Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,130,334 A→G V30V (GTT→GTC)  b2983 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,130,3340AG100.0% 629.6 / NA 175V30V (GTT→GTC) b2983hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (71/104);  total (71/104)

Only 100 of 208 total aligned reads displayed.

CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA  >  NC_000913/3130244‑3130423
                                                                                           |                                                                                         
cGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCacgac                                                                                         <  2:3816138/93‑1 (MQ=255)
 gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCCGCAAGGCGCTCacgaca                                                                                        >  1:176922/1‑93 (MQ=255)
 gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCacgaca                                                                                        <  2:2034880/93‑1 (MQ=255)
 gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCacgaca                                                                                        <  1:5750340/93‑1 (MQ=255)
 gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCacgaca                                                                                        <  1:6044950/93‑1 (MQ=255)
 gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGGCATCCCTTTA‑CCCGCCCACCAGAGCGCCAACAGACCCAGCAAGGCGCTCacgaca                                                                                        >  2:4569011/1‑93 (MQ=255)
  aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCCCGACAt                                                                                       >  2:1355377/1‑93 (MQ=255)
  aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAt                                                                                       >  2:3621811/1‑93 (MQ=255)
  aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAt                                                                                       >  1:24885/1‑93 (MQ=255)
   ttGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAtt                                                                                      <  1:3081828/93‑1 (MQ=255)
    tGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTa                                                                                     >  2:3953599/1‑93 (MQ=255)
     gCACAATCACCAGTACGCCAAACATGGCTGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTAc                                                                                    >  2:5641003/1‑93 (MQ=255)
     gCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTAc                                                                                    >  2:5851762/1‑93 (MQ=255)
       acaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTg                                                                                  <  2:4769910/93‑1 (MQ=255)
       acaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACCTTACTg                                                                                  >  1:865504/1‑93 (MQ=255)
         aaTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAAGCGCTCACGACATTACTAGa                                                                                >  2:5407062/1‑93 (MQ=255)
              ccAGTACGCAAAACATGGCGGGCGTCATCCCTTTC‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTa                                                                           <  2:5544586/93‑1 (MQ=255)
                 gtccGCCAAACATGGCGGGCGTCATCCCTTCC‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAcc                                                                        <  1:2874322/90‑1 (MQ=255)
                 gTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAcc                                                                        <  1:997652/93‑1 (MQ=255)
                 gTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAcc                                                                        >  1:67827/1‑93 (MQ=255)
                  taggCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGGAAGGCGCTCACGACATTACTGGAGCCTAACCa                                                                       <  1:5332336/90‑1 (MQ=255)
                  tACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCa                                                                       <  1:4235645/93‑1 (MQ=255)
                  tACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGACTAACCa                                                                       >  1:5874677/1‑93 (MQ=255)
                   aCGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAg                                                                      <  1:2298613/93‑1 (MQ=255)
                   aCGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAg                                                                      <  2:1143047/93‑1 (MQ=255)
                     gCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgc                                                                    >  1:2278653/1‑93 (MQ=255)
                     gCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgc                                                                    >  2:4273195/1‑93 (MQ=255)
                      ccAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCCgggc                                                                   >  2:3591670/1‑91 (MQ=255)
                      ccAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcg                                                                   <  2:2950896/93‑1 (MQ=255)
                        aaaCATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCt                                                                 >  1:3014820/1‑93 (MQ=255)
                        aaaCATGGCGGGCGTCATCCCTTTA‑CCCGCACCCGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCt                                                                 >  1:463790/1‑93 (MQ=255)
                          aCATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                                               <  2:842009/93‑1 (MQ=255)
                           cATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAtt                                                              <  1:5052818/93‑1 (MQ=255)
                            aTGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTg                                                             <  2:5097816/93‑1 (MQ=255)
                            aTGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTg                                                             <  2:6012552/93‑1 (MQ=255)
                              ggggggcgTCA‑CCCTTTACCCGCCACACGAGACCAACAACACCCCCAAGAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCg                                                           <  1:5469694/90‑1 (MQ=255)
                              ggcgggcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCg                                                           >  2:5110449/1‑93 (MQ=255)
                               gcgggcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGa                                                          <  1:2035605/93‑1 (MQ=255)
                                 gggcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaat                                                        >  1:35367/1‑93 (MQ=255)
