Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,730,968 T→C S1286S (AGT→AGC)  lhr → Lhr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,730,9680TC98.6% 703.3 / ‑2.2 212S1286S (AGT→AGC) lhrLhr
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (116/92);  total (119/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 224 total aligned reads displayed.

TGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTT  >  NC_000913/1730878‑1731056
                                                                                          |                                                                                        
tGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTc                                                                                        >  1:4149083/1‑93 (MQ=255)
 gCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTcc                                                                                       >  1:3054196/1‑93 (MQ=255)
   ccTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCaa                                                                                     >  1:5594039/1‑93 (MQ=255)
   ccTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCaa                                                                                     >  2:6147082/1‑93 (MQ=255)
   ccTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCaa                                                                                     <  1:6027837/93‑1 (MQ=255)
   ccTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCaa                                                                                     <  2:2652174/93‑1 (MQ=255)
   ccTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCaa                                                                                     >  2:1662247/1‑93 (MQ=255)
    cTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAac                                                                                    >  2:3914550/1‑93 (MQ=255)
    cTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAac                                                                                    <  1:687064/93‑1 (MQ=255)
    cTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAac                                                                                    <  1:8128622/93‑1 (MQ=255)
     tGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAacg                                                                                   >  1:8398962/1‑93 (MQ=255)
        aaGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAacgcac                                                                                <  1:6634975/93‑1 (MQ=255)
        aaGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAacgcac                                                                                <  1:8020802/93‑1 (MQ=255)
         agagGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAacgcacg                                                                               <  2:7372275/93‑1 (MQ=255)
         agagGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAacgcacg                                                                               >  1:2419687/1‑93 (MQ=255)
          gagGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAacgcacgc                                                                              >  1:2616227/1‑93 (MQ=255)
          gagGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAacgcacgc                                                                              >  2:3126079/1‑93 (MQ=255)
          gagGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAacgcacgc                                                                              >  2:4910382/1‑93 (MQ=255)
            ggCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACgcacgcac                                                                            >  1:8759781/1‑93 (MQ=255)
             gCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCAcc                                                                           <  2:2668118/93‑1 (MQ=255)
               gTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTc                                                                         >  1:6498681/1‑93 (MQ=255)
               gTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTc                                                                         >  1:1240090/1‑93 (MQ=255)
                 tATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCaa                                                                       >  1:5380916/1‑93 (MQ=255)
                 tATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCaa                                                                       >  2:3247227/1‑93 (MQ=255)
                  ttGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAAc                                                                      <  2:6727322/92‑1 (MQ=255)
                  aTGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAAc                                                                      <  1:3580037/93‑1 (MQ=255)
                    gggCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTc                                                                    <  2:4058369/93‑1 (MQ=255)
                    gggCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTc                                                                    <  2:6923799/93‑1 (MQ=255)
                     ggCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTcg                                                                   <  1:2261791/93‑1 (MQ=255)
                       cgcTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTcgcc                                                                 >  2:7207105/1‑93 (MQ=255)
                       cgcTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTcgcc                                                                 <  2:3953577/93‑1 (MQ=255)
                         cTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCACCTcgccgc                                                               >  1:7222687/1‑93 (MQ=255)
                         cTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTcgccgc                                                               <  2:1811143/93‑1 (MQ=255)
                           ggTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAg                                                             >  2:1955870/1‑93 (MQ=255)
                            gTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGt                                                            <  1:4210299/93‑1 (MQ=255)
                            gTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGt                                                            <  1:2099602/93‑1 (MQ=255)
                            gTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGt                                                            <  1:470341/93‑1 (MQ=255)
                            gTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGt                                                            >  2:5473289/1‑93 (MQ=255)
