Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,510,121 +G intergenic (‑358/+151) yhfZ ← / ← trpS hypothetical protein/tryptophan tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,510,1201.G95.1% 797.1 / 27.9 226intergenic (‑357/+152)yhfZ/trpShypothetical protein/tryptophan tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base . (7/4);  new base G (129/85);  total (137/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG  >  NC_000913/3510028‑3510209
                                                                                             |                                                                                         
aaGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTcccc                                                                                            >  1:2334051/1‑93 (MQ=255)
 aGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑g                                                                                          >  1:2446399/1‑93 (MQ=255)
  gATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑gg                                                                                         >  2:338925/1‑92 (MQ=255)
    tAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑Ggtt                                                                                       >  2:4184822/1‑93 (MQ=255)
     aaGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑Ggttt                                                                                      <  2:3714145/93‑2 (MQ=255)
     aaGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑Ggttt                                                                                      >  2:333883/1‑92 (MQ=255)
      aGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑Ggtttc                                                                                     >  1:4899188/1‑91 (MQ=255)
      aGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑Ggtttc                                                                                     <  2:4561857/93‑3 (MQ=255)
        gcgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCag                                                                                    >  2:3624592/1‑93 (MQ=255)
         cgcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTGTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCaga                                                                                   >  2:4658932/1‑93 (MQ=255)
          gcAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCagag                                                                                  <  1:5263744/93‑1 (MQ=255)
           cAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGt                                                                                 <  1:3284585/93‑1 (MQ=255)
           cAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGt                                                                                 <  1:2540800/93‑1 (MQ=255)
             aCAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGtga                                                                               >  2:5238793/1‑93 (MQ=255)
              cAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGtgat                                                                              >  1:4170858/1‑93 (MQ=255)
               aGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGtgatg                                                                             >  1:2052474/1‑93 (MQ=255)
               aGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGtgatg                                                                             >  2:3700976/1‑93 (MQ=255)
                gCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGtgatga                                                                            <  2:5606214/93‑1 (MQ=255)
                  gagaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATa                                                                          >  2:1486326/1‑93 (MQ=255)
                    gaTGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATaaa                                                                        >  2:281145/1‑93 (MQ=255)
                      tgtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAGAAg                                                                      >  2:3215665/1‑93 (MQ=255)
                      tgtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAg                                                                      <  2:2162786/93‑1 (MQ=255)
                      tgtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAg                                                                      <  2:2610634/93‑1 (MQ=255)
                       ttagAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGc                                                                     <  2:1250010/90‑1 (MQ=255)
                       gtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGc                                                                     >  2:5218623/1‑93 (MQ=255)
                       gtgGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGc                                                                     <  1:4291203/93‑1 (MQ=255)
                         ggAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCaa                                                                   >  2:1334895/1‑93 (MQ=255)
                          gAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCaaa                                                                  >  1:5160906/1‑93 (MQ=255)
                          gAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCaaa                                                                  >  1:4809121/1‑93 (MQ=255)
                           aaaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCaaaa                                                                 <  2:6494600/93‑1 (MQ=255)
                            aaCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAAt                                                                <  1:1381658/93‑1 (MQ=255)
                             aCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAAtt                                                               <  1:3250221/93‑1 (MQ=255)
                             aCGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAAtt                                                               <  1:5459297/93‑1 (MQ=255)
                               ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGc                                                             >  1:6289011/1‑93 (MQ=255)
                               ggCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGc                                                             >  1:3818128/1‑93 (MQ=255)
                                gCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGcc                                                            >  2:1745134/1‑93 (MQ=255)
                                  gAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTg                                                          >  1:2829001/1‑93 (MQ=255)
                                  gAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGGTTCCCCGGTTTCAGAGTGATGATAAAAGAAAACTTGCCTg                                                          >  2:4839299/1‑93 (MQ=255)
                                    ggCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAt                                                        >  2:4025561/1‑93 (MQ=255)
                                      cACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgc                                                      <  1:3032715/93‑1 (MQ=255)
                                      cACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgc                                                      >  1:1814515/1‑93 (MQ=255)
                                       aCTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcg                                                     >  1:2178283/1‑93 (MQ=255)
                                        cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc                                                    >  2:6070822/1‑93 (MQ=255)
                                        cTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc                                                    <  1:1950888/93‑1 (MQ=255)
                                        cTTCACATATTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATgcgc                                                    >  1:6489217/1‑93 (MQ=255)
