Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,142,022 A→G V53A (GTG→GCG)  hybB ← putative cytochrome Ni/Fe component of hydrogenase‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,142,0220AG95.5% 558.7 / 9.8 179V53A (GTG→GCG) hybBputative cytochrome Ni/Fe component of hydrogenase‑2
Reads supporting (aligned to +/- strand):  ref base A (5/3);  new base G (74/97);  total (79/100)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 203 total aligned reads displayed.

TGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATT  >  NC_000913/3141932‑3142111
                                                                                           |                                                                                         
tGCCCACGGTTAAAGCCGTATCCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCAcgcg                                                                                         <  1:2044933/93‑1 (MQ=255)
tGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCAcgcg                                                                                         >  1:5684806/1‑93 (MQ=255)
  cccACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGcc                                                                                       >  2:2100740/1‑93 (MQ=255)
   ccACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGccc                                                                                      >  1:1207898/1‑93 (MQ=255)
   ccACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGccc                                                                                      >  2:3239996/1‑93 (MQ=255)
    cACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGcccc                                                                                     <  1:992954/93‑1 (MQ=255)
     ccGGTTAAAGACGTATACCGCCCCCGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCa                                                                                    <  2:2161886/92‑1 (MQ=255)
      cGGTTAAAGACGTATACCGCCCCCGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCAc                                                                                   <  2:397932/93‑1 (MQ=255)
      cGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCAc                                                                                   >  1:1670361/1‑93 (MQ=255)
      cGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCAc                                                                                   >  1:2460531/1‑93 (MQ=255)
        gTTAAAGACGTATCCCCCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACgg                                                                                 <  1:10673/93‑1 (MQ=255)
        gTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACgg                                                                                 <  1:700670/93‑1 (MQ=255)
        gTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACgg                                                                                 >  2:4795466/1‑93 (MQ=255)
        gTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACgg                                                                                 <  2:5775260/93‑1 (MQ=255)
           aaaGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGaa                                                                              <  2:1299288/93‑1 (MQ=255)
           aaaGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGaa                                                                              <  1:2893432/93‑1 (MQ=255)
             aGACGTATCCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAgc                                                                            <  1:223568/93‑1 (MQ=255)
                cGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAGACGCGATCCACGCGCCCCACGTGAAgccgc                                                                         >  1:5207543/1‑93 (MQ=255)
                cGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAgccgc                                                                         <  1:256197/93‑1 (MQ=255)
                 gTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAgccgcc                                                                        >  1:5624314/1‑93 (MQ=255)
                  tataCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGccgccg                                                                       >  1:6085894/1‑93 (MQ=255)
                  tataCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGccgccg                                                                       >  1:2675198/1‑93 (MQ=255)
                    taCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGtt                                                                     >  1:3467979/1‑93 (MQ=255)
                       cGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAg                                                                  >  1:3044286/1‑93 (MQ=255)
                       cGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAg                                                                  >  1:851789/1‑93 (MQ=255)
                        gCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAgg                                                                 >  1:3743739/1‑93 (MQ=255)
                        cccccCGCCGGCCCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAgg                                                                 <  1:5422334/92‑1 (MQ=255)
                          ccccGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTc                                                               <  2:4345274/90‑1 (MQ=255)
                          ccACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTc                                                               <  2:3872639/93‑1 (MQ=255)
                            ccGCCAGCCCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCag                                                             <  2:2981420/92‑1 (MQ=255)
                            aCGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCag                                                             >  1:4735420/1‑93 (MQ=255)
                            aCGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCag                                                             >  1:5998420/1‑93 (MQ=255)
                             cGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCaga                                                            >  1:2947657/1‑93 (MQ=255)
                             cGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCaga                                                            <  1:4540610/93‑1 (MQ=255)
                               ccAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAgaga                                                          <  2:753661/93‑1 (MQ=255)
