Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,115,116 +C coding (2013/2028 nt) b2974 ← putative endoglucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,115,1151.C99.6% 957.3 / ‑3.8 257coding (2014/2028 nt)b2974putative endoglucanase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (131/125);  total (132/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

Only 100 of 271 total aligned reads displayed.

TTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115024‑3115202
                                                                                             |                                                                                       
 ttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cc                                                                                       <  2:6337275/93‑2 (MQ=255)
  tGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cct                                                                                      <  1:5388371/93‑3 (MQ=255)
   gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cctg                                                                                     <  1:4170282/93‑4 (MQ=255)
      ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCgg                                                                                   >  2:3813415/1‑93 (MQ=255)
        tATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGAt                                                                                 <  1:5846327/93‑1 (MQ=255)
         aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATc                                                                                <  1:4973553/93‑1 (MQ=255)
         aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATc                                                                                <  1:1703832/93‑1 (MQ=255)
          tCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCg                                                                               <  1:5138637/93‑1 (MQ=255)
          tCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCg                                                                               <  1:6075119/93‑1 (MQ=255)
          tCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCg                                                                               >  2:1372880/1‑93 (MQ=255)
           cTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCga                                                                              <  1:1523576/93‑1 (MQ=255)
           cTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCga                                                                              <  1:2456962/93‑1 (MQ=255)
            tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCgag                                                                             >  1:5363728/1‑93 (MQ=255)
            tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGCCACATATTCAAAGCCGTTAAGGATCACCAGCGGCAT‑TTCCTGCGGATCgcg                                                                             >  1:6353817/1‑91 (MQ=255)
             gCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCgaga                                                                            >  2:2667461/1‑93 (MQ=255)
             gCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCgaga                                                                            <  2:4508617/93‑1 (MQ=255)
              cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGAt                                                                           <  1:1232819/93‑1 (MQ=255)
              cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGAt                                                                           <  2:5551323/93‑1 (MQ=255)
              cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGAt                                                                           <  1:852468/93‑1 (MQ=255)
              cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGAt                                                                           >  2:4462757/1‑93 (MQ=255)
               aCGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                          >  1:4225357/1‑93 (MQ=255)
                 gCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGc                                                                        >  1:5768853/1‑93 (MQ=255)
                    gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCAt                                                                     >  2:882036/1‑93 (MQ=255)
                    gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCAt                                                                     >  1:2807558/1‑93 (MQ=255)
                     ggggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATa                                                                    <  2:2528599/92‑1 (MQ=255)
                     cGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATa                                                                    >  2:5750427/1‑93 (MQ=255)
                       ggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGc                                                                  >  2:2204235/1‑93 (MQ=255)
                        gATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCt                                                                 >  2:1621031/1‑93 (MQ=255)
                         aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTa                                                                <  2:4638137/93‑1 (MQ=255)
                          ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTat                                                               >  2:5350910/1‑92 (MQ=255)
                           tGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACt                                                              >  1:4390215/1‑93 (MQ=255)
                              aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  2:393246/1‑93 (MQ=255)
                              aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  2:4529450/1‑93 (MQ=255)
                              aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                           >  2:3508188/1‑93 (MQ=255)
                               tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  2:4139551/1‑93 (MQ=255)
                               tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  1:1941067/1‑93 (MQ=255)
                               tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  2:6472628/1‑93 (MQ=255)
                               tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  2:3311450/1‑93 (MQ=255)
                               tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  1:3269977/1‑93 (MQ=255)
                                aaGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAAt                                                         <  2:2942726/93‑1 (MQ=255)
                                 aGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATg                                                        >  1:229428/1‑93 (MQ=255)
                                  ggATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                       <  1:6554137/93‑1 (MQ=255)
                                   gATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCt                                                      >  2:4064570/1‑93 (MQ=255)
                                    aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  1:3315865/93‑1 (MQ=255)
                                     acGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCa                                                    <  2:760417/92‑1 (MQ=255)
                                      cGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCaa                                                   >  1:5617687/1‑93 (MQ=255)
                                      cGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCaa                                                   <  1:2835368/93‑1 (MQ=255)
                                        ttACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACa                                                 >  1:3799420/1‑93 (MQ=255)
