Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,025,676 +C coding (535/570 nt) ygfQ → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,025,6751.C100.0% 767.1 / NA 207L178F (TTG→TTC) ygfQhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (103/104);  total (103/104)

Only 100 of 234 total aligned reads displayed.

GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG  >  NC_000913/3025583‑3025762
                                                                                             |                                                                                       
gCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTg                                                                                          >  2:2196145/1‑93 (MQ=255)
gCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTg                                                                                          <  1:5814539/93‑1 (MQ=255)
 cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑c                                                                                        >  2:1520493/1‑93 (MQ=255)
 cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑c                                                                                        <  2:335004/93‑1 (MQ=255)
 cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑c                                                                                        >  1:3307165/1‑93 (MQ=255)
  tCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGGTTGAAGGCTTG‑cc                                                                                       >  1:1003094/1‑92 (MQ=255)
  tCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cc                                                                                       <  2:2310995/93‑2 (MQ=255)
   cACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGATTG‑cca                                                                                      >  2:1379775/1‑91 (MQ=255)
     cGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAATCCGATTGAAGGCTTG‑ccagt                                                                                    >  1:1031092/1‑89 (MQ=255)
       gTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                   <  1:988373/93‑1 (MQ=255)
       gTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                   <  2:558844/93‑1 (MQ=255)
          cAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGgcgc                                                                                <  2:639602/93‑1 (MQ=255)
           aGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCt                                                                               <  2:2394651/93‑1 (MQ=255)
           aGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCt                                                                               >  1:3417935/1‑93 (MQ=255)
           aGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCt                                                                               <  1:2424700/93‑1 (MQ=255)
              tatcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCgg                                                                            >  2:5551513/1‑93 (MQ=255)
               atcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGt                                                                           <  1:227370/93‑1 (MQ=255)
               atcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGt                                                                           >  1:145244/1‑93 (MQ=255)
               atcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGt                                                                           <  2:6372390/93‑1 (MQ=255)
               atcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGt                                                                           <  1:5843914/93‑1 (MQ=255)
                 cggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGc                                                                         <  2:3816857/93‑1 (MQ=255)
                   gtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGt                                                                       >  2:578163/1‑93 (MQ=255)
                   gtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGt                                                                       >  2:3455087/1‑93 (MQ=255)
                     atcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGttt                                                                     <  1:157303/93‑1 (MQ=255)
                      tcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTa                                                                    >  2:1608829/1‑93 (MQ=255)
                      tcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTa                                                                    >  2:4963363/1‑93 (MQ=255)
                      tcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGGGGCGCTCGGTGCGtttc                                                                    >  1:543844/1‑92 (MQ=255)
                       cggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAc                                                                   >  2:4729571/1‑93 (MQ=255)
                       cggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAc                                                                   >  1:395212/1‑93 (MQ=255)
                          gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctc                                                                <  2:3098336/93‑1 (MQ=255)
                           cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcc                                                               >  2:855290/1‑93 (MQ=255)
                            tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcct                                                              <  2:5670455/93‑1 (MQ=255)
                             gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                             >  2:3071571/1‑93 (MQ=255)
                             gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                             >  2:2713861/1‑93 (MQ=255)
                              tttCTGCTGCTGATTGCTGCTAACGGGGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCttcttc                                                            >  2:260533/1‑93 (MQ=255)
                               ttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTcc                                                           <  1:193038/93‑1 (MQ=255)
                               ttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTcc                                                           <  2:5052818/93‑1 (MQ=255)
                                  tgctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCgg                                                        <  2:3942671/93‑1 (MQ=255)
                                  tgctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCgg                                                        <  1:5337075/93‑1 (MQ=255)
                                   gctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGt                                                       <  1:475146/93‑1 (MQ=255)
                                   gctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGt                                                       <  1:2725625/93‑1 (MQ=255)
                                    ctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtg                                                      >  2:515339/1‑93 (MQ=255)
                                     tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                     >  2:3881621/1‑93 (MQ=255)
                                     tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                     >  2:1820433/1‑93 (MQ=255)
