Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,679,869 A→G S62S (AGT→AGC)  ydgC ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,679,8690AG99.6% 959.8 / ‑4.8 274S62S (AGT→AGC) ydgChypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (155/118);  total (155/119)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 283 total aligned reads displayed.

AGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGG  >  NC_000913/1679779‑1679958
                                                                                          |                                                                                         
 gAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGCAAACAAAATAGGGAATAATCGACCACATGCTa                                                                                        >  1:6148157/1‑93 (MQ=255)
   aCCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTaaa                                                                                      <  1:533895/93‑1 (MQ=255)
   aCCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTaaa                                                                                      >  1:4218650/1‑93 (MQ=255)
    ccAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTaaaa                                                                                     <  1:7066882/93‑1 (MQ=255)
     caacaaAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTaaaaa                                                                                    <  2:2278242/93‑1 (MQ=255)
      aacaaAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAat                                                                                   <  2:2907987/93‑1 (MQ=255)
      aacaaAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAat                                                                                   >  2:706048/1‑93 (MQ=255)
       acaaAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAatg                                                                                  >  2:5226550/1‑93 (MQ=255)
         aaaaGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAatgat                                                                                >  1:721251/1‑93 (MQ=255)
         aaaaGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAatgat                                                                                >  1:5154051/1‑93 (MQ=255)
          aaaGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAatgatg                                                                               >  2:4201100/1‑93 (MQ=255)
          aaaGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAatgatg                                                                               <  2:7865123/93‑1 (MQ=255)
          aaaGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCAAAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAatgatg                                                                               <  1:4562549/93‑1 (MQ=255)
           aaGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATgg                                                                              <  1:1291366/93‑1 (MQ=255)
           aaGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATgg                                                                              <  2:2582279/93‑1 (MQ=255)
            aGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGt                                                                             >  2:8708873/1‑93 (MQ=255)
            aGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGt                                                                             <  1:7392897/93‑1 (MQ=255)
              cggcggGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTg                                                                           <  2:4684575/93‑1 (MQ=255)
               ggcggGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTgc                                                                          >  1:2824353/1‑93 (MQ=255)
               ggcggGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTgc                                                                          <  2:6581155/93‑1 (MQ=255)
                gcggGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTgcg                                                                         <  2:3337868/93‑1 (MQ=255)
                gcggGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTgcg                                                                         <  2:6747012/93‑1 (MQ=255)
                   ggCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGcgcga                                                                      >  1:2664917/1‑92 (MQ=255)
                   ggCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGt                                                                      >  2:444234/1‑93 (MQ=255)
                   ggCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGt                                                                      >  1:3813026/1‑93 (MQ=255)
                   ggCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGt                                                                      >  2:4051795/1‑93 (MQ=255)
                    gCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTa                                                                     <  2:1089916/93‑1 (MQ=255)
                      aaGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAg                                                                   <  1:826443/93‑1 (MQ=255)
                      aaGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAg                                                                   >  2:1041108/1‑93 (MQ=255)
                       aGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAgg                                                                  <  1:8548014/93‑1 (MQ=255)
                       aGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAgg                                                                  >  2:3978022/1‑93 (MQ=255)
                        gcgcATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGc                                                                 >  2:3068387/1‑93 (MQ=255)
                        gcgcATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAAGAGGGAGTAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGc                                                                 <  1:6386089/93‑1 (MQ=255)
                          gcATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCtt                                                               >  2:1711571/1‑93 (MQ=255)
                          gcATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCtt                                                               >  2:8300602/1‑93 (MQ=255)
                          gcATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCtt                                                               <  1:7662470/93‑1 (MQ=255)
                          gcATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCtt                                                               >  2:5033664/1‑93 (MQ=255)
                            atcatTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCa                                                             >  2:8495019/1‑93 (MQ=255)
                            atcatTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCa                                                             >  1:4396026/1‑93 (MQ=255)
                             tcatTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCaa                                                            >  2:5496066/1‑93 (MQ=255)
                             tcatTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCaa                                                            >  2:2111668/1‑93 (MQ=255)