                                        ttACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACa                                                 >  2:730077/1‑93 (MQ=255)
                                         tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                <  2:4762757/93‑1 (MQ=255)
                                         tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                <  1:1104162/93‑1 (MQ=255)
                                         tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                <  1:5318126/93‑1 (MQ=255)
                                         tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGAAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                >  2:2767700/1‑93 (MQ=255)
                                          aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               >  1:1434559/1‑93 (MQ=255)
                                          aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               <  2:1428426/93‑1 (MQ=255)
                                          aCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                               >  2:546305/1‑93 (MQ=255)
                                              cccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATg                                           <  1:5963216/92‑1 (MQ=255)
                                              aCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATg                                           <  1:6180107/93‑1 (MQ=255)
                                                cTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                         <  2:131331/93‑1 (MQ=255)
                                                 tGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                        <  2:6607570/93‑1 (MQ=255)
                                                    gTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCa                                     <  1:3627115/93‑1 (MQ=255)
                                                    gTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCa                                     >  2:4178644/1‑93 (MQ=255)
                                                     tCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaa                                    <  1:496679/93‑1 (MQ=255)
                                                     tCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaa                                    <  1:5442336/93‑1 (MQ=255)
                                                       acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  <  2:6059643/93‑1 (MQ=255)
                                                       acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  <  1:2949919/93‑1 (MQ=255)
                                                       acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  >  2:678557/1‑93 (MQ=255)
                                                       acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  >  1:189357/1‑93 (MQ=255)
                                                        caTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAt                                 >  2:6277601/1‑93 (MQ=255)
                                                        caTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAt                                 <  2:5162987/93‑1 (MQ=255)
                                                         atatTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATc                                >  1:4748432/1‑93 (MQ=255)
                                                          tatTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATcc                               <  2:3371130/93‑1 (MQ=255)
                                                          tatTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATcc                               <  2:4725407/93‑1 (MQ=255)
                                                            ttCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCgg                             >  2:2092089/1‑93 (MQ=255)
                                                               aaaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATg                          >  1:705568/1‑93 (MQ=255)
                                                                 aGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGa                        >  1:3934882/1‑93 (MQ=255)
                                                                 aGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGa                        <  2:3080531/93‑1 (MQ=255)
                                                                  gCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                       <  1:1064446/93‑1 (MQ=255)
                                                                   ccGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAg                      <  2:1525045/93‑1 (MQ=255)
                                                                   ccGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAg                      <  2:1245261/93‑1 (MQ=255)
                                                                    cGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGt                     <  2:6303375/93‑1 (MQ=255)
                                                                    cGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGt                     <  1:2634750/93‑1 (MQ=255)
                                                                     gTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    <  1:2261577/93‑1 (MQ=255)
                                                                      ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  1:2520267/1‑93 (MQ=255)
                                                                      ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  1:4722812/1‑93 (MQ=255)
                                                                       tgaGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGa                  >  1:5278293/3‑93 (MQ=255)
                                                                       tAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGa                  >  1:4601523/1‑93 (MQ=255)
                                                                       tAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGa                  >  2:4029175/1‑93 (MQ=255)
                                                                         aGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                <  2:2589237/93‑1 (MQ=255)
                                                                            aTCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  2:1170520/93‑1 (MQ=255)
                                                                             tCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAg            <  2:2410197/93‑1 (MQ=255)
                                                                              cagcagCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc           <  1:3121413/93‑1 (MQ=255)
                                                                                gcagcGGCATGTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgc         <  2:320073/93‑1 (MQ=255)
                                                                                  agcGGCAT‑TTCCTGCGGATCGAGGTCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTg       >  1:764201/1‑93 (MQ=255)
                                                                                   gcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt      >  2:501807/1‑93 (MQ=255)
                                                                                     ggCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGttt    >  1:3527396/1‑93 (MQ=255)
                                                                                     ggCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGttt    <  1:5691762/93‑1 (MQ=255)
                                                                                      gCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTc   >  2:4321586/1‑93 (MQ=255)
