Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,298,112	T→C	G96G (GGA→GGG)	yhbS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,298,112	0	T	C	99.2%	817.5 / ‑4.1	239	G96G (GGA→GGG)	yhbS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (117/119); total (119/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 248 total aligned reads displayed.

GCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGTCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTC  >  NC_000913/3298023‑3298201

 ccgccAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCtt                                                                                       <  1:525754/93‑1 (MQ=255)

  cgccAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTc                                                                                      >  1:2096652/1‑93 (MQ=255)

  cgccAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTc                                                                                      <  1:16191/93‑1 (MQ=255)

   gccAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCa                                                                                     >  2:12697/1‑93 (MQ=255)

   gccAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCa                                                                                     >  2:3821412/1‑93 (MQ=255)

    ccAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCAt                                                                                    <  1:4098766/93‑1 (MQ=255)

     cAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATa                                                                                   >  2:45616/1‑93 (MQ=255)

     cAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCCAGCCCTTCATa                                                                                   >  1:312736/1‑93 (MQ=255)

       gTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATCGa                                                                                 >  1:1479722/1‑93 (MQ=255)

       gTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGa                                                                                 <  1:648010/93‑1 (MQ=255)

        ttCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGAc                                                                                <  1:1257362/93‑1 (MQ=255)

          cAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCa                                                                              <  2:2665827/93‑1 (MQ=255)

            aaGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGt                                                                            <  2:2870635/93‑1 (MQ=255)

            aaGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGt                                                                            <  2:2615470/93‑1 (MQ=255)

             aGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGtt                                                                           <  1:5927862/93‑1 (MQ=255)

             aGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGtt                                                                           >  2:4475647/1‑93 (MQ=255)

              gCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTg                                                                          <  1:4152245/93‑1 (MQ=255)

              gCCGAAACGACTGTACAGCACCGGAACGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAAACGAGCCCTTCATAGACCAGTTg                                                                          <  1:5649703/93‑1 (MQ=255)

               ccGAAACGACTTTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTgg                                                                         <  1:4980219/93‑1 (MQ=255)

                cGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGCACAGTTGGc                                                                        >  2:2154856/1‑93 (MQ=255)

                cGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGc                                                                        >  1:5846707/1‑93 (MQ=255)

                 gAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCg                                                                       >  1:4261086/1‑93 (MQ=255)

                    aCGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGc                                                                    >  1:5189348/1‑93 (MQ=255)

                      gACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCa                                                                  <  1:2821157/93‑1 (MQ=255)

                      gACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCa                                                                  <  2:64978/93‑1 (MQ=255)

                      gACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCa                                                                  >  2:2813122/1‑93 (MQ=255)

                           tACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGcccc                                                             <  1:917139/93‑1 (MQ=255)

                            aCAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCt                                                            >  2:5180466/1‑93 (MQ=255)

                            aCAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCt                                                            >  2:2935944/1‑93 (MQ=255)

                              aGCGCCGGATCG‑CCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGGCCTTCATAAAACAGTTGGGGTGCCAAGCCCCGTc                                                         >  2:4461112/1‑93 (MQ=255)

                                cgcCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTcc                                                        <  2:5863563/93‑1 (MQ=255)

                                cgcCGGATCGCCCACCGTCA‑CACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCa                                                       <  1:1900165/93‑1 (MQ=255)

                                 gcCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCa                                                       >  2:777217/1‑93 (MQ=255)

                                  ccGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCAc                                                      <  1:713196/93‑1 (MQ=255)

                                  ccGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCAc                                                      >  2:4059887/1‑93 (MQ=255)

                                  ccGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCAc                                                      >  1:3898712/1‑93 (MQ=255)

                                    ggATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACgg                                                    <  2:212461/93‑1 (MQ=255)

                                    ggATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACgg                                                    >  1:3082037/1‑93 (MQ=255)

                                    ggATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGACCCTGTCCACgg                                                    >  1:2522718/1‑93 (MQ=255)

                                      aTCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTa                                                  <  2:3637521/93‑1 (MQ=255)

