Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,741,574 2 bp→GA coding (94‑95/1374 nt) ydhE → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,741,5740AG99.6% 826.5 / NA 242S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (108/131);  total (109/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*NC_0009131,741,5750GA100.0% 870.4 / NA 243S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (109/134);  total (109/134)

Only 100 of 251 total aligned reads displayed.

AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741487‑1741661
                                                                                       ||                                                                                      
aaGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAcc                                                                                    >  1:8616355/1‑93 (MQ=255)
 aGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCg                                                                                   >  2:3657410/1‑93 (MQ=255)
  gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                  <  1:8485494/93‑1 (MQ=255)
   tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGGTTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTg                                                                                 <  1:7644242/93‑1 (MQ=255)
   tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTg                                                                                 <  1:4963034/93‑1 (MQ=255)
   tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCCATACCGTg                                                                                 >  1:933357/1‑93 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGag                                                                               >  2:6709315/1‑92 (MQ=255)
     tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  2:7663618/93‑1 (MQ=255)
           tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcggg                                                                         <  1:1470316/93‑1 (MQ=255)
           tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcggg                                                                         <  2:4928219/93‑1 (MQ=255)
            gAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggg                                                                        >  1:346015/1‑92 (MQ=255)
              aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGgcgggcgg                                                                      >  2:8462991/1‑93 (MQ=255)
                 gcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                   <  1:3595654/93‑1 (MQ=255)
                   gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 >  2:7226333/1‑93 (MQ=255)
                   gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 <  2:416796/93‑1 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGGGGCTATAg                                                                >  2:1776252/1‑93 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                <  1:5392024/93‑1 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                >  1:3583806/1‑93 (MQ=255)
                    tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                <  2:7549903/93‑1 (MQ=255)
                     cTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGt                                                               >  1:2090838/1‑93 (MQ=255)
                     cTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGt                                                               <  1:7488112/93‑1 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  1:4575250/1‑93 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              <  2:5633295/93‑1 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  2:6225597/1‑93 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  2:8101183/1‑93 (MQ=255)
                      tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATAGGGTTTGTCGATACCGTGGTGGCGGGCGGCtatattt                                                              >  1:5181701/1‑90 (MQ=255)
                        ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            <  2:3266633/93‑1 (MQ=255)
                        ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            <  2:3928095/93‑1 (MQ=255)
                        ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            >  2:2749599/1‑93 (MQ=255)
                         tattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCa                                                           <  1:7000005/93‑1 (MQ=255)
                         tattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCa                                                           <  2:254676/93‑1 (MQ=255)
                          attagcattagcAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAc                                                          >  1:5485683/1‑93 (MQ=255)
                           ttagcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAcc                                                         <  1:6713405/93‑1 (MQ=255)
                           ttagcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAcc                                                         >  1:5870632/1‑93 (MQ=255)
                           ttagcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAcc                                                         >  1:3875125/1‑93 (MQ=255)
                             agcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                       <  1:1697268/93‑1 (MQ=255)
                               cattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCTATACCGTGATGGCGGGCGGCTATAGTGCCACCGACa                                                     <  2:4302551/93‑1 (MQ=255)
                                    gcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcg                                                <  1:8157746/93‑1 (MQ=255)
                                     caATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcgg                                               <  2:7051770/93‑1 (MQ=255)
                                     caATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcgg                                               <  2:114396/93‑1 (MQ=255)
                                      aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggc                                              <  1:4214968/93‑1 (MQ=255)
                                         cccGGTGATTCTCGCGCAAATCGTCCAAACTGCGCTGGGTTTTGTCGATACCGTGATGGCGGGCGGCCTTAGTGCCCGCCGCATGGAGGCGGt                                           >  2:2731550/1‑93 (MQ=255)
                                         cccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGcggcggg                                           >  1:6401536/1‑92 (MQ=255)
                                         cccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGt                                           <  1:3344305/93‑1 (MQ=255)
                                         cccGGTGATTCTCGCGCAAAGCGCTCGATCTGCGAGTGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGt                                           <  1:8784137/93‑1 (MQ=255)
                                          ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTc                                          <  1:5762951/93‑1 (MQ=255)
                                          ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGACGGGTTTTGTCGATCCCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTc                                          <  1:5365421/93‑1 (MQ=255)
                                            ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCCCCGACATGGCGGCGGTCGc                                        >  2:2950606/1‑93 (MQ=255)
                                            ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGc                                        <  1:1029678/93‑1 (MQ=255)
                                            ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGc                                        <  1:4718257/93‑1 (MQ=255)
                                            ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGc                                        >  1:4017294/1‑93 (MQ=255)
