Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,889,728	C→T	A162V (GCG→GTG)	yidY →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,889,728	0	C	T	91.3%	691.5 / 37.7	229	A162V (GCG→GTG)	yidY	putative transport protein
Reads supporting (aligned to +/- strand): ref base C (8/12); new base T (114/95); total (122/107)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 233 total aligned reads displayed.

TCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAAC  >  NC_000913/3889638‑3889817

tCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGAGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGa                                                                                         >  1:5824028/1‑93 (MQ=255)

 cAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGAt                                                                                        >  1:2059806/1‑93 (MQ=255)

   aCGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATgg                                                                                      >  2:2028747/1‑93 (MQ=255)

      gTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCaa                                                                                   <  1:3471835/93‑1 (MQ=255)

       tATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAat                                                                                  >  1:4487751/1‑93 (MQ=255)

       tATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAat                                                                                  <  1:3376972/93‑1 (MQ=255)

       tATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAat                                                                                  <  1:4629491/93‑1 (MQ=255)

        aTTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAatg                                                                                 >  2:662555/1‑93 (MQ=255)

        aTTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAatg                                                                                 >  1:1495155/1‑93 (MQ=255)

         ttcccTGCATCATTCCGGTGTTAGCGTGAGTGCTCCGACATCTGATTATGCTTAAATTCCCGTGGCACCGTCTGTTCTGTGCGATGGCAatga                                                                                <  1:2586199/90‑1 (MQ=255)

         ttACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAatga                                                                                <  1:1817308/93‑1 (MQ=255)

         ttACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAatga                                                                                <  2:5644038/93‑1 (MQ=255)

         ttACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAatga                                                                                <  2:2841822/93‑1 (MQ=255)

         ttACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAatga                                                                                >  2:1682529/1‑93 (MQ=255)

           aCCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAatgatg                                                                              >  2:5240893/1‑93 (MQ=255)

             cTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATggg                                                                            <  1:4756070/93‑1 (MQ=255)

              tGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGc                                                                           <  1:4441904/93‑1 (MQ=255)

              tGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGc                                                                           >  2:458770/1‑93 (MQ=255)

                 atcatTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATc                                                                        <  2:2501017/93‑1 (MQ=255)

                  tcatTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATcg                                                                       >  1:3104018/1‑93 (MQ=255)

                    atTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATcgcg                                                                     >  1:4858862/1‑93 (MQ=255)

                      tCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGt                                                                   <  1:5412251/93‑1 (MQ=255)

                       ccGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTa                                                                  >  1:3818674/1‑93 (MQ=255)

                        cGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTAc                                                                 >  2:2189932/1‑93 (MQ=255)

                          gtgtTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTg                                                               >  2:4429792/1‑93 (MQ=255)

                          gtgtTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTg                                                               <  1:5209711/93‑1 (MQ=255)

                            gtTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGAt                                                             <  1:3582294/93‑1 (MQ=255)

                             ttAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGAtg                                                            <  2:5071415/93‑1 (MQ=255)

                             ttAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGAtg                                                            >  2:6022285/1‑93 (MQ=255)

                               aGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGAtgtt                                                          >  2:3467667/1‑93 (MQ=255)

                                 cgcCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGAtgttgt                                                        >  2:2447320/1‑93 (MQ=255)

                                  gcCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGAtgttgtt                                                       >  2:5800498/1‑92 (MQ=255)

                                  gcCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTc                                                       >  1:6087654/1‑93 (MQ=255)

                                   ccAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCt                                                      >  2:3545711/1‑93 (MQ=255)

                                     aGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCttt                                                    <  2:1307808/93‑1 (MQ=255)

                                        gCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGtt                                                 <  2:1384366/93‑1 (MQ=255)

                                         cTCGGACATCTGGTTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGttt                                                >  1:2468771/1‑93 (MQ=255)

                                          tCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTa                                               <  1:3920649/93‑1 (MQ=255)

                                          tCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTa                                               <  2:1640900/93‑1 (MQ=255)

                                          tCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTa                                               <  2:2120929/93‑1 (MQ=255)

                                           cGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAt                                              <  2:4111138/93‑1 (MQ=255)

                                            ggACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAtt                                             <  2:4841682/93‑1 (MQ=255)

                                            ggACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAtt                                             >  1:3880721/1‑93 (MQ=255)

                                            ggACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAtt                                             >  1:1147708/1‑93 (MQ=255)

                                             gACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAttt                                            >  1:2480811/1‑93 (MQ=255)

