Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,728,403	T→C	R431R (CGT→CGC)	lhr →	Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,728,403	0	T	C	100.0%	663.4 / NA	197	R431R (CGT→CGC)	lhr	Lhr
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (101/96); total (101/96)

Only 100 of 200 total aligned reads displayed.

AACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGTACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCT  >  NC_000913/1728313‑1728492

aaCGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAc                                                                                         <  2:2932511/93‑1 (MQ=255)

aaCGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAc                                                                                         >  2:2167981/1‑93 (MQ=255)

aaCGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAc                                                                                         >  1:211683/1‑93 (MQ=255)

aaCGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAc                                                                                         >  1:2308830/1‑93 (MQ=255)

 aCGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAcc                                                                                        >  2:6278786/1‑93 (MQ=255)

 aCGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAcc                                                                                        >  2:168581/1‑93 (MQ=255)

  cgccgcTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAccc                                                                                       <  1:5669359/93‑1 (MQ=255)

  cgccgcTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAccc                                                                                       >  1:1370439/1‑93 (MQ=255)

   gccgcTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCg                                                                                      >  2:5157088/1‑93 (MQ=255)

     cgcTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGc                                                                                    <  2:767573/93‑1 (MQ=255)

     cgcTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGc                                                                                    <  2:4718810/93‑1 (MQ=255)

       cTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGt                                                                                  <  2:3244998/93‑1 (MQ=255)

        cttCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTg                                                                                 <  1:3169005/92‑1 (MQ=255)

         ttCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGa                                                                                <  1:3371645/93‑1 (MQ=255)

         ttCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGa                                                                                <  2:911517/93‑1 (MQ=255)

          tCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGAt                                                                               <  1:713592/93‑1 (MQ=255)

           cTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGAtt                                                                              <  1:6198558/93‑1 (MQ=255)

            tGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGAttt                                                                             >  1:507761/1‑93 (MQ=255)

            tGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGAttt                                                                             >  2:3844638/1‑93 (MQ=255)

            tGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTGCCCCCGCACCCGGCGTGAttt                                                                             <  1:3015235/93‑1 (MQ=255)

               tGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGt                                                                          <  2:354384/93‑1 (MQ=255)

                gCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTc                                                                         <  1:4588685/93‑1 (MQ=255)

                  cAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGa                                                                       <  2:5456293/93‑1 (MQ=255)

                  cAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGa                                                                       >  1:4873241/1‑93 (MQ=255)

                   aGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGAt                                                                      >  1:1089820/1‑93 (MQ=255)

                     tGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTc                                                                    >  1:1312920/1‑93 (MQ=255)

                      gggTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTcc                                                                   >  1:4052664/1‑93 (MQ=255)

                          tACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAg                                                               <  2:2409357/93‑1 (MQ=255)

                          tACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAg                                                               >  2:5305552/1‑93 (MQ=255)

                           aCAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGt                                                              >  2:6329586/1‑93 (MQ=255)

                           aCAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGt                                                              >  2:4906765/1‑93 (MQ=255)

                             aaCGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCa                                                            <  2:5735040/93‑1 (MQ=255)

                              aCGCATTGATCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTCGTCGATTCCGCAGTCAt                                                           >  1:6306473/1‑93 (MQ=255)

                               cGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCAtt                                                          <  1:4565123/93‑1 (MQ=255)

                               cGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCAtt                                                          <  2:309074/93‑1 (MQ=255)

                               cGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCAtt                                                          >  2:2243699/1‑93 (MQ=255)

                               cGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCAtt                                                          <  2:6457975/93‑1 (MQ=255)

                                gCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCCTTg                                                         >  1:6443936/1‑93 (MQ=255)

                                  aTTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTa                                                       >  2:4587819/1‑93 (MQ=255)

                                  aTTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTa                                                       >  1:5962220/1‑93 (MQ=255)

                                    tGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTaga                                                     <  2:636053/93‑1 (MQ=255)

