Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,592,156	(C)_8→7	coding (3955/3978 nt)	ydeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,592,149	0	C	.	99.5%	932.4 / ‑3.9	200	coding (3962/3978 nt)	ydeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (94/105); total (94/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

TACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGC  >  NC_000913/1592057‑1592237

 aCATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcc                                                                                         >  1:498499/1‑93 (MQ=255)

 aCATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcc                                                                                         >  1:4274629/1‑93 (MQ=255)

  cATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccc                                                                                        >  1:108880/1‑93 (MQ=255)

  cATTCCCCGGGTTCCCCCCTTGCCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccc                                                                                        <  1:4878090/93‑1 (MQ=255)

   aTTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcccc                                                                                       <  2:1310841/93‑1 (MQ=255)

    ttCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccccc                                                                                      >  1:1168390/1‑93 (MQ=255)

    ttCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccccc                                                                                      <  1:4658706/93‑1 (MQ=255)

    ttCCCCGGTTTACCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGCTGCCCCCGATAGAGGGTCTAGAGACAGTCAGTGacccc                                                                                      >  1:535805/1‑93 (MQ=255)

     tCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcccccc                                                                                     >  1:314073/1‑93 (MQ=255)

     tCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcccccc                                                                                     <  1:5300875/93‑1 (MQ=255)

     tCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcccccc                                                                                     <  1:2458326/93‑1 (MQ=255)

      ccccGGTTTCCCCCCTTCGCTATTCATTTGACCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccccccc                                                                                    <  2:2265975/93‑1 (MQ=255)

      ccccGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccccccc                                                                                    <  1:4831671/93‑1 (MQ=255)

       cccGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcccccccc                                                                                   >  2:1468153/1‑93 (MQ=255)

       cccGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccccccca                                                                                   <  2:4673151/93‑2 (MQ=255)

       cccGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGccccccca                                                                                   <  1:3612816/93‑2 (MQ=255)

        ccGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcccccccag                                                                                  >  2:3821357/1‑91 (MQ=255)

          ggTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGcccccccaggc                                                                                >  1:779622/1‑89 (MQ=255)

              tCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATaa                                                                           >  2:6163347/1‑93 (MQ=255)

                cccccTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACg                                                                         >  2:5336365/1‑93 (MQ=255)

                cccccTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGCCATAACg                                                                         >  1:5902218/1‑93 (MQ=255)

                 ccccTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGt                                                                        <  1:5676444/93‑1 (MQ=255)

                  cccTTCGCTCTTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGtt                                                                       <  1:5239614/93‑1 (MQ=255)

                  cccTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGtt                                                                       >  2:6308002/1‑93 (MQ=255)

                   ccTTCGCTATTCATTTGAAACGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTTGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTc                                                                      >  2:5602393/1‑93 (MQ=255)

                     ttCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCat                                                                    <  2:203097/93‑1 (MQ=255)

                      tCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCata                                                                   >  1:3041162/1‑93 (MQ=255)

                        gCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATc                                                                 >  1:3432633/1‑93 (MQ=255)

                         cTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCa                                                                >  1:6141609/1‑93 (MQ=255)

                         cTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCa                                                                >  1:517241/1‑93 (MQ=255)

                          tATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCAc                                                               >  2:1016089/1‑93 (MQ=255)

                           aTTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCAcc                                                              <  2:3613523/93‑1 (MQ=255)

                           aTTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCAcc                                                              <  2:1885457/93‑1 (MQ=255)

                           aTTCATTTAAACCGTGCCCCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCAcc                                                              <  2:2497780/93‑1 (MQ=255)

                              cATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGc                                                           <  2:897295/93‑1 (MQ=255)

                                tttGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAg                                                         >  1:4692576/1‑93 (MQ=255)

                                tttGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAg                                                         >  2:220656/1‑93 (MQ=255)

                                   gAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAgtgg                                                      <  1:748407/93‑1 (MQ=255)

                                    aaCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAgtggt                                                     >  1:1787684/1‑93 (MQ=255)

                                    aaCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAgtggt                                                     <  1:4126213/93‑1 (MQ=255)

                                     aCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAgtggtg                                                    >  2:6272393/1‑93 (MQ=255)

                                       cGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGAc                                                  <  2:3401130/93‑1 (MQ=255)

                                        gTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACa                                                 >  2:5448034/1‑93 (MQ=255)

                                        gTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACa                                                 >  1:1896665/1‑93 (MQ=255)

                                        gTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGGTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACa                                                 >  2:1195210/1‑93 (MQ=255)

                                         tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACAt                                                >  1:3971538/1‑93 (MQ=255)

