Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,746,888 T→C A232A (GCA→GCG)  b1669 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,746,8880TC99.5% 728.3 / ‑4.4 213A232A (GCA→GCG) b1669hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (124/88);  total (124/89)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

Only 100 of 224 total aligned reads displayed.

ATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTAC  >  NC_000913/1746798‑1746977
                                                                                          |                                                                                         
aTTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAgcgc                                                                                         <  2:3711010/93‑1 (MQ=255)
aTTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAgcgc                                                                                         <  1:2316819/93‑1 (MQ=255)
   aaGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggc                                                                                      >  1:4297731/1‑93 (MQ=255)
   aaGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggc                                                                                      <  2:4911776/93‑1 (MQ=255)
    aGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggcg                                                                                     >  1:1844777/1‑93 (MQ=255)
    aGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggcg                                                                                     >  2:2779285/1‑93 (MQ=255)
    aGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggcg                                                                                     >  2:6322447/1‑93 (MQ=255)
     gACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGgcgc                                                                                    <  2:4948435/93‑1 (MQ=255)
     gACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGgcgc                                                                                    <  1:2612493/93‑1 (MQ=255)
      aCGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCt                                                                                   >  2:1998360/1‑93 (MQ=255)
        gATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGAAGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtc                                                                                 >  2:5180842/1‑93 (MQ=255)
         aTAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcc                                                                                >  2:583026/1‑93 (MQ=255)
         aTAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcc                                                                                >  2:3426143/1‑93 (MQ=255)
         aTAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcc                                                                                >  1:1277469/1‑93 (MQ=255)
          tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcct                                                                               >  1:3751041/1‑93 (MQ=255)
          tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcct                                                                               >  2:5351901/1‑93 (MQ=255)
          tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcct                                                                               >  2:2871243/1‑93 (MQ=255)
          tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtccc                                                                               >  1:4097383/1‑92 (MQ=255)
            gCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcctcc                                                                             <  2:4964760/93‑1 (MQ=255)
               gACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTgg                                                                          >  2:2358897/1‑93 (MQ=255)
               gACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTgg                                                                          >  1:1142501/1‑93 (MQ=255)
                aCTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGc                                                                         >  1:3939313/1‑93 (MQ=255)
                 cTCCGGCTCGAATATTGTTTGCGCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGcc                                                                        <  1:6008303/93‑1 (MQ=255)
                  tCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGTGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGcccc                                                                       >  2:1135266/1‑93 (MQ=255)
                  tCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGccc                                                                       <  1:1083207/93‑1 (MQ=255)
                    cGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCga                                                                     <  1:2552302/93‑1 (MQ=255)
                     ggCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCgag                                                                    <  2:5392078/93‑1 (MQ=255)
                      gCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCgaga                                                                   <  1:4943338/93‑1 (MQ=255)
                        tCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaat                                                                 >  1:5890342/1‑93 (MQ=255)
                        tCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaat                                                                 >  2:547298/1‑93 (MQ=255)
                        tCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaat                                                                 >  2:1669852/1‑93 (MQ=255)
                         cGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaata                                                                <  2:3896379/93‑1 (MQ=255)
                         cGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaata                                                                <  1:2297268/93‑1 (MQ=255)
                         cGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaata                                                                >  1:1678637/1‑93 (MQ=255)
                         cGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaata                                                                >  1:5024526/1‑93 (MQ=255)
                          gAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaataa                                                               >  1:64881/1‑93 (MQ=255)
                           aaTATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaataat                                                              >  2:4226993/1‑93 (MQ=255)
                             tatTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTg                                                            >  2:1847114/1‑93 (MQ=255)
                             tatTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTg                                                            >  1:3775224/1‑93 (MQ=255)
                              atTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGa                                                           <  1:860315/93‑1 (MQ=255)
                                tGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGt                                                         <  1:3449781/93‑1 (MQ=255)
                                 gTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTc                                                        <  1:4685027/93‑1 (MQ=255)
                                     tCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGAcc                                                    <  2:812597/93‑1 (MQ=255)
                                      cACCTTCAATGGCGACGGCGAAGCGACCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCa                                                   <  1:3066267/93‑1 (MQ=255)
