Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,652,626 T→C E180E (GAA→GAG)  rspA ← starvation sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,652,6260TC99.5% 740.3 / NA 201E180E (GAA→GAG) rspAstarvation sensing protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (99/101);  total (99/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTC  >  NC_000913/1652536‑1652714
                                                                                          |                                                                                        
  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                      <  2:3501212/93‑1 (MQ=255)
  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                      >  1:1629347/1‑93 (MQ=255)
  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                      <  1:1684398/93‑1 (MQ=255)
  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                      >  1:5127349/1‑93 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                     <  2:2659347/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                     <  2:5390204/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                     <  2:2103053/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                     <  2:4065616/93‑1 (MQ=255)
    aaaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTcc                                                                                    <  1:4020859/93‑1 (MQ=255)
    aaaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTcc                                                                                    <  1:1950832/93‑1 (MQ=255)
        gTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGcc                                                                                >  1:5685987/1‑93 (MQ=255)
          tCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                                              >  1:4974368/1‑93 (MQ=255)
          tCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAc                                                                              <  1:6160251/93‑1 (MQ=255)
           cATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACt                                                                             <  1:4764563/93‑1 (MQ=255)
           cATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACt                                                                             <  1:2918731/93‑1 (MQ=255)
            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                            >  2:4479432/1‑93 (MQ=255)
            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                            >  2:1962920/1‑93 (MQ=255)
             ttAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGt                                                                           >  2:5976184/1‑93 (MQ=255)
               aaaaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTcc                                                                         >  2:5660741/1‑93 (MQ=255)
                aaaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCt                                                                        >  1:3783278/1‑93 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGATCACAGCTGCTCTTCCCGCCACTGTCCtt                                                                       >  1:3137880/1‑93 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                      >  2:5336956/1‑93 (MQ=255)
                     aaaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCTGGCCGCTGTCCTTTGGt                                                                   >  1:5584101/1‑93 (MQ=255)
                     aaaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGt                                                                   <  2:2148865/93‑1 (MQ=255)
                     aaaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGt                                                                   <  1:2613061/93‑1 (MQ=255)
                       aCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACAACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTg                                                                 >  2:6221920/1‑93 (MQ=255)
                        cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGc                                                                <  1:6620276/93‑1 (MQ=255)
                        cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGc                                                                >  1:2820830/1‑93 (MQ=255)
                          tGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCgg                                                              >  1:1224157/1‑93 (MQ=255)
                           gTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCggg                                                             <  1:1854781/93‑1 (MQ=255)
                            ttACGTACTGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGt                                                            >  1:6447103/1‑93 (MQ=255)
                            ttACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGt                                                            <  2:2167622/93‑1 (MQ=255)
                            ttACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGt                                                            <  2:1950234/93‑1 (MQ=255)
                             tACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGtt                                                           >  2:6070968/1‑93 (MQ=255)
                                gTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                                        <  1:42387/93‑1 (MQ=255)
                                gTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                                        >  2:4109080/1‑93 (MQ=255)
                                 tACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATa                                                       <  1:4938083/93‑1 (MQ=255)
                                 tACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATa                                                       >  2:3219549/1‑93 (MQ=255)
                                 tACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATa                                                       <  1:1834056/93‑1 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                      >  1:3819584/1‑93 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                      >  1:917575/1‑93 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                      <  2:2646970/93‑1 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTGCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                      <  2:6020842/93‑1 (MQ=255)
                                      cgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCa                                                  >  1:4656613/1‑93 (MQ=255)
                                        tCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGa                                                >  1:3298977/1‑93 (MQ=255)
                                          aaaCAATTTCGGCATGAAATCGAGGTGTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                              >  2:5769251/1‑93 (MQ=255)
                                          aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                              <  1:2057872/93‑1 (MQ=255)
                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                             >  2:3898960/1‑93 (MQ=255)
                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                             <  2:2262303/93‑1 (MQ=255)
                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                             <  2:6624061/93‑1 (MQ=255)
                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                             >  1:113212/1‑93 (MQ=255)
                                              aaTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttta                                          <  1:2687097/93‑1 (MQ=255)
                                               aTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttac                                         >  2:6390949/1‑93 (MQ=255)
                                                tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTTATAAGCCAGacctttacc                                        >  1:2561978/1‑93 (MQ=255)
                                                tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacc                                        <  2:775956/93‑1 (MQ=255)
                                                tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacc                                        >  2:1569015/1‑93 (MQ=255)
                                                 ttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacct                                       <  1:1010814/93‑1 (MQ=255)
                                                  tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                                      <  2:4390341/93‑1 (MQ=255)
