Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,672,622	T→C	L229P (CTG→CCG)	b1601 →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,672,622	0	T	C	99.5%	734.6 / ‑4.4	210	L229P (CTG→CCG)	b1601	putative transport protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (104/105); total (104/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 215 total aligned reads displayed.

CTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCT  >  NC_000913/1672531‑1672711

  gAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGc                                                                                        >  2:1055311/1‑93 (MQ=255)

    aaaCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCt                                                                                      >  1:4343439/1‑93 (MQ=255)

    aaaCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCt                                                                                      <  2:3196228/93‑1 (MQ=255)

     aaCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCtt                                                                                     <  1:89735/93‑1 (MQ=255)

     aaCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCtt                                                                                     <  2:2176869/93‑1 (MQ=255)

     aaCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCtt                                                                                     <  2:6342762/93‑1 (MQ=255)

     aaCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCtt                                                                                     <  2:5388566/93‑1 (MQ=255)

     aaCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCtt                                                                                     <  1:3599799/93‑1 (MQ=255)

      aCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCttt                                                                                    <  1:2449096/93‑1 (MQ=255)

      aCAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCttt                                                                                    >  2:5625478/1‑93 (MQ=255)

      aCAGCCATCAGCATCAGCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCttt                                                                                    <  2:544045/93‑1 (MQ=255)

       cAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTg                                                                                   <  1:5541764/93‑1 (MQ=255)

       cAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTg                                                                                   <  1:3507008/93‑1 (MQ=255)

        aGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTgc                                                                                  <  1:2869386/93‑1 (MQ=255)

         gCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTgcg                                                                                 >  2:1480782/1‑93 (MQ=255)

          ccATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTgcgc                                                                                <  2:3058366/93‑1 (MQ=255)

           cATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCt                                                                               <  2:463170/93‑1 (MQ=255)

            aTCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCtt                                                                              >  2:6002247/1‑93 (MQ=255)

             tCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTa                                                                             >  2:1637198/1‑93 (MQ=255)

              cAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTaa                                                                            <  2:3750805/93‑1 (MQ=255)

               aGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAAt                                                                           >  2:1397153/1‑93 (MQ=255)

               aGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTCCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAAt                                                                           <  2:4526488/93‑1 (MQ=255)

                gCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTTAtt                                                                          >  1:2529962/1‑93 (MQ=255)

                gCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAAtt                                                                          <  1:2840131/93‑1 (MQ=255)

                gCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAAtt                                                                          >  1:3213585/1‑93 (MQ=255)

                   tcatcaCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACa                                                                       >  2:3792529/1‑93 (MQ=255)

                   tcatcaCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACa                                                                       <  1:559866/93‑1 (MQ=255)

                   tcatcaCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACa                                                                       >  1:1629314/1‑93 (MQ=255)

                    catcaCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACAt                                                                      <  2:6262778/93‑1 (MQ=255)

                      tcaCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATcc                                                                    >  1:3339148/1‑93 (MQ=255)

                      tcaCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATcc                                                                    <  1:1497093/93‑1 (MQ=255)

                      ccaCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATcc                                                                    <  1:1532238/92‑1 (MQ=255)

                        aCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCg                                                                  <  1:5817578/93‑1 (MQ=255)

                        aCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTGTGCGCTTAATTACATCCCg                                                                  >  1:5279191/1‑93 (MQ=255)

                         ccGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGa                                                                 >  1:341483/1‑93 (MQ=255)

                         ccGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGa                                                                 >  2:2023120/1‑93 (MQ=255)

                           ggCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAat                                                               >  1:1182173/1‑93 (MQ=255)

                             ccTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAatat                                                             <  1:4899933/93‑1 (MQ=255)

                              cTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATAtt                                                            <  1:5255374/93‑1 (MQ=255)

                              cTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATAtt                                                            >  2:4246455/1‑93 (MQ=255)

                              cTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATAtt                                                            <  1:6214810/93‑1 (MQ=255)

                               tGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTg                                                           <  1:5403896/93‑1 (MQ=255)

                               tGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTg                                                           >  2:3608434/1‑93 (MQ=255)

                               tGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTg                                                           >  1:808262/1‑93 (MQ=255)

                                tgTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTgg                                                          <  2:4050584/92‑1 (MQ=255)

