Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,130,334	A→G	V30V (GTT→GTC)	b2983 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,130,334	0	A	G	100.0%	713.7 / NA	192	V30V (GTT→GTC)	b2983	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (90/102); total (90/102)

Only 100 of 210 total aligned reads displayed.

CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA  >  NC_000913/3130244‑3130422

cGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCacgac                                                                                        >  2:4094837/1‑93 (MQ=255)

cGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCacgac                                                                                        <  1:467420/93‑1 (MQ=255)

cGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCacgac                                                                                        <  2:5532152/93‑1 (MQ=255)

 gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACACCGCAAAGCGCTCacaaca                                                                                       >  1:216445/1‑93 (MQ=255)

  aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCCGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAt                                                                                      >  2:2140947/1‑93 (MQ=255)

  aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCCGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAt                                                                                      >  2:636846/1‑93 (MQ=255)

     gCACAATCCCCAGTACGCAAAACGTGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCACCAACAGACCCAGCAAGGCGCTCACGACATTAc                                                                                   <  2:4890343/93‑1 (MQ=255)

     gCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCACCAAGGCGCTCACGACATTAc                                                                                   >  1:5452757/1‑93 (MQ=255)

      cacaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACt                                                                                  <  1:1029381/93‑1 (MQ=255)

       acaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCCGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTg                                                                                 >  1:2769870/1‑93 (MQ=255)

        caATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTgg                                                                                <  2:2075710/93‑1 (MQ=255)

         aaTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGa                                                                               <  1:1004663/93‑1 (MQ=255)

          aTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCCACAGACCCAGCAAGGCGCTCACGACATTACTCGAg                                                                              >  2:2627056/1‑93 (MQ=255)

          aTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAg                                                                              <  1:753768/93‑1 (MQ=255)

          aTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGAACCAGCAAGGCGCTCACGACATTACTGGAg                                                                              >  1:1175556/1‑93 (MQ=255)

           tCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGc                                                                             <  2:5339223/93‑1 (MQ=255)

           tCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGAGCTCACGACATTACTGGAGc                                                                             <  1:6399327/93‑1 (MQ=255)

            cACCAGTACGCAAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGcc                                                                            <  2:3731516/93‑1 (MQ=255)

             aCCAGTACGCAAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCt                                                                           <  2:4016390/93‑1 (MQ=255)

              ccAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTa                                                                          <  1:2982709/93‑1 (MQ=255)

              ccAGTACGCCAAACATGGCGGGCGGCATCCCTTTA‑CCGGCACACGCGAGCGGCAAAAGACCCCACAAAGAGCTCCCGACATTACTGGGGccga                                                                          >  2:4224391/1‑91 (MQ=255)

                 gTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAcc                                                                       >  1:3002506/1‑93 (MQ=255)

                   cccccAAACGGGGGGCGCGTCCCCCTTTTC‑CCGCCACCGGAGCGCCCCAAAGGCCCCAGAAAGGCCCCCACGACATTACTGGAGCCTAACCAg                                                                     <  2:114003/90‑1 (MQ=255)

                   aCGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAg                                                                     <  2:3108201/93‑1 (MQ=255)

                   aCGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAg                                                                     >  1:5416962/1‑93 (MQ=255)

                    cGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAgg                                                                    <  2:2872975/93‑1 (MQ=255)

                      ccAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcg                                                                  >  1:5618909/1‑93 (MQ=255)

                       cAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                                 >  2:6345489/1‑93 (MQ=255)

                       cAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                                 >  2:483831/1‑93 (MQ=255)

                        aaaCATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCt                                                                <  1:4387183/93‑1 (MQ=255)

                        aaaCATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAAAAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCt                                                                >  2:4308448/1‑93 (MQ=255)

                         aaCATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTa                                                               <  1:3046349/93‑1 (MQ=255)

                            aTGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTg                                                            <  1:953051/93‑1 (MQ=255)

                             tGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGc                                                           <  1:3244502/93‑1 (MQ=255)

                             gggcgggcgTCACCCCTTTC‑CCGCCACACGAGAGCCCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGc                                                           <  1:1463185/92‑1 (MQ=255)

                               gcgggcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGa                                                         <  1:2810388/93‑1 (MQ=255)

                               gcgggcgCCACCCCTTTC‑CCGGCACACGAGAGCGCAAACAGCCCCAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGa                                                         <  1:2573433/93‑1 (MQ=255)