                                     ggTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTagag                                                    >  1:3671985/1‑93 (MQ=255)

                                      gTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAgt                                                   <  2:1345031/93‑1 (MQ=255)

                                      gTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAgt                                                   >  2:3815472/1‑93 (MQ=255)

                                           gcCGGACATCAGGTTGGCGGTGTATCGAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATg                                              <  1:6153683/93‑1 (MQ=255)

                                            ccGGACATCAGGTTGGCTGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGt                                             <  1:3697504/93‑1 (MQ=255)

                                            ccGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGt                                             >  2:4401186/1‑93 (MQ=255)

                                            ccGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGt                                             >  1:194114/1‑93 (MQ=255)

                                            ccGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGt                                             <  2:1826271/93‑1 (MQ=255)

                                             cGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGtt                                            >  2:1253864/1‑93 (MQ=255)

                                             cGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGtt                                            >  2:6188783/1‑93 (MQ=255)

                                              ggACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTc                                           <  2:1979580/93‑1 (MQ=255)

                                               gACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCCCCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCg                                          <  2:3373720/93‑1 (MQ=255)

                                               gACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCg                                          >  2:6152173/1‑93 (MQ=255)

                                                 cATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCgca                                        >  1:5136219/1‑93 (MQ=255)

                                                 cATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCgca                                        <  2:2576717/93‑1 (MQ=255)

                                                 cATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCgca                                        <  2:782031/93‑1 (MQ=255)

                                                  aTCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCgcag                                       <  2:2412143/93‑1 (MQ=255)

                                                   tCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCgcagg                                      <  2:1718461/93‑1 (MQ=255)

                                                   tCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCgcagg                                      >  2:4766364/1‑93 (MQ=255)

                                                      ggTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCgcaggcag                                   >  1:1619372/1‑93 (MQ=255)

                                                      ggTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCgcaggcag                                   >  2:5790854/1‑93 (MQ=255)

                                                       gTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGcaggcagg                                  <  1:4859176/93‑1 (MQ=255)

                                                        ttGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCaggcaggc                                 <  2:5005480/93‑1 (MQ=255)

                                                        ttGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCaggcaggc                                 <  1:2672517/93‑1 (MQ=255)

                                                         tGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCt                                <  1:5728369/93‑1 (MQ=255)

                                                         tGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCt                                >  1:4800870/1‑93 (MQ=255)

                                                          ggCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTg                               >  2:4474314/1‑93 (MQ=255)

                                                          ggCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTg                               >  2:929652/1‑93 (MQ=255)

                                                            cGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGa                             >  1:2652129/1‑93 (MQ=255)

                                                            cGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGa                             <  2:3489534/93‑1 (MQ=255)

                                                                gtATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAAc                         >  1:3213731/1‑93 (MQ=255)

                                                                 tATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAAcc                        <  2:4339471/93‑1 (MQ=255)

                                                                  aTCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCt                       <  2:2724185/93‑1 (MQ=255)

                                                                   tCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTg                      <  2:6090224/93‑1 (MQ=255)

                                                                    cTAAAGGGCTGTTTTTCCCCCGCACCCGGCGGGATTTAGTCGATTCCGCAGTCACTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGa                     >  2:1529536/1‑93 (MQ=255)

                                                                     tAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGac                    <  2:1874990/93‑1 (MQ=255)

                                                                       aaGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGacac                  >  2:5664932/1‑93 (MQ=255)

                                                                        aGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGCcacc                 >  1:1475886/1‑93 (MQ=255)

                                                                         gggCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGAcacca                >  2:6025186/1‑93 (MQ=255)

                                                                         gggCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGAcacca                >  1:5700816/1‑93 (MQ=255)

                                                                          ggCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGAcaccac               >  1:4257589/1‑93 (MQ=255)

