Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,533,898 A→G intergenic (‑16/+63) yddE ← / ← narV hypothetical protein/cryptic nitrate reductase 2 gamma subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,533,8980AG99.3% 1022.7 / ‑3.3 298intergenic (‑16/+63)yddE/narVhypothetical protein/cryptic nitrate reductase 2 gamma subunit
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (148/148);  total (150/148)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 304 total aligned reads displayed.

GAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATA  >  NC_000913/1533808‑1533987
                                                                                          |                                                                                         
 aaaaCAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTg                                                                                        <  1:3382518/93‑1 (MQ=255)
  aaaCAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTgg                                                                                       >  1:7083385/1‑93 (MQ=255)
   aacaacACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGga                                                                                      >  2:3156759/1‑93 (MQ=255)
   aacaacACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGga                                                                                      <  2:1632552/93‑1 (MQ=255)
   aacaacACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGga                                                                                      >  2:1196886/1‑93 (MQ=255)
   aacaacACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGga                                                                                      <  1:6951809/93‑1 (MQ=255)
    acaacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTgggg                                                                                     >  2:5373309/1‑91 (MQ=255)
    acaacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTggcc                                                                                     >  1:2833168/1‑91 (MQ=255)
    acaacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGgag                                                                                     >  2:2343973/1‑93 (MQ=255)
    acaacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGgag                                                                                     >  1:3941802/1‑93 (MQ=255)
     caacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGgaga                                                                                    >  2:1544086/1‑93 (MQ=255)
      aacaCCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGaa                                                                                   <  1:4319403/93‑1 (MQ=255)
       acacCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAc                                                                                  >  1:30421/1‑93 (MQ=255)
         acCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTa                                                                                <  1:1232009/93‑1 (MQ=255)
          ccGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTAt                                                                               <  1:384349/93‑1 (MQ=255)
            ggCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCa                                                                             <  2:6153183/93‑1 (MQ=255)
             gCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCAt                                                                            <  2:2784967/93‑1 (MQ=255)
             gCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCAt                                                                            >  2:8069443/1‑93 (MQ=255)
                gAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGc                                                                         <  2:3000236/93‑1 (MQ=255)
                gAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGc                                                                         >  1:4624498/1‑93 (MQ=255)
                gAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGc                                                                         <  2:3580168/93‑1 (MQ=255)
                gAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGc                                                                         >  1:5242381/1‑93 (MQ=255)
                 aaTTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGcc                                                                        >  2:4185704/1‑93 (MQ=255)
                  aTTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCt                                                                       <  1:7493001/93‑1 (MQ=255)
                  aTTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCt                                                                       <  1:4044104/93‑1 (MQ=255)
                  aTTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCt                                                                       <  1:32951/93‑1 (MQ=255)
                   ttGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTg                                                                      <  1:5128774/93‑1 (MQ=255)
                    tGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGc                                                                     <  2:7769460/93‑1 (MQ=255)
                     gCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCa                                                                    <  2:8504526/93‑1 (MQ=255)
                     gCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCa                                                                    <  1:6072926/93‑1 (MQ=255)
                     gCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCa                                                                    >  2:1879599/1‑93 (MQ=255)
                      ccGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCaa                                                                   <  2:3286467/93‑1 (MQ=255)
                        gcgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGt                                                                 <  1:6795287/93‑1 (MQ=255)
                        ccgAAACGGTTG‑GAAGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTg                                                                <  1:8449670/92‑1 (MQ=255)
                        cagAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGt                                                                 <  1:6144689/91‑1 (MQ=255)
                         cgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTg                                                                <  1:4959913/93‑1 (MQ=255)
                         cgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTg                                                                <  2:5621215/93‑1 (MQ=255)
                         cgAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTg                                                                <  2:7253041/93‑1 (MQ=255)
                           aaaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGc                                                              <  2:4866597/93‑1 (MQ=255)
                           aaaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGc                                                              >  2:2875240/1‑93 (MQ=255)
                           aaaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGc                                                              >  2:3442189/1‑93 (MQ=255)
                           aaaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGc                                                              <  2:7889117/93‑1 (MQ=255)
                            aaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGcc                                                             >  2:5324078/1‑93 (MQ=255)