                                   gcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                     >  2:4857181/1‑93 (MQ=255)

                                   gcgTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                     >  1:6414453/1‑93 (MQ=255)

                                     gTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                   <  2:606329/93‑1 (MQ=255)

                                     gTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                   <  1:638368/93‑1 (MQ=255)

                                     cctctCCCTTTCCCCGGCACAGGAGAGCCCCAAAAGCCCCGGAAGGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                    <  1:2484962/89‑1 (MQ=255)

                                      tCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                  >  2:766824/1‑93 (MQ=255)

                                      tCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                  <  2:2100468/93‑1 (MQ=255)

                                      tCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                  >  2:5192199/1‑93 (MQ=255)

                                       cATCCCTTTC‑CCGCCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                 <  2:234061/93‑1 (MQ=255)

                                        aTCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                                >  1:6364356/1‑93 (MQ=255)

                                        aTCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                                >  1:5187497/1‑93 (MQ=255)

                                         tCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                               >  2:3542572/1‑93 (MQ=255)

                                         ccccTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                               <  2:2817506/92‑1 (MQ=255)

                                          cccTTTC‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCg                                              <  1:4181825/93‑1 (MQ=255)

                                             tttA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                           >  1:1539640/1‑93 (MQ=255)

                                              ttA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                          <  2:4057910/93‑1 (MQ=255)

                                              ttA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                          <  2:897061/93‑1 (MQ=255)

                                               tA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                                         <  2:3222868/93‑1 (MQ=255)

                                                  cggccacaCGAGAGCGCAAAAAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgcg                                       <  2:5923205/89‑1 (MQ=255)

                                                       acacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                  <  1:1656075/93‑1 (MQ=255)

                                                       acacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                  >  1:3996591/1‑93 (MQ=255)

                                                       acacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                  >  1:2002917/1‑93 (MQ=255)

                                                        cacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                                 <  1:4431549/93‑1 (MQ=255)

                                                         acGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTg                                >  1:6189584/1‑93 (MQ=255)

                                                          cGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGc                               >  2:6623218/1‑93 (MQ=255)

                                                           gagaGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCa                              >  1:1772413/1‑93 (MQ=255)

                                                           gagaGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCa                              >  2:2227294/1‑93 (MQ=255)

                                                            agagCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa                             <  2:643669/93‑1 (MQ=255)

                                                            agagCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa                             >  2:4169644/1‑93 (MQ=255)

                                                             gagCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaaa                            <  2:6244026/93‑1 (MQ=255)

                                                                cgcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCa                         <  1:6607143/93‑1 (MQ=255)

                                                                 gcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCaa                        <  2:2676454/93‑1 (MQ=255)

                                                                  ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                       >  1:837889/1‑93 (MQ=255)

                                                                  ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                       <  2:6562299/93‑1 (MQ=255)

                                                                  ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                       >  1:5595840/1‑93 (MQ=255)

                                                                   cAACAGACCGAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATg                      <  1:3637054/93‑1 (MQ=255)

                                                                    aaCAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt                     <  2:2488669/93‑1 (MQ=255)

                                                                    aaCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt                     >  2:4572749/1‑93 (MQ=255)

                                                                     aCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGtt                    >  1:3427296/1‑93 (MQ=255)

                                                                     aCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGtt                    <  1:6152600/93‑1 (MQ=255)

                                                                     aCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGtt                    >  2:3347709/1‑93 (MQ=255)

                                                                     aCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGtt                    <  2:2968111/93‑1 (MQ=255)

                                                                       aGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACTAATGTTTg                  >  2:1821636/1‑93 (MQ=255)

                                                                       aGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTg                  >  2:2544690/1‑93 (MQ=255)

                                                                        aaCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                 <  2:4205017/92‑1 (MQ=255)

                                                                         aCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAt                >  1:5084471/1‑93 (MQ=255)

                                                                            cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGtt             >  2:3634778/1‑93 (MQ=255)

                                                                            cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGtt             >  1:1488659/1‑93 (MQ=255)

                                                                             aGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTa            >  2:1726737/1‑93 (MQ=255)

                                                                               cAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa          <  1:1826709/93‑1 (MQ=255)

                                                                                aaGGCACTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaaa         <  2:4768890/93‑1 (MQ=255)

                                                                                 aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc        >  1:3900517/1‑93 (MQ=255)

                                                                                 aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc        >  2:4156853/1‑93 (MQ=255)

