Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,549,500 T→C T83A (ACG→GCG)  b2433 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,549,5000TC97.2% 598.3 / 3.4 180T83A (ACG→GCG) b2433hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (96/79);  total (99/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 193 total aligned reads displayed.

CCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACA  >  NC_000913/2549409‑2549589
                                                                                           |                                                                                         
ccGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTa                                                                                          >  1:2399878/1‑93 (MQ=255)
 cGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAg                                                                                         >  2:886918/1‑93 (MQ=255)
  gTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGc                                                                                        >  2:5320304/1‑93 (MQ=255)
   ttCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGcc                                                                                       >  1:1060109/1‑93 (MQ=255)
   ttCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGcc                                                                                       >  2:240540/1‑93 (MQ=255)
    tCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGccc                                                                                      <  2:4883054/93‑1 (MQ=255)
    tCAGCCACAGCCACGGTGGCCAGCAAAGTAAAACCACCGGCGCCAACAGCAACAGAAAAGAGAGTACACGCCAGGTGCGAGGAAACGCAGccc                                                                                      <  1:3056541/93‑1 (MQ=255)
      agccacagccacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCccgc                                                                                    <  1:2197696/93‑1 (MQ=255)
      agcaacagccacGGTTGCCAGCAAAGTAAACCCCCCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCccgc                                                                                    <  1:4928185/93‑1 (MQ=255)
       gccacagccacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCccgcc                                                                                   >  1:5269171/1‑93 (MQ=255)
         cacagccacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCccgccgc                                                                                 >  2:4865605/1‑92 (MQ=255)
           cagccacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGAcc                                                                               >  1:5068101/1‑93 (MQ=255)
           aggccacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGAcc                                                                               <  2:3441559/91‑1 (MQ=255)
            agccacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCg                                                                              >  2:3158365/1‑93 (MQ=255)
             gccacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGc                                                                             >  2:1950234/1‑93 (MQ=255)
               cacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTc                                                                           <  1:1913464/93‑1 (MQ=255)
               cacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTc                                                                           >  2:1754957/1‑93 (MQ=255)
                 cGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTcaa                                                                         >  2:1678784/1‑93 (MQ=255)
                  ggTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGCACACGCCAGGTGCGAGGAAACGTAGCACGGCGACCGCTcaac                                                                        >  1:1404082/1‑93 (MQ=255)
                   gTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTcaaca                                                                       >  2:5202412/1‑93 (MQ=255)
                    ttGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTcaacaa                                                                      >  1:2477725/1‑93 (MQ=255)
                       ccAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACaaaaa                                                                   <  2:4113385/93‑1 (MQ=255)
                        cAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAAc                                                                  >  2:4003466/1‑93 (MQ=255)
                        cAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAAc                                                                  <  2:2342913/93‑1 (MQ=255)
                           cAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGcc                                                               <  2:2194995/93‑1 (MQ=255)
                            aaaGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCa                                                              >  2:584591/1‑93 (MQ=255)
                            aaaGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGGACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCa                                                              >  1:3386351/1‑93 (MQ=255)
                             aaGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAg                                                             <  1:5716940/93‑1 (MQ=255)
                              aGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGc                                                            >  2:1983994/1‑93 (MQ=255)
                              aGTAAAACCACCTGCGCCAACAGCAACAGAAAAGAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGc                                                            <  2:2712546/93‑1 (MQ=255)
                               gTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCa                                                           >  1:258115/1‑93 (MQ=255)
                                tAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCAc                                                          <  1:5957210/93‑1 (MQ=255)
                                 aaaaCCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCAcc                                                         >  1:941152/1‑93 (MQ=255)
                                  aaaCCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCAccg                                                        <  1:4150945/93‑1 (MQ=255)
                                   aaCCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCAccgc                                                       >  1:2167496/1‑93 (MQ=255)
                                    accaccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCAccgcc                                                      <  2:1536698/93‑1 (MQ=255)
                                    accaccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTAAACAAAAACGCCAGCAccgcc                                                      >  1:2673233/1‑93 (MQ=255)
                                      caccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCgg                                                    >  2:593709/1‑93 (MQ=255)
                                       cccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGa                                                   <  2:4509660/92‑1 (MQ=255)
                                       cccTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGa                                                   <  1:3818695/92‑1 (MQ=255)