                                             gTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCt                                       >  1:5513675/1‑93 (MQ=255)
                                                aTTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGGGCTACCGACATGGCGGCCGTCCCCATc                                    >  1:7908968/1‑93 (MQ=255)
                                                 ttCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTCTCg                                   >  1:6997188/1‑93 (MQ=255)
                                                 ttCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                   >  2:8186747/1‑93 (MQ=255)
                                                 ttCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                   >  2:3451588/1‑93 (MQ=255)
                                                 ttCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                   >  1:7595786/1‑93 (MQ=255)
                                                    tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTa                                <  2:4373972/93‑1 (MQ=255)
                                                    tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTa                                >  1:3060202/1‑93 (MQ=255)
                                                      gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACt                              >  2:4635959/1‑93 (MQ=255)
                                                      gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACt                              >  2:5847471/1‑93 (MQ=255)
                                                       cgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATCCCGTGATGGCGGGCGGCTAGAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACtt                             <  2:412126/93‑1 (MQ=255)
                                                       cgcAAATCGCCCAAACGTGGATGGGTTTTGTCGATACCGTGATGGAGGGCGACTATAGTGCCACCGACATGGCGGCGGTCGATCTCGTTACtt                             <  2:1824609/93‑1 (MQ=255)
                                                        gcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTc                            <  1:7989214/93‑1 (MQ=255)
                                                         cAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCt                           >  2:1503207/1‑93 (MQ=255)
                                                         cAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCt                           <  2:5284746/93‑1 (MQ=255)
                                                         cAAATCACCCAAACTGCAATGGGTTTAGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGACGCTATCGGTACTTCt                           <  2:7817889/93‑1 (MQ=255)
                                                          aaaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTa                          >  2:7298639/1‑93 (MQ=255)
                                                          aaaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTa                          <  2:6425782/93‑1 (MQ=255)
                                                          aaaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTa                          >  1:1671078/1‑93 (MQ=255)
                                                          aaaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGGACTTCtc                          >  2:1939522/1‑92 (MQ=255)
                                                           aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAt                         >  2:3794049/1‑93 (MQ=255)
                                                            aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        <  1:667932/93‑1 (MQ=255)
                                                            aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        <  2:580998/93‑1 (MQ=255)
                                                             tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCGCCGACATGGCGGCGGTCGCTATCGGTACTTCTATCt                       <  1:7177596/93‑1 (MQ=255)
                                                             tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGCACTTCTATCt                       >  2:212283/1‑93 (MQ=255)
                                                              cGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                      >  2:5760090/1‑93 (MQ=255)
                                                               gCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTgg                     <  1:2218370/93‑1 (MQ=255)
                                                                  cAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCtt                  >  1:1440126/1‑93 (MQ=255)
                                                                   aaaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTc                 <  1:3638256/93‑1 (MQ=255)
                                                                    aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATCGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTca                <  2:7845025/93‑2 (MQ=255)
                                                                    aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTcc                <  2:4677326/93‑1 (MQ=255)
                                                                    aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTcc                <  1:776731/93‑1 (MQ=255)
                                                                    aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTcc                <  1:6319378/93‑1 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               >  2:4896650/1‑93 (MQ=255)
                                                                     aCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg               >  1:5267014/1‑93 (MQ=255)
                                                                      cTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCgg              <  1:3216773/93‑1 (MQ=255)
                                                                       cgCGATGTGTTTTGTCGATACCGTAGTGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCTGTACTTCTATCTGGCTTCCGGc             <  2:5216816/92‑1 (MQ=255)
                                                                         cGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATTGGTACTTCTATCTGGCTTCCGGCGa           <  2:2609599/93‑1 (MQ=255)
                                                                          gATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAt          >  2:6021244/1‑93 (MQ=255)
                                                                              ggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctc      >  1:42770/1‑93 (MQ=255)
                                                                               gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctct     >  1:2389458/1‑93 (MQ=255)
                                                                                ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    <  1:7531719/93‑1 (MQ=255)
                                                                                ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    <  1:2546305/93‑1 (MQ=255)
                                                                                ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    <  1:1267949/93‑1 (MQ=255)
                                                                                ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    >  1:5070988/1‑93 (MQ=255)
                                                                                 tttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCttt   <  2:1589203/93‑1 (MQ=255)
                                                                                 tttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCttt   <  2:6005535/93‑1 (MQ=255)
                                                                                  ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  2:1267162/93‑1 (MQ=255)
                                                                                  ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  1:2865287/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741487‑1741661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  NC_000913/1741481‑1741663
