Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,781,027 C→G intergenic (‑151/+58) ypjA ← / ← b2648 putative ATP‑binding component of a transport system/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,781,0270CG99.6% 812.8 / NA 244intergenic (‑151/+58)ypjA/b2648putative ATP‑binding component of a transport system/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (106/136);  minor base A (0/1);  total (106/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

AACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTT  >  NC_000913/2780938‑2781116
                                                                                          |                                                                                         
aaCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCa                                                                                         <  1:4453412/93‑1 (MQ=255)
   gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                                      >  1:668714/1‑93 (MQ=255)
      gTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataa                                                                                   <  1:168305/93‑1 (MQ=25)
       tGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaa                                                                                  >  1:1254953/1‑93 (MQ=255)
        gcgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaa                                                                                 <  2:1267023/93‑1 (MQ=25)
        gcgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaa                                                                                 <  1:3935552/93‑1 (MQ=25)
         cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                                >  2:1739667/1‑93 (MQ=255)
         cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                                >  2:2742533/1‑93 (MQ=255)
          gcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATg                                                                               >  1:1890588/1‑93 (MQ=255)
           cTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                                              <  2:6020269/93‑1 (MQ=25)
           cTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                                              <  1:1482423/93‑1 (MQ=25)
            tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                             <  1:892280/93‑1 (MQ=255)
            tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                             >  2:4040921/1‑93 (MQ=255)
             gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGCAATACGGGCGTATTCTTTTCTTTCGACAATAAGGCCGTTGGCAAAATACAATGagt                                                                            >  2:4704759/1‑91 (MQ=255)
             gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                            >  1:2343150/1‑93 (MQ=255)
             gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                            >  1:4039387/1‑93 (MQ=255)
              aaGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAttt                                                                           <  2:42028/93‑1 (MQ=37)
               aGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTa                                                                          <  2:5544659/93‑1 (MQ=37)
                gtgtGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAc                                                                         <  1:3758282/93‑1 (MQ=37)
                 tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACa                                                                        >  1:2720686/1‑93 (MQ=255)
                 tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACa                                                                        <  2:706849/93‑1 (MQ=255)
                   tgGTTGGATAACAAAGTCCATCCATTCAGGCAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCACAATCAAATGATTTACATa                                                                      >  2:12820/1‑93 (MQ=25)
                   tgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATa                                                                      <  2:3791306/93‑1 (MQ=37)
                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    >  1:5785345/1‑93 (MQ=255)
                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    <  2:4435044/93‑1 (MQ=255)
                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    >  2:4553265/1‑93 (MQ=255)
                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    <  1:5191718/93‑1 (MQ=37)
                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    <  2:2413455/93‑1 (MQ=255)
                      ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                                   <  2:4726526/93‑1 (MQ=255)
                      ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                                   >  1:4834143/1‑93 (MQ=255)
                      ttGGATAAAAAAGGCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                                   <  1:5272021/93‑1 (MQ=37)
                         gATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGtt                                                                <  1:2212920/93‑1 (MQ=37)
                         gATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGtt                                                                <  1:4478809/93‑1 (MQ=37)
                                aaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatg                                                         >  2:5269974/1‑93 (MQ=255)
                                 aGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatga                                                        <  1:5835389/93‑1 (MQ=255)
                                  gTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                                                       <  1:1085952/93‑1 (MQ=255)
                                  gTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                                                       <  1:1778482/93‑1 (MQ=255)
                                   tCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAat                                                      <  1:4740006/93‑1 (MQ=37)
                                     aaTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAatat                                                    >  2:4355561/1‑93 (MQ=255)
                                      aTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtc                                                   <  2:1082932/93‑1 (MQ=25)
                                      aTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtc                                                   >  2:1487056/1‑93 (MQ=255)
                                       tCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCNACAAAGAGGCCGTTGGCAAAATAAAATGATTTANATAATCGTTTCTGATGAatatat                                                  >  2:2658865/1‑91 (MQ=21)
                                       accATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtct                                                  <  1:4895237/92‑1 (MQ=21)
                                        ccATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtctt                                                 <  2:5769366/93‑1 (MQ=25)
                                         cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                                <  1:1569604/93‑1 (MQ=25)
                                         cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                                <  1:3422250/93‑1 (MQ=255)
                                          aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                               >  1:3353707/1‑93 (MQ=255)
                                            tCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGc                                             >  2:2576211/1‑93 (MQ=255)
                                            tCAGGAAATACGGGCGCATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGc                                             <  1:4910685/93‑1 (MQ=21)
