Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,538,045 G→A P857L (CCG→CTG)  narZ ← cryptic nitrate reductase 2 alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,538,0450GA100.0% 830.6 / NA 213P857L (CCG→CTG) narZcryptic nitrate reductase 2 alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (116/97);  total (116/97)

Only 100 of 216 total aligned reads displayed.

GCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT  >  NC_000913/1537955‑1538134
                                                                                          |                                                                                         
gCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAga                                                                                         >  1:2812726/1‑93 (MQ=255)
gCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAga                                                                                         >  1:6010249/1‑93 (MQ=255)
gCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAga                                                                                         >  2:2032670/1‑93 (MQ=255)
  cacaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAgaga                                                                                       >  2:594080/1‑93 (MQ=255)
  cacaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAgaga                                                                                       <  2:5074569/93‑1 (MQ=255)
   acaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCagagag                                                                                      <  1:6053795/93‑1 (MQ=255)
   acaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCagagag                                                                                      <  2:1924989/93‑1 (MQ=255)
      atatTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCa                                                                                   >  2:1173489/1‑93 (MQ=255)
      atatTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCa                                                                                   >  2:1539968/1‑93 (MQ=255)
       tatTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCAt                                                                                  <  2:840021/93‑1 (MQ=255)
       tatTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCAt                                                                                  >  2:6438061/1‑93 (MQ=255)
           cGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAg                                                                              <  2:6193111/93‑1 (MQ=255)
           cGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAg                                                                              <  1:2533051/93‑1 (MQ=255)
            ggCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGt                                                                             <  1:5518920/93‑1 (MQ=255)
             gcgcGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTa                                                                            <  2:5712404/93‑1 (MQ=255)
             gcgcGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTa                                                                            <  1:1627322/93‑1 (MQ=255)
              cgcgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAg                                                                           >  2:5255113/1‑93 (MQ=255)
               gcgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAgg                                                                          >  2:1081240/1‑93 (MQ=255)
               gcgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAgg                                                                          >  1:331391/1‑93 (MQ=255)
               gcgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAgg                                                                          <  1:1024478/93‑1 (MQ=255)
                cgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAggg                                                                         >  1:1556767/1‑93 (MQ=255)
                cgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAggg                                                                         <  1:829903/93‑1 (MQ=255)
                cgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAggg                                                                         <  1:2059940/93‑1 (MQ=255)
                cgGTGTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAggg                                                                         <  1:2182904/93‑1 (MQ=255)
                 ggTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAgggg                                                                        <  1:5121536/93‑1 (MQ=255)
                       gCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGATGTaa                                                                  >  1:2943567/1‑93 (MQ=255)
                        ccTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCTTGCAGTAGGGGTTGTAAt                                                                 <  1:4078111/93‑1 (MQ=255)
                        ccTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAAt                                                                 <  1:1776714/93‑1 (MQ=255)
                         cTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAAtc                                                                >  2:4777538/1‑92 (MQ=255)
                         cTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATa                                                                >  2:4901202/1‑93 (MQ=255)
                         cTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATa                                                                >  1:5026015/1‑93 (MQ=255)
                         cTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATa                                                                <  1:2383567/93‑1 (MQ=255)
                         cTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATa                                                                <  2:1949510/93‑1 (MQ=255)
                         cTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATa                                                                >  1:35655/1‑93 (MQ=255)
                         cTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATa                                                                <  2:4587955/93‑1 (MQ=255)
                           ggAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATAcc                                                              >  2:2903183/1‑93 (MQ=255)
                            gAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCa                                                             <  2:134072/93‑1 (MQ=255)
                            gAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCa                                                             >  1:6360116/1‑93 (MQ=255)
                             aaTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCAc                                                            <  2:4130612/93‑1 (MQ=255)
                             aaTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCAc                                                            >  2:4505186/1‑93 (MQ=255)
                              aTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACg                                                           >  2:1402346/1‑93 (MQ=255)
                              aTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACg                                                           >  1:3139346/1‑93 (MQ=255)
                               tCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGt                                                          >  2:4283035/1‑93 (MQ=255)
                                cAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTc                                                         <  1:1175899/93‑1 (MQ=255)
                                  gATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCgg                                                       >  1:3629198/1‑93 (MQ=255)
                                  gATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCgg                                                       <  2:639410/93‑1 (MQ=255)
