Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,099,661 (A)5→6 intergenic (‑19/+164) yggN ← / ← b2959 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,099,6561.A98.8% 953.7 / 2.8 244intergenic (‑14/+169)yggN/b2959hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base A (112/128);  total (115/129)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 259 total aligned reads displayed.

TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT  >  NC_000913/3099564‑3099745
                                                                                             |                                                                                          
      tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaa                                                                                      >  2:1970870/1‑92 (MQ=255)
      tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaa                                                                                      <  2:6207101/93‑2 (MQ=255)
       gCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCTCTCAAG‑aaaaaat                                                                                     >  2:4284771/1‑91 (MQ=255)
       gCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaat                                                                                     >  1:1067614/1‑91 (MQ=255)
         aCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatca                                                                                   <  2:294886/93‑5 (MQ=255)
             gTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGcc                                                                               >  2:3845979/1‑93 (MQ=255)
             gTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGcc                                                                               <  2:582384/93‑1 (MQ=255)
             gTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGcc                                                                               <  2:686236/93‑1 (MQ=255)
                gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGc                                                                            >  2:1716366/1‑93 (MQ=255)
                gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGc                                                                            >  1:140401/1‑93 (MQ=255)
                 tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCa                                                                           <  1:5796841/93‑1 (MQ=255)
                  cGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCAt                                                                          >  1:4358898/1‑93 (MQ=255)
                   ggCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCAtt                                                                         >  2:6181050/1‑93 (MQ=255)
                    gCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCAttt                                                                        <  2:5612208/93‑1 (MQ=255)
                     cGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTg                                                                       <  1:1650442/93‑1 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGc                                                                      <  1:4220752/93‑1 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCt                                                                     <  2:2287844/93‑1 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCt                                                                     <  1:446826/93‑1 (MQ=255)
                       ggAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCt                                                                     <  1:1011583/92‑1 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTg                                                                    >  1:3232787/1‑93 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTg                                                                    <  1:338939/93‑1 (MQ=255)
                         aGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTgg                                                                   <  1:2127564/93‑1 (MQ=255)
                         aGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTgg                                                                   >  2:1151122/1‑93 (MQ=255)
                          gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGc                                                                  <  1:6641302/93‑1 (MQ=255)
                          gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGc                                                                  >  2:5190197/1‑93 (MQ=255)
                            ggTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGccc                                                                >  1:2948570/1‑93 (MQ=255)
                            ggTCATGGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGccc                                                                <  1:1786695/93‑1 (MQ=255)
                               cATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAg                                                             >  2:4974744/1‑93 (MQ=255)
                                atgcccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGa                                                            <  2:580184/89‑1 (MQ=255)
                                aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGa                                                            <  2:4265043/93‑1 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGAt                                                           <  1:867527/93‑1 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGAt                                                           >  2:1875794/1‑93 (MQ=255)
                                   gCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTg                                                         >  2:2559236/1‑93 (MQ=255)
                                     cGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGAt                                                       >  2:5491740/1‑93 (MQ=255)
                                     cGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTATTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGAt                                                       >  1:4720471/1‑93 (MQ=255)
                                         acggAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACa                                                   <  2:1635988/90‑1 (MQ=255)
                                           tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa                                                 <  2:401620/93‑1 (MQ=255)
                                           tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa                                                 >  2:4684844/1‑93 (MQ=255)
                                            gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg                                                <  1:18473/93‑1 (MQ=255)
                                            gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg                                                >  2:3657546/1‑93 (MQ=255)
                                             aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt                                               >  1:6343990/1‑93 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                              >  2:4582663/1‑93 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                              <  1:5713410/93‑1 (MQ=255)
                                                gTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcg                                            <  2:2143849/93‑1 (MQ=255)
                                                 tgctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc                                           >  2:5737658/1‑93 (MQ=255)
                                                 tgctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc                                           <  2:5326215/93‑1 (MQ=255)
                                                 tgctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc                                           <  1:543113/93‑1 (MQ=255)
                                                   ctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGt                                         <  1:2527567/93‑1 (MQ=255)
                                                     gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                       >  2:5938619/1‑93 (MQ=255)
                                                      ccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                      >  2:4304769/1‑93 (MQ=255)