                               tGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTGCGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCAc                                                         <  2:6988290/93‑1 (MQ=255)
                                ggCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCCACGCACGCACCTCAACTCGCCGCAGTCAcc                                                        >  1:7313106/1‑93 (MQ=255)
                                 gCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCg                                                       >  2:3754209/1‑93 (MQ=255)
                                   aaGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCggg                                                     >  2:4190088/1‑93 (MQ=255)
                                   aaGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCggg                                                     >  2:4448773/1‑93 (MQ=255)
                                   aaGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCggg                                                     >  1:1526995/1‑93 (MQ=255)
                                   aaGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCggg                                                     >  1:5126875/1‑93 (MQ=255)
                                   aaGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCggg                                                     >  2:7075669/1‑93 (MQ=255)
                                    aGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGc                                                    >  1:1726462/1‑93 (MQ=255)
                                     ggCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCt                                                   >  2:2060170/1‑93 (MQ=255)
                                     ggCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCt                                                   <  1:4597657/93‑1 (MQ=255)
                                     ggCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCt                                                   <  1:4924807/93‑1 (MQ=255)
                                        gTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCtcgt                                                >  1:3841462/1‑93 (MQ=255)
                                        gTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCtcgt                                                >  2:5560413/1‑93 (MQ=255)
                                         tcatcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCtcgtc                                               <  2:3348889/93‑1 (MQ=255)
                                         tcatcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCtcgtc                                               >  1:4061232/1‑93 (MQ=255)
                                         tcatcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCtcgtc                                               >  2:4604373/1‑93 (MQ=255)
                                         tcatcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCtcgtc                                               >  2:4738076/1‑93 (MQ=255)
                                           atcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCCGTCATCGCGCtcgtcgg                                             >  2:5977237/1‑92 (MQ=255)
                                           atcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTcgtcgt                                             >  1:5767086/1‑93 (MQ=255)
                                            tcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTg                                            <  1:4783110/93‑1 (MQ=255)
                                            tcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTg                                            <  2:1478978/93‑1 (MQ=255)
                                            tcaCCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTg                                            >  1:3528812/1‑93 (MQ=255)
                                              cccAGCGACATTTGGGCCCCGTTACGCGCCCTCCCCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGa                                          <  2:5813164/92‑1 (MQ=255)
                                               ccAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGAc                                         >  1:1937233/1‑93 (MQ=255)
                                                 aGCGACATTTTGGCACCGTTACGCGCCCTCGCCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGt                                       <  1:52533/93‑1 (MQ=255)
                                                 aGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGt                                       <  2:4346005/93‑1 (MQ=255)
                                                 aGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGt                                       >  2:3567758/1‑93 (MQ=255)
                                                  gCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCATGGACggc                                      >  2:7432580/1‑91 (MQ=255)
                                                  acGACATTGAGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTc                                      <  2:2277245/92‑1 (MQ=255)
                                                   cGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTcc                                     <  1:7077496/93‑1 (MQ=255)
                                                    gACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCt                                    <  1:8351497/93‑1 (MQ=255)
                                                    gACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCt                                    >  2:8812536/1‑93 (MQ=255)
                                                    gACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCt                                    >  2:1404715/1‑93 (MQ=255)
                                                       aTTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGtt                                 >  1:2876117/1‑92 (MQ=255)
                                                        tttGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCt                                >  1:5104743/1‑93 (MQ=255)
                                                          tGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTAt                              >  2:6114662/1‑93 (MQ=255)
                                                            ggCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATgc                            <  1:6300510/93‑1 (MQ=255)
                                                            ggCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATgc                            <  2:2450851/93‑1 (MQ=255)
                                                             gCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATgcg                           >  1:8500474/1‑93 (MQ=255)
                                                               aCCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCa                         >  1:1293066/1‑93 (MQ=255)
