Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,138,885	T→C	E139E (GAA→GAG)	hybD ←	putative processing element for hydrogenase‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,138,885	0	T	C	87.2%	752.1 / 81.0	274	E139E (GAA→GAG)	hybD	putative processing element for hydrogenase‑2
Reads supporting (aligned to +/- strand): ref base T (18/17); new base C (127/112); total (145/129)
Fisher's exact test for biased strand distribution p-value = 8.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

CCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTC  >  NC_000913/3138795‑3138977

ccTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTc                                                                                            >  1:741098/1‑93 (MQ=255)

 cTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCa                                                                                           <  2:1343116/93‑1 (MQ=255)

 cTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCa                                                                                           <  1:3463180/93‑1 (MQ=255)

 cTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCa                                                                                           >  2:5259827/1‑93 (MQ=255)

  tGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCaa                                                                                          <  2:632669/93‑1 (MQ=255)

  tGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCaa                                                                                          >  2:3824413/1‑93 (MQ=255)

  tGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCaa                                                                                          <  1:1901785/93‑1 (MQ=255)

   gCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAAt                                                                                         <  2:4131984/93‑1 (MQ=255)

   gCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAAt                                                                                         >  2:4643879/1‑93 (MQ=255)

    cTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATc                                                                                        <  1:1000801/93‑1 (MQ=255)

     tATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCa                                                                                       <  1:431424/93‑1 (MQ=255)

      aTCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCAt                                                                                      <  2:1049136/93‑1 (MQ=255)

      aTCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCAt                                                                                      <  1:1272656/93‑1 (MQ=255)

       tctcTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCAtt                                                                                     >  1:748635/1‑93 (MQ=255)

        ctctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTg                                                                                    >  2:6428415/1‑93 (MQ=255)

          ctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCt                                                                                  <  2:210057/93‑1 (MQ=255)

            tCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTc                                                                                >  1:1331380/1‑93 (MQ=255)

             cAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCa                                                                               >  2:5744312/1‑93 (MQ=255)

              aGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCaa                                                                              >  1:3348828/1‑93 (MQ=255)

               gTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAAc                                                                             >  2:2508901/1‑93 (MQ=255)

                tCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAAcc                                                                            <  2:3362279/93‑1 (MQ=255)

                 cATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCg                                                                           >  2:1680597/1‑93 (MQ=255)

                 cATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCg                                                                           <  2:6107605/93‑1 (MQ=255)

                  aTGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGt                                                                          >  2:3971670/1‑93 (MQ=255)

                   tGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTc                                                                         <  2:586719/93‑1 (MQ=255)

                    gAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCACAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCGACCGTCg                                                                        >  2:3186322/1‑93 (MQ=255)

                      aTCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGc                                                                      >  2:461636/1‑93 (MQ=255)

                       tCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCg                                                                     >  1:5608845/1‑93 (MQ=255)

                        cGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGt                                                                    <  1:5783619/93‑1 (MQ=255)

                         gCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGtt                                                                   <  1:3099412/93‑1 (MQ=255)

                         gCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGtt                                                                   >  1:4653488/1‑93 (MQ=255)

                          ccTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTa                                                                  <  1:4824617/93‑1 (MQ=255)

                           cTCCCGTGGGATGGCTTCCCCGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTaa                                                                 >  1:6496576/1‑93 (MQ=255)

                           cTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTaa                                                                 >  1:1438452/1‑93 (MQ=255)

                            tCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAg                                                                <  2:374923/93‑1 (MQ=255)

                             cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGc                                                               <  2:3517407/93‑1 (MQ=255)

                              ccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGcc                                                              <  2:848901/93‑1 (MQ=255)

                                gTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGa                                                            >  1:5089175/1‑93 (MQ=255)

                                  gggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtg                                                          >  2:6130913/1‑93 (MQ=255)

                                   ggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgt                                                         <  2:3464013/93‑1 (MQ=255)

                                   ggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgt                                                         >  1:6468883/1‑93 (MQ=255)