                                              aCATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAtttt                                           <  2:193409/93‑1 (MQ=255)

                                               cATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTa                                          >  1:2135901/1‑93 (MQ=255)

                                                aTCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaa                                         >  1:125214/1‑93 (MQ=255)

                                                aTCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaa                                         >  1:1393423/1‑93 (MQ=255)

                                                aTCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaa                                         <  1:3705089/93‑1 (MQ=255)

                                                aTCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaa                                         >  2:3634175/1‑93 (MQ=255)

                                                aTCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaa                                         >  1:2719476/1‑93 (MQ=255)

                                                aTCTGAGTATGCTTAAATTCCCGGGGCATAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaa                                         <  1:498981/93‑1 (MQ=255)

                                                 tCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaaa                                        <  2:587647/93‑1 (MQ=255)

                                                 tCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaaa                                        >  2:1782665/1‑93 (MQ=255)

                                                 tCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaaa                                        <  2:5563200/93‑1 (MQ=255)

                                                  cTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaaaa                                       <  1:5320136/93‑1 (MQ=255)

                                                  cTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaaaa                                       <  1:5718807/93‑1 (MQ=255)

                                                   tGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaaaaa                                      >  2:1984418/1‑92 (MQ=255)

                                                   tGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAg                                      <  2:1125482/93‑1 (MQ=255)

                                                    gATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGa                                     <  2:5430885/93‑1 (MQ=255)

                                                    gATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGa                                     <  2:783245/93‑1 (MQ=255)

                                                    gATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGa                                     <  1:577456/93‑1 (MQ=255)

                                                     aTTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGaa                                    <  1:1247017/93‑1 (MQ=255)

                                                     aTTATGCTTAAATTCCCGTGGCAGAGACTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGaa                                    >  2:3115424/1‑93 (MQ=255)

                                                      ttATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGaaa                                   <  1:1927862/93‑1 (MQ=255)

                                                       tATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAc                                  <  2:2621422/93‑1 (MQ=255)

                                                       tATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAc                                  <  2:3751390/93‑1 (MQ=255)

                                                        aTGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGGTGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGaaaag                                 >  1:119192/1‑91 (MQ=255)

                                                        aTGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAcg                                 <  1:4093148/93‑1 (MQ=255)

                                                         tGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGGTGTTGTCTTTGTTTATTTTAAAAGAAAcgc                                >  2:3344376/1‑93 (MQ=255)

                                                         tGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAcgc                                <  1:1388757/93‑1 (MQ=255)

                                                         tGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAcgc                                <  2:5785761/93‑1 (MQ=255)

                                                         tGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAcgc                                <  1:5583133/93‑1 (MQ=255)

                                                           cTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACgcgc                              >  2:5325612/1‑93 (MQ=255)

                                                            ttAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGcc                             >  1:2917452/1‑93 (MQ=255)

                                                            ttAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGcc                             >  2:3894349/1‑93 (MQ=255)

                                                             tAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGccc                            >  1:3211574/1‑93 (MQ=255)

                                                              aaaTTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCa                           <  2:2760074/93‑1 (MQ=255)

                                                              aaaTTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCa                           >  2:4685531/1‑93 (MQ=255)

                                                              aaaTTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCa                           <  2:4512651/93‑1 (MQ=255)

                                                               aaTTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAg                          <  2:4543557/93‑1 (MQ=255)

                                                                  tCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCgg                       >  2:1848178/1‑93 (MQ=255)

                                                                  tCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCgg                       >  1:887569/1‑93 (MQ=255)

                                                                   cccGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGc                      <  2:3331146/93‑1 (MQ=255)

                                                                        ggCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCg                 >  1:954954/1‑93 (MQ=255)

                                                                         gCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGc                <  1:6136753/93‑1 (MQ=255)

                                                                          cAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCa               <  1:4146980/93‑1 (MQ=255)

                                                                          cAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCa               >  2:1430030/1‑93 (MQ=255)

                                                                            gagTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGc             <  1:1741899/93‑1 (MQ=255)

                                                                              gTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtt           >  2:3210844/1‑93 (MQ=255)

                                                                              gTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtt           >  2:5143191/1‑93 (MQ=255)

                                                                              gTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtt           >  1:2238606/1‑93 (MQ=255)

                                                                               tCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTc          <  2:5364265/93‑1 (MQ=255)

                                                                                 tGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCgg        <  2:3578720/93‑1 (MQ=255)

