Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,099,661 (A)5→6 intergenic (‑19/+164) yggN ← / ← b2959 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,099,6561.A98.8% 939.2 / 2.5 249intergenic (‑14/+169)yggN/b2959hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base A (111/135);  total (114/135)
Fisher's exact test for biased strand distribution p-value = 9.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

Only 100 of 274 total aligned reads displayed.

TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT  >  NC_000913/3099564‑3099745
                                                                                             |                                                                                          
 gACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑a                                                                                           <  2:5641751/93‑1 (MQ=255)
   cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa                                                                                         <  2:4081313/93‑1 (MQ=255)
   cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa                                                                                         <  1:1658710/93‑1 (MQ=255)
    gCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa‑a                                                                                       <  2:1708483/93‑1 (MQ=255)
      tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa‑aaa                                                                                     >  1:3705566/1‑92 (MQ=255)
       gCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa‑aaat                                                                                    >  2:2607533/1‑91 (MQ=255)
         aCTGGTAGGCGGCGTGAGCGGTCATGGCCGTCACTGAAAGTGCTGCCGCCAGCAGGATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa‑aaatca                                                                                  <  1:1754070/93‑5 (MQ=255)
           tGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAgg                                                                                 <  1:5552502/93‑1 (MQ=255)
           tGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAgg                                                                                 >  2:2140992/1‑93 (MQ=255)
           tGGTAGTCGGCGTGAGCGGTCATTGCCGGCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAgg                                                                                 <  2:1839275/93‑1 (MQ=255)
             gTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGcc                                                                               <  2:5589484/93‑1 (MQ=255)
              tAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCa                                                                              <  2:3327401/93‑1 (MQ=255)
              tAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCa                                                                              >  1:2671237/1‑93 (MQ=255)
                gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGc                                                                            <  1:6142940/93‑1 (MQ=255)
                gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGc                                                                            <  1:5228442/93‑1 (MQ=255)
                 tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCa                                                                           <  1:2128720/93‑1 (MQ=255)
                 tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCa                                                                           <  1:1285513/93‑1 (MQ=255)
                 tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCa                                                                           >  2:2718144/1‑93 (MQ=255)
                   ggCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCAtt                                                                         <  1:1698257/93‑1 (MQ=255)
                   ggCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCAtt                                                                         <  1:4616832/93‑1 (MQ=255)
                     cGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTg                                                                       <  2:2459986/93‑1 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGc                                                                      <  1:2793589/93‑1 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGc                                                                      >  1:5379296/1‑93 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCt                                                                     >  2:4606573/1‑93 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCt                                                                     <  1:4136110/93‑1 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCt                                                                     >  2:6116039/1‑93 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCt                                                                     <  2:5233758/93‑1 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTg                                                                    <  2:3634754/93‑1 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTg                                                                    <  1:4408059/93‑1 (MQ=255)
                           cGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGcc                                                                 <  2:1134260/93‑1 (MQ=255)
                           cGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAGAATCAGGCCAGCATTTGCTGGcc                                                                 >  1:3486109/1‑93 (MQ=255)
                             gTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGcccc                                                               >  2:2380154/1‑93 (MQ=255)
                             gTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGcccc                                                               >  1:3382676/1‑93 (MQ=255)
                             gTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGcccc                                                               <  2:3704669/93‑1 (MQ=255)
                              tCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCa                                                              <  1:986008/93‑1 (MQ=255)
                              tCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCa                                                              >  1:2891928/1‑93 (MQ=255)
                                aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGa                                                            >  2:3239937/1‑93 (MQ=255)
                                aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGa                                                            >  2:3942283/1‑93 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGAt                                                           >  2:3422999/1‑93 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGAt                                                           <  1:3203011/93‑1 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGAt                                                           >  2:3574225/1‑93 (MQ=255)
                                  tGCCGTCACTTAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGAtt                                                          <  2:2773768/93‑1 (MQ=255)
                                      gTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATa                                                      <  1:2511716/93‑1 (MQ=255)
                                       tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                     >  1:1198023/1‑93 (MQ=255)
                                       tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                     >  2:5828154/1‑93 (MQ=255)
                                       tCACTGAAAGTGCTGCCCCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                     <  2:2790274/93‑1 (MQ=255)
                                            gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg                                                <  1:5313797/93‑1 (MQ=255)