                                         cTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAAt                                                 >  1:237137/1‑93 (MQ=255)
                                         cTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAAt                                                 >  1:774442/1‑93 (MQ=255)
                                         cTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAAt                                                 <  2:2589717/93‑1 (MQ=255)
                                          tGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATg                                                <  1:281063/93‑1 (MQ=255)
                                           gcgcCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGc                                               <  2:5206064/93‑1 (MQ=255)
                                             gcCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAACACGCCAGCACCGCCGGAATGCCa                                             >  1:5855843/1‑93 (MQ=255)
                                             gcCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCa                                             >  2:5602115/1‑93 (MQ=255)
                                             cccAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCa                                             <  2:2455621/92‑1 (MQ=255)
                                               caacagcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAgg                                           >  2:1454157/1‑93 (MQ=255)
                                                 acagcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGaa                                         <  1:2931674/93‑1 (MQ=255)
                                                  cagcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAAt                                        <  1:4856565/93‑1 (MQ=255)
                                                   agcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATc                                       <  1:2474800/93‑1 (MQ=255)
                                                    gcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCa                                      >  2:1080569/1‑93 (MQ=255)
                                                    gcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCa                                      >  2:4275646/1‑93 (MQ=255)
                                                     caacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAg                                     >  1:4099601/1‑93 (MQ=255)
                                                      aacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGc                                    >  2:3188491/1‑93 (MQ=255)
                                                      aacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGc                                    >  1:1023120/1‑93 (MQ=255)
                                                      aacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGc                                    <  2:4088812/93‑1 (MQ=255)
                                                      aacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGc                                    <  2:920024/93‑1 (MQ=255)
                                                        cagAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGccc                                  >  2:1266049/1‑93 (MQ=255)
                                                         agAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGcccc                                 >  1:2964920/1‑93 (MQ=255)
                                                         agAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGcccc                                 >  1:4901761/1‑93 (MQ=255)
                                                          gAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCcca                                <  1:1102225/93‑1 (MQ=255)
                                                          gAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCcca                                <  2:2504956/93‑1 (MQ=255)
                                                           aaaaTAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCccag                               >  1:4601644/1‑93 (MQ=255)
                                                               tAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCccagccag                           >  1:4223281/1‑93 (MQ=255)
                                                               tAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCccagccag                           >  1:5698655/1‑93 (MQ=255)
                                                               tAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCccagccag                           >  1:5413002/1‑93 (MQ=255)
                                                               aagagTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCccagccag                           <  2:2241241/92‑1 (MQ=255)
                                                                 gagTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGaa                         <  2:95957/93‑1 (MQ=255)
                                                                   gTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAAt                       >  1:1831933/1‑93 (MQ=255)
                                                                    tACACGCCAGGTGCGAGTAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAAtt                      >  2:4169610/1‑93 (MQ=255)
                                                                    gccacGCGAGCTGCGAGGAACCGCAGCCCGCCGCCCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAAtt                      <  1:3499465/91‑1 (MQ=255)
                                                                     acacGCCAGGTGCGAGGAACCGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTa                     <  1:1628751/93‑1 (MQ=255)
                                                                      cacGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTAt                    <  2:6052449/93‑1 (MQ=255)
                                                                      cacGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTAt                    <  2:2209638/93‑1 (MQ=255)
                                                                       ccGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATc                   <  2:442104/92‑1 (MQ=255)
                                                                       acGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATc                   <  2:2576695/93‑1 (MQ=255)
                                                                        cGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCg                  <  1:3432568/93‑1 (MQ=255)
                                                                         gCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGt                 <  2:273479/93‑1 (MQ=255)
                                                                         gCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACAGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGt                 >  2:4632715/1‑93 (MQ=255)
                                                                          ccAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTg                >  2:3026396/1‑93 (MQ=255)
                                                                          ccAGGTGCGAGAAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTg                <  2:6126017/93‑1 (MQ=255)
                                                                            aGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGAt              <  2:3868157/93‑1 (MQ=255)