                                                      ccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                      >  1:147635/1‑93 (MQ=255)
                                                       cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                     <  2:504129/93‑1 (MQ=255)
                                                       cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                     >  2:1845601/1‑93 (MQ=255)
                                                       cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                     >  2:6100214/1‑93 (MQ=255)
                                                        gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                    <  1:5654184/93‑1 (MQ=255)
                                                        gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                    <  2:3478141/93‑1 (MQ=255)
                                                        gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                    >  1:2361196/1‑93 (MQ=255)
                                                          cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                                  <  2:3309880/93‑1 (MQ=255)
                                                          cagcagCATTTTGCGCATCATAGTCTCCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                                  <  1:4585728/93‑1 (MQ=255)
                                                           agcagcATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATg                                 <  1:2267246/93‑1 (MQ=255)
                                                            gcagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                                <  2:946140/93‑1 (MQ=255)
                                                            gcagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                                >  1:5173627/1‑93 (MQ=255)
                                                            gcagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCACTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                                >  2:6089790/1‑93 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                               <  2:2325119/93‑1 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                               >  2:1309420/1‑93 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                               >  1:1348750/1‑93 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                               <  2:6362639/93‑1 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                               <  1:6373894/93‑1 (MQ=255)
                                                              agcaTTTTGCGCATCATAGTGTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                              <  1:4678408/93‑1 (MQ=255)
                                                              agcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                              <  2:1134561/93‑1 (MQ=255)
                                                              agcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                              <  1:1582574/93‑1 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                             <  1:4000291/93‑1 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAAGCCgg                             >  2:2712931/1‑93 (MQ=255)
                                                                caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                            <  1:2576804/93‑1 (MQ=255)
                                                                caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                            >  1:4952634/1‑93 (MQ=255)
                                                                caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                            >  2:1469322/1‑93 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                           >  1:6075280/1‑93 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                           <  2:6653906/93‑1 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                           >  1:4045652/1‑93 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                           >  2:2257622/1‑93 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                          >  1:4306463/1‑93 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                          <  1:2748294/93‑1 (MQ=255)
                                                                    ttGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg                        <  1:5084133/93‑1 (MQ=255)
                                                                      gcgcATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggc                      <  1:2005767/93‑1 (MQ=255)
                                                                        gcATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAGAGTACGCGTTTTCCATGCCGGATGCGGCGt                    >  1:550138/1‑93 (MQ=255)
                                                                         catcatAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTa                   <  2:6289533/93‑1 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTaac                 >  1:5128974/1‑92 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTaaa                 >  1:3325674/1‑93 (MQ=255)
                                                                              tAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAacac              >  1:1883909/1‑93 (MQ=255)
                                                                               aGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAcc             <  1:5327697/93‑1 (MQ=255)
                                                                               aGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAcc             >  1:4889072/1‑93 (MQ=255)
                                                                               aGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAcc             <  1:306796/93‑1 (MQ=255)
                                                                                 tCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCtt           <  1:6524650/93‑1 (MQ=255)
                                                                                     cccTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATcc       >  1:5945733/1‑93 (MQ=255)
                                                                                      ccTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATccc      >  1:954200/1‑93 (MQ=255)
                                                                                       cTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCg     >  2:6342463/1‑93 (MQ=255)
                                                                                       cTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCg     <  1:3747643/93‑1 (MQ=255)
                                                                                       cTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCg     <  1:2899615/93‑1 (MQ=255)
                                                                                        tCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGc    >  2:4070412/1‑93 (MQ=255)
                                                                                          aaG‑AAAAAGTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCt  >  1:6493618/1‑93 (MQ=255)
                                                                                             |                                                                                          
TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT  >  NC_000913/3099564‑3099745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  NC_000913/3099570‑3099754
                                                                                            |                                                                                              
TGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAat                                                                                              >  SRR4302547.1088870/1‑92 (MQ=60)
 GCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAatc                                                                                             <  SRR4302547.2402921/94‑4 (MQ=60)
   ACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGTAAAAA‑TCAG                                                                                           <  SRR4302547.6593252/94‑1 (MQ=60)
    CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGG                                                                                          <  SRR4302547.51976/94‑1 (MQ=60)
    CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGG                                                                                          <  SRR4302547.6341447/94‑1 (MQ=60)
     TGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGC                                                                                         <  SRR4302547.182441/94‑1 (MQ=60)
     TGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAatcatac                                                                                         >  SRR4302547.1016683/1‑87 (MQ=60)
       GTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCA                                                                                       <  SRR4302547.4843536/94‑1 (MQ=60)
       GTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCA                                                                                       >  SRR4302547.5220006/1‑94 (MQ=60)
        TAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAG                                                                                      <  SRR4302547.1550239/94‑1 (MQ=60)
         AGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGC                                                                                     >  SRR4302547.4175798/1‑94 (MQ=60)
         AGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGC                                                                                     >  SRR4302547.142940/1‑94 (MQ=60)
           TCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCAT                                                                                   >  SRR4302547.4448426/1‑94 (MQ=60)
           TCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCAT                                                                                   <  SRR4302547.5916747/94‑1 (MQ=60)
             GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTT                                                                                 <  SRR4302547.4716374/94‑1 (MQ=60)
              GCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTG                                                                                <  SRR4302547.1040959/94‑1 (MQ=60)
               CGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGC                                                                               <  SRR4302547.1683017/94‑1 (MQ=60)
                GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCT                                                                              >  SRR4302547.5205547/1‑94 (MQ=60)
                GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCT                                                                              <  SRR4302547.4306959/94‑1 (MQ=60)
                GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGACAGCATTTGCT                                                                              >  SRR4302547.1218559/1‑94 (MQ=60)
                 TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTG                                                                             <  SRR4302547.5875820/94‑1 (MQ=60)
                 TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTG                                                                             >  SRR4302547.1721048/1‑94 (MQ=60)
                 TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTG                                                                             >  SRR4302547.3297308/1‑94 (MQ=60)
                 TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTG                                                                             <  SRR4302547.455142/94‑1 (MQ=60)
                 GGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTG                                                                             <  SRR4302547.6667884/94‑1 (MQ=60)
                 GGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTG                                                                             <  SRR4302547.1031350/94‑1 (MQ=60)
                  GAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGG                                                                            <  SRR4302547.1919538/94‑1 (MQ=60)
                  GAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGG                                                                            <  SRR4302547.345242/94‑1 (MQ=60)
                   AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGC                                                                           >  SRR4302547.476823/1‑94 (MQ=60)
                   AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGC                                                                           <  SRR4302547.2169965/94‑1 (MQ=60)
                    GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCC                                                                          <  SRR4302547.6779547/94‑1 (MQ=60)
                    GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCC                                                                          <  SRR4302547.3002691/94‑1 (MQ=60)
                     CGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCC                                                                         <  SRR4302547.4237565/94‑1 (MQ=60)
                     CGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCC                                                                         >  SRR4302547.3007293/1‑94 (MQ=60)
                     CGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCC                                                                         <  SRR4302547.5474426/94‑1 (MQ=60)
                      GGTCATGGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCC                                                                        <  SRR4302547.1822074/94‑1 (MQ=60)
                         CATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGA                                                                     >  SRR4302547.6736727/1‑94 (MQ=60)
                          ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    <  SRR4302547.5259530/94‑1 (MQ=60)
                           TTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATT                                                                   <  SRR4302547.884131/94‑1 (MQ=60)
                            TGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTG                                                                  >  SRR4302547.3429122/1‑94 (MQ=60)
                              CCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGAT                                                                <  SRR4302547.538686/94‑1 (MQ=60)
                              CCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTATTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGAT                                                                >  SRR4302547.4818217/1‑94 (MQ=60)
                               CGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATA                                                               >  SRR4302547.3042994/1‑94 (MQ=60)
                               CGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATA                                                               <  SRR4302547.4402502/94‑1 (MQ=60)
                                 TCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAAC                                                             <  SRR4302547.6192277/94‑1 (MQ=60)
                                  CACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACA                                                            <  SRR4302547.6654456/94‑1 (MQ=60)
                                   ACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAA                                                           >  SRR4302547.6410906/1‑94 (MQ=60)
                                   ACGGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAA                                                           >  SRR4302547.5383571/1‑94 (MQ=60)
                                     TGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAG                                                         >  SRR4302547.3309073/1‑94 (MQ=60)
                                     TGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAG                                                         <  SRR4302547.5872664/94‑1 (MQ=60)
                                     TGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAG                                                         >  SRR4302547.4436112/1‑94 (MQ=60)
                                      GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        >  SRR4302547.6476117/1‑94 (MQ=60)
                                      GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302547.18829/94‑1 (MQ=60)
                                        AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                      <  SRR4302547.5831412/94‑1 (MQ=60)
