Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,799,298 T→C Y110C (TAC→TGC)  rfaJ ← UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,799,2980TC83.4% 655.2 / 119.3 265Y110C (TAC→TGC) rfaJUDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (20/24);  new base C (115/106);  total (135/130)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 271 total aligned reads displayed.

GGCTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGC  >  NC_000913/3799206‑3799387
                                                                                            |                                                                                         
   tAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGcaca                                                                                        <  1:4450372/93‑1 (MQ=255)
   tAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACa                                                                                        >  1:4165898/1‑93 (MQ=255)
    aaTATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACAt                                                                                       <  1:2136623/93‑1 (MQ=255)
     atatCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACAtt                                                                                      >  1:330189/1‑93 (MQ=255)
     atatCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACAtt                                                                                      <  2:4935729/93‑1 (MQ=255)
      tatCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTg                                                                                     >  2:1761344/1‑93 (MQ=255)
       atCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGc                                                                                    <  1:103831/93‑1 (MQ=255)
       atCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGc                                                                                    <  2:1689813/93‑1 (MQ=255)
         cGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGctc                                                                                  >  2:5970773/1‑93 (MQ=255)
         cGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGctc                                                                                  <  2:3156060/93‑1 (MQ=255)
         cGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGctc                                                                                  >  2:5788690/1‑93 (MQ=255)
         cGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGctc                                                                                  >  1:3879223/1‑93 (MQ=255)
           ccTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCtt                                                                                >  2:219155/1‑93 (MQ=255)
           ccTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCtt                                                                                <  2:2679095/93‑1 (MQ=255)
           ccTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCtt                                                                                <  2:4264095/93‑1 (MQ=255)
             tttACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGa                                                                              <  1:1792481/93‑1 (MQ=255)
              ttACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGAc                                                                             <  1:6560853/93‑1 (MQ=255)
                acaaacaaCATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCa                                                                           <  1:707458/93‑1 (MQ=255)
                   aacaacATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGAc                                                                        >  1:2686260/1‑93 (MQ=255)
                   aacaacATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGAc                                                                        >  2:5572998/1‑93 (MQ=255)
                    acaacaTCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGAcc                                                                       <  1:1192622/93‑1 (MQ=255)
                      aacaTCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCtg                                                                     >  1:2055356/1‑93 (MQ=255)
                        caTCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCtgtg                                                                   >  1:597158/1‑93 (MQ=255)
                         aTCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTgtgt                                                                  >  2:1578004/1‑93 (MQ=255)
                         aTCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTgtgt                                                                  >  1:1546319/1‑93 (MQ=255)
                            tGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACa                                                               >  2:6175778/1‑93 (MQ=255)
                            tGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACa                                                               >  2:3530302/1‑93 (MQ=255)
                             gCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACaa                                                              >  2:1345398/1‑93 (MQ=255)
                             gCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACaa                                                              >  2:4525577/1‑93 (MQ=255)
                              cATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAg                                                             <  2:3335311/93‑1 (MQ=255)
                              cATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAg                                                             >  1:4584518/1‑93 (MQ=255)
                              cATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAg                                                             >  2:3036947/1‑93 (MQ=255)
                                tCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGc                                                           >  2:835632/1‑93 (MQ=255)
                                tCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGc                                                           >  2:2589691/1‑93 (MQ=255)
                                tCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGc                                                           >  1:5882144/1‑93 (MQ=255)
                                tCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGc                                                           >  1:643719/1‑93 (MQ=255)
                                 cAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCa                                                          <  1:40311/93‑1 (MQ=255)
                                  aaGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCaa                                                         <  2:6435213/93‑1 (MQ=255)
                                        aaGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTg                                                   >  1:3639916/1‑93 (MQ=255)