                                      cACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCa                                                   <  2:3258254/93‑1 (MQ=255)
                                      cACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCa                                                   <  1:366003/93‑1 (MQ=255)
                                      cACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCa                                                   >  1:2166344/1‑93 (MQ=255)
                                       aCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAc                                                  <  2:4328655/93‑1 (MQ=255)
                                       aCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAc                                                  >  2:558524/1‑93 (MQ=255)
                                       aCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAc                                                  >  1:4529526/1‑93 (MQ=255)
                                        ccTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACg                                                 >  1:1264498/1‑93 (MQ=255)
                                         cTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGc                                                >  2:2423916/1‑93 (MQ=255)
                                         cTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGc                                                >  1:4384633/1‑93 (MQ=255)
                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               >  2:2091352/1‑93 (MQ=255)
                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               >  1:32181/1‑93 (MQ=255)
                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               <  1:2380372/93‑1 (MQ=255)
                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               >  2:1194406/1‑93 (MQ=255)
                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               <  1:6397451/93‑1 (MQ=255)
                                           tCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGccc                                              >  1:2000/1‑93 (MQ=255)
                                           tCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGccc                                              <  2:5948843/93‑1 (MQ=255)
                                            cAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCg                                             >  1:3617457/1‑93 (MQ=255)
                                            cAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCg                                             >  2:2689332/1‑93 (MQ=255)
                                             aaTGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGt                                            >  2:3488881/1‑93 (MQ=255)
                                              aTGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTg                                           >  1:132630/1‑93 (MQ=255)
                                                   gACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCAc                                      >  1:4584688/1‑93 (MQ=255)
                                                   gACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCAc                                      <  2:3364533/93‑1 (MQ=255)
                                                    aCGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACt                                     >  2:4461880/1‑93 (MQ=255)
                                                    aCGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACt                                     >  2:2499562/1‑93 (MQ=255)
                                                    aCGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACt                                     >  2:4408613/1‑93 (MQ=255)
                                                       gcgaagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTAcc                                  >  2:4150087/1‑93 (MQ=255)
                                                        cgaagcgaaCTCTTTCGCTGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCGCGCCCGTGCTCACTACCg                                 <  1:6162426/93‑1 (MQ=255)
                                                         caagcaaaCTCTTTCGCCTGCATGATATGCCATCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGt                                <  1:2473923/92‑1 (MQ=255)
                                                          aagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGtt                               <  2:4044466/93‑1 (MQ=255)
                                                          aagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGtt                               <  1:5926900/93‑1 (MQ=255)
                                                          aagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGtt                               <  1:1486371/93‑1 (MQ=255)
                                                          aagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGtt                               >  2:6201572/1‑93 (MQ=255)
                                                            gcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGa                             >  2:3032079/1‑93 (MQ=255)
                                                            gcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGa                             >  2:2969563/1‑93 (MQ=255)
                                                             cgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGaa                            >  2:4583987/1‑93 (MQ=255)
                                                                aCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAAttt                         >  2:186890/1‑93 (MQ=255)
                                                                  tctTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTc                       >  1:5610654/1‑93 (MQ=255)
                                                                   ctTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCa                      >  2:6032115/1‑93 (MQ=255)
                                                                    tttCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAc                     >  1:5036251/1‑93 (MQ=255)
                                                                     ttCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAcc                    <  1:1644169/93‑1 (MQ=255)
                                                                       cGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGc                  >  1:4829570/1‑93 (MQ=255)
                                                                       cGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGc                  >  1:918401/1‑93 (MQ=255)
                                                                        gCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCt                 <  1:255763/93‑1 (MQ=255)
                                                                           gTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGc              >  2:2227579/1‑93 (MQ=255)
                                                                           gTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGc              >  2:1078521/1‑93 (MQ=255)
                                                                            tCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGGTGAATTTTCACCGCTggga             >  1:3295172/1‑91 (MQ=255)
                                                                             cATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCaa            >  1:2879840/1‑93 (MQ=255)
                                                                              aTGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAat           <  2:2188346/93‑1 (MQ=255)
                                                                              aTGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAat           >  2:774018/1‑93 (MQ=255)
