Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,025,676 +C coding (535/570 nt) ygfQ → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,025,6751.C98.9% 637.1 / ‑5.0 179L178F (TTG→TTC) ygfQhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  major base C (103/72);  minor base A (0/2);  total (103/76)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG  >  NC_000913/3025583‑3025762
                                                                                             |                                                                                       
gCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTg                                                                                          >  2:4697571/1‑93 (MQ=255)
gCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTg                                                                                          <  2:4254506/93‑1 (MQ=255)
 cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑c                                                                                        <  1:1694955/93‑1 (MQ=255)
 cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑c                                                                                        >  1:4008054/1‑93 (MQ=255)
 cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑c                                                                                        >  1:384852/1‑93 (MQ=255)
  tCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cc                                                                                       <  2:2524747/93‑2 (MQ=255)
   cACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cca                                                                                      <  1:4354517/93‑3 (MQ=255)
    aCGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑ccag                                                                                     >  1:2319337/1‑90 (MQ=255)
       gTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                   <  1:2878420/93‑1 (MQ=255)
         aCAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGgcg                                                                                 >  1:5744483/1‑93 (MQ=255)
          cAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGgcgc                                                                                >  2:2503931/1‑93 (MQ=255)
             gtatcggtatcgGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCg                                                                             >  1:4119041/1‑93 (MQ=255)
                tcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTg                                                                          >  2:184531/1‑93 (MQ=255)
                tcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGATATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTg                                                                          >  2:3231934/1‑93 (MQ=255)
                    tatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGtt                                                                      >  1:5868577/1‑93 (MQ=255)
                     atcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGttt                                                                     <  2:768183/93‑1 (MQ=255)
                      tcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTa                                                                    >  2:4896004/1‑93 (MQ=255)
                        gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                  >  2:3425567/1‑93 (MQ=255)
                        gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                  >  2:4087552/1‑93 (MQ=255)
                         ggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcct                                                                 >  1:2555342/1‑93 (MQ=255)
                          gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctc                                                                <  2:1250830/93‑1 (MQ=255)
                          gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctc                                                                >  2:3178923/1‑93 (MQ=255)
                           gtGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcc                                                               <  2:1566111/92‑1 (MQ=255)
                           cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcc                                                               >  1:2942618/1‑93 (MQ=255)
                           cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcc                                                               >  1:4080094/1‑93 (MQ=255)
                           cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGCGGCGCTCGGGGCGTTTAcctcc                                                               >  2:2360508/1‑93 (MQ=255)
                             gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                             >  2:2215246/1‑93 (MQ=255)
                             gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                             >  2:1069465/1‑93 (MQ=255)
                             gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                             <  2:3027423/93‑1 (MQ=255)
                               ttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTcc                                                           >  2:1012786/1‑93 (MQ=255)
                                    ctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtg                                                      <  2:922647/93‑1 (MQ=255)
                                     tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                     >  1:2687428/1‑93 (MQ=255)
                                      gctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgat                                                    >  1:178339/1‑93 (MQ=255)
                                       ctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatg                                                   >  2:5642351/1‑93 (MQ=255)
                                       ctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatg                                                   >  2:3250399/1‑93 (MQ=255)
                                       ctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatg                                                   <  2:4971116/93‑1 (MQ=255)
                                        tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                  >  1:410861/1‑93 (MQ=255)
                                         gATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgat                                                 >  2:6065027/1‑93 (MQ=255)
                                         gATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGCTCGTTTACCTCCTTCCCGCtgatgat                                                 >  2:2710570/1‑93 (MQ=255)
                                          aTTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgatg                                                >  2:5337745/1‑93 (MQ=255)
                                          aTTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgatg                                                <  2:3274135/93‑1 (MQ=255)
                                           ttGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgatga                                               >  1:1114260/1‑93 (MQ=255)
