Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,197,191	T→C	Q31R (CAG→CGG)	glnE ←	GlnE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,197,191	0	T	C	98.0%	856.7 / 2.7	247	Q31R (CAG→CGG)	glnE	GlnE
Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (113/129); total (115/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 254 total aligned reads displayed.

GGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTC  >  NC_000913/3197101‑3197280

ggcggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgt                                                                                         >  1:2010549/1‑93 (MQ=255)

 gcggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCcgtg                                                                                        >  2:3655027/1‑93 (MQ=255)

  cggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTgc                                                                                       <  1:4351174/93‑1 (MQ=255)

    gTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTgcgc                                                                                     <  2:1190099/93‑1 (MQ=255)

    gTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTgcgc                                                                                     >  2:5208054/1‑93 (MQ=255)

     ttGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCt                                                                                    >  1:3140081/1‑93 (MQ=255)

     ttGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCt                                                                                    >  2:3986116/1‑93 (MQ=255)

     ttGCCTTTCCAGTTCCCTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCt                                                                                    <  1:3376062/93‑1 (MQ=255)

      tGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTa                                                                                   >  1:2956799/1‑93 (MQ=255)

       ggCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTaa                                                                                  >  2:5567247/1‑93 (MQ=255)

       ggCTTCCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTaa                                                                                  <  2:2556528/93‑1 (MQ=255)

        gCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAg                                                                                 >  2:3139285/1‑93 (MQ=255)

        gCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAg                                                                                 <  1:316371/93‑1 (MQ=255)

         cTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGt                                                                                >  1:5500883/1‑93 (MQ=255)

          tttCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTg                                                                               >  1:1965462/1‑93 (MQ=255)

           ttCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGa                                                                              <  2:3696305/93‑1 (MQ=255)

           ttCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGa                                                                              >  1:4408591/1‑93 (MQ=255)

           ttCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGa                                                                              <  2:5469095/93‑1 (MQ=255)

             ccAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGAtt                                                                            <  1:1168122/93‑1 (MQ=255)

             ccAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGAtt                                                                            <  2:6119172/93‑1 (MQ=255)

             ccAGTCCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGAtt                                                                            <  1:3069551/93‑1 (MQ=255)

              cAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTc                                                                           <  2:854968/93‑1 (MQ=255)

              cAGTCCCGCCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTc                                                                           <  2:2232241/93‑1 (MQ=255)

                gtcccGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCt                                                                         <  2:4368301/90‑1 (MQ=255)

                gTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCt                                                                         >  2:3935087/1‑93 (MQ=255)

                gTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCt                                                                         >  1:379472/1‑93 (MQ=255)

                gTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCt                                                                         <  1:4553038/93‑1 (MQ=255)

                gTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCt                                                                         >  2:2214779/1‑93 (MQ=255)

                   ccGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCgg                                                                      <  1:943229/93‑1 (MQ=255)

                        aGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTaaa                                                                 <  1:5113022/93‑1 (MQ=255)

                         gCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAg                                                                >  2:5579717/1‑93 (MQ=255)

                           cACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGc                                                              >  1:706635/1‑93 (MQ=255)

                            cctctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGct                                                             <  1:5206533/92‑1 (MQ=255)

                             ctctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctc                                                            <  1:3255383/93‑1 (MQ=255)

                             ctctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctc                                                            <  2:3244213/93‑1 (MQ=255)

                             ctctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctc                                                            >  1:3601351/1‑93 (MQ=255)

                              tctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGctct                                                           >  2:5262795/1‑93 (MQ=255)

                               ctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTg                                                          <  1:1090038/93‑1 (MQ=255)

                               ctGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTg                                                          <  1:1281061/93‑1 (MQ=255)

                                     gcgcAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGcc                                                    <  1:1007345/93‑1 (MQ=255)

                                        cAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCt                                                 >  2:1273166/1‑93 (MQ=255)