                                    gATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgtg                                                        >  1:1570810/1‑93 (MQ=255)

                                     aTGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgtgt                                                       <  1:4164609/93‑1 (MQ=255)

                                      tGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgtgtg                                                      >  1:5569101/1‑93 (MQ=255)

                                       ggCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTgg                                                     >  2:314540/1‑93 (MQ=255)

                                         cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCt                                                   >  2:4967586/1‑93 (MQ=255)

                                         cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCt                                                   >  1:1709466/1‑93 (MQ=255)

                                         cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCt                                                   <  2:2232538/93‑1 (MQ=255)

                                          ttCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTc                                                  <  1:6628574/93‑1 (MQ=255)

                                           tCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTcc                                                 <  2:1494662/93‑1 (MQ=255)

                                           tCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTcc                                                 <  1:1022780/93‑1 (MQ=255)

                                              aCGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGc                                              <  2:302943/93‑1 (MQ=255)

                                                gCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGa                                            <  1:1092847/93‑1 (MQ=255)

                                                gCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGa                                            <  1:4521229/93‑1 (MQ=255)

                                                 ccAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAt                                           <  2:5918628/93‑1 (MQ=255)

                                                  cAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAtt                                          >  2:657253/1‑93 (MQ=255)

                                                   aGATTCACGCAGCGCAGCCAGAACCTGTTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTc                                         <  1:5314522/93‑1 (MQ=255)

                                                   aGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTc                                         >  2:4862156/1‑93 (MQ=255)

                                                    gATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTcc                                        <  2:1462983/93‑1 (MQ=255)

                                                    gATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTcc                                        >  1:2032411/1‑93 (MQ=255)

                                                    gATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTcc                                        <  1:4223619/93‑1 (MQ=255)

                                                    gATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTcc                                        <  1:2224204/93‑1 (MQ=255)

                                                      ttCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCgg                                      <  1:3615263/93‑1 (MQ=255)

                                                        cACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGa                                    >  1:2572129/1‑93 (MQ=255)

                                                         ccgcagcgcagCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGAt                                   <  2:5816916/92‑1 (MQ=255)

                                                         aCGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGAt                                   <  1:3745400/93‑1 (MQ=255)

                                                           gcagcgcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCa                                 >  1:5956287/1‑93 (MQ=255)

                                                           gcagcgcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCa                                 <  1:4372094/93‑1 (MQ=255)

                                                           gcagcgcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCa                                 <  1:897400/93‑1 (MQ=255)

                                                             agcgcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACg                               >  2:77921/1‑93 (MQ=255)

                                                              gcgcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGc                              >  1:5873178/1‑93 (MQ=255)

                                                              gcgcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGc                              <  2:5574579/93‑1 (MQ=255)

                                                               cgcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGcc                             <  2:2290282/93‑1 (MQ=255)

                                                                gcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCg                            <  1:2882511/93‑1 (MQ=255)

                                                                gcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCg                            <  2:4296054/93‑1 (MQ=255)

                                                                gcagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCg                            >  1:2853708/1‑93 (MQ=255)

                                                                 cagcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGa                           <  2:2734716/93‑1 (MQ=255)

                                                                   gcCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAcc                         >  1:3149699/1‑93 (MQ=255)

                                                                   accAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTCAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAcc                         <  2:3557074/92‑1 (MQ=255)

                                                                    ccAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCa                        <  1:2437464/93‑1 (MQ=255)

                                                                     cAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAg                       >  1:3937307/1‑93 (MQ=255)

                                                                      aGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAgg                      >  2:719763/1‑93 (MQ=255)

                                                                       gAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAggg                     >  2:1737410/1‑93 (MQ=255)

                                                                        aaCCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGt                    <  2:6651846/93‑1 (MQ=255)

                                                                        aaCCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGt                    <  1:3240681/93‑1 (MQ=255)

                                                                          ccTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCa                  <  2:3782587/93‑1 (MQ=255)