                             tcatTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCaa                                                            >  1:2313709/1‑93 (MQ=255)
                               cttCGGGTGAAATACGACAGCTACACCAGGTAAACAAAATAGGGAATAATCGCCCACATGCTAAAAATGCTGGTTGCGCGTAAGGCTTAAATg                                                          <  2:3176098/92‑1 (MQ=255)
                               atTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATg                                                          >  1:4630924/1‑93 (MQ=255)
                                  ccGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCcg                                                       <  1:6707934/93‑1 (MQ=255)
                                   cGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCcgc                                                      >  1:2027825/1‑93 (MQ=255)
                                   cGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCcgc                                                      <  2:1866890/93‑1 (MQ=255)
                                   cGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCcgc                                                      >  2:1274328/1‑93 (MQ=255)
                                   cGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCcgc                                                      >  1:8439125/1‑93 (MQ=255)
                                      tGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGtt                                                   <  2:8263133/93‑1 (MQ=255)
                                      tGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGtt                                                   <  1:1990104/93‑1 (MQ=255)
                                      tGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGtt                                                   <  1:1454563/93‑1 (MQ=255)
                                       gAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTc                                                  <  1:4657051/93‑1 (MQ=255)
                                       gAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTc                                                  <  2:346239/93‑1 (MQ=255)
                                         aaTACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGc                                                <  1:2717550/93‑1 (MQ=255)
                                           tACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTg                                              >  2:1389406/1‑93 (MQ=255)
                                            aCCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTgg                                             <  1:3274907/93‑1 (MQ=255)
                                              cacaGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGca                                           >  1:4168367/1‑93 (MQ=255)
                                               acaGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGcaa                                          >  1:5591749/1‑93 (MQ=255)
                                                caGCGACGCCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGcaac                                         <  2:6261172/93‑1 (MQ=255)
                                                   cGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAat                                      <  2:5897725/93‑1 (MQ=255)
                                                    gACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAata                                     <  1:2885965/93‑1 (MQ=255)
                                                     acacCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAatat                                    <  2:2065251/93‑1 (MQ=255)
                                                     acacCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAatat                                    >  2:7326871/1‑93 (MQ=255)
                                                       acCAGGTAAACAAAATAGGGAATAATCAACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATaa                                  >  2:6716142/1‑93 (MQ=255)
                                                        ccAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAAt                                 >  1:6843419/1‑93 (MQ=255)
                                                         cAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATg                                <  2:8771985/93‑1 (MQ=255)
                                                         cAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATg                                >  1:8774460/1‑93 (MQ=255)
                                                          aGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATgc                               <  1:2117906/93‑1 (MQ=255)
                                                          aGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGT‑AGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATgcg                              >  2:8106712/1‑93 (MQ=255)
                                                              aaaCAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGa                           >  1:1013359/1‑93 (MQ=255)
                                                               aaCAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGAt                          <  2:2810000/93‑1 (MQ=255)
                                                               aaCAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGAt                          >  1:3061059/1‑93 (MQ=255)
                                                               aaCAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGAt                          >  1:3383143/1‑93 (MQ=255)
                                                               aaCAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGAt                          >  1:4612399/1‑93 (MQ=255)
                                                                  aaaaTAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAg                       >  2:7490062/1‑93 (MQ=255)
                                                                  aaaaTAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAg                       <  1:5424312/93‑1 (MQ=255)
                                                                   aaaTAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAgc                      <  1:4775233/93‑1 (MQ=255)
                                                                    aaTAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAgcg                     >  1:6084332/1‑93 (MQ=255)
                                                                      tAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCa                   >  1:7592147/1‑93 (MQ=255)
                                                                        gggAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCaaa                 >  2:436296/1‑93 (MQ=255)
                                                                         ggAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAg                <  2:694409/93‑1 (MQ=255)
                                                                          gAATAATCGACCACATGCTAAAAATGATGGTTGCACGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAgg               <  1:5646843/93‑1 (MQ=255)
                                                                           aataatCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGt              >  2:3712865/1‑93 (MQ=255)
                                                                           aataatCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGt              <  2:3909981/93‑1 (MQ=255)
                                                                             taatCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCg            >  1:6526937/1‑93 (MQ=255)
