Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,538,045 G→A P857L (CCG→CTG)  narZ ← cryptic nitrate reductase 2 alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,538,0450GA99.4% 1101.0 / ‑3.7 310P857L (CCG→CTG) narZcryptic nitrate reductase 2 alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (154/154);  total (155/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 324 total aligned reads displayed.

GCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT  >  NC_000913/1537955‑1538134
                                                                                          |                                                                                         
gCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAga                                                                                         <  1:4579892/93‑1 (MQ=255)
 ccACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCagag                                                                                        >  2:615001/1‑93 (MQ=255)
  cacaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAgaga                                                                                       >  1:8158402/1‑93 (MQ=255)
  cacaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAgaga                                                                                       >  2:1246914/1‑93 (MQ=255)
   acaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCagagag                                                                                      >  2:1128109/1‑93 (MQ=255)
      atatTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCa                                                                                   >  2:2101612/1‑93 (MQ=255)
       tatTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGTGCACTTCACACGGCTGTGAGGGATTTGCCAGAGAGTAAt                                                                                  >  2:4484178/1‑93 (MQ=255)
       tatTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCAt                                                                                  >  1:3894122/1‑93 (MQ=255)
         ttCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGc                                                                                <  1:22789/93‑1 (MQ=255)
            tgcgcGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGCGTCATGCAGt                                                                             >  2:4291163/2‑93 (MQ=255)
              cgcgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAg                                                                           >  1:8678812/1‑93 (MQ=255)
              cgcgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAg                                                                           <  2:5054014/93‑1 (MQ=255)
              cgcgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAg                                                                           <  1:5860390/93‑1 (MQ=255)
               gcgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAgg                                                                          <  2:4084977/93‑1 (MQ=255)
               gcgGGTTTGCCTGGAATCAGATCCCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAgg                                                                          <  1:5262459/93‑1 (MQ=255)
                cgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAggg                                                                         >  2:3215224/1‑93 (MQ=255)
                cgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAggg                                                                         >  2:8564213/1‑93 (MQ=255)
                  gTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGt                                                                       >  1:6039622/1‑93 (MQ=255)
                  gTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGt                                                                       <  1:8234778/93‑1 (MQ=255)
                       gCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTaa                                                                  <  1:4368788/93‑1 (MQ=255)
                         cTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATa                                                                >  1:1003206/1‑93 (MQ=255)
                          tGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATAc                                                               >  1:4051211/1‑93 (MQ=255)
                          tGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATAc                                                               >  1:7379449/1‑93 (MQ=255)
                          tGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGACATGCAGTAGGGGTTGTAATAc                                                               >  1:3564564/1‑93 (MQ=255)
                            gAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCa                                                             >  1:2029527/1‑93 (MQ=255)
                            gAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCa                                                             >  2:2359554/1‑93 (MQ=255)
                            gAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGGTGTAATACCa                                                             >  1:5937344/1‑93 (MQ=255)
                             aaTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCAc                                                            <  2:1903096/93‑1 (MQ=255)
                             aaTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCAc                                                            <  1:1489720/93‑1 (MQ=255)
                             aaTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCAc                                                            <  2:2999757/93‑1 (MQ=255)
                             aaTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCAc                                                            <  2:4029597/93‑1 (MQ=255)
                              aTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACg                                                           >  1:5112767/1‑93 (MQ=255)
                              aTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACg                                                           <  2:7425058/93‑1 (MQ=255)
                               tCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGt                                                          <  2:1802343/93‑1 (MQ=255)
                                cAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGTTTGTAATACCACgcg                                                         >  1:1224898/1‑91 (MQ=255)
                                cAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGAGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAGTACCACGTc                                                         <  2:2723956/93‑1 (MQ=255)
                                 aGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCg                                                        >  2:8435210/1‑93 (MQ=255)
                                  gATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCgg                                                       >  1:2555555/1‑93 (MQ=255)
                                  gATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCgg                                                       <  2:7114404/93‑1 (MQ=255)
                                  gATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCgg                                                       >  2:1548458/1‑93 (MQ=255)
                                   aTCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGt                                                      >  1:311554/1‑93 (MQ=255)
                                   aTCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGt                                                      >  2:6914237/1‑93 (MQ=255)