                                                                          ccTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCa                  <  2:1713894/93‑1 (MQ=255)

                                                                           cTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAg                 <  1:4615344/93‑1 (MQ=255)

                                                                             gCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt               <  1:710029/93‑1 (MQ=255)

                                                                             gCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt               >  1:5987596/1‑93 (MQ=255)

                                                                             gCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt               >  1:630346/1‑93 (MQ=255)

                                                                                 aaGCGCAGTCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCttttt           <  2:946096/93‑1 (MQ=255)

                                                                                  aGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTc          >  2:4411931/1‑93 (MQ=255)

                                                                                  aGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTc          >  1:6057862/1‑93 (MQ=255)

                                                                                  aGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTc          >  1:4756163/1‑93 (MQ=255)

                                                                                     gcAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGa       >  2:4368487/1‑93 (MQ=255)

                                                                                      cAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa      >  2:1245231/1‑93 (MQ=255)

                                                                                       aGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaaa     <  1:969727/93‑1 (MQ=255)

                                                                                        ggTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAAc    <  1:5462963/93‑1 (MQ=255)

                                                                                         gTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACt   <  2:2839534/93‑1 (MQ=255)

CCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTC  >  NC_000913/3138795‑3138977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCG  >  NC_000913/3138793‑3138978

AACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCT                                                                                              >  SRR4302547.3302974/1‑94 (MQ=60)

 ACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTC                                                                                             >  SRR4302547.755009/1‑94 (MQ=60)

   CTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAA                                                                                           <  SRR4302547.3533800/94‑1 (MQ=60)

    TGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAAT                                                                                          <  SRR4302547.1939406/94‑1 (MQ=60)

    TGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAAT                                                                                          >  SRR4302547.2732321/1‑94 (MQ=60)

     GCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATC                                                                                         >  SRR4302547.6447016/1‑94 (MQ=60)

     GCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATC                                                                                         >  SRR4302547.2085309/1‑94 (MQ=60)

      CTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCA                                                                                        <  SRR4302547.3277858/94‑1 (MQ=60)

      CTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCA                                                                                        >  SRR4302547.5691282/1‑94 (MQ=60)

      CTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCA                                                                                        <  SRR4302547.1020311/94‑1 (MQ=60)

       TATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCAT                                                                                       <  SRR4302547.439417/94‑1 (MQ=60)

        ATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATT                                                                                      >  SRR4302547.6590368/1‑94 (MQ=60)

        ATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATT                                                                                      <  SRR4302547.1298278/94‑1 (MQ=60)

        ATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATT                                                                                      >  SRR4302547.762752/1‑94 (MQ=60)

         TCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTG                                                                                     >  SRR4302547.6709515/1‑94 (MQ=60)

         TCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTG                                                                                     <  SRR4302547.1764733/94‑1 (MQ=60)

          CTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGC                                                                                    >  SRR4302547.662473/1‑94 (MQ=60)

          CTCTTCAGGCATGAATCGCCGCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGC                                                                                    <  SRR4302547.6024923/94‑1 (MQ=60)

            CTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTT                                                                                  >  SRR4302547.4720178/1‑94 (MQ=60)

             TTCAGTCATGAATGTCATCCCGTGTGATGTCTTCCACGCCAGATTCACGCAGGGCAGCCAGAACCTTCTCAAGCGCAGGCTCAATCATTGCTTC                                                                                 <  SRR4302547.2096845/94‑1 (MQ=60)

             TTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTC                                                                                 >  SRR4302547.1358141/1‑94 (MQ=60)

              TCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCA                                                                                <  SRR4302547.4798332/94‑1 (MQ=60)

               CAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAA                                                                               >  SRR4302547.3416730/1‑94 (MQ=60)

               CAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAA                                                                               >  SRR4302547.717505/1‑94 (MQ=60)

                AGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAAC                                                                              <  SRR4302547.3211907/94‑1 (MQ=60)

                 GTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACC                                                                             <  SRR4302547.2539929/94‑1 (MQ=60)

                 GTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACC                                                                             >  SRR4302547.1127561/1‑94 (MQ=60)