                                        aaGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTg                                                   >  2:3790919/1‑93 (MQ=255)
                                          gCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGa                                                 >  2:3431811/1‑93 (MQ=255)
                                           cAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAg                                                >  2:4953647/1‑93 (MQ=255)
                                            aaaCGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGc                                               <  1:2113911/93‑1 (MQ=255)
                                              aCGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCtt                                             >  1:6212741/1‑93 (MQ=255)
                                                gATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTAt                                           <  1:2734766/93‑1 (MQ=255)
                                                gATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCCGTGTACAAGGCAAGCACTGGAGCTTAt                                           <  2:6542687/93‑1 (MQ=255)
                                                 aTCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATc                                          <  2:6069886/93‑1 (MQ=255)
                                                  tCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCa                                         <  2:572359/93‑1 (MQ=255)
                                                   cAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAg                                        >  1:3546429/1‑93 (MQ=255)
                                                    aaGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAgt                                       <  2:2946084/93‑1 (MQ=255)
                                                    aaGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAgt                                       >  1:4969428/1‑93 (MQ=255)
                                                    aaGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAgt                                       <  1:3830724/93‑1 (MQ=255)
                                                    aaGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAgt                                       >  1:2553550/1‑93 (MQ=255)
                                                     aGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAgtg                                      >  2:4861013/1‑93 (MQ=255)
                                                     aGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAgtg                                      <  1:3264418/93‑1 (MQ=255)
                                                       cGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGtt                                    <  1:6091296/93‑1 (MQ=255)
                                                        gTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTGCATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTa                                   <  2:5189984/93‑1 (MQ=255)
                                                        gTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTa                                   <  1:4020605/93‑1 (MQ=255)
                                                            aaCCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTc                               <  1:170839/93‑1 (MQ=255)
                                                              cccAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCta                             <  2:1897348/93‑1 (MQ=255)
                                                              cccAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCta                             <  1:1646938/93‑1 (MQ=255)
                                                               ccAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCtat                            >  1:2900374/1‑93 (MQ=255)
                                                               ccAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCtat                            <  2:1229161/93‑1 (MQ=255)
                                                                 aataatTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCtatat                          >  2:3646120/1‑93 (MQ=255)
                                                                   taatTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATaa                        <  1:6167707/93‑1 (MQ=255)
                                                                    aatTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAAc                       <  1:5201616/93‑1 (MQ=255)
                                                                    aatTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAAc                       >  1:5472949/1‑93 (MQ=255)
                                                                    aatTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAAc                       <  1:72211/93‑1 (MQ=255)
                                                                    aatTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAAc                       >  2:3864763/1‑93 (MQ=255)
                                                                    aatTGAAATGCAAATAAACGGAAGCACATTGATCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAAc                       <  2:4583568/93‑1 (MQ=255)
                                                                     atTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACg                      <  1:5027389/93‑1 (MQ=255)
                                                                     atTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACg                      >  1:3586491/1‑93 (MQ=255)
                                                                     atTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACg                      >  2:2301528/1‑93 (MQ=255)
                                                                     atTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACg                      <  1:5442092/93‑1 (MQ=255)
                                                                       tGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTg                    >  2:4374762/1‑93 (MQ=255)
                                                                       tGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTg                    >  2:5900436/1‑93 (MQ=255)
                                                                         aaaTGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGAt                  <  2:5702876/93‑1 (MQ=255)
                                                                         aaaTGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGAt                  >  2:1371142/1‑93 (MQ=255)
                                                                         aaaTGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGAt                  <  1:3091437/93‑1 (MQ=255)
                                                                          aaTGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGAtt                 >  2:6619349/1‑93 (MQ=255)
                                                                           aTGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTc                >  1:4856111/1‑93 (MQ=255)
                                                                               aaaTAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTaa            <  2:3498884/93‑1 (MQ=255)