                                               tgAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                          >  2:1933258/2‑93 (MQ=21)
                                               ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                          <  2:3917342/93‑1 (MQ=255)
                                                gAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTcac                                         >  2:638973/1‑93 (MQ=255)
                                                gAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTcac                                         >  1:2095137/1‑93 (MQ=255)
                                                 aaaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTGGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTcaca                                        <  1:537472/93‑1 (MQ=18)
                                                  aaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACAt                                       <  2:2878388/93‑1 (MQ=18)
                                                  aaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATAAATATCTTCTGCTCACAt                                       >  1:611605/1‑93 (MQ=18)
                                                        ggCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAt                                 >  2:4180226/1‑93 (MQ=18)
                                                          cGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATca                               <  2:769875/93‑1 (MQ=255)
                                                            tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                             >  1:2917230/1‑93 (MQ=255)
                                                            tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                             >  1:4495732/1‑93 (MQ=18)
                                                             aTTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacac                            <  2:856990/93‑1 (MQ=255)
                                                             aTTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacac                            >  2:4501290/1‑93 (MQ=17)
                                                              ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                           >  1:1713939/1‑93 (MQ=255)
                                                              ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                           <  1:1394915/93‑1 (MQ=17)
                                                               tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACaa                          <  1:789953/93‑1 (MQ=17)
                                                               tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATACCACaa                          >  1:1917698/1‑93 (MQ=14)
                                                                cttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATAACACAAt                         >  2:1548972/1‑93 (MQ=17)
                                                                 ttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATa                        >  2:493278/1‑93 (MQ=14)
                                                                 ttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATa                        <  2:4598862/93‑1 (MQ=255)
                                                                  tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa                       >  2:355313/1‑93 (MQ=17)
                                                                  tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa                       <  1:5138188/93‑1 (MQ=255)
                                                                  tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa                       <  1:2478549/93‑1 (MQ=255)
                                                                    tctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACt                     <  2:339569/93‑1 (MQ=255)
                                                                    tctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACt                     >  1:5222936/1‑93 (MQ=14)
                                                                       ttcGACAATGAGGCCGTTGACAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTg                  <  2:5987793/93‑1 (MQ=255)
                                                                        tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga                 <  2:848897/93‑1 (MQ=255)
                                                                        tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga                 <  2:437104/93‑1 (MQ=255)
                                                                        tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga                 <  2:2655960/93‑1 (MQ=255)
                                                                          gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga               <  2:4114923/93‑1 (MQ=255)
                                                                          gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga               <  1:3528869/93‑1 (MQ=255)
                                                                          gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga               <  1:5332293/93‑1 (MQ=255)
                                                                           aCAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAt              <  2:4123977/93‑1 (MQ=255)
                                                                           aCAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAt              <  2:6043712/93‑1 (MQ=255)
                                                                            cAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATc             <  2:5032083/93‑1 (MQ=255)
                                                                             aaTGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCg            >  2:3055117/1‑93 (MQ=18)
                                                                               tgaggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGTATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCa          <  1:3274830/89‑1 (MQ=18)
                                                                               tgaggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAATATCACACAATAACTTTGAGATCGCa          >  1:4589822/5‑93 (MQ=18)
                                                                               tgaggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCa          >  2:2227581/5‑93 (MQ=255)
                                                                               tgaggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCa          >  2:4311481/5‑93 (MQ=255)
                                                                                gaggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAg         <  1:5108700/90‑1 (MQ=255)
                                                                                 aggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGa        <  2:2176135/91‑1 (MQ=255)
                                                                                   gCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAtt      >  2:4849880/1‑93 (MQ=255)
                                                                                   gCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAtt      <  2:1044662/93‑1 (MQ=255)
                                                                                    ccGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTg     <  2:3140282/93‑1 (MQ=255)
                                                                                     cGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTCAGATCGCAGATTGt    >  1:5517488/1‑93 (MQ=255)
                                                                                      gTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGtt   <  1:5445592/93‑1 (MQ=255)