                                                                             tGTTTTTCCCCCGCACCCGGCGGGATTTAGTCGATTTCGCAGTCATTGTAGAGTGGATGTTCGCAGGCAGGCTGGAAAACCCGACAACACCGc            >  2:1379131/1‑93 (MQ=255)

                                                                              gTTTTTCCCCCGCACCCTGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTAGCAGGCAGGCTGGAAAACCTGACACCAACGcc           >  2:1169300/1‑92 (MQ=255)

                                                                              gTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAAACTGACACCACCGCa           >  1:3482871/1‑93 (MQ=255)

                                                                              gTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGGGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCa           >  1:2916722/1‑93 (MQ=255)

                                                                               tttttCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACCCCACCGCAt          <  2:2769625/93‑1 (MQ=255)

                                                                               tttttCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCAt          <  1:1189440/93‑1 (MQ=255)

                                                                               tttttCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCAACGCaa          >  1:2488428/1‑92 (MQ=255)

                                                                                ttttCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATa         >  2:4243133/1‑93 (MQ=255)

                                                                                    cccccGCGCCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATcc     <  1:3950543/93‑1 (MQ=255)

                                                                                     ccccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCct    >  1:5508583/1‑93 (MQ=255)

                                                                                     ccccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCct    >  2:379507/1‑93 (MQ=255)

                                                                                      cccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCctc   <  2:5595810/93‑1 (MQ=255)

                                                                                      cccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCctc   <  1:3135397/93‑1 (MQ=255)

                                                                                      cccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCctc   <  1:957867/93‑1 (MQ=255)

                                                                                       ccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCctct  >  1:5001126/1‑93 (MQ=255)

                                                                                       ccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCctct  <  1:2053806/93‑1 (MQ=255)

                                                                                       ccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCctct  >  2:891893/1‑93 (MQ=255)

                                                                                       ccGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCctct  >  2:3728068/1‑93 (MQ=255)

AACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGTACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCT  >  NC_000913/1728313‑1728492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGTACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGAC  >  NC_000913/1728311‑1728496

GCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCA                                                                                              <  SRR4302534.2219725/94‑1 (MQ=60)

 TAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAC                                                                                             >  SRR4302534.217233/1‑94 (MQ=60)

 CAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAC                                                                                             >  SRR4302534.4919737/1‑94 (MQ=60)

 CAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAC                                                                                             >  SRR4302534.2370116/1‑94 (MQ=60)

 CAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCAC                                                                                             >  SRR4302534.3237040/1‑94 (MQ=60)

  AACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACC                                                                                            >  SRR4302534.3320066/1‑94 (MQ=60)

   ACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCC                                                                                           >  SRR4302534.1406554/1‑94 (MQ=60)

    CGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCG                                                                                          <  SRR4302534.5822856/94‑1 (MQ=60)

    CGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCG                                                                                          <  SRR4302534.2861712/94‑1 (MQ=60)

       CGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCTCGCACCCGGCG                                                                                       >  SRR4302534.5151191/1‑94 (MQ=60)

        GCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGT                                                                                      >  SRR4302534.6361023/1‑94 (MQ=60)

         CTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTG                                                                                     <  SRR4302534.2567623/94‑1 (MQ=60)

          TTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGA                                                                                    <  SRR4302534.5804205/94‑1 (MQ=60)

          TTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGA                                                                                    <  SRR4302534.3297831/94‑1 (MQ=60)

          CTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGA                                                                                    <  SRR4302534.3252593/94‑1 (MQ=60)

           TTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGAT                                                                                   <  SRR4302534.3461984/94‑1 (MQ=60)

            TCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATT                                                                                  <  SRR4302534.731144/94‑1 (MQ=60)

            TCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATT                                                                                  <  SRR4302534.5880321/94‑1 (MQ=60)

             CTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTT                                                                                 >  SRR4302534.520398/1‑94 (MQ=60)

             CTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTT                                                                                 <  SRR4302534.6367173/94‑1 (MQ=60)

              TGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTGCCCCCGCACCCGGCGTGATTTA                                                                                <  SRR4302534.3094164/94‑1 (MQ=60)

               GTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAG                                                                               >  SRR4302534.4986962/1‑94 (MQ=60)

                  GCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCG                                                                            <  SRR4302534.4713208/94‑1 (MQ=60)

                   CCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGA                                                                           >  SRR4302534.5005413/1‑94 (MQ=60)

                    CAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGAT                                                                          >  SRR4302534.1118174/1‑94 (MQ=60)

                    CAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGAT                                                                          >  SRR4302534.2545764/1‑94 (MQ=60)

                     AGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATT                                                                         <  SRR4302534.5095567/94‑1 (MQ=60)

                      GTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTC                                                                        >  SRR4302534.1347540/1‑94 (MQ=60)

                       TGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCC                                                                       >  SRR4302534.4161369/1‑94 (MQ=60)

                          GTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCA                                                                    >  SRR4302534.2126885/1‑94 (MQ=60)

                          GTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCA                                                                    <  SRR4302534.4490879/94‑1 (MQ=60)

                               AACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCAT                                                               >  SRR4302534.1379766/1‑94 (MQ=60)

                               AACGCATTGATCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTCGTCGATTCCGCAGTCAT                                                               >  SRR4302534.6477000/1‑94 (MQ=60)

                                ACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCTGCGTGATTTAGTCGATTCCGCAGTCATT                                                              <  SRR4302534.5262179/94‑1 (MQ=60)

                                 CGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCCTTG                                                             >  SRR4302534.6617588/1‑94 (MQ=60)

                                 CGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTG                                                             <  SRR4302534.4688887/94‑1 (MQ=60)

                                  GCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGT                                                            >  SRR4302534.689814/1‑94 (MQ=60)

                                   CATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTA                                                           <  SRR4302534.3173087/94‑1 (MQ=60)

                                   CATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTA                                                           >  SRR4302534.603486/1‑94 (MQ=60)

                                   CATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTA                                                           >  SRR4302534.6124101/1‑94 (MQ=60)

                                      TGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAG                                                        >  SRR4302534.3770703/1‑94 (MQ=60)

                                       GGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGT                                                       <  SRR4302534.4706709/94‑1 (MQ=60)

                                         TCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGT                                                     <  SRR4302534.3625536/94‑1 (MQ=60)

                                           GCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTAT                                                   <  SRR4302534.6407331/94‑1 (MQ=60)

                                           GCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTAT                                                   <  SRR4302534.1500970/94‑1 (MQ=60)

                                           GCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTAT                                                   <  SRR4302534.204071/94‑1 (MQ=60)

                                             GCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGT                                                 >  SRR4302534.6249243/1‑94 (MQ=60)

                                             GCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGT                                                 >  SRR4302534.199199/1‑94 (MQ=60)

                                             GCCGGACATCAGGTTGGCGGTGTATCGAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGT                                                 <  SRR4302534.6321087/94‑1 (MQ=60)

                                              CCGGACATCAGGTTGGCTGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTT                                                <  SRR4302534.3796910/94‑1 (MQ=60)

                                               CGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTC                                               <  SRR4302534.121937/94‑1 (MQ=60)

                                                  ACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCA                                            >  SRR4302534.5274857/1‑94 (MQ=60)

                                                   CATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAG                                           >  SRR4302534.975329/1‑94 (MQ=60)

                                                       AGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAG                                       >  SRR4302534.1661628/1‑94 (MQ=60)

                                                       AGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAG                                       <  SRR4302534.5467659/94‑1 (MQ=60)

                                                       AGGTTGGCGGTGTATCTAAAGGGCTGTTTCTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAG                                       <  SRR4302534.6523296/94‑1 (MQ=60)

                                                        GGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGG                                      <  SRR4302534.1313695/94‑1 (MQ=60)

                                                         GTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGC                                     <  SRR4302534.4991014/94‑1 (MQ=60)

                                                          TTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCT                                    >  SRR4302534.4931232/1‑94 (MQ=60)

