Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,115,116 +C coding (2013/2028 nt) b2974 ← putative endoglucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,115,1151.C99.5% 807.4 / ‑6.2 219K672E (AAA→GAA) b2974putative endoglucanase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (97/121);  minor base A (1/0);  total (98/121)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115023‑3115202
                                                                                             |                                                                                       
   tGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cct                                                                                      <  1:1604758/93‑3 (MQ=255)
   tGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cct                                                                                      >  1:3420181/1‑91 (MQ=255)
    tcTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cctg                                                                                     <  1:2270734/92‑4 (MQ=255)
    gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cctg                                                                                     >  1:152325/1‑90 (MQ=255)
     cTGGTATCTGCACGCAGCGGGATCGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑cctgc                                                                                    <  1:3502165/93‑5 (MQ=255)
       ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCgg                                                                                   >  1:4801016/1‑93 (MQ=255)
        gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGa                                                                                  <  2:5859512/93‑1 (MQ=255)
        gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGa                                                                                  >  1:4147/1‑93 (MQ=255)
        gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGa                                                                                  <  1:6064871/93‑1 (MQ=255)
         tATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGAt                                                                                 <  1:13272/93‑1 (MQ=255)
           tCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCg                                                                               <  2:3043382/93‑1 (MQ=255)
           tCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCg                                                                               <  2:5155795/93‑1 (MQ=255)
            cTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCga                                                                              <  1:1966801/93‑1 (MQ=255)
              gCACGCAGGGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGACATTTCCTGCGGATCgaga                                                                            <  2:3737057/93‑1 (MQ=255)
              gCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCgaga                                                                            <  2:1093370/93‑1 (MQ=255)
              gCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCgaga                                                                            <  2:1437095/93‑1 (MQ=255)
                 cGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCg                                                                         >  1:5208000/1‑93 (MQ=255)
                  gCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGc                                                                        <  2:2665139/93‑1 (MQ=255)
                    aGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCa                                                                      <  2:666313/93‑1 (MQ=255)
                     gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCAt                                                                     <  1:4087405/93‑1 (MQ=255)
                      cGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATa                                                                    <  2:4816208/93‑1 (MQ=255)
                       gggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAg                                                                   >  1:333918/1‑93 (MQ=255)
                        ggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGc                                                                  <  2:3759402/93‑1 (MQ=255)
                         gATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCt                                                                 >  2:5132714/1‑93 (MQ=255)
                          aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTa                                                                <  1:539911/93‑1 (MQ=255)
                           ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCTAGATCGCCATAGCTAc                                                               <  2:4988313/93‑1 (MQ=255)
                            tGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACt                                                              <  1:184263/93‑1 (MQ=255)
                            tGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACt                                                              <  1:5920617/93‑1 (MQ=255)
                             gCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACtt                                                             <  2:2335010/93‑1 (MQ=255)
                             gCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACtt                                                             >  2:5818971/1‑93 (MQ=255)
                             gCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACtt                                                             >  1:5188061/1‑93 (MQ=255)
                              cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                            <  1:1827648/93‑1 (MQ=255)
                              cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                            <  1:4377703/93‑1 (MQ=255)
                               aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  2:1687076/1‑93 (MQ=255)
                               aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  1:1176955/1‑93 (MQ=255)
                               aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           <  1:16589/93‑1 (MQ=255)
                                tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          <  2:709376/93‑1 (MQ=255)
                                tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  1:2692716/1‑93 (MQ=255)
                                tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  1:833899/1‑93 (MQ=255)
                                  aGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATg                                                        <  2:5294586/93‑1 (MQ=255)
                                   ggATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                       <  2:1851210/93‑1 (MQ=255)
                                   ggATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                       <  1:5351790/93‑1 (MQ=255)
                                     aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  2:1557590/93‑1 (MQ=255)
                                     aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  1:3654779/93‑1 (MQ=255)
                                       cGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCaa                                                   <  1:2525207/93‑1 (MQ=255)