                                        cAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCt                                                 <  1:201183/93‑1 (MQ=255)

                                           tCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcac                                              >  2:1256839/1‑92 (MQ=255)

                                            cACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaac                                             <  1:2269451/93‑1 (MQ=255)

                                             aCGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaaca                                            >  2:2407675/1‑93 (MQ=255)

                                              cGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaacaa                                           >  1:6031642/1‑93 (MQ=255)

                                              cGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTcaacaa                                           <  2:3537360/93‑1 (MQ=255)

                                               gCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCaacaac                                          >  1:2688879/1‑93 (MQ=255)

                                                 tGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACgg                                        <  2:2036252/93‑1 (MQ=255)

                                                 tGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACgg                                        >  2:1167995/1‑93 (MQ=255)

                                                 tGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACgg                                        <  1:2382830/93‑1 (MQ=255)

                                                  gTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGt                                       >  1:5349582/1‑93 (MQ=255)

                                                   tCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTc                                      <  2:2741635/93‑1 (MQ=255)

                                                   tCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTc                                      <  2:1378764/93‑1 (MQ=255)

                                                    ccTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCt                                     >  2:3462146/1‑93 (MQ=255)

                                                    ccTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGACt                                     >  2:1088838/1‑93 (MQ=255)

                                                    ccTGCACAAAATCACTAAAAGTAAGCACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCt                                     >  2:3038609/1‑93 (MQ=255)

                                                      tGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGc                                   >  1:1352709/1‑93 (MQ=255)

                                                         acaAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAg                                <  1:413264/93‑1 (MQ=255)

                                                         acaAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAg                                >  1:1856504/1‑93 (MQ=255)

                                                          caAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGt                               >  2:2334821/1‑93 (MQ=255)

                                                           aaaaTCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTa                              >  2:5003056/1‑93 (MQ=255)

                                                            aaaTCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTAc                             <  2:1658750/93‑1 (MQ=255)

                                                            aaaTCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTAc                             <  1:3286071/93‑1 (MQ=255)

                                                             aaTCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTAct                            >  1:2637668/1‑93 (MQ=255)

                                                              aTCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActg                           >  1:1114347/1‑93 (MQ=255)

                                                                 aCTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTActgctg                        <  2:3830164/93‑1 (MQ=255)

                                                                  cTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGt                       >  2:2001815/1‑93 (MQ=255)

                                                                     aaaGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAAc                    >  2:448596/1‑93 (MQ=255)

                                                                     aaaGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAAc                    <  1:3394247/93‑1 (MQ=255)

                                                                      aagtaagtACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACg                   >  2:4735054/1‑93 (MQ=255)

                                                                      aagtaagtACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACg                   <  1:4778330/93‑1 (MQ=255)

                                                                       agtaagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACgg                  >  1:2503907/1‑93 (MQ=255)

                                                                        gtaagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGt                 >  2:5896508/1‑93 (MQ=255)

                                                                        gtaagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGt                 <  1:123307/93‑1 (MQ=255)

                                                                        ataagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGt                 <  2:4589459/92‑1 (MQ=255)

                                                                         taagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTg                >  1:5264533/1‑93 (MQ=255)

                                                                          aagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGa               <  1:4821860/93‑1 (MQ=255)

                                                                          aagtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGa               <  1:5544083/93‑1 (MQ=255)

                                                                          aagtaCTGACTTCGCCCGTGCGCTAAGTGACTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGa               <  1:871107/93‑1 (MQ=255)

                                                                           agtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGaa              <  1:4589862/93‑1 (MQ=255)

                                                                           agtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGaa              <  1:559077/93‑1 (MQ=255)

                                                                            gtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAag             <  1:5838568/93‑1 (MQ=255)

                                                                            gtaCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAag             >  2:6073730/1‑93 (MQ=255)

                                                                             taCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGATCTGGCAGCCGCTCAACACCGGTCTGCCAGTACTGCTGTCACGGTGAagg            >  2:5820041/1‑92 (MQ=255)

