Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,203,865	C→T	L11L (TTG→TTA)	ygiP ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,203,865	0	C	T	88.9%	873.2 / 70.3	280	L11L (TTG→TTA)	ygiP	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base C (17/14); new base T (111/138); total (128/152)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

Only 100 of 285 total aligned reads displayed.

TGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT  >  NC_000913/3203773‑3203956

tGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGc                                                                                             >  2:4309167/1‑93 (MQ=255)

    tCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAgc                                                                                         >  1:4806689/1‑92 (MQ=255)

      cGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCt                                                                                       <  1:1113205/93‑1 (MQ=255)

      cGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCt                                                                                       >  1:1665775/1‑93 (MQ=255)

       gCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCtt                                                                                      >  1:1576797/1‑93 (MQ=255)

       gCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCtt                                                                                      <  2:6431438/93‑1 (MQ=255)

       gCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCtt                                                                                      <  2:4546538/93‑1 (MQ=255)

         ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  2:2159667/93‑1 (MQ=255)

         ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  2:3277681/93‑1 (MQ=255)

         ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  2:493515/93‑1 (MQ=255)

         ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  2:4959842/93‑1 (MQ=255)

         ttGGTGACAAATGCAGGCGGCTGCCCAAGCGTCGCCGCTGCGTCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTg                                                                                    <  1:510726/93‑1 (MQ=255)

          tGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTgg                                                                                   >  2:502709/1‑93 (MQ=255)

          tGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTgg                                                                                   >  1:1312783/1‑93 (MQ=255)

           ggTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGc                                                                                  <  2:6148726/93‑1 (MQ=255)

           ggTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGc                                                                                  >  1:3354826/1‑93 (MQ=255)

           ggTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGCGAACGATTTCTACCAGTACCTGTAAGTCTTTGGc                                                                                  >  2:785470/1‑93 (MQ=255)

            gTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCt                                                                                 <  2:1251396/93‑1 (MQ=255)

             tGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTa                                                                                >  2:5873837/1‑93 (MQ=255)

             ggACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTa                                                                                <  2:1885178/92‑1 (MQ=255)

              gACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTaa                                                                               >  1:3698513/1‑93 (MQ=255)

               aCAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTaaa                                                                              <  1:999023/93‑1 (MQ=255)

               aCAAAAGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTaaa                                                                              <  1:1556758/93‑1 (MQ=255)

                cAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAg                                                                             <  1:2808262/93‑1 (MQ=255)

                 aaaTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAgg                                                                            <  2:4803062/93‑1 (MQ=255)

                   aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGcc                                                                          >  2:6602869/1‑93 (MQ=255)

                   aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGcc                                                                          >  1:4947990/1‑93 (MQ=255)

                   aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGcc                                                                          >  1:2516766/1‑93 (MQ=255)

                     gCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAg                                                                        <  2:1145755/93‑1 (MQ=255)

                     gCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAg                                                                        <  2:5550471/93‑1 (MQ=255)

                      cAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGc                                                                       >  2:1050569/1‑93 (MQ=255)

                       aGGCGTCTGCCCAAGCGTCGCCGCTGCGGCTCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCt                                                                      <  2:1410974/93‑1 (MQ=255)

                       aGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCt                                                                      >  1:3195167/1‑93 (MQ=255)

                       aGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCt                                                                      >  2:3903743/1‑93 (MQ=255)

                          cGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTAtt                                                                   >  2:2496815/1‑93 (MQ=255)

                          cGTCTCCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTAtt                                                                   <  1:5226423/93‑1 (MQ=255)

                           gTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTc                                                                  >  1:4102400/1‑93 (MQ=255)

                            tCTGCCCGAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCa                                                                 <  1:3965458/93‑1 (MQ=255)

                               gCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCa                                                              <  1:2987176/93‑1 (MQ=255)

                                 ccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGTCCAGCTATTCAGCATc                                                            >  1:642713/1‑93 (MQ=255)