                                ggTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTgg                                                          >  1:3054017/1‑93 (MQ=255)

                                 gTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGt                                                         <  2:1943705/93‑1 (MQ=255)

                                  tCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGtt                                                        <  2:6439599/93‑1 (MQ=255)

                                   cGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTc                                                       <  2:2748433/93‑1 (MQ=255)

                                   cGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTc                                                       <  1:1719847/93‑1 (MQ=255)

                                    gCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCa                                                      <  1:4715433/93‑1 (MQ=255)

                                     ccTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACCTCCCGAATATTGGTTCAg                                                     >  2:2379732/1‑93 (MQ=255)

                                      cTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGt                                                    >  1:3831183/1‑93 (MQ=255)

                                       tGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTc                                                   >  1:4468179/1‑93 (MQ=255)

                                       tGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTc                                                   <  2:5825116/93‑1 (MQ=255)

                                       tGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTc                                                   >  1:1880236/1‑93 (MQ=255)

                                       tGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTc                                                   >  1:1386912/1‑93 (MQ=255)

                                        gggCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGACTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTcc                                                  >  2:2089489/1‑93 (MQ=255)

                                         ggCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCt                                                 <  1:5862493/93‑1 (MQ=255)

                                         ggCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCt                                                 <  1:4538392/93‑1 (MQ=255)

                                         ggCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCt                                                 <  1:4222144/93‑1 (MQ=255)

                                            gATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTcgc                                              >  1:5734765/1‑93 (MQ=255)

                                            gATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTcgc                                              >  2:6030661/1‑93 (MQ=255)

                                            gATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTcgc                                              <  1:3099401/93‑1 (MQ=255)

                                            gATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTcgc                                              >  2:1384630/1‑93 (MQ=255)

                                             aTGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTcgcg                                             >  1:6092861/1‑93 (MQ=255)

                                               gCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGc                                           <  1:2637873/93‑1 (MQ=255)

                                                 tCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCaa                                         <  1:2041568/93‑1 (MQ=255)

                                                 tCGCCGCACTCGATGTTCGCTTCGCTTTTATCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCaa                                         >  2:5731642/1‑93 (MQ=255)

                                                  cgccgcACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAAt                                        <  2:4907334/93‑1 (MQ=255)

                                                    ccgcACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATcc                                      >  1:23716/1‑93 (MQ=255)

                                                     cgcACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATccc                                     >  1:1027494/1‑93 (MQ=255)

                                                     cgcACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATccc                                     >  2:6284955/1‑93 (MQ=255)

                                                        aCTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATcccccc                                  >  2:4952196/1‑93 (MQ=255)

                                                          tCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTa                                <  2:3332260/93‑1 (MQ=255)

                                                           cGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTAt                               <  2:6464458/93‑1 (MQ=255)

                                                           cGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTAt                               >  2:5676800/1‑93 (MQ=255)

                                                           cGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTAt                               <  1:4126530/93‑1 (MQ=255)

                                                            gATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATc                              <  2:2744828/93‑1 (MQ=255)

                                                            gATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATc                              <  2:4858817/93‑1 (MQ=255)

                                                            gATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATc                              >  2:5287006/1‑93 (MQ=255)

                                                            gATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATc                              >  2:2638492/1‑93 (MQ=255)

                                                             aTGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCACTCCCCCCTATCg                             >  1:2730906/1‑93 (MQ=255)

                                                             aTGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCg                             >  2:6061649/1‑93 (MQ=255)

                                                               gTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTTCTCGCGGCAATCCCCCCTATCGCt                           >  1:6311024/1‑93 (MQ=255)

                                                               gTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCt                           <  2:1116961/93‑1 (MQ=255)

                                                                 tcgcttcgctTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCa                         <  1:3666256/93‑1 (MQ=255)

                                                                 tcgcttcgctTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCa                         <  2:1421129/93‑1 (MQ=255)

                                                                      tcgcttTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTAc                    >  2:107022/1‑93 (MQ=255)

                                                                         gttttGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATCTTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTgg                 <  2:4169562/92‑1 (MQ=255)

                                                                          ttttGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGgt                <  2:3994264/93‑1 (MQ=255)

                                                                          ttttGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGgt                <  2:3052720/93‑1 (MQ=255)