                                cAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGAc                                                         <  2:2133289/93‑1 (MQ=255)
                                cAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGCTCAGAGAc                                                         >  2:790022/1‑93 (MQ=255)
                                    gcCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACagag                                                     <  2:3894031/93‑1 (MQ=255)
                                    gcCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACagag                                                     >  2:4582366/1‑93 (MQ=255)
                                    ccccAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACagag                                                     <  2:5663319/92‑1 (MQ=255)
                                     cccAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGc                                                    >  2:377647/1‑93 (MQ=255)
                                      ccAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGcc                                                   <  1:2603409/93‑1 (MQ=255)
                                      ccAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGcc                                                   <  1:3131774/93‑1 (MQ=255)
                                        aGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGccga                                                 >  2:1538122/1‑91 (MQ=255)
                                         gccgccACAGGCAAAGCCGG‑GGCCAATCAGCAGGTCAAACGCGATCCACGCCCCCCACGGGAGGCCGCCGTTAGGGTCAGAGACAGGCCCCAg                                                >  2:3709367/1‑93 (MQ=255)
                                           cgccACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGCGCCCAGAc                                              >  1:2819102/1‑93 (MQ=255)
                                           cgccACAGGC‑AAGCCGGCGGCCAACAGGCGGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGAc                                              <  1:100571/93‑1 (MQ=255)
                                            gccACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGAcc                                             >  1:4287113/1‑93 (MQ=255)
                                            cccACGGGCAAACCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGAcc                                             <  2:1379514/92‑1 (MQ=255)
                                                caGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAac                                         <  1:2318597/93‑1 (MQ=255)
                                                caGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAac                                         >  2:5211530/1‑93 (MQ=255)
                                                caGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAac                                         >  2:3035594/1‑93 (MQ=255)
                                                caGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAac                                         <  2:3383020/93‑1 (MQ=255)
                                                 aGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAaca                                        >  2:5441929/1‑93 (MQ=255)
                                                 aGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAaca                                        >  1:938082/1‑93 (MQ=255)
                                                  ggCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAacac                                       >  2:4141399/1‑93 (MQ=255)
                                                  ggCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAacac                                       >  1:4291876/1‑93 (MQ=255)
                                                     aaaGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAg                                    <  1:2718789/93‑1 (MQ=255)
                                                      aaGCCGG‑TGCCAATCAGCAGGTCAACCGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGa                                   <  1:1311958/93‑1 (MQ=255)
                                                      aaGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGa                                   <  2:1211518/93‑1 (MQ=255)
                                                       aGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGAc                                  <  1:3937934/93‑1 (MQ=255)
                                                       aGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGAc                                  >  1:4840078/1‑93 (MQ=255)
                                                        gCCGT‑TGCCAAACAGGATGTCAAACGCGGTCCACGCGCCCCACGGGAAGCCTCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACg                                 <  1:2107744/93‑1 (MQ=255)
                                                        gCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACg                                 >  2:5290404/1‑93 (MQ=255)
                                                               gCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTcac                           <  2:4330565/93‑1 (MQ=255)
                                                                 cAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACaa                         <  2:5906646/93‑1 (MQ=255)
                                                                 cAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACaa                         >  1:5739425/1‑93 (MQ=255)
                                                                  aaTCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAAt                        <  2:4856883/93‑1 (MQ=255)
                                                                  aaTCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAAt                        >  1:1973767/1‑93 (MQ=255)
                                                                   aTCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATc                       <  2:2641113/93‑1 (MQ=255)
                                                                   aTCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGCCAGAGCCCAGACCGAACACCAGACGCTTCACACTc                       >  2:3213081/1‑93 (MQ=255)
                                                                    tCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCa                      >  1:983186/1‑93 (MQ=255)
                                                                    tCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCa                      <  2:3808302/93‑1 (MQ=255)
                                                                        cagGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAg                  <  2:1264511/93‑1 (MQ=255)