                                   aTCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGt                                                      <  1:2371245/93‑1 (MQ=255)
                                    tCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGtt                                                     <  1:411812/93‑1 (MQ=255)
                                     cGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGttt                                                    >  2:2478177/1‑93 (MQ=255)
                                      gCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTc                                                   <  2:6112094/93‑1 (MQ=255)
                                      gCATTCGCCTATGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTc                                                   >  1:3929209/1‑93 (MQ=255)
                                         ttCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGGTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCttt                                                >  2:3443940/1‑93 (MQ=255)
                                         ttCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCttt                                                >  2:4376668/1‑93 (MQ=255)
                                           cGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGc                                              >  1:4886805/1‑93 (MQ=255)
                                           cGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGc                                              <  2:4603565/93‑1 (MQ=255)
                                            gCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCCCTGCCAGAGAGTCATGCAGTAGGGGGTGTAATACCACGTCGGTTTCTTTGcc                                             >  1:1636411/1‑93 (MQ=255)
                                            cccTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGcc                                             <  1:6183097/92‑1 (MQ=255)
                                              cTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCaa                                           >  1:3743304/1‑93 (MQ=255)
                                               tttGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAg                                          <  2:3756308/93‑1 (MQ=255)
                                               tttGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAg                                          >  1:4563622/1‑93 (MQ=255)
                                                 tGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAt                                        >  1:4449704/1‑93 (MQ=255)
                                                 tGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAt                                        <  2:5179229/93‑1 (MQ=255)
                                                 tGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAt                                        >  1:5991630/1‑93 (MQ=255)
                                                  gcgcCAGTCGAGCACTTCACACGTCTGTGAGAGCTCCGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATg                                       <  1:4276637/93‑1 (MQ=255)
                                                    gcCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAc                                     >  1:569231/1‑93 (MQ=255)
                                                     ccAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAcc                                    >  2:4934632/1‑93 (MQ=255)
                                                     ccAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAcc                                    >  1:1423285/1‑93 (MQ=255)
                                                     ccAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAcc                                    >  1:4422822/1‑93 (MQ=255)
                                                     ccAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAcc                                    <  1:1683178/93‑1 (MQ=255)
                                                     ccAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAcc                                    >  1:5976147/1‑93 (MQ=255)
                                                        gTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCAc                                 >  1:2200608/1‑93 (MQ=255)
                                                         tCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACg                                <  1:1902095/93‑1 (MQ=255)
                                                         tCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACg                                <  2:3565916/93‑1 (MQ=255)
                                                          cGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGc                               <  1:4400141/93‑1 (MQ=255)
                                                          cGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATACAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGc                               >  1:2798458/1‑93 (MQ=255)
                                                           gAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGca                              >  1:1927343/1‑93 (MQ=255)
                                                           gAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGca                              >  1:3068378/1‑93 (MQ=255)
                                                            aGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGcac                             >  2:2582643/1‑93 (MQ=255)
                                                              cACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCacac                           <  1:3423462/93‑1 (MQ=255)
                                                               aCTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACt                          <  2:3412443/93‑1 (MQ=255)
                                                               aCTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACt                          <  2:3849105/93‑1 (MQ=255)
                                                                cTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACtt                         >  2:1237558/1‑93 (MQ=255)
                                                                 ttCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTg                        >  2:5385534/1‑93 (MQ=255)
                                                                  tCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGc                       <  1:593771/93‑1 (MQ=255)
                                                                    acacGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGa                     >  2:2718401/1‑93 (MQ=255)
                                                                        ggCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAAt                 >  1:5162944/1‑93 (MQ=255)
                                                                        ggCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAAt                 >  2:3140610/1‑93 (MQ=255)
                                                                         gCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATg                <  1:3382376/93‑1 (MQ=255)
                                                                          cTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGc               <  2:2531440/93‑1 (MQ=255)
                                                                             tgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCttt            >  2:3260012/1‑93 (MQ=255)
                                                                             tgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCttt            <  1:533236/93‑1 (MQ=255)
                                                                              gagaGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTg           <  1:2476602/93‑1 (MQ=255)
                                                                               agagCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTgg          <  2:475996/93‑1 (MQ=255)
                                                                                gagCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGc         <  1:4717673/93‑1 (MQ=255)