                 GTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACC                                                                             >  SRR4302547.4304890/1‑94 (MQ=60)

                  TCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCG                                                                            >  SRR4302547.4185840/1‑94 (MQ=60)

                   CATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGT                                                                           >  SRR4302547.739341/1‑94 (MQ=60)

                   CATGAATAGGGTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGAAGCCAGAAACTGCTCACGCGCAGGACCAACGATTGCTTCAACCGT                                                                           >  SRR4302547.647481/1‑94 (MQ=50)

                    ATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTC                                                                          <  SRR4302547.552204/94‑1 (MQ=60)

                    ATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTC                                                                          <  SRR4302547.157296/94‑1 (MQ=60)

                    ATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTC                                                                          >  SRR4302547.6607406/1‑94 (MQ=60)

                      GAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGG                                                                        <  SRR4302547.5622310/94‑1 (MQ=60)

                      GAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGG                                                                        >  SRR4302547.1254466/1‑94 (MQ=60)

                      GAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGG                                                                        >  SRR4302547.969063/1‑94 (MQ=60)

                        ATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCG                                                                      >  SRR4302547.5724584/1‑94 (MQ=60)

                          CGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTT                                                                    <  SRR4302547.2469645/94‑1 (MQ=60)

                          CGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTT                                                                    >  SRR4302547.4749869/1‑94 (MQ=60)

                          CGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTT                                                                    <  SRR4302547.5903247/94‑1 (MQ=60)

                           GCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTA                                                                   <  SRR4302547.3160810/94‑1 (MQ=60)

                            CCTCCCGTGGGATGGCTTCCCCGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAA                                                                  >  SRR4302547.6631803/1‑94 (MQ=60)

                            CCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCAATGCTTCAACCGTCGGCGTTAA                                                                  >  SRR4302547.1670638/1‑94 (MQ=60)

                            CCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAA                                                                  >  SRR4302547.1467218/1‑94 (MQ=60)

                            CCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAA                                                                  <  SRR4302547.4924628/94‑1 (MQ=60)

                            CATCCCGTGGGAGGGCTTCCACGCCAGATTCACGCAGCGCACCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAA                                                                  <  SRR4302547.2701256/94‑1 (MQ=60)

                             CTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAG                                                                 >  SRR4302547.3428518/1‑94 (MQ=60)

                             CTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAG                                                                 <  SRR4302547.2515978/94‑1 (MQ=60)

                               CCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCC                                                               >  SRR4302547.3248241/1‑94 (MQ=60)

                               CCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCC                                                               <  SRR4302547.599358/94‑1 (MQ=60)

                                CCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCG                                                              <  SRR4302547.838269/94‑1 (MQ=60)

                                 CGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGA                                                             >  SRR4302547.2936281/1‑94 (MQ=60)

                                 CGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGA                                                             <  SRR4302547.236839/94‑1 (MQ=60)

                                 CGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGA                                                             >  SRR4302547.5194150/1‑94 (MQ=60)

                                  GTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAT                                                            <  SRR4302547.6091535/94‑1 (MQ=60)

                                  GTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAT                                                            >  SRR4302547.5802416/1‑94 (MQ=60)

                                  GTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAT                                                            <  SRR4302547.133282/94‑1 (MQ=60)

                                   TGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATG                                                           >  SRR4302547.1335306/1‑94 (MQ=60)

                                   TGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATG                                                           >  SRR4302547.4723151/1‑94 (MQ=60)

                                    GGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGT                                                          >  SRR4302547.6603596/1‑94 (MQ=60)

                                     GGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTG                                                         >  SRR4302547.1602078/1‑94 (MQ=60)

                                      GATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGT                                                        <  SRR4302547.3613650/94‑1 (MQ=60)

                                       ATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTG                                                       >  SRR4302547.5683934/1‑94 (MQ=60)

                                       ATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTG                                                       <  SRR4302547.5179641/94‑1 (MQ=60)

                                       ATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTG                                                       <  SRR4302547.4249439/94‑1 (MQ=60)