                                 ccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATc                                                            >  2:957113/1‑93 (MQ=255)

                                 ccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATc                                                            >  1:4206085/1‑93 (MQ=255)

                                     gCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTa                                                        >  2:5355475/1‑93 (MQ=255)

                                         cgccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTa                                                    >  1:2723279/1‑93 (MQ=255)

                                         cgccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTa                                                    <  2:4735300/93‑1 (MQ=255)

                                         cgccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTa                                                    <  2:2260658/93‑1 (MQ=255)

                                          gccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTAt                                                   >  1:5180447/1‑93 (MQ=255)

                                           ccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATc                                                  >  2:3007838/1‑93 (MQ=255)

                                           ccgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATc                                                  <  1:6294928/93‑1 (MQ=255)

                                            cgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATcc                                                 <  1:1234876/93‑1 (MQ=255)

                                            cgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATcc                                                 >  2:3544012/1‑93 (MQ=255)

                                            cgcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATcc                                                 <  2:4121449/93‑1 (MQ=255)

                                             gcTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCg                                                >  1:2067752/1‑93 (MQ=255)

                                              cTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGGCAGCTAGTCAGCATCGGTCATTATCGGc                                               >  2:1587965/1‑93 (MQ=255)

                                              cTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGc                                               <  2:1708474/93‑1 (MQ=255)

                                               tGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCa                                              <  1:4438319/93‑1 (MQ=255)

                                               tGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCa                                              <  1:253191/93‑1 (MQ=255)

                                                gcggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAg                                             <  1:6206770/93‑1 (MQ=255)

                                                gcggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAg                                             <  1:2017155/93‑1 (MQ=255)

                                                 cggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            >  1:3078978/1‑93 (MQ=255)

                                                 cggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            <  2:1719096/93‑1 (MQ=255)

                                                 cggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            >  2:2811228/1‑93 (MQ=255)

                                                 cggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            <  1:105653/93‑1 (MQ=255)

                                                  gtcgcTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGtt                                           <  1:5996463/91‑1 (MQ=255)

                                                  ggcgCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGtt                                           <  2:3671447/93‑1 (MQ=255)

                                                    cgcTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTtgt                                         <  2:89014/93‑1 (MQ=255)

                                                     gcTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTtgtg                                        >  2:6002925/1‑93 (MQ=255)

                                                      cTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGa                                       <  2:5726083/93‑1 (MQ=255)

                                                      cTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGa                                       >  2:3719180/1‑93 (MQ=255)

                                                       tAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGAt                                      <  1:3886830/93‑1 (MQ=255)

                                                         aaaCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATaa                                    >  2:2394407/1‑93 (MQ=255)

                                                          aaCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAg                                   >  2:2265041/1‑93 (MQ=255)

                                                           aCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAgc                                  >  2:3211273/1‑93 (MQ=255)

                                                            cTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAgcg                                 >  2:2168617/1‑93 (MQ=255)

                                                                 gCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAgtg                            <  2:5277754/93‑1 (MQ=255)

                                                                  cTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAgtgt                           >  1:729639/1‑93 (MQ=255)

                                                                   tgtgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTa                          >  1:868939/1‑93 (MQ=255)

                                                                   tgtgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTa                          <  1:5693463/93‑1 (MQ=255)

                                                                    gtgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAt                         <  2:6322120/93‑1 (MQ=255)

                                                                    gtgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAt                         <  1:3437381/93‑1 (MQ=255)

                                                                     tgAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAtt                        >  1:2196972/1‑93 (MQ=255)

                                                                     tgAACGATTTCCACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAtt                        <  2:2360342/93‑1 (MQ=255)

                                                                      gAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       <  2:1394131/93‑1 (MQ=255)

                                                                      gAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       <  2:3099992/93‑1 (MQ=255)