                            aaCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGcc                                                             >  2:6385065/1‑93 (MQ=255)
                                  tgtgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGagag                                                       <  1:6854594/93‑1 (MQ=255)
                                  tgtgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGagag                                                       <  1:8613495/93‑1 (MQ=255)
                                  tgtgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGagag                                                       <  1:4679009/93‑1 (MQ=255)
                                    tgAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCg                                                     <  2:2452092/93‑1 (MQ=255)
                                     gAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGGGCGa                                                    >  1:6399511/1‑93 (MQ=255)
                                     gAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGa                                                    <  2:5098160/93‑1 (MQ=255)
                                      aGGTAAAAGCATCGGCGTTGTACGCCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAATTATCATAGCCTGCAAGTGGCCGGAGAGCGaa                                                   <  2:2696967/93‑1 (MQ=255)
                                       ggTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAg                                                  <  1:5515440/93‑1 (MQ=255)
                                        gTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAgg                                                 <  1:801457/93‑1 (MQ=255)
                                         tAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAggg                                                >  1:901010/1‑93 (MQ=255)
                                          aaaaGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGc                                               <  1:8254951/93‑1 (MQ=255)
                                          aaaaGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGc                                               <  2:5787180/93‑1 (MQ=255)
                                          aaaaGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGGGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGc                                               <  2:1785686/93‑1 (MQ=255)
                                            aaGCATCGACGTGGTACACCTGCGGTTTCATTAACGTACTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTa                                             <  1:2431945/93‑1 (MQ=255)
                                             aGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTAt                                            <  2:2246185/93‑1 (MQ=255)
                                               cATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATcc                                          <  1:6667712/93‑1 (MQ=255)
                                               cATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATcc                                          <  2:7471082/93‑1 (MQ=255)
                                               cATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATcc                                          <  2:6045006/93‑1 (MQ=255)
                                                aTCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCg                                         >  1:1954162/1‑93 (MQ=255)
                                                   gACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCCGcc                                      >  2:6502704/1‑93 (MQ=255)
                                                    aCGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGTAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCa                                     >  1:5430125/1‑93 (MQ=255)
                                                       tGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAggg                                  <  1:7667952/93‑1 (MQ=255)
                                                        ggTACCCCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGt                                 <  1:5672349/93‑1 (MQ=255)
                                                        ggTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAgggg                                 >  1:8098418/1‑92 (MQ=255)
                                                         gTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTg                                <  2:6701636/93‑1 (MQ=255)
                                                           acacCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGGTATCCGGCCAGGGTGaa                              >  2:4327180/1‑93 (MQ=255)
                                                            cacCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGTACTATCATAGCCTGCAAGTGGCCGGAGGGCGAAGGGCTTTCCGGCCAGGGAGaaa                             >  2:8578275/1‑93 (MQ=255)
                                                            cacCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGaaa                             <  2:7054407/93‑1 (MQ=255)
                                                             acCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAAt                            >  1:345737/1‑93 (MQ=255)
                                                             acCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAAt                            >  2:8446928/1‑93 (MQ=255)
                                                              ccTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGTGAGCGAAGGGCTATCCGGCCAGGGTGAAAtt                           >  1:3187368/1‑93 (MQ=255)
                                                              ccTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGCGCGAAGGGGTATCCGGCCAGGGGGAAAtt                           >  1:1694589/1‑93 (MQ=255)
                                                              ccTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAAtt                           >  2:2618771/1‑93 (MQ=255)
                                                                tGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTAt                         >  1:6311691/1‑93 (MQ=255)
                                                                tGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTAt                         <  1:7578136/93‑1 (MQ=255)
                                                                    gTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCTGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcgcc                     <  2:3073035/93‑1 (MQ=255)
                                                                    gTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcgcc                     >  1:2366617/1‑93 (MQ=255)
                                                                    gTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATcgcc                     >  2:3654530/1‑93 (MQ=255)
                                                                       tCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGGCAGGGTGAAATTATCGCcgcg                  >  2:8284839/1‑93 (MQ=255)
                                                                       tCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCcgcg                  >  2:889224/1‑93 (MQ=255)
                                                                         cttAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGaa                <  1:2072347/92‑1 (MQ=255)
                                                                          ttAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAAc               <  2:3889391/93‑1 (MQ=255)
                                                                             aCGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGca            >  1:1380329/1‑93 (MQ=255)
                                                                              cGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGcac           <  1:6456560/93‑1 (MQ=255)