                                      aTCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTa                                                  <  1:4004824/93‑1 (MQ=255)

                                        cGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGCCCACGGCAtt                                                >  2:5725436/1‑93 (MQ=255)

                                         gCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTAttt                                               <  2:5994963/93‑1 (MQ=255)

                                           ccAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTAttttt                                             <  1:542919/93‑1 (MQ=255)

                                            cAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTtc                                            >  1:289221/1‑93 (MQ=255)

                                            cAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTtc                                            >  2:4643163/1‑93 (MQ=255)

                                             aGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTtca                                           >  2:5434737/1‑93 (MQ=255)

                                             aGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTtca                                           >  2:5711857/1‑93 (MQ=255)

                                              gCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTtcat                                          >  2:3351367/1‑93 (MQ=255)

                                              gCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTtcat                                          >  2:5101055/1‑93 (MQ=255)

                                               cGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTtcatc                                         <  1:2634672/93‑1 (MQ=255)

                                                gTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCTCCTGTCCACGGTATGTTtcatca                                        >  1:4278880/1‑93 (MQ=255)

                                                 tCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCaa                                       >  2:5240399/1‑93 (MQ=255)

                                                 tCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCaa                                       <  2:5286058/93‑1 (MQ=255)

                                                   accacTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAAcc                                     <  1:4892164/93‑1 (MQ=255)

                                                   accacTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAAcc                                     >  2:3478696/1‑93 (MQ=255)

                                                   accacTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAAcc                                     <  1:1139922/93‑1 (MQ=255)

                                                     cacTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGc                                   >  1:1711465/1‑93 (MQ=255)

                                                       cTGCGGAATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGCCTTTTTCATCAACCGCCa                                 >  1:2562156/1‑93 (MQ=255)

                                                         gCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAAc                               >  2:1689050/1‑93 (MQ=255)

                                                          cGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACg                              <  1:3925708/93‑1 (MQ=255)

                                                          cGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACg                              >  2:3126740/1‑93 (MQ=255)

                                                          cGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGCATTTTTCATCAAACGCCAACg                              >  1:4522558/1‑93 (MQ=255)

                                                           ggCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACgg                             <  2:1989195/93‑1 (MQ=255)

                                                            gCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGt                            >  1:878000/1‑93 (MQ=255)

                                                             cATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGtc                           >  2:4930808/1‑92 (MQ=255)

                                                             cATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTg                           <  1:1213092/93‑1 (MQ=255)

                                                              aTAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGc                          >  2:3214636/1‑93 (MQ=255)

                                                               tAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGcc                         <  1:3713470/93‑1 (MQ=255)

                                                               tAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGcc                         <  1:54542/93‑1 (MQ=255)

                                                                   cGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGc                     <  2:826665/93‑1 (MQ=255)

                                                                   cGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGc                     <  2:2379901/93‑1 (MQ=255)

                                                                    gAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGcc                    >  2:3541395/1‑93 (MQ=255)

                                                                    gAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCAAGGTATTTTTCATCAACCGCCAACGGTGCCATGcc                    >  2:3996355/1‑93 (MQ=255)

                                                                     aaCTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACTGCCAACGGTGCCATGCCg                   >  1:1957557/1‑93 (MQ=255)

                                                                     aaCTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCg                   >  2:4805487/1‑93 (MQ=255)

                                                                      aCTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGa                  <  1:3261507/93‑1 (MQ=255)

                                                                      aCTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGGGCCATGCCgc                  >  1:209892/1‑92 (MQ=255)

                                                                       cTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGAc                 >  1:4718646/1‑93 (MQ=255)

                                                                        tCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGAcc                >  1:2148863/1‑93 (MQ=255)

                                                                        tCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGAcc                >  1:2529323/1‑93 (MQ=255)

                                                                           ttAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCAt             <  2:5360244/93‑1 (MQ=255)

                                                                            tAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCAtt            >  1:1387122/1‑93 (MQ=255)