                                                                      gAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       >  2:2687784/1‑93 (MQ=255)

                                                                         cGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGc                    <  1:2321115/93‑1 (MQ=255)

                                                                          gATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCa                   >  2:152529/1‑93 (MQ=255)

                                                                           aTTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCaa                  >  1:5382940/1‑93 (MQ=255)

                                                                               cTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACa              <  1:3487207/93‑1 (MQ=255)

                                                                                 aCCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACAtt            >  2:2094775/1‑93 (MQ=255)

                                                                                  ccAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTg           >  2:4833128/1‑93 (MQ=255)

                                                                                   cAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGc          <  1:6364835/93‑1 (MQ=255)

                                                                                   cAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGc          >  1:2050715/1‑93 (MQ=255)

                                                                                   cAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGc          <  1:3258264/93‑1 (MQ=255)

                                                                                    aGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGcc         <  1:1363840/93‑1 (MQ=255)

                                                                                    aGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGcc         >  2:3347650/1‑93 (MQ=255)

                                                                                       aCCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCAtt      <  2:6484673/93‑1 (MQ=255)

                                                                                       aCCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCAtt      >  2:2233226/1‑93 (MQ=255)

                                                                                         cTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTcc    <  2:644907/93‑1 (MQ=255)

                                                                                          tGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTccc   <  1:5435864/93‑1 (MQ=255)

TGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT  >  NC_000913/3203773‑3203956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGC  >  NC_000913/3203772‑3203958

TTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGT                                                                                               >  SRR4302547.781541/1‑94 (MQ=60)

 TGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTA                                                                                              >  SRR4302547.1632992/1‑94 (MQ=60)

 TGGAGCCGCTTGGTGCCAGATGCAGGCGTCTCCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTCCCTGAA                                                                                              <  SRR4302547.1502434/94‑1 (MQ=60)

   GATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTAACTGTAAG                                                                                            >  SRR4302547.2841502/1‑94 (MQ=60)

   GATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGCACCTGTAAG                                                                                            <  SRR4302547.6632410/94‑1 (MQ=60)

    ATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGT                                                                                           >  SRR4302547.2260323/1‑94 (MQ=60)

     TCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTC                                                                                          >  SRR4302547.2784258/1‑94 (MQ=60)

     TCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTC                                                                                          >  SRR4302547.4166431/1‑94 (MQ=60)

      CCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCT                                                                                         >  SRR4302547.1698613/1‑94 (MQ=60)

       CGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTT                                                                                        <  SRR4302547.1953516/94‑1 (MQ=60)

       CGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTT                                                                                        >  SRR4302547.342614/1‑94 (MQ=60)

       CGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTT                                                                                        <  SRR4302547.1135624/94‑1 (MQ=60)

       CGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTT                                                                                        >  SRR4302547.1608177/1‑94 (MQ=60)

        GCTTGGTGACAAATCCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAGCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTT                                                                                       <  SRR4302547.4374888/94‑1 (MQ=60)

         CTTGGTGACAAATTCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTG                                                                                      <  SRR4302547.6459320/94‑1 (MQ=60)

         CTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAGCGATTTCTACCAGTACCTGTAAGTCTTTG                                                                                      <  SRR4302547.3540553/94‑1 (MQ=60)

          TTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGG                                                                                     <  SRR4302547.6338062/94‑1 (MQ=60)

          TTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGG                                                                                     >  SRR4302547.1339182/1‑94 (MQ=60)

          TTGGTGACAAATGCAGGCGGCTGCCCAAGCGTCGCCGCTGCGTCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGG                                                                                     <  SRR4302547.519997/94‑1 (MQ=60)

           TGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGC                                                                                    >  SRR4302547.3422867/1‑94 (MQ=60)

             GTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTA                                                                                  >  SRR4302547.1215067/1‑94 (MQ=60)

             GTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTA                                                                                  <  SRR4302547.2978795/94‑1 (MQ=60)

              TGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAA                                                                                 >  SRR4302547.3774002/1‑94 (MQ=60)

               GACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAA                                                                                >  SRR4302547.3418130/1‑94 (MQ=60)

               GACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAA                                                                                <  SRR4302547.4958969/94‑1 (MQ=60)

                ACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAG                                                                               <  SRR4302547.3312813/94‑1 (MQ=60)

                ACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAG                                                                               <  SRR4302547.1018503/94‑1 (MQ=60)

                ACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAG                                                                               <  SRR4302547.931688/94‑1 (MQ=60)

                ACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAG                                                                               <  SRR4302547.2320044/94‑1 (MQ=60)

                ACAAAAGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAG                                                                               <  SRR4302547.1587730/94‑1 (MQ=60)

                 CAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGG                                                                              <  SRR4302547.2753638/94‑1 (MQ=60)

                 CAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGG                                                                              >  SRR4302547.2920237/1‑94 (MQ=60)

                 CAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGG                                                                              <  SRR4302547.2864460/94‑1 (MQ=60)

                   AATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCC                                                                            >  SRR4302547.5050458/1‑94 (MQ=60)

                   AATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCC                                                                            >  SRR4302547.1301540/1‑94 (MQ=60)

                   AATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCC                                                                            <  SRR4302547.1348057/94‑1 (MQ=60)

                   AATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCC                                                                            >  SRR4302547.2567643/1‑94 (MQ=60)

                     TGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAG                                                                          >  SRR4302547.1629732/1‑94 (MQ=60)

                       CAGGCGTCTGCCCAAGCGTCGCTGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCT                                                                        <  SRR4302547.426658/94‑1 (MQ=60)

                       CAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCT                                                                        >  SRR4302547.3258834/1‑94 (MQ=60)

                       CAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCT                                                                        >  SRR4302547.5301907/1‑94 (MQ=60)

                        AGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTA                                                                       <  SRR4302547.1306216/94‑1 (MQ=60)

                         GGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTAT                                                                      >  SRR4302547.5980218/1‑94 (MQ=60)

                           CGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTC                                                                    >  SRR4302547.4185813/1‑94 (MQ=60)

                           CGTCTCCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTC                                                                    <  SRR4302547.5334000/94‑1 (MQ=60)

                             TCTGCCCGAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAG                                                                  <  SRR4302547.4046108/94‑1 (MQ=60)

                             TCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAG                                                                  <  SRR4302547.483736/94‑1 (MQ=60)

                               TGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCA                                                                <  SRR4302547.1606290/94‑1 (MQ=60)

                                GCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCAT                                                               <  SRR4302547.3046610/94‑1 (MQ=60)

                                GCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCAT                                                               <  SRR4302547.5336106/94‑1 (MQ=60)

                                 CCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGTCCAGCTATTCAGCATC                                                              >  SRR4302547.654566/1‑94 (MQ=60)

                                 CCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATC                                                              >  SRR4302547.4291912/1‑94 (MQ=60)

                                    AAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTT                                                           >  SRR4302547.1227373/1‑94 (MQ=60)

                                     AGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTA                                                          <  SRR4302547.2570439/94‑1 (MQ=60)

                                      GCGTCGCCGCTGCGGCGCTAAACCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAA                                                         <  SRR4302547.1357902/94‑1 (MQ=60)

                                      GCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAA                                                         <  SRR4302547.3752833/94‑1 (MQ=60)

                                       CGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAAT                                                        <  SRR4302547.3522617/94‑1 (MQ=60)

                                       CGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAAT                                                        <  SRR4302547.221940/94‑1 (MQ=60)

                                        GTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATT                                                       >  SRR4302547.894650/1‑94 (MQ=60)

                                         TCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTA                                                      >  SRR4302547.2777962/1‑94 (MQ=60)

                                          CGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTAT                                                     >  SRR4302547.5287065/1‑94 (MQ=60)

                                          CGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTAT                                                     <  SRR4302547.3826768/94‑1 (MQ=60)