                                            gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg                                                >  2:5531598/1‑93 (MQ=255)
                                             aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt                                               <  1:4198610/93‑1 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                              <  2:4374757/93‑1 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                              <  2:2420812/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc                                             <  2:3076145/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc                                             <  1:477221/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc                                             <  1:3057847/93‑1 (MQ=255)
                                                gTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcg                                            <  2:603236/93‑1 (MQ=255)
                                                  gctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTAAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg                                          <  2:4456333/93‑1 (MQ=255)
                                                   ctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGt                                         >  2:148593/1‑93 (MQ=255)
                                                     gccgccAGCAGCATTTTGCGCATCATAGTCTTCGCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                       >  1:567016/1‑93 (MQ=255)
                                                     gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                       >  2:1963540/1‑93 (MQ=255)
                                                       cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                     <  1:721115/93‑1 (MQ=255)
                                                        gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                    >  1:145197/1‑93 (MQ=255)
                                                        gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                    <  2:4290450/93‑1 (MQ=255)
                                                         ccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                                   >  2:5016167/1‑93 (MQ=255)
                                                         ccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                                   <  1:4705860/93‑1 (MQ=255)
                                                           agcagcATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATg                                 >  1:3004756/1‑93 (MQ=255)
                                                           agcagcATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATg                                 >  1:1109539/1‑93 (MQ=255)
                                                              agcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                              >  2:826224/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                             >  2:5722843/1‑93 (MQ=255)
                                                                caTTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                            >  2:2535285/1‑93 (MQ=255)
                                                                caTTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                            <  1:4884571/93‑1 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                           >  2:903819/1‑93 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                          >  2:4500677/1‑93 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                          >  2:1546906/1‑93 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                          >  2:4753488/1‑93 (MQ=255)
                                                                       cgcATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcg                     >  1:227002/1‑93 (MQ=255)
                                                                        gcATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcgc                    >  1:5027301/1‑92 (MQ=255)
                                                                        gcATCATAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcgc                    >  1:1519828/1‑92 (MQ=255)
                                                                         catcatAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcgca                   >  2:3187421/1‑91 (MQ=255)
                                                                         catcatAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTa                   <  2:92917/93‑1 (MQ=255)
                                                                          atcatAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                  >  1:3310038/1‑93 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTaaa                 >  2:2253047/1‑93 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaaa                 <  2:5368994/93‑1 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCAAtctgg                              >  2:1218752/1‑76 (MQ=255)
                                                                            catAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAac                >  1:3865508/1‑93 (MQ=255)
                                                                            catAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAac                <  2:4186517/93‑1 (MQ=255)
                                                                             atAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTaaaac               >  1:1907267/1‑91 (MQ=255)
                                                                             atAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAaca               >  1:2856163/1‑93 (MQ=255)
                                                                             atAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAaca               >  1:4509366/1‑93 (MQ=255)
                                                                              tAGTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAacac              >  1:2728444/1‑93 (MQ=255)
                                                                                gTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCt            >  1:214860/1‑93 (MQ=255)
                                                                                gTCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCt            >  2:5132281/1‑93 (MQ=255)
                                                                                 tCTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCtt           <  1:3587699/93‑1 (MQ=255)
                                                                                  cTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTa          >  2:5771561/1‑93 (MQ=255)
                                                                                  cTTCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTa          <  1:1017048/93‑1 (MQ=38)
                                                                                   ttCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAt         <  1:5844033/93‑1 (MQ=255)
                                                                                    tCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATc        >  1:2088979/1‑93 (MQ=255)
                                                                                    tCCCTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATc        >  2:2072259/1‑93 (MQ=255)
                                                                                     cccTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATcc       >  1:3138277/1‑93 (MQ=255)
                                                                                     cccTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATcc       >  1:5149078/1‑93 (MQ=255)
                                                                                     cccTCAAGAAAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATcc       >  2:735679/1‑93 (MQ=255)
                                                                                             |                                                                                          
TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAA‑AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT  >  NC_000913/3099564‑3099745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  NC_000913/3099569‑3099754