                                                                                     ctGCCAGAGAGTCATGCAGTAGGGTTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATAc    <  1:4953083/93‑1 (MQ=255)
                                                                                     ctGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATAc    <  2:2619663/93‑1 (MQ=255)
                                                                                     ctGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATAc    <  2:1781867/93‑1 (MQ=255)
                                                                                     ctGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATAc    >  2:3765372/1‑93 (MQ=255)
                                                                                      tGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATAcc   >  1:6268947/1‑93 (MQ=255)
                                                                                       gCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCt  >  2:5153545/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT  >  NC_000913/1537955‑1538134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGT  >  NC_000913/1537953‑1538138
                                                                                            |                                                                                             
CCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTTAGAGCTCTGCCAG                                                                                              <  SRR4302534.4413399/94‑1 (MQ=60)
CCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAG                                                                                              <  SRR4302534.5832053/94‑1 (MQ=60)
 CGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGA                                                                                             >  SRR4302534.2886668/1‑94 (MQ=60)
 CGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGA                                                                                             >  SRR4302534.6173548/1‑94 (MQ=60)
  GCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAG                                                                                            <  SRR4302534.2487535/94‑1 (MQ=60)
     ACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGT                                                                                         <  SRR4302534.6218417/94‑1 (MQ=60)
      CAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTC                                                                                        <  SRR4302534.79805/94‑1 (MQ=60)
      CAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTC                                                                                        >  SRR4302534.4530618/1‑94 (MQ=60)
       AATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCA                                                                                       <  SRR4302534.5726268/94‑1 (MQ=60)
         TATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATG                                                                                     <  SRR4302534.6101879/94‑1 (MQ=60)
            TCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAG                                                                                  <  SRR4302534.2725948/94‑1 (MQ=60)
             CGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGT                                                                                 <  SRR4302534.1967767/94‑1 (MQ=60)
             CGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGT                                                                                 <  SRR4302534.2600256/94‑1 (MQ=60)
              GGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTA                                                                                <  SRR4302534.5667834/94‑1 (MQ=60)
               GCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCgagcacaag                                                                               >  SRR4302534.5964780/1‑85 (MQ=60)
               GCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAG                                                                               <  SRR4302534.1669779/94‑1 (MQ=60)
               GCGCGGTTTTGCCTGAAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAG                                                                               <  SRR4302534.2435139/94‑1 (MQ=60)
                CGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGG                                                                              >  SRR4302534.339909/1‑94 (MQ=60)
                 GCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGG                                                                             >  SRR4302534.1597522/1‑94 (MQ=60)
                 GCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGG                                                                             <  SRR4302534.1050860/94‑1 (MQ=60)
                  CGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGG                                                                            <  SRR4302534.850782/94‑1 (MQ=60)
                  CGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGG                                                                            <  SRR4302534.2113789/94‑1 (MQ=60)
                  CGGTGTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGG                                                                            <  SRR4302534.2240610/94‑1 (MQ=60)
                   GGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGT                                                                           <  SRR4302534.5259825/94‑1 (MQ=60)
                    GTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTT                                                                          <  SRR4302534.2208440/94‑1 (MQ=60)
                      TTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGT                                                                        <  SRR4302534.4879857/94‑1 (MQ=60)
                       TTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTA                                                                       >  SRR4302534.175367/1‑94 (MQ=60)
                       TTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTA                                                                       >  SRR4302534.6261388/1‑94 (MQ=60)
                        TGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGATGTAA                                                                      >  SRR4302534.3020804/1‑94 (MQ=60)
                        TGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGCGGTTGTAA                                                                      <  SRR4302534.6530685/94‑1 (MQ=60)
                         GCCTGGAAGCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAAT                                                                     >  SRR4302534.6298246/1‑94 (MQ=60)
                          CCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCTTGCAGTAGGGGTTGTAATA                                                                    <  SRR4302534.4187558/94‑1 (MQ=60)
                          CCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATA                                                                    >  SRR4302534.5161769/1‑94 (MQ=60)
                          CCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATA                                                                    <  SRR4302534.1822855/94‑1 (MQ=60)
                          CCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATA                                                                    >  SRR4302534.36598/1‑94 (MQ=60)
                           CTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATAC                                                                   <  SRR4302534.2446904/94‑1 (MQ=60)
                            TGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACC                                                                  >  SRR4302534.1478687/1‑94 (MQ=60)
                             GGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCA                                                                 >  SRR4302534.1599373/1‑94 (MQ=60)