                                                                                 tGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCgg        >  1:284022/1‑93 (MQ=255)

                                                                                  gTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGa       >  1:633875/1‑93 (MQ=255)

                                                                                    tCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATa     >  2:2965059/1‑93 (MQ=255)

                                                                                      tGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATaac   >  2:3071862/1‑92 (MQ=255)

                                                                                      tGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATaaa   >  2:4356439/1‑93 (MQ=255)

TCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAAC  >  NC_000913/3889638‑3889817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACG  >  NC_000913/3889636‑3889821

ACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTG                                                                                              <  SRR4302532.4583494/94‑1 (MQ=60)

ACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTG                                                                                              >  SRR4302532.5229347/1‑94 (MQ=60)

ACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTG                                                                                              >  SRR4302532.4229594/1‑94 (MQ=60)

ACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTG                                                                                              <  SRR4302532.3245258/94‑1 (MQ=60)

 CTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGAGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGA                                                                                             >  SRR4302532.6026263/1‑94 (MQ=60)

  ccaaccgtattccctgtacaATTCCGGTGTTGGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGAT                                                                                            <  SRR4302532.3850435/74‑1 (MQ=60)

  TCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGAT                                                                                            >  SRR4302532.2128411/1‑94 (MQ=60)

    AACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGG                                                                                          <  SRR4302532.2979251/94‑1 (MQ=60)

       GGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAA                                                                                       <  SRR4302532.4675983/94‑1 (MQ=60)

       GGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAA                                                                                       <  SRR4302532.2776952/94‑1 (MQ=60)

        TTATTACCTGCTTCATTTCGGTGTTAGCGCCATTGCTCGGACATCTGATTATGCTTTAATTCTCGTGGCAGAGTCTGTTCTGGGCTATGGCAAT                                                                                      >  SRR4302532.5847706/1‑94 (MQ=60)

        GTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAAT                                                                                      <  SRR4302532.3588112/94‑1 (MQ=60)

        GTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAAT                                                                                      >  SRR4302532.4640919/1‑94 (MQ=60)

         TATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATG                                                                                     <  SRR4302532.3489907/94‑1 (MQ=60)

         TATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATG                                                                                     >  SRR4302532.1545593/1‑94 (MQ=60)

         TATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATG                                                                                     <  SRR4302532.2561934/94‑1 (MQ=60)

         TATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATG                                                                                     <  SRR4302532.4787337/94‑1 (MQ=60)

           TTCCCTGCATCATTCCGGTGTTAGCGTGAGTGCTCCGACATCTGATTATGCTTAAATTCCCGTGGCACCGTCTGTTCTGTGCGATGGCAATGAT                                                                                   <  SRR4302532.2672642/94‑1 (MQ=60)

           TTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGAT                                                                                   <  SRR4302532.1877884/94‑1 (MQ=60)

           TTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGAT                                                                                   <  SRR4302532.5406336/94‑1 (MQ=60)

           TTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGAT                                                                                   >  SRR4302532.2130107/1‑94 (MQ=60)

           TTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGAT                                                                                   <  SRR4302532.6313097/94‑1 (MQ=60)

              CCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGG                                                                                >  SRR4302532.4627462/1‑94 (MQ=60)

               CTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGC                                                                               <  SRR4302532.4918764/94‑1 (MQ=60)

                TGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCA                                                                              <  SRR4302532.4817377/94‑1 (MQ=60)

                TGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCA                                                                              <  SRR4302532.4593268/94‑1 (MQ=60)

                  CATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATC                                                                            >  SRR4302532.31216/1‑94 (MQ=60)

                   ATCATTCCGGTGTTAGCGCCAGTGCTCTGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCG                                                                           <  SRR4302532.4131350/94‑1 (MQ=60)

                   ATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCG                                                                           >  SRR4302532.3206954/1‑94 (MQ=60)

                   ATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCG                                                                           >  SRR4302532.2444193/1‑94 (MQ=60)

                     CATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCG                                                                         >  SRR4302532.3609108/1‑94 (MQ=60)

                     CATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCG                                                                         >  SRR4302532.5026022/1‑94 (MQ=60)

                      ATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGG                                                                        >  SRR4302532.3357028/1‑94 (MQ=60)

                        TCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTA                                                                      <  SRR4302532.5599773/94‑1 (MQ=60)

                        TCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTA                                                                      >  SRR4302532.3248857/1‑94 (MQ=60)

                        TCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTA                                                                      >  SRR4302532.3947590/1‑94 (MQ=60)

