Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,790,609 G→A D239D (GAC→GAT)  htrL ← HtrL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,6090GA99.5% 821.8 / ‑5.5 218D239D (GAC→GAT) htrLHtrL
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (103/114);  total (103/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 220 total aligned reads displayed.

GCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGGTCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAAC  >  NC_000913/3790519‑3790698
                                                                                          |                                                                                         
gcGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATc                                                                                         <  1:5439383/93‑1 (MQ=255)
gcGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATc                                                                                         <  2:1690671/93‑1 (MQ=255)
  gAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCAt                                                                                       >  1:5363679/1‑93 (MQ=255)
  gAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCAt                                                                                       >  2:5810913/1‑93 (MQ=255)
  gAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCAt                                                                                       >  2:5044985/1‑93 (MQ=255)
  gAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCAt                                                                                       <  2:2351233/93‑1 (MQ=255)
    aaCAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCg                                                                                     >  2:3191793/1‑93 (MQ=255)
      cAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTc                                                                                   >  2:3878324/1‑93 (MQ=255)
          tCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCcaca                                                                               >  1:1960513/1‑93 (MQ=255)
           cGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACaa                                                                              <  2:3738157/93‑1 (MQ=255)
           cGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACaa                                                                              <  2:1573861/93‑1 (MQ=255)
            gAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAAt                                                                             <  2:4459995/93‑1 (MQ=255)
             aaCCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATg                                                                            >  2:4236231/1‑93 (MQ=255)
              aCCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGt                                                                           >  1:4388941/1‑93 (MQ=255)
              aCCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGt                                                                           <  2:106268/93‑1 (MQ=255)
              aCCATTTTCCTCCCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGGTCATCGTCCACAATGt                                                                           <  2:5131200/93‑1 (MQ=255)
               ccATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGtt                                                                          <  1:2809376/93‑1 (MQ=255)
               ccATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGtt                                                                          <  1:1909559/93‑1 (MQ=255)
                cATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGtta                                                                         <  1:234335/93‑1 (MQ=255)
                 aTTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGttat                                                                        <  1:5976561/93‑1 (MQ=255)
                 aTTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGttat                                                                        <  2:2636409/93‑1 (MQ=255)
                 aTTTTCCGCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGttat                                                                        <  1:2158721/93‑1 (MQ=255)
                  ttttCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGttatt                                                                       <  1:5715354/93‑1 (MQ=255)
                   tttCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGttatta                                                                      <  2:5631054/93‑1 (MQ=255)
                   tttCCTCCCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGGTTGGAATAGCACATGACAAAAATGCCCTGGTCATCGTCCACAATGttatta                                                                      >  1:3334707/1‑93 (MQ=255)
                    ttCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTtattat                                                                     <  1:1839187/93‑1 (MQ=255)
                     tCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTattatt                                                                    >  1:6143422/1‑93 (MQ=255)
                     tCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTattatt                                                                    >  2:4927419/1‑93 (MQ=255)
                      ccTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTAttt                                                                   <  1:5429200/93‑1 (MQ=255)
                       ctctCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTa                                                                  <  2:2759154/93‑1 (MQ=255)
                        tctcCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAg                                                                 >  1:1141547/1‑93 (MQ=255)
                        tctcCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAg                                                                 <  2:2538325/93‑1 (MQ=255)
                        tctcCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAg                                                                 <  2:6134449/93‑1 (MQ=255)
                        tctcCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAg                                                                 >  2:5546779/1‑93 (MQ=255)
                          tcCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAgtg                                                               <  1:4861969/93‑1 (MQ=255)
                            cccAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGtt                                                             >  2:5655847/1‑93 (MQ=255)
                             ccAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTa                                                            >  2:929495/1‑93 (MQ=255)
                              cAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAt                                                           >  2:470247/1‑93 (MQ=255)
                              cAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAt                                                           >  1:4698903/1‑93 (MQ=255)
                               aGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAtt                                                          >  1:4966780/1‑93 (MQ=255)
                                gATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAttt                                                         >  1:151521/1‑93 (MQ=255)
                                gATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAttt                                                         >  2:4035162/1‑93 (MQ=255)