                                                                            aGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGAt              <  2:3220379/93‑1 (MQ=255)
                                                                             ggTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGAtg             >  1:957229/1‑92 (MQ=255)
                                                                               tGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCgg           <  1:5530714/93‑1 (MQ=255)
                                                                                 cGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGa         <  1:5717011/93‑1 (MQ=255)
                                                                                  gAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGAt        >  2:5079090/1‑93 (MQ=255)
                                                                                   aGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATa       <  1:1348564/93‑1 (MQ=255)
                                                                                   aGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATa       >  2:5957102/1‑93 (MQ=255)
                                                                                    ggAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATaa      >  1:1997775/1‑93 (MQ=255)
                                                                                    ggAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATaa      <  2:367737/93‑1 (MQ=255)
                                                                                    ggAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATaa      >  2:2775362/1‑93 (MQ=255)
                                                                                     gAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATaaa     >  2:228695/1‑93 (MQ=255)
                                                                                     gAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATaaa     <  1:4518326/93‑1 (MQ=255)
                                                                                      aaaCGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATaaaa    <  1:3832403/93‑1 (MQ=255)
                                                                                       aaCGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAAc   <  1:1963077/93‑1 (MQ=255)
                                                                                       aaCGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAAc   <  1:3627181/93‑1 (MQ=255)
                                                                                        aCGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACa  >  1:3927207/1‑93 (MQ=255)
                                                                                           |                                                                                         
CCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACA  >  NC_000913/2549409‑2549589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATT  >  NC_000913/2549407‑2549593
                                                                                             |                                                                                             
CACCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGC                                                                                               >  SRR4302532.3106122/1‑94 (MQ=60)
 ACCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTA                                                                                              >  SRR4302532.2479779/1‑94 (MQ=60)
  CCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGTGAGGAAACGCAG                                                                                             <  SRR4302532.1442748/94‑1 (MQ=60)
    GTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCC                                                                                           >  SRR4302532.1095818/1‑94 (MQ=60)
      TCAGCCACAGCCACGGTGGCCAGCAAAGTAAAACCACCGGCGCCAACAGCAACAGAAAAGAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCG                                                                                         <  SRR4302532.3157459/94‑1 (MQ=60)
        AGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCC                                                                                       >  SRR4302532.5450863/1‑94 (MQ=60)
        AGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCC                                                                                       <  SRR4302532.2270877/94‑1 (MQ=60)
        AGCAACAGCCACGGTTGCCAGCAAAGTAAACCCCCCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCC                                                                                       <  SRR4302532.5097422/94‑1 (MQ=60)
         GCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCG                                                                                      >  SRR4302532.3260621/1‑94 (MQ=60)
          CCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGA                                                                                     >  SRR4302532.4364709/1‑94 (MQ=60)
          CCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGA                                                                                     >  SRR4302532.1921263/1‑94 (MQ=60)
           CACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGAC                                                                                    >  SRR4302532.5612558/1‑94 (MQ=60)
            ACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACC                                                                                   >  SRR4302532.5242222/1‑94 (MQ=60)
                CCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTC                                                                               >  SRR4302532.3964801/1‑94 (MQ=60)
                 CACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCA                                                                              <  SRR4302532.1977063/94‑1 (MQ=60)
                 CACGGTGGCAAGCAAAGAAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCA                                                                              <  SRR4302532.4776359/94‑1 (MQ=60)
                  ACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAA                                                                             >  SRR4302532.6151679/1‑94 (MQ=60)
                   CGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGCACACGCCAGGTGCGAGGAAACGTAGCACGGCGACCGCTCAAC                                                                            >  SRR4302532.1451110/1‑94 (MQ=60)
                     GTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAA                                                                          >  SRR4302532.2560270/1‑94 (MQ=60)
                         caaaaaaagaaaaacctcccgcgccaagagaaaaAGAAAATAGATTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAAC                                                                      <  SRR4302532.2521668/60‑1 (MQ=60)
                          CAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACG                                                                     <  SRR4302532.1987529/94‑1 (MQ=60)
                           AGCAAAGTAAACCCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGC                                                                    <  SRR4302532.396516/94‑1 (MQ=60)
                             CAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCA                                                                  >  SRR4302532.2136437/1‑94 (MQ=60)
