Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,626	T→C	E180E (GAA→GAG)	rspA ←	starvation sensing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,626	0	T	C	100.0%	753.1 / NA	208	E180E (GAA→GAG)	rspA	starvation sensing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (112/96); total (112/96)

Only 100 of 231 total aligned reads displayed.

CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC  >  NC_000913/1652536‑1652715

 agcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                                        >  2:2021794/1‑93 (MQ=255)

 agcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                                        <  2:6054568/93‑1 (MQ=255)

  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                       >  2:1349599/1‑93 (MQ=255)

  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                       >  1:6007143/1‑93 (MQ=255)

  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                       >  2:4912242/1‑93 (MQ=255)

   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  2:4775810/93‑1 (MQ=255)

   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      >  2:5314400/1‑93 (MQ=255)

   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  1:5084684/93‑1 (MQ=255)

    aaaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTcc                                                                                     <  1:3773766/93‑1 (MQ=255)

    aaaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTcc                                                                                     >  2:4824705/1‑93 (MQ=255)

    aaaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTcc                                                                                     <  1:2372047/93‑1 (MQ=255)

     aaTGTTCATTAAAACCAAACTTTTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGATGTATTTCTCCGTCGACCACAGCTGCTCTTCCg                                                                                    >  2:4757572/1‑93 (MQ=255)

     aaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCg                                                                                    <  2:3914921/93‑1 (MQ=255)

      aTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCgg                                                                                   >  1:3844925/1‑93 (MQ=255)

      aTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCgg                                                                                   >  1:3332709/1‑93 (MQ=255)

            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                             >  1:1702568/1‑93 (MQ=255)

                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        <  1:904559/93‑1 (MQ=255)

                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       >  1:4843115/1‑93 (MQ=255)

                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       <  1:581417/93‑1 (MQ=255)

                   ccAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                                      >  1:4162698/1‑93 (MQ=255)

                   ccAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                                      <  1:3871405/93‑1 (MQ=255)

                   ccAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                                      <  2:183961/93‑1 (MQ=255)

                     aaaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGt                                                                    <  1:3030667/93‑1 (MQ=255)

                      atcTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGtt                                                                   <  1:1006721/91‑1 (MQ=255)

                       aCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTg                                                                  <  2:4189433/93‑1 (MQ=255)

                        cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCTTCGACCACAGCTGCTCTTCCGGCCACTGTTCTTTGGTTGc                                                                 >  2:5480322/1‑93 (MQ=255)

                        cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGc                                                                 >  1:2690837/1‑93 (MQ=255)

                         ttGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCg                                                                >  2:1205849/1‑93 (MQ=255)

                         ttGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCg                                                                >  2:2462311/1‑93 (MQ=255)

                          tGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCgg                                                               <  1:1976762/93‑1 (MQ=255)

                           tttACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCAACTGTCCTTTGGTTGCggg                                                              <  2:4977262/92‑1 (MQ=255)

                           gTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCggg                                                              >  2:2906443/1‑93 (MQ=255)

                           gTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCggg                                                              >  2:4413152/1‑93 (MQ=255)

                            ttACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGt                                                             >  1:469979/1‑93 (MQ=255)

                             tACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGtt                                                            >  2:1260551/1‑93 (MQ=255)

                              aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTc                                                           >  2:1034766/1‑93 (MQ=255)

                              aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTc                                                           <  1:485749/93‑1 (MQ=255)

                                gTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                                         >  1:5657762/1‑93 (MQ=255)

                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       <  2:252961/93‑1 (MQ=255)

                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       <  2:4769176/93‑1 (MQ=255)

                                    cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGc                                                     >  1:4123876/1‑93 (MQ=255)

                                     gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGcc                                                    <  2:4928205/93‑1 (MQ=255)

                                     gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGcc                                                    >  2:582705/1‑93 (MQ=255)

                                         cAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGac                                                <  1:4268324/93‑1 (MQ=255)

                                          aaaCGATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCACacc                                               <  1:3972990/93‑1 (MQ=255)

                                          aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                               >  1:3720547/1‑93 (MQ=255)

                                          aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                               <  2:4251994/93‑1 (MQ=255)

                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                              <  1:5384189/93‑1 (MQ=255)

                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                              >  2:2664216/1‑93 (MQ=255)