                                  tAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAttttt                                                       <  2:2420732/93‑1 (MQ=255)
                                   aGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAtttttt                                                      <  2:1185866/93‑1 (MQ=255)
                                   aGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAtttttt                                                      <  1:5186602/93‑1 (MQ=255)
                                    gTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTg                                                     >  2:2197263/1‑93 (MQ=255)
                                     tttAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGa                                                    >  2:4798528/1‑93 (MQ=255)
                                     tttAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGa                                                    >  2:2786881/1‑93 (MQ=255)
                                       tAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACt                                                  >  1:3548774/1‑93 (MQ=255)
                                       tAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACt                                                  >  1:775953/1‑93 (MQ=255)
                                        aGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACtt                                                 <  2:3007097/93‑1 (MQ=255)
                                         ggTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACttt                                                >  2:5739263/1‑93 (MQ=255)
                                          gTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTc                                               <  2:3695694/93‑1 (MQ=255)
                                           ttGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTcc                                              <  1:310770/93‑1 (MQ=255)
                                           ttGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTcc                                              >  2:344316/1‑93 (MQ=255)
                                            tGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCa                                             <  2:4578420/93‑1 (MQ=255)
                                            tGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCa                                             <  2:3035023/93‑1 (MQ=255)
                                            tGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCa                                             <  1:5624544/93‑1 (MQ=255)
                                                gagaTCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAAc                                         >  1:3006368/1‑93 (MQ=255)
                                                  gaTCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTa                                       >  1:2469834/1‑93 (MQ=255)
                                                  gaTCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTa                                       >  2:3520110/1‑93 (MQ=255)
                                                   aTCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTaa                                      <  1:2254995/93‑1 (MQ=255)
                                                   aTCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTaa                                      <  1:1248079/93‑1 (MQ=255)
                                                    tCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAAt                                     <  1:2073341/93‑1 (MQ=255)
                                                    tCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAAt                                     >  2:4766782/1‑93 (MQ=255)
                                                      ggggCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAAttt                                   >  2:2888291/1‑93 (MQ=255)
                                                      ggggCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAAttt                                   >  1:3706896/1‑93 (MQ=255)
                                                      ggggCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAAttt                                   >  2:1524548/1‑93 (MQ=255)
                                                       gggCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTta                                  <  2:2066895/93‑1 (MQ=255)
                                                        ggCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTtat                                 <  2:2288830/93‑1 (MQ=255)
                                                         gCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTtata                                <  2:3794302/93‑1 (MQ=255)
                                                          cGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAg                               >  1:1690060/1‑93 (MQ=255)
                                                           gTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGa                              >  2:3670979/1‑93 (MQ=255)
                                                                tAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCt                         <  2:6008546/93‑1 (MQ=255)
                                                                tAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCt                         <  1:5044350/93‑1 (MQ=255)
                                                                tAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCt                         <  2:765419/93‑1 (MQ=255)
                                                                 aaTAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCtt                        <  1:425318/93‑1 (MQ=255)
                                                                 aaTAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCtt                        <  1:3165357/93‑1 (MQ=255)
                                                                  aTAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCttt                       <  2:5052104/93‑1 (MQ=255)
                                                                   tAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTc                      <  1:5728990/93‑1 (MQ=255)
                                                                    aGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTcc                     <  1:2562242/93‑1 (MQ=255)
                                                                     gCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCa                    <  1:1234652/93‑1 (MQ=255)
                                                                      cacaTGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCAt                   <  2:1865300/93‑1 (MQ=255)
                                                                        caTGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCAttt                 >  1:2686505/1‑93 (MQ=255)
                                                                         aTGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTa                <  2:4694175/93‑1 (MQ=255)
                                                                         aTGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTa                <  2:638964/93‑1 (MQ=255)
                                                                         aTGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTa                <  1:2851725/93‑1 (MQ=255)
                                                                         aTGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTa                <  1:376818/93‑1 (MQ=255)
                                                                         aTGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTa                >  2:5752576/1‑93 (MQ=255)