                                     tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                     <  1:2180303/93‑1 (MQ=255)
                                       ctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatg                                                   >  2:4583823/1‑93 (MQ=255)
                                       ctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatg                                                   >  1:5920097/1‑93 (MQ=255)
                                        tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                  <  2:1080012/93‑1 (MQ=255)
                                        tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                  >  1:6614736/1‑93 (MQ=255)
                                        tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                  <  2:2901021/93‑1 (MQ=255)
                                         gATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgat                                                 <  2:5955405/93‑1 (MQ=255)
                                         gATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgat                                                 <  1:6318651/93‑1 (MQ=255)
                                             gctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGc                                             <  2:4071538/93‑1 (MQ=255)
                                              ctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCt                                            >  1:3908277/1‑93 (MQ=255)
                                              ctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCt                                            <  2:6199205/93‑1 (MQ=255)
                                               tgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCtt                                           <  1:1499625/93‑1 (MQ=255)
                                               tgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCtt                                           <  2:1301716/93‑1 (MQ=255)
                                                  tAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCt                                        <  2:3148208/93‑1 (MQ=255)
                                                  tAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCt                                        <  2:4160599/93‑1 (MQ=255)
                                                   aaCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTg                                       >  2:3106967/1‑93 (MQ=255)
                                                   aaCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTg                                       <  1:4524359/93‑1 (MQ=255)
                                                    aCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTgg                                      >  2:2082654/1‑93 (MQ=255)
                                                        tgtgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCCGTGATGATGAGCTTGCTGGGGCt                                  >  1:1265959/1‑93 (MQ=255)
                                                          tgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTgg                                <  1:1721787/93‑1 (MQ=255)
                                                          tgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTgg                                <  2:483305/93‑1 (MQ=255)
                                                          tgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTgg                                >  1:3854724/1‑93 (MQ=255)
                                                          tgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTgg                                >  2:3612054/1‑93 (MQ=255)
                                                           gggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGc                               <  1:5986079/93‑1 (MQ=255)
                                                           gggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGc                               <  1:6078606/93‑1 (MQ=255)
                                                             gTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                             <  2:945774/93‑1 (MQ=255)
                                                              tATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGt                            >  1:3687141/1‑93 (MQ=255)
                                                               aTGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTc                           >  1:5223710/1‑93 (MQ=255)
                                                                tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCa                          >  1:885620/1‑93 (MQ=255)
                                                                 ggTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAt                         <  1:2477264/93‑1 (MQ=255)
                                                                  gTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATc                        <  2:2025365/93‑1 (MQ=255)
                                                                   ttATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCt                       <  1:772535/93‑1 (MQ=255)
                                                                    tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      <  2:5006816/93‑1 (MQ=255)
                                                                      tCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCg                    <  1:5639084/93‑1 (MQ=255)
                                                                      tCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCg                    <  1:4318877/93‑1 (MQ=255)
                                                                      tCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCg                    <  2:5425328/93‑1 (MQ=255)
                                                                         aaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGcc                 <  2:3534847/93‑1 (MQ=255)
                                                                         aaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGcc                 <  1:4928525/93‑1 (MQ=255)
                                                                          aaaCCCGATTGAAGTCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCt                <  2:3763908/93‑1 (MQ=255)
                                                                           aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg               >  2:4880106/1‑93 (MQ=255)
                                                                           aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg               >  1:3546311/1‑93 (MQ=255)
                                                                             cccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCTGCCTggg             >  1:4159924/1‑92 (MQ=255)
                                                                             cccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGga             >  1:4854831/1‑93 (MQ=255)
                                                                               cGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGgaga           >  2:1873332/1‑93 (MQ=255)
                                                                               cGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGgaga           <  2:412622/93‑1 (MQ=255)
                                                                                 aTTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAg         >  2:4261525/1‑93 (MQ=255)
                                                                                  ttGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgt        <  1:3142187/93‑1 (MQ=255)
                                                                                   tGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtg       >  2:1880383/1‑93 (MQ=255)