                                                                                               |                                                                                             
CTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAA‑A‑AA                                                                                              >  SRR4302532.5995527/1‑94 (MQ=60)
CTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAA‑A‑AA                                                                                              >  SRR4302532.3829960/1‑94 (MQ=60)
  GCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAA‑A‑Aatc                                                                                            <  SRR4302532.2769646/94‑4 (MQ=60)
   CACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCA                                                                                            <  SRR4302532.3455338/94‑1 (MQ=60)
    ACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAG                                                                                           <  SRR4302532.5817869/94‑1 (MQ=60)
    ACTGGTAGGCGGCGTGAGCGGTCATGGCCGTCACTGAAAGTGCTGCCGCCAGCAGGATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAG                                                                                           <  SRR4302532.1812975/94‑1 (MQ=60)
      TGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGC                                                                                         >  SRR4302532.5311704/1‑94 (MQ=60)
      TGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGC                                                                                         <  SRR4302532.5745250/94‑1 (MQ=60)
       GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCC                                                                                        >  SRR4302532.1935075/1‑94 (MQ=60)
       GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCC                                                                                        <  SRR4302532.3282661/94‑1 (MQ=60)
        GTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCA                                                                                       >  SRR4302532.2760887/1‑94 (MQ=60)
         TAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAG                                                                                      >  SRR4302532.3228911/1‑94 (MQ=60)
         TAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAG                                                                                      <  SRR4302532.3583484/94‑1 (MQ=60)
           GTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCA                                                                                    <  SRR4302532.5408759/94‑1 (MQ=60)
           GTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCA                                                                                    <  SRR4302532.2296283/94‑1 (MQ=60)
           GTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCA                                                                                    <  SRR4302532.6354828/94‑1 (MQ=60)
           GTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCA                                                                                    >  SRR4302532.1104543/1‑94 (MQ=60)
            TCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCAT                                                                                   <  SRR4302532.1328510/94‑1 (MQ=60)
            TCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCAT                                                                                   <  SRR4302532.2199833/94‑1 (MQ=60)
             CGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATT                                                                                  >  SRR4302532.4773951/1‑94 (MQ=60)
              GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTT                                                                                 <  SRR4302532.487119/94‑1 (MQ=60)
              GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTT                                                                                 <  SRR4302532.1755561/94‑1 (MQ=60)
              GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTT                                                                                 <  SRR4302532.4774254/94‑1 (MQ=60)
               GTGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTG                                                                                <  SRR4302532.6058670/94‑1 (MQ=60)
               GCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTG                                                                                <  SRR4302532.556074/94‑1 (MQ=60)
                CGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGC                                                                               >  SRR4302532.5565631/1‑94 (MQ=60)
                 GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCT                                                                              <  SRR4302532.2887246/94‑1 (MQ=60)
                 GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCT                                                                              >  SRR4302532.2955103/1‑94 (MQ=60)
                  TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTG                                                                             <  SRR4302532.4684927/94‑1 (MQ=60)
                  TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTG                                                                             <  SRR4302532.4274854/94‑1 (MQ=60)
                  TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTG                                                                             <  SRR4302532.174447/94‑1 (MQ=60)
                   GAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGG                                                                            <  SRR4302532.3090363/94‑1 (MQ=60)
                   GAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGG                                                                            <  SRR4302532.4558205/94‑1 (MQ=60)
                   GAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGG                                                                            >  SRR4302532.3306940/1‑94 (MQ=60)
                     GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAGA‑ATCAGGCCAGCATTTGCTGGCC                                                                          >  SRR4302532.3602903/1‑94 (MQ=60)
                      CGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCC                                                                         <  SRR4302532.4080390/94‑1 (MQ=60)
                       GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCC                                                                        >  SRR4302532.3495811/1‑94 (MQ=60)
                        GTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCA                                                                       >  SRR4302532.2988529/1‑94 (MQ=60)
                        GTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCA                                                                       >  SRR4302532.220215/1‑94 (MQ=60)
                         TCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAG                                                                      <  SRR4302532.1019693/94‑1 (MQ=60)
                          CATTGCCGTCACTGAAATTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGA                                                                     <  SRR4302532.2585149/94‑1 (MQ=60)
                           ATTGCGGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    <  SRR4302532.537807/94‑1 (MQ=60)
                            TTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATT                                                                   <  SRR4302532.3309360/94‑1 (MQ=60)
                             TGCCGTCACTGAAAGAGCTGCCGCCAGCAGCATTTTGCGCATCATAGACTTCCCTCAAGAAAA‑A‑ATCAGGCCCGCATTTGCTGGCCCCAGAata                                                                  >  SRR4302532.2700118/1‑91 (MQ=60)
                               CCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGAT                                                                >  SRR4302532.5140076/1‑94 (MQ=60)