                                                                                             |                                                                                         
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCG                                                                                           >  SRR4302541.394959/1‑94 (MQ=60)
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCG                                                                                           <  SRR4302541.3969566/94‑1 (MQ=60)
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCG                                                                                           <  SRR4302541.3969567/94‑1 (MQ=60)
     GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                      >  SRR4302541.8708741/1‑94 (MQ=60)
     GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                      <  SRR4302541.4850338/94‑1 (MQ=60)
      AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCG                                                                                     <  SRR4302541.13258/94‑1 (MQ=60)
      AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCG                                                                                     <  SRR4302541.1461949/94‑1 (MQ=60)
       AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTTCGATGGGTTTTGTCGATACCGT                                                                                    <  SRR4302541.4025514/94‑1 (MQ=60)
        GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                   >  SRR4302541.1213577/1‑94 (MQ=60)
        GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                   <  SRR4302541.8576944/94‑1 (MQ=60)
        GTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCCATACCGTG                                                                                   >  SRR4302541.942182/1‑94 (MQ=60)
         TATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGGTTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGA                                                                                  <  SRR4302541.7725726/94‑1 (MQ=60)
         TATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGA                                                                                  <  SRR4302541.5013849/94‑1 (MQ=60)
          ATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAT                                                                                 >  SRR4302541.7114760/1‑94 (MQ=60)
           TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                >  SRR4302541.4239075/1‑94 (MQ=60)
           TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                <  SRR4302541.7679456/94‑1 (MQ=60)
            ATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGG‑TTTGTCGATACCGTGATGGC                                                                              >  SRR4302541.159478/1‑94 (MQ=60)
             TCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGC                                                                              <  SRR4302541.582859/94‑1 (MQ=60)
              CAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCG                                                                             >  SRR4302541.6013364/1‑94 (MQ=60)
              CAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCG                                                                             >  SRR4302541.3688329/1‑94 (MQ=60)
                GTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGG                                                                           <  SRR4302541.962207/94‑1 (MQ=60)
                 TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGG                                                                          >  SRR4302541.349128/1‑94 (MQ=60)
                 TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                          <  SRR4302541.1484735/94‑1 (MQ=60)
                 TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                          >  SRR4302541.3632063/1‑94 (MQ=60)
                   AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGG                                                                        >  SRR4302541.7582785/1‑94 (MQ=60)
                      CGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTA                                                                     >  SRR4302541.6554634/1‑94 (MQ=60)
                       GCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTAT                                                                    <  SRR4302541.3632119/94‑1 (MQ=60)
                       GCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTAT                                                                    <  SRR4302541.3632118/94‑1 (MQ=60)
                         GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAG                                                                  >  SRR4302541.3620132/1‑94 (MQ=60)
                         GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAG                                                                  >  SRR4302541.7581801/1‑94 (MQ=60)
                          TCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGT                                                                 <  SRR4302541.5447367/94‑1 (MQ=60)
                          TCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGT                                                                 >  SRR4302541.2111093/1‑94 (MQ=60)
                           CTGTTATTGGCCTTCGCAGTCCCGGTGCTTCTCGCGCGCATCGCCCATACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                                <  SRR4302541.6782323/94‑1 (MQ=44)
                           CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                                >  SRR4302541.5235007/1‑94 (MQ=60)
                           CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                                >  SRR4302541.4621596/1‑94 (MQ=60)
                           CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                                <  SRR4302541.7568199/94‑1 (MQ=60)
                           CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGGG                                                                >  SRR4302541.322848/1‑94 (MQ=60)
                           CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATAGGGTTTGTCGATACCGTGGTGGCGGGCGGCTATAttt                                                                >  SRR4302541.5235008/1‑91 (MQ=60)
                             GTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGCGCC                                                              >  SRR4302541.5184469/1‑94 (MQ=60)
                              TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCA                                                             >  SRR4302541.5569089/1‑94 (MQ=60)
                              TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCA                                                             <  SRR4302541.4089358/94‑1 (MQ=60)
                               TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAC                                                            <  SRR4302541.8654220/94‑1 (MQ=60)
                               TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAC                                                            <  SRR4302541.7074505/94‑1 (MQ=60)
                               TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAC                                                            >  SRR4302541.5541911/1‑94 (MQ=60)
                                ATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACC                                                           >  SRR4302541.13367/1‑94 (MQ=60)
                                ATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACC                                                           >  SRR4302541.5930363/1‑94 (MQ=60)
                                ATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACC                                                           >  SRR4302541.3914069/1‑94 (MQ=60)