                                          gccgccATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACAtt                                               <  1:6450429/93‑1 (MQ=255)

                                           cctccATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACAttt                                              <  2:4502142/90‑1 (MQ=255)

                                            cgccATTCTCCAGGTTGCCGCCGATAGTAGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTc                                             <  2:6466366/93‑1 (MQ=255)

                                               cATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCg                                          >  1:606171/1‑93 (MQ=255)

                                                aTTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGa                                         >  2:4855998/1‑93 (MQ=255)

                                                 ttCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGAc                                        >  1:1458615/1‑93 (MQ=255)

                                                  tctcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACa                                       >  2:1708716/1‑93 (MQ=255)

                                                   ctcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAt                                      >  1:661947/1‑93 (MQ=255)

                                                   ctcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAt                                      >  1:4146267/1‑93 (MQ=255)

                                                    tcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATc                                     <  1:5428272/93‑1 (MQ=255)

                                                    tcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATc                                     >  2:1125123/1‑93 (MQ=255)

                                                    tcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATc                                     <  1:1424970/93‑1 (MQ=255)

                                                    tcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATc                                     >  1:1520499/1‑93 (MQ=255)

                                                    tcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATc                                     <  1:2813976/93‑1 (MQ=255)

                                                      cAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATcac                                   >  1:1853525/1‑93 (MQ=255)

                                                       aGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATcaca                                  >  1:5630955/1‑93 (MQ=255)

                                                       aGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATcaca                                  <  2:1032515/93‑1 (MQ=255)

                                                       aGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATcaca                                  >  2:2556503/1‑93 (MQ=255)

                                                       aGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATcaca                                  <  1:5933628/93‑1 (MQ=255)

                                                        ggTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                 >  2:4832516/1‑93 (MQ=255)

                                                        ggTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                 <  1:3066290/93‑1 (MQ=255)

                                                        ggTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                 >  2:806422/1‑93 (MQ=255)

                                                        ggTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGGGACATTTCCCGACATCacac                                 >  1:2222459/1‑93 (MQ=255)

                                                          ttGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCg                               >  2:2286718/1‑93 (MQ=255)

                                                          ttGCCGCCGATAGTGGTTGTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTGCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCg                               <  1:5395497/93‑1 (MQ=255)

                                                           tGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGc                              <  2:1816904/93‑1 (MQ=255)

                                                            gccgccGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCt                             >  1:4445461/1‑93 (MQ=255)

                                                              cgccgATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGt                           <  2:2717664/93‑1 (MQ=255)

                                                               gccgATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGtt                          >  2:1518103/1‑93 (MQ=255)

                                                                 cgATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTAt                        <  1:3312906/93‑1 (MQ=255)

                                                                    tAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGa                     >  2:3001345/1‑93 (MQ=255)

                                                                     aGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGat                    >  1:5741567/1‑93 (MQ=255)

                                                                     aGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGat                    <  2:3050066/93‑1 (MQ=255)

                                                                      gTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGata                   <  1:5990532/93‑1 (MQ=255)

                                                                       tGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGatat                  <  2:5783405/93‑1 (MQ=255)

                                                                        ggTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATAtt                 <  1:5796380/93‑1 (MQ=255)

                                                                        ggTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATAtt                 <  2:1623365/93‑1 (MQ=255)

                                                                          ttttAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTaa               <  1:5085633/93‑1 (MQ=255)

                                                                          ttttAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTaa               >  1:319889/1‑93 (MQ=255)

                                                                          ttttAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTaa               <  1:4361084/93‑1 (MQ=255)

                                                                            ttAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAAtg             <  2:4149478/93‑1 (MQ=255)

                                                                              aGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAAtgtg           <  1:6220530/93‑1 (MQ=255)

                                                                               gCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATgtgt          >  2:2906166/1‑93 (MQ=255)

                                                                                cGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGtt         <  1:1914058/93‑1 (MQ=255)

                                                                                 gACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTg        >  2:4031115/1‑93 (MQ=255)

                                                                                 gACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTg        >  1:2049121/1‑93 (MQ=255)

                                                                                 gACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTg        <  1:3796470/93‑1 (MQ=255)

                                                                                  acacGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGa       <  1:2774461/93‑1 (MQ=255)

                                                                                   cacGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGAc      <  1:984118/93‑1 (MQ=255)

                                                                                    acGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACt     >  2:4608246/1‑93 (MQ=255)

                                                                                    acGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACt     <  1:134961/93‑1 (MQ=255)

                                                                                     cGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACtt    <  2:5886864/93‑1 (MQ=255)