                                                                                gATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatga         >  1:5307441/1‑93 (MQ=255)
                                                                                gATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatga         >  1:3135779/1‑93 (MQ=255)
                                                                                 atatGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgat        <  2:5774329/93‑1 (MQ=255)
                                                                                  tatGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgatg       <  1:5729289/93‑1 (MQ=255)
                                                                                   atGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt      <  1:4768091/93‑1 (MQ=255)
                                                                                   atGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt      <  2:2626941/93‑1 (MQ=255)
                                                                                   atGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt      <  1:5257223/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTAC  >  NC_000913/1746798‑1746977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTG  >  NC_000913/1746795‑1746981
                                                                                             |                                                                                             
TTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGC                                                                                               <  SRR4302534.864366/94‑1 (MQ=60)
 TCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCG                                                                                              <  SRR4302534.1584524/94‑1 (MQ=60)
 TCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCG                                                                                              <  SRR4302534.5964169/94‑1 (MQ=60)
   ATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCG                                                                                            <  SRR4302534.2378297/94‑1 (MQ=60)
     TAAGGCGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGC                                                                                          >  SRR4302534.2899925/1‑94 (MQ=60)
     TAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGC                                                                                          >  SRR4302534.1687634/1‑94 (MQ=60)
     TAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGC                                                                                          >  SRR4302534.4927511/1‑94 (MQ=60)
     TAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGC                                                                                          >  SRR4302534.4414185/1‑94 (MQ=60)
      AAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCG                                                                                         >  SRR4302534.1892756/1‑94 (MQ=60)
        GACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCT                                                                                       <  SRR4302534.2681620/94‑1 (MQ=60)
          CGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTC                                                                                     >  SRR4302534.6634707/1‑94 (MQ=60)
           GATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCC                                                                                    >  SRR4302534.1311090/1‑94 (MQ=60)
           GATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCC                                                                                    <  SRR4302534.5709896/94‑1 (MQ=60)
            ATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCT                                                                                   <  SRR4302534.3859673/94‑1 (MQ=60)
            ATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCT                                                                                   >  SRR4302534.3851779/1‑94 (MQ=60)
            ATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCC                                                                                   >  SRR4302534.4207527/1‑94 (MQ=60)
               GCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCT                                                                                <  SRR4302534.2322675/94‑1 (MQ=60)
                CGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTG                                                                               >  SRR4302534.2589215/1‑94 (MQ=60)
                CGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTG                                                                               <  SRR4302534.1907465/94‑1 (MQ=60)
                CGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTG                                                                               >  SRR4302534.2420209/1‑94 (MQ=60)
                 GGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTTG                                                                              >  SRR4302534.3765196/1‑94 (MQ=60)
                 GGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGG                                                                              >  SRR4302534.1172412/1‑94 (MQ=60)
                 GGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGG                                                                              >  SRR4302534.371379/1‑94 (MQ=60)
                  GACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGC                                                                             >  SRR4302534.4044818/1‑94 (MQ=60)
                  GACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGC                                                                             >  SRR4302534.4044819/1‑94 (MQ=60)
                  GACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGC                                                                             >  SRR4302534.2394315/1‑94 (MQ=60)
                    CTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCC                                                                           >  SRR4302534.4995960/1‑94 (MQ=60)
                    CTCCGGCTCGAATATTGTTTGCGCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCC                                                                           <  SRR4302534.6171544/94‑1 (MQ=60)
                     TCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCG                                                                          <  SRR4302534.1111377/94‑1 (MQ=60)
                      CCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGA                                                                         >  SRR4302534.3542390/1‑94 (MQ=60)
                      CCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGA                                                                         <  SRR4302534.3626838/94‑1 (MQ=60)
                       CGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAG                                                                        >  SRR4302534.870965/1‑94 (MQ=60)
                       CGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAG                                                                        <  SRR4302534.2619966/94‑1 (MQ=60)
                       CGGCTCGAATATTGTTTTCACCTTCAATGGCGAAGGCGAAGCGAAATCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAG                                                                        >  SRR4302534.3405537/1‑94 (MQ=60)
                         GCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAA                                                                      <  SRR4302534.5077268/94‑1 (MQ=60)
                         GCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAA                                                                      <  SRR4302534.4755616/94‑1 (MQ=60)