                                                                          tGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTAt               >  2:4454042/1‑93 (MQ=255)
                                                                               aaaaTGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCtgt          <  1:942864/93‑1 (MQ=255)
                                                                               aaaaTGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCtgt          >  1:4190675/1‑93 (MQ=255)
                                                                               aaaaTGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCtgt          >  1:4041715/1‑93 (MQ=255)
                                                                                aaaTGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCtgtg         >  1:1723147/1‑93 (MQ=255)
                                                                                   tGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAAc      <  1:4178585/93‑1 (MQ=255)
                                                                                   tGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAAc      >  2:1309330/1‑93 (MQ=255)
                                                                                    gCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAAca     >  1:4457944/1‑92 (MQ=255)
                                                                                      ccTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACcaa   >  1:6016444/1‑93 (MQ=255)
                                                                                      ccTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACcaa   <  2:3547656/93‑1 (MQ=255)
                                                                                       cTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACcaac  <  1:3845386/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGGTCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAAC  >  NC_000913/3790519‑3790698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGGTCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATT  >  NC_000913/3790516‑3790702
                                                                                             |                                                                                             
GCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGA                                                                                               >  SRR4302532.5026504/1‑94 (MQ=60)
 CAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGAT                                                                                              <  SRR4302532.24096/94‑1 (MQ=60)
 CAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGAT                                                                                              <  SRR4302532.24095/94‑1 (MQ=60)
 CAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGAT                                                                                              >  SRR4302532.2535640/1‑94 (MQ=60)
 CAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGAT                                                                                              <  SRR4302532.2580074/94‑1 (MQ=60)
 CAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGAT                                                                                              <  SRR4302532.3716477/94‑1 (MQ=60)
   GCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCA                                                                                            <  SRR4302532.5627892/94‑1 (MQ=60)
    CGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCAT                                                                                           >  SRR4302532.5549610/1‑94 (MQ=60)
    CGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCAT                                                                                           <  SRR4302532.2680920/94‑1 (MQ=60)
     GAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATC                                                                                          >  SRR4302532.5551243/1‑94 (MQ=60)
     GAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATC                                                                                          <  SRR4302532.1980375/94‑1 (MQ=60)
         CAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCC                                                                                      <  SRR4302532.4829439/94‑1 (MQ=60)
           AATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCAC                                                                                    >  SRR4302532.860486/1‑94 (MQ=60)
           AATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCAC                                                                                    <  SRR4302532.2187860/94‑1 (MQ=60)
            ATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACA                                                                                   >  SRR4302532.2025639/1‑94 (MQ=60)
              CGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAAT                                                                                 >  SRR4302532.1766270/1‑94 (MQ=60)
               GAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATG                                                                                >  SRR4302532.4427206/1‑94 (MQ=60)
                AACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGT                                                                               >  SRR4302532.4538120/1‑94 (MQ=60)
                AACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGT                                                                               <  SRR4302532.3214833/94‑1 (MQ=60)
                 ACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCGCTGATCATCGTCCATAATGTT                                                                              <  SRR4302532.3259642/94‑1 (MQ=60)
                 ACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTT                                                                              <  SRR4302532.2908415/94‑1 (MQ=60)
                  CCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTA                                                                             >  SRR4302532.1357420/1‑94 (MQ=60)
                  CCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTA                                                                             <  SRR4302532.2903441/94‑1 (MQ=60)
                  CCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTA                                                                             <  SRR4302532.1972964/94‑1 (MQ=60)
                   CATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTAT                                                                            <  SRR4302532.242355/94‑1 (MQ=60)
                    ATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATT                                                                           <  SRR4302532.6183134/94‑1 (MQ=60)
                    ATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATT                                                                           >  SRR4302532.4167910/1‑94 (MQ=60)
                    ATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATT                                                                           <  SRR4302532.510406/94‑1 (MQ=60)
                    ATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATT                                                                           <  SRR4302532.4182038/94‑1 (MQ=60)
                    ATTTTCCGCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATT                                                                           <  SRR4302532.2230885/94‑1 (MQ=60)
                     TTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTA                                                                          <  SRR4302532.5914488/94‑1 (MQ=60)
