Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,029,284 T→C intergenic (‑318/‑34) ygfT ← / → ygfU putative oxidoreductase Fe‑S subunit/putative permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,029,2840TC83.7% 617.6 / 106.1 251intergenic (‑318/‑34)ygfT/ygfUputative oxidoreductase Fe‑S subunit/putative permease
Reads supporting (aligned to +/- strand):  ref base T (21/20);  new base C (112/98);  total (133/118)
Fisher's exact test for biased strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 260 total aligned reads displayed.

AAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAA  >  NC_000913/3029192‑3029373
                                                                                            |                                                                                         
 aaaTTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTc                                                                                          >  1:4006772/1‑93 (MQ=255)
   aTTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACcccc                                                                                        >  1:1192756/1‑93 (MQ=255)
    ttACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAAcccccc                                                                                       >  1:1377788/1‑93 (MQ=255)
     tACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCt                                                                                      <  2:5863772/93‑1 (MQ=255)
      aCAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCtt                                                                                     <  2:5986904/93‑1 (MQ=255)
        aGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTcc                                                                                   >  1:758624/1‑93 (MQ=255)
        aGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTcc                                                                                   <  2:4418522/93‑1 (MQ=255)
        aGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTcc                                                                                   >  1:4349857/1‑93 (MQ=255)
        aGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTcc                                                                                   >  1:3305802/1‑93 (MQ=255)
        aGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTcc                                                                                   <  1:1556795/93‑1 (MQ=255)
         gATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCt                                                                                  >  1:2047742/1‑93 (MQ=255)
         gATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCt                                                                                  >  1:3033551/1‑93 (MQ=255)
           tAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTcc                                                                                >  2:4427819/1‑92 (MQ=255)
             aTTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCa                                                                              >  2:1568670/1‑93 (MQ=255)
                ccATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCaaaa                                                                           >  1:2223118/1‑93 (MQ=255)
                 cATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCaaaaa                                                                          >  1:6175713/1‑93 (MQ=255)
                  aTTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAAc                                                                         >  2:272592/1‑93 (MQ=255)
                   ttGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACt                                                                        <  2:6178593/93‑1 (MQ=255)
                   ttGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACt                                                                        >  1:4688698/1‑93 (MQ=255)
                    tGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTg                                                                       <  1:993015/93‑1 (MQ=255)
                      tCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGc                                                                     <  1:2690342/93‑1 (MQ=255)
                       cAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCa                                                                    <  2:4802064/93‑1 (MQ=255)
                        aGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCAc                                                                   <  1:664390/93‑1 (MQ=255)
                        aGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCAc                                                                   <  1:320047/93‑1 (MQ=255)
                          gTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTc                                                                 <  1:1780298/93‑1 (MQ=255)
                           ttctcttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTcc                                                                <  2:1886478/93‑1 (MQ=255)
                           ttctcttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTcc                                                                >  2:5048463/1‑93 (MQ=255)
                             ctcttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCAc                                                              >  2:3050619/1‑93 (MQ=255)
                             ctcttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCAc                                                              >  1:472182/1‑93 (MQ=255)
                              tcttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACg                                                             >  1:3373902/1‑93 (MQ=255)
                              tcttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACg                                                             >  2:1582209/1‑93 (MQ=255)
                              tcttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCACCCTTCCTCGCAAACACTGGCACTCCACg                                                             >  2:6326698/1‑93 (MQ=255)
                               cttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTACCTCCCCTTCCTCGCAAAAACTGGCACTCCACGa                                                            <  1:128052/93‑1 (MQ=255)
                               cttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGa                                                            <  1:6508324/93‑1 (MQ=255)
                               cttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGa                                                            <  1:3334275/93‑1 (MQ=255)
                                ttctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAg                                                           >  2:3859073/1‑93 (MQ=255)
                                  ctcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCa                                                         <  2:3096448/93‑1 (MQ=255)
                                   tcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAt                                                        >  1:2703706/1‑93 (MQ=255)
                                   tcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAt                                                        >  2:5723742/1‑93 (MQ=255)
                                   tcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAt                                                        <  2:5536070/93‑1 (MQ=255)