                                                                                   tGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtg       <  1:1165128/93‑1 (MQ=255)
                                                                                    gAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtgt      >  1:2817918/1‑93 (MQ=255)
                                                                                    gAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtgt      <  1:6414242/93‑1 (MQ=255)
                                                                                     aaGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTc     <  2:4478727/93‑1 (MQ=255)
                                                                                       ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   <  2:5282994/93‑1 (MQ=255)
                                                                                       ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   <  2:242597/93‑1 (MQ=255)
                                                                                       agCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   <  2:5912864/92‑1 (MQ=255)
                                                                                        gCTTGCCAGTGGCGCTCTGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgcg  <  1:3571755/93‑1 (MQ=255)
                                                                                             |                                                                                       
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG  >  NC_000913/3025583‑3025762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGG  >  NC_000913/3025583‑3025769
                                                                                              |                                                                                             
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                               >  SRR4302547.1712873/1‑94 (MQ=60)
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                               >  SRR4302547.113980/1‑94 (MQ=60)
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                               >  SRR4302547.8209/1‑94 (MQ=60)
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                               >  SRR4302547.3373835/1‑94 (MQ=60)
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                               <  SRR4302547.5934843/94‑1 (MQ=60)
 CTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGGTTGAAGGCTTG‑CC                                                                                              >  SRR4302547.1022664/1‑94 (MQ=60)
 CTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CC                                                                                              >  SRR4302547.4291989/1‑94 (MQ=60)
  TCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑Cca                                                                                             >  SRR4302547.4155526/1‑92 (MQ=60)
    ACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAATCCGATTGAAGGCTTGCCAGT                                                                                            >  SRR4302547.1051358/1‑94 (MQ=60)
    ACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGT                                                                                            >  SRR4302547.1603639/1‑94 (MQ=60)
      GGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGG                                                                                          >  SRR4302547.3426565/1‑94 (MQ=60)
      GGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGG                                                                                          <  SRR4302547.4645780/94‑1 (MQ=60)
      CGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGG                                                                                          <  SRR4302547.2039751/94‑1 (MQ=60)
       GTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC                                                                                         <  SRR4302547.1007612/94‑1 (MQ=60)
         ACAGGTATCGGTATCGGGGTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGC                                                                                       >  SRR4302547.3967528/1‑94 (MQ=60)
         ACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGGGTGGGTTTGGTTATCAAAAACCCGATTAAAGGCTTG‑Ccaggggggc                                                                                      >  SRR4302547.1203958/1‑85 (MQ=60)
          CAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCT                                                                                      >  SRR4302547.3487531/1‑94 (MQ=60)
          CAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCT                                                                                      <  SRR4302547.498597/94‑1 (MQ=60)
          CAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCCGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCT                                                                                      <  SRR4302547.541792/94‑1 (MQ=60)
           AGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTC                                                                                     <  SRR4302547.2473766/94‑1 (MQ=60)
             GTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGG                                                                                   <  SRR4302547.5307463/94‑1 (MQ=60)
             GTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGG                                                                                   >  SRR4302547.5682635/1‑94 (MQ=60)
              TATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGT                                                                                  >  SRR4302547.147863/1‑94 (MQ=60)
               ATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTG                                                                                 <  SRR4302547.5964806/94‑1 (MQ=60)
               ATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTG                                                                                 <  SRR4302547.231639/94‑1 (MQ=60)
                TCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGC                                                                                >  SRR4302547.6343429/1‑94 (MQ=60)
                 CGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCG                                                                               <  SRR4302547.690045/94‑1 (MQ=60)
                 CGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCG                                                                               >  SRR4302547.2878982/1‑94 (MQ=60)
                  GGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGT                                                                              >  SRR4302547.4798333/1‑94 (MQ=60)
                   GTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTT                                                                             >  SRR4302547.3653415/1‑94 (MQ=60)
                   GTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTT                                                                             <  SRR4302547.2948455/94‑1 (MQ=60)
                    TATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTT                                                                            >  SRR4302547.6265889/1‑94 (MQ=60)
                     ATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTA                                                                           >  SRR4302547.3641641/1‑94 (MQ=60)