                                                                               gTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGcaca          >  2:6241557/1‑93 (MQ=255)
                                                                               gTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGcaca          >  1:4460476/1‑93 (MQ=255)
                                                                                ttCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACaa         <  1:4773457/93‑1 (MQ=255)
                                                                                 tctcCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGGCGCGCACCCACAAt        >  2:1452498/1‑93 (MQ=255)
                                                                                 tctcCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAt        <  1:7287157/93‑1 (MQ=255)
                                                                                   tcCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAttt      >  2:7199771/1‑93 (MQ=255)
                                                                                   tcCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAttt      <  2:4411391/93‑1 (MQ=255)
                                                                                   tcCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAttt      >  2:4968193/1‑93 (MQ=255)
                                                                                     cTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGa    <  1:2631847/93‑1 (MQ=255)
                                                                                     cTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGa    >  2:4620576/1‑93 (MQ=255)
                                                                                      tgtgGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGat   <  1:1050280/93‑1 (MQ=255)
                                                                                       gtgGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGata  <  1:738583/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATA  >  NC_000913/1533808‑1533987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCG  >  NC_000913/1533806‑1533990
                                                                                            |                                                                                            
GGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGC                                                                                             >  SRR4302541.8012644/1‑94 (MQ=60)
GGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGC                                                                                             <  SRR4302541.4403599/94‑1 (MQ=60)
 GGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCT                                                                                            >  SRR4302541.278141/1‑94 (MQ=60)
 GGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCT                                                                                            <  SRR4302541.7496703/94‑1 (MQ=60)
 GGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCT                                                                                            <  SRR4302541.594661/94‑1 (MQ=60)
  GAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTG                                                                                           <  SRR4302541.725723/94‑1 (MQ=60)
   AAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGG                                                                                          <  SRR4302541.3416237/94‑1 (MQ=60)
   AAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGG                                                                                          >  SRR4302541.7433155/1‑94 (MQ=60)
   AAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGG                                                                                          >  SRR4302541.7158728/1‑94 (MQ=60)
    AAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGA                                                                                         <  SRR4302541.4869456/94‑1 (MQ=60)
     AACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAG                                                                                        >  SRR4302541.3981020/1‑94 (MQ=60)
     AACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAG                                                                                        <  SRR4302541.7025950/94‑1 (MQ=60)
     AACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAG                                                                                        <  SRR4302541.4498555/94‑1 (MQ=60)
        AACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAC                                                                                     >  SRR4302541.8820874/1‑94 (MQ=60)
        AACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAC                                                                                     >  SRR4302541.30664/1‑94 (MQ=60)
        AACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAC                                                                                     <  SRR4302541.4362533/94‑1 (MQ=60)
        AACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAAC                                                                                     <  SRR4302541.764168/94‑1 (MQ=60)
           ACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTAT                                                                                  <  SRR4302541.1244129/94‑1 (MQ=60)
           ACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTAT                                                                                  <  SRR4302541.8524344/94‑1 (MQ=60)
            CCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATC                                                                                 <  SRR4302541.4791136/94‑1 (MQ=60)
            CCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATC                                                                                 <  SRR4302541.2329456/94‑1 (MQ=60)
            CCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATC                                                                                 <  SRR4302541.387896/94‑1 (MQ=60)
              GGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCAT                                                                               >  SRR4302541.6473711/1‑94 (MQ=60)
                CAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAG                                                                             >  SRR4302541.6864055/1‑94 (MQ=60)
                 AGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGC                                                                            >  SRR4302541.5296401/1‑94 (MQ=60)
                 AGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGC                                                                            >  SRR4302541.4671528/1‑94 (MQ=60)
                 AGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGC                                                                            >  SRR4302541.4671526/1‑94 (MQ=60)
                 AGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGC                                                                            >  SRR4302541.4511686/1‑94 (MQ=60)
                  GAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCC                                                                           >  SRR4302541.5309782/1‑94 (MQ=60)
                  GAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCC                                                                           >  SRR4302541.3176208/1‑94 (MQ=60)
                  GAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCC                                                                           <  SRR4302541.1176616/94‑1 (MQ=60)