                                  ggcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGCCCAAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaata                                                       <  2:5548017/93‑1 (MQ=255)
                                  ggcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaata                                                       <  2:4003183/93‑1 (MQ=255)
                                  ggcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaata                                                       >  2:2474082/1‑93 (MQ=255)
                                   gcgTCATCCCTTTC‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                      <  2:2666146/93‑1 (MQ=255)
                                    cgTCATCCCTTTC‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  1:1604293/93‑1 (MQ=255)
                                    cgTCAGCCCTTTA‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  1:5975936/93‑1 (MQ=255)
                                     gTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                    <  1:3055324/93‑1 (MQ=255)
                                     gTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                    >  2:5222277/1‑93 (MQ=255)
                                      tCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                   >  1:1557225/1‑93 (MQ=255)
                                      tCACCCCTTTC‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAACAATgg                                                   <  1:4104609/93‑1 (MQ=255)
                                       cATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  <  1:2167010/93‑1 (MQ=255)
                                        aTCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                                 <  1:6136099/93‑1 (MQ=255)
                                        aTCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                                 <  2:1924963/93‑1 (MQ=255)
                                         tCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                >  2:3329107/1‑93 (MQ=255)
                                         tCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                <  2:2969003/93‑1 (MQ=255)
                                           ccTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGGGa                                              >  1:4872001/1‑93 (MQ=255)
                                           ccTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGa                                              >  1:1456714/1‑93 (MQ=255)
                                             tttA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            >  1:3464127/1‑93 (MQ=255)
                                              ttccccGGCACAGGAGAGGCCAAACACACCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            <  1:1669238/89‑1 (MQ=255)
                                              ttA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           >  1:334389/1‑93 (MQ=255)
                                               tA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                                          >  2:788551/1‑93 (MQ=255)
                                               tA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                                          >  1:3745485/1‑93 (MQ=255)
                                                    ggCACACGAGAGCGCCAACAGCCCCAGCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                                      <  2:2704711/93‑1 (MQ=255)
                                                    ggCACACGAGACCGCAAACACCCCCAGAAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                                      <  2:4728588/93‑1 (MQ=255)
                                                     gCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCa                                     <  2:2125896/93‑1 (MQ=255)
                                                     gCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCa                                     <  1:712669/93‑1 (MQ=255)
                                                       ccacGAGACCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                   <  1:4910709/92‑1 (MQ=255)
                                                        cacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                                  >  1:5081333/1‑93 (MQ=255)
                                                        cacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                                  <  1:6008184/93‑1 (MQ=255)
                                                        cacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                                  <  2:1821152/93‑1 (MQ=255)
                                                           gagaGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCa                               <  2:2762555/93‑1 (MQ=255)
                                                           gagaCCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGAGAATAATGGTGCGAAACGCGCCATCTGCa                               <  1:4759307/93‑1 (MQ=255)
                                                             gagCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaac                             >  2:4283236/1‑92 (MQ=255)
                                                               gcgcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAcc                           <  2:1508310/93‑1 (MQ=255)
                                                                  ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                        >  2:5825580/1‑93 (MQ=255)
                                                                  ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                        >  2:5379067/1‑93 (MQ=255)
                                                                   cAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATg                       <  2:2013268/93‑1 (MQ=255)
                                                                      cAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    >  2:4130345/1‑93 (MQ=255)
                                                                      cAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    <  2:2928670/93‑1 (MQ=255)
                                                                      aaGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    <  2:1492126/92‑1 (MQ=255)
                                                                        gtcccAGCAAGGCTCGCCCGACATGACTGGAGCCTAACCAGGCGCAATTGCGAATAATGGTGCGAAACGCGCCAGCTGCAAACCAATGTTTGa                  <  1:5951768/91‑1 (MQ=255)
                                                                        aaCCCAGAAGGGCTCACACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  <  1:26364/92‑1 (MQ=255)
                                                                         aCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAt                 >  1:3822273/1‑93 (MQ=255)
                                                                         aCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAt                 <  2:5081947/93‑1 (MQ=255)
                                                                          cccGGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATg                <  1:2764153/93‑1 (MQ=255)
                                                                           ccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGt               <  2:3247190/93‑1 (MQ=255)