                                                   cGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttaccttt                                     <  2:630258/93‑1 (MQ=255)
                                                   cGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttaccttt                                     <  2:3175019/93‑1 (MQ=255)
                                                    ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                                    <  2:5118730/93‑1 (MQ=255)
                                                     gCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCGTTACCTTTCg                                   >  1:5906531/1‑93 (MQ=255)
                                                      cATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGa                                  >  1:2996237/1‑93 (MQ=255)
                                                          aaaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATg                              <  2:683089/93‑1 (MQ=255)
                                                          aaaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATg                              >  1:3513872/1‑93 (MQ=255)
                                                            aTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc                            <  2:165340/93‑1 (MQ=255)
                                                            aTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc                            <  1:3556854/93‑1 (MQ=255)
                                                             tCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCg                           <  2:2899750/93‑1 (MQ=255)
                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa                         <  2:2822616/93‑1 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                        <  2:1687271/93‑1 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                        >  1:6522691/1‑93 (MQ=255)
                                                                 ggTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAgg                       <  1:715783/93‑1 (MQ=255)
                                                                 ggTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAgg                       <  1:3167303/93‑1 (MQ=255)
                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt                      <  1:4525379/93‑1 (MQ=255)
                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt                      <  1:4727667/93‑1 (MQ=255)
                                                                    aTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg                    <  2:4031878/93‑1 (MQ=255)
                                                                     tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATCAGGCAGACCTTTATCTTTCGACATGTCGTAggtggt                   >  2:3711237/1‑93 (MQ=255)
                                                                     tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt                   >  2:1254996/1‑93 (MQ=255)
                                                                     ttcctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt                   <  2:4738745/90‑1 (MQ=255)
                                                                      ttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGtt                  >  2:6465311/1‑93 (MQ=255)
                                                                       tctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGttt                 >  2:1014339/1‑93 (MQ=255)
                                                                         tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTc               >  1:5725135/1‑93 (MQ=255)
                                                                          ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCa              <  1:5295835/93‑1 (MQ=255)
                                                                           cGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAt             <  1:1909111/93‑1 (MQ=255)
                                                                              cGACCACAGCTGCTCTTCCCGCCACTGTCCTTTGGTTGCGGGTTCCTAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGCTTTCATAcc          >  1:6066495/1‑93 (MQ=255)
                                                                               gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCa         >  1:5224077/1‑93 (MQ=255)
                                                                                aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAg        >  2:5220614/1‑93 (MQ=255)
                                                                                aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAg        >  2:3042348/1‑93 (MQ=255)
                                                                                aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAg        <  2:6237665/93‑1 (MQ=255)
                                                                                aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAg        <  1:5655794/93‑1 (MQ=255)
                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg       >  2:1742887/1‑93 (MQ=255)
                                                                                  cacaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAggg      >  2:4614288/1‑93 (MQ=255)
                                                                                   acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGa     <  2:6343281/93‑1 (MQ=255)
                                                                                   acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGa     <  1:2532203/93‑1 (MQ=255)
                                                                                   acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGGTTTCATACCAGGGa     >  1:6258355/1‑93 (MQ=255)
                                                                                    caGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAt    <  2:5048538/93‑1 (MQ=255)
                                                                                     aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAtt   >  2:5857063/1‑93 (MQ=255)
                                                                                     aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAtt   >  2:4200921/1‑93 (MQ=255)
                                                                                      gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTc  >  2:2706287/1‑93 (MQ=255)
                                                                                      gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGACAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTc  >  2:3555761/1‑93 (MQ=255)
                                                                                          |                                                                                        
CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTC  >  NC_000913/1652536‑1652714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCA  >  NC_000913/1652533‑1652718
                                                                                             |                                                                                            
ATGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGC                                                                                              >  SRR4302547.1736550/1‑94 (MQ=60)
 TGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCT                                                                                             >  SRR4302547.4140229/1‑94 (MQ=60)
  GCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTC                                                                                            <  SRR4302547.2382826/94‑1 (MQ=60)
   CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCT                                                                                           <  SRR4302547.2819255/94‑1 (MQ=60)
    AGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTT                                                                                          >  SRR4302547.5233001/1‑94 (MQ=60)
    AGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTT                                                                                          >  SRR4302547.1661583/1‑94 (MQ=60)
     GCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTC                                                                                         <  SRR4302547.1717550/94‑1 (MQ=60)
      CAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCC                                                                                        >  SRR4302547.5939697/1‑94 (MQ=60)
      CAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCC                                                                                        <  SRR4302547.5089311/94‑1 (MQ=60)
       AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                       <  SRR4302547.4102553/94‑1 (MQ=60)
       AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                       <  SRR4302547.6383979/94‑1 (MQ=60)
       AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                       <  SRR4302547.28945/94‑1 (MQ=60)
       AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                       >  SRR4302547.6405742/1‑94 (MQ=60)
       AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                       <  SRR4302547.1989519/94‑1 (MQ=60)
          TGTTCATTAAAACCAATCTTGTGAGGTACCGCGGCAAGCAATTTCGGCATGAAATCGAGGCATTTCTCCGTCGACCACAGCTGCTTTTCCGGCC                                                                                    >  SRR4302547.4912147/1‑94 (MQ=60)
          TGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCC                                                                                    >  SRR4302547.5803393/1‑94 (MQ=60)
          TGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCC                                                                                    <  SRR4302547.576856/94‑1 (MQ=60)
            TTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCAC                                                                                  >  SRR4302547.5077266/1‑94 (MQ=60)
             TCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACT                                                                                 <  SRR4302547.6288096/94‑1 (MQ=60)
              CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                <  SRR4302547.2976940/94‑1 (MQ=60)
              CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                >  SRR4302547.4896717/1‑94 (MQ=60)
              CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                <  SRR4302547.4863262/94‑1 (MQ=60)
              CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                >  SRR4302547.1332065/1‑94 (MQ=60)
                  AAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCT                                                                            >  SRR4302547.3860421/1‑94 (MQ=60)
                   AAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGATCACAGCTGCTCTTCCCGCCACTGTCCTT                                                                           >  SRR4302547.3200199/1‑94 (MQ=60)
                   AAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTT                                                                           <  SRR4302547.4251352/94‑1 (MQ=60)
                      CCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGG                                                                        >  SRR4302547.4858733/1‑94 (MQ=60)
                       CAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCTGGCCGCTGTCCTTTGGT                                                                       >  SRR4302547.5699276/1‑94 (MQ=60)
                        AAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTT                                                                      >  SRR4302547.5633960/1‑94 (MQ=60)
                        AAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTT                                                                      <  SRR4302547.2665719/94‑1 (MQ=60)
                         AACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTG                                                                     <  SRR4302547.3994537/94‑1 (MQ=60)
                          ACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGC                                                                    >  SRR4302547.2877256/1‑94 (MQ=60)
                           CTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCG                                                                   <  SRR4302547.6758071/94‑1 (MQ=60)
                            TTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGG                                                                  >  SRR4302547.1248847/1‑94 (MQ=60)
                             TGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGG                                                                 >  SRR4302547.5428185/1‑94 (MQ=60)
                              GTTACGTACTGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGT                                                                >  SRR4302547.6581428/1‑94 (MQ=60)
                              GTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGT                                                                <  SRR4302547.1891432/94‑1 (MQ=60)
                               TTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTT                                                               <  SRR4302547.6059303/94‑1 (MQ=60)
                                TACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTC                                                              >  SRR4302547.6278118/1‑94 (MQ=60)
                                TACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTC                                                              >  SRR4302547.3459910/1‑94 (MQ=60)
                                 ACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCA                                                             <  SRR4302547.1946062/94‑1 (MQ=60)
                                  CGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAT                                                            >  SRR4302547.2983754/1‑94 (MQ=60)
                                  CGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAT                                                            >  SRR4302547.2032386/1‑94 (MQ=60)
                                   GTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATA                                                           <  SRR4302547.43165/94‑1 (MQ=60)
                                   GTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATA                                                           <  SRR4302547.265661/94‑1 (MQ=60)
                                    TACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAA                                                          <  SRR4302547.5040359/94‑1 (MQ=60)
                                    TACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAA                                                          >  SRR4302547.3897416/1‑94 (MQ=60)
                                    TACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAA                                                          >  SRR4302547.935281/1‑94 (MQ=60)
                                    TACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAA                                                          <  SRR4302547.1679417/94‑1 (MQ=60)
                                    TACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAA                                                          <  SRR4302547.1870317/94‑1 (MQ=60)
                                     ACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAG                                                         >  SRR4302547.4240078/1‑94 (MQ=60)
                                     ACCGCGTCAAACAATTTCGGCATGAAATCGAGCAATTCCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAG                                                         <  SRR4302547.1016283/94‑1 (MQ=60)
                                       CGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCATCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCC                                                       <  SRR4302547.5049818/94‑1 (MQ=60)
                                        GCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCA                                                      >  SRR4302547.4753054/1‑94 (MQ=60)
                                        GCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCA                                                      <  SRR4302547.5328836/94‑1 (MQ=60)
                                         CGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAG                                                     >  SRR4302547.3724465/1‑94 (MQ=60)
                                          GTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGA                                                    >  SRR4302547.3365369/1‑94 (MQ=60)
                                           TCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGAC                                                   >  SRR4302547.4696457/1‑94 (MQ=60)
                                             AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                 <  SRR4302547.2098934/94‑1 (MQ=60)
                                             AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                 <  SRR4302547.2398540/94‑1 (MQ=60)
                                             AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                 >  SRR4302547.2913137/1‑94 (MQ=60)
                                             AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                 >  SRR4302547.115268/1‑94 (MQ=60)