                                ATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACC                                                           >  SRR4302541.2238614/1‑94 (MQ=60)
                                 TTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCG                                                          <  SRR4302541.6784934/94‑1 (MQ=60)
                                   AGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGAC                                                        <  SRR4302541.1713910/94‑1 (MQ=60)
                                   AGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGAC                                                        <  SRR4302541.79709/94‑1 (MQ=60)
                                   AGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGAC                                                        >  SRR4302541.1536569/1‑94 (MQ=60)
                                   AGCAGTAGCAATCCCGGTGATTGTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGAC                                                        <  SRR4302541.3189360/94‑1 (MQ=60)
                                    GCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACA                                                       <  SRR4302541.2930811/94‑1 (MQ=60)
                                       TTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGG                                                    <  SRR4302541.5806134/94‑1 (MQ=60)
                                       TTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGG                                                    <  SRR4302541.6467463/94‑1 (MQ=60)
                                       TTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGG                                                    >  SRR4302541.4522297/1‑94 (MQ=60)
                                        TAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGC                                                   <  SRR4302541.2277451/94‑1 (MQ=60)
                                        TAGCAATCCCGGTGATTATCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGC                                                   <  SRR4302541.7911297/94‑1 (MQ=60)
                                         AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                                  <  SRR4302541.3332196/94‑1 (MQ=60)
                                         AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                                  <  SRR4302541.2676003/94‑1 (MQ=60)
                                          GCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGG                                                 <  SRR4302541.8246891/94‑1 (MQ=60)
                                           CAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                                >  SRR4302541.3525855/1‑94 (MQ=60)
                                            AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                               >  SRR4302541.2595415/1‑94 (MQ=60)
                                            AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                               <  SRR4302541.4256907/94‑1 (MQ=60)
                                            AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                               <  SRR4302541.4256906/94‑1 (MQ=60)
                                             ATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATCGGTTTTGTCGATACCGTGATGGCTGGCGGCTATAGTGCCACCGCCAGGGCGGCGG                                              >  SRR4302541.678340/1‑94 (MQ=60)
                                              TCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGG                                             >  SRR4302541.6466860/1‑94 (MQ=60)
                                               CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTC                                            <  SRR4302541.3377743/94‑1 (MQ=60)
                                               CCCGGTGATTCTCGCGCAAAGCGCTCGATCTGCGAGTGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTC                                            <  SRR4302541.8877996/94‑1 (MQ=60)
                                                CCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCG                                           <  SRR4302541.5529343/94‑1 (MQ=60)
                                                CCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCG                                           >  SRR4302541.8042692/1‑94 (MQ=60)
                                                CCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCG                                           <  SRR4302541.5821598/94‑1 (MQ=60)
                                                CCGGTGATTCTCGCGCAAATCGCCCAAACTGCGACGGGTTTTGTCGATCCCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCG                                           <  SRR4302541.5420510/94‑1 (MQ=60)
                                                 CGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGC                                          >  SRR4302541.1909812/1‑94 (MQ=60)
                                                 CGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGC                                          >  SRR4302541.4057009/1‑94 (MQ=60)
                                                  GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         >  SRR4302541.5570126/1‑94 (MQ=60)
                                                  GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         <  SRR4302541.7439251/94‑1 (MQ=60)
                                                  GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         <  SRR4302541.1039184/94‑1 (MQ=60)
                                                  GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         <  SRR4302541.3420921/94‑1 (MQ=60)
                                                  GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         <  SRR4302541.4766366/94‑1 (MQ=60)
                                                  GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                         <  SRR4302541.4766367/94‑1 (MQ=60)
                                                    TGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAT                                       <  SRR4302541.8120003/94‑1 (MQ=60)
                                                    GGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAT                                       <  SRR4302541.7215484/94‑1 (MQ=60)
                                                     GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGGGCTACCGACATGGCGGCcgtccccatc                                      >  SRR4302541.7995984/1‑84 (MQ=60)
                                                      ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTCTCG                                     >  SRR4302541.7071663/1‑94 (MQ=60)
                                                      ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCG                                     >  SRR4302541.7676934/1‑94 (MQ=60)
                                                      ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGAGGCGGTCGGTTTCG                                     >  SRR4302541.7676933/1‑94 (MQ=60)
                                                        TCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGT                                   <  SRR4302541.8619857/94‑1 (MQ=60)
                                                         CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTA                                  <  SRR4302541.541894/94‑1 (MQ=60)
                                                         CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTA                                  >  SRR4302541.3091201/1‑94 (MQ=60)
                                                         CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTA                                  <  SRR4302541.523720/94‑1 (MQ=60)
                                                         CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTA                                  <  SRR4302541.834101/94‑1 (MQ=60)
                                                           CGCGCAAATCGCCCGAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                <  SRR4302541.6446573/94‑1 (MQ=60)