                                                               aCCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCa                         >  2:759216/1‑93 (MQ=255)
                                                               aCCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCa                         >  2:80002/1‑93 (MQ=255)
                                                               aCCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCa                         <  2:8244814/93‑1 (MQ=255)
                                                                 cGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAAc                       >  1:2173448/1‑93 (MQ=255)
                                                                 cGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAAc                       <  1:7463741/93‑1 (MQ=255)
                                                                  gTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAAcc                      >  1:3367690/1‑93 (MQ=255)
                                                                  gTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAAcc                      >  1:2580245/1‑93 (MQ=255)
                                                                     aCGCGCCCTCACCCGCAGCAGTTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGGGGACGTCCTGTCTATGCGCAACCCgc                   >  1:6077978/1‑92 (MQ=255)
                                                                     aCGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGt                   <  2:1301427/93‑1 (MQ=255)
                                                                      cgcgCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGTACGTCCTGTCTATGCGCAACCCGtc                  >  2:510102/1‑93 (MQ=255)
                                                                       gcgcCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGtct                 >  2:7568648/1‑93 (MQ=255)
                                                                       ccgcCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGtct                 <  1:1022556/92‑1 (MQ=255)
                                                                         gcCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTcg               >  1:6946389/1‑93 (MQ=255)
                                                                          cccTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTcgc              >  2:3778521/1‑93 (MQ=255)
                                                                          cccTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTcgc              >  2:5722317/1‑93 (MQ=255)
                                                                          cccTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTcgc              <  1:5984556/93‑1 (MQ=255)
                                                                            cTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTcgccg            <  1:6755769/93‑1 (MQ=255)
                                                                             tCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTcgccgc           >  1:8236455/1‑93 (MQ=255)
                                                                                cccGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCggg        <  2:410571/93‑1 (MQ=255)
                                                                                   gcagcaGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTAt     >  2:1248297/1‑93 (MQ=255)
                                                                                      gcagCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCtt  >  2:89865/1‑93 (MQ=255)
                                                                                          |                                                                                        
TGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTT  >  NC_000913/1730878‑1731056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACAAC  >  NC_000913/1730875‑1731061
                                                                                             |                                                                                             
CTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGC                                                                                               >  SRR4302541.2406083/1‑94 (MQ=60)
CTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGC                                                                                               >  SRR4302541.2406084/1‑94 (MQ=60)
CTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGCCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGC                                                                                               >  SRR4302541.1513928/1‑94 (MQ=60)
 TCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCT                                                                                              <  SRR4302541.5992891/94‑1 (MQ=60)
 TCTGCCCTGCAAGAGGCGTTAGGGGCGCGGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCCCCCGCAGCAGCT                                                                                              <  SRR4302541.5992890/94‑1 (MQ=60)
  CTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTC                                                                                             >  SRR4302541.4190102/1‑94 (MQ=60)
  CTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTC                                                                                             >  SRR4302541.4190103/1‑94 (MQ=60)
  CTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTC                                                                                             >  SRR4302541.1767201/1‑94 (MQ=60)
   TGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCC                                                                                            >  SRR4302541.3085122/1‑94 (MQ=60)
     CCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAA                                                                                          >  SRR4302541.5651294/1‑94 (MQ=60)
     CCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAA                                                                                          <  SRR4302541.2201738/94‑1 (MQ=60)
     CCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAA                                                                                          <  SRR4302541.4045097/94‑1 (MQ=60)
      CCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAAC                                                                                         <  SRR4302541.6089591/94‑1 (MQ=60)
       CTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACG                                                                                        <  SRR4302541.693534/94‑1 (MQ=60)
       CTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACG                                                                                        <  SRR4302541.8217609/94‑1 (MQ=60)
       CTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACG                                                                                        >  SRR4302541.8489845/1‑94 (MQ=60)
       CTGCAAGACGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACG                                                                                        <  SRR4302541.4695163/94‑1 (MQ=60)
          CAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCAC                                                                                     <  SRR4302541.1612979/94‑1 (MQ=60)
          CAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCAC                                                                                     <  SRR4302541.5811710/94‑1 (MQ=60)
           AAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACG                                                                                    >  SRR4302541.2443552/1‑94 (MQ=60)
           AAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACG                                                                                    <  SRR4302541.8108984/94‑1 (MQ=60)
           AAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACG                                                                                    <  SRR4302541.6702996/94‑1 (MQ=60)
            AGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGC                                                                                   >  SRR4302541.2642360/1‑94 (MQ=60)
              AGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACGCACGCAC                                                                                 >  SRR4302541.8853476/1‑94 (MQ=60)
               GGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACC                                                                                <  SRR4302541.5337852/94‑1 (MQ=60)
                 CGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTC                                                                              >  SRR4302541.1252335/1‑94 (MQ=60)
                 CGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTC                                                                              >  SRR4302541.6564951/1‑94 (MQ=60)
                  GTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCA                                                                             >  SRR4302541.819594/1‑94 (MQ=60)
                   TTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCCCCCCCAGCAGCTCCAACGCACGCCCCTCAC                                                                            >  SRR4302541.2823780/1‑94 (MQ=60)
                   TTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAA                                                                            >  SRR4302541.5436144/1‑94 (MQ=60)
                   TTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAA                                                                            >  SRR4302541.2102045/1‑94 (MQ=60)
                     ATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACT                                                                          <  SRR4302541.2617868/94‑1 (MQ=60)
                     ATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACT                                                                          <  SRR4302541.6146650/94‑1 (MQ=60)
                     ATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACT                                                                          <  SRR4302541.3616317/94‑1 (MQ=60)
                      TGGGCGCTGGTCTCGCGAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCGCCTCAACTC                                                                         <  SRR4302541.2948768/94‑1 (MQ=60)
                       GGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACACTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCCCCAACGCACGCACCTCAACTCC                                                                        >  SRR4302541.5135908/1‑94 (MQ=60)
                        GGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGC                                                                       <  SRR4302541.2283630/94‑1 (MQ=60)
                         GCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCC                                                                      <  SRR4302541.5013460/94‑1 (MQ=60)
                           GCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCACCTCGCCGC                                                                    >  SRR4302541.7299743/1‑94 (MQ=60)
                             TGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAG                                                                  >  SRR4302541.6101748/1‑94 (MQ=60)
                               GTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTC                                                                <  SRR4302541.2119945/94‑1 (MQ=60)
                               GTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTC                                                                <  SRR4302541.474813/94‑1 (MQ=60)
                               GTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTC                                                                <  SRR4302541.4252176/94‑1 (MQ=60)
                               GTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCCCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTC                                                                <  SRR4302541.4252178/94‑1 (MQ=60)
                                TCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCA                                                               <  SRR4302541.3604487/94‑1 (MQ=60)
                                TCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCA                                                               >  SRR4302541.7541757/1‑94 (MQ=60)
                                 CTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACGCACGCACCTCAACTCGCCGCAGTCAC                                                              <  SRR4302541.2435396/94‑1 (MQ=60)
                                  TGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCCACGCACGCACCTCAACTCGCCGCAGTCACC                                                             >  SRR4302541.7391173/1‑94 (MQ=60)
                                   GGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCG                                                            >  SRR4302541.7345151/1‑94 (MQ=60)
                                   GGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCG                                                            <  SRR4302541.8575202/94‑1 (MQ=60)
                                    GCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGG                                                           <  SRR4302541.3007451/94‑1 (MQ=60)
                                     CAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGG                                                          >  SRR4302541.1542262/1‑94 (MQ=60)
                                     CAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGG                                                          >  SRR4302541.5179575/1‑94 (MQ=60)