                                                                              aatCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCgg           >  1:5934418/1‑93 (MQ=255)
                                                                              aatCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCgg           >  1:4864988/1‑93 (MQ=255)
                                                                               atCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCggg          >  2:8408433/1‑93 (MQ=255)
                                                                                  gACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGaaa       <  1:4054247/93‑1 (MQ=255)
                                                                                   aCCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGaaaa      >  2:2962735/1‑93 (MQ=255)
                                                                                   aCCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGaaaa      >  2:439693/1‑93 (MQ=255)
                                                                                   aCCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGaaaa      >  2:610730/1‑93 (MQ=255)
                                                                                   aCCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGaaaa      <  1:8028793/93‑1 (MQ=255)
                                                                                    ccACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAg     <  1:8138088/93‑1 (MQ=255)
                                                                                     cacaTGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGt    >  2:6549219/1‑93 (MQ=255)
                                                                                     cacaTGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGt    <  2:1171899/93‑1 (MQ=255)
                                                                                     cacaTGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGt    <  1:1624076/93‑1 (MQ=255)
                                                                                     cacaTGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGt    <  1:8184323/93‑1 (MQ=255)
                                                                                      acaTGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTg   <  1:8489471/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGG  >  NC_000913/1679779‑1679958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATC  >  NC_000913/1679776‑1679962
                                                                                             |                                                                                             
AACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATG                                                                                               <  SRR4302541.6465240/94‑1 (MQ=60)
  CAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCT                                                                                             <  SRR4302541.1163554/94‑1 (MQ=60)
  CAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCT                                                                                             >  SRR4302541.4155924/1‑94 (MQ=60)
  CAGAACCAACAAAAGCAGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCT                                                                                             <  SRR4302541.4947869/94‑1 (MQ=60)
   AGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTA                                                                                            <  SRR4302541.3110685/94‑1 (MQ=60)
   AGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGCAAACAAAATAGGGAATAATCGACCACATGCTA                                                                                            >  SRR4302541.6210975/1‑94 (MQ=60)
     AACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAA                                                                                          >  SRR4302541.4260635/1‑94 (MQ=60)
      ACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAA                                                                                         >  SRR4302541.1228385/1‑94 (MQ=60)
      ACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAA                                                                                         <  SRR4302541.8341262/94‑1 (MQ=60)
      ACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAA                                                                                         <  SRR4302541.538902/94‑1 (MQ=60)
       CCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAA                                                                                        <  SRR4302541.7142038/94‑1 (MQ=60)
         AACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATG                                                                                      <  SRR4302541.3311684/94‑1 (MQ=60)
         AACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATG                                                                                      <  SRR4302541.7090574/94‑1 (MQ=60)
         AACAAAAGCGGCGGGCAAGCGCATCATTCCGGGGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATG                                                                                      >  SRR4302541.7476241/1‑94 (MQ=60)
           CAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGAT                                                                                    <  SRR4302541.5874310/94‑1 (MQ=60)
           CAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGAT                                                                                    >  SRR4302541.5207076/1‑94 (MQ=60)
           CAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGAT                                                                                    >  SRR4302541.728070/1‑94 (MQ=60)
            AAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATG                                                                                   >  SRR4302541.6172806/1‑94 (MQ=60)
            AAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATG                                                                                   >  SRR4302541.1370203/1‑94 (MQ=60)
             AAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGG                                                                                  >  SRR4302541.2894209/1‑94 (MQ=60)
             AAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCAAAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGG                                                                                  <  SRR4302541.4608736/94‑1 (MQ=60)
              AAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGT                                                                                 <  SRR4302541.1304122/94‑1 (MQ=60)
              AAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGT                                                                                 >  SRR4302541.8543018/1‑94 (MQ=60)
              AAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGAGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAACTGATGGT                                                                                 >  SRR4302541.5926377/1‑94 (MQ=60)
               AGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTT                                                                                <  SRR4302541.7471952/94‑1 (MQ=60)
               AGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTT                                                                                <  SRR4302541.7614415/94‑1 (MQ=60)
                GCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTG                                                                               <  SRR4302541.717963/94‑1 (MQ=60)