                               CCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGAT                                                                >  SRR4302532.3828053/1‑94 (MQ=60)
                                CGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATA                                                               <  SRR4302532.4581112/94‑1 (MQ=60)
                                CGTCACTGAAAGTGCTGCCGCCAACAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATA                                                               >  SRR4302532.4128849/1‑94 (MQ=60)
                                 GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                              <  SRR4302532.2595521/94‑1 (MQ=60)
                                 GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                              >  SRR4302532.1238083/1‑94 (MQ=60)
                                     CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAA                                                          >  SRR4302532.4962058/1‑94 (MQ=60)
                                      TGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAG                                                         <  SRR4302532.3802284/94‑1 (MQ=60)
                                       GAAAGTGCTGCCGCGAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302532.4781317/94‑1 (MQ=60)
                                       GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302532.5497590/94‑1 (MQ=60)
                                        AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                       <  SRR4302532.4339522/94‑1 (MQ=60)
                                        AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                       <  SRR4302532.3908378/94‑1 (MQ=60)
                                        AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                       <  SRR4302532.1227659/94‑1 (MQ=60)
                                        AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                       >  SRR4302532.2182716/1‑94 (MQ=60)
                                        AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                       <  SRR4302532.231356/94‑1 (MQ=60)
                                         AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                      >  SRR4302532.2982571/1‑94 (MQ=60)
                                         AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                      <  SRR4302532.318273/94‑1 (MQ=60)
                                         AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                      <  SRR4302532.1516139/94‑1 (MQ=60)
                                          AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     <  SRR4302532.3158814/94‑1 (MQ=60)
                                          AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     <  SRR4302532.493859/94‑1 (MQ=60)
                                          AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     <  SRR4302532.2903355/94‑1 (MQ=60)
                                               TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCGCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                >  SRR4302532.586813/1‑94 (MQ=60)
                                               TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                <  SRR4302532.3916546/94‑1 (MQ=60)
                                                GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                               <  SRR4302532.2159748/94‑1 (MQ=60)
                                                 CCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTC                                              <  SRR4302532.6328960/94‑1 (MQ=60)
                                                  CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                             >  SRR4302532.884587/1‑94 (MQ=60)
                                                  CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                             >  SRR4302532.150077/1‑94 (MQ=60)
                                                  CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                             <  SRR4302532.745727/94‑1 (MQ=60)
                                                   GCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCA                                            >  SRR4302532.1360301/1‑94 (MQ=60)
                                                    CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302532.4866412/94‑1 (MQ=60)
                                                     CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          <  SRR4302532.4558413/94‑1 (MQ=60)
                                                     CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          >  SRR4302532.1146636/1‑94 (MQ=60)
                                                     CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          >  SRR4302532.3103978/1‑94 (MQ=60)
                                                       GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                        >  SRR4302532.1163065/1‑94 (MQ=60)
                                                        CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       <  SRR4302532.1754453/94‑1 (MQ=60)
                                                         gatcttcgggatATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCaatctggg                                      <  SRR4302532.1259537/82‑9 (MQ=60)
                                                           CATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCGGCATTTCCTGCCCCCAGATGGATAACAAAGTACGCGTTTTCCATGCCGTAT                                    <  SRR4302532.4606500/94‑1 (MQ=60)
                                                           CATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    <  SRR4302532.5052491/94‑1 (MQ=60)
                                                           CATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    <  SRR4302532.4410912/94‑1 (MQ=60)
                                                           CATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302532.2148585/1‑94 (MQ=60)
                                                           CATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    <  SRR4302532.1764696/94‑1 (MQ=60)
                                                            ATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   <  SRR4302532.2149880/94‑1 (MQ=60)
                                                            ATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   >  SRR4302532.1323950/1‑94 (MQ=60)
                                                            ATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   <  SRR4302532.4284780/94‑1 (MQ=60)
                                                            ATTTTGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   <  SRR4302532.3109558/94‑1 (MQ=60)
                                                                TGCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGC                               <  SRR4302532.3591286/94‑1 (MQ=60)
                                                                 GCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                              >  SRR4302532.5491227/1‑94 (MQ=60)
                                                                 GCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                              >  SRR4302532.234754/1‑94 (MQ=60)
                                                                 GCGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                              <  SRR4302532.1286234/94‑1 (MQ=60)
                                                                  CGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGC                             >  SRR4302532.5199736/1‑94 (MQ=60)
                                                                  CGCATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGC                             >  SRR4302532.1571152/1‑94 (MQ=60)
                                                                    CATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGTCAGCATTTGCTGGCCCCAGATTGATACCAAAGTACGCGTTTTCCATGCGGGATGCGGCGCAA                           >  SRR4302532.5767959/1‑94 (MQ=60)
                                                                    CATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAA                           >  SRR4302532.4449205/1‑94 (MQ=60)
                                                                    CATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAA                           >  SRR4302532.1538357/1‑94 (MQ=60)