                                             tctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGc                                             <  2:1749621/92‑1 (MQ=255)
                                             gctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGc                                             >  1:840436/1‑93 (MQ=255)
                                             gctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGc                                             >  1:1883761/1‑93 (MQ=255)
                                             gctgcgAACGGTGTGGGTATGGTTAGCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGGTTACCTCCTTCCCGTTGATGTTGTGc                                             >  1:5462552/1‑93 (MQ=255)
                                              ctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCt                                            >  1:1301249/1‑93 (MQ=255)
                                               tgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCtt                                           >  2:4587507/1‑93 (MQ=255)
                                                 ctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGc                                         >  1:6002511/1‑93 (MQ=255)
                                                 ctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGc                                         >  2:367900/1‑93 (MQ=255)
                                                  tAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCt                                        >  1:1484949/1‑93 (MQ=255)
                                                   aaCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTg                                       <  1:1662762/93‑1 (MQ=255)
                                                     cGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTggg                                     <  2:5841450/93‑1 (MQ=255)
                                                     cGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTggg                                     <  2:2274044/93‑1 (MQ=255)
                                                       gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                   <  1:5678966/93‑1 (MQ=255)
                                                       gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                   >  2:5069676/1‑93 (MQ=255)
                                                          tgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTgg                                >  1:868489/1‑93 (MQ=255)
                                                            ggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCg                              >  1:1789624/1‑93 (MQ=255)
                                                             gTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                             <  2:3369399/93‑1 (MQ=255)
                                                             gTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                             >  2:4860435/1‑93 (MQ=255)
                                                             gTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                             >  1:3818031/1‑93 (MQ=255)
                                                              tATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGt                            <  1:489215/93‑1 (MQ=255)
                                                              tATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGt                            >  2:5615288/1‑93 (MQ=255)
                                                              tATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGt                            >  2:1607426/1‑93 (MQ=255)
                                                                tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTct                          >  2:3342669/1‑92 (MQ=255)
                                                                 ggTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAt                         >  1:2082450/1‑93 (MQ=255)
                                                                 ggTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAt                         >  2:4513569/1‑93 (MQ=255)
                                                                 ggTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAt                         >  1:3660988/1‑93 (MQ=255)
                                                                 ggTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAt                         >  2:141204/1‑93 (MQ=255)
                                                                   ttATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCt                       >  2:6148541/1‑93 (MQ=255)
                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  2:1338429/93‑1 (MQ=255)
                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  1:76766/93‑1 (MQ=255)
                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  1:1700808/93‑1 (MQ=255)
                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  1:5583519/93‑1 (MQ=255)
                                                                    tATCAAAAACCCGATTGAAGGCTTGACAGTGGCGCTCGGTGCGTTTTCCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  2:2298698/93‑1 (MQ=255)
                                                                     aTCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTc                     >  2:3118402/1‑93 (MQ=255)
                                                                     aTCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTc                     <  1:4172078/93‑1 (MQ=255)
                                                                       cAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCgg                   <  1:6103174/93‑1 (MQ=255)
                                                                        aaaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGc                  <  1:363272/93‑1 (MQ=255)
                                                                         aaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGcc                 <  2:449006/93‑1 (MQ=255)
                                                                          aaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCt                <  2:2897728/93‑1 (MQ=255)
                                                                          aaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCt                >  2:2701829/1‑93 (MQ=255)
                                                                           aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg               >  2:5055246/1‑93 (MQ=255)
                                                                           aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg               >  1:513262/1‑93 (MQ=255)
                                                                            aCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTgg              >  2:3783773/1‑93 (MQ=255)
                                                                             cccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGga             <  2:5207595/93‑1 (MQ=255)
                                                                                gATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGaa          <  2:2391524/93‑1 (MQ=255)