                                                                              aCTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagag           >  2:4569026/1‑93 (MQ=255)

                                                                               cTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagaga          >  2:284223/1‑93 (MQ=255)

                                                                               cTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagaga          >  2:2442372/1‑93 (MQ=255)

                                                                                tGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagagag         <  2:2575103/93‑1 (MQ=255)

                                                                                tGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagagag         <  1:608510/93‑1 (MQ=255)

                                                                                tGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAagagag         <  2:3087555/93‑1 (MQ=255)

                                                                                 gACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGc        <  2:1559083/93‑1 (MQ=255)

                                                                                  aCTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCg       >  2:5446799/1‑93 (MQ=255)

                                                                                  aCTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCg       >  1:5300142/1‑93 (MQ=255)

                                                                                   cTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCgg      >  2:4865816/1‑93 (MQ=255)

                                                                                   cTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCgg      >  2:3430815/1‑93 (MQ=255)

                                                                                    ttCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGc     >  2:4268056/1‑93 (MQ=255)

                                                                                    ttCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGc     <  1:5003927/93‑1 (MQ=255)

                                                                                    ttCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGc     >  1:4867526/1‑93 (MQ=255)

                                                                                      cGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCtt   >  1:1574802/1‑93 (MQ=255)

GGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTC  >  NC_000913/3197101‑3197280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAA  >  NC_000913/3197098‑3197284

TGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCC                                                                                               >  SRR4302532.4948928/1‑94 (MQ=60)

TGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCC                                                                                               >  SRR4302532.2787613/1‑94 (MQ=60)

  CGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGT                                                                                             >  SRR4302532.2077603/1‑94 (MQ=60)

    GCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGC                                                                                           >  SRR4302532.568780/1‑94 (MQ=60)

     CGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCG                                                                                          <  SRR4302532.4498300/94‑1 (MQ=60)

       GTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTTCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCT                                                                                        >  SRR4302532.5842115/1‑94 (MQ=60)

       GTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCT                                                                                        >  SRR4302532.3244292/1‑94 (MQ=60)

        TTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTA                                                                                       >  SRR4302532.3054776/1‑94 (MQ=60)

        TTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTA                                                                                       >  SRR4302532.11393/1‑94 (MQ=60)

        TTGCCTTTCCAGTTCCCTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTA                                                                                       <  SRR4302532.3488968/94‑1 (MQ=60)

           GCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGT                                                                                    <  SRR4302532.327344/94‑1 (MQ=60)

           GCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGT                                                                                    <  SRR4302532.3195149/94‑1 (MQ=60)

           GCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGT                                                                                    >  SRR4302532.5691689/1‑94 (MQ=60)

           GCTTTCCAGTTCCGTCAGCCACTATGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGT                                                                                    <  SRR4302532.3631450/94‑1 (MQ=60)

            CTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTG                                                                                   >  SRR4302532.2030745/1‑94 (MQ=60)

             TTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGA                                                                                  >  SRR4302532.4558757/1‑94 (MQ=60)

              TTCAAGTTCGGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTCCGCCCGTGCGCTAAGTGAT                                                                                 <  SRR4302532.5509583/94‑1 (MQ=60)

               TCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATT                                                                                >  SRR4302532.5255813/1‑94 (MQ=60)

                CCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTC                                                                               <  SRR4302532.1207145/94‑1 (MQ=60)

                CCAGTCCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTC                                                                               <  SRR4302532.3170980/94‑1 (MQ=60)

                CCACTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTC                                                                               <  SRR4302532.5581029/94‑1 (MQ=60)

                  AGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCT                                                                             >  SRR4302532.1390461/1‑94 (MQ=60)

                  AGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCT                                                                             >  SRR4302532.6130180/1‑94 (MQ=60)

                  AGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCT                                                                             <  SRR4302532.544500/94‑1 (MQ=60)