                                        TGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGG                                                      >  SRR4302547.4619184/1‑94 (MQ=60)

                                         GGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGC                                                     >  SRR4302547.4834079/1‑94 (MQ=60)

                                          GCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCT                                                    >  SRR4302547.1743080/1‑94 (MQ=60)

                                          GCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCT                                                    <  SRR4302547.2066891/94‑1 (MQ=60)

                                           CTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTC                                                   >  SRR4302547.2976001/1‑94 (MQ=60)

                                           CTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTC                                                   <  SRR4302547.983790/94‑1 (MQ=60)

                                            TTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCC                                                  <  SRR4302547.6766559/94‑1 (MQ=60)

                                             TCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCA                                                 <  SRR4302547.1042845/94‑1 (MQ=60)

                                               CACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGC                                               <  SRR4302547.5921246/94‑1 (MQ=60)

                                                ACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCG                                              <  SRR4302547.5907943/94‑1 (MQ=60)

                                                  GCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAT                                            <  SRR4302547.1114756/94‑1 (MQ=60)

                                                  GCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAT                                            <  SRR4302547.4614647/94‑1 (MQ=60)

                                                     AGATTCACGCAGCGCAGCCAGAACCTGTTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCC                                         <  SRR4302547.5424118/94‑1 (MQ=60)

                                                     AGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCC                                         >  SRR4302547.1054804/1‑94 (MQ=60)

                                                     AGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCC                                         >  SRR4302547.2072897/1‑94 (MQ=60)

                                                     AGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCC                                         <  SRR4302547.6244819/94‑1 (MQ=60)

                                                      GATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCG                                        <  SRR4302547.2269019/94‑1 (MQ=60)

                                                      GATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCG                                        <  SRR4302547.4309890/94‑1 (MQ=60)

                                                        TTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGG                                      <  SRR4302547.3689018/94‑1 (MQ=60)

                                                        TTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGG                                      <  SRR4302547.1063598/94‑1 (MQ=60)

                                                         TCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGA                                     >  SRR4302547.2624060/1‑94 (MQ=60)

                                                          CACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGAT                                    <  SRR4302547.757046/94‑1 (MQ=60)

                                                           ACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATC                                   <  SRR4302547.3821853/94‑1 (MQ=60)

                                                            CGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCA                                  >  SRR4302547.6079792/1‑94 (MQ=60)

                                                             GCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCAC                                 <  SRR4302547.914654/94‑1 (MQ=60)

                                                             GCAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCAC                                 <  SRR4302547.4461933/94‑1 (MQ=60)

                                                              CAGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACG                                >  SRR4302547.2216864/1‑94 (MQ=60)

                                                               AGCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGC                               >  SRR4302547.5994745/1‑94 (MQ=60)

                                                                GCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCC                              <  SRR4302547.5471636/94‑1 (MQ=60)

                                                                GCGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCC                              >  SRR4302547.4495730/1‑94 (MQ=60)

                                                                 CGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCG                             <  SRR4302547.745761/94‑1 (MQ=60)

                                                                 CGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCG                             >  SRR4302547.2910732/1‑94 (MQ=60)

                                                                 CGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCG                             <  SRR4302547.3198721/94‑1 (MQ=60)

                                                                 CGCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCG                             >  SRR4302547.5694435/1‑94 (MQ=60)

                                                                  GCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGA                            <  SRR4302547.5176708/94‑1 (MQ=60)

                                                                  GCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGA                            <  SRR4302547.2940042/94‑1 (MQ=60)

                                                                  GCAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGA                            <  SRR4302547.1478092/94‑1 (MQ=60)

                                                                   CAGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAC                           <  SRR4302547.706840/94‑1 (MQ=60)

                                                                    AGCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACC                          >  SRR4302547.3212297/1‑94 (MQ=60)

                                                                     GCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCA                         <  SRR4302547.6247486/94‑1 (MQ=60)

                                                                     GCCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCA                         <  SRR4302547.3160217/94‑1 (MQ=60)