                                    tCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGtt                                                     <  2:5333493/93‑1 (MQ=255)
                                    tCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGtt                                                     <  2:1488296/93‑1 (MQ=255)
                                      gCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTc                                                   >  2:6265456/1‑93 (MQ=255)
                                         ttCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCttt                                                <  1:6893810/93‑1 (MQ=255)
                                         ttCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCttt                                                <  2:6853383/93‑1 (MQ=255)
                                         ttCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCttt                                                <  1:7705484/93‑1 (MQ=255)
                                         ttCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTCTCttt                                                >  1:4784400/1‑93 (MQ=255)
                                            gCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGcc                                             >  1:5341951/1‑93 (MQ=255)
                                            gCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGcc                                             <  2:5421568/93‑1 (MQ=255)
                                            gCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGcc                                             <  1:1652869/93‑1 (MQ=255)
                                            cccTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGcc                                             <  2:4227199/92‑1 (MQ=255)
                                             ccTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCa                                            >  2:5569941/1‑93 (MQ=255)
                                             ccTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCa                                            >  1:7481100/1‑93 (MQ=255)
                                             ccTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCa                                            >  2:773472/1‑93 (MQ=255)
                                              cTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCaa                                           >  1:5695316/1‑93 (MQ=255)
                                               tttGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTCCCAAg                                          >  1:1848810/1‑93 (MQ=255)
                                                ttGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGa                                         >  1:1113865/1‑93 (MQ=255)
                                                ttGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGa                                         <  2:2492997/93‑1 (MQ=255)
                                                   cgcCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACTACGTCGGTTTCTTTGCCAAGATgc                                      >  1:4948946/1‑92 (MQ=255)
                                                   cgcCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGa                                      <  1:2163143/93‑1 (MQ=255)
                                                     ccAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAcc                                    <  2:7924561/93‑1 (MQ=255)
                                                     ccAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGAAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAcc                                    >  1:3072171/1‑93 (MQ=255)
                                                         tCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACg                                <  2:8405132/93‑1 (MQ=255)
                                                         tCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACg                                <  2:5094053/93‑1 (MQ=255)
                                                         tCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATAACACGTCGGTTTCTTTGCCAAGATGACCCACg                                >  2:7958509/1‑93 (MQ=255)
                                                            aGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGcac                             >  2:16986/1‑93 (MQ=255)
                                                                cTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGCCCCACGCACACtt                         >  2:751772/1‑93 (MQ=255)
                                                                cTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACtt                         >  1:3318258/1‑93 (MQ=255)
                                                                cTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACtt                         >  1:7521922/1‑93 (MQ=255)
                                                                 ttCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTg                        <  2:4808861/93‑1 (MQ=255)
                                                                   cacaCGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCg                      <  2:6681612/93‑1 (MQ=255)
                                                                     cacGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGaa                    >  2:8645832/1‑93 (MQ=255)
                                                                     cacGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGaa                    <  2:100160/93‑1 (MQ=255)
                                                                       cGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGaaaa                  >  2:8200416/1‑93 (MQ=255)
                                                                       cGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGaaaa                  >  1:1295171/1‑93 (MQ=255)
                                                                        ggCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAAt                 <  2:344741/93‑1 (MQ=255)
                                                                        ggCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAAt                 <  2:8114096/93‑1 (MQ=255)
                                                                           tgtgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCt              <  1:709223/93‑1 (MQ=255)
                                                                           tgtgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCt              >  2:3697047/1‑93 (MQ=255)
                                                                            gtgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGcct             >  2:4682970/1‑91 (MQ=255)
                                                                            gtgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCtt             <  1:7201163/93‑1 (MQ=255)
                                                                             tgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCttt            <  1:6223400/93‑1 (MQ=255)
                                                                             tgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCttt            <  2:5801173/93‑1 (MQ=255)
                                                                             tgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCttt            <  1:6721597/93‑1 (MQ=255)
                                                                             tgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCttt            <  2:245952/93‑1 (MQ=255)
                                                                             tgAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCttt            <  2:7688533/93‑1 (MQ=255)
                                                                              gagaGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTg           <  1:6532423/93‑1 (MQ=255)