                                   tcATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAt                                                        >  1:4418630/1‑93 (MQ=255)
                                    cATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAtg                                                       <  1:384769/93‑1 (MQ=255)
                                    cATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAtg                                                       <  2:1010644/93‑1 (MQ=255)
                                     aTTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAtgt                                                      <  1:1980745/93‑1 (MQ=255)
                                      ttttCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAtgtg                                                     <  2:2979982/93‑1 (MQ=255)
                                       tttCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAACCTGGCACTCCACGAGCATgtgt                                                    <  1:3322651/93‑1 (MQ=255)
                                       tttCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATgtgt                                                    <  2:3651801/93‑1 (MQ=255)
                                          cGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTa                                                 <  1:2863318/93‑1 (MQ=255)
                                              gTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACa                                             >  2:2199884/1‑93 (MQ=255)
                                               tttAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAg                                            <  2:865160/93‑1 (MQ=255)
                                               tttAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAg                                            <  2:757300/93‑1 (MQ=255)
                                                ttAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGt                                           <  1:820307/93‑1 (MQ=255)
                                                ttAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGt                                           <  2:4355205/93‑1 (MQ=255)
                                                ttAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGt                                           <  2:4108845/93‑1 (MQ=255)
                                                 tAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGtt                                          <  1:1271471/93‑1 (MQ=255)
                                                 tAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGtt                                          <  1:3720553/93‑1 (MQ=255)
                                                 tAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGtt                                          >  2:2811132/1‑93 (MQ=255)
                                                  aaCCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACTAGCATGTGTTTAGACAGttt                                         <  1:2156026/93‑1 (MQ=255)
                                                  aaCCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGttt                                         >  1:5784152/1‑93 (MQ=255)
                                                    ccACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCa                                       >  2:1082846/1‑93 (MQ=255)
                                                    ccACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCa                                       >  2:396061/1‑93 (MQ=255)
                                                     cACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCAt                                      <  2:1397988/93‑1 (MQ=255)
                                                     cACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCAt                                      >  1:6110577/1‑93 (MQ=255)
                                                      aCGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCAtt                                     >  2:1895207/1‑93 (MQ=255)
                                                      aCGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCAtt                                     <  2:4677785/93‑1 (MQ=255)
                                                       cGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTa                                    <  1:2435588/93‑1 (MQ=255)
                                                        gATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTaa                                   <  2:1255585/93‑1 (MQ=255)
                                                         aTTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAAc                                  >  2:4223680/1‑93 (MQ=255)
                                                             tgtgATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTaa                              >  2:6521996/1‑93 (MQ=255)
                                                              gtgATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTaaa                             <  1:2349006/93‑1 (MQ=255)
                                                              gtgATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTaaa                             <  1:5837050/93‑1 (MQ=255)
                                                               tgATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAAc                            <  1:2286245/93‑1 (MQ=255)
                                                               tgATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAAc                            >  2:5394553/1‑93 (MQ=255)
                                                                gATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACg                           <  1:1940905/93‑1 (MQ=255)
                                                                 aTTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACgg                          >  1:541416/1‑93 (MQ=255)
                                                                 aTTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACgg                          >  2:4627506/1‑93 (MQ=255)
                                                                  ttGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGt                         >  2:3017548/1‑93 (MQ=255)
                                                                  ttGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGt                         <  1:4131818/93‑1 (MQ=255)
                                                                   tGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGtt                        >  1:4442699/1‑93 (MQ=255)
                                                                      cTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCt                     >  2:5271413/1‑93 (MQ=255)
                                                                      cTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCCCTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCt                     <  1:539204/93‑1 (MQ=255)
                                                                      cTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCt                     >  1:345844/1‑93 (MQ=255)
                                                                       tCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCtt                    >  1:2527681/1‑93 (MQ=255)
                                                                       tCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCtt                    >  2:4994980/1‑93 (MQ=255)