                                                                                  ggCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc       >  1:677499/1‑93 (MQ=255)

                                                                                  ggCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc       >  2:4264106/1‑93 (MQ=255)

                                                                                  ggCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc       >  2:1883635/1‑93 (MQ=255)

                                                                                   gcgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAccc      <  2:1501917/93‑1 (MQ=255)

                                                                                     gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGc    >  1:913422/1‑93 (MQ=255)

                                                                                      cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGcc   <  2:5044030/93‑1 (MQ=255)

                                                                                      cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGcc   >  1:5907500/1‑93 (MQ=255)

                                                                                      cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGcc   <  2:1433456/93‑1 (MQ=255)

                                                                                       tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa  >  2:5140019/1‑93 (MQ=255)

                                                                                       tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa  >  1:433232/1‑93 (MQ=255)

CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA  >  NC_000913/3130244‑3130422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427

GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑ACGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACG                                                                                               >  SRR4302547.1899793/1‑94 (MQ=60)

 TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCCGCAAGGCGCTCACGA                                                                                              >  SRR4302547.197617/1‑94 (MQ=60)

 TACGATTCCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTC‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAGGGCGCTCACGA                                                                                              <  SRR4302547.1390333/94‑1 (MQ=60)

  ACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGAC                                                                                             <  SRR4302547.1998892/94‑1 (MQ=60)

   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACA                                                                                            <  SRR4302547.476031/94‑1 (MQ=60)

   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACACCGCAAAGCGCTCACAACA                                                                                            >  SRR4302547.220527/1‑94 (MQ=60)

   CGACTGCACAATCACAAGTACACCAAACATGGCGGGCGTCAGCCCTTCA‑CCGGCACACGAGGCCGCCATCAGCCCCAGCAAGGCGCCCACGACA                                                                                            <  SRR4302547.4311663/94‑1 (MQ=38)

     ATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATT                                                                                          >  SRR4302547.5505243/1‑94 (MQ=60)

     ATTGCACAACCACCAGTACGCAAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATT                                                                                          <  SRR4302547.562537/94‑1 (MQ=60)

       TGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCACCAAGGCGCTCACGACATTAC                                                                                        >  SRR4302547.5565433/1‑94 (MQ=60)

        GCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACT                                                                                       <  SRR4302547.6565940/94‑1 (MQ=60)

         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTG                                                                                      <  SRR4302547.1049602/94‑1 (MQ=60)

         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCCGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTG                                                                                      >  SRR4302547.2825365/1‑94 (MQ=60)

          ACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGAAATTACTGG                                                                                     >  SRR4302547.4705881/1‑94 (MQ=60)

            AATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAG                                                                                   <  SRR4302547.1024263/94‑1 (MQ=60)

            AATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGAACCAGCAAGGCGCTCACGACATTACTGGAG                                                                                   >  SRR4302547.1199311/1‑94 (MQ=60)

             ATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGC                                                                                  <  SRR4302547.767977/94‑1 (MQ=60)

             ATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGC                                                                                  <  SRR4302547.868828/94‑1 (MQ=60)

              TCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCC                                                                                 <  SRR4302547.2144070/94‑1 (MQ=60)

              TCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGAGCTCACGACATTACTGGAGCC                                                                                 <  SRR4302547.6532816/94‑1 (MQ=60)

               CACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCTGCACACGAAAGCGCCCACAGACCCACCAAGGCGCTCACGACACTACCGGAGCCT                                                                                >  SRR4302547.5636490/1‑94 (MQ=60)

                 CCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAA                                                                              <  SRR4302547.3042072/94‑1 (MQ=60)

                 CCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAA                                                                              >  SRR4302547.6288190/1‑94 (MQ=60)

                  CAGTACGCCAAACATGGCGGGCGCCATCCCTTTA‑CTGGCACACTAGAGAGCCAACAGAGCCAGCTAGGCGCTAGCGAAAATACTGGAGCCTAAC                                                                             >  SRR4302547.4886349/1‑94 (MQ=38)

                   agtccacaaaacgTGGCGGGCTTCACCCCTTCA‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAGGGCGCCCACGACATTACTGGAGCCTAACC                                                                            <  SRR4302547.3771800/81‑1 (MQ=53)

                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            >  SRR4302547.3062221/1‑94 (MQ=60)

                     TACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAG                                                                          <  SRR4302547.1804751/94‑1 (MQ=60)

                     TACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAG                                                                          >  SRR4302547.5529021/1‑94 (MQ=60)