                                                                              gagaGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTg           <  1:2685439/93‑1 (MQ=255)
                                                                               agagCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTgg          <  2:2908694/93‑1 (MQ=255)
                                                                                gagCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGc         >  2:3814935/1‑93 (MQ=255)
                                                                                gagCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGc         >  1:4449858/1‑93 (MQ=255)
                                                                                 agCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCg        >  2:389551/1‑93 (MQ=255)
                                                                                 agCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCg        >  2:2590740/1‑93 (MQ=255)
                                                                                  gCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGa       <  1:1355347/93‑1 (MQ=255)
                                                                                   ctctGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGAt      >  1:3414272/1‑93 (MQ=255)
                                                                                    tctGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATa     >  1:3820051/1‑93 (MQ=255)
                                                                                     ctGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATAc    <  1:5489951/93‑1 (MQ=255)
                                                                                       gCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCt  <  2:3653526/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT  >  NC_000913/1537955‑1538134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  >  NC_000913/1537952‑1538137
                                                                                             |                                                                                            
CCCGCCCCAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCCCTTCACACGGCTGTGAGAGCTCTGCCA                                                                                              <  SRR4302541.8457321/94‑1 (MQ=60)
CCCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCA                                                                                              <  SRR4302541.4208511/94‑1 (MQ=60)
ACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCA                                                                                              >  SRR4302541.7934321/1‑94 (MQ=60)
  CGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGA                                                                                            >  SRR4302541.3110366/1‑94 (MQ=60)
  CGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGA                                                                                            >  SRR4302541.391494/1‑94 (MQ=60)
  CGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGA                                                                                            >  SRR4302541.5451104/1‑94 (MQ=60)
   GCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAG                                                                                           <  SRR4302541.4626292/94‑1 (MQ=60)
   GCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAG                                                                                           <  SRR4302541.7038773/94‑1 (MQ=60)
    CCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGA                                                                                          >  SRR4302541.8247553/1‑94 (MQ=60)
    CCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGA                                                                                          <  SRR4302541.5978408/94‑1 (MQ=60)
     CACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAG                                                                                         >  SRR4302541.5525511/1‑94 (MQ=60)
      ACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGT                                                                                        >  SRR4302541.6012557/1‑94 (MQ=60)
      ACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGT                                                                                        >  SRR4302541.5917748/1‑94 (MQ=60)
      ACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGT                                                                                        <  SRR4302541.6929677/94‑1 (MQ=60)
       CAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTC                                                                                       <  SRR4302541.6030359/94‑1 (MQ=60)
       CAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTC                                                                                       >  SRR4302541.8066353/1‑94 (MQ=60)
        AATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCA                                                                                      >  SRR4302541.6644484/1‑94 (MQ=60)
         ATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCAT                                                                                     >  SRR4302541.1148022/1‑94 (MQ=60)
         ATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCAT                                                                                     >  SRR4302541.3933131/1‑94 (MQ=60)
          TATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATG                                                                                    <  SRR4302541.491764/94‑1 (MQ=60)
           ATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGC                                                                                   >  SRR4302541.4677256/1‑94 (MQ=60)
           ATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGC                                                                                   <  SRR4302541.1725046/94‑1 (MQ=60)
           ATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGC                                                                                   <  SRR4302541.8408128/94‑1 (MQ=60)
           ATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGC                                                                                   <  SRR4302541.4247247/94‑1 (MQ=60)
            TTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCA                                                                                  <  SRR4302541.7217372/94‑1 (MQ=60)
            TTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCA                                                                                  <  SRR4302541.22974/94‑1 (MQ=60)
             TCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAG                                                                                 >  SRR4302541.2746310/1‑94 (MQ=60)
             TCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAG                                                                                 >  SRR4302541.4492940/1‑94 (MQ=60)
                GCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAG                                                                              >  SRR4302541.8771678/1‑94 (MQ=60)
                 CGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGG                                                                             <  SRR4302541.5919990/94‑1 (MQ=60)
                 CGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGCAGG                                                                             >  SRR4302541.6259395/1‑94 (MQ=60)
                  GCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGG                                                                            <  SRR4302541.5316691/94‑1 (MQ=60)