                                       cGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCaa                                                   <  1:2076887/93‑1 (MQ=255)
                                        gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                  <  2:1268058/93‑1 (MQ=255)
                                         ttACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACa                                                 >  2:1358834/1‑93 (MQ=255)
                                          tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                <  2:3158410/93‑1 (MQ=255)
                                           aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               >  1:3227669/1‑93 (MQ=255)
                                            cccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACagag                                              <  2:4756384/93‑1 (MQ=255)
                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             <  2:6007096/93‑1 (MQ=255)
                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             <  2:2218961/93‑1 (MQ=255)
                                             ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             <  2:360543/93‑1 (MQ=255)
                                              cACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAt                                            >  1:2822847/1‑93 (MQ=255)
                                                ccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                          <  1:2367335/93‑1 (MQ=255)
                                                  tGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                        >  1:1012198/1‑93 (MQ=255)
                                                  tGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                        >  2:5644701/1‑93 (MQ=255)
                                                        acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  <  1:3996233/93‑1 (MQ=255)
                                                         caTATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAt                                 >  1:4906797/1‑93 (MQ=255)
                                                          atatTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATc                                <  1:4201468/93‑1 (MQ=255)
                                                           tatTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATcc                               >  2:4491228/1‑93 (MQ=255)
                                                            atTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCg                              >  1:3720614/1‑93 (MQ=255)
                                                              tCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGa                            >  2:5423982/1‑93 (MQ=255)
                                                               cAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAt                           <  2:3232411/93‑1 (MQ=255)
                                                               cAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAt                           <  2:4143970/93‑1 (MQ=255)
                                                                aaaGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATg                          <  2:3293822/93‑1 (MQ=255)
                                                                aaaGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATg                          >  2:3937998/1‑93 (MQ=255)
                                                                aaaGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATg                          <  1:2143253/93‑1 (MQ=255)
                                                                 aaGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                         >  2:1962579/1‑93 (MQ=255)
                                                                 aaGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                         >  2:1883056/1‑93 (MQ=255)
                                                                   gCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                       >  2:5234488/1‑93 (MQ=255)
                                                                   gCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                       >  1:4262272/1‑93 (MQ=255)
                                                                   gCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                       <  1:381060/93‑1 (MQ=255)
                                                                     cGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGCTGCCCGCAAAATCCGGATGGAAGt                     <  2:4387954/93‑1 (MQ=255)
                                                                      gTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    >  2:1724469/1‑93 (MQ=255)
                                                                      gTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    >  1:452058/1‑93 (MQ=255)
                                                                      gTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    <  2:1134492/93‑1 (MQ=255)
                                                                      gTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    <  1:5705760/93‑1 (MQ=255)
                                                                       ttAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  2:6101261/1‑93 (MQ=255)
                                                                       ttAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  1:700015/1‑93 (MQ=255)
                                                                         aaGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                 <  1:2802864/93‑1 (MQ=255)
                                                                         aaGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                 >  1:4667187/1‑93 (MQ=255)
                                                                            gATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGc              <  1:905439/93‑1 (MQ=255)
                                                                             aTCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  1:743053/93‑1 (MQ=255)
                                                                             aTCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  1:4615126/93‑1 (MQ=255)
                                                                              tCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAg            <  1:3099647/93‑1 (MQ=255)
                                                                              tCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAg            >  2:2804318/1‑93 (MQ=255)
                                                                              tCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAg            >  2:1081312/1‑93 (MQ=255)
                                                                               cagcagCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc           <  1:4866018/93‑1 (MQ=255)
                                                                               cagcagCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc           >  2:4620974/1‑93 (MQ=255)
                                                                                 gcagcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgc         <  2:2981998/93‑1 (MQ=255)
                                                                                 gcagcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgc         <  1:3580422/93‑1 (MQ=255)
                                                                                 gcagcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgc         >  2:966259/1‑93 (MQ=255)