                                                                           ccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGt               >  2:3128669/1‑93 (MQ=255)
                                                                           caaGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGt               <  2:4902599/91‑1 (MQ=255)
                                                                            cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGtt              <  1:103639/93‑1 (MQ=255)
                                                                            cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGtt              >  2:691445/1‑93 (MQ=255)
                                                                            cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGtt              >  1:4662284/1‑93 (MQ=255)
                                                                               aaaGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           <  2:217497/92‑1 (MQ=255)
                                                                                 aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         >  1:3090373/1‑93 (MQ=255)
                                                                                 aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         <  1:1951262/93‑1 (MQ=255)
                                                                                    cgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCg      <  2:1468435/93‑1 (MQ=255)
                                                                                     gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGc     <  1:3683313/93‑1 (MQ=255)
                                                                                     gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGc     >  2:4382745/1‑93 (MQ=255)
                                                                                     gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATATTAAAACCCGc     <  2:2499870/93‑1 (MQ=255)
                                                                                      cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGcc    <  2:4630239/93‑1 (MQ=255)
                                                                                       tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa   >  1:3369072/1‑93 (MQ=255)
                                                                                        cacgACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAATCCGCCaa  <  2:5893933/93‑1 (MQ=255)
                                                                                           |                                                                                         
CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA  >  NC_000913/3130244‑3130423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427
                                                                                             |                                                                                             
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCCTCCCTTTACCGGCACCCGAGAGCGCCAACAGACCCCGCAAGGCGATCACC                                                                                               >  SRR4302532.1955829/1‑94 (MQ=60)
 TACGATTGCACAATCCCCAGTACGCAAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGA                                                                                              <  SRR4302532.2793121/94‑1 (MQ=60)
  ACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGAC                                                                                             <  SRR4302532.4146663/94‑1 (MQ=60)
  ACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAAAAGACCCAGCAAGGGGCTCACGAC                                                                                             >  SRR4302532.2365835/1‑94 (MQ=60)
  ACGATTGCACAACACCCGGTACGCAAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACGGACCCAGCAAGGCGCTCACGAC                                                                                             <  SRR4302532.2746417/94‑1 (MQ=60)
   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCCGCAAGGCGCTCACGACA                                                                                            >  SRR4302532.182850/1‑94 (MQ=60)
   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACA                                                                                            >  SRR4302532.2064087/1‑94 (MQ=60)
    GATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAT                                                                                           <  SRR4302532.5950500/94‑1 (MQ=60)
    GATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAT                                                                                           <  SRR4302532.6253671/94‑1 (MQ=60)
    GATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAT                                                                                           >  SRR4302532.25881/1‑94 (MQ=60)
     ATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATT                                                                                          <  SRR4302532.6314473/94‑1 (MQ=60)
      TTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTA                                                                                         <  SRR4302532.3183751/94‑1 (MQ=60)
         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTCCCGGCCCACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTG                                                                                      <  SRR4302532.346183/94‑1 (MQ=60)
         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACCTTACTG                                                                                      >  SRR4302532.895181/1‑94 (MQ=60)
         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTG                                                                                      <  SRR4302532.4264201/94‑1 (MQ=60)
               CACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGGGCCAACAGACCCAGCAAAGCGGCCACGACATTAATGGAGCCT                                                                                >  SRR4302532.2757843/1‑94 (MQ=60)
                cccagcacaCCAAACAGGGCGGACGCCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGAAAGCCGCTCACGACATTACTGGAGCCTA                                                                               <  SRR4302532.2784074/85‑1 (MQ=60)
                 CCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAA                                                                              >  SRR4302532.5042731/1‑94 (MQ=60)
                 CCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAA                                                                              >  SRR4302532.3148875/1‑94 (MQ=60)
                  CAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAC                                                                             >  SRR4302532.3190505/1‑94 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            >  SRR4302532.70264/1‑94 (MQ=60)
                    GTCCGCCAAACATGGCGGGCGTCATCCCTTCCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302532.2970429/94‑1 (MQ=60)
                    GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302532.1031653/94‑1 (MQ=60)
                    GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302532.6148191/94‑1 (MQ=60)