                                        AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                      <  SRR4302547.2518132/94‑1 (MQ=60)
                                           TGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCG                                                   <  SRR4302547.552920/94‑1 (MQ=60)
                                            CCTGCCGCGAGCAGCATTTTGCGCATCATAGTCTTCCCTAAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGT                                                  <  SRR4302547.3480464/94‑1 (MQ=60)
                                             CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                 <  SRR4302547.2578651/94‑1 (MQ=60)
                                             CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                 <  SRR4302547.2062687/94‑1 (MQ=60)
                                              TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                >  SRR4302547.776338/1‑94 (MQ=60)
                                               GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                               >  SRR4302547.150315/1‑94 (MQ=60)
                                                 CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                             >  SRR4302547.2409067/1‑94 (MQ=60)
                                                  GCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCA                                            <  SRR4302547.1140578/94‑1 (MQ=60)
                                                  GCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCA                                            >  SRR4302547.2813499/1‑94 (MQ=60)
                                                  GCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCA                                            <  SRR4302547.5770914/94‑1 (MQ=60)
                                                   CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302547.2743468/94‑1 (MQ=60)
                                                   CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302547.1755612/94‑1 (MQ=60)
                                                    CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          <  SRR4302547.1615540/94‑1 (MQ=60)
                                                    CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          >  SRR4302547.4087172/1‑94 (MQ=60)
                                                    CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          <  SRR4302547.4504714/94‑1 (MQ=60)
                                                    CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          <  SRR4302547.475429/94‑1 (MQ=60)
                                                    CAGCAGCATTTTGCGCATCATAGTCTCCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          <  SRR4302547.4680630/94‑1 (MQ=60)
                                                     AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                         <  SRR4302547.3899614/94‑1 (MQ=60)
                                                     AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                         >  SRR4302547.5280130/1‑94 (MQ=60)
                                                     AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                         <  SRR4302547.2313089/94‑1 (MQ=60)
                                                      GCAGTATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                        <  SRR4302547.1909927/94‑1 (MQ=60)
                                                      GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                        >  SRR4302547.1375847/1‑94 (MQ=60)
                                                      GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                        <  SRR4302547.4342796/94‑1 (MQ=60)
                                                       CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       <  SRR4302547.6381391/94‑1 (MQ=60)
                                                       CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       >  SRR4302547.6010308/1‑94 (MQ=60)
                                                       CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       <  SRR4302547.4462426/94‑1 (MQ=60)
                                                       CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       <  SRR4302547.6506878/94‑1 (MQ=60)
                                                        AGCATTTTGCGCATCATAGTGTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      <  SRR4302547.4775320/94‑1 (MQ=60)
                                                        AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      >  SRR4302547.1973019/1‑94 (MQ=60)
                                                        AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      <  SRR4302547.1614058/94‑1 (MQ=60)
                                                        AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      >  SRR4302547.1460436/1‑94 (MQ=60)
                                                         GCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGA                                     >  SRR4302547.5055169/1‑94 (MQ=60)
                                                         GCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGA                                     <  SRR4302547.4081605/94‑1 (MQ=60)
                                                          CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    <  SRR4302547.2628807/94‑1 (MQ=60)
                                                          CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    <  SRR4302547.6159131/94‑1 (MQ=60)
                                                          CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302547.4127803/1‑94 (MQ=60)
                                                          CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302547.6201304/1‑94 (MQ=60)
                                                          CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302547.957898/1‑94 (MQ=60)
                                                          CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    <  SRR4302547.3747161/94‑1 (MQ=60)
                                                          CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302547.2206720/1‑94 (MQ=60)
                                                           ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   >  SRR4302547.501195/1‑94 (MQ=60)
                                                           ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   >  SRR4302547.1422299/1‑94 (MQ=60)
                                                           ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   >  SRR4302547.4395049/1‑94 (MQ=60)
                                                            TTTTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGC                                  <  SRR4302547.2803411/94‑1 (MQ=60)
                                                             TTCGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCG                                 <  SRR4302547.786676/94‑1 (MQ=60)
                                                              TTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGG                                <  SRR4302547.5189017/94‑1 (MQ=60)
                                                              TTGCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGG                                <  SRR4302547.3034218/94‑1 (MQ=60)
                                                                GCGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                              <  SRR4302547.2045677/94‑1 (MQ=60)
                                                                 CGCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAGAGTACGCGTTTTCCATGCCGGATGCGGCGT                             >  SRR4302547.560066/1‑94 (MQ=60)
                                                                  GCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTA                            >  SRR4302547.2301938/1‑94 (MQ=60)
                                                                  GCATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTA                            >  SRR4302547.2829134/1‑94 (MQ=60)
                                                                    ATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAC                          >  SRR4302547.5234657/1‑94 (MQ=60)
                                                                    ATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                          >  SRR4302547.3392874/1‑94 (MQ=60)
                                                                    ATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                          >  SRR4302547.1627095/1‑94 (MQ=60)