                                         ttCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCt                                                   >  1:1689310/1‑93 (MQ=255)
                                          tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                  >  1:5365899/1‑93 (MQ=255)
                                          tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGCTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                  >  2:4877039/1‑93 (MQ=255)
                                          tCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGGTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTa                                                  >  1:6610032/1‑93 (MQ=255)
                                           cacaTTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAc                                                 >  1:273205/1‑93 (MQ=255)
                                               ttttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCtt                                             <  2:2257943/93‑1 (MQ=255)
                                               ttttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCtt                                             >  2:5407943/1‑93 (MQ=255)
                                               ttttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCtt                                             <  2:185338/93‑1 (MQ=255)
                                                tttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTa                                            >  1:728197/1‑93 (MQ=255)
                                                 ttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAt                                           >  1:4392240/1‑93 (MQ=255)
                                                 ttttCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAt                                           >  1:1499890/1‑93 (MQ=255)
                                                       gATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                     <  2:838787/93‑1 (MQ=255)
                                                        attattGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCt                                    <  2:4916455/93‑1 (MQ=255)
                                                        attattGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCt                                    >  2:6579182/1‑93 (MQ=255)
                                                          tattGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAc                                  >  1:4043661/1‑93 (MQ=255)
                                                           attGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                 <  1:3061589/93‑1 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               <  2:6284061/93‑1 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               <  2:5913601/93‑1 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               >  2:2205744/1‑93 (MQ=255)
                                                             tGCAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAtt                               >  1:1364749/1‑93 (MQ=255)
                                                               cAAATGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTc                             >  1:5852401/1‑93 (MQ=255)
                                                                aaaTGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTcc                            <  2:5807600/93‑1 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTTCt                           >  1:5652398/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  1:2146975/1‑93 (MQ=255)
                                                                 aaTGAGGATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCt                           >  1:1474838/1‑93 (MQ=255)
                                                                       gATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                     >  2:1392420/1‑93 (MQ=255)
                                                                       gATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAat                     >  1:1056859/1‑93 (MQ=255)
                                                                        aTAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAata                    <  2:1614527/93‑1 (MQ=255)
                                                                          aaaaaaaCCGGGTTTTCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTCCGCTTATCAGGCCTACACTTCCTTACAttatt                  >  1:3570668/1‑92 (MQ=21)
                                                                          aaaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  >  1:4120989/1‑93 (MQ=255)
                                                                          aaaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtg                  <  1:978544/93‑1 (MQ=255)
                                                                           aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgt                 <  2:243810/93‑1 (MQ=255)
                                                                           aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgt                 >  2:3154119/1‑93 (MQ=255)
                                                                            aaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAATATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                >  2:3566522/1‑93 (MQ=255)
                                                                            aaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAtgtg                >  1:1828659/1‑93 (MQ=255)
                                                                              aaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              >  1:2603154/1‑93 (MQ=255)
                                                                              aaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              >  2:2520150/1‑93 (MQ=255)
                                                                              aaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              <  1:4507052/93‑1 (MQ=255)
                                                                              aaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCa              >  2:5447920/1‑93 (MQ=255)
                                                                               aaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAt             >  2:5037448/1‑93 (MQ=255)
                                                                                aCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtt            >  1:4219362/1‑93 (MQ=255)
                                                                                 ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTa           >  2:4382388/1‑93 (MQ=255)
                                                                                  cGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAc          >  1:4650086/1‑93 (MQ=255)
                                                                                   gggTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACt         <  1:1602047/93‑1 (MQ=255)
                                                                                   gggTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACt         >  1:3525954/1‑93 (MQ=255)
                                                                                     gTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACttt       <  2:1082215/93‑1 (MQ=255)
                                                                                       tccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa    <  2:970360/91‑1 (MQ=255)
                                                                                       tccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa    <  2:2784724/91‑1 (MQ=255)
                                                                                       tccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa    <  1:5787467/91‑1 (MQ=255)
                                                                                        tCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa    >  2:4239776/1‑93 (MQ=255)
                                                                                        ccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAg   >  2:5128620/2‑93 (MQ=255)
                                                                                        ccccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAg   >  1:2686717/2‑93 (MQ=255)