                  GCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGG                                                                            <  SRR4302541.3852562/94‑1 (MQ=60)
                  GCGGGTTTGCCTGGAATCAGATCCCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGG                                                                            <  SRR4302541.5316692/94‑1 (MQ=60)
                   CGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGG                                                                           <  SRR4302541.1296132/94‑1 (MQ=60)
                    GGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGT                                                                          >  SRR4302541.5911631/1‑94 (MQ=60)
                    GGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGT                                                                          >  SRR4302541.6101438/1‑94 (MQ=60)
                     GTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTT                                                                         <  SRR4302541.8324539/94‑1 (MQ=60)
                       TTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGT                                                                       <  SRR4302541.1382739/94‑1 (MQ=60)
                       TTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGT                                                                       <  SRR4302541.705853/94‑1 (MQ=60)
                         TGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAA                                                                     >  SRR4302541.7159111/1‑94 (MQ=60)
                          GCCTGTAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAAT                                                                    <  SRR4302541.4458988/94‑1 (MQ=60)
                          GCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAAT                                                                    <  SRR4302541.4412441/94‑1 (MQ=60)
                           CCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATA                                                                   >  SRR4302541.1012520/1‑94 (MQ=60)
                           CCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATA                                                                   >  SRR4302541.8498613/1‑94 (MQ=60)
                            CTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATAC                                                                  >  SRR4302541.4091188/1‑94 (MQ=60)
                            CTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATAC                                                                  >  SRR4302541.4091187/1‑94 (MQ=60)
                            CTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATAC                                                                  >  SRR4302541.7458330/1‑94 (MQ=60)
                            CTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGACATGCAGTAGGGGTTGTAATAC                                                                  >  SRR4302541.3600652/1‑94 (MQ=60)
                             TGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACC                                                                 >  SRR4302541.291299/1‑94 (MQ=60)
                              GGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCA                                                                >  SRR4302541.2049130/1‑94 (MQ=60)
                              GGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGGTGTAATACCA                                                                >  SRR4302541.5997834/1‑94 (MQ=60)
                               GAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGCAGGGGTTGTAATACCAC                                                               >  SRR4302541.6783838/1‑94 (MQ=60)
                                AATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACG                                                              >  SRR4302541.5165283/1‑94 (MQ=60)
                                AATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACG                                                              <  SRR4302541.1504399/94‑1 (MQ=60)
                                 ATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGT                                                             >  SRR4302541.5241139/1‑94 (MQ=60)
                                  TCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGTTTGTAATACCACGcg                                                            >  SRR4302541.1236906/1‑92 (MQ=60)
                                  TCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTC                                                            <  SRR4302541.4619479/94‑1 (MQ=60)
                                  TCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTC                                                            <  SRR4302541.2358469/94‑1 (MQ=60)
                                  TCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTC                                                            <  SRR4302541.7597657/94‑1 (MQ=60)
                                   CAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCG                                                           >  SRR4302541.2895891/1‑94 (MQ=60)
                                    AGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGG                                                          >  SRR4302541.2581179/1‑94 (MQ=60)
                                    AGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGG                                                          <  SRR4302541.342163/94‑1 (MQ=60)
                                    AGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGG                                                          >  SRR4302541.3489790/1‑94 (MQ=60)
                                    AGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGG                                                          <  SRR4302541.5379398/94‑1 (MQ=60)
                                     GATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGT                                                         >  SRR4302541.314317/1‑94 (MQ=60)
                                     GATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGT                                                         <  SRR4302541.3124897/94‑1 (MQ=60)
                                     GATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGT                                                         >  SRR4302541.6826138/1‑94 (MQ=60)
                                        CGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTC                                                      >  SRR4302541.592719/1‑94 (MQ=60)
                                         GCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCT                                                     <  SRR4302541.7511586/94‑1 (MQ=60)
                                         GCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCT                                                     <  SRR4302541.2550257/94‑1 (MQ=60)
                                         GCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCT                                                     >  SRR4302541.1618657/1‑94 (MQ=60)
                                         GCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCT                                                     <  SRR4302541.6247270/94‑1 (MQ=60)
                                          CATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTT                                                    >  SRR4302541.2175559/1‑94 (MQ=60)
                                           ATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTT                                                   <  SRR4302541.2219521/94‑1 (MQ=60)
                                           ATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTCTCTTT                                                   >  SRR4302541.4833228/1‑94 (MQ=60)