                                                                                      gTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGtt   <  2:1847568/93‑1 (MQ=255)
                                                                                       ttGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGttt  >  2:2960435/1‑93 (MQ=255)
                                                                                       ttGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGttt  <  2:5139307/93‑1 (MQ=255)
                                                                                       ttGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGttt  >  2:3281444/1‑93 (MQ=255)
                                                                                          |                                                                                         
AACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTT  >  NC_000913/2780938‑2781116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACT  >  NC_000913/2780936‑2781120
                                                                                            |                                                                                             
TAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGC                                                                                              <  SRR4302532.520430/94‑1 (MQ=60)
TAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGC                                                                                              <  SRR4302532.4919305/94‑1 (MQ=60)
TAAACGCAGTGCGCTGAAGTGTGATTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGGCAATGAGGCCGTTGGC                                                                                              <  SRR4302532.1544667/94‑1 (MQ=60)
 AAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCA                                                                                             >  SRR4302532.2026059/1‑94 (MQ=60)
 AAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCA                                                                                             >  SRR4302532.6187754/1‑94 (MQ=60)
  AACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAA                                                                                            <  SRR4302532.4605195/94‑1 (MQ=60)
    CGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAA                                                                                          >  SRR4302532.691785/1‑94 (MQ=60)
    CGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAA                                                                                          >  SRR4302532.644805/1‑94 (MQ=60)
      CAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATA                                                                                        >  SRR4302532.6314597/1‑94 (MQ=60)
        GTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAA                                                                                      <  SRR4302532.5298965/94‑1 (MQ=60)
        GTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAA                                                                                      >  SRR4302532.1296944/1‑94 (MQ=60)
        GTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAA                                                                                      <  SRR4302532.173944/94‑1 (MQ=60)
          GCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAAT                                                                                    <  SRR4302532.4068158/94‑1 (MQ=60)
           CGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATG                                                                                   >  SRR4302532.1953277/1‑94 (MQ=60)
            GCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGA                                                                                  >  SRR4302532.491156/1‑94 (MQ=60)
             CTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAT                                                                                 <  SRR4302532.1532405/94‑1 (MQ=60)
              TGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATT                                                                                >  SRR4302532.2421258/1‑94 (MQ=60)
              TGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATT                                                                                >  SRR4302532.4175460/1‑94 (MQ=60)
              TGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATT                                                                                <  SRR4302532.922749/94‑1 (MQ=60)
               GAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTT                                                                               <  SRR4302532.2896214/94‑1 (MQ=60)
                  GTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACA                                                                            >  SRR4302532.2812189/1‑94 (MQ=60)
                  GTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACA                                                                            <  SRR4302532.3884710/94‑1 (MQ=60)
                  GTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACA                                                                            <  SRR4302532.5504871/94‑1 (MQ=60)
                    GTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATA                                                                          >  SRR4302532.1563983/1‑94 (MQ=60)
                    GTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATA                                                                          >  SRR4302532.2174777/1‑94 (MQ=60)
                    GTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATA                                                                          >  SRR4302532.1249214/1‑94 (MQ=60)
                     TGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAA                                                                         >  SRR4302532.1830136/1‑94 (MQ=60)
                      GGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAT                                                                        <  SRR4302532.4650041/94‑1 (MQ=60)
                      GGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAT                                                                        <  SRR4302532.2605814/94‑1 (MQ=60)
                      GGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAT                                                                        >  SRR4302532.5986540/1‑94 (MQ=60)
                       GTTGTATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATC                                                                       <  SRR4302532.3349353/94‑1 (MQ=60)
                       GTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATC                                                                       <  SRR4302532.5370653/94‑1 (MQ=60)
                       GTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATC                                                                       >  SRR4302532.5000540/1‑94 (MQ=60)
                        TTGGATAAAAAAGGCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCG                                                                      <  SRR4302532.5453817/94‑1 (MQ=60)
                           GATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTT                                                                   <  SRR4302532.4631612/94‑1 (MQ=60)
                           GATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTT                                                                   <  SRR4302532.2286447/94‑1 (MQ=60)
                              AAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTG                                                                <  SRR4302532.740900/94‑1 (MQ=60)
                              AAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTG                                                                <  SRR4302532.4867209/94‑1 (MQ=60)