                                                                        ccGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCttt                   <  1:5494476/93‑1 (MQ=255)
                                                                         cGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCtttt                  <  1:1604134/93‑1 (MQ=255)
                                                                          gCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCttttt                 <  2:404302/93‑1 (MQ=255)
                                                                          gCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCttttt                 >  2:456789/1‑93 (MQ=255)
                                                                           cAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTa                <  1:3240350/93‑1 (MQ=255)
                                                                           cAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTa                >  1:3654710/1‑93 (MQ=255)
                                                                           cAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTa                >  2:2038040/1‑93 (MQ=255)
                                                                            aaTTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAc               >  1:6293862/1‑93 (MQ=255)
                                                                            aaTTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAc               >  2:2642546/1‑93 (MQ=255)
                                                                             aTTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAct              >  2:3433180/1‑93 (MQ=255)
                                                                                ccTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTg           <  1:494001/93‑1 (MQ=255)
                                                                                     ctctAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTggcggg      <  2:487186/93‑1 (MQ=255)
                                                                                       ctAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTggcgggcg    >  1:1043662/1‑93 (MQ=255)
                                                                                        tAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGa   <  2:1733594/93‑1 (MQ=255)
                                                                                        tAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGa   >  1:2984368/1‑93 (MQ=255)
                                                                                        tAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGa   <  2:3577182/93‑1 (MQ=255)
                                                                                            |                                                                                         
AAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAA  >  NC_000913/3029192‑3029373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAAGGA  >  NC_000913/3029191‑3029377
                                                                                             |                                                                                             
GAAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACT                                                                                               >  SRR4302547.3681915/1‑94 (MQ=60)
 AAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTC                                                                                              >  SRR4302547.4088212/1‑94 (MQ=60)
 AAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTAAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCC                                                                                              <  SRR4302547.3730270/94‑1 (MQ=60)
   AATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCC                                                                                            <  SRR4302547.1016362/94‑1 (MQ=60)
   AATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCC                                                                                            >  SRR4302547.1216825/1‑94 (MQ=60)
    ATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCC                                                                                           >  SRR4302547.2003652/1‑94 (MQ=60)
    ATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCC                                                                                           >  SRR4302547.1405410/1‑94 (MQ=60)
    ATTACAGATAATTCCAATGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCC                                                                                           >  SRR4302547.340/1‑94 (MQ=60)
     TTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCT                                                                                          <  SRR4302547.2775635/94‑1 (MQ=60)
      TACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTT                                                                                         >  SRR4302547.5533026/1‑94 (MQ=60)
      TACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTT                                                                                         <  SRR4302547.5509146/94‑1 (MQ=60)
       ACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTC                                                                                        <  SRR4302547.3379534/94‑1 (MQ=60)
        CAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCC                                                                                       <  SRR4302547.2926028/94‑1 (MQ=60)
        CAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCC                                                                                       <  SRR4302547.802928/94‑1 (MQ=60)
        CAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCC                                                                                       >  SRR4302547.772924/1‑94 (MQ=60)
        CAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCC                                                                                       >  SRR4302547.3372422/1‑94 (MQ=60)
        CAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCC                                                                                       >  SRR4302547.6397817/1‑94 (MQ=60)
        CAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCC                                                                                       >  SRR4302547.3317941/1‑94 (MQ=60)
        CAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCC                                                                                       >  SRR4302547.4439240/1‑94 (MQ=60)
         AGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCT                                                                                      >  SRR4302547.2088584/1‑94 (MQ=60)
         AGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCT                                                                                      >  SRR4302547.3093880/1‑94 (MQ=60)
         AGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCT                                                                                      >  SRR4302547.4133356/1‑94 (MQ=60)
         AGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCT                                                                                      <  SRR4302547.1587768/94‑1 (MQ=60)
            TAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGC                                                                                   >  SRR4302547.2570328/1‑94 (MQ=60)
             AATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCA                                                                                  >  SRR4302547.1800499/1‑94 (MQ=60)
                TCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAA                                                                               >  SRR4302547.2267905/1‑94 (MQ=60)
                TCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAA                                                                               >  SRR4302547.3226795/1‑94 (MQ=60)
                 CCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAA                                                                              >  SRR4302547.6303901/1‑94 (MQ=60)
                 CCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAA                                                                              >  SRR4302547.1273462/1‑94 (MQ=60)
                   ATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACT                                                                            >  SRR4302547.4038508/1‑94 (MQ=60)
                   ATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACT                                                                            >  SRR4302547.667362/1‑94 (MQ=60)
                   ATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACT                                                                            >  SRR4302547.4785805/1‑94 (MQ=60)
                     TGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGG                                                                          <  SRR4302547.1012359/94‑1 (MQ=60)
                       TCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCA                                                                        <  SRR4302547.2744349/94‑1 (MQ=60)
                         AGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACT                                                                      <  SRR4302547.2286192/94‑1 (MQ=60)
                         AGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACT                                                                      <  SRR4302547.676621/94‑1 (MQ=60)
                         AGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACT                                                                      <  SRR4302547.325998/94‑1 (MQ=60)
                         AGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGAACT                                                                      >  SRR4302547.5620816/1‑94 (MQ=60)
                           GTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCC                                                                    >  SRR4302547.313809/1‑94 (MQ=60)
                           GTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCC                                                                    <  SRR4302547.1815581/94‑1 (MQ=60)
                           GGTCTCTTCTCATTTTCGTGGGTTAACCACGATTATGTGATGGCCTCCGCGATTGCTGTCTCTAACCCCCCTTACTCTCATTAACTGGCACTCC                                                                    >  SRR4302547.5753841/1‑94 (MQ=48)
                            TTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCA                                                                   >  SRR4302547.1303948/1‑94 (MQ=60)
                            TTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCA                                                                   >  SRR4302547.6105390/1‑94 (MQ=60)
                            TTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCA                                                                   >  SRR4302547.1948488/1‑94 (MQ=60)
                             TCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCAC                                                                  >  SRR4302547.480861/1‑94 (MQ=60)
                              CTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACG                                                                 >  SRR4302547.3442441/1‑94 (MQ=60)
                              CTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACG                                                                 >  SRR4302547.3963076/1‑94 (MQ=60)
                               TCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGA                                                                >  SRR4302547.3769330/1‑94 (MQ=60)
                                CTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTACCTCCCCTTCCTCGCAAAAACTGGCACTCCACGAG                                                               <  SRR4302547.130368/94‑1 (MQ=60)
                                CTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAG                                                               >  SRR4302547.6679890/1‑94 (MQ=60)
                                CTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAG                                                               <  SRR4302547.6643834/94‑1 (MQ=60)
                                CTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAG                                                               <  SRR4302547.3401708/94‑1 (MQ=60)
                                 TTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGC                                                              >  SRR4302547.4791924/1‑94 (MQ=60)
                                 TTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGC                                                              <  SRR4302547.2247374/94‑1 (MQ=60)
                                  TCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCA                                                             >  SRR4302547.1999683/1‑94 (MQ=60)
                                  TCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCA                                                             >  SRR4302547.2070949/1‑94 (MQ=60)
                                   CTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAT                                                            >  SRR4302547.2757965/1‑94 (MQ=60)
                                   CTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAT                                                            >  SRR4302547.72750/1‑94 (MQ=60)
                                   CTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAT                                                            <  SRR4302547.5933972/94‑1 (MQ=60)
                                   CTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAT                                                            >  SRR4302547.4509827/1‑94 (MQ=60)
                                    TCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATG                                                           >  SRR4302547.2377382/1‑94 (MQ=60)
                                     CATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGT                                                          <  SRR4302547.391901/94‑1 (MQ=60)
                                      ATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTG                                                         <  SRR4302547.2020076/94‑1 (MQ=60)
                                        TTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAACCTGGCACTCCACGAGCATGTGTT                                                       <  SRR4302547.3389774/94‑1 (MQ=60)
                                          TCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTA                                                     <  SRR4302547.121047/94‑1 (MQ=60)
                                          TCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTA                                                     <  SRR4302547.1635468/94‑1 (MQ=60)
