Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,741,574 2 bp→GA coding (94‑95/1374 nt) ydhE → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,741,5740AG100.0% 563.1 / NA 161S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (82/79);  total (82/79)
*NC_0009131,741,5750GA99.4% 613.0 / NA 161S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (81/79);  total (82/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741487‑1741661
                                                                                       ||                                                                                      
aaGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAcc                                                                                    >  2:1554110/1‑93 (MQ=255)
 aGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCg                                                                                   >  1:3439125/1‑93 (MQ=255)
  gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                  <  1:3862476/93‑1 (MQ=255)
  gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                  >  2:2792765/1‑93 (MQ=255)
  gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                  <  1:2096482/93‑1 (MQ=255)
  gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                  >  2:5041317/1‑93 (MQ=255)
    atatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGa                                                                                >  2:1608254/1‑93 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  1:109973/93‑1 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  1:5150968/93‑1 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               >  2:3487310/1‑93 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               >  1:1650376/1‑93 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               >  2:4927878/1‑93 (MQ=255)
       tCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATgg                                                                             >  1:5020618/1‑93 (MQ=255)
       tCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATgg                                                                             >  2:3684213/1‑93 (MQ=255)
        cAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGTTGGGTTTTGTCGATACCGTGATggc                                                                            >  2:2975320/1‑93 (MQ=255)
        cAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggc                                                                            >  1:5372956/1‑93 (MQ=255)
           tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcggg                                                                         <  2:3954864/93‑1 (MQ=255)
           tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcggg                                                                         >  2:5897682/1‑93 (MQ=255)
            gAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggc                                                                        <  2:1194970/93‑1 (MQ=255)
             aaGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggcg                                                                       <  1:3776429/93‑1 (MQ=255)
               gcgcGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGggcggc                                                                     <  1:2729192/93‑1 (MQ=255)
               gcgcGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGggcggc                                                                     >  1:4375080/1‑93 (MQ=255)
                cgcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCt                                                                    >  1:4218745/1‑93 (MQ=255)
                 gcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGGTACCGTGATGGCGGGGGGCta                                                                   >  2:1157003/1‑93 (MQ=255)
                 gcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                   <  2:4544208/93‑1 (MQ=255)
                 gcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                   >  1:2505923/1‑93 (MQ=255)
                 gcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                   >  1:3541785/1‑93 (MQ=255)
                  cgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtat                                                                  >  1:3988330/1‑93 (MQ=255)
                  cgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtat                                                                  >  2:750832/1‑93 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                >  2:2495994/1‑93 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                >  1:3540051/1‑93 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                >  2:5445980/1‑93 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                >  2:4437334/1‑93 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              <  1:3701829/93‑1 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  2:120954/1‑93 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  1:5680309/1‑93 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGGTTTGTCGATACCGTGATGGCGGGCGGCCATAGTg                                                              >  1:6444275/1‑93 (MQ=255)
                        ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            >  2:4442556/1‑93 (MQ=255)
                        ttattaGCATGAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            <  1:1557426/93‑1 (MQ=255)
                           ttagcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAcc                                                         <  2:927867/93‑1 (MQ=255)
                             agcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                       <  2:2684317/93‑1 (MQ=255)
                               cattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACa                                                     <  1:5863750/93‑1 (MQ=255)
                                attagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    <  2:269972/93‑1 (MQ=255)
                                attagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    >  2:849916/1‑93 (MQ=255)
                                attagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    >  2:3210684/1‑93 (MQ=255)
                                  tagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                  <  1:2177170/93‑1 (MQ=255)
                                   agcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggc                                                 <  2:2453351/93‑1 (MQ=255)
                                    gcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcg                                                <  1:3111858/93‑1 (MQ=255)
                                     caATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCCCCGACATggcgg                                               >  2:4800008/1‑93 (MQ=255)
                                     caATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcgg                                               <  2:5554484/93‑1 (MQ=255)