                                                                                         cccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCCGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAgg  >  1:1292768/1‑93 (MQ=255)
                                                                                         cccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAgg  >  2:170530/1‑93 (MQ=255)
                                                                                         cccg‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAgg  >  1:5740449/1‑93 (MQ=255)
                                                                                             |                                                                                         
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGGTTTCCCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG  >  NC_000913/3510028‑3510209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGA  >  NC_000913/3510028‑3510214
                                                                                                 |                                                                                             
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCC‑G                                                                                               >  SRR4302547.2495856/1‑94 (MQ=60)
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCC‑G                                                                                               >  SRR4302547.2338739/1‑94 (MQ=60)
 AGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCC‑GG                                                                                              >  SRR4302547.4650649/1‑94 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCC‑GGTT                                                                                            >  SRR4302547.5756172/1‑94 (MQ=60)
   ATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCC‑GGTT                                                                                            <  SRR4302547.4293295/94‑1 (MQ=60)
     AAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTC                                                                                           >  SRR4302547.6003268/1‑94 (MQ=60)
     AAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTC                                                                                           >  SRR4302547.5000638/1‑94 (MQ=60)
         CGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTTCC‑CCC‑GTTTCAGAG                                                                                       >  SRR4302547.2682370/1‑94 (MQ=60)
          GCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGT                                                                                      <  SRR4302547.5372140/94‑1 (MQ=60)
           CAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTG                                                                                     <  SRR4302547.2592126/94‑1 (MQ=60)
           CAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTG                                                                                     <  SRR4302547.3350460/94‑1 (MQ=60)
           CAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTG                                                                                     <  SRR4302547.5290221/94‑1 (MQ=60)
           CAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTG                                                                                     >  SRR4302547.4287364/1‑94 (MQ=60)
           CAACAGCGAGAAGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTG                                                                                     >  SRR4302547.4142650/1‑94 (MQ=60)
             ACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGAT                                                                                   >  SRR4302547.4255819/1‑94 (MQ=60)
              CAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATG                                                                                  >  SRR4302547.2093409/1‑94 (MQ=60)
               AGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGA                                                                                 <  SRR4302547.2781973/94‑1 (MQ=60)
                GCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGAT                                                                                >  SRR4302547.55161/1‑94 (MQ=60)
                  GAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAA                                                                              >  SRR4302547.3906180/1‑94 (MQ=60)
                  GAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAA                                                                              >  SRR4302547.1835870/1‑94 (MQ=60)
                   AGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAA                                                                             <  SRR4302547.731274/94‑1 (MQ=60)
                   AGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAA                                                                             >  SRR4302547.3238996/1‑94 (MQ=60)
                    GATGTGGAAACGGCGAGGCACTGCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAA                                                                            <  SRR4302547.494967/94‑1 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAG                                                                           <  SRR4302547.4128545/94‑1 (MQ=60)
                     ATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAG                                                                           <  SRR4302547.1420755/94‑1 (MQ=60)
                       GTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCA                                                                         <  SRR4302547.4379406/94‑1 (MQ=60)
                        TGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAA                                                                        <  SRR4302547.2744547/94‑1 (MQ=60)
                        TGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAA                                                                        >  SRR4302547.1399485/1‑94 (MQ=60)
                         GGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAA                                                                       <  SRR4302547.6680783/94‑1 (MQ=60)
                         GGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAA                                                                       >  SRR4302547.4908800/1‑94 (MQ=60)
                         GGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAA                                                                       >  SRR4302547.6287717/1‑94 (MQ=60)
                         GGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAA                                                                       >  SRR4302547.5267170/1‑94 (MQ=60)
                            AACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATT                                                                    <  SRR4302547.1409343/94‑1 (MQ=60)
                             ACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTG                                                                   <  SRR4302547.3315147/94‑1 (MQ=60)
                             ACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTG                                                                   <  SRR4302547.5572089/94‑1 (MQ=60)
                              CGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGC                                                                  >  SRR4302547.6419699/1‑94 (MQ=60)
                              CGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGC                                                                  >  SRR4302547.3895928/1‑94 (MQ=60)
                                GCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCT                                                                <  SRR4302547.1604574/94‑1 (MQ=60)
                                 CGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTG                                                               >  SRR4302547.2885574/1‑94 (MQ=60)
                                 CGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTG                                                               >  SRR4302547.4216036/1‑94 (MQ=60)