                 CGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGC                                                                              >  SRR4302541.4241927/1‑94 (MQ=60)
                 CGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGC                                                                              >  SRR4302541.2721919/1‑94 (MQ=60)
                 CGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGC                                                                              <  SRR4302541.7798139/94‑1 (MQ=60)
                 CGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGC                                                                              >  SRR4302541.2852840/1‑94 (MQ=60)
                  GGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCG                                                                             >  SRR4302541.7391/1‑94 (MQ=60)
                     GGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGT                                                                          >  SRR4302541.4857183/1‑94 (MQ=60)
                     GGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGT                                                                          >  SRR4302541.8778748/1‑94 (MQ=60)
                     GGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGT                                                                          >  SRR4302541.1845105/1‑94 (MQ=60)
                     GGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGT                                                                          <  SRR4302541.3376897/94‑1 (MQ=60)
                     GGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGT                                                                          >  SRR4302541.3851431/1‑94 (MQ=60)
                     GGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGA                                                                          >  SRR4302541.2691413/1‑94 (MQ=60)
                        CAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAG                                                                       <  SRR4302541.5470863/94‑1 (MQ=60)
                         AAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGG                                                                      <  SRR4302541.833962/94‑1 (MQ=60)
                         AAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGG                                                                      >  SRR4302541.393578/1‑94 (MQ=60)
                         AAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGG                                                                      <  SRR4302541.5033731/94‑1 (MQ=60)
                          AGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGC                                                                     <  SRR4302541.8639894/94‑1 (MQ=60)
                           GCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAAGAGGGAGTAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCT                                                                    <  SRR4302541.6451301/94‑1 (MQ=60)
                            CGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTT                                                                   <  SRR4302541.4467373/94‑1 (MQ=60)
                             GCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTC                                                                  <  SRR4302541.2137248/94‑1 (MQ=60)
                             GCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTC                                                                  >  SRR4302541.2036815/1‑94 (MQ=60)
                             GCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTC                                                                  <  SRR4302541.4700233/94‑1 (MQ=60)
                             GCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTC                                                                  <  SRR4302541.7744016/94‑1 (MQ=60)
                              CATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCA                                                                 >  SRR4302541.4440000/1‑94 (MQ=60)
                               ATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAA                                                                >  SRR4302541.2336158/1‑94 (MQ=60)
                                TCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAAT                                                               <  SRR4302541.8893521/94‑1 (MQ=60)
                                TCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAAT                                                               >  SRR4302541.8262523/1‑94 (MQ=60)
                                 CATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATG                                                              >  SRR4302541.4678045/1‑94 (MQ=60)
                                 CATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATG                                                              >  SRR4302541.4678044/1‑94 (MQ=60)
                                 CATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATG                                                              >  SRR4302541.4678042/1‑94 (MQ=60)
                                  ATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGC                                                             >  SRR4302541.6158411/1‑94 (MQ=60)
                                   TTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATTCTAAAAATTATGGTTGCGCGTAAGGCTTCAATGCC                                                            <  SRR4302541.4847717/94‑1 (MQ=60)
                                     CCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGC                                                          >  SRR4302541.8530280/1‑94 (MQ=60)
                                     CCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGC                                                          <  SRR4302541.6287695/94‑1 (MQ=60)
                                     CCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGC                                                          >  SRR4302541.2047418/1‑94 (MQ=60)
                                     CCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGC                                                          <  SRR4302541.6778814/94‑1 (MQ=60)
                                      CGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCG                                                         <  SRR4302541.2240948/94‑1 (MQ=60)
                                        GTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTT                                                       <  SRR4302541.2467138/94‑1 (MQ=60)
                                         TGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTC                                                      <  SRR4302541.1468763/94‑1 (MQ=60)
                                         TGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTC                                                      <  SRR4302541.2009349/94‑1 (MQ=60)
                                         TGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTC                                                      <  SRR4302541.3507107/94‑1 (MQ=60)
                                          GAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCG                                                     <  SRR4302541.5655286/94‑1 (MQ=60)
                                          GAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCG                                                     <  SRR4302541.4704470/94‑1 (MQ=60)