                                                                                      gCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTc   >  2:1912688/1‑93 (MQ=255)
                                                                                      gCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTc   >  2:1066680/1‑93 (MQ=255)
                                                                                             |                                                                                       
TTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115024‑3115202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  NC_000913/3115026‑3115209
                                                                                             |                                                                                             
TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑Cctg                                                                                            <  SRR4302547.5499637/94‑4 (MQ=60)
 GCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGC                                                                                            <  SRR4302547.4255227/94‑1 (MQ=60)
  GTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCG                                                                                           <  SRR4302547.5186325/94‑1 (MQ=60)
  CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCG                                                                                           <  SRR4302547.4036562/94‑1 (MQ=60)
   TGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGG                                                                                          >  SRR4302547.6460631/1‑94 (MQ=60)
   TGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGG                                                                                          >  SRR4302547.1893049/1‑94 (MQ=60)
    GGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGA                                                                                         >  SRR4302547.5711813/1‑94 (MQ=60)
     GTATCGGCGCGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGAT                                                                                        <  SRR4302547.5161144/94‑1 (MQ=60)
      TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATC                                                                                       >  SRR4302547.1097242/1‑94 (MQ=60)
      TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATC                                                                                       <  SRR4302547.3816502/94‑1 (MQ=60)
      TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATC                                                                                       <  SRR4302547.5967266/94‑1 (MQ=60)
       ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCG                                                                                      <  SRR4302547.5076438/94‑1 (MQ=60)
       ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCG                                                                                      <  SRR4302547.5953377/94‑1 (MQ=60)
       ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCG                                                                                      <  SRR4302547.1737344/94‑1 (MQ=60)
       ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCG                                                                                      >  SRR4302547.3298737/1‑94 (MQ=60)
        TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGA                                                                                     <  SRR4302547.5244494/94‑1 (MQ=60)
        TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGA                                                                                     <  SRR4302547.6201142/94‑1 (MQ=60)
        TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGA                                                                                     <  SRR4302547.918128/94‑1 (MQ=60)
        TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGA                                                                                     <  SRR4302547.164462/94‑1 (MQ=60)
         CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAG                                                                                    >  SRR4302547.5474367/1‑94 (MQ=60)
         CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAG                                                                                    <  SRR4302547.2506616/94‑1 (MQ=60)
         CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAG                                                                                    >  SRR4302547.2629935/1‑94 (MQ=60)
         CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAG                                                                                    <  SRR4302547.1553907/94‑1 (MQ=60)
         CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGCCACATATTCAAAGCCGTTAAGGATCACCAGCGGCA‑T‑TTCCTGCGGATCGCG                                                                                    >  SRR4302547.6486254/1‑94 (MQ=60)
          TGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGA                                                                                   >  SRR4302547.786857/1‑94 (MQ=60)
            CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATC                                                                                 <  SRR4302547.868745/94‑1 (MQ=60)
            CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATC                                                                                 <  SRR4302547.1257690/94‑1 (MQ=60)
            CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATC                                                                                 <  SRR4302547.771171/94‑1 (MQ=60)
            CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATC                                                                                 <  SRR4302547.6421918/94‑1 (MQ=60)
            CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATC                                                                                 >  SRR4302547.4311692/1‑94 (MQ=60)
              CGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGC                                                                               >  SRR4302547.5888163/1‑94 (MQ=60)
              CGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGC                                                                               <  SRR4302547.5235423/94‑1 (MQ=60)
               GCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCC                                                                              <  SRR4302547.5981755/94‑1 (MQ=60)
                CAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCA                                                                             >  SRR4302547.1932595/1‑94 (MQ=60)
                 AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCAT                                                                            >  SRR4302547.2863746/1‑94 (MQ=60)
                 AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCAT                                                                            >  SRR4302547.2418752/1‑94 (MQ=60)
                 AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCAT                                                                            <  SRR4302547.5415393/94‑1 (MQ=60)
                 AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCAT                                                                            >  SRR4302547.3634218/1‑94 (MQ=60)
                  GCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATA                                                                           <  SRR4302547.1436311/94‑1 (MQ=60)
                        TTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACT                                                                     >  SRR4302547.4480556/1‑94 (MQ=60)