                                          CGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTAT                                                     >  SRR4302547.5906193/1‑94 (MQ=60)

                                           GCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATC                                                    <  SRR4302547.5283670/94‑1 (MQ=60)

                                            CCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCC                                                   <  SRR4302547.6425785/94‑1 (MQ=60)

                                             CGCTGCGGCGCTAAATCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCG                                                  <  SRR4302547.3817201/94‑1 (MQ=60)

                                             CGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCG                                                  >  SRR4302547.5427349/1‑94 (MQ=60)

                                             CGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCG                                                  >  SRR4302547.2109016/1‑94 (MQ=60)

                                             CGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCG                                                  <  SRR4302547.1259789/94‑1 (MQ=60)

                                                TGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAG                                               >  SRR4302547.3289499/1‑94 (MQ=60)

                                                TGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAG                                               <  SRR4302547.257928/94‑1 (MQ=60)

                                                TGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAG                                               >  SRR4302547.2443047/1‑94 (MQ=60)

                                                TGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAG                                               <  SRR4302547.4530085/94‑1 (MQ=60)

                                                TGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAG                                               <  SRR4302547.2089734/94‑1 (MQ=60)

                                                 GCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGT                                              <  SRR4302547.6335635/94‑1 (MQ=60)

                                                 GCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGT                                              >  SRR4302547.3140125/1‑94 (MQ=60)

                                                 GCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGT                                              <  SRR4302547.2057284/94‑1 (MQ=60)

                                                 GCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGT                                              <  SRR4302547.5645436/94‑1 (MQ=60)

                                                  CGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTT                                             <  SRR4302547.3059330/94‑1 (MQ=60)

                                                  CGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTT                                             <  SRR4302547.879183/94‑1 (MQ=60)

                                                  CGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTT                                             <  SRR4302547.107576/94‑1 (MQ=60)

                                                   GTCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTG                                            <  SRR4302547.6120903/94‑1 (MQ=60)

                                                   GGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTG                                            <  SRR4302547.5540664/94‑1 (MQ=60)

                                                   GGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTG                                            <  SRR4302547.4688860/94‑1 (MQ=60)

                                                   GGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTG                                            >  SRR4302547.6395415/1‑94 (MQ=60)

                                                     CGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTG                                          >  SRR4302547.3215936/1‑94 (MQ=60)

                                                      GCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGA                                         >  SRR4302547.6302510/1‑94 (MQ=60)

                                                        TAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATA                                       <  SRR4302547.3965894/94‑1 (MQ=60)

                                                           ATCTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTGTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGC                                    <  SRR4302547.4029916/94‑1 (MQ=60)

                                                            ACTCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCG                                   <  SRR4302547.510890/94‑1 (MQ=60)

                                                            ACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCG                                   <  SRR4302547.719788/94‑1 (MQ=60)

                                                             CTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGC                                  >  SRR4302547.3170331/1‑94 (MQ=60)

                                                              TCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCA                                 >  SRR4302547.5508715/1‑94 (MQ=60)

                                                              TCCCGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCA                                 >  SRR4302547.1555899/1‑94 (MQ=60)

                                                                 CGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTG                              <  SRR4302547.1597164/94‑1 (MQ=60)

                                                                 CGCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTG                              <  SRR4302547.5214091/94‑1 (MQ=60)

                                                                 CGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTG                              <  SRR4302547.1118890/94‑1 (MQ=60)

                                                                  GCTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGT                             >  SRR4302547.743251/1‑94 (MQ=60)

                                                                   CTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTA                            >  SRR4302547.885578/1‑94 (MQ=60)

                                                                   CTGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTA                            <  SRR4302547.2157646/94‑1 (MQ=60)

                                                                    TGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAT                           >  SRR4302547.4279435/1‑94 (MQ=60)

                                                                    TGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAT                           <  SRR4302547.5811055/94‑1 (MQ=60)