                                            TTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTG                                                  >  SRR4302541.2666105/1‑94 (MQ=60)
                                            TTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTG                                                  <  SRR4302541.7787432/94‑1 (MQ=60)
                                            TTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTG                                                  <  SRR4302541.6967376/94‑1 (MQ=60)
                                              CGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCC                                                >  SRR4302541.5396866/1‑94 (MQ=60)
                                              CGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCC                                                <  SRR4302541.7732580/94‑1 (MQ=60)
                                               GCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCA                                               >  SRR4302541.7561128/1‑94 (MQ=60)
                                               GCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCA                                               <  SRR4302541.1669192/94‑1 (MQ=60)
                                                CCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAA                                              >  SRR4302541.5753394/1‑94 (MQ=60)
                                                CCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAA                                              >  SRR4302541.5753395/1‑94 (MQ=60)
                                                CCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAA                                              >  SRR4302541.5048275/1‑94 (MQ=60)
                                                 CTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAG                                             >  SRR4302541.4333822/1‑94 (MQ=60)
                                                 CTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAG                                             >  SRR4302541.391250/1‑94 (MQ=60)
                                                 CTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTCCCAAG                                             >  SRR4302541.1866689/1‑94 (MQ=60)
                                                  TTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGA                                            >  SRR4302541.732712/1‑94 (MQ=60)
                                                  TTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGA                                            >  SRR4302541.1124328/1‑94 (MQ=60)
                                                   TTGCTCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAT                                           <  SRR4302541.7519056/94‑1 (MQ=60)
                                                   TTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAT                                           <  SRR4302541.6432156/94‑1 (MQ=60)
                                                   TTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGAT                                           <  SRR4302541.1410781/94‑1 (MQ=60)
                                                    TGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATG                                          <  SRR4302541.1982824/94‑1 (MQ=60)
                                                    TGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATG                                          <  SRR4302541.623115/94‑1 (MQ=60)
                                                    TGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATG                                          <  SRR4302541.4698547/94‑1 (MQ=60)
                                                     GCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACTACGTCGGTTTCTTTGCCAAGATGC                                         >  SRR4302541.4999524/1‑94 (MQ=60)
                                                     GCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGA                                         <  SRR4302541.5682034/94‑1 (MQ=60)
                                                      CGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAC                                        >  SRR4302541.1048106/1‑94 (MQ=60)
                                                      CGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAC                                        <  SRR4302541.2184056/94‑1 (MQ=60)
                                                       GCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACC                                       <  SRR4302541.8339599/94‑1 (MQ=60)
                                                       GCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACC                                       >  SRR4302541.7585132/1‑94 (MQ=60)
                                                       GCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGAAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACC                                       >  SRR4302541.3103332/1‑94 (MQ=60)
                                                        CCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCC                                      <  SRR4302541.7307687/94‑1 (MQ=60)
                                                        CCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCC                                      <  SRR4302541.7634648/94‑1 (MQ=60)
                                                        CCAGTCGAGCACTTCACACGGCGTTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCC                                      >  SRR4302541.1541338/1‑94 (MQ=60)
                                                         CAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCA                                     >  SRR4302541.2168273/1‑94 (MQ=60)
                                                         CAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCA                                     >  SRR4302541.1323844/1‑94 (MQ=60)
                                                          AGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCAC                                    >  SRR4302541.7562742/1‑94 (MQ=60)
                                                           gtcggggcCTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACG                                   <  SRR4302541.1933036/86‑1 (MQ=60)
                                                           GTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACG                                   >  SRR4302541.4587758/1‑94 (MQ=60)
                                                             CGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCA                                 >  SRR4302541.1179606/1‑94 (MQ=60)
                                                             CGAGCACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACACA                                 >  SRR4302541.3496904/1‑94 (MQ=60)
                                                                GCACTGCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACAC                              <  SRR4302541.2145247/94‑1 (MQ=60)
                                                                  ACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTT                            >  SRR4302541.7602298/1‑94 (MQ=60)
                                                                  ACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTT                            >  SRR4302541.1452508/1‑94 (MQ=60)
                                                                  ACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTT                            >  SRR4302541.3351546/1‑94 (MQ=60)
                                                                  ACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTT                            <  SRR4302541.127276/94‑1 (MQ=60)
                                                                  ACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTT                            <  SRR4302541.8291978/94‑1 (MQ=60)