                                     CAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGG                                                          >  SRR4302541.5678073/1‑94 (MQ=60)
                                     CAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGC                                                          >  SRR4302541.5179574/1‑94 (MQ=60)
                                      AAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGC                                                         >  SRR4302541.6917738/1‑94 (MQ=60)
                                      AAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGC                                                         >  SRR4302541.1743325/1‑94 (MQ=60)
                                       AGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCT                                                        >  SRR4302541.480284/1‑94 (MQ=60)
                                        GGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTC                                                       <  SRR4302541.4644312/94‑1 (MQ=60)
                                        GGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTC                                                       >  SRR4302541.6595806/1‑94 (MQ=60)
                                        GGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTC                                                       <  SRR4302541.3166637/94‑1 (MQ=60)
                                        GGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTC                                                       <  SRR4302541.4975051/94‑1 (MQ=60)
                                        GGCGTCACCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTC                                                       <  SRR4302541.7641180/94‑1 (MQ=60)
                                          CGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGT                                                     >  SRR4302541.3880152/1‑94 (MQ=60)
                                          CGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGT                                                     >  SRR4302541.2120208/1‑94 (MQ=60)
                                           GTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTC                                                    >  SRR4302541.4101308/1‑94 (MQ=60)
                                           GTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTC                                                    <  SRR4302541.7242872/94‑1 (MQ=60)
                                           GTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGATCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTC                                                    >  SRR4302541.4964833/1‑94 (MQ=60)
                                             CATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGT                                                  >  SRR4302541.5825777/1‑94 (MQ=60)
                                              ATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTG                                                 >  SRR4302541.6474980/1‑94 (MQ=60)
                                              ATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTG                                                 >  SRR4302541.3564341/1‑94 (MQ=60)
                                               TCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGG                                                <  SRR4302541.4831929/94‑1 (MQ=60)
                                                CACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGA                                               <  SRR4302541.4411048/94‑1 (MQ=60)
                                                 CCCAGCGACATTTGGGCACCGTTACGCGCCCTCGCCCTCAGCAGCACTAACGCACGCACCTCAACGCGCCGCAGTCACCGGGCTCGTCGTGGAC                                              <  SRR4302541.1391444/94‑1 (MQ=60)
                                                 ACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGAC                                              >  SRR4302541.1955892/1‑94 (MQ=60)
                                                  CCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACG                                             >  SRR4302541.7311215/1‑94 (MQ=60)
                                                   CAGCGACATTTGGGCCCCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGT                                            <  SRR4302541.6077869/94‑1 (MQ=60)
                                                    AGCGACATTTTGGCACCGTTACGCGCCCTCGCCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTC                                           <  SRR4302541.52944/94‑1 (MQ=60)
                                                      CGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCT                                         >  SRR4302541.1379269/1‑94 (MQ=60)
                                                      CGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCT                                         <  SRR4302541.7152774/94‑1 (MQ=60)
                                                       GACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTG                                        <  SRR4302541.8442129/94‑1 (MQ=60)
                                                         CATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCACCTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTC                                      <  SRR4302541.8456845/94‑1 (MQ=60)
                                                         CATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTT                                      >  SRR4302541.2904976/1‑94 (MQ=60)
                                                          ATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCT                                     >  SRR4302541.5157186/1‑94 (MQ=60)
                                                           TTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCCCCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTA                                    <  SRR4302541.6708766/94‑1 (MQ=60)
                                                            TTGGGCACCGTTGCGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTAT                                   <  SRR4302541.3729923/94‑1 (MQ=60)
                                                               GGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCG                                >  SRR4302541.8592016/1‑94 (MQ=60)
                                                               GGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCG                                <  SRR4302541.7074017/94‑1 (MQ=60)
                                                               GGCACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCG                                <  SRR4302541.6364908/94‑1 (MQ=60)
                                                                 CACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCA                              >  SRR4302541.1305830/1‑94 (MQ=60)