                                           CGTGGTTTAGCCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAG                                                    <  SRR4302547.1543543/94‑1 (MQ=60)
                                           CGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAG                                                    <  SRR4302547.2920517/94‑1 (MQ=60)
                                            GTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCAGGTGTTTAGA                                                   <  SRR4302547.6591540/94‑1 (MQ=60)
                                             TGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGaattgcggaggcaatcacataatc                                                  <  SRR4302547.4680120/94‑25 (MQ=60)
                                             TGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGAC                                                  >  SRR4302547.4344698/1‑94 (MQ=60)
                                               GTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAG                                                <  SRR4302547.4468596/94‑1 (MQ=60)
                                                TTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGT                                               >  SRR4302547.1673856/1‑94 (MQ=60)
                                                 TTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTT                                              <  SRR4302547.835943/94‑1 (MQ=60)
                                                  TAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTT                                             <  SRR4302547.3351381/94‑1 (MQ=60)
                                                  TAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTT                                             <  SRR4302547.3382833/94‑1 (MQ=60)
                                                  TAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTT                                             >  SRR4302547.5903794/1‑94 (MQ=60)
                                                  TAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTT                                             <  SRR4302547.1297076/94‑1 (MQ=60)
                                                  TAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTT                                             <  SRR4302547.3796500/94‑1 (MQ=60)
                                                   AACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTC                                            >  SRR4302547.264580/1‑94 (MQ=60)
                                                   AACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACTAGCATGTGTTTAGACAGTTTC                                            <  SRR4302547.2199092/94‑1 (MQ=60)
                                                    ACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCA                                           >  SRR4302547.286143/1‑94 (MQ=60)
                                                    ACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGATTCA                                           >  SRR4302547.1575474/1‑94 (MQ=60)
                                                     CCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCAT                                          >  SRR4302547.6237329/1‑94 (MQ=60)
                                                     CCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCAT                                          <  SRR4302547.3072040/94‑1 (MQ=60)
                                                     CCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCCCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCAT                                          >  SRR4302547.3492598/1‑94 (MQ=60)
                                                      CACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATT                                         <  SRR4302547.3268478/94‑1 (MQ=60)
                                                        CGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAA                                       <  SRR4302547.2484852/94‑1 (MQ=60)
                                                         GATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAAC                                      <  SRR4302547.2595437/94‑1 (MQ=60)
                                                             ATGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAA                                  <  SRR4302547.298112/94‑1 (MQ=60)
                                                              TGTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAA                                 >  SRR4302547.341637/1‑94 (MQ=60)
                                                               GTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAAC                                <  SRR4302547.2396615/94‑1 (MQ=60)
                                                               GTGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAAC                                <  SRR4302547.5957826/94‑1 (MQ=60)
                                                                TGATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACG                               <  SRR4302547.2332532/94‑1 (MQ=60)
                                                                 TATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGG                              <  SRR4302547.2182283/94‑1 (MQ=60)
                                                                 GATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGG                              >  SRR4302547.206019/1‑94 (MQ=60)
                                                                 GATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGG                              >  SRR4302547.551188/1‑94 (MQ=60)
                                                                 GATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGG                              <  SRR4302547.1979398/94‑1 (MQ=60)
                                                                  ATTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGT                             <  SRR4302547.4405481/94‑1 (MQ=60)
                                                                   TTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTT                            >  SRR4302547.4534584/1‑94 (MQ=60)
                                                                   TTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTT                            <  SRR4302547.4215886/94‑1 (MQ=60)
                                                                   TTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTT                            <  SRR4302547.1330854/94‑1 (MQ=60)
                                                                   TTGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTT                            <  SRR4302547.1240424/94‑1 (MQ=60)
                                                                    TGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTG                           >  SRR4302547.6103013/1‑94 (MQ=60)
                                                                    TGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTG                           <  SRR4302547.3758640/94‑1 (MQ=60)
                                                                    TGCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTG                           <  SRR4302547.3904293/94‑1 (MQ=60)
                                                                     GCCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGC                          <  SRR4302547.4466464/94‑1 (MQ=60)
                                                                      CCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCT                         >  SRR4302547.4706422/1‑94 (MQ=60)