                                                                                gATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGaa          >  1:1058101/1‑93 (MQ=255)
                                                                                 aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAg         >  1:3290389/1‑93 (MQ=255)
                                                                                 aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAg         >  1:5309264/1‑93 (MQ=255)
                                                                                  ttGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgt        <  1:1810031/93‑1 (MQ=255)
                                                                                   tGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtg       <  2:1802233/93‑1 (MQ=255)
                                                                                    gAAGGCTTGACAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtgt      <  2:5462964/93‑1 (MQ=255)
                                                                                     aaGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTc     <  2:4799280/93‑1 (MQ=255)
                                                                                     aaGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTc     <  1:3149150/93‑1 (MQ=255)
                                                                                      aGGCTTGCCAGTGGCGCTTGGTGCGTTTACCTCCTTCCCCGTGATGATGAGCTTGCTGGGGCTAGCGGTCATCTTCGGCCTGGAGGAGTGTcg    >  1:2779312/1‑93 (MQ=255)
                                                                                      aGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcg    >  1:5056972/1‑93 (MQ=255)
                                                                                       ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   <  2:3571599/93‑1 (MQ=255)
                                                                                       ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   <  1:66869/93‑1 (MQ=255)
                                                                                        gCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgcg  <  2:587221/93‑1 (MQ=255)
                                                                                             |                                                                                       
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG  >  NC_000913/3025583‑3025762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCG  >  NC_000913/3025583‑3025768
                                                                                              |                                                                                              
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                                >  SRR4302532.398311/1‑94 (MQ=60)
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                                >  SRR4302532.4143098/1‑94 (MQ=60)
 ctgccgcTACAGGGATCGGTATCGGGATGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAGACCCGATTGAAGGCTTG‑C‑C                                                                                              <  SRR4302532.5754040/87‑1 (MQ=60)
 CTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C‑C                                                                                              <  SRR4302532.1752173/94‑1 (MQ=60)
   CACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑Ccag                                                                                             >  SRR4302532.2396528/1‑91 (MQ=60)
   CACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑Ccag                                                                                             <  SRR4302532.4501902/94‑4 (MQ=60)
     CGGCACAGGTATCTGTATTGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTTGGGATGGTTATCTAAAACCCGATTGAATGCTTGCC‑AG‑TG                                                                                          >  SRR4302532.5678997/1‑94 (MQ=60)
      GGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGG                                                                                         <  SRR4302532.4652697/94‑1 (MQ=60)
      GGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGG                                                                                         <  SRR4302532.5514423/94‑1 (MQ=60)
       GTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGC                                                                                        <  SRR4302532.2974662/94‑1 (MQ=60)
        TACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCG                                                                                       >  SRR4302532.5944461/1‑94 (MQ=60)
          CAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCT                                                                                     <  SRR4302532.1868317/94‑1 (MQ=60)
           AGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGTGTATGGTTATCTAAAACTCGATTGAAGGCCTGCC‑AG‑TGGCGCTC                                                                                    >  SRR4302532.3334607/1‑94 (MQ=60)
            GGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCG                                                                                   >  SRR4302532.4257280/1‑94 (MQ=60)
              TATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGT                                                                                 <  SRR4302532.3221636/94‑1 (MQ=60)
                   GTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTT                                                                            >  SRR4302532.6071961/1‑94 (MQ=60)
                       CGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACC                                                                        <  SRR4302532.2100826/94‑1 (MQ=60)
                        GGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCT                                                                       <  SRR4302532.5949034/94‑1 (MQ=60)
                        GGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCT                                                                       >  SRR4302532.2640578/1‑94 (MQ=60)
                          GCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCC                                                                     >  SRR4302532.3040304/1‑94 (MQ=60)
                          GCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCC                                                                     >  SRR4302532.4217093/1‑94 (MQ=60)
                          GCTGGTTCTTCTGCTGATTGCTGCTGACGTTGTGGGTATGGTTATCAAATACCCGCTTGAATGCTTGCC‑AG‑TGGCGATCGTTTCGTTTACCTCC                                                                     <  SRR4302532.2277628/94‑1 (MQ=26)
                             GTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTC                                                                  >  SRR4302532.5701948/1‑94 (MQ=60)
                              TTTCTTCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTTCCTCCTTCC                                                                 <  SRR4302532.538249/94‑1 (MQ=60)
                              TTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCC                                                                 >  SRR4302532.2485547/1‑94 (MQ=60)
                               TTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCC                                                                <  SRR4302532.126351/94‑1 (MQ=60)