                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             <  2:3962046/93‑1 (MQ=255)

                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             >  1:5610798/1‑93 (MQ=255)

                                             cAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttt                                            >  2:1582634/1‑93 (MQ=255)

                                             cAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttt                                            >  2:366440/1‑93 (MQ=255)

                                              aaTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttta                                           >  1:202068/1‑93 (MQ=255)

                                              aaTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttta                                           >  1:2927485/1‑93 (MQ=255)

                                               aTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttac                                          >  2:5712815/1‑93 (MQ=255)

                                                tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacc                                         <  1:5699251/93‑1 (MQ=255)

                                                 ttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacct                                        <  1:1348326/93‑1 (MQ=255)

                                                 ttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacct                                        <  1:5428940/93‑1 (MQ=255)

                                                     gCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCg                                    >  2:676175/1‑93 (MQ=255)

                                                     gCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCg                                    >  1:3646550/1‑93 (MQ=255)

                                                      cATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGACCTTTGGTTGCGGGCTCATAAGCCAGACCTTTACCTTTCgc                                   >  1:1159699/1‑92 (MQ=255)

                                                       aTGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCAGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCTAGACCTTTACCTTTCGAc                                  >  2:3377120/1‑93 (MQ=255)

                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 <  2:156174/93‑1 (MQ=255)

                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 <  1:4408932/93‑1 (MQ=255)

                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 >  1:5007065/1‑93 (MQ=255)

                                                         gAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAt                                <  1:1265863/93‑1 (MQ=255)

                                                          aaaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATg                               <  2:1169753/93‑1 (MQ=255)

                                                          aaaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATg                               >  2:6037105/1‑93 (MQ=255)

                                                           aaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc                              <  1:4610344/93‑1 (MQ=255)

                                                           aaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc                              >  1:4620532/1‑93 (MQ=255)

                                                            aTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc                             <  1:2515216/93‑1 (MQ=255)

                                                             tCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCg                            >  2:1105146/1‑93 (MQ=255)

                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCTAGACCTTTACCTTTCGACATGCCGTa                          <  2:5034588/93‑1 (MQ=255)

                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa                          >  2:1710399/1‑93 (MQ=255)

                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa                          <  2:5598097/93‑1 (MQ=255)

                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         <  1:5249813/93‑1 (MQ=255)

                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCTCTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTCCCTTTCGACATGCCGTAggt                       <  1:3432353/93‑1 (MQ=255)

                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt                       <  1:5399258/93‑1 (MQ=255)

                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt                       <  1:4333476/93‑1 (MQ=255)

                                                                   tATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtg                      <  2:5425275/93‑1 (MQ=255)

                                                                   gaTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtg                      <  2:2985819/92‑1 (MQ=255)

                                                                         tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTc                >  1:2177200/1‑93 (MQ=255)

                                                                           cGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAt              >  1:713407/1‑93 (MQ=255)

                                                                           cGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAt              <  1:4979223/93‑1 (MQ=255)

                                                                            gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATa             <  1:3466470/93‑1 (MQ=255)

                                                                             gcGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAc            <  2:5324195/92‑1 (MQ=255)

                                                                              cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAcc           >  2:5682218/1‑93 (MQ=255)

                                                                              cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAcc           <  2:285544/93‑1 (MQ=255)

                                                                               gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCa          >  2:3119104/1‑93 (MQ=255)

                                                                                aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAg         <  2:4602661/93‑1 (MQ=255)

                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        >  2:4139432/1‑93 (MQ=255)

                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        <  1:1671148/93‑1 (MQ=255)

                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        <  1:166013/93‑1 (MQ=255)

                                                                                  gacaGCTGCTCTTCCGGGCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGTCCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAggg       <  2:4178664/92‑1 (MQ=255)

                                                                                    caGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAt     >  2:2812675/1‑93 (MQ=255)

                                                                                      gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTc   >  2:1463463/1‑93 (MQ=255)

                                                                                      gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTc   >  1:1957369/1‑93 (MQ=255)

                                                                                       cTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTcc  <  2:3537489/93‑1 (MQ=255)

                                                                                       cTGCTCTTCCGGCCACTGTCCTTTGGATGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTcc  >  1:1922486/1‑93 (MQ=255)

CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC  >  NC_000913/1652536‑1652715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  NC_000913/1652534‑1652719

TGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCT                                                                                              >  SRR4302532.5718368/1‑94 (MQ=60)

TGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCT                                                                                              >  SRR4302532.5872302/1‑94 (MQ=60)

   AGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTT                                                                                           >  SRR4302532.6214574/1‑94 (MQ=60)

    GCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTC                                                                                          <  SRR4302532.5521288/94‑1 (MQ=60)

     CAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCC                                                                                         <  SRR4302532.5259513/94‑1 (MQ=60)

      AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                        >  SRR4302532.6005638/1‑94 (MQ=60)

      AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                        <  SRR4302532.2451130/94‑1 (MQ=60)

      AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                        <  SRR4302532.3900830/94‑1 (MQ=60)

       AATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGG                                                                                       >  SRR4302532.4888138/1‑94 (MQ=60)

       AATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGG                                                                                       >  SRR4302532.3443858/1‑94 (MQ=60)

       AATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGG                                                                                       >  SRR4302532.3974745/1‑94 (MQ=60)

        ATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGC                                                                                      >  SRR4302532.886524/1‑94 (MQ=60)

          GTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCA                                                                                    >  SRR4302532.2030253/1‑94 (MQ=60)

            TTATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTATACGTCGAACGCAGCTGCTCTTTCGGCCACT                                                                                  >  SRR4302532.3849292/1‑94 (MQ=60)

             CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                 >  SRR4302532.1759986/1‑94 (MQ=60)

              ATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGT                                                                                <  SRR4302532.5935327/94‑1 (MQ=60)

              ATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGT                                                                                <  SRR4302532.2052222/94‑1 (MQ=60)

                   AACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCTTTT                                                                           >  SRR4302532.3107929/1‑94 (MQ=60)

                   AACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTT                                                                           <  SRR4302532.935419/94‑1 (MQ=60)

                   AACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTT                                                                           >  SRR4302532.5009831/1‑94 (MQ=60)

                    ACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTG                                                                          <  SRR4302532.601690/94‑1 (MQ=60)

                    ACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTG                                                                          >  SRR4302532.4302304/1‑94 (MQ=60)

                     CCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGG                                                                         <  SRR4302532.4002049/94‑1 (MQ=60)

                      CAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGT                                                                        >  SRR4302532.5109809/1‑94 (MQ=60)

                       AAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTT                                                                       <  SRR4302532.3130691/94‑1 (MQ=60)

                       AAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTT                                                                       <  SRR4302532.2487676/94‑1 (MQ=60)

                        ATCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTG                                                                      <  SRR4302532.1040969/94‑1 (MQ=60)

                         ACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGC                                                                     >  SRR4302532.2781219/1‑94 (MQ=60)

                            TGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGG                                                                  <  SRR4302532.2042417/94‑1 (MQ=60)

                            TGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGG                                                                  >  SRR4302532.3951348/1‑94 (MQ=60)

                             GTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGT                                                                 >  SRR4302532.486346/1‑94 (MQ=60)

                                ACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCA                                                              <  SRR4302532.502710/94‑1 (MQ=60)

                                 CGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAT                                                             >  SRR4302532.5854802/1‑94 (MQ=60)

                                 CGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAT                                                             <  SRR4302532.2161221/94‑1 (MQ=60)

                                    ACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAG                                                          <  SRR4302532.5640462/94‑1 (MQ=60)

                                    ACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAG                                                          >  SRR4302532.959251/1‑94 (MQ=60)

                                     CCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGC                                                         >  SRR4302532.4262269/1‑94 (MQ=60)

                                      CGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTTGTTGCGGGTTCATAAGCC                                                        <  SRR4302532.432959/94‑1 (MQ=60)

                                        CGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAG                                                      >  SRR4302532.4840135/1‑94 (MQ=60)

                                        CGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAG                                                      >  SRR4302532.3570680/1‑94 (MQ=60)

                                        CGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAG                                                      <  SRR4302532.5883409/94‑1 (MQ=60)

                                        CGTCAAACAATATCGGCTTAAACACGAGGTATTTCTCCGTCGGCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAG                                                      <  SRR4302532.5829823/94‑1 (MQ=60)