                    GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGACTAACCA                                                                           >  SRR4302532.6078222/1‑94 (MQ=60)
                     gacgcaaaacggGGCGGGCGTCACCCCTTCCCCGCCACACGAGAGCGCAAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAG                                                                          <  SRR4302532.2787331/82‑1 (MQ=43)
                     TAGGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGGAAGGCGCTCACGACATTACTGGAGCCTAACCAG                                                                          <  SRR4302532.5517247/94‑1 (MQ=60)
                     TACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAG                                                                          <  SRR4302532.4377884/94‑1 (MQ=60)
                      ACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCCCACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGG                                                                         <  SRR4302532.3488478/94‑1 (MQ=60)
                      ACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGG                                                                         <  SRR4302532.2374980/94‑1 (MQ=60)
                      ACGCCAAACATGGCGGGCGGCATCCCTTTAACGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCCAAACAGG                                                                         >  SRR4302532.4535443/1‑94 (MQ=60)
                       cccaaaacggGGCGGGCGTCCCCCCTTTCCCGGCACGCGAGAGCGCAAACAGCCCCAGCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGC                                                                        <  SRR4302532.1786991/84‑1 (MQ=35)
                       CGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGC                                                                        >  SRR4302532.2354279/1‑94 (MQ=60)
                        GCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCG                                                                       >  SRR4302532.1862052/1‑94 (MQ=60)
                         CCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGC                                                                      >  SRR4302532.4825292/1‑94 (MQ=60)
                          CAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCT                                                                     >  SRR4302532.4543915/1‑94 (MQ=60)
                          CAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCT                                                                     >  SRR4302532.3114315/1‑94 (MQ=60)
                          CAAACATGGCGGGCGTCATCCCTTTACCCGCACCCGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCT                                                                     >  SRR4302532.479937/1‑94 (MQ=60)
                             ACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACGTTACTGGAGCCTAACCAGGCGCTATT                                                                  >  SRR4302532.3157279/1‑94 (MQ=60)
                              catctcggtcgtcctcccttagcaGGCACACGAGAGCCCCAACAGACCCAGCAAGGCGCTCACGATATTACTGGAGCCTAACCAGGCGCTATTT                                                                 <  SRR4302532.5535472/70‑1 (MQ=60)
                              CATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTG                                                                 <  SRR4302532.5226219/94‑1 (MQ=60)
                                gggcggccttcaccctttccccgccaccggagaccgcaaccagccccagaagggccCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCG                                                               <  SRR4302532.2597592/38‑1 (MQ=60)
                                 ggggggcgtcaccctttacccgccacacgagaccaacaacacccccaagAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGA                                                              <  SRR4302532.5659320/45‑1 (MQ=60)
                                  GCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAA                                                             >  SRR4302532.698610/1‑94 (MQ=60)
                                  GCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAA                                                             <  SRR4302532.2103416/94‑1 (MQ=60)
                                   CGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAAT                                                            >  SRR4302532.36667/1‑94 (MQ=60)
                                    GGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATA                                                           <  SRR4302532.3989104/94‑1 (MQ=60)
                                      GCGTCACCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT                                                         <  SRR4302532.3860849/94‑1 (MQ=60)
                                       CGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302532.1658603/94‑1 (MQ=60)
                                       CGTCAGCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302532.6182491/94‑1 (MQ=60)
                                        GTCATCCTTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302532.3926389/94‑1 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302532.3156203/94‑1 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       >  SRR4302532.1609874/1‑94 (MQ=60)
                                         TCACCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAACAATGGT                                                      <  SRR4302532.4242367/94‑1 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302532.2239491/94‑1 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302532.706389/94‑1 (MQ=60)
                                           ATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGC                                                    <  SRR4302532.6347779/94‑1 (MQ=60)
                                            TCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   <  SRR4302532.543338/94‑1 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGGGA                                                  >  SRR4302532.5039493/1‑94 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302532.1505829/1‑94 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302532.3935251/1‑94 (MQ=60)
                                               CTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                >  SRR4302532.3580155/1‑94 (MQ=60)
                                                ttccccggcacaggagaggccaaacacACCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302532.1725722/67‑1 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302532.6196494/94‑1 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302532.2110739/94‑1 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               >  SRR4302532.345973/1‑94 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302532.2258954/94‑1 (MQ=60)
                                                 TTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACG                                              >  SRR4302532.3871374/1‑94 (MQ=60)
                                                     CGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCC                                          >  SRR4302532.36191/1‑94 (MQ=60)
                                                       GCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAT                                        <  SRR4302532.737014/94‑1 (MQ=60)
                                                         CCACGAGACCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      <  SRR4302532.5079443/94‑1 (MQ=60)