                          CTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAAT                                                                     >  SRR4302534.6050279/1‑94 (MQ=60)
                           TCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATA                                                                    >  SRR4302534.5979752/1‑94 (MQ=60)
                           TCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATA                                                                    >  SRR4302534.1722311/1‑94 (MQ=60)
                           TCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATA                                                                    >  SRR4302534.5160246/1‑94 (MQ=60)
                            caaatattcaTTTCACCTTCAATGGCGACGGCTAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAA                                                                   <  SRR4302534.4650522/84‑1 (MQ=60)
                            CGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAA                                                                   >  SRR4302534.66657/1‑94 (MQ=60)
                            CGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAA                                                                   <  SRR4302534.2358254/94‑1 (MQ=60)
                              AATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATT                                                                 <  SRR4302534.211661/94‑1 (MQ=60)
                               ATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTG                                                                <  SRR4302534.4751069/94‑1 (MQ=60)
                               ATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTG                                                                >  SRR4302534.1470514/1‑94 (MQ=60)
                               ATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTG                                                                >  SRR4302534.3876588/1‑94 (MQ=60)
                                TATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGA                                                               <  SRR4302534.2364023/94‑1 (MQ=60)
                                TATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGA                                                               >  SRR4302534.1663332/1‑94 (MQ=60)
                                TATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGA                                                               >  SRR4302534.606808/1‑94 (MQ=60)
                                 ATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAG                                                              <  SRR4302534.6144760/94‑1 (MQ=60)
                                 ATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAG                                                              <  SRR4302534.882030/94‑1 (MQ=60)
                                  TTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGT                                                             <  SRR4302534.515416/94‑1 (MQ=60)
                                   TGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTC                                                            <  SRR4302534.3201921/94‑1 (MQ=60)
                                   TGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTC                                                            <  SRR4302534.3542513/94‑1 (MQ=60)
                                   TGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTC                                                            >  SRR4302534.1306657/1‑94 (MQ=60)
                                    GTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCG                                                           <  SRR4302534.4812215/94‑1 (MQ=60)
                                    GTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCG                                                           <  SRR4302534.5244722/94‑1 (MQ=60)
                                    GTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCG                                                           <  SRR4302534.4350572/94‑1 (MQ=60)
                                        TCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCA                                                       >  SRR4302534.2223559/1‑94 (MQ=60)
                                         CACCTTCAATGGCGACGGCGAAGCGACCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAC                                                      <  SRR4302534.3146778/94‑1 (MQ=60)
                                         CACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAC                                                      <  SRR4302534.4886833/94‑1 (MQ=60)
                                         CACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAC                                                      >  SRR4302534.4652247/1‑94 (MQ=60)
                                         CACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAC                                                      >  SRR4302534.631192/1‑94 (MQ=60)
                                         CACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAC                                                      >  SRR4302534.4662278/1‑94 (MQ=60)
                                         CACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAC                                                      <  SRR4302534.375341/94‑1 (MQ=60)
                                         CACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAC                                                      >  SRR4302534.1973443/1‑94 (MQ=60)
                                          ACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACG                                                     >  SRR4302534.1297805/1‑94 (MQ=60)
                                          ACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACG                                                     >  SRR4302534.1391988/1‑94 (MQ=60)
                                           CCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGC                                                    <  SRR4302534.1868891/94‑1 (MQ=60)
                                           CCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGC                                                    >  SRR4302534.4502838/1‑94 (MQ=60)
                                            CTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCC                                                   >  SRR4302534.33027/1‑94 (MQ=60)
                                            CTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCC                                                   >  SRR4302534.265895/1‑94 (MQ=60)
                                             TTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCC                                                  <  SRR4302534.6570213/94‑1 (MQ=60)
                                             TTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCC                                                  <  SRR4302534.2443616/94‑1 (MQ=60)
                                             TTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCC                                                  >  SRR4302534.2064/1‑94 (MQ=60)
                                              TCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCG                                                 >  SRR4302534.3714709/1‑94 (MQ=60)
                                               CAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGC                                                >  SRR4302534.2673250/1‑94 (MQ=60)
                                                AATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTG                                               >  SRR4302534.925085/1‑94 (MQ=60)
                                                AATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTG                                               <  SRR4302534.5431660/94‑1 (MQ=60)
                                                AATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTG                                               >  SRR4302534.136127/1‑94 (MQ=60)
