Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,510,121 +G intergenic (‑358/+151) yhfZ ← / ← trpS hypothetical protein/tryptophan tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,510,1201.G96.0% 708.8 / 17.2 202intergenic (‑357/+152)yhfZ/trpShypothetical protein/tryptophan tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (7/1);  new base G (120/74);  total (127/75)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG  >  NC_000913/3510028‑3510209
                                                                                              |                                                                                         
aaGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑cccc                                                                                            <  1:3325264/93‑1 (MQ=255)
 aGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCC‑g                                                                                          >  2:351935/1‑93 (MQ=255)
 aGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCC‑g                                                                                          >  2:1474470/1‑93 (MQ=255)
    tAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCC‑Ggtt                                                                                       >  2:3654180/1‑93 (MQ=255)
     aaGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCC‑Ggttt                                                                                      >  1:3275555/1‑92 (MQ=255)
      aGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCC‑Ggtttc                                                                                     <  1:5031341/93‑3 (MQ=255)
      aGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCC‑Ggtttc                                                                                     >  1:346288/1‑91 (MQ=255)
         cgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCaga                                                                                   <  1:3090971/93‑1 (MQ=255)
          gcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCagag                                                                                  <  1:1468798/93‑1 (MQ=255)
          gcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCagag                                                                                  <  2:3305831/93‑1 (MQ=255)
               aGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGtgatg                                                                             >  1:3372686/1‑93 (MQ=255)
                gCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGtgatga                                                                            >  2:715939/1‑93 (MQ=255)
                gCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGtgatga                                                                            <  2:1251052/93‑1 (MQ=255)
                gCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGtgatga                                                                            >  1:4872240/1‑93 (MQ=255)
                  gagaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATa                                                                          <  2:1045332/93‑1 (MQ=255)
                   agaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGATT‑CCCCGGTTTCAGAGTGATGATaa                                                                         >  2:2394514/1‑93 (MQ=255)
                    gaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATaaa                                                                        >  1:586266/1‑93 (MQ=255)
                     aTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGTTaaaa                                                                       <  2:4618556/93‑1 (MQ=255)
                      tgtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAg                                                                      >  1:3121360/1‑93 (MQ=255)
                       gtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGc                                                                     <  2:6015809/93‑1 (MQ=255)
                          gAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCaaa                                                                  >  1:5162941/1‑93 (MQ=255)
                          gAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCaaa                                                                  >  1:365515/1‑93 (MQ=255)
                           aaaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAATTGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCaaaa                                                                 >  2:3136155/1‑93 (MQ=255)
                           aaaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCaaaa                                                                 <  2:3359099/93‑1 (MQ=255)
                            aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                >  2:5002756/1‑93 (MQ=255)
                             aCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAAtt                                                               <  2:2895237/93‑1 (MQ=255)
                               ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGc                                                             <  2:5092354/93‑1 (MQ=255)
                               ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGc                                                             >  1:2025500/1‑93 (MQ=255)
                                gCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGcc                                                            <  2:3881497/93‑1 (MQ=255)
                                   gggCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGa                                                         <  2:4040490/92‑1 (MQ=255)
                                   aGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGa                                                         <  1:4230599/93‑1 (MQ=255)
                                   aGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGa                                                         >  2:5304497/1‑93 (MQ=255)
                                   aGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGa                                                         <  1:2793612/93‑1 (MQ=255)
                                    ggCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAt                                                        >  2:5443668/1‑93 (MQ=255)
                                    ggCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAt                                                        <  2:4434542/93‑1 (MQ=255)
                                       aCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcg                                                     >  1:708985/1‑93 (MQ=255)
                                       aCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcg                                                     >  2:5898575/1‑93 (MQ=255)
                                        cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc                                                    >  1:5415574/1‑93 (MQ=255)
                                        cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc                                                    >  1:128133/1‑93 (MQ=255)
                                         ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTGCCCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                   >  2:3408402/1‑93 (MQ=255)
                                          tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                  >  2:516384/1‑93 (MQ=255)
                                           cacaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc                                                 >  2:1356576/1‑93 (MQ=255)
                                                tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTa                                            <  2:4956942/93‑1 (MQ=255)
                                                tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTa                                            <  2:476490/93‑1 (MQ=255)
                                                  tttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATc                                          >  2:5830468/1‑93 (MQ=255)
                                                   ttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCa                                         >  2:254812/1‑93 (MQ=255)
                                                     cGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAgg                                       >  2:5591095/1‑93 (MQ=255)
                                                     cGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAgg                                       <  2:561596/93‑1 (MQ=255)
                                                      ggATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGc                                      >  1:2488634/1‑93 (MQ=255)