                       CGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGC                                                                        >  SRR4302547.15421/1‑94 (MQ=60)

                        ccaAAACAGGGCGGGCGTCACCCCTTTC‑CCGGCACACGAGAGCGCAAACAGCCCCAGAAGGCCGCCCACGACATTACTGGAGCCTAACCAGGCG                                                                       <  SRR4302547.4949940/91‑1 (MQ=37)

                        GCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCG                                                                       >  SRR4302547.5734871/1‑94 (MQ=60)

                        GCCAAACATGGCGGGCGGCATCCCCTTT‑CCCGCCCACGAGAGCGCCAACAGACCCAGCAAAGTGCTCACGAGATCAATGGAGCCTAAACAGGCG                                                                       >  SRR4302547.2969722/1‑94 (MQ=25)

                          CAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCT                                                                     >  SRR4302547.4130756/1‑94 (MQ=60)

                           AAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTA                                                                    <  SRR4302547.4477445/94‑1 (MQ=60)

                            AACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAT                                                                   <  SRR4302547.3106969/94‑1 (MQ=60)

                               ATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGC                                                                <  SRR4302547.971561/94‑1 (MQ=60)

                                TGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCG                                                               <  SRR4302547.3309311/94‑1 (MQ=60)

                                GGGCGGGCGTCACCCCTTTC‑CCGCCACACGAGAGCCCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCG                                                               <  SRR4302547.1492418/94‑1 (MQ=60)

                                  GCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAA                                                             <  SRR4302547.2866625/94‑1 (MQ=60)

                                  GCGGGCGCCACCCCTTTC‑CCGGCACACGAGAGCGCAAACAGCCCCAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAA                                                             <  SRR4302547.2625385/94‑1 (MQ=60)

                                   CGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAAT                                                            >  SRR4302547.2863368/1‑94 (MQ=60)

                                   CGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAAT                                                            >  SRR4302547.740049/1‑94 (MQ=60)

                                    GGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATA                                                           >  SRR4302547.3650197/1‑94 (MQ=60)

                                     GGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAA                                                          >  SRR4302547.6548218/1‑94 (MQ=60)

                                        cctctccctttccccggcacaggagagccccaaaagccccggaaggcCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302547.2535171/47‑1 (MQ=60)

                                        GTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302547.650152/94‑1 (MQ=60)

                                        GTCATCCCTTAC‑CCGGCCCACGAGACCGCCAACGGCCCCAGCAAGGCGCACACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATATTGG                                                       <  SRR4302547.2873043/94‑1 (MQ=55)

                                         TCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGT                                                      <  SRR4302547.6122545/94‑1 (MQ=60)

                                         TCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGT                                                      >  SRR4302547.1829527/1‑94 (MQ=60)

                                          CATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     >  SRR4302547.6497123/1‑94 (MQ=60)

                                          CATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     >  SRR4302547.5294256/1‑94 (MQ=60)

                                            TCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   >  SRR4302547.2064888/1‑94 (MQ=60)

                                             cccctcaccggccccacgagaccccaaacagcccaagcaggcccCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   <  SRR4302547.5869190/50‑1 (MQ=60)

                                             CCCTTTC‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  <  SRR4302547.4267045/94‑1 (MQ=60)

                                             CCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302547.102392/1‑94 (MQ=60)

                                             CCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302547.4024065/1‑94 (MQ=60)

                                              CCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAA                                                 >  SRR4302547.3663638/1‑94 (MQ=60)

                                               CTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                >  SRR4302547.1570288/1‑94 (MQ=60)

                                                TTTA‑CCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302547.1500945/94‑1 (MQ=60)

                                                 TTACCCGGCACACGAGAGCGCCAACAGACCAAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302547.6377282/94‑1 (MQ=60)

                                                  TA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGC                                             <  SRR4302547.6521367/94‑1 (MQ=60)

                                                   A‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCG                                            >  SRR4302547.954628/1‑94 (MQ=60)

                                                       GGCACACGAGCGCCCCAACAGACCCAGAAGGCCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCA                                         <  SRR4302547.1679958/94‑1 (MQ=60)

                                                       GGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCA                                         <  SRR4302547.3355638/94‑1 (MQ=60)

                                                         CACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATC                                       >  SRR4302547.2042772/1‑94 (MQ=60)

                                                         CACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATC                                       >  SRR4302547.4077838/1‑94 (MQ=60)

                                                          ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      >  SRR4302547.442540/1‑94 (MQ=60)