                                                                               aaaTAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTaa            >  1:5818236/1‑93 (MQ=255)
                                                                               aaaTAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTaa            >  1:4699456/1‑93 (MQ=255)
                                                                               aaaTAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTaa            <  2:3235745/93‑1 (MQ=255)
                                                                               aaaTAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTaa            <  2:1617934/93‑1 (MQ=255)
                                                                                 aTAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAAtt          >  2:1497508/1‑93 (MQ=255)
                                                                                  tAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTg         <  1:1125048/93‑1 (MQ=255)
                                                                                  tAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTg         <  2:4330556/93‑1 (MQ=255)
                                                                                  tAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTg         >  2:929133/1‑93 (MQ=255)
                                                                                  tAAACGGAAGCACATTGCTCTTGACCAGACCTGTGGACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCCTAATTg         >  1:1274348/1‑93 (MQ=255)
                                                                                   aaaCGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTgg        <  1:992332/93‑1 (MQ=255)
                                                                                   aaaCGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTgg        >  1:6597250/1‑93 (MQ=255)
                                                                                      cGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGttt     >  2:2005067/1‑93 (MQ=255)
                                                                                       ggAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGtttt    <  2:4189628/93‑1 (MQ=255)
                                                                                       ggAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGtttt    <  1:1344975/93‑1 (MQ=255)
                                                                                       ggAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGtttt    >  2:1081915/1‑93 (MQ=255)
                                                                                       ggAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGtttt    <  1:5362866/93‑1 (MQ=255)
                                                                                        gAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTg   >  2:6291387/1‑93 (MQ=255)
                                                                                         aaGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGc  <  2:694766/93‑1 (MQ=255)
                                                                                            |                                                                                         
GGCTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGC  >  NC_000913/3799206‑3799387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTCTG  >  NC_000913/3799205‑3799391
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TGGCTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTCAACCCAATAATTGAAATGCAAATACACGGAAGT                                                                                               >  SRR4302547.2917193/1‑94 (MQ=60)
TGGCTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGC                                                                                               <  SRR4302547.5000805/94‑1 (MQ=60)
   CTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACA                                                                                            >  SRR4302547.4250747/1‑94 (MQ=60)
   CTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACA                                                                                            >  SRR4302547.2203822/1‑94 (MQ=60)
   CTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACA                                                                                            <  SRR4302547.5577888/94‑1 (MQ=60)
   CTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACA                                                                                            <  SRR4302547.4706393/94‑1 (MQ=60)
    TAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACAT                                                                                           >  SRR4302547.2877/1‑94 (MQ=60)
    TAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACAT                                                                                           >  SRR4302547.6027040/1‑94 (MQ=60)
    TAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACAT                                                                                           <  SRR4302547.4542382/94‑1 (MQ=60)
     AATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATT                                                                                          >  SRR4302547.336334/1‑94 (MQ=60)
     AATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATT                                                                                          <  SRR4302547.2179185/94‑1 (MQ=60)
        ATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCT                                                                                       >  SRR4302547.4638844/1‑94 (MQ=60)
        ATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCT                                                                                       <  SRR4302547.105727/94‑1 (MQ=60)
         TCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTC                                                                                      >  SRR4302547.3958156/1‑94 (MQ=60)
         TCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTC                                                                                      <  SRR4302547.730454/94‑1 (MQ=60)
          CGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCT                                                                                     >  SRR4302547.5599445/1‑94 (MQ=60)
           GCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTT                                                                                    >  SRR4302547.5071501/1‑94 (MQ=60)
           GCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTT                                                                                    >  SRR4302547.4771609/1‑94 (MQ=60)
           GCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTT                                                                                    <  SRR4302547.945613/94‑1 (MQ=60)
            CCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTG                                                                                   >  SRR4302547.6160261/1‑94 (MQ=60)
              TTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGAC                                                                                 <  SRR4302547.1827967/94‑1 (MQ=60)
              TTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGAC                                                                                 <  SRR4302547.6236892/94‑1 (MQ=60)
               TTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACC                                                                                <  SRR4302547.6697472/94‑1 (MQ=60)
                TACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCA                                                                               >  SRR4302547.2960626/1‑94 (MQ=60)
                TACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCA                                                                               <  SRR4302547.3248414/94‑1 (MQ=60)
                TACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCA                                                                               >  SRR4302547.6647870/1‑94 (MQ=60)
                 ACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAG                                                                              <  SRR4302547.720540/94‑1 (MQ=60)
                  CAAACAACATCCGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGA                                                                             >  SRR4302547.493646/1‑94 (MQ=60)
                   AAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGAC                                                                            >  SRR4302547.2740182/1‑94 (MQ=60)
                    AACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACC                                                                           <  SRR4302547.3762385/94‑1 (MQ=60)
                    AACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACC                                                                           <  SRR4302547.3936079/94‑1 (MQ=60)
                     ACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCT                                                                          <  SRR4302547.1216689/94‑1 (MQ=60)
                      CAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTG                                                                         >  SRR4302547.2096356/1‑94 (MQ=60)
                      CAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTG                                                                         >  SRR4302547.1475942/1‑94 (MQ=60)
                        ACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTG                                                                       >  SRR4302547.608144/1‑94 (MQ=60)
                         CATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGT                                                                      >  SRR4302547.5369843/1‑94 (MQ=60)
                         CATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGT                                                                      >  SRR4302547.620961/1‑94 (MQ=60)
                         CATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGT                                                                      >  SRR4302547.1577105/1‑94 (MQ=60)
                            CTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACA                                                                   >  SRR4302547.4484605/1‑94 (MQ=60)
                              GCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAG                                                                 >  SRR4302547.4679394/1‑94 (MQ=60)
                                ATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGC                                                               >  SRR4302547.655589/1‑94 (MQ=60)
                                ATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGC                                                               <  SRR4302547.1081628/94‑1 (MQ=60)
                                ATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGC                                                               >  SRR4302547.6003932/1‑94 (MQ=60)
                                 TCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCA                                                              <  SRR4302547.1658152/94‑1 (MQ=60)
                                  CAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAA                                                             <  SRR4302547.41053/94‑1 (MQ=60)
                                  CAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAA                                                             >  SRR4302547.5477517/1‑94 (MQ=60)
                                  CAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAA                                                             <  SRR4302547.41054/94‑1 (MQ=60)
                                  CAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAA                                                             <  SRR4302547.5902449/94‑1 (MQ=60)
                                      ATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCAC                                                         <  SRR4302547.54217/94‑1 (MQ=60)
                                        AAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTG                                                       >  SRR4302547.1159068/1‑94 (MQ=60)
                                        AAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTG                                                       >  SRR4302547.1231164/1‑94 (MQ=60)
                                        AAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTG                                                       <  SRR4302547.2134102/94‑1 (MQ=60)
                                        AAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTG                                                       >  SRR4302547.3714155/1‑94 (MQ=60)
                                        AAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTG                                                       <  SRR4302547.3637070/94‑1 (MQ=60)
                                         AAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGG                                                      <  SRR4302547.714419/94‑1 (MQ=60)
                                          AGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGA                                                     <  SRR4302547.2762923/94‑1 (MQ=60)
                                          AGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGA                                                     <  SRR4302547.6424047/94‑1 (MQ=60)
                                            CAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGC                                                   <  SRR4302547.3927560/94‑1 (MQ=60)
                                            CAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGC                                                   >  SRR4302547.3475282/1‑94 (MQ=60)
                                            CAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGC                                                   >  SRR4302547.1478957/1‑94 (MQ=60)
                                             AAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCT                                                  <  SRR4302547.2156026/94‑1 (MQ=60)
                                             AAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGAACAAGGCAAGCACTGGAGCT                                                  <  SRR4302547.6637209/94‑1 (MQ=60)