                                                                                  cagcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt        >  2:3004197/1‑93 (MQ=255)
                                                                                   agcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTg       >  2:1337669/1‑93 (MQ=255)
                                                                                    gcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt      >  2:1920985/1‑93 (MQ=255)
                                                                                    gcGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt      <  2:905634/93‑1 (MQ=255)
                                                                                       gCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCGGCGCTGTTTc   <  2:1458177/93‑1 (MQ=255)
                                                                                        cATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTcc  >  2:2387407/1‑93 (MQ=255)
                                                                                             |                                                                                       
GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115023‑3115202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  NC_000913/3115023‑3115209
                                                                                              |                                                                                             
GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑C                                                                                               >  SRR4302532.2590282/1‑94 (MQ=60)
 TTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CC                                                                                              <  SRR4302532.6140656/94‑1 (MQ=60)
 TTTGCTGGTATCTGCACGCAGCGGGACTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CC                                                                                              <  SRR4302532.2010585/94‑1 (MQ=60)
  TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cct                                                                                             >  SRR4302532.3534754/1‑92 (MQ=60)
  TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cct                                                                                             >  SRR4302532.3534755/1‑92 (MQ=60)
   TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cctg                                                                                            >  SRR4302532.2049870/1‑91 (MQ=60)
   TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cctg                                                                                            >  SRR4302532.157419/1‑91 (MQ=60)
   TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cctg                                                                                            <  SRR4302532.1659081/94‑4 (MQ=60)
    TCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGC                                                                                            <  SRR4302532.2346091/94‑1 (MQ=60)
     CTGGTATCTGCACGCAGCGGGATCGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCG                                                                                           <  SRR4302532.3619477/94‑1 (MQ=60)
      TGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGG                                                                                          >  SRR4302532.4966358/1‑94 (MQ=60)
       GGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGA                                                                                         >  SRR4302532.4319/1‑94 (MQ=60)
        GTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGAT                                                                                        <  SRR4302532.6274309/94‑1 (MQ=60)
         TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATC                                                                                       <  SRR4302532.13818/94‑1 (MQ=60)
         TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATC                                                                                       >  SRR4302532.4654642/1‑94 (MQ=60)
          ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCG                                                                                      >  SRR4302532.2861846/1‑94 (MQ=60)
          ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCG                                                                                      <  SRR4302532.4820238/94‑1 (MQ=60)
           TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGA                                                                                     >  SRR4302532.3640077/1‑94 (MQ=60)
            CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAG                                                                                    <  SRR4302532.2032132/94‑1 (MQ=60)
            CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAG                                                                                    <  SRR4302532.5216839/94‑1 (MQ=60)
              GCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGAT                                                                                  <  SRR4302532.1852259/94‑1 (MQ=60)
               CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATC                                                                                 <  SRR4302532.5756371/94‑1 (MQ=60)
               CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATC                                                                                 <  SRR4302532.5517193/94‑1 (MQ=60)
                ACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCG                                                                                <  SRR4302532.3803665/94‑1 (MQ=60)
                ACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCG                                                                                >  SRR4302532.5387604/1‑94 (MQ=60)
                  GCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCC                                                                              >  SRR4302532.6000020/1‑94 (MQ=60)
                  GCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCC                                                                              >  SRR4302532.3602589/1‑94 (MQ=60)
                    AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCAT                                                                            <  SRR4302532.1654934/94‑1 (MQ=60)
                     GCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATA                                                                           <  SRR4302532.4224608/94‑1 (MQ=60)
                      CGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGGATTTCCTGCGGATCGAGATCGCCATAC                                                                          >  SRR4302532.157411/1‑94 (MQ=60)
                      CGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAG                                                                          >  SRR4302532.345495/1‑94 (MQ=60)
                        GGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCT                                                                        >  SRR4302532.4731347/1‑94 (MQ=60)
                         GATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTA                                                                       <  SRR4302532.5563008/94‑1 (MQ=60)
                          ATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                      <  SRR4302532.558701/94‑1 (MQ=60)