                        TTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACT                                                                     <  SRR4302547.1729925/94‑1 (MQ=60)
                        TTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACT                                                                     <  SRR4302547.5458065/94‑1 (MQ=60)
                         TGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TT‑CTGCGGATCGAGACCGCCATAGCTACTT                                                                    >  SRR4302547.1167437/1‑94 (MQ=60)
                           CATAAGGATCGTTACCCACCTGAGTCACATATTCAAATCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCAGAGCTACTTAA                                                                  >  SRR4302547.2318301/1‑94 (MQ=60)
                           CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAA                                                                  <  SRR4302547.1212792/94‑1 (MQ=60)
                           CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAA                                                                  >  SRR4302547.5458632/1‑94 (MQ=60)
                            ataaggcgcctccccccCCTGAGTCAAATATTCAAAGCCTTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 <  SRR4302547.213236/77‑1 (MQ=60)
                            ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302547.5011381/1‑94 (MQ=60)
                            ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302547.1979562/1‑94 (MQ=60)
                            ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302547.5478469/1‑94 (MQ=60)
                            ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 <  SRR4302547.5625208/94‑1 (MQ=60)
                            ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302547.3335433/1‑94 (MQ=60)
                              AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATG                                                               >  SRR4302547.233739/1‑94 (MQ=60)
                               AGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGC                                                              >  SRR4302547.4308932/1‑94 (MQ=60)
                                GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                             <  SRR4302547.6690607/94‑1 (MQ=60)
                                  ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           <  SRR4302547.3904136/94‑1 (MQ=60)
                                  ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           <  SRR4302547.3382770/94‑1 (MQ=60)
                                   TCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAA                                                          >  SRR4302547.5733618/1‑94 (MQ=60)
                                   TCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAA                                                          >  SRR4302547.4531660/1‑94 (MQ=60)
                                    CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                         <  SRR4302547.5528880/94‑1 (MQ=60)
                                    CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                         <  SRR4302547.2892056/94‑1 (MQ=60)
                                     GTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGTCGTAGCTACTTAAATGCTCAACA                                                        >  SRR4302547.2689442/1‑94 (MQ=60)
                                     GTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACA                                                        >  SRR4302547.3876874/1‑94 (MQ=60)
                                      TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                       >  SRR4302547.6272892/1‑94 (MQ=60)
                                      TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                       <  SRR4302547.6784574/94‑1 (MQ=60)
                                      TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                       >  SRR4302547.5402966/1‑94 (MQ=60)
                                       TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      <  SRR4302547.5427787/94‑1 (MQ=60)
                                       TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      >  SRR4302547.1463238/1‑94 (MQ=60)
                                       TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      <  SRR4302547.1126366/94‑1 (MQ=60)
                                       TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      >  SRR4302547.4267528/1‑94 (MQ=60)
                                        ACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAG                                                     <  SRR4302547.727272/94‑1 (MQ=60)
                                        ACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAG                                                     <  SRR4302547.6062780/94‑1 (MQ=60)
                                         CCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGA                                                    <  SRR4302547.6587968/94‑1 (MQ=60)
                                         CCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGA                                                    >  SRR4302547.4872515/1‑94 (MQ=60)
                                         CCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGA                                                    >  SRR4302547.2382571/1‑94 (MQ=60)
                                         CCCACCTGAGTCACATATTCAAAGCCGGTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGCGGTCGCCATAGCTACTTAAATGCTCAACAGAGA                                                    >  SRR4302547.5822170/1‑94 (MQ=60)
                                          CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   >  SRR4302547.2287797/1‑94 (MQ=60)
                                          CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302547.5815192/94‑1 (MQ=60)
                                          CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302547.3190072/94‑1 (MQ=60)
                                           CACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATG                                                  >  SRR4302547.3890189/1‑94 (MQ=60)
                                            CCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGC                                                 <  SRR4302547.6086889/94‑1 (MQ=60)
                                            ACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGC                                                 <  SRR4302547.6308379/94‑1 (MQ=60)
                                            ACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGC                                                 >  SRR4302547.5965949/1‑94 (MQ=60)
                                            ACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGC                                                 <  SRR4302547.6104139/94‑1 (MQ=60)
                                             CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                >  SRR4302547.4584432/1‑94 (MQ=60)
                                             CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                >  SRR4302547.4823229/1‑94 (MQ=60)
                                                GAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGC                                             >  SRR4302547.4345520/1‑94 (MQ=60)
                                                  GTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAA                                           <  SRR4302547.3701096/94‑1 (MQ=60)
                                                   TCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                          <  SRR4302547.505736/94‑1 (MQ=60)
                                                   TCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                          <  SRR4302547.5554794/94‑1 (MQ=60)
                                                    CACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAA                                         >  SRR4302547.192921/1‑94 (MQ=60)