                                                                            tAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGGATTTTTCATCAAACGCCAACGGTGCCATGCAGACCCAtt            >  1:1574080/1‑93 (MQ=255)

                                                                             aaGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTg           >  2:2452165/1‑93 (MQ=255)

                                                                             aaGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTg           >  1:977919/1‑93 (MQ=255)

                                                                              aGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGGCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGc          <  2:4176806/93‑1 (MQ=255)

                                                                               gCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCa         >  1:3983986/1‑93 (MQ=255)

                                                                               gCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCa         <  1:5728326/93‑1 (MQ=255)

                                                                                 gAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAgg       <  1:3397675/93‑1 (MQ=255)

                                                                                 gAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAgg       <  1:4796042/93‑1 (MQ=255)

                                                                                 gAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAgg       <  2:1143862/93‑1 (MQ=255)

                                                                                 gAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAgg       <  2:1905809/93‑1 (MQ=255)

                                                                                   aTCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTc     <  2:1495990/93‑1 (MQ=255)

                                                                                   aTCAAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTc     <  1:1817009/93‑1 (MQ=255)

                                                                                     cGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCtt   <  1:2176825/93‑1 (MQ=255)

                                                                                     cGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCtt   <  2:2699922/93‑1 (MQ=255)

                                                                                      gAGGCCTTCATAGACCAGTTGGCGTGCCATCCCCTGTCCATGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTc  <  2:6064779/93‑1 (MQ=255)

                                                                                      gAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTc  <  1:4466713/93‑1 (MQ=255)

                                                                                      gAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTc  <  2:4226489/93‑1 (MQ=255)

GCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGTCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTC  >  NC_000913/3298023‑3298201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCGCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGTCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTCGCCC  >  NC_000913/3298020‑3298205

TGCGCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCC                                                                                              <  SRR4302532.786728/94‑1 (MQ=60)

  CGCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCT                                                                                            >  SRR4302532.1027754/1‑94 (MQ=60)

  CGCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCT                                                                                            >  SRR4302532.3342090/1‑94 (MQ=60)

  CGCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCT                                                                                            >  SRR4302532.2185538/1‑94 (MQ=60)

   GCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTT                                                                                           <  SRR4302532.4419849/94‑1 (MQ=60)

    CCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTC                                                                                          <  SRR4302532.544099/94‑1 (MQ=60)

    CCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTC                                                                                          >  SRR4302532.2166585/1‑94 (MQ=60)

     CGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCA                                                                                         <  SRR4302532.16847/94‑1 (MQ=60)

     CGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAAGCGAGCCCTTCA                                                                                         >  SRR4302532.1732583/1‑94 (MQ=60)

      GCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTCAGAGAATCGAGCGCTTCAT                                                                                        >  SRR4302532.956126/1‑94 (MQ=60)

      GCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCAT                                                                                        >  SRR4302532.2947061/1‑94 (MQ=60)

       CCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATA                                                                                       >  SRR4302532.511420/1‑94 (MQ=60)

       CCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATA                                                                                       <  SRR4302532.4236354/94‑1 (MQ=60)

       CCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATA                                                                                       <  SRR4302532.4010322/94‑1 (MQ=60)

       CCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATA                                                                                       <  SRR4302532.3807290/94‑1 (MQ=60)

       CCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCCAGCCCTTCATA                                                                                       >  SRR4302532.323583/1‑94 (MQ=60)

       CCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGAGCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATA                                                                                       >  SRR4302532.4108848/1‑94 (MQ=60)

        CAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAG                                                                                      <  SRR4302532.5052080/94‑1 (MQ=60)

         AGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATCGA                                                                                     >  SRR4302532.1529590/1‑94 (MQ=60)

         AGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGACCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGACCTTCATAGA                                                                                     >  SRR4302532.1255406/1‑94 (MQ=60)

          GTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGAC                                                                                    <  SRR4302532.670498/94‑1 (MQ=60)

           TTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACC                                                                                   <  SRR4302532.1299430/94‑1 (MQ=60)

              AAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGCCCATT                                                                                >  SRR4302532.1998283/1‑94 (MQ=60)

                AGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTG                                                                              <  SRR4302532.6133089/94‑1 (MQ=60)

                AGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTG                                                                              <  SRR4302532.5014046/94‑1 (MQ=60)

                AGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTG                                                                              <  SRR4302532.1209442/94‑1 (MQ=60)

                AGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTG                                                                              <  SRR4302532.2144858/94‑1 (MQ=60)

                 GCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGG                                                                             <  SRR4302532.4291545/94‑1 (MQ=60)

                 GCCGAAACGACTGTACAGCACCGGAACGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAAACGAGCCCTTCATAGACCAGTTGG                                                                             <  SRR4302532.5846272/94‑1 (MQ=60)

                  CCGAAACGACTTTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGC                                                                            <  SRR4302532.5150943/94‑1 (MQ=60)

                  CCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGC                                                                            >  SRR4302532.6049594/1‑94 (MQ=60)

                   CGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCG                                                                           >  SRR4302532.4404446/1‑94 (MQ=60)

                    GAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGT                                                                          <  SRR4302532.4716900/94‑1 (MQ=60)

                     AAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTG                                                                         <  SRR4302532.4241045/94‑1 (MQ=60)

                      AACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGC                                                                        >  SRR4302532.5368194/1‑94 (MQ=60)

                      AACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGATGGCGTGC                                                                        >  SRR4302532.5661275/1‑94 (MQ=60)

                         GACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAG                                                                     <  SRR4302532.2915565/94‑1 (MQ=60)

                         GACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAG                                                                     >  SRR4302532.4352928/1‑94 (MQ=60)

                          ACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGC                                                                    >  SRR4302532.1880260/1‑94 (MQ=60)

                          ACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGC                                                                    >  SRR4302532.3180437/1‑94 (MQ=60)

                           CTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCC                                                                   >  SRR4302532.3382805/1‑94 (MQ=60)

                           CTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCC                                                                   >  SRR4302532.6050943/1‑94 (MQ=60)

                            TGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCC                                                                  >  SRR4302532.4902360/1‑94 (MQ=60)

                             GTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCC                                                                 <  SRR4302532.1532858/94‑1 (MQ=60)

                              TACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCT                                                                >  SRR4302532.788167/1‑94 (MQ=60)

                              TACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCT                                                                <  SRR4302532.948519/94‑1 (MQ=60)

                              TACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCT                                                                <  SRR4302532.5943225/94‑1 (MQ=60)

                                CAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGT                                                              <  SRR4302532.4858876/94‑1 (MQ=60)

                                CAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGT                                                              >  SRR4302532.4593334/1‑94 (MQ=60)

                                   CGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCA                                                           >  SRR4302532.4068996/1‑94 (MQ=60)

                                   CGCCGGATCGCCCACCGTCA‑CACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCAC                                                          <  SRR4302532.1963177/94‑1 (MQ=60)

                                    GCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCAC                                                          >  SRR4302532.4030215/1‑94 (MQ=60)

                                     CCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACG                                                         <  SRR4302532.737562/94‑1 (MQ=60)

                                      CGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGG                                                        <  SRR4302532.2520403/94‑1 (MQ=60)

                                      CGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGG                                                        >  SRR4302532.3183974/1‑94 (MQ=60)

                                      CGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGACCCTGTCCACGG                                                        >  SRR4302532.2606907/1‑94 (MQ=60)

                                       GGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGT                                                       >  SRR4302532.2415158/1‑94 (MQ=60)

                                       GGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGT                                                       >  SRR4302532.5321941/1‑94 (MQ=60)

                                        GATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTA                                                      <  SRR4302532.3226059/94‑1 (MQ=60)

                                        GATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTA                                                      >  SRR4302532.1653558/1‑94 (MQ=60)

                                         ATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTAT                                                     <  SRR4302532.4139763/94‑1 (MQ=60)

                                         ATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCCGTACACGGGAT                                                     >  SRR4302532.1372882/1‑94 (MQ=60)

                                            GCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTT                                                  <  SRR4302532.698464/94‑1 (MQ=60)