                                           CAAACAATTTCGGCATGAAATCGGGGTATTTATACGTCGACCACAGATGCTCTTCCGGCCACTGTCCTTTTGTTGAGGGTTCtcttgtctaaat                                                   >  SRR4302532.1943474/1‑82 (MQ=55)

                                           CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACC                                                   <  SRR4302532.4412032/94‑1 (MQ=60)

                                           CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACC                                                   >  SRR4302532.1718283/1‑94 (MQ=60)

                                           CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACC                                                   >  SRR4302532.3845455/1‑94 (MQ=60)

                                            AAACGATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCACACCT                                                  <  SRR4302532.4106816/94‑1 (MQ=60)

                                            AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                  <  SRR4302532.6123741/94‑1 (MQ=60)

                                            AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTATTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                  >  SRR4302532.1714999/1‑94 (MQ=60)

                                             AACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTT                                                 >  SRR4302532.6298643/1‑94 (MQ=60)

                                             AACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTT                                                 <  SRR4302532.2701373/94‑1 (MQ=60)

                                             AACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTT                                                 >  SRR4302532.374913/1‑94 (MQ=60)

                                             AACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTT                                                 >  SRR4302532.5805523/1‑94 (MQ=60)

                                             AACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTT                                                 <  SRR4302532.5570684/94‑1 (MQ=60)

                                              ACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTT                                                >  SRR4302532.521052/1‑94 (MQ=60)

                                               CAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTA                                               >  SRR4302532.3024875/1‑94 (MQ=60)

                                               CAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTA                                               >  SRR4302532.208961/1‑94 (MQ=60)

                                                AATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTAC                                              >  SRR4302532.4096535/1‑94 (MQ=60)

                                                  TTTCTGCATGAAATCGAGGTATTTCTCCCTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCT                                            <  SRR4302532.2882760/94‑1 (MQ=60)

                                                  TTTCGGCATGAAATCGGGGTATTCCTCAGTCGACCACAGCTGCTCTCCCGGCCACTGTCATTTGCTTGCGGGTTCATAAGCCAGACCTTTACCT                                            <  SRR4302532.4238507/94‑1 (MQ=60)

                                                  TTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCT                                            <  SRR4302532.5331921/94‑1 (MQ=60)

                                                  TTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCT                                            <  SRR4302532.5898043/94‑1 (MQ=60)

                                                   TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           <  SRR4302532.5617094/94‑1 (MQ=60)

                                                   TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           >  SRR4302532.1295783/1‑94 (MQ=60)

                                                   TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           <  SRR4302532.1393404/94‑1 (MQ=60)

                                                     CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         >  SRR4302532.4226690/1‑94 (MQ=60)

                                                     CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         <  SRR4302532.1350558/94‑1 (MQ=60)

                                                      GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCG                                        >  SRR4302532.3768923/1‑94 (MQ=60)

                                                      GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCG                                        <  SRR4302532.4840303/94‑1 (MQ=60)

                                                       GCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGACCTTTGGTTGCGGGCTCATAAGCCAGACCTTTACCTTTCGC                                       >  SRR4302532.1198448/1‑94 (MQ=60)

                                                        CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                      >  SRR4302532.5021527/1‑94 (MQ=60)

                                                        CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                      <  SRR4302532.4290070/94‑1 (MQ=60)

                                                         ATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACA                                     >  SRR4302532.5178673/1‑94 (MQ=60)

                                                          TGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAT                                    <  SRR4302532.4559113/94‑1 (MQ=60)

                                                          TGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAT                                    <  SRR4302532.5580371/94‑1 (MQ=60)

                                                          TGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAT                                    <  SRR4302532.5166566/94‑1 (MQ=60)

                                                           GAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATG                                   <  SRR4302532.1067745/94‑1 (MQ=60)

                                                           GAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATG                                   <  SRR4302532.1308204/94‑1 (MQ=60)

                                                           GAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATG                                   <  SRR4302532.3983940/94‑1 (MQ=60)

                                                           GAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTAATAAGCCAGACCTTTGCCTTTCGACATG                                   <  SRR4302532.4234423/94‑1 (MQ=60)

                                                            AAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGC                                  >  SRR4302532.4778090/1‑94 (MQ=60)

                                                             AATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCC                                 <  SRR4302532.4767532/94‑1 (MQ=60)

                                                             AATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCC                                 >  SRR4302532.2594122/1‑94 (MQ=60)