                   AATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCT                                                                          <  SRR4302541.4069741/94‑1 (MQ=60)
                   AATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCT                                                                          <  SRR4302541.3474164/94‑1 (MQ=60)
                    ATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTG                                                                         >  SRR4302541.1701866/1‑94 (MQ=60)
                    ATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTG                                                                         <  SRR4302541.7573124/94‑1 (MQ=60)
                    ATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTG                                                                         <  SRR4302541.4084026/94‑1 (MQ=60)
                    ATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTG                                                                         <  SRR4302541.33208/94‑1 (MQ=60)
                     TTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGC                                                                        <  SRR4302541.5181498/94‑1 (MQ=60)
                       GCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAA                                                                      <  SRR4302541.6134930/94‑1 (MQ=60)
                         CGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGT                                                                    >  SRR4302541.6142958/1‑94 (MQ=60)
                          caGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                   <  SRR4302541.6207459/92‑1 (MQ=60)
                          GCGAAGCGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                   <  SRR4302541.3406811/94‑1 (MQ=60)
                          GCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                   >  SRR4302541.3412032/1‑94 (MQ=60)
                          GCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                   >  SRR4302541.2439570/1‑94 (MQ=60)
                          GCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTG                                                                   <  SRR4302541.6867958/94‑1 (MQ=60)
                          CCGAAACGGTTG‑GAAGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGG                                                                  <  SRR4302541.8540883/94‑1 (MQ=60)
                           CTAAAAGGTTTTGAGGTAAAAGCATCGACGTTGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGTTGGAGAACTATCATAGCCTGCAAGTGG                                                                  <  SRR4302541.7112408/94‑1 (MQ=60)
                           CGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGG                                                                  <  SRR4302541.5314763/94‑1 (MQ=60)
                           CGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGG                                                                  >  SRR4302541.7092202/1‑94 (MQ=60)
                           CGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGG                                                                  <  SRR4302541.5010674/94‑1 (MQ=60)
                           CGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGG                                                                  >  SRR4302541.8226516/1‑94 (MQ=60)
                           CGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGG                                                                  <  SRR4302541.944773/94‑1 (MQ=60)
                            GAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGC                                                                 <  SRR4302541.2189440/94‑1 (MQ=60)
                             AAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCC                                                                <  SRR4302541.259842/94‑1 (MQ=60)
                             AAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCC                                                                >  SRR4302541.2955551/1‑94 (MQ=60)
                                CGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGA                                                             >  SRR4302541.7859228/1‑94 (MQ=60)
                                 GGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAG                                                            >  SRR4302541.4228168/1‑94 (MQ=60)
                                  GTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGA                                                           <  SRR4302541.3426901/94‑1 (MQ=60)
                                   TTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAG                                                          <  SRR4302541.438303/94‑1 (MQ=60)
                                   TTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAG                                                          <  SRR4302541.624429/94‑1 (MQ=60)
                                   TTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAG                                                          <  SRR4302541.85045/94‑1 (MQ=60)
                                    TGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGC                                                         >  SRR4302541.515577/1‑94 (MQ=60)
                                    TGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGC                                                         <  SRR4302541.6927853/94‑1 (MQ=60)
                                    TGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGC                                                         <  SRR4302541.8705859/94‑1 (MQ=60)
                                    TGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGC                                                         <  SRR4302541.4726668/94‑1 (MQ=60)
                                     GTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCG                                                        <  SRR4302541.393529/94‑1 (MQ=60)
                                      TGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGGGCGA                                                       >  SRR4302541.6464820/1‑94 (MQ=60)
                                      TGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGA                                                       <  SRR4302541.7765055/94‑1 (MQ=60)
                                        AGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAG                                                     <  SRR4302541.2902471/94‑1 (MQ=60)
                                         GGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGGACTATCATAGCCTGCCAGTGGCCGGAGAGCGAAGG                                                    >  SRR4302541.5503678/1‑94 (MQ=60)
                                         GGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGG                                                    <  SRR4302541.5729490/94‑1 (MQ=60)
                                         GGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGG                                                    <  SRR4302541.5571899/94‑1 (MQ=60)
                                         GGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGG                                                    <  SRR4302541.4239133/94‑1 (MQ=60)
                                          GTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGG                                                   >  SRR4302541.6213563/1‑94 (MQ=60)
                                          GTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGG                                                   <  SRR4302541.697922/94‑1 (MQ=60)
                                          GTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGG                                                   >  SRR4302541.909364/1‑94 (MQ=60)