                     TTTTCCTCCCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGGTTGGAATAGCACATGACAAAAATGCCCTGGTCATCGTCCACAATGTTATTA                                                                          >  SRR4302532.3445952/1‑94 (MQ=60)
                       TTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATT                                                                        <  SRR4302532.1900295/94‑1 (MQ=60)
                       TTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATT                                                                        >  SRR4302532.6355327/1‑94 (MQ=60)
                       TTCCACTCCCCAGATAGTTTAGGTTGAAGAGATCGGTGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATT                                                                        <  SRR4302532.6298044/94‑1 (MQ=60)
                        TCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTT                                                                       <  SRR4302532.2378399/94‑1 (MQ=60)
                         CCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTA                                                                      <  SRR4302532.5617359/94‑1 (MQ=60)
                         CCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTA                                                                      >  SRR4302532.3070330/1‑94 (MQ=60)
                          CTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAG                                                                     >  SRR4302532.1179614/1‑94 (MQ=60)
                            CTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTG                                                                   >  SRR4302532.1925521/1‑94 (MQ=60)
                             TCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGT                                                                  <  SRR4302532.5029214/94‑1 (MQ=60)
                              CCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTT                                                                 <  SRR4302532.2095175/94‑1 (MQ=60)
                              CCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTT                                                                 <  SRR4302532.3845461/94‑1 (MQ=60)
                                CCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTAT                                                               >  SRR4302532.4859183/1‑94 (MQ=60)
                                 CAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATT                                                              >  SRR4302532.5137080/1‑94 (MQ=60)
                                  AGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTT                                                             >  SRR4302532.156593/1‑94 (MQ=60)
                                    ATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTT                                                           >  SRR4302532.5205218/1‑94 (MQ=60)
                                      AGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTG                                                         <  SRR4302532.5365313/94‑1 (MQ=60)
                                         TTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACT                                                      >  SRR4302532.3667513/1‑94 (MQ=60)
                                         TTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACT                                                      >  SRR4302532.802673/1‑94 (MQ=60)
                                              TTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCA                                                 <  SRR4302532.321553/94‑1 (MQ=60)
                                               TGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAG                                                <  SRR4302532.3152606/94‑1 (MQ=60)
                                               TGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAG                                                <  SRR4302532.5819724/94‑1 (MQ=60)
                                               TGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAG                                                <  SRR4302532.4884590/94‑1 (MQ=60)
                                                 AAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAA                                              <  SRR4302532.2196992/94‑1 (MQ=60)
                                                  AGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAAC                                             >  SRR4302532.3105624/1‑94 (MQ=60)
                                                  AGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAAC                                             >  SRR4302532.3651689/1‑94 (MQ=60)
                                                   GAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACT                                            <  SRR4302532.2794524/94‑1 (MQ=60)
                                                   GAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACT                                            <  SRR4302532.5420234/94‑1 (MQ=60)
                                                    AGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTA                                           >  SRR4302532.2552063/1‑94 (MQ=60)
                                                      ATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAAT                                         <  SRR4302532.1289836/94‑1 (MQ=60)
                                                      ATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAAT                                         <  SRR4302532.2329850/94‑1 (MQ=60)
                                                      ATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAAT                                         <  SRR4302532.2289840/94‑1 (MQ=60)
                                                       TCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATT                                        <  SRR4302532.1786709/94‑1 (MQ=60)
                                                       TCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATT                                        <  SRR4302532.2142438/94‑1 (MQ=60)
                                                        CGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTT                                       >  SRR4302532.3831340/1‑94 (MQ=60)
                                                          GGGCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTAT                                     <  SRR4302532.5618254/94‑1 (MQ=60)
                                                            GCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAG                                   >  SRR4302532.1747131/1‑94 (MQ=60)
                                                            GCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAG                                   >  SRR4302532.5175008/1‑94 (MQ=60)
                                                            GCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAG                                   <  SRR4302532.5821344/94‑1 (MQ=60)
                                                            GCGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAG                                   <  SRR4302532.592140/94‑1 (MQ=60)
                                                             CGTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGA                                  >  SRR4302532.3900404/1‑94 (MQ=60)
                                                              GTTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAA                                 >  SRR4302532.6184750/1‑94 (MQ=60)
                                                               TTTGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAAT                                <  SRR4302532.1206036/94‑1 (MQ=60)