                     ATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTA                                                                           <  SRR4302547.160186/94‑1 (MQ=60)
                     ATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGGGGCGCTCGGTGCGTTTC                                                                           >  SRR4302547.553663/1‑94 (MQ=60)
                      TCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAC                                                                          >  SRR4302547.402554/1‑94 (MQ=60)
                              TTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCGGTGGCGCTCGGTGCGTTTACCTCCTTCC                                                                  >  SRR4302547.5557944/1‑94 (MQ=60)
                              TTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCC                                                                  <  SRR4302547.3287856/94‑1 (MQ=60)
                               TTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCC                                                                 <  SRR4302547.4000936/94‑1 (MQ=60)
                               TTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCC                                                                 <  SRR4302547.196656/94‑1 (MQ=60)
                                  TGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGT                                                              <  SRR4302547.5447091/94‑1 (MQ=60)
                                   GCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTG                                                             <  SRR4302547.2780351/94‑1 (MQ=60)
                                   GCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTG                                                             <  SRR4302547.483871/94‑1 (MQ=60)
                                    CTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGA                                                            >  SRR4302547.3889432/1‑94 (MQ=60)
                                    CTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGA                                                            >  SRR4302547.984430/1‑94 (MQ=60)
                                     TGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGAT                                                           <  SRR4302547.2223972/94‑1 (MQ=60)
                                     CGCTGATTGCTGCTACCGGTGTGGGTATGGTTATCAAAATCCAGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGAT                                                           <  SRR4302547.4851192/94‑1 (MQ=60)
                                      GCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATG                                                          >  SRR4302547.6042793/1‑94 (MQ=60)
                                      GCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATG                                                          <  SRR4302547.119263/94‑1 (MQ=60)
                                       CTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGA                                                         >  SRR4302547.6752419/1‑94 (MQ=60)
                                        TGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGAT                                                        >  SRR4302547.2482465/1‑94 (MQ=60)
                                        TGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGAT                                                        <  SRR4302547.1260052/94‑1 (MQ=60)
                                        TGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGAT                                                        >  SRR4302547.6639561/1‑94 (MQ=60)
                                         GATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATG                                                       <  SRR4302547.6450038/94‑1 (MQ=60)
                                          ATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGA                                                      <  SRR4302547.4210820/94‑1 (MQ=60)
                                             GCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCT                                                   >  SRR4302547.2735272/1‑94 (MQ=60)
                                             GCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCT                                                   <  SRR4302547.1504440/94‑1 (MQ=60)
                                             GCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCT                                                   >  SRR4302547.3987756/1‑94 (MQ=60)
                                             GCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTCAAGGCTTGCCAGTTGCGCCCGGTGCGTTTACCTACTTACCCGTGATGATGATCT                                                   >  SRR4302547.1324203/1‑94 (MQ=60)
                                               TGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTG                                                 <  SRR4302547.1529537/94‑1 (MQ=60)
                                               TGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTG                                                 >  SRR4302547.35156/1‑94 (MQ=60)
                                                   AACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGG                                             <  SRR4302547.4617854/94‑1 (MQ=60)
                                                      GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGC                                          >  SRR4302547.1571250/1‑94 (MQ=60)
                                                       GTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCT                                         <  SRR4302547.6096439/94‑1 (MQ=60)
                                                       GTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCCGTGATGATGAGCTTGCTGGGGCT                                         >  SRR4302547.1291459/1‑94 (MQ=60)
                                                         GTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGG                                       >  SRR4302547.3933188/1‑94 (MQ=60)
                                                          TGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC                                      <  SRR4302547.1755635/94‑1 (MQ=60)
                                                          TGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC                                      <  SRR4302547.2299909/94‑1 (MQ=60)
                                                           GGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCG                                     <  SRR4302547.6204699/94‑1 (MQ=60)
                                                           GGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCG                                     <  SRR4302547.6110257/94‑1 (MQ=60)
                                                           GGGTATGGTTATCAAAAACACGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGACGATGAGCTTGCTGGGGTTGGCG                                     >  SRR4302547.2062250/1‑94 (MQ=60)
                                                             GTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGT                                   <  SRR4302547.6144213/94‑1 (MQ=60)
                                                             GTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGT                                   >  SRR4302547.3762415/1‑94 (MQ=60)
                                                              TATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTC                                  >  SRR4302547.5948715/1‑94 (MQ=60)
                                                              TATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTC                                  >  SRR4302547.5331232/1‑94 (MQ=60)
                                                               ATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCA                                 >  SRR4302547.902637/1‑94 (MQ=60)