                                                                        cagGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAg                  <  2:644076/93‑1 (MQ=255)
                                                                          ggTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCa                <  2:2062705/93‑1 (MQ=255)
                                                                           gTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCAt               >  1:3977317/1‑93 (MQ=255)
                                                                           gTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCAt               <  1:5944973/93‑1 (MQ=255)
                                                                             cAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATAc             >  2:2909967/1‑93 (MQ=255)
                                                                             cAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATAc             <  2:1366583/93‑1 (MQ=255)
                                                                              aaaCGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCCCAATCAGGAGCATACa            >  2:7022/1‑93 (MQ=255)
                                                                              aaaCGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACa            <  2:4542402/93‑1 (MQ=255)
                                                                              aaaCGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCACGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACa            <  2:1724417/93‑1 (MQ=255)
                                                                                aCGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGa          >  1:1385978/1‑93 (MQ=255)
                                                                                aCGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGa          <  2:5561053/93‑1 (MQ=255)
                                                                                aCGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGa          <  1:3111309/93‑1 (MQ=255)
                                                                                 cgcgATCCGCGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGAt         <  1:2749161/93‑1 (MQ=255)
                                                                                 cgcgATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGAt         <  2:2347394/93‑1 (MQ=255)
                                                                                  gcgATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATg        <  1:606743/93‑1 (MQ=255)
                                                                                   cgATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGa       <  2:5606062/93‑1 (MQ=255)
                                                                                    gATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAc      >  1:3088632/1‑93 (MQ=255)
                                                                                    gATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAc      <  1:5035161/93‑1 (MQ=255)
                                                                                    gATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAc      <  2:4029249/93‑1 (MQ=255)
                                                                                    gATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAc      >  1:1841515/1‑93 (MQ=255)
                                                                                    gATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAc      <  2:152490/93‑1 (MQ=255)
                                                                                      tCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGa    >  2:1422728/1‑93 (MQ=255)
                                                                                      tCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGa    >  2:845525/1‑93 (MQ=255)
                                                                                      accACGCGCCCCACGGAAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGa    <  1:3609974/92‑1 (MQ=255)
                                                                                       ccACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGAt   <  1:4323174/93‑1 (MQ=255)
                                                                                       ccACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGAt   <  2:2712001/93‑1 (MQ=255)
                                                                                           |                                                                                         
TGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGG‑TGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATT  >  NC_000913/3141932‑3142111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACG  >  NC_000913/3141929‑3142115
                                                                                             |                                                                                             
TATTGCCCCCGGTTAAAGACGTATCCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACG                                                                                               <  SRR4302532.3280144/94‑1 (MQ=60)
TATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCTATCCACG                                                                                               <  SRR4302532.4287388/94‑1 (MQ=60)
TATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACG                                                                                               >  SRR4302532.2771231/1‑94 (MQ=60)
  TTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCG                                                                                             <  SRR4302532.550246/94‑1 (MQ=60)
  TTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCG                                                                                             >  SRR4302532.5883244/1‑94 (MQ=60)
   TGCCCACGGTTAAAGCCGTATCCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGC                                                                                            <  SRR4302532.2113027/94‑1 (MQ=60)
     CCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCC                                                                                          >  SRR4302532.1248324/1‑94 (MQ=60)
       CACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCA                                                                                        >  SRR4302532.3086848/1‑94 (MQ=60)
       CACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCA                                                                                        <  SRR4302532.1026834/94‑1 (MQ=60)
        ACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCAC                                                                                       >  SRR4302532.1726884/1‑94 (MQ=60)
        ACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCAC                                                                                       >  SRR4302532.2542392/1‑94 (MQ=60)
         CGGTTAAAGACGTATCCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACG                                                                                      <  SRR4302532.2679415/94‑1 (MQ=60)
         CGGATAAAGACGTATACCGCCAACGCCCGCGCACAGCCGCCACAGGCAAAGCCGGTGCCACTCAGCAGGTCAAACGCGATCCACGCGCCCCACG                                                                                      >  SRR4302532.5605542/1‑94 (MQ=60)
           gttaaagactattccccCCCACGCAAGCGCCCAGCCTCCACAGGCAAAGCCTTTGCTAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGG                                                                                    <  SRR4302532.2731815/77‑1 (MQ=58)
           GTTAAAGACGTATCCCCCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGG                                                                                    <  SRR4302532.11128/94‑1 (MQ=60)
           GTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGG                                                                                    <  SRR4302532.3030313/94‑1 (MQ=60)
           GTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGG                                                                                    >  SRR4302532.4161538/1‑94 (MQ=60)
           GTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGG                                                                                    <  SRR4302532.724634/94‑1 (MQ=60)
             TAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAA                                                                                  >  SRR4302532.6280498/1‑94 (MQ=60)
             TAAAGACGTACACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAA                                                                                  <  SRR4302532.6243177/94‑1 (MQ=60)
              AAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAG                                                                                 <  SRR4302532.2990058/94‑1 (MQ=60)
                AGACGTATCCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCC                                                                               <  SRR4302532.231203/94‑1 (MQ=60)
                AGACGTATACCGCCCCCGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCC                                                                               <  SRR4302532.5091546/94‑1 (MQ=60)
                  ACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAGACGCGATCCACGCGCCCCACGTGAAGCCGC                                                                             >  SRR4302532.5387126/1‑94 (MQ=60)
                   ctgATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCC                                                                            <  SRR4302532.265089/91‑1 (MQ=60)
                   CGTATCCCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCC                                                                            <  SRR4302532.5648122/94‑1 (MQ=60)
                   CGTATACCGCCCACGCCAGCGCCCAGCCGCCCCAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCC                                                                            >  SRR4302532.1618525/1‑94 (MQ=60)
                   CGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCC                                                                            <  SRR4302532.265090/94‑1 (MQ=60)
                   CGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCC                                                                            >  SRR4302532.2986467/1‑94 (MQ=60)
                   CGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCC                                                                            <  SRR4302532.5959770/94‑1 (MQ=60)
                   CGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCC                                                                            >  SRR4302532.5819489/1‑94 (MQ=60)
                    GTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCG                                                                           >  SRR4302532.2764990/1‑94 (MQ=60)
                    GTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCG                                                                           >  SRR4302532.6296030/1‑94 (MQ=60)
                      ATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTT                                                                         >  SRR4302532.3584118/1‑94 (MQ=60)
                       cgccccccagcCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAACCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTC                                                                        <  SRR4302532.4940274/83‑1 (MQ=60)
                         CCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAG                                                                      >  SRR4302532.3144748/1‑94 (MQ=60)
                         CCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAG                                                                      >  SRR4302532.881089/1‑94 (MQ=60)
                          CGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGG                                                                     >  SRR4302532.3869572/1‑94 (MQ=60)
                           ccccccgccggccCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGT                                                                    <  SRR4302532.5610273/81‑1 (MQ=60)
                              CACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAG                                                                 >  SRR4302532.4897083/1‑94 (MQ=60)
                              CACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAG                                                                 >  SRR4302532.6205602/1‑94 (MQ=60)
                               ACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGA                                                                >  SRR4302532.3045432/1‑94 (MQ=60)
                                CGCCGGCGCCCACCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAG                                                               <  SRR4302532.2122670/94‑1 (MQ=60)
                                CGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAG                                                               >  SRR4302532.4808487/1‑94 (MQ=60)