                                                                                     cGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACtt    <  2:4254904/93‑1 (MQ=255)

                                                                                     cGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACtt    <  2:3457426/93‑1 (MQ=255)

TACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGC  >  NC_000913/1592057‑1592237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGG  >  NC_000913/1592063‑1592248

CCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCA                                                                                              >  SRR4302534.5329246/1‑94 (MQ=60)

CCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCA                                                                                              <  SRR4302534.4962753/94‑1 (MQ=60)

CCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCA                                                                                              <  SRR4302534.1978971/94‑1 (MQ=60)

  CCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGG                                                                                           <  SRR4302534.4787091/94‑1 (MQ=60)

  CCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGG                                                                                           <  SRR4302534.612235/94‑1 (MQ=60)

   CGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGC                                                                                          >  SRR4302534.799059/1‑94 (MQ=60)

    GGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACCCGCAGTG‑CCCCCCCAGGCA                                                                                         >  SRR4302534.4911660/1‑94 (MQ=60)

     GTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCAT                                                                                        <  SRR4302534.3208468/94‑1 (MQ=60)

        TCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTACCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAAC                                                                                     >  SRR4302534.4466014/1‑94 (MQ=60)

         CCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGCCATAACG                                                                                    >  SRR4302534.6062468/1‑94 (MQ=60)

           CCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTT                                                                                  <  SRR4302534.5830126/94‑1 (MQ=60)

           CCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTT                                                                                  <  SRR4302534.676793/94‑1 (MQ=60)

            CCCTTCGCTCTTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTC                                                                                 <  SRR4302534.5381089/94‑1 (MQ=60)

            CCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTC                                                                                 >  SRR4302534.188334/1‑94 (MQ=60)

             CCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCA                                                                                <  SRR4302534.5094918/94‑1 (MQ=60)

              CTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCAT                                                                               >  SRR4302534.811126/1‑94 (MQ=60)

              CTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCAT                                                                               <  SRR4302534.4730576/94‑1 (MQ=60)

               TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATA                                                                              >  SRR4302534.3120929/1‑94 (MQ=60)

               TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATA                                                                              <  SRR4302534.3887405/94‑1 (MQ=60)

                TCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATAT                                                                             <  SRR4302534.5443501/94‑1 (MQ=60)

                 CGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATC                                                                            >  SRR4302534.3524861/1‑94 (MQ=60)

                  GCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCA                                                                           >  SRR4302534.530096/1‑94 (MQ=60)

                  GCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCA                                                                           >  SRR4302534.6268764/1‑94 (MQ=60)

                  GCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCA                                                                           >  SRR4302534.6308653/1‑94 (MQ=60)

                  GCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGCTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCA                                                                           >  SRR4302534.6360773/1‑94 (MQ=60)

                    TATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACC                                                                         >  SRR4302534.5336454/1‑94 (MQ=60)

                     ATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCT                                                                        <  SRR4302534.1403246/94‑1 (MQ=60)

                        CATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCA                                                                     >  SRR4302534.3682568/1‑94 (MQ=60)

                         ATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAG                                                                    >  SRR4302534.4819954/1‑94 (MQ=60)

                          TTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGG                                                                   >  SRR4302534.2889021/1‑94 (MQ=60)

                          TTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGATTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGA                                                                   >  SRR4302534.5448537/1‑94 (MQ=60)

                           TTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTG                                                                  >  SRR4302534.63859/1‑94 (MQ=60)

                           TTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTG                                                                  <  SRR4302534.4724442/94‑1 (MQ=60)

                             GAGCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGT                                                                <  SRR4302534.2887117/94‑1 (MQ=60)

                             GAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGT                                                                <  SRR4302534.5907026/94‑1 (MQ=60)

                             GAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGT                                                                >  SRR4302534.5217231/1‑94 (MQ=60)

                             GAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGT                                                                >  SRR4302534.1834100/1‑94 (MQ=60)

                             GAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGT                                                                <  SRR4302534.766953/94‑1 (MQ=60)

                              AACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTG                                                               <  SRR4302534.4237311/94‑1 (MQ=60)

                              AACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTG                                                               <  SRR4302534.3358705/94‑1 (MQ=60)

                              AACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTG                                                               >  SRR4302534.6082066/1‑94 (MQ=60)

                              AACCGTGCCGCCATTCTCCAGGCTGCCGCCGATAGTGGTTTTAGCGACAGGCAGTG‑CCTCCCCAGGCATAACTTTCATAATACCTGCAGTGGTG                                                               >  SRR4302534.4192925/1‑94 (MQ=60)

                                CCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGAC                                                             >  SRR4302534.769134/1‑94 (MQ=60)