                                                              ATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCG                                >  SRR4302532.2384346/1‑94 (MQ=60)

                                                              ATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCG                                <  SRR4302532.2599139/94‑1 (MQ=60)

                                                                CGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTA                              >  SRR4302532.92391/1‑94 (MQ=60)

                                                                CGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGCA                              >  SRR4302532.5593828/1‑94 (MQ=60)

                                                                 GAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                             <  SRR4302532.1667969/94‑1 (MQ=60)

                                                                 GAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                             >  SRR4302532.4765797/1‑94 (MQ=60)

                                                                 GAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                             >  SRR4302532.1884850/1‑94 (MQ=60)

                                                                 GAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                             <  SRR4302532.32580/94‑1 (MQ=60)

                                                                 GAGGGATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGAGGGTTCATAAGCCCGACCTTTACTTTTCGACATGCCGTAG                             >  SRR4302532.4540609/1‑94 (MQ=60)

                                                                  AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                            >  SRR4302532.4413408/1‑94 (MQ=60)

                                                                  AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                            <  SRR4302532.5430788/94‑1 (MQ=60)

                                                                    GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCTCTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTCCCTTTCGACATGCCGTAGGTG                          <  SRR4302532.3547350/94‑1 (MQ=60)

                                                                    GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                          <  SRR4302532.4479876/94‑1 (MQ=60)

                                                                    GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                          <  SRR4302532.560325/94‑1 (MQ=60)

                                                                    GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                          <  SRR4302532.5026142/94‑1 (MQ=60)

                                                                    GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                          <  SRR4302532.5586341/94‑1 (MQ=60)

                                                                      ATTTTTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTTCTTTGGTTGCGGGTTCATCAGCCAGAACTTTACCTTTCGACATGCCGTAGGTGGT                        >  SRR4302532.2940223/1‑94 (MQ=60)

                                                                       TTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGGT                       >  SRR4302532.3743016/1‑94 (MQ=60)

                                                                        TTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTT                      >  SRR4302532.3128459/1‑94 (MQ=60)

                                                                        TTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGGGGTTT                      >  SRR4302532.2045824/1‑94 (MQ=60)

                                                                         TCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTT                     >  SRR4302532.529790/1‑94 (MQ=60)

                                                                         TCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTT                     >  SRR4302532.1755045/1‑94 (MQ=60)

                                                                          CTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTC                    >  SRR4302532.2249910/1‑94 (MQ=60)

                                                                           TCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCA                   <  SRR4302532.4770518/94‑1 (MQ=60)

                                                                            CCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAT                  >  SRR4302532.737782/1‑94 (MQ=60)

                                                                            CCGTCGACCACAGCCGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAT                  <  SRR4302532.5218726/94‑1 (MQ=60)

                                                                             CGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATA                 >  SRR4302532.3905101/1‑94 (MQ=60)

                                                                             CGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATA                 <  SRR4302532.5149903/94‑1 (MQ=60)

                                                                              GTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAC                <  SRR4302532.3582572/94‑1 (MQ=60)

                                                                              GTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAC                <  SRR4302532.3538585/94‑1 (MQ=60)

                                                                                CGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATTCCGTAGGTGGTTTTCATACCA              <  SRR4302532.3830582/94‑1 (MQ=60)

                                                                                   CCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGG           <  SRR4302532.1895635/94‑1 (MQ=60)

                                                                                   CCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGG           <  SRR4302532.1727695/94‑1 (MQ=60)

                                                                                   CCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGG           <  SRR4302532.171580/94‑1 (MQ=60)

                                                                                   CCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGG           >  SRR4302532.3131901/1‑94 (MQ=60)

                                                                                       AGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTC       >  SRR4302532.2022389/1‑94 (MQ=60)

                                                                                       AGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTC       >  SRR4302532.4531794/1‑94 (MQ=60)

                                                                                        GCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC      >  SRR4302532.1986433/1‑94 (MQ=60)

                                                                                        GCTGCTCTTCCGGCCACTGTCCTTTGGATGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC      >  SRR4302532.1986432/1‑94 (MQ=60)

                                                                                          TGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGC    >  SRR4302532.6085142/1‑94 (MQ=60)

                                                                                            CTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  SRR4302532.763174/1‑94 (MQ=60)

TGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  NC_000913/1652534‑1652719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`