                                CGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAG                                                               <  SRR4302532.4695607/94‑1 (MQ=60)
                                CGCAAGCCCCCAGCCGCCACAGGCAACGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAG                                                               <  SRR4302532.3194915/94‑1 (MQ=60)
                                    AGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAG                                                           >  SRR4302532.3840614/1‑94 (MQ=60)
                                      CGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAG                                                         <  SRR4302532.2191282/94‑1 (MQ=60)
                                       GCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGC                                                        >  SRR4302532.1763262/1‑94 (MQ=60)
                                       GCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGC                                                        >  SRR4302532.4704512/1‑94 (MQ=60)
                                         CCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCC                                                      <  SRR4302532.3235738/94‑1 (MQ=60)
                                         CCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCC                                                      >  SRR4302532.1016172/1‑94 (MQ=60)
                                         CCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCC                                                      <  SRR4302532.2690472/94‑1 (MQ=60)
                                             CCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGCGCCCAGAC                                                  >  SRR4302532.2913449/1‑94 (MQ=60)
                                              cgccacaggcaagccggcggccaacaggcgGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACC                                                 <  SRR4302532.104062/64‑1 (MQ=60)
                                              CGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACC                                                 >  SRR4302532.4431700/1‑94 (MQ=60)
                                                  ACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAAC                                             >  SRR4302532.3155219/1‑94 (MQ=60)
                                                  ACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAAC                                             <  SRR4302532.2317640/94‑1 (MQ=60)
                                                   CAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACA                                            >  SRR4302532.970231/1‑94 (MQ=60)
                                                   CAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACA                                            <  SRR4302532.2395774/94‑1 (MQ=60)
                                                   CAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACA                                            >  SRR4302532.1351239/1‑94 (MQ=60)
                                                    AGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACAC                                           >  SRR4302532.4436763/1‑94 (MQ=60)
                                                    AGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACAC                                           >  SRR4302532.4436762/1‑94 (MQ=60)
                                                       CAAAGCCGGTCCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAG                                        <  SRR4302532.2867570/94‑1 (MQ=60)
                                                        AAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCTAGACCGAACACCAGA                                       <  SRR4302532.4091466/94‑1 (MQ=60)
                                                        AAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGA                                       <  SRR4302532.3306641/94‑1 (MQ=60)
                                                        AAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGA                                       >  SRR4302532.785083/1‑94 (MQ=60)
                                                        AAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGA                                       <  SRR4302532.2810232/94‑1 (MQ=60)
                                                         AAGCCGGTGCCAATCAGCAGGTCAACCGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGAC                                      <  SRR4302532.1355849/94‑1 (MQ=60)
                                                         AAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGAC                                      >  SRR4302532.5006714/1‑94 (MQ=60)
                                                         AAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGAC                                      >  SRR4302532.3922232/1‑94 (MQ=60)
                                                          AGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACG                                     <  SRR4302532.4070631/94‑1 (MQ=60)
                                                           gcctttgccaagcacaAGGTCAAACGCGATCCACGCCCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGC                                    <  SRR4302532.4694792/78‑1 (MQ=60)
                                                           GCCGTTGCCAAACAGGATGTCAAACGCGGTCCACGCGCCCCACGGGAAGCCTCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGC                                    <  SRR4302532.2178093/94‑1 (MQ=60)
                                                             ctcTGCCAATCACCAGGTCAAACGCCATCCACCCGCCCCACGGGAACCCCCCGTTCAGGTCAGAGACAGACCCCAGACCGAACCCCAGACGCTT                                  <  SRR4302532.1968769/91‑1 (MQ=60)
                                                             CGGTCCCAAGCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTT                                  <  SRR4302532.1968768/94‑1 (MQ=60)
                                                             CGGGGCCAATCTGCTGGTCAAACGCGATCCATGCGTTCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAATACCAGACGCTC                                  >  SRR4302532.4916976/1‑94 (MQ=60)
                                                               GTGCCAATCAGCAGTTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCA                                <  SRR4302532.708107/94‑1 (MQ=60)
                                                               GTGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCA                                <  SRR4302532.1058462/94‑1 (MQ=60)
                                                                TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCAC                               >  SRR4302532.5608900/1‑94 (MQ=60)