                                                                      CCTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCT                         >  SRR4302547.352273/1‑94 (MQ=60)
                                                                       CTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTT                        >  SRR4302547.740235/1‑94 (MQ=60)
                                                                       CTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCCCTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTT                        <  SRR4302547.548940/94‑1 (MQ=60)
                                                                       CTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTT                        >  SRR4302547.2461061/1‑94 (MQ=60)
                                                                       CTCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTT                        >  SRR4302547.2578768/1‑94 (MQ=60)
                                                                        TCCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTT                       <  SRR4302547.6351221/94‑1 (MQ=60)
                                                                         CCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTT                      >  SRR4302547.2358040/1‑94 (MQ=60)
                                                                         CCGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTT                      <  SRR4302547.5607921/94‑1 (MQ=60)
                                                                          CGCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTT                     <  SRR4302547.1635953/94‑1 (MQ=60)
                                                                           GCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTA                    >  SRR4302547.5956817/1‑94 (MQ=60)
                                                                           GCAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTA                    >  SRR4302547.3729266/1‑94 (MQ=60)
                                                                            CAATTCCTGTCTCTATCCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAC                   <  SRR4302547.3563049/94‑1 (MQ=60)
                                                                            CAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAC                   <  SRR4302547.5119329/94‑1 (MQ=60)
                                                                            CAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAC                   >  SRR4302547.5030896/1‑94 (MQ=60)
                                                                            CAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAC                   <  SRR4302547.3305030/94‑1 (MQ=60)
                                                                            CAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAC                   >  SRR4302547.1378835/1‑94 (MQ=60)
                                                                            CAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAC                   >  SRR4302547.6424699/1‑94 (MQ=60)
                                                                            CAATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTAC                   >  SRR4302547.867736/1‑94 (MQ=60)
                                                                             AATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACT                  >  SRR4302547.4959711/1‑94 (MQ=60)
                                                                              ATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTC                 >  SRR4302547.3405402/1‑94 (MQ=60)
                                                                              ATTCCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTC                 >  SRR4302547.4532046/1‑94 (MQ=60)
                                                                                TCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTG               <  SRR4302547.6273006/94‑1 (MQ=60)
                                                                                 CCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGG              <  SRR4302547.5227895/94‑1 (MQ=60)
                                                                                 CCTGTCTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGG              <  SRR4302547.503016/94‑1 (MQ=60)
                                                                                      CTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGC         <  SRR4302547.2226472/94‑1 (MQ=60)
                                                                                      CTCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGC         >  SRR4302547.2483697/1‑94 (MQ=60)
                                                                                       TCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCG        <  SRR4302547.911210/94‑1 (MQ=60)
                                                                                       TCTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCG        >  SRR4302547.1064260/1‑94 (MQ=60)
                                                                                        CTAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGA       >  SRR4302547.3043760/1‑94 (MQ=60)
                                                                                         TAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAA      >  SRR4302547.4765076/1‑94 (MQ=60)
                                                                                         TAACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAA      >  SRR4302547.554118/1‑94 (MQ=60)
                                                                                          AACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTGGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAA     <  SRR4302547.2745684/94‑1 (MQ=60)
                                                                                          AACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAA     >  SRR4302547.287272/1‑94 (MQ=60)
                                                                                          AACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAA     >  SRR4302547.5229946/1‑94 (MQ=60)
                                                                                          AACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAA     >  SRR4302547.1551668/1‑94 (MQ=60)
                                                                                          AACCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAA     >  SRR4302547.6065149/1‑94 (MQ=60)
                                                                                            CCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGGAAGG   >  SRR4302547.6040402/1‑94 (MQ=60)
                                                                                            CCCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAAGG   <  SRR4302547.1221946/94‑1 (MQ=60)
                                                                                             CCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAAGGA  >  SRR4302547.6078982/1‑94 (MQ=60)
                                                                                             CCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAAGGA  >  SRR4302547.1571041/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAAAATTACAGATAATTCCATTGTCAGGTTCTCTTCTCATTTTCGTGGTTTAACCACGATTATGTGATTGCCTCCGCAATTCCTGTCTCTAACTCCCCTTCCTCGCAAAAACTGGCACTCCACGAGCATGTGTTTAGACAGTTTCATTAACGTAAACGGTTGCTTTTTACTCTGGCGGGCGAAAGGA  >  NC_000913/3029191‑3029377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