                                                                 TGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTC                              >  SRR4302532.3822701/1‑94 (MQ=60)
                                                                 TGTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTC                              <  SRR4302532.264413/94‑1 (MQ=60)
                                                                  GTAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCT                             >  SRR4302532.6268043/1‑94 (MQ=60)
                                                                  GTAATAGCACATGACAAAAATGCCCCGATCATCGTCCACAACGGGATTATTTAGTGTTAgtgggggacggtccagaactaattgatagaattca                             >  SRR4302532.5642095/1‑59 (MQ=34)
                                                                  GTAATAGCACATGACAAAAATACCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCT                             <  SRR4302532.4372734/94‑1 (MQ=60)
                                                                   TAATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTT                            <  SRR4302532.5217392/94‑1 (MQ=60)
                                                                    AATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTT                           <  SRR4302532.440053/94‑1 (MQ=60)
                                                                    AATAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTT                           <  SRR4302532.3270477/94‑1 (MQ=60)
                                                                      TAGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCC                         <  SRR4302532.5928512/94‑1 (MQ=60)
                                                                       AGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCA                        >  SRR4302532.5299517/1‑94 (MQ=60)
                                                                       AGCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCA                        <  SRR4302532.2647735/94‑1 (MQ=60)
                                                                        GCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCAT                       <  SRR4302532.1275956/94‑1 (MQ=60)
                                                                        GCACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCAT                       <  SRR4302532.1275957/94‑1 (MQ=60)
                                                                          ACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTT                     <  SRR4302532.1008937/94‑1 (MQ=60)
                                                                          ACATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTT                     >  SRR4302532.2776740/1‑94 (MQ=60)
                                                                            aagtCAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTAT                   >  SRR4302532.2737710/5‑94 (MQ=60)
                                                                            ATGACAAAAATGCCTTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTAT                   <  SRR4302532.1594386/94‑1 (MQ=60)
                                                                            ATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTAT                   <  SRR4302532.389905/94‑1 (MQ=60)
                                                                            ATGACAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTAT                   <  SRR4302532.2947065/94‑1 (MQ=60)
                                                                                CAAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTG               >  SRR4302532.2539589/1‑94 (MQ=60)
                                                                                 AAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGT              <  SRR4302532.6119435/94‑1 (MQ=60)
                                                                                 AAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGT              >  SRR4302532.4331290/1‑94 (MQ=60)
                                                                                 AAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGT              >  SRR4302532.4177842/1‑94 (MQ=60)
                                                                                 AAAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGT              >  SRR4302532.4331289/1‑94 (MQ=60)
                                                                                  AAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTG             <  SRR4302532.975157/94‑1 (MQ=60)
                                                                                  AAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTG             >  SRR4302532.4497250/1‑94 (MQ=60)
                                                                                  AAAATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTG             >  SRR4302532.1781181/1‑94 (MQ=60)
                                                                                     ATGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAAC          <  SRR4302532.5629278/94‑1 (MQ=60)
                                                                                      TGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACC         <  SRR4302532.4318749/94‑1 (MQ=60)
                                                                                      TGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACC         >  SRR4302532.3928180/1‑94 (MQ=60)
                                                                                      TGCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACA         >  SRR4302532.4609904/1‑94 (MQ=60)
                                                                                       GCCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCA        >  SRR4302532.4547173/1‑94 (MQ=60)
                                                                                        CCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAA       >  SRR4302532.6224211/1‑94 (MQ=60)
                                                                                        CCCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAA       >  SRR4302532.3377439/1‑94 (MQ=60)
                                                                                         CCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAAC      >  SRR4302532.1769029/1‑94 (MQ=60)
                                                                                         CCTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAAC      >  SRR4302532.777257/1‑94 (MQ=60)
                                                                                          CTGATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACA     <  SRR4302532.3975222/94‑1 (MQ=60)
                                                                                             GTCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATT  >  SRR4302532.5308130/1‑94 (MQ=60)
                                                                                             ATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATT  >  SRR4302532.4122346/1‑94 (MQ=60)
                                                                                             ATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATT  <  SRR4302532.4638844/94‑1 (MQ=60)
                                                                                             ATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATT  >  SRR4302532.1244371/1‑94 (MQ=60)
                                                                                             ATCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATT  >  SRR4302532.1036423/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAGCACATGACAAAAATGCCCTGGTCATCGTCCACAATGTTATTATTTAGTGTTATTTTTTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATT  >  NC_000913/3790516‑3790702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