                                                                TGCCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCAC                               <  SRR4302532.2775548/94‑1 (MQ=60)
                                                                  CCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGTGAAGACGCCGTTCAGGTCATAGACAGAGCCCAGACCGAACACAAGACGCTTCACAA                             >  SRR4302532.2391993/1‑94 (MQ=60)
                                                                  CCAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAA                             >  SRR4302532.5939244/1‑94 (MQ=60)
                                                                   CAATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAAT                            >  SRR4302532.2039331/1‑94 (MQ=60)
                                                                   CAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAAT                            >  SRR4302532.5866957/1‑94 (MQ=60)
                                                                     ATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCA                          <  SRR4302532.88980/94‑1 (MQ=60)
                                                                     ATCAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCA                          >  SRR4302532.1016762/1‑94 (MQ=60)
                                                                       CAGCAGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGG                        <  SRR4302532.4431878/94‑1 (MQ=60)
                                                                           AGGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCA                    >  SRR4302532.1431094/1‑94 (MQ=60)
                                                                            GGTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCAT                   >  SRR4302532.4111297/1‑94 (MQ=60)
                                                                             GTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATA                  <  SRR4302532.2416048/94‑1 (MQ=60)
                                                                             GTCAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATA                  <  SRR4302532.6150667/94‑1 (MQ=60)
                                                                              TCAACCGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATAC                 <  SRR4302532.283955/94‑1 (MQ=60)
                                                                               CAAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACA                <  SRR4302532.1173684/94‑1 (MQ=60)
                                                                                AAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAG               >  SRR4302532.3642368/1‑94 (MQ=60)
                                                                                AAACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAG               <  SRR4302532.3231581/94‑1 (MQ=60)
                                                                                AAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAG               <  SRR4302532.1018372/94‑1 (MQ=60)
                                                                                 AACGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGA              >  SRR4302532.1432416/1‑94 (MQ=60)
                                                                                  ACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGAT             <  SRR4302532.3214517/94‑1 (MQ=60)
                                                                                   CGCGATCCGCGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATG            <  SRR4302532.2841492/94‑1 (MQ=60)
                                                                                   CGCGATCCACGCGCCCCCCCGGAAGCCGCCGGTCAGGTCAGAGACAGAGCCCCAACCGAACACCAGACGCTTCCCCATCAGGAGCCTACAGAAG            >  SRR4302532.1049942/1‑94 (MQ=48)
                                                                                   CGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATG            <  SRR4302532.6154874/94‑1 (MQ=60)
                                                                                   CGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATG            >  SRR4302532.4513235/1‑94 (MQ=60)
                                                                                   CGCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATG            >  SRR4302532.2040204/1‑94 (MQ=60)
                                                                                    GCGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGA           <  SRR4302532.627927/94‑1 (MQ=60)
                                                                                     CGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAC          <  SRR4302532.5917037/94‑1 (MQ=60)
                                                                                     CGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAC          >  SRR4302532.3190935/1‑94 (MQ=60)
                                                                                     CGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAC          >  SRR4302532.1902689/1‑94 (MQ=60)
                                                                                     CGATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGAC          <  SRR4302532.5254183/94‑1 (MQ=60)
                                                                                      GATCCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACG         <  SRR4302532.5207848/94‑1 (MQ=60)
                                                                                        ACCACGCGCCCCACGGAAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGAT       <  SRR4302532.3730668/94‑1 (MQ=60)
                                                                                         CCACGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATT      <  SRR4302532.4469272/94‑1 (MQ=60)
                                                                                            CGCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAAC   <  SRR4302532.3350764/94‑1 (MQ=60)
                                                                                             GCGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACG  <  SRR4302532.2344105/94‑1 (MQ=60)
                                                                                             |                                                                                             
TATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAGCCGCCACAGGCAAAGCCGGTGCCAATCAGCAGGTCAAACGCGATCCACACGCCCCACGGGAAGCCGCCGTTCAGGTCAGAGACAGAGCCCAGACCGAACACCAGACGCTTCACAATCAGGAGCATACAGATGACGATTAACG  >  NC_000913/3141929‑3142115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