                  AGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCT                                                                             >  SRR4302532.392700/1‑94 (MQ=60)

                  AGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCT                                                                             <  SRR4302532.3390380/94‑1 (MQ=60)

                   GTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGGACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTC                                                                            >  SRR4302532.3503188/1‑94 (MQ=60)

                   GTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTC                                                                            <  SRR4302532.4708395/94‑1 (MQ=60)

                   GTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTC                                                                            >  SRR4302532.5448486/1‑94 (MQ=60)

                     TCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGG                                                                          <  SRR4302532.5742630/94‑1 (MQ=60)

                      CCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGC                                                                         <  SRR4302532.975532/94‑1 (MQ=60)

                           AGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAG                                                                    >  SRR4302532.325721/1‑94 (MQ=60)

                           AGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAG                                                                    <  SRR4302532.3214487/94‑1 (MQ=60)

                           AGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAG                                                                    <  SRR4302532.4909733/94‑1 (MQ=60)

                           AGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAG                                                                    <  SRR4302532.4959968/94‑1 (MQ=60)

                           AGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAG                                                                    <  SRR4302532.5288830/94‑1 (MQ=60)

                           AGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAG                                                                    <  SRR4302532.6112998/94‑1 (MQ=60)

                            GCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGG                                                                   <  SRR4302532.4570383/94‑1 (MQ=60)

                             CCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGC                                                                  >  SRR4302532.730789/1‑94 (MQ=60)

                              CACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCT                                                                 >  SRR4302532.114830/1‑94 (MQ=60)

                              CACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCT                                                                 <  SRR4302532.6333011/94‑1 (MQ=60)

                              CACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCT                                                                 <  SRR4302532.12890/94‑1 (MQ=60)

                               CCTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTC                                                                <  SRR4302532.5386071/94‑1 (MQ=60)

                               ACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTC                                                                >  SRR4302532.3721740/1‑94 (MQ=60)

                                CTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCT                                                               <  SRR4302532.5407615/94‑1 (MQ=60)

                                CTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCT                                                               <  SRR4302532.3363518/94‑1 (MQ=60)

                                 TCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTG                                                              <  SRR4302532.269978/94‑1 (MQ=60)

                                 TCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTG                                                              <  SRR4302532.493046/94‑1 (MQ=60)

                                  CTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGG                                                             <  SRR4302532.1126515/94‑1 (MQ=60)

                                  CTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGG                                                             <  SRR4302532.1323916/94‑1 (MQ=60)

                                    GGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGCAAGTACTGACTTCGCCCGCGCGCTAAGTGATTCCTCGGATAAAGGCTCTGGCA                                                           >  SRR4302532.3484960/1‑94 (MQ=60)

                                     GATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAG                                                          <  SRR4302532.3578681/94‑1 (MQ=60)

                                       TGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCC                                                        <  SRR4302532.2533946/94‑1 (MQ=60)

                                        GCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCG                                                       <  SRR4302532.1041608/94‑1 (MQ=60)

                                         CGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGC                                                      <  SRR4302532.1252574/94‑1 (MQ=60)

                                           CAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTC                                                    <  SRR4302532.208049/94‑1 (MQ=60)

                                           CAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTC                                                    >  SRR4302532.676502/1‑94 (MQ=60)

                                           CAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTC                                                    <  SRR4302532.208050/94‑1 (MQ=60)

                                              TCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAAC                                                 >  SRR4302532.4335136/1‑94 (MQ=60)

                                               CACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACA                                                <  SRR4302532.2344770/94‑1 (MQ=60)

                                                ACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAA                                               >  SRR4302532.6239956/1‑94 (MQ=60)

                                                 CGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAAC                                              >  SRR4302532.2779194/1‑94 (MQ=60)

                                                 CGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAAC                                              <  SRR4302532.5285407/94‑1 (MQ=60)

                                                 CGCTGTCCTGCACAAAATCACTAAAAGAAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAAC                                              >  SRR4302532.1186204/1‑94 (MQ=60)