                                                                TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAT                                >  SRR4302547.2519485/1‑94 (MQ=60)
                                                                 GGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATC                               <  SRR4302547.2527344/94‑1 (MQ=60)
                                                                  GTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCT                              >  SRR4302547.6526385/1‑94 (MQ=60)
                                                                  GTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCT                              >  SRR4302547.2852202/1‑94 (MQ=60)
                                                                   TTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTT                             <  SRR4302547.787136/94‑1 (MQ=60)
                                                                    TATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTC                            <  SRR4302547.5322217/94‑1 (MQ=60)
                                                                     ATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCG                           >  SRR4302547.1336850/1‑94 (MQ=60)
                                                                      TCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGG                          <  SRR4302547.4407772/94‑1 (MQ=60)
                                                                      TCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGG                          <  SRR4302547.578760/94‑1 (MQ=60)
                                                                      TCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGG                          <  SRR4302547.5755484/94‑1 (MQ=60)
                                                                       CAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGC                         <  SRR4302547.3610188/94‑1 (MQ=60)
                                                                        AAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTACCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCCGCC                        >  SRR4302547.2111517/1‑94 (MQ=60)
                                                                         AAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCT                       <  SRR4302547.5030613/94‑1 (MQ=60)
                                                                          AAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTG                      >  SRR4302547.3618683/1‑94 (MQ=60)
                                                                           AACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGAGGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGG                     >  SRR4302547.1038959/1‑94 (MQ=60)
                                                                            ACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCTGCCTGGG                    >  SRR4302547.4244678/1‑94 (MQ=60)
                                                                            ACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGA                    >  SRR4302547.4955414/1‑94 (MQ=60)
                                                                               CGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAA                 >  SRR4302547.5986772/1‑94 (MQ=60)
                                                                                GATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAG                <  SRR4302547.4549332/94‑1 (MQ=60)
                                                                                 ATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGT               >  SRR4302547.3922179/1‑94 (MQ=60)
                                                                                 ATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGT               >  SRR4302547.1945071/1‑94 (MQ=60)
                                                                                  TTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTG              <  SRR4302547.3204609/94‑1 (MQ=60)
                                                                                   TGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGT             >  SRR4302547.2874287/1‑94 (MQ=60)
                                                                                   TGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGT             <  SRR4302547.1188698/94‑1 (MQ=60)
                                                                                   TGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGT             >  SRR4302547.5532649/1‑94 (MQ=60)
                                                                                    GAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTC            <  SRR4302547.6547999/94‑1 (MQ=60)
                                                                                     AAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCG           >  SRR4302547.781034/1‑94 (MQ=60)
                                                                                      AGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGC          <  SRR4302547.2356556/94‑1 (MQ=60)
                                                                                      AGGCTTGCCAGTGGCGCTCGGTGCGTTTAACTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGGAGTGTCGC          >  SRR4302547.6003100/1‑94 (MQ=60)
                                                                                       GGCTTGCCAGTGGCGCTCGGTGCGTTTGCCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG         <  SRR4302547.4655052/94‑1 (MQ=60)
                                                                                        GCTTGCCAGTGGCGCTCTGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGT        <  SRR4302547.3644617/94‑1 (MQ=60)
                                                                                         CTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTA       <  SRR4302547.1882625/94‑1 (MQ=60)
                                                                                          TTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTAC      >  SRR4302547.4409752/1‑94 (MQ=60)
                                                                                          TTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTAC      <  SRR4302547.803041/94‑1 (MQ=60)
                                                                                           tgcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTGCC     >  SRR4302547.6742542/4‑94 (MQ=60)
                                                                                           tgcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACC     >  SRR4302547.1376578/4‑94 (MQ=60)
                                                                                            gcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC    <  SRR4302547.3998458/92‑1 (MQ=60)
                                                                                            gcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC    >  SRR4302547.2532517/3‑94 (MQ=60)
                                                                                            C‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCG   <  SRR4302547.3884225/94‑1 (MQ=60)
                                                                                              CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGG  <  SRR4302547.2808054/94‑1 (MQ=60)
                                                                                              |                                                                                             
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGG  >  NC_000913/3025583‑3025769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