                                                                            ttGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGgtgt              <  2:862614/93‑1 (MQ=255)

                                                                            ttGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGgtgt              >  1:6076936/1‑93 (MQ=255)

                                                                                 tGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAAt         >  2:589437/1‑93 (MQ=255)

                                                                                 tGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAAt         >  1:4301526/1‑93 (MQ=255)

                                                                                  ggggATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATg        >  1:3740339/1‑93 (MQ=255)

                                                                                  ggggATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATg        >  1:1062629/1‑93 (MQ=255)

                                                                                        tGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCt  <  1:5329505/93‑1 (MQ=255)

                                                                                        tGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCt  <  1:4613568/93‑1 (MQ=255)

CTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCT  >  NC_000913/1672531‑1672711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGA  >  NC_000913/1672529‑1672715

CGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCC                                                                                               >  SRR4302534.3300169/1‑94 (MQ=60)

CGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCC                                                                                               >  SRR4302534.228361/1‑94 (MQ=60)

CGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCC                                                                                               >  SRR4302534.1904793/1‑94 (MQ=60)

CGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCATGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCC                                                                                               <  SRR4302534.6651674/94‑1 (MQ=60)

 GCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCG                                                                                              <  SRR4302534.5816071/94‑1 (MQ=60)

  CTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGG                                                                                             <  SRR4302534.4768868/94‑1 (MQ=60)

  CTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGG                                                                                             >  SRR4302534.213142/1‑94 (MQ=60)

     AAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCT                                                                                          >  SRR4302534.4443265/1‑94 (MQ=60)

     AAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCT                                                                                          >  SRR4302534.4460760/1‑94 (MQ=60)

      AAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTT                                                                                         <  SRR4302534.1151817/94‑1 (MQ=60)

      AAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTT                                                                                         >  SRR4302534.272837/1‑94 (MQ=60)

      AAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTT                                                                                         <  SRR4302534.2477879/94‑1 (MQ=60)

      AAACACCCATCAGCATCATCCCCGGCCTGGTCGCCTGGGCGATGCACGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTT                                                                                         <  SRR4302534.2977840/94‑1 (MQ=60)

       AACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTT                                                                                        <  SRR4302534.92126/94‑1 (MQ=60)

       AACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTT                                                                                        <  SRR4302534.3696610/94‑1 (MQ=60)

       AACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTT                                                                                        <  SRR4302534.5044726/94‑1 (MQ=60)

        ACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGCTTGCCGGCCTTTG                                                                                       >  SRR4302534.1256806/1‑94 (MQ=60)

        ACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTG                                                                                       <  SRR4302534.2514175/94‑1 (MQ=60)

         CAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGC                                                                                      <  SRR4302534.3601351/94‑1 (MQ=60)

         CAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGC                                                                                      >  SRR4302534.5412930/1‑94 (MQ=60)

         CAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGC                                                                                      <  SRR4302534.5691359/94‑1 (MQ=60)

          AGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCG                                                                                     <  SRR4302534.2944729/94‑1 (MQ=60)

            CCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCT                                                                                   >  SRR4302534.5782599/1‑94 (MQ=60)

              ATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTA                                                                                 >  SRR4302534.882775/1‑94 (MQ=60)

                CAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAAT                                                                               <  SRR4302534.814304/94‑1 (MQ=60)

                 AGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTTATT                                                                              >  SRR4302534.2597083/1‑94 (MQ=60)

                 AGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATT                                                                              >  SRR4302534.3298864/1‑94 (MQ=60)

                  GCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTA                                                                             <  SRR4302534.4254513/94‑1 (MQ=60)

                  GCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTA                                                                             <  SRR4302534.2914742/94‑1 (MQ=60)

                   CATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTTTCTGGGGATTGCCGGCCTTTGCGCTTAATTAC                                                                            >  SRR4302534.2013858/1‑94 (MQ=60)

                   CATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTAC                                                                            >  SRR4302534.2985345/1‑94 (MQ=60)

                    ATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACA                                                                           >  SRR4302534.3387409/1‑94 (MQ=60)

                    ATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACA                                                                           >  SRR4302534.1671808/1‑94 (MQ=60)

                     TCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACAT                                                                          <  SRR4302534.573654/94‑1 (MQ=60)