                                                     ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        >  SRR4302547.5073632/1‑94 (MQ=60)
                                                     ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        <  SRR4302547.3008659/94‑1 (MQ=60)
                                                      CATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTTCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATC                                       >  SRR4302547.3433865/1‑94 (MQ=60)
                                                      CATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATC                                       >  SRR4302547.4846828/1‑94 (MQ=60)
                                                        TATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                     >  SRR4302547.853337/1‑94 (MQ=60)
                                                         ATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGG                                    <  SRR4302547.1785799/94‑1 (MQ=60)
                                                          TTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGA                                   <  SRR4302547.3144628/94‑1 (MQ=60)
                                                          TTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGA                                   <  SRR4302547.3581148/94‑1 (MQ=60)
                                                           TCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAT                                  <  SRR4302547.4072835/94‑1 (MQ=60)
                                                            CAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATG                                 >  SRR4302547.718603/1‑94 (MQ=60)
                                                              AAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGA                               >  SRR4302547.2655802/1‑94 (MQ=60)
                                                              AAGCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGA                               >  SRR4302547.4014901/1‑94 (MQ=60)
                                                                GCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             <  SRR4302547.5311389/94‑1 (MQ=60)
                                                                GCCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             <  SRR4302547.1085630/94‑1 (MQ=60)
                                                                 CCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                            >  SRR4302547.2333814/1‑94 (MQ=60)
                                                                 CCGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                            <  SRR4302547.2993230/94‑1 (MQ=60)
                                                                  CGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCGACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           >  SRR4302547.148727/1‑94 (MQ=60)
                                                                  CGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           <  SRR4302547.2687813/94‑1 (MQ=60)
                                                                  CGTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           <  SRR4302547.2985701/94‑1 (MQ=60)
                                                                   GTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302547.2266824/1‑94 (MQ=60)
                                                                   GTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          <  SRR4302547.2307270/94‑1 (MQ=60)
                                                                   GTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302547.2571208/1‑94 (MQ=60)
                                                                   GTTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302547.4820603/1‑94 (MQ=60)
                                                                    TTGAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGA                         >  SRR4302547.5387157/1‑94 (MQ=60)
                                                                    TTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGA                         >  SRR4302547.4696754/1‑94 (MQ=60)
                                                                    TTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGA                         >  SRR4302547.4781217/1‑94 (MQ=60)
                                                                    TTAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGA                         >  SRR4302547.5765925/1‑94 (MQ=60)
                                                                     TAAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAA                        >  SRR4302547.688839/1‑94 (MQ=60)
                                                                      AAGGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                       <  SRR4302547.2881788/94‑1 (MQ=60)
                                                                        GGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGC                     <  SRR4302547.3337690/94‑1 (MQ=60)
                                                                        GGATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGC                     <  SRR4302547.5528070/94‑1 (MQ=60)
                                                                         GATCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCA                    <  SRR4302547.4807047/94‑1 (MQ=60)
                                                                           TCAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGC                  <  SRR4302547.6275991/94‑1 (MQ=60)
                                                                            CAGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCG                 <  SRR4302547.3183309/94‑1 (MQ=60)
                                                                             AGCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGC                >  SRR4302547.2528843/1‑94 (MQ=60)
                                                                              GCAGCGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCT               <  SRR4302547.1373301/94‑1 (MQ=60)
                                                                               CAGCGGCA‑TGTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTG              <  SRR4302547.2808923/94‑1 (MQ=60)
                                                                               CAGCGGCA‑T‑TTCCTGCGGATCGAGGTCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTG              >  SRR4302547.778606/1‑94 (MQ=60)
                                                                                  CGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTT           >  SRR4302547.3643531/1‑94 (MQ=60)
                                                                                  CGGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTT           >  SRR4302547.3599419/1‑94 (MQ=60)
                                                                                   GGCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTC          <  SRR4302547.5809315/94‑1 (MQ=60)
                                                                                    GCA‑T‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC         >  SRR4302547.3140121/1‑94 (MQ=60)
                                                                                     cgcatttcCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTC          <  SRR4302547.5074048/86‑1 (MQ=60)
                                                                                        T‑TC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTA     <  SRR4302547.344682/94‑1 (MQ=60)
                                                                                             CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  <  SRR4302547.589460/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑T‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  NC_000913/3115026‑3115209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