                                          GAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCG                                                     <  SRR4302541.4704471/94‑1 (MQ=60)
                                            AATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCT                                                   <  SRR4302541.2744656/94‑1 (MQ=60)
                                              TACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGG                                                 >  SRR4302541.8477223/1‑94 (MQ=60)
                                               ACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGC                                                <  SRR4302541.3307939/94‑1 (MQ=60)
                                                CCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCA                                               >  SRR4302541.4209666/1‑94 (MQ=60)
                                                CCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCA                                               >  SRR4302541.4209665/1‑94 (MQ=60)
                                                CCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCA                                               <  SRR4302541.6452680/94‑1 (MQ=60)
                                                 CACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAA                                              >  SRR4302541.4279107/1‑94 (MQ=60)
                                                 CACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAA                                              >  SRR4302541.3007955/1‑94 (MQ=60)
                                                 CACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAA                                              >  SRR4302541.5648990/1‑94 (MQ=60)
                                                   CAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCCACA                                            >  SRR4302541.1895553/1‑94 (MQ=60)
                                                   CAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACA                                            >  SRR4302541.7118806/1‑94 (MQ=60)
                                                    AGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAA                                           >  SRR4302541.2011270/1‑94 (MQ=60)
                                                      CGACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATA                                         <  SRR4302541.5386242/94‑1 (MQ=60)
                                                       GACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATAT                                        >  SRR4302541.8284986/1‑94 (MQ=60)
                                                       GACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATAT                                        >  SRR4302541.8262597/1‑94 (MQ=60)
                                                       GACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATAT                                        <  SRR4302541.2914948/94‑1 (MQ=60)
                                                       GACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATAT                                        <  SRR4302541.8647704/94‑1 (MQ=60)
                                                        ACACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATA                                       >  SRR4302541.274101/1‑94 (MQ=60)
                                                        ACACCAGGTAAAAAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATA                                       <  SRR4302541.6880348/94‑1 (MQ=60)
                                                         CACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAA                                      <  SRR4302541.426131/94‑1 (MQ=60)
                                                          ACCAGGTAAACAAAATAGGGAATAATCGACGACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAAT                                     <  SRR4302541.238746/94‑1 (MQ=60)
                                                          ACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAAT                                     <  SRR4302541.7709706/94‑1 (MQ=60)
                                                          ACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAAT                                     >  SRR4302541.4053169/1‑94 (MQ=60)
                                                          ACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAAT                                     <  SRR4302541.7833532/94‑1 (MQ=60)
                                                          ACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAAT                                     >  SRR4302541.8626601/1‑94 (MQ=60)
                                                          ACCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAAT                                     >  SRR4302541.6916593/1‑94 (MQ=60)
                                                           CCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATG                                    >  SRR4302541.8868253/1‑94 (MQ=60)
                                                           CCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATG                                    >  SRR4302541.1592146/1‑94 (MQ=60)
                                                           CCAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATG                                    >  SRR4302541.4750641/1‑94 (MQ=60)
                                                            CAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGC                                   >  SRR4302541.6501482/1‑94 (MQ=60)
                                                            CAGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGC                                   >  SRR4302541.8468537/1‑94 (MQ=60)
                                                             ATGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCG                                  <  SRR4302541.8167733/94‑1 (MQ=60)
                                                             AGGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCG                                  <  SRR4302541.2138413/94‑1 (MQ=60)
                                                              GGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGC                                 >  SRR4302541.5548189/1‑94 (MQ=60)
                                                              GGTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGC                                 >  SRR4302541.5548188/1‑94 (MQ=60)
                                                               GTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCG                                <  SRR4302541.6230649/94‑1 (MQ=60)
                                                               GTAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCG                                <  SRR4302541.2738107/94‑1 (MQ=60)
                                                                TAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGA                               >  SRR4302541.1022732/1‑94 (MQ=60)
                                                                TAAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGA                               >  SRR4302541.2895618/1‑94 (MQ=60)
                                                                 AAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGAT                              >  SRR4302541.3092071/1‑94 (MQ=60)
                                                                 AAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGAT                              >  SRR4302541.3416868/1‑94 (MQ=60)
                                                                 AAACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGAT                              >  SRR4302541.4659254/1‑94 (MQ=60)