                             CAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCA                                                                  <  SRR4302532.5093143/94‑1 (MQ=60)
                             CAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGGACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCA                                                                  >  SRR4302532.3499609/1‑94 (MQ=60)
                               AAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGC                                                                >  SRR4302532.2688014/1‑94 (MQ=60)
                               AAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGC                                                                <  SRR4302532.5916115/94‑1 (MQ=60)
                               AAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGC                                                                >  SRR4302532.3142960/1‑94 (MQ=60)
                                AGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCA                                                               >  SRR4302532.267070/1‑94 (MQ=60)
                                 GTAAAACCACCTGCGCCAACAGCAACAGAAAATAGGGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCAC                                                              >  SRR4302532.6196376/1‑94 (MQ=60)
                                  TAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACC                                                             >  SRR4302532.973405/1‑94 (MQ=60)
                                  TAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACC                                                             >  SRR4302532.973404/1‑94 (MQ=60)
                                  TAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACC                                                             <  SRR4302532.6163259/94‑1 (MQ=60)
                                   aaaaccaccgccacaaacaacaaaaaAAAATAGAGCACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCG                                                            <  SRR4302532.1310340/68‑1 (MQ=60)
                                   AAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCG                                                            >  SRR4302532.4288315/1‑94 (MQ=60)
                                    AAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGC                                                           <  SRR4302532.4290197/94‑1 (MQ=60)
                                    AAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGC                                                           >  SRR4302532.2239987/1‑94 (MQ=60)
                                     AACCACCTGCGCCAACAGCAACAGAAAATAGATTCCACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCC                                                          <  SRR4302532.3826841/94‑1 (MQ=60)
                                     AACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTAAACAAAAACGCCAGCACCGCC                                                          >  SRR4302532.2762958/1‑94 (MQ=60)
                                      ACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCG                                                         >  SRR4302532.4665704/1‑94 (MQ=60)
                                         CCCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAA                                                      <  SRR4302532.3947611/94‑1 (MQ=60)
                                         ACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAA                                                      >  SRR4302532.4734180/1‑94 (MQ=60)
                                          CCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAAT                                                     >  SRR4302532.801124/1‑94 (MQ=60)
                                          CCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAAT                                                     >  SRR4302532.245294/1‑94 (MQ=60)
                                            TGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGC                                                   <  SRR4302532.290785/94‑1 (MQ=60)
                                              CGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAACACGCCAGCACCGCCGGAATGCCA                                                 >  SRR4302532.6058909/1‑94 (MQ=60)
                                                 CAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGA                                              >  SRR4302532.374310/1‑94 (MQ=60)
                                                   ACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAAT                                            <  SRR4302532.3029144/94‑1 (MQ=60)
                                                   ACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAAT                                            <  SRR4302532.5960308/94‑1 (MQ=60)
                                                    CAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATC                                           <  SRR4302532.5023667/94‑1 (MQ=60)
                                                    CAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATC                                           <  SRR4302532.2241179/94‑1 (MQ=60)
                                                    CAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATC                                           >  SRR4302532.5531419/1‑94 (MQ=60)
                                                     accaaaaaaaaaggaagaacgCGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCCCCGCCGGAATGCCAGGAATCA                                          <  SRR4302532.2525219/73‑1 (MQ=60)
                                                     AGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCA                                          <  SRR4302532.2557219/94‑1 (MQ=60)
                                                      GCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCAGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAG                                         <  SRR4302532.1027721/94‑1 (MQ=60)
                                                      GCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAG                                         >  SRR4302532.4237214/1‑94 (MQ=60)
                                                      GCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAG                                         >  SRR4302532.809911/1‑94 (MQ=60)
                                                       CAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGC                                        <  SRR4302532.5193181/94‑1 (MQ=60)
                                                       CAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGC                                        >  SRR4302532.1057768/1‑94 (MQ=60)
                                                        aaaaaAAAATAGAGTCCACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCC                                       <  SRR4302532.2459865/89‑1 (MQ=60)
                                                         ACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCC                                      >  SRR4302532.1845743/1‑94 (MQ=60)
                                                          CAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCC                                     >  SRR4302532.3063117/1‑94 (MQ=60)
                                                          CAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCC                                     >  SRR4302532.5070206/1‑94 (MQ=60)