                              AAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTG                                                                >  SRR4302532.1963890/1‑94 (MQ=60)
                                AAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGAT                                                              >  SRR4302532.1454106/1‑94 (MQ=60)
                                 AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATCAAATGATTTACATAATCGTTTCTGATG                                                             >  SRR4302532.4615888/1‑94 (MQ=60)
                                 AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATG                                                             <  SRR4302532.5075493/94‑1 (MQ=60)
                                 AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATG                                                             <  SRR4302532.5921157/94‑1 (MQ=60)
                                 AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATG                                                             <  SRR4302532.1520881/94‑1 (MQ=60)
                                   AGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAA                                                           <  SRR4302532.6037965/94‑1 (MQ=60)
                                    GTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAAT                                                          <  SRR4302532.1122332/94‑1 (MQ=60)
                                    GTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAAT                                                          <  SRR4302532.342767/94‑1 (MQ=60)
                                    GTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAAT                                                          <  SRR4302532.1837991/94‑1 (MQ=60)
                                     TCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATA                                                         <  SRR4302532.4623325/94‑1 (MQ=60)
                                     TCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATA                                                         <  SRR4302532.5430959/94‑1 (MQ=60)
                                     TCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATA                                                         <  SRR4302532.4901861/94‑1 (MQ=60)
                                     TCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATA                                                         <  SRR4302532.2211579/94‑1 (MQ=60)
                                      CAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAT                                                        <  SRR4302532.4567037/94‑1 (MQ=60)
                                       AATCCATTCAGGAAATACGGGCGTATTCTTTTCTTGCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATC                                                       >  SRR4302532.4109803/1‑94 (MQ=60)
                                         TCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTT                                                     >  SRR4302532.4771322/1‑94 (MQ=60)
                                         TCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTT                                                     <  SRR4302532.2420693/94‑1 (MQ=60)
                                         ACCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTT                                                     <  SRR4302532.5063470/94‑1 (MQ=60)
                                           CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                                   <  SRR4302532.931631/94‑1 (MQ=60)
                                           CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                                   <  SRR4302532.1767224/94‑1 (MQ=60)
                                           CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                                   <  SRR4302532.1622720/94‑1 (MQ=60)
                                           CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                                   <  SRR4302532.3536894/94‑1 (MQ=60)
                                           CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                                   >  SRR4302532.3465671/1‑94 (MQ=60)
                                            ATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTG                                                  >  SRR4302532.3815952/1‑94 (MQ=60)
                                            ATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTG                                                  <  SRR4302532.1831792/94‑1 (MQ=60)
                                              TCAGGAAATACGGGCGCATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCT                                                <  SRR4302532.5079417/94‑1 (MQ=60)
                                               CAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTA                                               >  SRR4302532.353866/1‑94 (MQ=60)
                                                TGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAGATACTTAATCGTTTCTGATGAATAGCTTCTGCTCT                                              >  SRR4302532.5683777/1‑94 (MQ=52)
                                                 GGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCAC                                             >  SRR4302532.2165025/1‑94 (MQ=60)
                                                  GAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACA                                            >  SRR4302532.166138/1‑94 (MQ=60)
                                                   AAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACAT                                           <  SRR4302532.556198/94‑1 (MQ=60)
                                                   AAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACAT                                           <  SRR4302532.392388/94‑1 (MQ=60)
                                                   AAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATAAATATCTTCTGCTCACAT                                           >  SRR4302532.632924/1‑94 (MQ=60)
                                                   AAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTGGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACAT                                           <  SRR4302532.556196/94‑1 (MQ=60)
                                                       ACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAA                                       >  SRR4302532.5443133/1‑94 (MQ=60)
                                                         GGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAT                                     <  SRR4302532.3669352/94‑1 (MQ=60)
                                                         GGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAT                                     >  SRR4302532.590255/1‑94 (MQ=60)
                                                         CGGCGTATTCTTTTCTTTCGACAATGATGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAT                                     <  SRR4302532.5525719/94‑1 (MQ=60)
                                                           GCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCA                                   >  SRR4302532.139077/1‑94 (MQ=60)
                                                           GCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCA                                   <  SRR4302532.776282/94‑1 (MQ=60)
                                                            CGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCAC                                  <  SRR4302532.3131552/94‑1 (MQ=60)
                                                            CGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCAC                                  >  SRR4302532.601554/1‑94 (MQ=60)
                                                            CGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCAC                                  <  SRR4302532.4164301/94‑1 (MQ=60)
                                                             GTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACA                                 <  SRR4302532.5548661/94‑1 (MQ=60)