                                                                    ATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                          >  SRR4302547.6498009/1‑94 (MQ=60)
                                                                    ATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                          <  SRR4302547.3322141/94‑1 (MQ=60)
                                                                    ATCATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAA                          >  SRR4302547.508272/1‑94 (MQ=60)
                                                                      CATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACA                        <  SRR4302547.2648606/94‑1 (MQ=60)
                                                                      CATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACA                        >  SRR4302547.3787216/1‑94 (MQ=60)
                                                                      CATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACA                        >  SRR4302547.3943307/1‑94 (MQ=60)
                                                                       ATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAC                       <  SRR4302547.2420424/94‑1 (MQ=60)
                                                                       ATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAC                       <  SRR4302547.5673515/94‑1 (MQ=60)
                                                                       ATAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAC                       >  SRR4302547.1921120/1‑94 (MQ=60)
                                                                        TAGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACC                      >  SRR4302547.4990335/1‑94 (MQ=60)
                                                                         AGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT                     <  SRR4302547.5437503/94‑1 (MQ=60)
                                                                         AGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT                     <  SRR4302547.312510/94‑1 (MQ=60)
                                                                         AGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT                     <  SRR4302547.3168879/94‑1 (MQ=60)
                                                                         AGTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT                     <  SRR4302547.4922245/94‑1 (MQ=60)
                                                                          GTCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTT                    <  SRR4302547.5651271/94‑1 (MQ=60)
                                                                           TCTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTA                   <  SRR4302547.6660480/94‑1 (MQ=60)
                                                                            CTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                  <  SRR4302547.579803/94‑1 (MQ=60)
                                                                            CTTCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTAT                  >  SRR4302547.6521365/1‑94 (MQ=60)
                                                                              TCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCC                <  SRR4302547.2589838/94‑1 (MQ=60)
                                                                              TCCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCC                >  SRR4302547.6069025/1‑94 (MQ=60)
                                                                               CCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCC               >  SRR4302547.972738/1‑94 (MQ=60)
                                                                               CCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCC               >  SRR4302547.2118353/1‑94 (MQ=60)
                                                                               CCCTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCC               >  SRR4302547.5575807/1‑94 (MQ=60)
                                                                                CCTCAAGAAAAAA‑TCAGGCCAGCATGTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCG              <  SRR4302547.1470482/94‑1 (MQ=60)
                                                                                 CTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC             <  SRR4302547.2957438/94‑1 (MQ=60)
                                                                                 CTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC             <  SRR4302547.3824145/94‑1 (MQ=60)
                                                                                 CTCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC             >  SRR4302547.4565494/1‑94 (MQ=60)
                                                                                  TCAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCC            >  SRR4302547.5797333/1‑94 (MQ=60)
                                                                                   CAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT           >  SRR4302547.1933492/1‑94 (MQ=60)
                                                                                   CAAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT           >  SRR4302547.2229448/1‑94 (MQ=60)
                                                                                   CAAGAAAAAA‑TCAGGACAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT           >  SRR4302547.2893873/1‑94 (MQ=60)
                                                                                   CAAG‑AAAAAGTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCT           >  SRR4302547.6628799/1‑94 (MQ=60)
                                                                                    AAGAAAAAA‑TCAGGCCAGCATTTGGTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA          <  SRR4302547.2444501/94‑1 (MQ=60)
                                                                                    AAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA          >  SRR4302547.1960302/1‑94 (MQ=60)
                                                                                    AAGAAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTA          >  SRR4302547.2320177/1‑94 (MQ=60)
                                                                                     agaAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTAC         >  SRR4302547.5712107/4‑94 (MQ=60)
                                                                                      gaAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT        >  SRR4302547.140793/3‑94 (MQ=60)
                                                                                      gaAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT        <  SRR4302547.595133/92‑1 (MQ=60)
                                                                                      gaAAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACT        >  SRR4302547.6265858/3‑94 (MQ=60)
                                                                                        AAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCG      <  SRR4302547.4222971/94‑1 (MQ=60)
                                                                                          AAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAT    <  SRR4302547.2622532/94‑1 (MQ=60)
                                                                                          AAA‑GCAGGCCAGCATTGGCTGGCCCCAGATTGATAACAAAGTACGGGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGAT    <  SRR4302547.933835/94‑1 (MQ=60)
                                                                                           AA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATC   <  SRR4302547.4860504/94‑1 (MQ=60)
                                                                                           AA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGAGTAAACACCTTATCCCGCCTACTCGATC   >  SRR4302547.4734692/1‑94 (MQ=60)
                                                                                            A‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  SRR4302547.3211869/1‑94 (MQ=60)
                                                                                            A‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCATAAACACCTTATCCCGCCTACTCGATCG  <  SRR4302547.5220710/94‑1 (MQ=60)
                                                                                            |                                                                                              
TGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA‑TCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  NC_000913/3099570‑3099754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