                           TTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACT                                                                     <  SRR4302532.1790241/94‑1 (MQ=60)
                            TGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTT                                                                    <  SRR4302532.190476/94‑1 (MQ=60)
                            TGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTT                                                                    <  SRR4302532.6125646/94‑1 (MQ=60)
                            TGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTT                                                                    >  SRR4302532.5366849/1‑94 (MQ=60)
                            TGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTT                                                                    >  SRR4302532.2253528/1‑94 (MQ=60)
                              CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAA                                                                  <  SRR4302532.4526348/94‑1 (MQ=60)
                              CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAA                                                                  >  SRR4302532.1216278/1‑94 (MQ=60)
                              CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAA                                                                  <  SRR4302532.1888483/94‑1 (MQ=60)
                              CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGCTAAGGATCAGCAGCGGCATTTCCTGCGGATCCAGATCGCCATCACTACTTAA                                                                  >  SRR4302532.102565/1‑94 (MQ=60)
                               ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302532.862488/1‑94 (MQ=60)
                               ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302532.2783161/1‑94 (MQ=60)
                               ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 <  SRR4302532.4605715/94‑1 (MQ=60)
                               ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 <  SRR4302532.17265/94‑1 (MQ=60)
                               ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302532.862487/1‑94 (MQ=60)
                                TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                <  SRR4302532.4319468/94‑1 (MQ=60)
                                 AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATG                                                               <  SRR4302532.4007810/94‑1 (MQ=60)
                                   GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                             <  SRR4302532.5537414/94‑1 (MQ=60)
                                   GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                             <  SRR4302532.2364310/94‑1 (MQ=60)
                                    GATCGTTACCCACCTGAGTCACATATTCAAAGCCGTT‑AGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           >  SRR4302532.2386723/1‑94 (MQ=60)
                                     ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           <  SRR4302532.3777478/94‑1 (MQ=60)
                                     ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           <  SRR4302532.5438170/94‑1 (MQ=60)
                                     ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           >  SRR4302532.3326651/1‑94 (MQ=60)
                                      TCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAA                                                          <  SRR4302532.4898014/94‑1 (MQ=60)
                                       CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                         <  SRR4302532.2146120/94‑1 (MQ=60)
                                       CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                         <  SRR4302532.2609459/94‑1 (MQ=60)
                                         TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                       >  SRR4302532.4896034/1‑94 (MQ=60)
                                          TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      <  SRR4302532.3753756/94‑1 (MQ=60)
                                          TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      <  SRR4302532.2062548/94‑1 (MQ=60)
                                          TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      <  SRR4302532.3521346/94‑1 (MQ=60)
                                          TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      >  SRR4302532.3334799/1‑94 (MQ=60)
                                          TACCCACCTGAGGCAGAGAGTCAAAGCCGGTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      >  SRR4302532.5606089/1‑94 (MQ=60)
                                           ACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAG                                                     >  SRR4302532.2846858/1‑94 (MQ=60)
                                            CCCACCTGAGTCACA‑ATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302532.1356338/94‑1 (MQ=60)
                                             CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   >  SRR4302532.2917300/1‑94 (MQ=60)
                                              CACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATG                                                  >  SRR4302532.331235/1‑94 (MQ=60)
                                                CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                <  SRR4302532.2446282/94‑1 (MQ=60)
                                                 CTGAGTCACATATTCAAGGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               >  SRR4302532.4308390/1‑94 (MQ=60)
                                                 CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               >  SRR4302532.1046591/1‑94 (MQ=60)
                                                 CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               >  SRR4302532.1902873/1‑94 (MQ=60)
                                                  TGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCG                                              <  SRR4302532.1560026/94‑1 (MQ=60)
                                                   GAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGC                                             >  SRR4302532.3423266/1‑94 (MQ=60)
                                                   GAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGC                                             >  SRR4302532.2913880/1‑94 (MQ=60)
                                                    AGACACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCA                                            >  SRR4302532.6319613/1‑94 (MQ=60)
                                                     GTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAA                                           >  SRR4302532.998691/1‑94 (MQ=60)
                                                      TCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                          <  SRR4302532.747139/94‑1 (MQ=60)
                                                      TCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                          >  SRR4302532.4257762/1‑94 (MQ=60)
                                                      TCACATATTCAAAGCAGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                          <  SRR4302532.3143667/94‑1 (MQ=60)
                                                        ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        <  SRR4302532.4130854/94‑1 (MQ=60)
                                                        ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        >  SRR4302532.5075397/1‑94 (MQ=60)