                             GGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCA                                                                 >  SRR4302534.6531875/1‑94 (MQ=60)
                               AATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACG                                                               >  SRR4302534.5702020/1‑94 (MQ=60)
                               AATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACG                                                               >  SRR4302534.3966925/1‑94 (MQ=60)
                               AATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACG                                                               >  SRR4302534.3221909/1‑94 (MQ=60)
                               AATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACG                                                               >  SRR4302534.1071620/1‑94 (MQ=60)
                                 TCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTC                                                             >  SRR4302534.143871/1‑94 (MQ=60)
                                  CAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCG                                                            <  SRR4302534.1206806/94‑1 (MQ=60)
                                  CAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCG                                                            <  SRR4302534.5237124/94‑1 (MQ=60)
                                   AGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGG                                                           >  SRR4302534.3726757/1‑94 (MQ=60)
                                    GATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGT                                                          <  SRR4302534.2255614/94‑1 (MQ=60)
                                     ATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTT                                                         <  SRR4302534.2434197/94‑1 (MQ=60)
                                      TCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTT                                                        <  SRR4302534.422339/94‑1 (MQ=60)
                                       CGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTC                                                       >  SRR4302534.4607958/1‑94 (MQ=60)
                                       CGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTC                                                       <  SRR4302534.2571356/94‑1 (MQ=60)
                                       CGCATTCGCCTATGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTC                                                       >  SRR4302534.4034464/1‑94 (MQ=60)
                                         CATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTT                                                     >  SRR4302534.2882164/1‑94 (MQ=60)
                                         CATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTT                                                     <  SRR4302534.6067582/94‑1 (MQ=60)
                                           TTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTG                                                   <  SRR4302534.3211729/94‑1 (MQ=60)
                                            TCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGC                                                  >  SRR4302534.5019325/1‑94 (MQ=60)
                                            TCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGC                                                  >  SRR4302534.3351847/1‑94 (MQ=60)
                                             CGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCCCTGCCAGAGAGTCATGCAGTAGGGGGTGTAATACCACGTCGGTTTCTTTGCC                                                 >  SRR4302534.1679065/1‑94 (MQ=60)
                                              CCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCA                                                <  SRR4302534.6351390/94‑1 (MQ=60)
                                               CCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGacag                                               >  SRR4302534.6200313/1‑90 (MQ=60)
                                               CCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAA                                               >  SRR4302534.3800832/1‑94 (MQ=60)
                                               CCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAA                                               >  SRR4302534.3843873/1‑94 (MQ=60)
                                                CTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAG                                              >  SRR4302534.4687350/1‑94 (MQ=60)
                                                  TTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAT                                            >  SRR4302534.4569960/1‑94 (MQ=60)
                                                  TTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAT                                            <  SRR4302534.167575/94‑1 (MQ=60)
                                                  TTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAT                                            >  SRR4302534.6154400/1‑94 (MQ=60)
                                                    GCGCCAGTCGAGCACTTCACACGTCTGTGAGAGCTCCGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGA                                          <  SRR4302534.4392337/94‑1 (MQ=60)
                                                    GCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGA                                          >  SRR4302534.1757440/1‑94 (MQ=60)
                                                     CGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAC                                         >  SRR4302534.583202/1‑94 (MQ=60)
                                                      GCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACC                                        >  SRR4302534.4542234/1‑94 (MQ=60)
                                                      GCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACC                                        >  SRR4302534.6138462/1‑94 (MQ=60)
                                                      GCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACC                                        >  SRR4302534.1460728/1‑94 (MQ=60)
                                                       CCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCC                                       <  SRR4302534.1726957/94‑1 (MQ=60)
                                                       CCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCC                                       <  SRR4302534.4499363/94‑1 (MQ=60)
                                                        CAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGATTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCA                                      <  SRR4302534.5542912/94‑1 (MQ=60)
                                                         AGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCAC                                     >  SRR4302534.2258841/1‑94 (MQ=60)
                                                         AGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCAC                                     >  SRR4302534.6171987/1‑94 (MQ=60)
                                                           TCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGC                                   <  SRR4302534.2085318/94‑1 (MQ=60)
                                                           TCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGC                                   <  SRR4302534.1951705/94‑1 (MQ=60)
                                                           TCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATACAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGC                                   >  SRR4302534.2872054/1‑94 (MQ=60)
                                                            CGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCA                                  >  SRR4302534.1977732/1‑94 (MQ=60)
                                                            CGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCA                                  >  SRR4302534.3148965/1‑94 (MQ=60)