                                                                 CACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCA                              <  SRR4302541.8230442/94‑1 (MQ=60)
                                                                 CACCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCA                              >  SRR4302541.2319326/1‑94 (MQ=60)
                                                                   CCGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAAC                            >  SRR4302541.2194456/1‑94 (MQ=60)
                                                                    CGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACC                           <  SRR4302541.7543499/94‑1 (MQ=60)
                                                                    CGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACC                           >  SRR4302541.3401282/1‑94 (MQ=60)
                                                                    CGTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACC                           >  SRR4302541.2606111/1‑94 (MQ=60)
                                                                     GTTACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCC                          >  SRR4302541.3474303/1‑94 (MQ=60)
                                                                       TACGCGCCCTCACCCGCAGCAGTTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGGGGACGTCCTGTCTATGCGCAACCCGC                        >  SRR4302541.6140026/1‑94 (MQ=60)
                                                                       TACGCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGT                        >  SRR4302541.8597533/1‑94 (MQ=60)
                                                                         CGCGCCCACACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCT                      <  SRR4302541.370284/94‑1 (MQ=60)
                                                                          GCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTC                     >  SRR4302541.5927069/1‑94 (MQ=60)
                                                                          CCGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTC                     <  SRR4302541.1032004/94‑1 (MQ=60)
                                                                           CGCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCG                    >  SRR4302541.7020476/1‑94 (MQ=60)
                                                                            GCCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGC                   >  SRR4302541.415741/1‑94 (MQ=60)
                                                                             CCCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCC                  <  SRR4302541.6045810/94‑1 (MQ=60)
                                                                              CCTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGCCTCGCCG                 >  SRR4302541.1172953/1‑94 (MQ=60)
                                                                               CTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGC                >  SRR4302541.182053/1‑94 (MQ=60)
                                                                               CTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGC                >  SRR4302541.8326230/1‑94 (MQ=60)
                                                                               CTCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGC                <  SRR4302541.6828019/94‑1 (MQ=60)
                                                                                TCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCG               >  SRR4302541.5496553/1‑94 (MQ=60)
                                                                                TCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCG               <  SRR4302541.1273018/94‑1 (MQ=60)
                                                                                TCACCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCG               >  SRR4302541.6352076/1‑94 (MQ=60)
                                                                                  CCCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGG             <  SRR4302541.8655575/94‑1 (MQ=60)
                                                                                   CCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGT            >  SRR4302541.2543526/1‑94 (MQ=60)
                                                                                   CCCGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGG            >  SRR4302541.5238441/1‑94 (MQ=60)
                                                                                     CGCAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTAT          >  SRR4302541.6953043/1‑94 (MQ=60)
                                                                                       CAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCT        <  SRR4302541.8248222/94‑1 (MQ=60)
                                                                                       CAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCT        >  SRR4302541.8333134/1‑94 (MQ=60)
                                                                                       CAGCAGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCT        >  SRR4302541.6943856/1‑94 (MQ=60)
                                                                                           AGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACA    <  SRR4302541.4783159/94‑1 (MQ=60)
                                                                                           AGCTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACA    <  SRR4302541.4783160/94‑1 (MQ=60)
                                                                                             CTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACAAC  <  SRR4302541.2243751/94‑1 (MQ=60)
                                                                                             CTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACAAC  >  SRR4302541.2463792/1‑94 (MQ=60)
                                                                                             CTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACAAC  >  SRR4302541.3556964/1‑94 (MQ=60)
                                                                                             CTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACAAC  >  SRR4302541.4525884/1‑94 (MQ=60)
                                                                                             CTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACAAC  >  SRR4302541.4691652/1‑94 (MQ=60)
                                                                                             CTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACAAC  >  SRR4302541.4691659/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTCTGCCCTGCAAGAGGCGTTATGGGCGCTGGTCTGGCAAGGCGTCATCACCAGCGACATTTGGGCACCGTTACGCGCCCTCACCCGCAGCAGTTCCAACGCACGCACCTCAACTCGCCGCAGTCACCGGGCTCGTCGTGGACGTCCTGTCTATGCGCAACCCGTCTCGCCGCGGGTATCTTACAAC  >  NC_000913/1730875‑1731061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