                                     caATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcgg                                               <  1:3259871/93‑1 (MQ=255)
                                     caATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcgg                                               <  1:2261592/93‑1 (MQ=255)
                                      aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggg                                              >  1:1195033/1‑92 (MQ=255)
                                       aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             >  1:6414154/1‑93 (MQ=255)
                                       aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             >  2:5750062/1‑93 (MQ=255)
                                        tCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATTGTGCCAGCGACATCGcggcgg                                            >  2:4111770/1‑93 (MQ=255)
                                        tCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGGGCCACCGACATGGcggcgg                                            >  1:2603756/1‑93 (MQ=255)
                                         cccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGt                                           <  2:3368251/93‑1 (MQ=255)
                                          ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGcggcgggc                                          >  1:4360855/1‑91 (MQ=255)
                                          ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTc                                          <  2:4587568/93‑1 (MQ=255)
                                          ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTc                                          <  1:4769186/93‑1 (MQ=255)
                                               gATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAt                                     <  1:41415/93‑1 (MQ=255)
                                                     cgcgCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAc                               >  1:2977175/1‑93 (MQ=255)
                                                      gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACt                              <  2:1684599/93‑1 (MQ=255)
                                                      gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACt                              <  1:6049159/93‑1 (MQ=255)
                                                       cgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGGGGTCGCTATCGGTACtt                             >  2:3104218/1‑93 (MQ=255)
                                                        gcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGGCATGGCGGCGGTCGCTATCCGTACTTc                            >  1:2022799/1‑93 (MQ=255)
                                                        gcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTc                            <  2:5790040/93‑1 (MQ=255)
                                                           aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAt                         >  1:3377357/1‑93 (MQ=255)
                                                           aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAt                         >  2:346886/1‑93 (MQ=255)
                                                            aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        >  2:5446153/1‑93 (MQ=255)
                                                            aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        >  2:179171/1‑93 (MQ=255)
                                                             tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCt                       >  2:6397080/1‑93 (MQ=255)
                                                             tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCt                       >  2:2321061/1‑93 (MQ=255)
                                                              cGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                      <  2:5462033/93‑1 (MQ=255)
                                                              cGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                      <  1:4341832/93‑1 (MQ=255)
                                                               gCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCggg                     <  1:4974625/93‑1 (MQ=255)
                                                                cccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGc                    >  2:5984800/1‑93 (MQ=255)
                                                                  cAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCtt                  <  2:1809841/93‑1 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTTCg               >  1:4095354/1‑93 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               <  1:2445930/93‑1 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               >  1:1446105/1‑93 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               <  2:6220734/93‑1 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               >  2:3567152/1‑93 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTTTTTCTGGCTTctg               >  2:2344386/1‑91 (MQ=255)
                                                                     aCTACGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               <  2:6220733/93‑1 (MQ=255)
                                                                      cTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCgg              >  2:2250133/1‑93 (MQ=255)
                                                                          gATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTTCGGCGAt          >  2:3204785/1‑93 (MQ=255)
                                                                          gATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAt          <  1:2460902/93‑1 (MQ=255)
                                                                           aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAACGGTACTTCTATCTGGCTTCAGGGGATc         >  1:3067402/1‑93 (MQ=255)
                                                                            tGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAtac        >  1:1990615/1‑91 (MQ=255)
                                                                            tGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATcc        >  1:4614668/1‑93 (MQ=255)
                                                                            tGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATcc        >  2:5996658/1‑93 (MQ=255)
                                                                             gggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCct       <  2:5661623/93‑1 (MQ=255)
                                                                                ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    <  2:3040352/93‑1 (MQ=255)
                                                                                 tttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCttt   <  2:369763/93‑1 (MQ=255)
                                                                                 tttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCttt   <  2:3783349/93‑1 (MQ=255)