                                                           CGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                <  SRR4302541.8746604/94‑1 (MQ=60)
                                                           CGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                <  SRR4302541.8746605/94‑1 (MQ=60)
                                                            GCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCCGTATCGGTACTT                               >  SRR4302541.7007466/1‑94 (MQ=60)
                                                              GCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCT                             <  SRR4302541.8077182/94‑1 (MQ=60)
                                                               CAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTA                            >  SRR4302541.2333041/1‑94 (MQ=60)
                                                               CAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTA                            >  SRR4302541.1687546/1‑94 (MQ=60)
                                                                 AATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGGTGGCGGGGGGCTATAGTGCCACCGACATGGCGGCGGgccctttcggtacttttttc                          >  SRR4302541.3043005/1‑74 (MQ=60)
                                                                 AATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATC                          <  SRR4302541.5612362/94‑1 (MQ=60)
                                                                  ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                         <  SRR4302541.674231/94‑1 (MQ=60)
                                                                  ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                         >  SRR4302541.4441151/1‑94 (MQ=60)
                                                                  ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                         <  SRR4302541.1760145/94‑1 (MQ=60)
                                                                   TCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCGCCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTG                        <  SRR4302541.7254149/94‑1 (MQ=60)
                                                                     GCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGC                      <  SRR4302541.2239881/94‑1 (MQ=60)
                                                                      CCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCT                     <  SRR4302541.5001126/94‑1 (MQ=60)
                                                                      CCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCT                     >  SRR4302541.3621724/1‑94 (MQ=60)
                                                                       CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                    <  SRR4302541.2603763/94‑1 (MQ=60)
                                                                       CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                    >  SRR4302541.1454138/1‑94 (MQ=60)
                                                                       CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                    <  SRR4302541.8145152/94‑1 (MQ=60)
                                                                         AAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCG                  >  SRR4302541.6142308/1‑94 (MQ=60)
                                                                         AAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCC                  <  SRR4302541.3675143/94‑1 (MQ=60)
                                                                         AAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCC                  <  SRR4302541.3401246/94‑1 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 <  SRR4302541.6383987/94‑1 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 >  SRR4302541.5321284/1‑94 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 <  SRR4302541.783970/94‑1 (MQ=60)
                                                                          AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                 <  SRR4302541.5365136/94‑1 (MQ=60)
                                                                           ACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGG                <  SRR4302541.767754/94‑1 (MQ=60)
                                                                           ACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGG                <  SRR4302541.534056/94‑1 (MQ=60)
                                                                            CTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGC               <  SRR4302541.3249273/94‑1 (MQ=60)
                                                                               CGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAT            >  SRR4302541.4802027/1‑94 (MQ=60)
                                                                                GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATC           >  SRR4302541.8895446/1‑94 (MQ=60)
                                                                                GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATC           >  SRR4302541.55113/1‑94 (MQ=60)
                                                                                GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATC           >  SRR4302541.3750894/1‑94 (MQ=60)
                                                                                   GGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTC        >  SRR4302541.43094/1‑94 (MQ=60)
                                                                                    GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCT       >  SRR4302541.2412841/1‑94 (MQ=60)
                                                                                    GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCT       >  SRR4302541.2412840/1‑94 (MQ=60)
                                                                                     GTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTT      >  SRR4302541.5123108/1‑94 (MQ=60)
                                                                                      TTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATTCTCTTT     >  SRR4302541.1705822/1‑94 (MQ=60)
                                                                                      TTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTT     <  SRR4302541.7612217/94‑1 (MQ=60)
                                                                                      TTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTT     <  SRR4302541.1280561/94‑1 (MQ=60)
                                                                                      TTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTT     <  SRR4302541.2571849/94‑1 (MQ=60)
                                                                                       TTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG    <  SRR4302541.5077617/94‑1 (MQ=60)
                                                                                        TTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGG   <  SRR4302541.7072715/94‑1 (MQ=60)
                                                                                        TTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGG   <  SRR4302541.2894079/94‑1 (MQ=60)
                                                                                         TGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  SRR4302541.739707/1‑94 (MQ=60)
                                                                                         TGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  SRR4302541.6360338/1‑94 (MQ=60)
                                                                                         TGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  SRR4302541.4741665/1‑94 (MQ=60)
                                                                                             |                                                                                         
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT  >  NC_000913/1741481‑1741663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