                                              AACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTT                                                 >  SRR4302547.1223507/1‑94 (MQ=60)
                                              AACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTT                                                 >  SRR4302547.6341756/1‑94 (MQ=60)
                                                CGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTAT                                               >  SRR4302547.3364120/1‑94 (MQ=60)
                                                 GATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATC                                              <  SRR4302547.2789647/94‑1 (MQ=60)
                                                  ATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCA                                             <  SRR4302547.421514/94‑1 (MQ=60)
                                                  ATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCA                                             <  SRR4302547.4357959/94‑1 (MQ=60)
                                                   TCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAG                                            >  SRR4302547.3618805/1‑94 (MQ=60)
                                                    CAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGT                                           >  SRR4302547.2605113/1‑94 (MQ=60)
                                                    CAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGT                                           >  SRR4302547.5072254/1‑94 (MQ=60)
                                                    CAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGT                                           >  SRR4302547.258890/1‑94 (MQ=60)
                                                     AAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTG                                          <  SRR4302547.3908752/94‑1 (MQ=60)
                                                     AAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTG                                          <  SRR4302547.732618/94‑1 (MQ=60)
                                                      AGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGT                                         >  SRR4302547.2163513/1‑94 (MQ=60)
                                                      AGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGT                                         <  SRR4302547.3329717/94‑1 (MQ=60)
                                                        CGTTAAACCCAATAATTGAAATGCTAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTA                                       >  SRR4302547.521820/1‑94 (MQ=60)
                                                        CGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTA                                       <  SRR4302547.6217654/94‑1 (MQ=60)
                                                         GTTAAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAA                                      <  SRR4302547.4102295/94‑1 (MQ=60)
                                                           TAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATT                                    >  SRR4302547.5073484/1‑94 (MQ=60)
                                                            AAACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTC                                   <  SRR4302547.4179019/94‑1 (MQ=60)
                                                             AACCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCT                                  <  SRR4302547.173964/94‑1 (MQ=60)
                                                               CCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTAT                                >  SRR4302547.2958209/1‑94 (MQ=60)
                                                               CCCAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTAT                                <  SRR4302547.1679466/94‑1 (MQ=60)
                                                                CCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATA                               >  SRR4302547.6110801/1‑94 (MQ=60)
                                                                 CAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATAT                              >  SRR4302547.1153570/1‑94 (MQ=60)
                                                                 CAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATAT                              >  SRR4302547.1749754/1‑94 (MQ=60)
                                                                 CAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATAT                              <  SRR4302547.3681808/94‑1 (MQ=60)
                                                                 CAATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATAT                              >  SRR4302547.2072606/1‑94 (MQ=60)
                                                                  AATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATA                             >  SRR4302547.355477/1‑94 (MQ=60)
                                                                   ATAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAA                            >  SRR4302547.5957289/1‑94 (MQ=60)
                                                                    TAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAAC                           <  SRR4302547.6295734/94‑1 (MQ=60)
                                                                    TAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAAC                           >  SRR4302547.5586002/1‑94 (MQ=60)
                                                                    TAATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAAC                           <  SRR4302547.4073737/94‑1 (MQ=60)
                                                                     AATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACG                          >  SRR4302547.3659662/1‑94 (MQ=60)
                                                                     AATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACG                          <  SRR4302547.5308649/94‑1 (MQ=60)
                                                                     AATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACG                          >  SRR4302547.3753157/1‑94 (MQ=60)
                                                                     AATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACG                          <  SRR4302547.1622261/94‑1 (MQ=60)
                                                                     AATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACG                          <  SRR4302547.73567/94‑1 (MQ=60)
                                                                      ATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGT                         <  SRR4302547.5131260/94‑1 (MQ=60)
                                                                      ATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGT                         <  SRR4302547.5554549/94‑1 (MQ=60)
                                                                      ATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGT                         >  SRR4302547.3327390/1‑94 (MQ=60)