                                                                  ACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTT                            <  SRR4302541.3149338/94‑1 (MQ=60)
                                                                  ACTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTT                            <  SRR4302541.5678152/94‑1 (MQ=60)
                                                                   CTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTG                           <  SRR4302541.7193636/94‑1 (MQ=60)
                                                                   CTTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTG                           >  SRR4302541.2278102/1‑94 (MQ=60)
                                                                    TTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGC                          <  SRR4302541.8276698/94‑1 (MQ=60)
                                                                    TTCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGC                          >  SRR4302541.5011222/1‑94 (MQ=60)
                                                                     TCACACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCG                         <  SRR4302541.485506/94‑1 (MQ=60)
                                                                        CACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAA                      >  SRR4302541.4943061/1‑94 (MQ=60)
                                                                         ACGGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAA                     >  SRR4302541.1307953/1‑94 (MQ=60)
                                                                           GGCTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATG                   <  SRR4302541.2703177/94‑1 (MQ=60)
                                                                             CTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATATCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCT                 >  SRR4302541.3986564/1‑94 (MQ=60)
                                                                             CTGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCT                 >  SRR4302541.1843266/1‑94 (MQ=60)
                                                                              TGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTT                <  SRR4302541.715896/94‑1 (MQ=60)
                                                                              TGTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTT                >  SRR4302541.3177095/1‑94 (MQ=60)
                                                                               GTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTT               <  SRR4302541.8185803/94‑1 (MQ=60)
                                                                               GTGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTT               <  SRR4302541.7277994/94‑1 (MQ=60)
                                                                                TGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTG              <  SRR4302541.6793327/94‑1 (MQ=60)
                                                                                TGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTG              <  SRR4302541.6286985/94‑1 (MQ=60)
                                                                                TGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTG              <  SRR4302541.6295300/94‑1 (MQ=60)
                                                                                 GAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGG             <  SRR4302541.6599111/94‑1 (MQ=60)
                                                                                 GAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGG             >  SRR4302541.1352947/1‑94 (MQ=60)
                                                                                 GAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGG             <  SRR4302541.2712117/94‑1 (MQ=60)
                                                                                 GAGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGG             >  SRR4302541.3420672/1‑94 (MQ=60)
                                                                                  AGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGC            >  SRR4302541.4197549/1‑94 (MQ=60)
                                                                                  AGAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGC            >  SRR4302541.4494611/1‑94 (MQ=60)
                                                                                   GAGCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGACAATGCTTTGGAG           >  SRR4302541.1106564/1‑94 (MQ=60)
                                                                                     GCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGAT         >  SRR4302541.3448356/1‑94 (MQ=60)
                                                                                     GCTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGAT         <  SRR4302541.1368738/94‑1 (MQ=60)
                                                                                      CTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATA        >  SRR4302541.8880905/1‑94 (MQ=60)
                                                                                      CTCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATA        >  SRR4302541.3858529/1‑94 (MQ=60)
                                                                                       TCTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATAC       >  SRR4302541.196401/1‑94 (MQ=60)
                                                                                        CTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACT      >  SRR4302541.5327937/1‑94 (MQ=60)
                                                                                        CTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACC      <  SRR4302541.5546210/94‑1 (MQ=60)
                                                                                        CTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACC      <  SRR4302541.3850366/94‑1 (MQ=60)
                                                                                        CTGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACC      <  SRR4302541.1008458/94‑1 (MQ=60)
                                                                                         TGCCAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCT     <  SRR4302541.3506799/94‑1 (MQ=60)
                                                                                            CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  >  SRR4302541.6473775/1‑94 (MQ=60)
                                                                                            CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  <  SRR4302541.5507940/94‑1 (MQ=60)
                                                                                            CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  >  SRR4302541.4120511/1‑94 (MQ=60)
                                                                                            CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  >  SRR4302541.2818083/1‑94 (MQ=60)
                                                                                            CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  <  SRR4302541.2865338/94‑1 (MQ=60)
                                                                                            CAGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  <  SRR4302541.3093992/94‑1 (MQ=60)
                                                                                            CAGAGAGTCATGCAGTAGGGGGTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  >  SRR4302541.6112597/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTG  >  NC_000913/1537952‑1538137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