                                                             GTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACA                                 >  SRR4302532.3014425/1‑94 (MQ=60)
                                                             GTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACA                                 >  SRR4302532.4649212/1‑94 (MQ=60)
                                                               ATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACA                               >  SRR4302532.1771712/1‑94 (MQ=56)
                                                                TTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTTCATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAA                              >  SRR4302532.1981473/1‑94 (MQ=52)
                                                                TTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAA                              <  SRR4302532.1441688/94‑1 (MQ=54)
                                                                TTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATACCACAA                              >  SRR4302532.1981474/1‑94 (MQ=51)
                                                                 TCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAT                             >  SRR4302532.4646255/1‑94 (MQ=52)
                                                                 TCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAT                             <  SRR4302532.5338827/94‑1 (MQ=52)
                                                                 TCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAT                             <  SRR4302532.817026/94‑1 (MQ=52)
                                                                   TTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAA                           <  SRR4302532.1695906/94‑1 (MQ=47)
                                                                   TTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAA                           >  SRR4302532.1012138/1‑94 (MQ=47)
                                                                   TTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAA                           <  SRR4302532.2758435/94‑1 (MQ=47)
                                                                    TTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAC                          <  SRR4302532.3578357/94‑1 (MQ=47)
                                                                    TTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAC                          <  SRR4302532.2561126/94‑1 (MQ=47)
                                                                    TTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAC                          <  SRR4302532.5314954/94‑1 (MQ=47)
                                                                     TTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACT                         >  SRR4302532.5403053/1‑94 (MQ=47)
                                                                      TCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTT                        >  SRR4302532.5371921/1‑94 (MQ=47)
                                                                         TTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGA                     <  SRR4302532.3524167/94‑1 (MQ=54)
                                                                          TCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAG                    <  SRR4302532.5240062/94‑1 (MQ=56)
                                                                          TCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAG                    <  SRR4302532.1863694/94‑1 (MQ=56)
                                                                            GACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAT                  <  SRR4302532.5517203/94‑1 (MQ=60)
                                                                            GACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAT                  <  SRR4302532.3646986/94‑1 (MQ=60)
                                                                               AATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGC               <  SRR4302532.2434884/94‑1 (MQ=60)
                                                                                ATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAATATCACACAATAACTTTGAGATCGCA              >  SRR4302532.4746305/1‑94 (MQ=60)
                                                                                ATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCA              <  SRR4302532.1345631/94‑1 (MQ=60)
                                                                                ATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCA              <  SRR4302532.191067/94‑1 (MQ=60)
                                                                                 TGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGTATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAG             <  SRR4302532.3383736/94‑1 (MQ=60)
                                                                                  gagGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGA            <  SRR4302532.5348009/91‑1 (MQ=60)
                                                                                  gagGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGA            <  SRR4302532.5284328/91‑1 (MQ=60)
                                                                                   agGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAT           >  SRR4302532.504825/3‑94 (MQ=60)
                                                                                      CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTCAGATCGCAGATTGT        >  SRR4302532.5708942/1‑94 (MQ=60)
                                                                                       CGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACACTAACTTTGAGATCGCAGATTGTT       >  SRR4302532.5042819/1‑94 (MQ=60)
                                                                                       CGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTT       >  SRR4302532.870175/1‑94 (MQ=60)
                                                                                       CGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTT       <  SRR4302532.5089199/94‑1 (MQ=60)
                                                                                       CGTGGGCAAAATAAAATCATTTACATAATCGTTTCTGATGAATATCTTCTGGTCACATAAAAATCACACAATAACTCTGAGATCTCAGATTGGT       <  SRR4302532.2641526/94‑1 (MQ=55)
                                                                                        GTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTT      <  SRR4302532.5634330/94‑1 (MQ=60)
                                                                                         TTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTT     >  SRR4302532.5067408/1‑94 (MQ=60)
                                                                                          TGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTA    >  SRR4302532.3231113/1‑94 (MQ=60)
                                                                                          TGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTA    >  SRR4302532.3954503/1‑94 (MQ=60)
                                                                                           GGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTAC   <  SRR4302532.1175619/94‑1 (MQ=60)
                                                                                            GCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACT  >  SRR4302532.5566563/1‑94 (MQ=60)
                                                                                            GCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACATTGAGATCGCAGATTGTTTTACT  >  SRR4302532.3611322/1‑94 (MQ=60)
                                                                                            |                                                                                             
TAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACT  >  NC_000913/2780936‑2781120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