                                                           AGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCA                                    >  SRR4302532.254933/1‑94 (MQ=60)
                                                           AGAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCA                                    >  SRR4302532.2456451/1‑94 (MQ=60)
                                                            GAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAG                                   >  SRR4302532.4758532/1‑94 (MQ=60)
                                                            GAAAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAG                                   <  SRR4302532.1139080/94‑1 (MQ=60)
                                                              AAATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCC                                 >  SRR4302532.537462/1‑94 (MQ=60)
                                                                ATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAG                               >  SRR4302532.4365009/1‑94 (MQ=60)
                                                                ATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAG                               >  SRR4302532.5897433/1‑94 (MQ=60)
                                                                ATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAG                               >  SRR4302532.5600553/1‑94 (MQ=60)
                                                                ATAGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAG                               >  SRR4302532.5897432/1‑94 (MQ=60)
                                                                AGAGAGTACACGCCAGGTGCAAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAG                               <  SRR4302532.3525046/94‑1 (MQ=60)
                                                                  AGAGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAA                             >  SRR4302532.3615804/1‑94 (MQ=60)
                                                                    acaACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAAT                           <  SRR4302532.1446473/91‑1 (MQ=60)
                                                                    AGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAAT                           >  SRR4302532.5057399/1‑94 (MQ=60)
                                                                    AGTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAAT                           >  SRR4302532.1892870/1‑94 (MQ=60)
                                                                     GTACACGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATT                          >  SRR4302532.5736500/1‑94 (MQ=60)
                                                                      gccacgcgagcTGCGAGGAACCGCAGCCCGCCGCCCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTA                         <  SRR4302532.3616700/83‑1 (MQ=60)
                                                                       ACATGCCGGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTAT                        <  SRR4302532.3036589/94‑1 (MQ=60)
                                                                       ACACGCCAGGTGCGAGGAACCGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTAT                        <  SRR4302532.1683885/94‑1 (MQ=60)
                                                                          CGCCAGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGT                     <  SRR4302532.3547575/94‑1 (MQ=60)
                                                                              AGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATG                 >  SRR4302532.989909/1‑94 (MQ=60)
                                                                              AGGTGCGAGGAAACGCAGCCCGCCGACCGCTCAAAAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATG                 >  SRR4302532.2989264/1‑94 (MQ=60)
                                                                                 TGCGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGG              <  SRR4302532.5722642/94‑1 (MQ=60)
                                                                                   CGAGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGAT            <  SRR4302532.5916186/94‑1 (MQ=60)
                                                                                    GACGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATA           <  SRR4302532.2040247/94‑1 (MQ=60)
                                                                                    CAGGAAACGCACCCCGCCGCCCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATA           <  SRR4302532.1504891/94‑1 (MQ=60)
                                                                                     AGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAA          <  SRR4302532.1393653/94‑1 (MQ=60)
                                                                                     AGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAA          >  SRR4302532.1111541/1‑94 (MQ=60)
                                                                                     AGGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAA          >  SRR4302532.2064336/1‑94 (MQ=60)
                                                                                      GGAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAA         <  SRR4302532.2640893/94‑1 (MQ=60)
                                                                                       GAAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAA        <  SRR4302532.4672572/94‑1 (MQ=60)
                                                                                        AAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAAC       >  SRR4302532.2306976/1‑94 (MQ=60)
                                                                                        AAACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAAC       <  SRR4302532.3961791/94‑1 (MQ=60)
                                                                                         AACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACA      <  SRR4302532.2028272/94‑1 (MQ=60)
                                                                                         AACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACA      <  SRR4302532.3748798/94‑1 (MQ=60)
                                                                                         AACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACA      >  SRR4302532.4059522/1‑94 (MQ=60)
                                                                                          ACGCAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCTGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAG     <  SRR4302532.2845458/94‑1 (MQ=60)
                                                                                            GCAGCCCGCCGACCGCTCAACAAGAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGAT   <  SRR4302532.3619488/94‑1 (MQ=60)
                                                                                             CAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATT  >  SRR4302532.5279435/1‑94 (MQ=60)
                                                                                             CAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATT  >  SRR4302532.4369010/1‑94 (MQ=60)
                                                                                             |                                                                                             
CACCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATT  >  NC_000913/2549407‑2549593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