                                   AGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGAT                                                             >  SRR4302547.4817489/1‑94 (MQ=60)
                                     GCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGC                                                           >  SRR4302547.1850359/1‑94 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                          <  SRR4302547.3093027/94‑1 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                          <  SRR4302547.1014/94‑1 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                          >  SRR4302547.587977/1‑94 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                          >  SRR4302547.135358/1‑94 (MQ=60)
                                      CACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCG                                                          >  SRR4302547.2221902/1‑94 (MQ=60)
                                       ACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGC                                                         >  SRR4302547.181401/1‑94 (MQ=60)
                                       ACTTCACATATTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGC                                                         >  SRR4302547.6624311/1‑94 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                        <  SRR4302547.1989576/94‑1 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                        >  SRR4302547.1722544/1‑94 (MQ=60)
                                        CTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCT                                                        >  SRR4302547.2871440/1‑94 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       >  SRR4302547.2192158/1‑94 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       <  SRR4302547.1858626/94‑1 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       >  SRR4302547.5476614/1‑94 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       <  SRR4302547.5178419/94‑1 (MQ=60)
                                         TTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑GTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTA                                                       >  SRR4302547.6747623/1‑94 (MQ=60)
                                          TCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTAC                                                      >  SRR4302547.278313/1‑94 (MQ=60)
                                           CACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACG                                                     >  SRR4302547.2567413/1‑94 (MQ=60)
                                            ACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGC                                                    >  SRR4302547.2149522/1‑94 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTT                                                  <  SRR4302547.3227992/94‑1 (MQ=60)
                                              ATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTT                                                  <  SRR4302547.2609717/94‑1 (MQ=60)
                                               TTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTA                                                 >  SRR4302547.741780/1‑94 (MQ=60)
                                                TTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAT                                                >  SRR4302547.1529808/1‑94 (MQ=60)
                                                TTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTAT                                                >  SRR4302547.4482630/1‑94 (MQ=60)
                                                 TTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATC                                               <  SRR4302547.4325473/94‑1 (MQ=60)
                                                 TTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATC                                               >  SRR4302547.562939/1‑94 (MQ=60)
                                                  TTTCAGATTGTTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCA                                              <  SRR4302547.2074904/94‑1 (MQ=60)
                                                    TCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGG                                            <  SRR4302547.922259/94‑1 (MQ=60)
                                                     CGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGC                                           <  SRR4302547.3105386/94‑1 (MQ=60)
                                                     CGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGC                                           >  SRR4302547.6717612/1‑94 (MQ=60)
                                                      GGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCCTATCAGGCC                                          >  SRR4302547.3589538/1‑94 (MQ=60)
                                                       GATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCT                                         >  SRR4302547.2102678/1‑94 (MQ=60)
                                                        ATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTA                                        >  SRR4302547.2949597/1‑94 (MQ=60)
                                                        ATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTA                                        >  SRR4302547.5244481/1‑94 (MQ=60)
                                                         TTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAC                                       >  SRR4302547.4125772/1‑94 (MQ=60)
                                                           ATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAT                                     <  SRR4302547.3122482/94‑1 (MQ=60)
                                                           ATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAT                                     <  SRR4302547.23699/94‑1 (MQ=60)
                                                           ATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAT                                     <  SRR4302547.537473/94‑1 (MQ=60)
                                                           ATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCCACAT                                     >  SRR4302547.5877659/1‑94 (MQ=60)
                                                            TTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATT                                    <  SRR4302547.1627594/94‑1 (MQ=60)
                                                            TTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATT                                    >  SRR4302547.1392136/1‑94 (MQ=60)
                                                              GCAAATGAGGATAAAAAAACCGGG‑TTT‑CTCCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTC                                  <  SRR4302547.1889607/94‑1 (MQ=60)
                                                              GCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTC                                  >  SRR4302547.5973476/1‑94 (MQ=60)
                                                               CAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCC                                 >  SRR4302547.3327236/1‑94 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTTCT                                >  SRR4302547.5769090/1‑94 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                                >  SRR4302547.1504299/1‑94 (MQ=60)
                                                                AAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCT                                >  SRR4302547.2189815/1‑94 (MQ=60)
                                                                 AATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTT                               >  SRR4302547.3450973/1‑94 (MQ=60)
                                                                  ATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTG                              <  SRR4302547.1438009/94‑1 (MQ=60)
                                                                  ATGAGGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTG                              >  SRR4302547.3860465/1‑94 (MQ=60)
                                                                     AGGATAAAAAAACCGGG‑TTT‑C‑CCCAGCTTCAGAGTGATGATAAAAGCGAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAA                           >  SRR4302547.5450932/1‑94 (MQ=60)