                                                            CGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCA                                  >  SRR4302534.3718879/1‑94 (MQ=60)
                                                            CGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCA                                  <  SRR4302534.4518860/94‑1 (MQ=60)
                                                                CACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACT                              <  SRR4302534.3515447/94‑1 (MQ=60)
                                                                 ACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTT                             <  SRR4302534.5109866/94‑1 (MQ=60)
                                                                  CTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTG                            >  SRR4302534.4046908/1‑94 (MQ=60)
                                                                   TTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGC                           <  SRR4302534.2404631/94‑1 (MQ=60)
                                                                    TCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCG                          >  SRR4302534.4220797/1‑94 (MQ=60)
                                                                    TCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCG                          <  SRR4302534.608263/94‑1 (MQ=60)
                                                                     CACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGA                         >  SRR4302534.1907054/1‑94 (MQ=60)
                                                                      ACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAA                        >  SRR4302534.2999735/1‑94 (MQ=60)
                                                                        ACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAA                      >  SRR4302534.4391641/1‑94 (MQ=60)
                                                                         CGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAAT                     >  SRR4302534.5302130/1‑94 (MQ=60)
                                                                         CGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGGTGTAATACCACGTCGGTTGCTGTGTGAAGATGACCCACGCACACTTGCGAgaag                     >  SRR4302534.4375374/1‑90 (MQ=60)
                                                                          GGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATG                    <  SRR4302534.6143363/94‑1 (MQ=60)
                                                                          GGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATG                    <  SRR4302534.5071407/94‑1 (MQ=60)
                                                                           GCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGC                   <  SRR4302534.3473050/94‑1 (MQ=60)
                                                                           GCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGC                   <  SRR4302534.503794/94‑1 (MQ=60)
                                                                             TGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTT                 >  SRR4302534.1704682/1‑94 (MQ=60)
                                                                              GTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTT                <  SRR4302534.5265595/94‑1 (MQ=60)
                                                                               TGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTG               <  SRR4302534.546476/94‑1 (MQ=60)
                                                                                GAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGG              <  SRR4302534.4557800/94‑1 (MQ=60)
                                                                                GAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGG              <  SRR4302534.2542412/94‑1 (MQ=60)
                                                                                  GAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCG            <  SRR4302534.3591003/94‑1 (MQ=60)
                                                                                  GAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCG            <  SRR4302534.4845785/94‑1 (MQ=60)
                                                                                       CTGCCAGAGAGTCATGCAGTAGGGTTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACC       <  SRR4302534.5087235/94‑1 (MQ=60)
                                                                                       CTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACC       <  SRR4302534.2343493/94‑1 (MQ=60)
                                                                                       CTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACC       >  SRR4302534.6438695/1‑94 (MQ=60)
                                                                                        TGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT      >  SRR4302534.3543966/1‑94 (MQ=60)
                                                                                        TGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT      <  SRR4302534.6400160/94‑1 (MQ=60)
                                                                                        TGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT      <  SRR4302534.3025759/94‑1 (MQ=60)
                                                                                        TGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT      <  SRR4302534.3505499/94‑1 (MQ=60)
                                                                                        TGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT      >  SRR4302534.4400750/1‑94 (MQ=60)
                                                                                        TGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCAGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT      >  SRR4302534.4813387/1‑94 (MQ=60)
                                                                                         GCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTT     <  SRR4302534.5431808/94‑1 (MQ=60)
                                                                                         GCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTT     <  SRR4302534.3441246/94‑1 (MQ=60)
                                                                                          CCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGTTTTGGCGATCCCTTT    >  SRR4302534.2825408/1‑94 (MQ=60)
                                                                                           CAGAGAGTCATGCAGTGGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG   <  SRR4302534.6056022/94‑1 (MQ=60)
                                                                                           CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG   <  SRR4302534.608575/94‑1 (MQ=60)
                                                                                           CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG   >  SRR4302534.6025008/1‑94 (MQ=60)
                                                                                           CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG   <  SRR4302534.2844362/94‑1 (MQ=60)
                                                                                           CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG   >  SRR4302534.1300675/1‑94 (MQ=60)
                                                                                            AGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGT  >  SRR4302534.4290105/1‑94 (MQ=60)
                                                                                            AGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGT  <  SRR4302534.3496333/94‑1 (MQ=60)
                                                                                            AGAGAGTCATGCAGTAGGGGGTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGT  >  SRR4302534.6405445/1‑94 (MQ=60)
                                                                                            |                                                                                             
CCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGT  >  NC_000913/1537953‑1538138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