                                                CAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTA                                              <  SRR4302547.1889468/94‑1 (MQ=60)
                                                 AATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTAC                                             <  SRR4302547.2741035/94‑1 (MQ=60)
                                                 AATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTAC                                             <  SRR4302547.534931/94‑1 (MQ=60)
                                                 AATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTAC                                             <  SRR4302547.4540416/94‑1 (MQ=60)
                                                  ATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTTATAAGCCAGACCTTTACC                                            >  SRR4302547.2613709/1‑94 (MQ=60)
                                                    TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                          <  SRR4302547.1030566/94‑1 (MQ=60)
                                                       GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCG                                       >  SRR4302547.179267/1‑94 (MQ=60)
                                                       GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCG                                       <  SRR4302547.613981/94‑1 (MQ=60)
                                                       GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCGTTACCTTTCG                                       >  SRR4302547.6028855/1‑94 (MQ=60)
                                                        GCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGA                                      >  SRR4302547.3055845/1‑94 (MQ=60)
                                                         CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                     <  SRR4302547.1740636/94‑1 (MQ=60)
                                                         CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                     <  SRR4302547.2476911/94‑1 (MQ=60)
                                                         CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCCGGTTCATAAGCCAGAGCTTTACCTTTCGAC                                     >  SRR4302547.3207098/1‑94 (MQ=60)
                                                           TGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAT                                   <  SRR4302547.4755487/94‑1 (MQ=60)
                                                            GAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATG                                  <  SRR4302547.6757660/94‑1 (MQ=60)
                                                            GAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATG                                  >  SRR4302547.3585610/1‑94 (MQ=60)
                                                               ATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCG                               <  SRR4302547.3629444/94‑1 (MQ=60)
                                                               ATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCG                               <  SRR4302547.3546386/94‑1 (MQ=60)
                                                                TCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGT                              <  SRR4302547.786070/94‑1 (MQ=60)
                                                                 CGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTA                             <  SRR4302547.4976734/94‑1 (MQ=60)
                                                                  GAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                            >  SRR4302547.6658480/1‑94 (MQ=60)
                                                                  GAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                            <  SRR4302547.2667572/94‑1 (MQ=60)
                                                                   AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                           >  SRR4302547.680748/1‑94 (MQ=60)
                                                                   AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                           >  SRR4302547.260363/1‑94 (MQ=60)
                                                                   AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                           <  SRR4302547.6665033/94‑1 (MQ=60)
                                                                    GGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGT                          >  SRR4302547.6582751/1‑94 (MQ=60)
                                                                    GGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGT                          <  SRR4302547.729042/94‑1 (MQ=60)
                                                                    GGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGT                          <  SRR4302547.3230325/94‑1 (MQ=60)
                                                                     GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                         <  SRR4302547.4825640/94‑1 (MQ=60)
                                                                     GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                         <  SRR4302547.4618898/94‑1 (MQ=60)
                                                                      TATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGG                        >  SRR4302547.1203344/1‑94 (MQ=60)
                                                                       ATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGT                       <  SRR4302547.3628625/94‑1 (MQ=60)
                                                                         TTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTT                     <  SRR4302547.106552/94‑1 (MQ=60)
                                                                         TTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGGTT                     >  SRR4302547.3872166/1‑94 (MQ=60)
                                                                           CTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTC                   >  SRR4302547.5843404/1‑94 (MQ=60)
                                                                             CCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAT                 <  SRR4302547.5405097/94‑1 (MQ=60)
                                                                              CGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATA                <  SRR4302547.1946892/94‑1 (MQ=60)
                                                                               GTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAC               <  SRR4302547.581607/94‑1 (MQ=60)
                                                                                TCGACCACAGCTGCTCTTCCCGCCACTGTCCTTTGGTTGCGGGTTCCTAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGCTTTCATACC              >  SRR4302547.6192325/1‑94 (MQ=60)
                                                                                 CGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCA             >  SRR4302547.5331609/1‑94 (MQ=60)
                                                                                  GACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAG            <  SRR4302547.1981037/94‑1 (MQ=60)
                                                                                   ACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGG           <  SRR4302547.5772553/94‑1 (MQ=60)
                                                                                   ACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGG           <  SRR4302547.5714654/94‑1 (MQ=60)
                                                                                     CACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGGTTTCATACCAGGGA         >  SRR4302547.6388364/1‑94 (MQ=60)
                                                                                      ACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAT        <  SRR4302547.2583380/94‑1 (MQ=60)
                                                                                       CAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATT       >  SRR4302547.3158068/1‑94 (MQ=60)
                                                                                            GCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCA  <  SRR4302547.2581577/94‑1 (MQ=60)
                                                                                            GCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCA  >  SRR4302547.2665600/1‑94 (MQ=60)
                                                                                             |                                                                                            
ATGCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCA  >  NC_000913/1652533‑1652718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