                                 CGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACA                                                            >  SRR4302534.1946128/1‑94 (MQ=60)

                                  GTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACAT                                                           >  SRR4302534.4077840/1‑94 (MQ=60)

                                   TGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATT                                                          <  SRR4302534.2385035/94‑1 (MQ=60)

                                    GCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTT                                                         <  SRR4302534.6624259/94‑1 (MQ=60)

                                      CGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCC                                                       >  SRR4302534.1414252/1‑94 (MQ=60)

                                       ccctTTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCC                                                      <  SRR4302534.3003831/90‑1 (MQ=60)

                                       GCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCG                                                      >  SRR4302534.4357282/1‑94 (MQ=60)

                                       GCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCC                                                      >  SRR4302534.3424901/1‑94 (MQ=60)

                                        CCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCG                                                     >  SRR4302534.620920/1‑94 (MQ=60)

                                         CATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGA                                                    >  SRR4302534.1305453/1‑94 (MQ=60)

                                          ATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGAC                                                   >  SRR4302534.1496978/1‑94 (MQ=60)

                                            TCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAT                                                 <  SRR4302534.1493093/94‑1 (MQ=60)

                                            TCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAT                                                 <  SRR4302534.6529483/94‑1 (MQ=60)

                                            TCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAT                                                 >  SRR4302534.678045/1‑94 (MQ=60)

                                            TCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAT                                                 >  SRR4302534.4258182/1‑94 (MQ=60)

                                            TCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAT                                                 >  SRR4302534.6219680/1‑94 (MQ=60)

                                             CTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATC                                                >  SRR4302534.1560427/1‑94 (MQ=60)

                                             CTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATC                                                >  SRR4302534.3990749/1‑94 (MQ=60)

                                              TCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCA                                               <  SRR4302534.5574391/94‑1 (MQ=60)

                                              TCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCA                                               <  SRR4302534.1462454/94‑1 (MQ=60)

                                              TCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCA                                               <  SRR4302534.2887953/94‑1 (MQ=60)

                                              TCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCA                                               >  SRR4302534.1513478/1‑94 (MQ=60)

                                               CCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCAC                                              <  SRR4302534.2418195/94‑1 (MQ=60)

                                               CCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCAC                                              >  SRR4302534.1901755/1‑94 (MQ=60)

                                                CAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACA                                             >  SRR4302534.5783357/1‑94 (MQ=60)

                                                CAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACA                                             >  SRR4302534.5753082/1‑94 (MQ=60)

                                                CAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACA                                             <  SRR4302534.1697717/94‑1 (MQ=60)

                                                 AGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACAC                                            <  SRR4302534.6094761/94‑1 (MQ=60)

                                                 AGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGGGACATTTCCCGACATCACAC                                            >  SRR4302534.2281321/1‑94 (MQ=60)

                                                  GTTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACCCC                                           <  SRR4302534.1949203/94‑1 (MQ=60)

                                                  GGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACC                                           <  SRR4302534.3146801/94‑1 (MQ=60)

                                                    TTGCCGCCGATAGTGGTTGTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTGCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGC                                         <  SRR4302534.5540767/94‑1 (MQ=60)

                                                     TGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCT                                        <  SRR4302534.1010818/94‑1 (MQ=60)

                                                     TGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCT                                        >  SRR4302534.5733571/1‑94 (MQ=60)

                                                     TGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCT                                        >  SRR4302534.4565571/1‑94 (MQ=60)

                                                     TGCCGCCGATAGTGGTTTTAGCGACACGCAGT‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACGCCGCT                                        <  SRR4302534.5525724/94‑1 (MQ=60)

                                                         CCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTA                                    <  SRR4302534.5177443/94‑1 (MQ=60)

                                                           CGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATG                                  >  SRR4302534.739876/1‑94 (MQ=60)

                                                           CGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATG                                  <  SRR4302534.3401409/94‑1 (MQ=60)

                                                            GATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGG                                 >  SRR4302534.2777595/1‑94 (MQ=60)

                                                            GATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGG                                 >  SRR4302534.3260265/1‑94 (MQ=60)

                                                             ATAGTGTTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGA                                <  SRR4302534.5799335/94‑1 (MQ=60)

                                                              TAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATGACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGAT                               >  SRR4302534.5235614/1‑94 (MQ=60)

                                                              TAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGAT                               <  SRR4302534.1987786/94‑1 (MQ=60)

                                                              TAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGAT                               >  SRR4302534.5897189/1‑94 (MQ=60)

                                                               AGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATA                              >  SRR4302534.918166/1‑94 (MQ=60)

