAGCGAGGGGTCATCCGGCCAGGGTGAAATTATCGCCGCGAAC                   <  SRR4302534.36485/94‑1 (MQ=60)

                                                                            ATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAAC                   >  SRR4302534.5710089/1‑94 (MQ=60)

                                                                              TAACGTGCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGC                 >  SRR4302534.6644831/1‑94 (MQ=60)

                                                                               AACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGTGAACGCA                <  SRR4302534.2962043/94‑1 (MQ=60)

                                                                               AACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCA                <  SRR4302534.5564633/94‑1 (MQ=60)

                                                                                ACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCAC               <  SRR4302534.3940622/94‑1 (MQ=60)

                                                                                 CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCCCA              >  SRR4302534.1958802/1‑94 (MQ=60)

                                                                                 CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACA              >  SRR4302534.5872491/1‑94 (MQ=60)

                                                                                 CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACA              >  SRR4302534.1881332/1‑94 (MQ=60)

                                                                                  GTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAA             <  SRR4302534.6161702/94‑1 (MQ=60)

                                                                                  GTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAA             >  SRR4302534.4541477/1‑94 (MQ=60)

                                                                                   TTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAT            <  SRR4302534.2960645/94‑1 (MQ=60)

                                                                                    TCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATT           <  SRR4302534.336597/94‑1 (MQ=60)

                                                                                    TCTCCTGTGGCTGGAGAACGATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATT           <  SRR4302534.2252726/94‑1 (MQ=60)

                                                                                      TCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTG         <  SRR4302534.1434101/94‑1 (MQ=60)

                                                                                       CCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCTCCGCGAACGCACAATTTGA        >  SRR4302534.3096390/1‑94 (MQ=60)

                                                                                        CTGTGGCTGGAGAACTATCATATCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGAT       <  SRR4302534.5653167/94‑1 (MQ=60)

                                                                                         TGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATA      >  SRR4302534.2437543/1‑94 (MQ=60)

                                                                                           TGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATC    <  SRR4302534.3011197/94‑1 (MQ=60)

                                                                                             GCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGA  >  SRR4302534.50787/1‑94 (MQ=60)

                                                                                             GCTGGAGAACTATCATAGCCTGCAAGTGACCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGA  <  SRR4302534.6066885/94‑1 (MQ=60)

GGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGA  >  NC_000913/1533805‑1533991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`