                                                  GCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGTCGCCTCTCAACAACG                                             >  SRR4302532.1403498/1‑94 (MQ=60)

                                                  GCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACG                                             <  SRR4302532.3731742/94‑1 (MQ=60)

                                                  GCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACG                                             <  SRR4302532.3280275/94‑1 (MQ=60)

                                                    TGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGT                                           <  SRR4302532.2462212/94‑1 (MQ=60)

                                                    TGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGT                                           <  SRR4302532.5476039/94‑1 (MQ=60)

                                                    TGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGT                                           >  SRR4302532.5535156/1‑94 (MQ=60)

                                                    TGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGT                                           <  SRR4302532.122186/94‑1 (MQ=60)

                                                      TCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCT                                         <  SRR4302532.2392332/94‑1 (MQ=60)

                                                        CTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGC                                       >  SRR4302532.1397955/1‑94 (MQ=60)

                                                         TGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCC                                      >  SRR4302532.1362711/1‑94 (MQ=60)

                                                           CACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAG                                    >  SRR4302532.1918056/1‑94 (MQ=60)

                                                            ACAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGT                                   <  SRR4302532.427652/94‑1 (MQ=60)

                                                             CAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTA                                  <  SRR4302532.284375/94‑1 (MQ=60)

                                                             CAAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTA                                  >  SRR4302532.2612016/1‑94 (MQ=60)

                                                              AAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTAC                                 >  SRR4302532.5045711/1‑94 (MQ=60)

                                                              AAAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTAC                                 >  SRR4302532.2067607/1‑94 (MQ=60)

                                                               AAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACT                                <  SRR4302532.3395379/94‑1 (MQ=60)

                                                               AAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACT                                <  SRR4302532.4455290/94‑1 (MQ=60)

                                                               AAATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACT                                >  SRR4302532.2726079/1‑94 (MQ=60)

                                                                AATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTG                               <  SRR4302532.4052047/94‑1 (MQ=60)

                                                                AATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTG                               >  SRR4302532.1151586/1‑94 (MQ=60)

                                                                 ATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGC                              <  SRR4302532.3300583/94‑1 (MQ=60)

                                                                 ATCACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGC                              >  SRR4302532.929702/1‑94 (MQ=60)

                                                                  tctctacatGTAAGTACTGACTTCGCCCGTGCGCTAGGTGATTACTCGGCTAAAGGCTCTGGCAGCCGCTCCACAACGGTCTGCCAGTACTGCT                             <  SRR4302532.5689053/85‑1 (MQ=60)

                                                                  TCACTAAAAGTAAGTACTGACTTCACCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCT                             >  SRR4302532.5488838/1‑94 (MQ=60)

                                                                   CACTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTG                            >  SRR4302532.3486524/1‑94 (MQ=60)

                                                                   CACTAAAAGTAAATACTGACTTCGCCCGTGCGCTAAGTGATTCCCCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTG                            <  SRR4302532.1968290/94‑1 (MQ=60)

                                                                    CCTAAAAGTAAGTCCTGACTTCGCGCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGT                           <  SRR4302532.4720000/94‑1 (MQ=60)

                                                                     CTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTA                          <  SRR4302532.2268481/94‑1 (MQ=60)

                                                                     CTAAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTA                          <  SRR4302532.5768781/94‑1 (MQ=60)

                                                                       AAAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAAC                        <  SRR4302532.5693617/94‑1 (MQ=60)

                                                                        AAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACG                       <  SRR4302532.1783750/94‑1 (MQ=60)

                                                                        AAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACG                       <  SRR4302532.3507797/94‑1 (MQ=60)

                                                                        AAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACG                       <  SRR4302532.1062919/94‑1 (MQ=60)

                                                                        AAAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACG                       <  SRR4302532.307597/94‑1 (MQ=60)

                                                                         AAGTAAGTACTGCCTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGG                      <  SRR4302532.952083/94‑1 (MQ=60)