                                                               AGTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATA                              >  SRR4302534.4461970/1‑94 (MQ=60)

                                                                GTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATAT                             <  SRR4302534.6153267/94‑1 (MQ=60)

                                                                GTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATAT                             >  SRR4302534.2168724/1‑94 (MQ=60)

                                                                GTGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATAT                             <  SRR4302534.4253148/94‑1 (MQ=60)

                                                                 TGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATT                            <  SRR4302534.3206377/94‑1 (MQ=60)

                                                                 TGGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATT                            <  SRR4302534.314073/94‑1 (MQ=60)

                                                                  GGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTA                           <  SRR4302534.1187599/94‑1 (MQ=60)

                                                                  GGTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTA                           <  SRR4302534.5953650/94‑1 (MQ=60)

                                                                   GTTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAA                          >  SRR4302534.328132/1‑94 (MQ=60)

                                                                    TTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAAT                         <  SRR4302534.5222900/94‑1 (MQ=60)

                                                                    TTTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAAT                         <  SRR4302534.4478721/94‑1 (MQ=60)

                                                                     TTTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATG                        <  SRR4302534.5540886/94‑1 (MQ=60)

                                                                      TTAGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGT                       <  SRR4302534.5837093/94‑1 (MQ=60)

                                                                        AGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGT                     <  SRR4302534.6389489/94‑1 (MQ=60)

                                                                        AGCGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGT                     <  SRR4302534.5006069/94‑1 (MQ=60)

                                                                          CGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTG                   >  SRR4302534.2102673/1‑94 (MQ=60)

                                                                          CGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTG                   <  SRR4302534.1964025/94‑1 (MQ=60)

                                                                          CGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTG                   >  SRR4302534.6225432/1‑94 (MQ=60)

                                                                          CGACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTG                   >  SRR4302534.191240/1‑94 (MQ=60)

                                                                           GACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGA                  <  SRR4302534.3898403/94‑1 (MQ=60)

                                                                            ACACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGAC                 <  SRR4302534.2847462/94‑1 (MQ=60)

                                                                             CACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACT                <  SRR4302534.1923180/94‑1 (MQ=60)

                                                                             CACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACT                <  SRR4302534.1009250/94‑1 (MQ=60)

                                                                             CACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACT                <  SRR4302534.4500792/94‑1 (MQ=60)

                                                                              ACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTT               <  SRR4302534.138502/94‑1 (MQ=60)

                                                                              ACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTT               <  SRR4302534.6073598/94‑1 (MQ=60)

                                                                              ACGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTT               <  SRR4302534.5973757/94‑1 (MQ=60)

                                                                               CGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTG              >  SRR4302534.3495710/1‑94 (MQ=60)

                                                                               CGCAGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTG              <  SRR4302534.5859717/94‑1 (MQ=60)

                                                                                  AGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCA           >  SRR4302534.368350/1‑94 (MQ=60)

                                                                                  AGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCA           <  SRR4302534.1927403/94‑1 (MQ=60)

                                                                                  AGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCA           >  SRR4302534.3104305/1‑94 (MQ=60)

                                                                                  AGTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTCCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCA           <  SRR4302534.6235931/94‑1 (MQ=60)

                                                                                   GTG‑CCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCAT          <  SRR4302534.1961711/94‑1 (MQ=60)

                                                                                     tgCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATA         <  SRR4302534.2036131/92‑1 (MQ=60)

                                                                                        CCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTA      <  SRR4302534.617019/94‑1 (MQ=60)

                                                                                        CCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTA      <  SRR4302534.5929524/94‑1 (MQ=60)

                                                                                        CCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTA      >  SRR4302534.2526880/1‑94 (MQ=60)

                                                                                        CCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTA      >  SRR4302534.5030947/1‑94 (MQ=60)

                                                                                         CCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAA     >  SRR4302534.6105288/1‑94 (MQ=60)

                                                                                          CCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAAT    <  SRR4302534.4626593/94‑1 (MQ=60)

                                                                                           CCCAGGCATAACGTTCATATCCCCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCGATATTAATG   <  SRR4302534.4694279/94‑1 (MQ=60)

                                                                                           CCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATG   >  SRR4302534.691236/1‑94 (MQ=60)

                                                                                           CCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGCTATGGATATTAATGTGTTGACTTGCCATATTAATG   >  SRR4302534.3842693/1‑94 (MQ=60)

                                                                                            CCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGG  <  SRR4302534.1535526/94‑1 (MQ=60)

                                                                                            CAAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGG  <  SRR4302534.4935386/94‑1 (MQ=60)

CCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGG  >  NC_000913/1592063‑1592248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`