                                 CTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGG                                                              >  SRR4302532.498180/1‑94 (MQ=60)
                                    CTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGA                                                           >  SRR4302532.2777692/1‑94 (MQ=60)
                                     TGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGAT                                                          >  SRR4302532.699365/1‑94 (MQ=60)
                                     TGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGAT                                                          >  SRR4302532.184322/1‑94 (MQ=60)
                                       CTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGA                                                        >  SRR4302532.425182/1‑94 (MQ=60)
                                        TTATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGAT                                                       <  SRR4302532.640239/94‑1 (MQ=60)
                                         GATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATG                                                      >  SRR4302532.232586/1‑94 (MQ=60)
                                          ATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGA                                                     >  SRR4302532.1151498/1‑94 (MQ=60)
                                           TTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAG                                                    >  SRR4302532.5097544/1‑94 (MQ=60)
                                            TGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGC                                                   >  SRR4302532.869272/1‑94 (MQ=60)
                                            TGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGC                                                   >  SRR4302532.1946221/1‑94 (MQ=60)
                                            TGCTGCGAACGGTGTGGGTATGGTTAGCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGGTTACCTCCTTCCCGTTGATGTTGTGC                                                   >  SRR4302532.5651936/1‑94 (MQ=60)
                                             GCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCT                                                  >  SRR4302532.1344805/1‑94 (MQ=60)
                                              CTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTT                                                 >  SRR4302532.1945263/1‑94 (MQ=60)
                                               TGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTG                                                <  SRR4302532.4198006/94‑1 (MQ=60)
                                                GCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGC                                               >  SRR4302532.6209811/1‑94 (MQ=60)
                                                 CTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCT                                              >  SRR4302532.1535032/1‑94 (MQ=60)
                                                 CTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCT                                              >  SRR4302532.4294345/1‑94 (MQ=60)
                                                   AACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGG                                            <  SRR4302532.1719090/94‑1 (MQ=60)
                                                     CGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGG                                          <  SRR4302532.397979/94‑1 (MQ=60)
                                                     CGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGG                                          >  SRR4302532.5247671/1‑94 (MQ=60)
                                                     CGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGG                                          <  SRR4302532.1753188/94‑1 (MQ=60)
                                                       GTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCT                                        <  SRR4302532.5877234/94‑1 (MQ=60)
                                                         GTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGG                                      >  SRR4302532.898248/1‑94 (MQ=60)
                                                           GGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCG                                    >  SRR4302532.1849426/1‑94 (MQ=60)
                                                            GGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGG                                   >  SRR4302532.3946931/1‑94 (MQ=60)
                                                              TATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTC                                 <  SRR4302532.506310/94‑1 (MQ=60)
                                                               ATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCA                                >  SRR4302532.3854635/1‑94 (MQ=60)
                                                               ATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCA                                >  SRR4302532.3773492/1‑94 (MQ=60)
                                                                TGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAT                               >  SRR4302532.3783909/1‑94 (MQ=60)
                                                                TGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAT                               >  SRR4302532.2151876/1‑94 (MQ=60)
                                                                 GGTTATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGGTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATC                              <  SRR4302532.1743638/94‑1 (MQ=60)
                                                                    TATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTC                           <  SRR4302532.5777347/94‑1 (MQ=60)
                                                                    TATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTC                           <  SRR4302532.1758181/94‑1 (MQ=60)
                                                                    TATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTC                           <  SRR4302532.79506/94‑1 (MQ=60)
                                                                     ATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCG                          <  SRR4302532.4311995/94‑1 (MQ=60)
                                                                     ATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCG                          >  SRR4302532.6099793/1‑94 (MQ=60)
                                                                     ATCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCG                          <  SRR4302532.4039393/94‑1 (MQ=60)
                                                                      TCAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGG                         >  SRR4302532.1832178/1‑94 (MQ=60)
                                                                       CAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGC                        <  SRR4302532.4247758/94‑1 (MQ=60)