                                             CCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTT                                                 <  SRR4302532.2606938/94‑1 (MQ=60)

                                              CCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTC                                                >  SRR4302532.299195/1‑94 (MQ=60)

                                              CCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTC                                                <  SRR4302532.561831/94‑1 (MQ=60)

                                              CCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTC                                                >  SRR4302532.4119082/1‑94 (MQ=60)

                                              CCAGCGTCACCACTGAGGAATAGCCGAACTCATTAAGCGAATAGAGCCATTCATAGATCAGATGGCGTGACAGAACCTATCCACGGTATTTTTC                                                >  SRR4302532.4119083/1‑94 (MQ=32)

                                                AGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCAT                                              >  SRR4302532.183952/1‑94 (MQ=60)

                                                  CGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCTCCTGTCCACGGTATGTTTCATCA                                            >  SRR4302532.4422985/1‑94 (MQ=60)

                                                  CGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCA                                            <  SRR4302532.2722945/94‑1 (MQ=60)

                                                  CGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGACAGCCCCTGTCCACGGTATTTTTCATCA                                            >  SRR4302532.847653/1‑94 (MQ=60)

                                                   GTCACCACTGAGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGAGAAGTTGGCGTGCCAGCCGCTGTCCACGGTATTTTTCATCAA                                           >  SRR4302532.4370100/1‑94 (MQ=60)

                                                     CACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACC                                         >  SRR4302532.5878414/1‑94 (MQ=60)

                                                     CACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACC                                         <  SRR4302532.2895619/94‑1 (MQ=60)

                                                      ACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCG                                        <  SRR4302532.1319542/94‑1 (MQ=60)

                                                      ACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCG                                        <  SRR4302532.1177929/94‑1 (MQ=60)

                                                      ACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCG                                        <  SRR4302532.4162320/94‑1 (MQ=60)

                                                      ACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCG                                        <  SRR4302532.351723/94‑1 (MQ=60)

                                                      ACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCG                                        <  SRR4302532.5060281/94‑1 (MQ=60)

                                                       CCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGC                                       >  SRR4302532.1769158/1‑94 (MQ=60)

                                                        CACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCC                                      >  SRR4302532.1462766/1‑94 (MQ=60)

                                                        CACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCC                                      <  SRR4302532.5989128/94‑1 (MQ=60)

                                                         ACTGCGGAATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGCCTTTTTCATCAACCGCCA                                     >  SRR4302532.2647642/1‑94 (MQ=60)

                                                          CTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAC                                    >  SRR4302532.1563894/1‑94 (MQ=60)

                                                          CTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAA                                    >  SRR4302532.1738148/1‑94 (MQ=60)

                                                          CTGCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAA                                    >  SRR4302532.194497/1‑94 (MQ=60)

                                                            GCGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGCATTTTTCATCAAACGCCAACG                                  >  SRR4302532.4676927/1‑94 (MQ=60)

                                                             CGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGG                                 >  SRR4302532.917986/1‑94 (MQ=60)

                                                             CGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGG                                 >  SRR4302532.5549438/1‑94 (MQ=60)

                                                             CGGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGG                                 <  SRR4302532.4057964/94‑1 (MQ=60)

                                                              GGCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGT                                >  SRR4302532.908031/1‑94 (MQ=60)

                                                               GCATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTG                               <  SRR4302532.6160044/94‑1 (MQ=60)

                                                                CATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGC                              <  SRR4302532.1253693/94‑1 (MQ=60)

                                                                 ATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCC                             >  SRR4302532.4676281/1‑94 (MQ=60)

                                                                 ATAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCC                             >  SRR4302532.5633707/1‑94 (MQ=60)

                                                                  TAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCA                            <  SRR4302532.56487/94‑1 (MQ=60)

                                                                  TAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCA                            <  SRR4302532.4352440/94‑1 (MQ=60)

                                                                  TAGCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCA                            <  SRR4302532.3838114/94‑1 (MQ=60)

                                                                    GCCGAGCTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCTATG                          <  SRR4302532.640854/94‑1 (MQ=60)

                                                                    GCCGAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATG                          <  SRR4302532.2640538/94‑1 (MQ=60)