                                                                                  ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  2:4852397/93‑1 (MQ=255)
                                                                                  ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  1:4541039/93‑1 (MQ=255)
                                                                                  ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  1:5407678/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741487‑1741661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  NC_000913/1741481‑1741663
                                                                                             |                                                                                         
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCG                                                                                           <  SRR4302534.1607900/94‑1 (MQ=60)
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCG                                                                                           >  SRR4302534.2244897/1‑94 (MQ=60)
      AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCG                                                                                     >  SRR4302534.3531560/1‑94 (MQ=60)
        GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                   <  SRR4302534.3966182/94‑1 (MQ=60)
        GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                   <  SRR4302534.2151484/94‑1 (MQ=60)
          ATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAT                                                                                 >  SRR4302534.1693399/1‑94 (MQ=60)
           TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                <  SRR4302534.112905/94‑1 (MQ=60)
           TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                <  SRR4302534.3988585/94‑1 (MQ=60)
           TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                >  SRR4302534.4346140/1‑94 (MQ=60)
           TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                <  SRR4302534.5289922/94‑1 (MQ=60)
            ATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGG                                                                               >  SRR4302534.5156258/1‑94 (MQ=60)
             TCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGC                                                                              <  SRR4302534.2600060/94‑1 (MQ=60)
             TCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGC                                                                              >  SRR4302534.5517739/1‑94 (MQ=60)
              CAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCG                                                                             >  SRR4302534.5489764/1‑94 (MQ=60)
                GTGAAGCTCGTCTGTTATTAGCATTAGCAGGCCCGGTGATTCTCGCTCAAGTCGCCCAAACTGCGATCGGTTTTGTCGATACCGTGATGGCTGG                                                                           <  SRR4302534.1960654/94‑1 (MQ=57)
                   AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGG                                                                        <  SRR4302534.3877818/94‑1 (MQ=60)
                    AGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC                                                                       >  SRR4302534.5010041/1‑94 (MQ=60)
                    AGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC                                                                       >  SRR4302534.4492999/1‑94 (MQ=60)
                     GCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCT                                                                      >  SRR4302534.4332489/1‑94 (MQ=60)
                     GCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCT                                                                      <  SRR4302534.2801166/94‑1 (MQ=60)
                      CGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTA                                                                     >  SRR4302534.2572463/1‑94 (MQ=60)
                      CGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTA                                                                     >  SRR4302534.3637065/1‑94 (MQ=60)
                       GCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTAT                                                                    >  SRR4302534.4095036/1‑94 (MQ=60)
                         GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAG                                                                  >  SRR4302534.3635282/1‑94 (MQ=60)
                         GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACCGCGATGGATTTTGCCGATACCGTGATGCCGGGCGGCTATAG                                                                  <  SRR4302534.6121976/94‑1 (MQ=60)
                           CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                                >  SRR4302534.5834117/1‑94 (MQ=60)
                           CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGGTTTGTCGATACCGTGATGGCGGGCGGCCATAGTG                                                                >  SRR4302534.6617936/1‑94 (MQ=60)
                            TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGC                                                               >  SRR4302534.2959066/1‑94 (MQ=60)
                            TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGC                                                               <  SRR4302534.3801337/94‑1 (MQ=60)
                              TTATTAGCATGAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCA                                                             <  SRR4302534.1598198/94‑1 (MQ=60)
                               TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAC                                                            <  SRR4302534.3319855/94‑1 (MQ=60)
                               TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAC                                                            >  SRR4302534.4225409/1‑94 (MQ=60)
                               TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAC                                                            >  SRR4302534.5271546/1‑94 (MQ=60)
                               TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAC                                                            <  SRR4302534.3504659/94‑1 (MQ=60)
                                ATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACC                                                           <  SRR4302534.2287387/94‑1 (MQ=60)
                                 TTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCG                                                          <  SRR4302534.3177032/94‑1 (MQ=60)
                                     CATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAT                                                      <  SRR4302534.6022959/94‑1 (MQ=60)
                                        TAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGC                                                   <  SRR4302534.2234690/94‑1 (MQ=60)