                                                                       CAAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGC                        <  SRR4302532.6313796/94‑1 (MQ=60)
                                                                        AAAGACCCGATTGAGGGCTTG‑C‑CGTTGGCGCTCGGTGCGTGCACCTCCTTCGCGGTGATGATGAGCTTGCTGGAGCAGGCGGTCATCTTCGGCC                       <  SRR4302532.375843/94‑1 (MQ=48)
                                                                        AAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCC                       <  SRR4302532.2878816/94‑1 (MQ=60)
                                                                        AAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCC                       >  SRR4302532.3701283/1‑94 (MQ=60)
                                                                        AAAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCC                       <  SRR4302532.375848/94‑1 (MQ=60)
                                                                         ttcgggatATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCT                      <  SRR4302532.2805583/86‑1 (MQ=60)
                                                                         AAAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCT                      >  SRR4302532.5933255/1‑94 (MQ=60)
                                                                          AAACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTG                     >  SRR4302532.531175/1‑94 (MQ=60)
                                                                           AACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGG                    >  SRR4302532.3268319/1‑94 (MQ=60)
                                                                           AACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTAATCTTCGGCCTGG                    >  SRR4302532.2773553/1‑94 (MQ=60)
                                                                            ACCCGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGA                   >  SRR4302532.6284713/1‑94 (MQ=60)
                                                                               CGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAA                >  SRR4302532.162625/1‑94 (MQ=60)
                                                                               CGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAA                >  SRR4302532.811116/1‑94 (MQ=60)
                                                                               CGATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAA                >  SRR4302532.1093761/1‑94 (MQ=60)
                                                                                GATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAG               >  SRR4302532.3399853/1‑94 (MQ=60)
                                                                                GATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAG               >  SRR4302532.5492853/1‑94 (MQ=60)
                                                                                 ATTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGT              >  SRR4302532.1844564/1‑94 (MQ=60)
                                                                                 ATGGAAGGCTTG‑CGAG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGT              <  SRR4302532.4223401/94‑1 (MQ=60)
                                                                                  TTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTG             <  SRR4302532.5482800/94‑1 (MQ=60)
                                                                                  TTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTG             <  SRR4302532.1524771/94‑1 (MQ=60)
                                                                                  TTGAAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTG             <  SRR4302532.1870400/94‑1 (MQ=60)
                                                                                     AAGGCTTGCC‑AG‑TGGCGCTTGGTGCGTTTACCTCCTTCCCCGTGATGATGAGCTTGCTGGGGCTAGCGGTCATCTTCGGCCTGGAGGAGTGTCG          >  SRR4302532.2872559/1‑94 (MQ=60)
                                                                                     AAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCG          <  SRR4302532.5797294/94‑1 (MQ=60)
                                                                                     AAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCG          >  SRR4302532.5230545/1‑94 (MQ=60)
                                                                                     AAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCG          <  SRR4302532.3253630/94‑1 (MQ=60)
                                                                                     AAGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCG          >  SRR4302532.934838/1‑94 (MQ=60)
                                                                                      AGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGC         <  SRR4302532.4393089/94‑1 (MQ=60)
                                                                                      AGGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGC         <  SRR4302532.9041/94‑1 (MQ=60)
                                                                                       GGCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG        <  SRR4302532.69273/94‑1 (MQ=60)
                                                                                        GCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGGGAAGCGTCGCGT       >  SRR4302532.3876844/1‑94 (MQ=60)
                                                                                        GCTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGT       >  SRR4302532.3876845/1‑94 (MQ=60)
                                                                                         CTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTA      >  SRR4302532.5920182/1‑94 (MQ=60)
                                                                                         CTTGCC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTA      >  SRR4302532.2345190/1‑94 (MQ=60)
                                                                                            gcC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC   <  SRR4302532.5794239/92‑1 (MQ=60)
                                                                                            gcC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC   <  SRR4302532.2416241/92‑1 (MQ=60)
                                                                                            gcC‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC   >  SRR4302532.3709469/3‑94 (MQ=60)
                                                                                            C‑C‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCG  <  SRR4302532.6151616/94‑1 (MQ=60)
                                                                                            C‑C‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTCGCGGTCATCTTTGGGCTGGGGTAGTGTCGTCTACCCG  >  SRR4302532.4354907/1‑94 (MQ=60)
                                                                                              |                                                                                              
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C‑AG‑TGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCG  >  NC_000913/3025583‑3025768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