                                                          ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      >  SRR4302547.4278500/1‑94 (MQ=60)

                                                          ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      <  SRR4302547.5177379/94‑1 (MQ=60)

                                                          ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      <  SRR4302547.1688741/94‑1 (MQ=60)

                                                          ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCCAATAATGGTGCGAAACGCGCCATCT                                      <  SRR4302547.6540551/94‑1 (MQ=60)

                                                           CACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302547.4523152/94‑1 (MQ=60)

                                                           CACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     >  SRR4302547.6318067/1‑94 (MQ=60)

                                                             ggggaccccaAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCA                                   <  SRR4302547.4832989/84‑1 (MQ=60)

                                                             CGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCA                                   >  SRR4302547.1807547/1‑94 (MQ=60)

                                                              GAGAGCGCCAACTGTCCCAGCAAGGCGCTCACGACTTTACTGGAGCCTTACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGTCATCTGCAA                                  >  SRR4302547.6779780/1‑94 (MQ=60)

                                                                  gcccaaacagcccaAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCA                              <  SRR4302547.1324443/80‑1 (MQ=60)

                                                                  GCACCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCA                              <  SRR4302547.2382032/94‑1 (MQ=60)

                                                                   CGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAA                             <  SRR4302547.6744684/94‑1 (MQ=60)

                                                                    GCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            >  SRR4302547.1300332/1‑94 (MQ=60)

                                                                    GCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            >  SRR4302547.5711279/1‑94 (MQ=60)

                                                                    GCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            >  SRR4302547.853874/1‑94 (MQ=60)

                                                                      CAACAGACCGAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          <  SRR4302547.3711225/94‑1 (MQ=60)

                                                                      CAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          >  SRR4302547.2639426/1‑94 (MQ=60)

                                                                       aacagcccaaggaggcccCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302547.4682352/76‑1 (MQ=60)

                                                                       AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         >  SRR4302547.1139014/1‑94 (MQ=60)

                                                                       AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         >  SRR4302547.3497108/1‑94 (MQ=60)

                                                                        ACAGCCCCAGAAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302547.3820163/94‑1 (MQ=60)

                                                                        ACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302547.6280255/94‑1 (MQ=60)

                                                                         CAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       >  SRR4302547.5926908/1‑94 (MQ=60)

                                                                           GACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     >  SRR4302547.5189362/1‑94 (MQ=60)

                                                                            ACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATG                    >  SRR4302547.5716570/1‑94 (MQ=60)

                                                                             CCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   <  SRR4302547.4485542/94‑1 (MQ=60)

                                                                             CCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   <  SRR4302547.5509375/94‑1 (MQ=60)

                                                                              CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  >  SRR4302547.6498617/1‑94 (MQ=60)

                                                                              CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  >  SRR4302547.1518370/1‑94 (MQ=60)

                                                                              CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  >  SRR4302547.2593633/1‑94 (MQ=60)

                                                                                AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302547.3062586/1‑94 (MQ=60)

                                                                                AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                <  SRR4302547.1932334/94‑1 (MQ=60)

                                                                                  CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              >  SRR4302547.5529415/1‑94 (MQ=60)

                                                                                  CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              <  SRR4302547.1862815/94‑1 (MQ=60)

                                                                                  CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGAGCCATCTGCAAACCAATGTTTGATGTTAAAA              <  SRR4302547.5249226/94‑1 (MQ=60)

                                                                                   AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             >  SRR4302547.3979836/1‑94 (MQ=60)

                                                                                    AGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAATCCAATGTTTGATGTTAAAACC            <  SRR4302547.6532842/94‑1 (MQ=60)

                                                                                    AGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACC            >  SRR4302547.689982/1‑94 (MQ=60)

                                                                                    AGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACC            <  SRR4302547.845595/94‑1 (MQ=60)

                                                                                      GCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCG          <  SRR4302547.3125178/94‑1 (MQ=60)

                                                                                       CGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGC         >  SRR4302547.931040/1‑94 (MQ=60)

                                                                                        GCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCC        >  SRR4302547.6029835/1‑94 (MQ=60)

                                                                                        GCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCC        <  SRR4302547.3045217/94‑1 (MQ=60)

                                                                                         CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       >  SRR4302547.441254/1‑94 (MQ=60)

                                                                                              GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  SRR4302547.1126590/1‑94 (MQ=60)

                                                                                              CACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302547.5671546/94‑1 (MQ=60)

GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTA‑CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`