                                         aaccccccggttttttccCGCGCAAACCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                                  <  SRR4302534.5210410/76‑1 (MQ=60)
                                          GCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGG                                                 <  SRR4302534.3193694/94‑1 (MQ=60)
                                           CAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGG                                                >  SRR4302534.1226471/1‑94 (MQ=60)
                                           CAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                                <  SRR4302534.2321566/94‑1 (MQ=60)
                                           CAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                                <  SRR4302534.3346676/94‑1 (MQ=60)
                                           CAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                                >  SRR4302534.4663870/1‑94 (MQ=60)
                                            AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                               >  SRR4302534.6587215/1‑94 (MQ=60)
                                             ATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGGGCCACCGACATGGCGGCGG                                              >  SRR4302534.2672652/1‑94 (MQ=60)
                                               CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGC                                            >  SRR4302534.4478489/1‑94 (MQ=60)
                                                CCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCG                                           <  SRR4302534.1278929/94‑1 (MQ=60)
                                                CCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCG                                           <  SRR4302534.4898671/94‑1 (MQ=60)
                                                  GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         >  SRR4302534.446174/1‑94 (MQ=60)
                                                     GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302534.42525/94‑1 (MQ=60)
                                                     GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      >  SRR4302534.4700791/1‑94 (MQ=60)
                                                     GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATC                                      <  SRR4302534.6481544/94‑1 (MQ=60)
                                                          TCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAC                                 >  SRR4302534.3055100/1‑94 (MQ=60)
                                                            GCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTT                               <  SRR4302534.6213604/94‑1 (MQ=60)
                                                             CGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGGCATGGCGGCGGTCGCTATCCGTACTTC                              >  SRR4302534.2075711/1‑94 (MQ=60)
                                                             CGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTC                              >  SRR4302534.1601039/1‑94 (MQ=60)
                                                               CAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTA                            <  SRR4302534.2342079/94‑1 (MQ=60)
                                                                AAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAT                           >  SRR4302534.3467886/1‑94 (MQ=60)
                                                                    CGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGG                       <  SRR4302534.4459129/94‑1 (MQ=60)
                                                                     GCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCGGGC                      <  SRR4302534.5109293/94‑1 (MQ=60)
                                                                       CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                    <  SRR4302534.831475/94‑1 (MQ=60)
                                                                       CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                    <  SRR4302534.3743609/94‑1 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTTCG                 >  SRR4302534.4205423/1‑94 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 >  SRR4302534.1484165/1‑94 (MQ=60)
                                                                           ACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGG                <  SRR4302534.2510935/94‑1 (MQ=60)
                                                                            CTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGC               <  SRR4302534.3935521/94‑1 (MQ=60)
                                                                             TGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCG              >  SRR4302534.2125722/1‑94 (MQ=60)
                                                                                GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATC           <  SRR4302534.2526283/94‑1 (MQ=60)
                                                                                GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATC           >  SRR4302534.2572405/1‑94 (MQ=60)
                                                                                GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAACGGTACTTCTATCTGGCTTCAGGGGATC           >  SRR4302534.3147954/1‑94 (MQ=60)
                                                                                 ATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCC          >  SRR4302534.4739895/1‑94 (MQ=60)
                                                                                 ATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATAC          >  SRR4302534.2042741/1‑94 (MQ=60)
                                                                                    GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCT       <  SRR4302534.5556681/94‑1 (MQ=60)
                                                                                    GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCT       >  SRR4302534.3614177/1‑94 (MQ=60)
                                                                                      TTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTT     >  SRR4302534.6009562/1‑94 (MQ=60)
                                                                                        TTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGG   <  SRR4302534.4664135/94‑1 (MQ=60)
                                                                                        TTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGG   <  SRR4302534.5553178/94‑1 (MQ=60)
                                                                                         TGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  <  SRR4302534.5452321/94‑1 (MQ=60)
                                                                                             |                                                                                         
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  NC_000913/1741481‑1741663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