                                                                    CATCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAA                           >  SRR4302532.3420368/1‑94 (MQ=60)
                                                                      TCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAAC                         <  SRR4302532.1880382/94‑1 (MQ=60)
                                                                      TCATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                         >  SRR4302532.3995968/1‑94 (MQ=60)
                                                                      TCATAGTCTCCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                         <  SRR4302532.4890160/94‑1 (MQ=60)
                                                                       CATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAAac                        >  SRR4302532.1970557/1‑92 (MQ=60)
                                                                       CATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACA                        >  SRR4302532.2951668/1‑94 (MQ=60)
                                                                       CATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                        >  SRR4302532.4663330/1‑94 (MQ=60)
                                                                        ATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAC                       >  SRR4302532.2820184/1‑94 (MQ=60)
                                                                        ATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                       >  SRR4302532.5067706/1‑94 (MQ=60)
                                                                        ATAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                       >  SRR4302532.2670154/1‑94 (MQ=60)
                                                                         TAGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACC                      <  SRR4302532.696548/94‑1 (MQ=60)
                                                                          AGTCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT                     >  SRR4302532.222184/1‑94 (MQ=60)
                                                                            TCTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTA                   <  SRR4302532.3707714/94‑1 (MQ=60)
                                                                             CTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                  >  SRR4302532.5040710/1‑94 (MQ=60)
                                                                             CTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                  <  SRR4302532.590903/94‑1 (MQ=60)
                                                                             CTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302532.5514344/94‑1 (MQ=60)
                                                                             CTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  >  SRR4302532.3454099/1‑94 (MQ=60)
                                                                             CTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302532.1051574/94‑1 (MQ=60)
                                                                             CTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302532.3626942/94‑1 (MQ=60)
                                                                             CTTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302532.3137/94‑1 (MQ=60)
                                                                              TTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATC                 >  SRR4302532.2465727/1‑94 (MQ=60)
                                                                              TTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATC                 >  SRR4302532.2158656/1‑94 (MQ=60)
                                                                              TTCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATC                 <  SRR4302532.6046846/94‑1 (MQ=60)
                                                                               TCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCC                >  SRR4302532.5326210/1‑94 (MQ=60)
                                                                               TCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCC                <  SRR4302532.810716/94‑1 (MQ=60)
                                                                               TCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCC                >  SRR4302532.3242428/1‑94 (MQ=60)
                                                                               TCCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGATTTCCATGCCGGATGCGGCGTAAACACCTTATCC                >  SRR4302532.5326211/1‑94 (MQ=60)
                                                                                CCCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCC               <  SRR4302532.5704476/94‑1 (MQ=60)
                                                                                 CCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCCTGCCGGATGCGGCGTAAACACCTTATCCCC              >  SRR4302532.1670498/1‑94 (MQ=60)
                                                                                 CCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCG              <  SRR4302532.1676596/94‑1 (MQ=60)
                                                                                 CCTCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCG              >  SRR4302532.3379568/1‑94 (MQ=60)
                                                                                   TCAAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCC            >  SRR4302532.6086055/1‑94 (MQ=60)
                                                                                    CAAG‑AAA‑ACATCAGGCCAGCATTTGCTGGCCCCAGCTTGATAACAAAGTACGCGTTTTCCATGCAGGATGAGGCGCAAACACCTTATCCCGCCT           >  SRR4302532.2853669/1‑94 (MQ=60)
                                                                                     AAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA          >  SRR4302532.3083564/1‑94 (MQ=60)
                                                                                     AAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA          >  SRR4302532.5588517/1‑94 (MQ=60)
                                                                                     AAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA          >  SRR4302532.1865590/1‑94 (MQ=60)
                                                                                     AAGAAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTA          >  SRR4302532.576670/1‑94 (MQ=60)
                                                                                      agaAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTAC         >  SRR4302532.3345278/4‑94 (MQ=60)
                                                                                       gaAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACTCCTTATCCCGCCTACT        <  SRR4302532.3047522/92‑1 (MQ=60)
                                                                                       gaAAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACT        <  SRR4302532.5391975/92‑1 (MQ=60)
                                                                                       A‑AAA‑A‑ATCGGGCCAGCATTTGCTGGCCCGAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTC       <  SRR4302532.3968410/94‑1 (MQ=60)
                                                                                       A‑AAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTC       <  SRR4302532.2829664/94‑1 (MQ=60)
                                                                                         AAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCG      <  SRR4302532.16641/94‑1 (MQ=60)
                                                                                               ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  <  SRR4302532.705532/94‑1 (MQ=60)
                                                                                               |                                                                                             
CTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAA‑A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  NC_000913/3099569‑3099754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