                                                                         AAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGG                      <  SRR4302532.5028227/94‑1 (MQ=60)

                                                                         AAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGG                      <  SRR4302532.4942885/94‑1 (MQ=60)

                                                                         AAGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGG                      >  SRR4302532.2587415/1‑94 (MQ=60)

                                                                          AGTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGT                     <  SRR4302532.2749304/94‑1 (MQ=60)

                                                                           gtaaggcctcACTTCGCCCGTCCGCTAAGTGATTCCTCGGATAAAGCCTCTGGCAACCGCTCAACAACGGTCTGCCAGGACTGCTTTAACGGTG                    <  SRR4302532.377113/84‑1 (MQ=58)

                                                                           GTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTG                    <  SRR4302532.2359041/94‑1 (MQ=60)

                                                                           GTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTG                    >  SRR4302532.5446094/1‑94 (MQ=60)

                                                                           GTAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTG                    <  SRR4302532.127495/94‑1 (MQ=60)

                                                                            TAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGA                   >  SRR4302532.502064/1‑94 (MQ=60)

                                                                             AAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAA                  <  SRR4302532.4987856/94‑1 (MQ=60)

                                                                             AAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAA                  <  SRR4302532.5736508/94‑1 (MQ=60)

                                                                             AAGTACTGACTTCGCCCGTGCGCTAAGTGACTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAA                  <  SRR4302532.900935/94‑1 (MQ=60)

                                                                              AGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAG                 <  SRR4302532.4746345/94‑1 (MQ=60)

                                                                              AGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAG                 <  SRR4302532.578573/94‑1 (MQ=60)

                                                                               GTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGA                <  SRR4302532.6041213/94‑1 (MQ=60)

                                                                               GTACTGACTTCGCCCGTGCCCTAAGTGATTCCCCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGA                <  SRR4302532.5322232/94‑1 (MQ=60)

                                                                                TACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAG               >  SRR4302532.6272206/1‑94 (MQ=60)

                                                                                 ACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGA              <  SRR4302532.4525883/94‑1 (MQ=60)

                                                                                 ACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGA              <  SRR4302532.2056390/94‑1 (MQ=60)

                                                                                   TGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGC            >  SRR4302532.2206250/1‑94 (MQ=60)

                                                                                   TGACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGC            <  SRR4302532.629734/94‑1 (MQ=60)

                                                                                    GACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCG           >  SRR4302532.5483275/1‑94 (MQ=60)

                                                                                     GCTTCGCCCGTGCTCTAAGTGCTCCCTCGGCTAAAGGCTCTGCCAGCCGCTCACCAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCCG          <  SRR4302532.3986614/94‑1 (MQ=60)

                                                                                     ACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGG          >  SRR4302532.3805169/1‑94 (MQ=60)

                                                                                     ACTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGG          <  SRR4302532.814334/94‑1 (MQ=60)

                                                                                      CTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGC         >  SRR4302532.4659019/1‑94 (MQ=60)

                                                                                      CTTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGC         >  SRR4302532.5034900/1‑94 (MQ=60)

                                                                                       TTCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCT        <  SRR4302532.5175452/94‑1 (MQ=60)

                                                                                        TCGCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTT       >  SRR4302532.1628118/1‑94 (MQ=60)

                                                                                          GCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCA     <  SRR4302532.2973737/94‑1 (MQ=60)

                                                                                          GCCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCA     <  SRR4302532.166429/94‑1 (MQ=60)

                                                                                           CCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCAT    >  SRR4302532.1133764/1‑94 (MQ=60)

                                                                                           CCCGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCAT    >  SRR4302532.2046593/1‑94 (MQ=60)

                                                                                             CGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAA  <  SRR4302532.6156658/94‑1 (MQ=60)

                                                                                             CGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAA  >  SRR4302532.613087/1‑94 (MQ=60)

                                                                                             CGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAA  <  SRR4302532.2845155/94‑1 (MQ=60)

TGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAA  >  NC_000913/3197098‑3197284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`