                                                                      ATTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGT                         <  SRR4302547.1918763/94‑1 (MQ=60)
                                                                       TTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTG                        >  SRR4302547.6081790/1‑94 (MQ=60)
                                                                       TTGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTG                        <  SRR4302547.5287533/94‑1 (MQ=60)
                                                                        TGAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGA                       <  SRR4302547.3087899/94‑1 (MQ=60)
                                                                         GAAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGAT                      <  SRR4302547.6307777/94‑1 (MQ=60)
                                                                          AAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATT                     <  SRR4302547.6563106/94‑1 (MQ=60)
                                                                          AAATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATT                     <  SRR4302547.3152739/94‑1 (MQ=60)
                                                                           AATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTC                    <  SRR4302547.2101980/94‑1 (MQ=60)
                                                                           AATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTC                    >  SRR4302547.4956722/1‑94 (MQ=60)
                                                                            ATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCT                   <  SRR4302547.3630772/94‑1 (MQ=60)
                                                                            ATGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAACTTATCAGTGTTAATTCTATATAACGTGATTCT                   >  SRR4302547.799241/1‑94 (MQ=60)
                                                                             TGCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTT                  <  SRR4302547.2705999/94‑1 (MQ=60)
                                                                              GCAAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTA                 >  SRR4302547.4943570/1‑94 (MQ=60)
                                                                               CAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAA                >  SRR4302547.5938608/1‑94 (MQ=60)
                                                                               CAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAA                >  SRR4302547.4796793/1‑94 (MQ=60)
                                                                                AAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAAT               <  SRR4302547.1366545/94‑1 (MQ=60)
                                                                                AAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAAT               <  SRR4302547.2662921/94‑1 (MQ=60)
                                                                                AAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAAT               >  SRR4302547.5566953/1‑94 (MQ=60)
                                                                                AAATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAAT               >  SRR4302547.6733317/1‑94 (MQ=60)
                                                                                  ATAAACGGAAGCACATTGCTCTTGACCAGACCTGTGGACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCCTAATTG             >  SRR4302547.1300016/1‑94 (MQ=60)
                                                                                   TAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGG            <  SRR4302547.1147746/94‑1 (MQ=60)
                                                                                   TAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGG            >  SRR4302547.3976486/1‑94 (MQ=60)
                                                                                   TAAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGG            >  SRR4302547.6734588/1‑94 (MQ=60)
                                                                                    AAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGT           <  SRR4302547.4984197/94‑1 (MQ=60)
                                                                                    AAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGT           >  SRR4302547.175454/1‑94 (MQ=60)
                                                                                    AAACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGT           <  SRR4302547.1011658/94‑1 (MQ=60)
                                                                                     AACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTT          >  SRR4302547.1716145/1‑94 (MQ=60)
                                                                                      ACGGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTT         >  SRR4302547.700083/1‑94 (MQ=60)
                                                                                        GGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTG       <  SRR4302547.1371992/94‑1 (MQ=60)
                                                                                        GGAAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTG       <  SRR4302547.5473487/94‑1 (MQ=60)
                                                                                          AAGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCT     >  SRR4302547.2573092/1‑94 (MQ=60)
                                                                                           AGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTC    >  SRR4302547.5326309/1‑94 (MQ=60)
                                                                                           AGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTC    >  SRR4302547.4156921/1‑94 (MQ=60)
                                                                                           AGCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTAGTTTTGCTC    >  SRR4302547.169760/1‑94 (MQ=60)
                                                                                            GCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTCT   <  SRR4302547.2151232/94‑1 (MQ=60)
                                                                                            GCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTCT   <  SRR4302547.2760675/94‑1 (MQ=60)
                                                                                            GCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTCT   <  SRR4302547.4529758/94‑1 (MQ=60)
                                                                                            GCACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTCA   <  SRR4302547.2705579/94‑1 (MQ=60)
                                                                                             CACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTCTG  <  SRR4302547.3769870/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGGCTAATATCGCCTTTACAAACAACATCTGCATCAAGATAAAGCAAACGATCAAGCGTTAAACCCAATAATTGAAATGCAAATAAACGGAAGTACATTGCTCTTGACCAGACCTGTGTACAAGGCAAGCACTGGAGCTTATCAGTGTTAATTCTATATAACGTGATTCTTAATTGGTTTTGCTCTG  >  NC_000913/3799205‑3799391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