                                                                       GAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACTGCCAACGGTGCCATGCCG                       >  SRR4302532.2022583/1‑94 (MQ=60)

                                                                       GAACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCG                       >  SRR4302532.3088390/1‑94 (MQ=60)

                                                                        AACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGC                      >  SRR4302532.5788342/1‑94 (MQ=60)

                                                                        AACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGA                      >  SRR4302532.217049/1‑94 (MQ=60)

                                                                        AACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGGGCCATGCCGC                      >  SRR4302532.217050/1‑94 (MQ=60)

                                                                         ACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGAC                     >  SRR4302532.4879643/1‑94 (MQ=60)

                                                                         ACTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGAC                     <  SRR4302532.3369945/94‑1 (MQ=60)

                                                                          CTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACC                    >  SRR4302532.2613720/1‑94 (MQ=60)

                                                                          CTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACC                    >  SRR4302532.2220669/1‑94 (MQ=60)

                                                                          CTCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGCATTTTTCATCAACCGCCAACGGTGCCATGCCGACC                    >  SRR4302532.2768500/1‑94 (MQ=60)

                                                                           TCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCC                   >  SRR4302532.2653925/1‑94 (MQ=60)

                                                                           TCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCC                   >  SRR4302532.5341035/1‑94 (MQ=60)

                                                                           TCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCC                   >  SRR4302532.4048692/1‑94 (MQ=60)

                                                                           TCATTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACAGTGCCATGCCGACCC                   >  SRR4302532.5605089/1‑94 (MQ=60)

                                                                              TTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATT                >  SRR4302532.1433604/1‑94 (MQ=60)

                                                                              TTAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGGATTTTTCATCAAACGCCAACGGTGCCATGCAGACCCATT                >  SRR4302532.1627368/1‑94 (MQ=60)

                                                                               TAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTG               >  SRR4302532.1011293/1‑94 (MQ=60)

                                                                               TAAGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTG               >  SRR4302532.2617125/1‑94 (MQ=60)

                                                                                 AGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCA             <  SRR4302532.5411292/94‑1 (MQ=60)

                                                                                 AGCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCA             >  SRR4302532.4118190/1‑94 (MQ=60)

                                                                                  GCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAG            <  SRR4302532.5927830/94‑1 (MQ=60)

                                                                                  GCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAG            <  SRR4302532.3240611/94‑1 (MQ=60)

                                                                                  GCGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAG            <  SRR4302532.2246827/94‑1 (MQ=60)

                                                                                   CGAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGG           >  SRR4302532.4193995/1‑94 (MQ=60)

                                                                                    GAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGT          <  SRR4302532.4961213/94‑1 (MQ=60)

                                                                                    GAATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGT          <  SRR4302532.3511415/94‑1 (MQ=60)

                                                                                      ATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCT        >  SRR4302532.2176862/1‑94 (MQ=60)

                                                                                      ATCGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCT        <  SRR4302532.2135509/94‑1 (MQ=60)

                                                                                      ATCAAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCT        <  SRR4302532.1877578/94‑1 (MQ=60)

                                                                                        CGAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTC      <  SRR4302532.2249527/94‑1 (MQ=60)

                                                                                         GAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTCG     <  SRR4302532.4618995/94‑1 (MQ=60)

                                                                                         GAGCCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTCG     <  SRR4302532.4618994/94‑1 (MQ=60)

                                                                                            CCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTCGCCC  >  SRR4302532.1874599/1‑94 (MQ=60)

                                                                                            CCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTCGCCA  <  SRR4302532.6018210/94‑1 (MQ=60)

TGCGCCGCCAGTTCAAAGCCGAAACGACTGTACAGCGCCGGATCGCCCAGCGTCACCACTGCGGCATAGCCGAACTCATTAAGCGAATCGAGTCCTTCATAGACCAGTTGGCGTGCCAGCCCCTGTCCACGGTATTTTTCATCAACCGCCAACGGTGCCATGCCGACCCATTGCAGGTCTTCGCCC  >  NC_000913/3298020‑3298205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`