                       ATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCC                                                                        >  SRR4302534.3428474/1‑94 (MQ=60)

                       ATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCC                                                                        >  SRR4302534.3736869/1‑94 (MQ=60)

                        TCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCC                                                                       <  SRR4302534.1536503/94‑1 (MQ=60)

                        CCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCC                                                                       <  SRR4302534.1572434/94‑1 (MQ=60)

                         CACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTGTGCGCTTAATTACATCCCG                                                                      >  SRR4302534.5421740/1‑94 (MQ=60)

                          ACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGA                                                                     <  SRR4302534.5975475/94‑1 (MQ=60)

                          ACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGA                                                                     >  SRR4302534.350216/1‑94 (MQ=60)

                            CGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAAT                                                                   >  SRR4302534.1213247/1‑94 (MQ=60)

                              GCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATAT                                                                 >  SRR4302534.1557642/1‑94 (MQ=60)

                               CCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATT                                                                <  SRR4302534.5604632/94‑1 (MQ=60)

                               CCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATT                                                                <  SRR4302534.5032785/94‑1 (MQ=60)

                                CTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTG                                                               <  SRR4302534.5397394/94‑1 (MQ=60)

                                CTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTG                                                               >  SRR4302534.828575/1‑94 (MQ=60)

                                CTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTG                                                               <  SRR4302534.3274044/94‑1 (MQ=60)

                                CTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTG                                                               <  SRR4302534.6383674/94‑1 (MQ=60)

                                CTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTG                                                               <  SRR4302534.6383673/94‑1 (MQ=60)

                                 TGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGG                                                              <  SRR4302534.4883240/94‑1 (MQ=60)

                                 TGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGG                                                              <  SRR4302534.5549328/94‑1 (MQ=60)

                                 TGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGG                                                              >  SRR4302534.3134141/1‑94 (MQ=60)

                                 TGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGG                                                              >  SRR4302534.3345628/1‑94 (MQ=60)

                                   GTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGCT                                                            <  SRR4302534.6371788/94‑1 (MQ=60)

                                    TCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTC                                                           <  SRR4302534.158450/94‑1 (MQ=60)

                                     CGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCA                                                          <  SRR4302534.1764517/94‑1 (MQ=60)

                                     CGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCA                                                          >  SRR4302534.1742458/1‑94 (MQ=60)

                                     CGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCA                                                          <  SRR4302534.3487448/94‑1 (MQ=60)

                                      GCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAG                                                         <  SRR4302534.4843479/94‑1 (MQ=60)

                                       CCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGT                                                        >  SRR4302534.3934077/1‑94 (MQ=60)

                                       CCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGT                                                        >  SRR4302534.469077/1‑94 (MQ=60)

                                        CTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTC                                                       >  SRR4302534.4589010/1‑94 (MQ=60)

                                        CTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTC                                                       >  SRR4302534.1929209/1‑94 (MQ=60)

                                        CTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTC                                                       >  SRR4302534.1423410/1‑94 (MQ=60)

                                         TGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCC                                                      >  SRR4302534.3175782/1‑94 (MQ=60)

                                         TGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCC                                                      <  SRR4302534.4508579/94‑1 (MQ=60)

                                          GGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCT                                                     <  SRR4302534.3838550/94‑1 (MQ=60)

                                           GGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTC                                                    <  SRR4302534.6021664/94‑1 (MQ=60)

                                           GGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTC                                                    <  SRR4302534.4661397/94‑1 (MQ=60)

                                           GGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTC                                                    <  SRR4302534.4770658/94‑1 (MQ=60)

                                           GGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTC                                                    <  SRR4302534.4783397/94‑1 (MQ=60)

                                           GGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTC                                                    <  SRR4302534.4335963/94‑1 (MQ=60)

                                           GGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTC                                                    <  SRR4302534.39235/94‑1 (MQ=60)

                                           GGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTC                                                    <  SRR4302534.2888350/94‑1 (MQ=60)

                                             CGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGC                                                  >  SRR4302534.5890168/1‑94 (MQ=60)

                                              GATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCG                                                 <  SRR4302534.3180926/94‑1 (MQ=60)

                                              GATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCG                                                 <  SRR4302534.1080867/94‑1 (MQ=60)

                                              GATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCG                                                 <  SRR4302534.2792302/94‑1 (MQ=60)