                                                      ggATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGc                                      <  1:6042108/93‑1 (MQ=255)
                                                       gATTATTGCAAATGAGGATAAAAAACCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                     <  2:119834/93‑1 (MQ=255)
                                                         ttattGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTa                                   >  2:1733333/1‑93 (MQ=255)
                                                          tattGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAc                                  >  2:2992739/1‑93 (MQ=255)
                                                            ttGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAt                                <  2:2719405/93‑1 (MQ=255)
                                                            ttGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCATACAt                                >  2:1546501/1‑93 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               <  2:1705158/93‑1 (MQ=255)
                                                               cAAATGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTc                             <  2:1426699/93‑1 (MQ=255)
                                                                aaaTGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTcc                            >  1:616592/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           <  2:4901315/93‑1 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  2:2124833/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  1:2949502/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  1:3960102/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           <  1:3837072/93‑1 (MQ=255)
                                                                   tGAGGATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTg                         <  1:2854796/93‑1 (MQ=255)
                                                                      ggATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCaa                      <  2:5718615/93‑1 (MQ=255)
                                                                       gATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                     >  1:5203175/1‑93 (MQ=255)
                                                                       gATAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                     <  2:5271821/93‑1 (MQ=255)
                                                                        aTAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAata                    <  1:4057218/93‑1 (MQ=255)
                                                                         tAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAatat                   >  2:2070289/1‑93 (MQ=255)
                                                                         tAAAAAAACCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAatat                   >  1:5307761/1‑93 (MQ=255)
                                                                          aaaaaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  <  2:3946140/93‑1 (MQ=255)
                                                                          aaaaaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  <  2:1528754/93‑1 (MQ=255)
                                                                          aaaaaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  >  1:2161170/1‑93 (MQ=255)
                                                                           aaaaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgt                 >  1:2686405/1‑93 (MQ=255)
                                                                            aaaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                >  1:235064/1‑93 (MQ=255)
                                                                            aaaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                >  2:3975571/1‑93 (MQ=255)
                                                                            aaaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                <  1:4416681/93‑1 (MQ=255)
                                                                             aaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGc               <  1:1066660/93‑1 (MQ=255)
                                                                             aaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGc               <  2:5011230/93‑1 (MQ=255)
                                                                             aaaaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGc               >  2:3018918/1‑93 (MQ=255)
                                                                              aaaCCGGGTTT‑CCCCGGGTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              >  2:1686054/1‑93 (MQ=255)
                                                                               aaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAt             >  1:1020927/1‑93 (MQ=255)
                                                                               aaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAt             >  1:3028572/1‑93 (MQ=255)
                                                                               aaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAt             <  1:37908/93‑1 (MQ=255)
                                                                               aaCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCGTATCAGGCCTACATTTCCTTGCAATATGTGCAt             >  2:5930783/1‑93 (MQ=255)
                                                                                aCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtt            >  1:467937/1‑93 (MQ=255)
                                                                                aCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtt            >  1:2572438/1‑93 (MQ=255)
                                                                                aCCGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtt            <  1:2092829/93‑1 (MQ=255)
                                                                                 ccGGGTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTa           >  1:804204/1‑93 (MQ=255)
                                                                                   gggTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACt         >  1:2258343/1‑93 (MQ=255)
                                                                                     gTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACttt       >  1:561648/1‑93 (MQ=255)
                                                                                     gTTT‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACttt       <  2:5634508/93‑1 (MQ=255)
                                                                                      ttt‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTg      <  2:2563592/93‑1 (MQ=255)
                                                                                       ttccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGt     <  2:1795158/90‑1 (MQ=255)
                                                                                       ttccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGt     <  2:2643919/90‑1 (MQ=255)
                                                                                        tccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa    <  1:3563104/91‑1 (MQ=255)
                                                                                        t‑CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa    >  2:4311815/1‑93 (MQ=255)
                                                                                          cccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAgg  >  1:462894/1‑93 (MQ=255)
                                                                                          cccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAgg  >  2:6117581/1‑93 (MQ=255)
                                                                                          cccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAgg  >  2:3517842/1‑93 (MQ=255)
                                                                                              |                                                                                         
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTT‑CCCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG  >  NC_000913/3510028‑3510209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG  >  NC_000913/3510028‑3510212
                                                                                              |                                                                                           
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑G                                                                                             <  SRR4302532.3436147/94‑1 (MQ=60)
 AGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GG                                                                                            >  SRR4302532.5748892/1‑94 (MQ=60)
 AGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GG                                                                                            <  SRR4302532.4454488/94‑1 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACGTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCTGGTTTCCCC‑GGTT                                                                                          <  SRR4302532.4729930/94‑1 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GGTT                                                                                          >  SRR4302532.5734926/1‑94 (MQ=60)
    TAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTT                                                                                          >  SRR4302532.3384484/1‑94 (MQ=60)
     AAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTC                                                                                         >  SRR4302532.358273/1‑94 (MQ=60)
      AGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCA                                                                                        <  SRR4302532.5203907/94‑1 (MQ=60)
       GGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAG                                                                                       >  SRR4302532.3415758/1‑94 (MQ=60)
       GGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAG                                                                                       >  SRR4302532.361936/1‑94 (MQ=60)
       AGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAG                                                                                       <  SRR4302532.5461087/94‑1 (MQ=60)
         CGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAG                                                                                     <  SRR4302532.3193401/94‑1 (MQ=60)
          GCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGT                                                                                    <  SRR4302532.1518277/94‑1 (MQ=60)
              CAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATG                                                                                >  SRR4302532.5546532/1‑94 (MQ=60)
              CAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATG                                                                                >  SRR4302532.3485465/1‑94 (MQ=60)
               AGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGA                                                                               >  SRR4302532.3155326/1‑94 (MQ=60)
               AGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGA                                                                               >  SRR4302532.5039737/1‑94 (MQ=60)
                GCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGAT                                                                              <  SRR4302532.2987390/94‑1 (MQ=60)
                   AGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAA                                                                           >  SRR4302532.606723/1‑94 (MQ=60)
                   AGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAA                                                                           >  SRR4302532.2241929/1‑94 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAG                                                                         >  SRR4302532.3224930/1‑94 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAG                                                                         <  SRR4302532.3463758/94‑1 (MQ=60)
                      TGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGC                                                                        >  SRR4302532.3833279/1‑94 (MQ=60)
                        TGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAA                                                                      >  SRR4302532.2952816/1‑94 (MQ=60)
                         GGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAA                                                                     >  SRR4302532.378201/1‑94 (MQ=60)
                         GGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAA                                                                     >  SRR4302532.719909/1‑94 (MQ=60)
                         GGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAA                                                                     >  SRR4302532.5340636/1‑94 (MQ=60)
                         GGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAA                                                                     <  SRR4302532.2841940/94‑1 (MQ=60)
                             ACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTG                                                                 <  SRR4302532.6346067/94‑1 (MQ=60)
                              CGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGC                                                                >  SRR4302532.2093025/1‑94 (MQ=60)
                                  GAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGA                                                            >  SRR4302532.415320/1‑94 (MQ=60)
                                  GAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGA                                                            >  SRR4302532.4225240/1‑94 (MQ=60)
                                  GAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGA                                                            >  SRR4302532.4934516/1‑94 (MQ=60)
                                   AGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAT                                                           <  SRR4302532.2887269/94‑1 (MQ=60)
                                   AGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAT                                                           >  SRR4302532.2660750/1‑94 (MQ=60)
                                   AGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAT                                                           <  SRR4302532.2542479/94‑1 (MQ=60)
                                   AGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAT                                                           <  SRR4302532.4372614/94‑1 (MQ=60)
                                   AGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAT                                                           <  SRR4302532.5368033/94‑1 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                        >  SRR4302532.733209/1‑94 (MQ=60)
                                       ACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGC                                                       >  SRR4302532.2935083/1‑94 (MQ=60)
                                       ACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGC                                                       >  SRR4302532.5603241/1‑94 (MQ=60)
                                       ACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGC                                                       >  SRR4302532.132467/1‑94 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                     >  SRR4302532.3286248/1‑94 (MQ=60)
                                          TCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAC                                                    <  SRR4302532.2134274/94‑1 (MQ=60)
                                             CATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCT                                                 >  SRR4302532.3206279/1‑94 (MQ=60)
                                               TTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTA                                               >  SRR4302532.943054/1‑94 (MQ=60)
                                                TTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAT                                              <  SRR4302532.1712195/94‑1 (MQ=60)
                                                     CGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGC                                         >  SRR4302532.2571568/1‑94 (MQ=60)
                                                      GGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCC                                        <  SRR4302532.3231739/94‑1 (MQ=60)
                                                      GGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCC                                        <  SRR4302532.6250739/94‑1 (MQ=60)
                                                       GATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                       >  SRR4302532.2753130/1‑94 (MQ=60)
                                                       GATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                       <  SRR4302532.2240035/94‑1 (MQ=60)
                                                        ATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTA                                      >  SRR4302532.4794876/1‑94 (MQ=60)