                                                                      CCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAG                        <  SRR4302547.2486751/94‑1 (MQ=60)

                                                                      CCAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAG                        >  SRR4302547.4017368/1‑94 (MQ=60)

                                                                       CAGAACCTGCTGAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGG                       <  SRR4302547.1359447/94‑1 (MQ=60)

                                                                       CAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGG                       >  SRR4302547.4366363/1‑94 (MQ=60)

                                                                       CAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGG                       >  SRR4302547.381147/1‑94 (MQ=60)

                                                                       CAGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGG                       <  SRR4302547.4460728/94‑1 (MQ=60)

                                                                        AGAACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGG                      <  SRR4302547.4043968/94‑1 (MQ=60)

                                                                          AACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTC                    >  SRR4302547.769454/1‑94 (MQ=60)

                                                                          AACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTC                    <  SRR4302547.3305368/94‑1 (MQ=60)

                                                                           ACCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCA                   >  SRR4302547.1025613/1‑94 (MQ=60)

                                                                            CCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAG                  <  SRR4302547.531513/94‑1 (MQ=60)

                                                                            CCTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAG                  >  SRR4302547.3618342/1‑94 (MQ=60)

                                                                             CTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGC                 >  SRR4302547.1711421/1‑94 (MQ=60)

                                                                             CTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGC                 >  SRR4302547.6175538/1‑94 (MQ=60)

                                                                             CTGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGC                 <  SRR4302547.4710897/94‑1 (MQ=60)

                                                                              TGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCT                >  SRR4302547.6111820/1‑94 (MQ=60)

                                                                              TGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCT                >  SRR4302547.641991/1‑94 (MQ=60)

                                                                              TGCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCT                <  SRR4302547.3841004/94‑1 (MQ=60)

                                                                               GCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTT               <  SRR4302547.723157/94‑1 (MQ=60)

                                                                               GCTCAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTT               >  SRR4302547.4697880/1‑94 (MQ=60)

                                                                                  CAAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTT            >  SRR4302547.4372736/1‑94 (MQ=60)

                                                                                   AAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTC           >  SRR4302547.6183529/1‑94 (MQ=60)

                                                                                   AAGCGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTC           >  SRR4302547.4854713/1‑94 (MQ=60)

                                                                                    AGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCG          >  SRR4302547.4910345/1‑94 (MQ=60)

                                                                                     GCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGG         <  SRR4302547.5859907/94‑1 (MQ=60)

                                                                                      CGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGA        >  SRR4302547.6127489/1‑94 (MQ=60)

                                                                                      CGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGA        >  SRR4302547.997377/1‑94 (MQ=60)

                                                                                      CGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGA        >  SRR4302547.330176/1‑94 (MQ=60)

                                                                                      CGCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGA        >  SRR4302547.5320898/1‑94 (MQ=60)

                                                                                       GCAGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAA       <  SRR4302547.4291818/94‑1 (MQ=60)

                                                                                        CAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAA      >  SRR4302547.3488085/1‑94 (MQ=60)

                                                                                         AGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAAC     <  SRR4302547.988554/94‑1 (MQ=60)

                                                                                         AGGCTCAATCATTGCTTCACCCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAAC     <  SRR4302547.3813753/94‑1 (MQ=60)

                                                                                         AGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAAC     >  SRR4302547.4134911/1‑94 (MQ=60)

                                                                                         AGGCTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAAC     >  SRR4302547.2269348/1‑94 (MQ=60)

                                                                                          GGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACT    <  SRR4302547.4427666/94‑1 (MQ=60)

                                                                                          GGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACT    <  SRR4302547.5575812/94‑1 (MQ=60)

                                                                                            TTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCG  <  SRR4302547.1804359/94‑1 (MQ=60)

                                                                                            CTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCG  >  SRR4302547.2935263/1‑94 (MQ=60)

                                                                                            CTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCG  <  SRR4302547.1174704/94‑1 (MQ=60)

                                                                                            CTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCG  >  SRR4302547.5600371/1‑94 (MQ=60)

AACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCG  >  NC_000913/3138793‑3138978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`