                                                         ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      >  SRR4302532.5256047/1‑94 (MQ=60)
                                                          CACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302532.6215659/94‑1 (MQ=60)
                                                          CACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     >  SRR4302532.1139319/1‑94 (MQ=60)
                                                             GAGACCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGAGAATAATGGTGCGAAACGCGCCATCTGCAA                                  <  SRR4302532.4922179/94‑1 (MQ=60)
                                                              AGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAA                                 >  SRR4302532.964/1‑94 (MQ=60)
                                                                  CCCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAA                             <  SRR4302532.6031943/94‑1 (MQ=60)
                                                                   GCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            <  SRR4302532.3351869/94‑1 (MQ=60)
                                                                   GCAAACAGCCCGAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            <  SRR4302532.298610/94‑1 (MQ=60)
                                                                     caccacacaCAGCAGGGCGCGCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          <  SRR4302532.2557299/85‑1 (MQ=60)
                                                                      AACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302532.5955370/94‑1 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         >  SRR4302532.5631088/1‑94 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         >  SRR4302532.2742171/1‑94 (MQ=60)
                                                                        aagcCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       <  SRR4302532.3739432/90‑1 (MQ=60)
                                                                         AGCCCAAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      <  SRR4302532.2594357/94‑1 (MQ=60)
                                                                          GACCCCGCAAGCGGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGGCACCTGCAAACCAATGTTTGAT                     <  SRR4302532.5243216/94‑1 (MQ=60)
                                                                          GACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     >  SRR4302532.3951315/1‑94 (MQ=60)
                                                                           ACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATG                    <  SRR4302532.1767499/94‑1 (MQ=60)
                                                                           ACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATG                    <  SRR4302532.886918/94‑1 (MQ=60)
                                                                            CCCGGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   <  SRR4302532.2856964/94‑1 (MQ=60)
                                                                            CCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   <  SRR4302532.6175223/94‑1 (MQ=60)
                                                                             CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  >  SRR4302532.4821246/1‑94 (MQ=60)
                                                                              CAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 <  SRR4302532.107221/94‑1 (MQ=60)
                                                                              CAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 >  SRR4302532.1700350/1‑94 (MQ=60)
                                                                               AGCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                <  SRR4302532.5755647/94‑1 (MQ=60)
                                                                                GCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAA               <  SRR4302532.4805117/94‑1 (MQ=60)
                                                                                 CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              >  SRR4302532.1559118/1‑94 (MQ=60)
                                                                                 AAAGGCCCTCACGACATTACTGGACCCTAACCAGGCCCTATGGCGAATAAGGGTGCAAAACGCGCCACCTGCAAACCAATGTTTGATGTTAAAA              <  SRR4302532.1542622/94‑1 (MQ=60)
                                                                                  AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             >  SRR4302532.6244573/1‑94 (MQ=60)
                                                                                  AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             >  SRR4302532.3192770/1‑94 (MQ=60)
                                                                                  AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             <  SRR4302532.2871472/94‑1 (MQ=60)
                                                                                  AAGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             <  SRR4302532.1620212/94‑1 (MQ=60)
                                                                                   AGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACC            <  SRR4302532.2016115/94‑1 (MQ=60)
                                                                                   AGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACC            <  SRR4302532.2534180/94‑1 (MQ=60)
                                                                                       GCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCC        <  SRR4302532.3807021/94‑1 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       >  SRR4302532.3481703/1‑94 (MQ=60)
                                                                                         TCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA      <  SRR4302532.5107227/94‑1 (MQ=60)
                                                                                          CACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAT     >  SRR4302532.6117193/1‑94 (MQ=60)
                                                                                          CACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAT     <  SRR4302532.4925321/94‑1 (MQ=60)
                                                                                           ACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATT    <  SRR4302532.685987/94‑1 (MQ=60)
                                                                                           ACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATT    <  SRR4302532.685989/94‑1 (MQ=60)
                                                                                            CGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTC   >  SRR4302532.3651794/1‑94 (MQ=60)
                                                                                            CGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTC   >  SRR4302532.4794932/1‑94 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCTCCAATTCA  <  SRR4302532.6240453/94‑1 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  SRR4302532.2371971/1‑94 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302532.3683656/94‑1 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302532.1391415/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