                                                                  AACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATA                             >  SRR4302541.142639/1‑94 (MQ=60)
                                                                  AACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATA                             <  SRR4302541.99015/94‑1 (MQ=60)
                                                                  AACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATA                             >  SRR4302541.5057640/1‑94 (MQ=60)
                                                                   ACAAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAA                            <  SRR4302541.4903477/94‑1 (MQ=60)
                                                                     AAAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGC                          <  SRR4302541.5479968/94‑1 (MQ=60)
                                                                      AAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCG                         <  SRR4302541.4504451/94‑1 (MQ=60)
                                                                      AAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCG                         <  SRR4302541.4823987/94‑1 (MQ=60)
                                                                      AAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCG                         >  SRR4302541.2254739/1‑94 (MQ=60)
                                                                      AAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCG                         >  SRR4302541.6146438/1‑94 (MQ=60)
                                                                      AAATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCG                         >  SRR4302541.1629356/1‑94 (MQ=60)
                                                                       AATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGC                        >  SRR4302541.6111651/1‑94 (MQ=60)
                                                                        ATAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCA                       >  SRR4302541.7673251/1‑94 (MQ=60)
                                                                         TAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAA                      <  SRR4302541.6704401/94‑1 (MQ=60)
                                                                         TAGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAA                      >  SRR4302541.6885363/1‑94 (MQ=60)
                                                                          AGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAA                     >  SRR4302541.5460674/1‑94 (MQ=60)
                                                                          AGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAA                     >  SRR4302541.461049/1‑94 (MQ=60)
                                                                          AGGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAA                     >  SRR4302541.461050/1‑94 (MQ=60)
                                                                           GGGAATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAG                    >  SRR4302541.8199992/1‑94 (MQ=60)
                                                                             GAATAATCGACCACATGCTAAAAATGATGGTTGCACGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGT                  <  SRR4302541.5704548/94‑1 (MQ=60)
                                                                               ATAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCG                >  SRR4302541.6593554/1‑94 (MQ=60)
                                                                                TAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGG               >  SRR4302541.4914617/1‑94 (MQ=60)
                                                                                TAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGG               <  SRR4302541.2786723/94‑1 (MQ=60)
                                                                                TAATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGG               >  SRR4302541.5994868/1‑94 (MQ=60)
                                                                                 AATCGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGG              <  SRR4302541.6073079/94‑1 (MQ=60)
                                                                                    CGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAA           >  SRR4302541.3217900/1‑94 (MQ=60)
                                                                                    CGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAA           >  SRR4302541.730645/1‑94 (MQ=60)
                                                                                    CGACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAA           >  SRR4302541.7185150/1‑94 (MQ=60)
                                                                                     GACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAA          >  SRR4302541.3652000/1‑94 (MQ=60)
                                                                                     GACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAA          <  SRR4302541.4094248/94‑1 (MQ=60)
                                                                                      ACCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAG         <  SRR4302541.8117061/94‑1 (MQ=60)
                                                                                       CCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGT        <  SRR4302541.8227141/94‑1 (MQ=60)
                                                                                       CCACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGG        >  SRR4302541.5282930/1‑94 (MQ=60)
                                                                                        CACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTG       <  SRR4302541.8273671/94‑1 (MQ=60)
                                                                                        CACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTG       <  SRR4302541.1640174/94‑1 (MQ=60)
                                                                                        CACATGCTAAAAATGATGGTTGCGCGTAAGGCTGCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTG       <  SRR4302541.8615479/94‑1 (MQ=60)
                                                                                         ACATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGG      <  SRR4302541.8580953/94‑1 (MQ=60)
                                                                                           ATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAA    >  SRR4302541.5290490/1‑94 (MQ=60)
                                                                                           ATGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGATCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAA    >  SRR4302541.6650381/1‑94 (MQ=60)
                                                                                            TGCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAAT   <  SRR4302541.1627085/94‑1 (MQ=60)
                                                                                             GCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATC  <  SRR4302541.474509/94‑1 (MQ=60)
                                                                                             |                                                                                             
AACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAATAGGGAATAATCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATC  >  NC_000913/1679776‑1679962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