                                                                    TGTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAT                           <  SRR4302547.3080625/94‑1 (MQ=60)

                                                                     GTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATT                          <  SRR4302547.762334/94‑1 (MQ=60)

                                                                     GTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATT                          <  SRR4302547.3507468/94‑1 (MQ=60)

                                                                     GTGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATT                          >  SRR4302547.2241099/1‑94 (MQ=60)

                                                                      TGAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTT                         <  SRR4302547.2727685/94‑1 (MQ=60)

                                                                       gaacatcttctaccacttctTGCAAATCTTCGGCTACAGGCCAGCTATGCAGCATCGTTAATTATCCGCAGTTGTGAGAAGCGCTGTGTATTTC                        <  SRR4302547.801095/74‑1 (MQ=52)

                                                                       GAACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTC                        >  SRR4302547.2061715/1‑94 (MQ=60)

                                                                        AACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCG                       <  SRR4302547.2096039/94‑1 (MQ=60)

                                                                        AACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCG                       >  SRR4302547.44861/1‑94 (MQ=60)

                                                                        AACGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCG                       <  SRR4302547.2513053/94‑1 (MQ=60)

                                                                          CGATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCA                     <  SRR4302547.2368157/94‑1 (MQ=60)

                                                                           GATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAA                    >  SRR4302547.5494084/1‑94 (MQ=60)

                                                                           GATTTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAA                    >  SRR4302547.5732626/1‑94 (MQ=60)

                                                                              TTCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAAC                 <  SRR4302547.5013864/94‑1 (MQ=60)

                                                                               TCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACA                <  SRR4302547.6507838/94‑1 (MQ=60)

                                                                               TCTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACA                <  SRR4302547.6507839/94‑1 (MQ=60)

                                                                                CTACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACAT               <  SRR4302547.3558358/94‑1 (MQ=60)

                                                                                  ACCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTG             <  SRR4302547.3469248/94‑1 (MQ=60)

                                                                                   CCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGC            >  SRR4302547.2091617/1‑94 (MQ=60)

                                                                                   CCAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGC            >  SRR4302547.1493110/1‑94 (MQ=60)

                                                                                    CAGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCC           <  SRR4302547.6497620/94‑1 (MQ=60)

                                                                                    CAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCC           <  SRR4302547.3728680/94‑1 (MQ=60)

                                                                                    CAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCC           <  SRR4302547.3323381/94‑1 (MQ=60)

                                                                                     AGTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA          <  SRR4302547.1391202/94‑1 (MQ=60)

                                                                                      GTACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCAT         >  SRR4302547.2094881/1‑94 (MQ=60)

                                                                                       TACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATT        >  SRR4302547.6235322/1‑94 (MQ=60)

                                                                                        ACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTC       >  SRR4302547.5082447/1‑94 (MQ=60)

                                                                                        ACCTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTC       <  SRR4302547.4005838/94‑1 (MQ=60)

                                                                                          CTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCC     >  SRR4302547.4646015/1‑94 (MQ=60)

                                                                                          CTGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCC     >  SRR4302547.4180168/1‑94 (MQ=60)

                                                                                          CTGGAAGTCTTTGGCTAAAGGGCAGCTATTCAGCATCGTTAATTATCCGCAGTTGGGATAAGCGCAGTGTATTTCGCAAAACAATGCCAatcca     <  SRR4302547.1770914/94‑6 (MQ=60)

                                                                                           TGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT    >  SRR4302547.3749009/1‑94 (MQ=60)

                                                                                           TGTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT    <  SRR4302547.2403982/94‑1 (MQ=60)

                                                                                           TGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT    <  SRR4302547.5548217/94‑1 (MQ=60)

                                                                                            GTAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTG   >  SRR4302547.4667032/1‑94 (MQ=60)

                                                                                             CAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGC  >  SRR4302547.2133238/1‑94 (MQ=60)

TTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGC  >  NC_000913/3203772‑3203958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`