                                          GTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGG                                                   >  SRR4302541.2683383/1‑94 (MQ=60)
                                          GTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGG                                                   <  SRR4302541.808825/94‑1 (MQ=60)
                                           TAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGGGCGAAGGGG                                                  >  SRR4302541.1735814/1‑94 (MQ=60)
                                           TAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGC                                                  >  SRR4302541.386431/1‑94 (MQ=60)
                                            AAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCT                                                 <  SRR4302541.5262464/94‑1 (MQ=60)
                                            AAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCT                                                 <  SRR4302541.1214002/94‑1 (MQ=60)
                                            AAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCT                                                 <  SRR4302541.8344846/94‑1 (MQ=60)
                                            AAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCT                                                 <  SRR4302541.2005002/94‑1 (MQ=60)
                                             AAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTA                                                <  SRR4302541.6691072/94‑1 (MQ=60)
                                             AAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTA                                                >  SRR4302541.6509001/1‑94 (MQ=60)
                                              AAGCATCGACGTGGTACCCCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTAT                                               <  SRR4302541.6418887/94‑1 (MQ=60)
                                              AAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTAT                                               >  SRR4302541.7391220/1‑94 (MQ=60)
                                              AAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTACTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTAT                                               <  SRR4302541.2456018/94‑1 (MQ=60)
                                               AGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATC                                              >  SRR4302541.3683816/1‑94 (MQ=60)
                                                 CATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCG                                            >  SRR4302541.3228962/1‑94 (MQ=60)
                                                 CATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCG                                            <  SRR4302541.6736183/94‑1 (MQ=60)
                                                 CATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCG                                            <  SRR4302541.2402159/94‑1 (MQ=60)
                                                 CATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCG                                            >  SRR4302541.1972994/1‑94 (MQ=60)
                                                  ATCGACGTGGTCCACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGG                                           >  SRR4302541.6418481/1‑94 (MQ=60)
                                                   TCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGC                                          >  SRR4302541.3979934/1‑94 (MQ=60)
                                                   TCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGC                                          >  SRR4302541.6804535/1‑94 (MQ=60)
                                                    CGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCC                                         <  SRR4302541.501390/94‑1 (MQ=60)
                                                    CGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATACCGCC                                         >  SRR4302541.8152445/1‑94 (MQ=60)
                                                     GACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGTAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCA                                        >  SRR4302541.5485836/1‑94 (MQ=60)
                                                     GACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCA                                        >  SRR4302541.1362634/1‑94 (MQ=60)
                                                     GACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCA                                        <  SRR4302541.5224548/94‑1 (MQ=60)
                                                     GACGGTGTACCCCTGCGGTTTCATTACCGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCA                                        <  SRR4302541.5224546/94‑1 (MQ=60)
                                                       CGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGG                                      <  SRR4302541.7714453/94‑1 (MQ=60)
                                                        GTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGG                                     <  SRR4302541.6052779/94‑1 (MQ=60)
                                                         TGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGT                                    <  SRR4302541.7749515/94‑1 (MQ=60)
                                                         TGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGT                                    <  SRR4302541.688444/94‑1 (MQ=60)
                                                         TGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGG                                    >  SRR4302541.8187195/1‑94 (MQ=60)
                                                          GGTACCCCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTG                                   <  SRR4302541.5730242/94‑1 (MQ=60)
                                                          GGTACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTG                                   >  SRR4302541.4852950/1‑94 (MQ=60)
                                                            TATACCTGCGGTTTCATTAACGTACTCCTGTAGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAA                                 <  SRR4302541.5643513/94‑1 (MQ=60)
                                                            TACACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAA                                 >  SRR4302541.3252480/1‑94 (MQ=60)
                                                              CACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAAT                               >  SRR4302541.348847/1‑94 (MQ=60)
                                                              CACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGGGAAAT                               >  SRR4302541.6536047/1‑94 (MQ=60)
                                                               ACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGTGAGCGAAGGGCTATCCGGCCAGGGTGAAATT                              >  SRR4302541.3219567/1‑94 (MQ=60)
                                                               ACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGCGCGAAGGGGTATCCGGCCAGGGGGAAATT                              >  SRR4302541.1711214/1‑94 (MQ=60)
                                                               ACCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATT                              <  SRR4302541.1333697/94‑1 (MQ=60)
                                                                CCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTA                             >  SRR4302541.7706679/1‑94 (MQ=60)
                                                                CCTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTA                             >  SRR4302541.3198155/1‑94 (MQ=60)