                                                          TTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCT                                    <  SRR4302534.2743155/94‑1 (MQ=60)

                                                          TTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTCAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCT                                    <  SRR4302534.1812788/94‑1 (MQ=60)

                                                           TGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTG                                   <  SRR4302534.4161791/94‑1 (MQ=60)

                                                           TGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTG                                   <  SRR4302534.5883583/94‑1 (MQ=60)

                                                             GCGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGA                                 >  SRR4302534.2722268/1‑94 (MQ=60)

                                                              CGGTGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGGGAATGTTCGCAGGCAGGCTGGAA                                >  SRR4302534.2733902/1‑94 (MQ=60)

                                                                 TGTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAAC                             >  SRR4302534.3299014/1‑94 (MQ=60)

                                                                  GTATCTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACC                            <  SRR4302534.581202/94‑1 (MQ=60)

                                                                   TATCTAAAGGGCTGTTTTTCCCCCGCACGCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCT                           <  SRR4302534.2217052/94‑1 (MQ=60)

                                                                      CTAAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGAC                        <  SRR4302534.154110/94‑1 (MQ=60)

                                                                        AAAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACAC                      <  SRR4302534.5103825/94‑1 (MQ=60)

                                                                         AAGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGCCACC                     >  SRR4302534.1514704/1‑94 (MQ=60)

                                                                          AGGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCA                    >  SRR4302534.5855250/1‑94 (MQ=60)

                                                                           GGGCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCAC                   >  SRR4302534.4372525/1‑94 (MQ=60)

                                                                             GCTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCG                 <  SRR4302534.377073/94‑1 (MQ=60)

                                                                              CTGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGC                >  SRR4302534.3934774/1‑94 (MQ=60)

                                                                               TGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAAACTGACACCACCGCA               >  SRR4302534.3576491/1‑94 (MQ=60)

                                                                               TGTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGGGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCA               >  SRR4302534.2993263/1‑94 (MQ=60)

                                                                                GTTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCAACGCAA              >  SRR4302534.2554537/1‑94 (MQ=60)

                                                                                 TTTTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATA             <  SRR4302534.1220719/94‑1 (MQ=60)

                                                                                   TTTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAAT           >  SRR4302534.3080944/1‑94 (MQ=60)

                                                                                    TTCCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATC          <  SRR4302534.4081028/94‑1 (MQ=60)

                                                                                      CCCCCGCGCCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCT        <  SRR4302534.4056305/94‑1 (MQ=60)

                                                                                      CCCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCT        >  SRR4302534.5657176/1‑94 (MQ=60)

                                                                                       CCCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTC       >  SRR4302534.3559455/1‑94 (MQ=60)

                                                                                        CCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCT      >  SRR4302534.5136332/1‑94 (MQ=60)

                                                                                        CCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCT      <  SRR4302534.3217848/94‑1 (MQ=60)

                                                                                        CCCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCT      <  SRR4302534.982265/94‑1 (MQ=60)

                                                                                         CCGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTC     <  SRR4302534.2107470/94‑1 (MQ=60)

                                                                                          CGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCG    <  SRR4302534.6099719/94‑1 (MQ=60)

                                                                                          CGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCG    <  SRR4302534.4304433/94‑1 (MQ=60)

                                                                                          CGCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCG    <  SRR4302534.3658834/94‑1 (MQ=60)

                                                                                           GCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGA   >  SRR4302534.5111346/1‑94 (MQ=60)

                                                                                           GCACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTCACACCACCGCATAATCCTCTCGA   >  SRR4302534.6234397/1‑94 (MQ=60)

                                                                                            CACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCCAC  >  SRR4302534.1775990/1‑94 (MQ=60)

GCAACGCCGCTTTCTGTTGCCAGTGGGTTACAACGCATTGGTCGCGCCGGACATCAGGTTGGCGGTGTATCTAAAGGGCTGTTTTTCCCCCGTACCCGGCGTGATTTAGTCGATTCCGCAGTCATTGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGAC  >  NC_000913/1728311‑1728496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`