                                                                      GGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT                          >  SRR4302547.1077881/1‑94 (MQ=60)
                                                                      GGATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAAT                          >  SRR4302547.2982059/1‑94 (MQ=60)
                                                                       GATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATA                         >  SRR4302547.2497867/1‑94 (MQ=60)
                                                                        ATAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATAT                        >  SRR4302547.5914447/1‑94 (MQ=60)
                                                                         TAAAAAAACCGGGTTTT‑C‑CCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTCCGCTTATCAGGCCTACACTTCCTTacattatt                       >  SRR4302547.3643507/1‑86 (MQ=55)
                                                                         TAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATG                       >  SRR4302547.4425318/1‑94 (MQ=60)
                                                                         TAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATG                       >  SRR4302547.4204733/1‑94 (MQ=60)
                                                                         TAAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATG                       <  SRR4302547.3867464/94‑1 (MQ=60)
                                                                          AAAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGT                      <  SRR4302547.997569/94‑1 (MQ=60)
                                                                           AAAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTG                     >  SRR4302547.1864806/1‑94 (MQ=60)
                                                                            AAAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGC                    >  SRR4302547.3607712/1‑94 (MQ=60)
                                                                             AAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA                   >  SRR4302547.1880500/1‑94 (MQ=60)
                                                                             AAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA                   >  SRR4302547.2655618/1‑94 (MQ=60)
                                                                             AAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA                   >  SRR4302547.1347014/1‑94 (MQ=60)
                                                                             AAAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA                   >  SRR4302547.1700628/1‑94 (MQ=60)
                                                                              AAACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAT                  <  SRR4302547.4600159/94‑1 (MQ=60)
                                                                               AACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATT                 >  SRR4302547.4305530/1‑94 (MQ=60)
                                                                               AACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATT                 <  SRR4302547.919666/94‑1 (MQ=60)
                                                                                ACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTA                >  SRR4302547.1517162/1‑94 (MQ=60)
                                                                                ACCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTA                <  SRR4302547.2433862/94‑1 (MQ=60)
                                                                                 CCGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAC               >  SRR4302547.4746408/1‑94 (MQ=60)
                                                                                  CGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACT              >  SRR4302547.3597943/1‑94 (MQ=60)
                                                                                  CGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACT              >  SRR4302547.4938679/1‑94 (MQ=60)
                                                                                  CGGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACT              >  SRR4302547.2592624/1‑94 (MQ=60)
                                                                                   GGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTT             <  SRR4302547.1633822/94‑1 (MQ=60)
                                                                                   GGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTT             >  SRR4302547.4525097/1‑94 (MQ=60)
                                                                                   GGG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTT             >  SRR4302547.4506036/1‑94 (MQ=60)
                                                                                    GG‑TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCTTACTTTTCCTTGCATTATGTcaattctttt            >  SRR4302547.3284668/1‑84 (MQ=53)
                                                                                       TTT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGT          <  SRR4302547.4411787/94‑1 (MQ=60)
                                                                                        TT‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTA         <  SRR4302547.4841209/94‑1 (MQ=60)
                                                                                         T‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTGCTTTGTAG        <  SRR4302547.1093290/94‑1 (MQ=60)
                                                                                         T‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAG        >  SRR4302547.2740648/1‑94 (MQ=60)
                                                                                         T‑C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAG        <  SRR4302547.5907178/94‑1 (MQ=60)
                                                                                           C‑CCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGC      >  SRR4302547.2394379/1‑94 (MQ=60)
                                                                                           C‑CCG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGC      >  SRR4302547.3815319/1‑94 (MQ=60)
                                                                                           C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCCGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG       >  SRR4302547.1318791/1‑94 (MQ=60)
                                                                                           C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG       <  SRR4302547.6439966/94‑1 (MQ=60)
                                                                                           C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG       <  SRR4302547.5787011/94‑1 (MQ=60)
                                                                                           C‑CCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGG       >  SRR4302547.5859078/1‑94 (MQ=60)
                                                                                              CG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG    <  SRR4302547.2578847/94‑1 (MQ=60)
                                                                                              CG‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCG    >  SRR4302547.3998509/1‑94 (MQ=60)
                                                                                               G‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGG   >  SRR4302547.2483823/1‑94 (MQ=60)
                                                                                                 GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGA  <  SRR4302547.2442567/94‑1 (MQ=60)
                                                                                                 |                                                                                             
AAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAATGAGGATAAAAAAACCGGG‑TTT‑C‑CCC‑GTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGA  >  NC_000913/3510028‑3510214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