                                                        ATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTA                                      >  SRR4302532.6071610/1‑94 (MQ=60)
                                                           actatttcagactaaagcgcatctctttttccccgGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGatcccgaa                                   >  SRR4302532.262467/36‑86 (MQ=56)
                                                           ATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAT                                   <  SRR4302532.2405141/94‑1 (MQ=60)
                                                             TGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTT                                 >  SRR4302532.4044155/1‑94 (MQ=60)
                                                              GCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTC                                >  SRR4302532.3966429/1‑94 (MQ=60)
                                                               CAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCC                               >  SRR4302532.638087/1‑94 (MQ=60)
                                                               CAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCC                               <  SRR4302532.2595946/94‑1 (MQ=60)
                                                               CAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCC                               >  SRR4302532.638086/1‑94 (MQ=60)
                                                               CAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCC                               >  SRR4302532.5223254/1‑94 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                              >  SRR4302532.3047315/1‑94 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                              <  SRR4302532.3067761/94‑1 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                              >  SRR4302532.4093531/1‑94 (MQ=60)
                                                                 AATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTT                             <  SRR4302532.3966620/94‑1 (MQ=60)
                                                                   TGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGC                           <  SRR4302532.2950257/94‑1 (MQ=60)
                                                                    GAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCA                          <  SRR4302532.3924177/94‑1 (MQ=60)
                                                                      GGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT                        >  SRR4302532.4259665/1‑94 (MQ=60)
                                                                      GGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT                        >  SRR4302532.5382593/1‑94 (MQ=60)
                                                                        ATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAT                      >  SRR4302532.122899/1‑94 (MQ=60)
                                                                        ATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAT                      <  SRR4302532.4193639/94‑1 (MQ=60)
                                                                        ATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAT                      >  SRR4302532.352049/1‑94 (MQ=60)
                                                                        ATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAT                      <  SRR4302532.1755141/94‑1 (MQ=60)
                                                                        ATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAT                      >  SRR4302532.5491277/1‑94 (MQ=60)
                                                                         TAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATG                     >  SRR4302532.2233427/1‑94 (MQ=60)
                                                                          AAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGT                    >  SRR4302532.1097433/1‑94 (MQ=60)
                                                                          AAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGT                    >  SRR4302532.2776632/1‑94 (MQ=60)
                                                                          AAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGATTATCAGGCCTACATTTCCTTGCAATATGT                    >  SRR4302532.780496/1‑94 (MQ=60)
                                                                           AAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTG                   >  SRR4302532.243119/1‑94 (MQ=60)
                                                                            AAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGC                  >  SRR4302532.5369462/1‑94 (MQ=60)
                                                                            AAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGC                  <  SRR4302532.4567132/94‑1 (MQ=60)
                                                                             AAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA                 >  SRR4302532.3692026/1‑94 (MQ=60)
                                                                             AAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA                 <  SRR4302532.1102529/94‑1 (MQ=60)
                                                                              AAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAT                >  SRR4302532.3128519/1‑94 (MQ=60)
                                                                              AAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAT                <  SRR4302532.5092499/94‑1 (MQ=60)
                                                                              AAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAT                >  SRR4302532.1055537/1‑94 (MQ=60)
                                                                               AACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATT               <  SRR4302532.39292/94‑1 (MQ=60)
                                                                               AACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATT               >  SRR4302532.484243/1‑94 (MQ=60)
                                                                               AACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATT               >  SRR4302532.2658349/1‑94 (MQ=60)
                                                                                ACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTA              <  SRR4302532.2162635/94‑1 (MQ=60)
                                                                                ACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTA              >  SRR4302532.831663/1‑94 (MQ=60)
                                                                                 CCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAC             <  SRR4302532.1401958/94‑1 (MQ=60)
                                                                                  CGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACT            >  SRR4302532.2333286/1‑94 (MQ=60)
                                                                                    GGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTT          >  SRR4302532.581219/1‑94 (MQ=60)
                                                                                    GGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTT          >  SRR4302532.581450/1‑94 (MQ=60)
                                                                                     GTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTG         >  SRR4302532.2212342/1‑94 (MQ=60)
                                                                                        TCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAG      <  SRR4302532.3526949/94‑1 (MQ=60)
                                                                                        TCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAG      <  SRR4302532.3682318/94‑1 (MQ=60)
                                                                                        TCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAG      <  SRR4302532.4942549/94‑1 (MQ=60)
                                                                                         CCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG     >  SRR4302532.479009/1‑94 (MQ=60)
                                                                                          CCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCC   >  SRR4302532.5516054/1‑94 (MQ=60)
                                                                                           CG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG  >  SRR4302532.5301153/1‑94 (MQ=60)
                                                                                              |                                                                                           
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG  >  NC_000913/3510028‑3510212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