                                                                 CTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTAT                            >  SRR4302541.6376222/1‑94 (MQ=60)
                                                                 CTGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTAT                            >  SRR4302541.8645555/1‑94 (MQ=60)
                                                                  TGCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATC                           <  SRR4302541.7659107/94‑1 (MQ=60)
                                                                   GCTGTTTCATTAACGTTCTCCTGTGGCTGTAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATTG                          <  SRR4302541.8793631/94‑1 (MQ=60)
                                                                   GCGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCG                          <  SRR4302541.7539282/94‑1 (MQ=60)
                                                                    CGGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGC                         >  SRR4302541.8574702/1‑94 (MQ=60)
                                                                     GGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCC                        <  SRR4302541.4698940/94‑1 (MQ=60)
                                                                     GGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCC                        >  SRR4302541.2389694/1‑94 (MQ=60)
                                                                     GGTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGAGCTATCCGGCCAGGGTGAAATTATCGCC                        <  SRR4302541.1906894/94‑1 (MQ=60)
                                                                      GTTTCATTAACGTTCTCCTGTGNCNGGAGNACTATCATANCCNGNANGTGGNCNGAGNGCGANGGngtnnnnnnncnngggnaannnntngnnn                       >  SRR4302541.6616060/1‑65 (MQ=56)
                                                                      GTTTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCG                       <  SRR4302541.7309186/94‑1 (MQ=60)
                                                                        TTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCG                     >  SRR4302541.5272348/1‑94 (MQ=60)
                                                                        TTCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCG                     <  SRR4302541.2532793/94‑1 (MQ=60)
                                                                         TCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGA                    >  SRR4302541.4075117/1‑94 (MQ=60)
                                                                         TCATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGGGAAATTATCGCCGCGA                    >  SRR4302541.5532998/1‑94 (MQ=60)
                                                                          CATTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAA                   >  SRR4302541.5934549/1‑94 (MQ=60)
                                                                           CTTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAAC                  <  SRR4302541.2092379/94‑1 (MQ=60)
                                                                            TTAACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACG                 >  SRR4302541.8159504/1‑94 (MQ=60)
                                                                              AACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCA               >  SRR4302541.1393888/1‑94 (MQ=60)
                                                                              AACGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCA               >  SRR4302541.6178682/1‑94 (MQ=60)
                                                                                CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACA             >  SRR4302541.4505381/1‑94 (MQ=60)
                                                                                CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACA             >  SRR4302541.1647224/1‑94 (MQ=60)
                                                                                CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACA             >  SRR4302541.958616/1‑94 (MQ=60)
                                                                                CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACA             <  SRR4302541.6522359/94‑1 (MQ=60)
                                                                                CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACA             >  SRR4302541.4505382/1‑94 (MQ=60)
                                                                                CGTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCCGCCAGGGGGAACTTATCGCCGCGAACTCACA             >  SRR4302541.7488816/1‑94 (MQ=60)
                                                                                 GTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAA            >  SRR4302541.4744783/1‑94 (MQ=60)
                                                                                 GTTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAA            >  SRR4302541.6896104/1‑94 (MQ=60)
                                                                                  TTCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAT           <  SRR4302541.4822198/94‑1 (MQ=60)
                                                                                   TCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATT          <  SRR4302541.7364921/94‑1 (MQ=60)
                                                                                   TCTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATT          >  SRR4302541.8284217/1‑94 (MQ=60)
                                                                                    CTCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTT         >  SRR4302541.205421/1‑94 (MQ=60)
                                                                                     TCCTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTG        <  SRR4302541.701403/94‑1 (MQ=60)
                                                                                       CTGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGAT      <  SRR4302541.2658096/94‑1 (MQ=60)
                                                                                        TGTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATA     <  SRR4302541.1060035/94‑1 (MQ=60)
                                                                                         GTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATAT    <  SRR4302541.7615196/94‑1 (MQ=60)
                                                                                         GTGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATAT    <  SRR4302541.745601/94‑1 (MQ=60)
                                                                                          TGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATC   >  SRR4302541.1556616/1‑94 (MQ=60)
                                                                                          TGGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATC   >  SRR4302541.978359/1‑94 (MQ=60)
                                                                                          TGGCTGAAGAACTATCATAGCCTGCAAGTGGCCTGAGAGCGAAGGGCTATCCGGCTAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATC   <  SRR4302541.5527008/94‑1 (MQ=60)
                                                                                           GGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCG  <  SRR4302541.8669455/94‑1 (MQ=60)
                                                                                           GGCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCG  <  SRR4302541.1957410/94‑1 (MQ=60)
                                                                                            |                                                                                            
GGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCG  >  NC_000913/1533806‑1533990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