                                                AATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTG                                               >  SRR4302534.125921/1‑94 (MQ=60)
                                                AATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTG                                               >  SRR4302534.2620237/1‑94 (MQ=60)
                                                 ATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATACGCCAGCCCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGC                                              <  SRR4302534.748962/94‑1 (MQ=60)
                                                     CGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCAC                                          >  SRR4302534.4709088/1‑94 (MQ=60)
                                                      GACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACT                                         >  SRR4302534.1324633/1‑94 (MQ=60)
                                                        CGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTAC                                       >  SRR4302534.3995384/1‑94 (MQ=60)
                                                          GCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGTTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCG                                     <  SRR4302534.6438650/94‑1 (MQ=60)
                                                           CGAAGCGAACTCTTTCGCTGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCGCGCCCGTGCTCACTACCGT                                    <  SRR4302534.6330044/94‑1 (MQ=60)
                                                            GAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTT                                   >  SRR4302534.5084918/1‑94 (MQ=60)
                                                            GAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTT                                   >  SRR4302534.4447952/1‑94 (MQ=60)
                                                            CAAGCAAACTCTTTCGCCTGCATGATATGCCATCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTT                                   <  SRR4302534.2539660/94‑1 (MQ=60)
                                                             AAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTG                                  <  SRR4302534.6087840/94‑1 (MQ=60)
                                                             AAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTG                                  <  SRR4302534.1525470/94‑1 (MQ=60)
                                                              AGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGA                                 <  SRR4302534.3725485/94‑1 (MQ=60)
                                                                    CTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTC                           <  SRR4302534.952855/94‑1 (MQ=60)
                                                                    CTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTC                           >  SRR4302534.5762441/1‑94 (MQ=60)
                                                                     TCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCA                          >  SRR4302534.3253897/1‑94 (MQ=60)
                                                                      CTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAC                         >  SRR4302534.5172281/1‑94 (MQ=60)
                                                                        TTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCG                       >  SRR4302534.424596/1‑94 (MQ=60)
                                                                        TTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCG                       <  SRR4302534.1687027/94‑1 (MQ=60)
                                                                         TCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGC                      >  SRR4302534.941783/1‑94 (MQ=60)
                                                                         TCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGC                      >  SRR4302534.4960612/1‑94 (MQ=60)
                                                                           GCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTG                    >  SRR4302534.33225/1‑94 (MQ=60)
                                                                           GCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTG                    <  SRR4302534.262428/94‑1 (MQ=60)
                                                                              GTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCA                 >  SRR4302534.5878380/1‑94 (MQ=60)
                                                                              GTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGGTGAATTTTCACCGCTGGGA                 >  SRR4302534.3383080/1‑94 (MQ=60)
                                                                               TCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAA                >  SRR4302534.2955422/1‑94 (MQ=60)
                                                                                  TGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGA             >  SRR4302534.5450647/1‑94 (MQ=60)
                                                                                  TGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGA             >  SRR4302534.3218244/1‑94 (MQ=60)
                                                                                  TGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGA             <  SRR4302534.5084015/94‑1 (MQ=60)
                                                                                   GATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAT            <  SRR4302534.3233865/94‑1 (MQ=60)
                                                                                   GATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAT            <  SRR4302534.6126182/94‑1 (MQ=60)
                                                                                     TATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGT          <  SRR4302534.3097740/94‑1 (MQ=60)
                                                                                     TATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGT          <  SRR4302534.5884537/94‑1 (MQ=60)
                                                                                      ATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTG         <  SRR4302534.4897549/94‑1 (MQ=60)
                                                                                      ATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTG         <  SRR4302534.5399285/94‑1 (MQ=60)
                                                                                        GCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTA       >  SRR4302534.1147100/1‑94 (MQ=60)
                                                                                        GCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTA       >  SRR4302534.2493004/1‑94 (MQ=60)
                                                                                         CCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGTTGTGTAC      <  SRR4302534.5505439/94‑1 (MQ=60)
                                                                                         CCAGCACGGCGCTTCCTACTGGCCCGAGAATTGTTGAGTCGACCACGAAAATGCTCACTACCGTTGAGTTTACACCGCTGGCAATGATGTGTAA      <  SRR4302534.6653604/94‑1 (MQ=38)
                                                                                          CAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACA     >  SRR4302534.6645768/1‑94 (MQ=60)
                                                                                          CAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACA     >  SRR4302534.3920699/1‑94 (MQ=60)
                                                                                             CGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTG  <  SRR4302534.1051179/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTG  >  NC_000913/1746795‑1746981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