                                                          ATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCC                                      >  SRR4302532.4476223/1‑94 (MQ=60)
                                                          ATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCC                                      <  SRR4302532.4342504/94‑1 (MQ=60)
                                                           TATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                     >  SRR4302532.3845524/1‑94 (MQ=60)
                                                              TCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAT                                  >  SRR4302532.3726293/1‑94 (MQ=60)
                                                               CAAAGCCTTTAAGGATCAGCAGCTGCATTTCCTGCGGATCGAGATCGCCATGGCTACTTAAATGCTCCACAGAGGTGCCCGCAAACTCCGGATG                                 >  SRR4302532.6328148/1‑94 (MQ=60)
                                                                AAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGG                                <  SRR4302532.2214840/94‑1 (MQ=60)
                                                                  AGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAA                              >  SRR4302532.4405686/1‑94 (MQ=60)
                                                                   GCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             <  SRR4302532.394359/94‑1 (MQ=60)
                                                                    CCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                            <  SRR4302532.5371485/94‑1 (MQ=60)
                                                                     CGTTAAGGATCAGCAGCGGCGTTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCATCAGAGATGCCCGCAAAATCCGGATGGAAGTC                           <  SRR4302532.3920380/94‑1 (MQ=60)
                                                                     CGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           >  SRR4302532.467772/1‑94 (MQ=60)
                                                                      gatctcGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302532.5242780/7‑94 (MQ=60)
                                                                      GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          <  SRR4302532.5904695/94‑1 (MQ=60)
                                                                      GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302532.723959/1‑94 (MQ=60)
                                                                        TAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAA                        >  SRR4302532.4826306/1‑94 (MQ=60)
                                                                         AAGGATCAGCAGCGGCATTTCCTTCGGATCGAGATCGCCATAGCTACTTAAATTCTCAACAGAGATGCCCGCAAAACCCGGATGGAAGTCGAAC                       <  SRR4302532.1455060/94‑1 (MQ=60)
                                                                         AAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                       <  SRR4302532.2896769/94‑1 (MQ=60)
                                                                          AGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACG                      <  SRR4302532.4167413/94‑1 (MQ=60)
                                                                            GATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCA                    <  SRR4302532.936349/94‑1 (MQ=60)
                                                                             ATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   <  SRR4302532.768452/94‑1 (MQ=60)
                                                                             ATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   <  SRR4302532.4772489/94‑1 (MQ=60)
                                                                             ATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   >  SRR4302532.895978/1‑94 (MQ=60)
                                                                              TCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGC                  <  SRR4302532.6086310/94‑1 (MQ=60)
                                                                              TCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGC                  <  SRR4302532.3202436/94‑1 (MQ=60)
                                                                               CAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCG                 <  SRR4302532.5033365/94‑1 (MQ=60)
                                                                                 GCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCT               <  SRR4302532.3700196/94‑1 (MQ=60)
                                                                                  CAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTG              >  SRR4302532.2713341/1‑94 (MQ=60)
                                                                                  CAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTG              >  SRR4302532.5108022/1‑94 (MQ=60)
                                                                                   AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTGAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT             >  SRR4302532.4367768/1‑94 (MQ=60)
                                                                                   AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT             >  SRR4302532.503113/1‑94 (MQ=60)
                                                                                   AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT             >  SRR4302532.405100/1‑94 (MQ=60)
                                                                                    GCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTT            <  SRR4302532.2185705/94‑1 (MQ=60)
                                                                                    GCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTT            >  SRR4302532.5040290/1‑94 (MQ=60)
                                                                                     CGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTT           <  SRR4302532.727308/94‑1 (MQ=60)
                                                                                       GCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC         <  SRR4302532.167565/94‑1 (MQ=60)
                                                                                        CATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCT        <  SRR4302532.1089142/94‑1 (MQ=60)
                                                                                         ATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTG       >  SRR4302532.653154/1‑94 (MQ=60)
                                                                                          TTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTA     <  SRR4302532.4043704/94‑1 (MQ=60)
                                                                                           TC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAA    <  SRR4302532.2694176/94‑1 (MQ=60)
                                                                                              CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  SRR4302532.3457964/1‑94 (MQ=60)
                                                                                              CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  SRR4302532.5530480/1‑94 (MQ=60)
                                                                                              |                                                                                             
GTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  NC_000913/3115023‑3115209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