                                              GATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCG                                                 >  SRR4302534.6258547/1‑94 (MQ=60)

                                                 GCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCA                                              <  SRR4302534.2707657/94‑1 (MQ=60)

                                                  CTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAA                                             <  SRR4302534.6027638/94‑1 (MQ=60)

                                                   TCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAAT                                            <  SRR4302534.2094932/94‑1 (MQ=60)

                                                     GCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC                                          <  SRR4302534.1755422/94‑1 (MQ=60)

                                                     GCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCC                                          >  SRR4302534.24343/1‑94 (MQ=60)

                                                      CCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCC                                         >  SRR4302534.1053959/1‑94 (MQ=60)

                                                      CCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCAC                                         >  SRR4302534.5063989/1‑94 (MQ=60)

                                                        GCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCC                                       >  SRR4302534.5476396/1‑94 (MQ=60)

                                                          ACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCT                                     >  SRR4302534.1495160/1‑94 (MQ=60)

                                                             CGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATC                                  >  SRR4302534.396952/1‑94 (MQ=60)

                                                             CGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATC                                  <  SRR4302534.4237638/94‑1 (MQ=60)

                                                              GATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCACTCCCCCCTATCG                                 >  SRR4302534.2802912/1‑94 (MQ=60)

                                                              GATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCG                                 <  SRR4302534.3684035/94‑1 (MQ=60)

                                                              GATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCG                                 >  SRR4302534.4201945/1‑94 (MQ=60)

                                                                TGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTTCTCGCGGCAATCCCCCCTATCGCT                               >  SRR4302534.6481640/1‑94 (MQ=60)

                                                                TGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCT                               >  SRR4302534.5959811/1‑94 (MQ=60)

                                                                   TCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAG                            >  SRR4302534.4354534/1‑94 (MQ=60)

                                                                   TCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAG                            <  SRR4302534.3764790/94‑1 (MQ=60)

                                                                       TTCGCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTAC                        >  SRR4302534.6254938/1‑94 (MQ=60)

                                                                          GCTTTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGG                     >  SRR4302534.569959/1‑94 (MQ=60)

                                                                             TTTGTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGT                  >  SRR4302534.6242185/1‑94 (MQ=60)

                                                                                GTCTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTA               <  SRR4302534.5455657/94‑1 (MQ=60)

                                                                                  CTGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAAT             >  SRR4302534.4418130/1‑94 (MQ=60)

                                                                                   TGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATG            >  SRR4302534.3840835/1‑94 (MQ=60)

                                                                                   TGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATG            >  SRR4302534.1090172/1‑94 (MQ=60)

                                                                                   TGGGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATG            >  SRR4302534.2924351/1‑94 (MQ=60)

                                                                                     GGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGC          >  SRR4302534.1020788/1‑94 (MQ=60)

                                                                                     GGGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGC          >  SRR4302534.2575015/1‑94 (MQ=60)

                                                                                      GGATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCT         <  SRR4302534.5998002/94‑1 (MQ=60)

                                                                                       GATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTT        <  SRR4302534.4606485/94‑1 (MQ=60)

                                                                                       GATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTT        >  SRR4302534.2662189/1‑94 (MQ=60)

                                                                                       GATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTT        >  SRR4302534.554664/1‑94 (MQ=60)

                                                                                        ATTGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTC       <  SRR4302534.6338700/94‑1 (MQ=60)

                                                                                         TTGCCGGCCTTCGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCACGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCT      <  SRR4302534.3607181/94‑1 (MQ=60)

                                                                                          TGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTA     <  SRR4302534.5473242/94‑1 (MQ=60)

                                                                                          TGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTA     <  SRR4302534.1767221/94‑1 (MQ=60)

                                                                                          TGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTA     <  SRR4302534.4738778/94‑1 (MQ=60)

                                                                                          TGCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCACTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTA     <  SRR4302534.3537621/94‑1 (MQ=60)

                                                                                           GCCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTTC    >  SRR4302534.519868/1‑94 (MQ=60)

                                                                                            CCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACG   <  SRR4302534.5902519/94‑1 (MQ=60)

                                                                                            CCGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACG   >  SRR4302534.316807/1‑94 (MQ=60)

                                                                                             CGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGA  >  SRR4302534.945735/1‑94 (MQ=60)

TGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGTTCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGA  >  NC_000913/1672529‑1672715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`