                          CGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACT                                                                    <  SRR4302532.2931108/94‑1 (MQ=60)

                          CGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACT                                                                    >  SRR4302532.5829804/1‑94 (MQ=60)

                            GTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGA                                                                  <  SRR4302532.5389388/94‑1 (MQ=60)

                             TGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGCGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGCT                                                                 >  SRR4302532.850283/1‑94 (MQ=60)

                             TGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGAT                                                                 >  SRR4302532.4576944/1‑94 (MQ=60)

                              GTTAGCGGCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATG                                                                <  SRR4302532.2220337/94‑1 (MQ=60)

                              GTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATG                                                                <  SRR4302532.3702133/94‑1 (MQ=60)

                              GTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATG                                                                >  SRR4302532.5956304/1‑94 (MQ=60)

                              GTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATG                                                                <  SRR4302532.3989050/94‑1 (MQ=60)

                                TAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTT                                                              <  SRR4302532.161747/94‑1 (MQ=60)

                                GAGCGCCGGTGCGCGGACATCTGCTTAAGCGTAAATTCCCGTGGCAGAGTCTGTGCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTT                                                              <  SRR4302532.4963311/94‑1 (MQ=60)

                                 AGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTG                                                             <  SRR4302532.1684047/94‑1 (MQ=60)

                                   CGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTC                                                           <  SRR4302532.4960780/94‑1 (MQ=60)

                                   CGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTC                                                           >  SRR4302532.6297839/1‑94 (MQ=60)

                                     CCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTT                                                         <  SRR4302532.5624377/94‑1 (MQ=60)

                                     CCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTT                                                         <  SRR4302532.1445859/94‑1 (MQ=60)

                                       AGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTG                                                       >  SRR4302532.1252795/1‑94 (MQ=60)

                                        GTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGT                                                      <  SRR4302532.4606649/94‑1 (MQ=60)

                                          GCTCGGACATCTGGTTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTT                                                    >  SRR4302532.2550961/1‑94 (MQ=60)

                                            TCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAT                                                  <  SRR4302532.4052733/94‑1 (MQ=60)

                                             CGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATT                                                 >  SRR4302532.1186009/1‑94 (MQ=60)

                                             CGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATT                                                 >  SRR4302532.4011714/1‑94 (MQ=60)

                                              GGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTT                                                >  SRR4302532.2563467/1‑94 (MQ=60)

                                                ACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTA                                              >  SRR4302532.2207235/1‑94 (MQ=60)

                                                ACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTA                                              <  SRR4302532.4281487/94‑1 (MQ=60)

                                                ACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTA                                              <  SRR4302532.143383/94‑1 (MQ=60)

                                                 CATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAA                                             <  SRR4302532.5428606/94‑1 (MQ=60)

                                                 CATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAA                                             >  SRR4302532.1440150/1‑94 (MQ=60)

                                                 CATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAA                                             >  SRR4302532.129454/1‑94 (MQ=60)

                                                 CATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAA                                             >  SRR4302532.2810940/1‑94 (MQ=60)

                                                  ATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAA                                            <  SRR4302532.3829468/94‑1 (MQ=60)

                                                  ATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAA                                            >  SRR4302532.3788001/1‑94 (MQ=60)

                                                  ATCTGAGTATGCTTAAATTCCCGGGGCATAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAA                                            <  SRR4302532.516424/94‑1 (MQ=60)

                                                   TCTGATTATGCTTAAATTCCCGTGGCAGCGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTTTTTTAAAA                                           >  SRR4302532.1288777/1‑94 (MQ=60)

                                                    CTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAG                                          <  SRR4302532.5504338/94‑1 (MQ=60)

                                                    CTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAG                                          <  SRR4302532.5918033/94‑1 (MQ=60)

                                                      GATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAA                                        <  SRR4302532.597587/94‑1 (MQ=60)

                                                       ATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAA                                       <  SRR4302532.1288732/94‑1 (MQ=60)

                                                        TTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAC                                      <  SRR4302532.1023695/94‑1 (MQ=60)

                                                        TTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAC                                      <  SRR4302532.1992011/94‑1 (MQ=60)

                                                         TATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGGTGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAAG                                     >  SRR4302532.123270/1‑94 (MQ=60)

                                                         TATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACG                                     <  SRR4302532.2402634/94‑1 (MQ=60)

                                                          ATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGC                                    >  SRR4302532.5057635/1‑94 (MQ=60)

                                                          ATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGC                                    <  SRR4302532.4230554/94‑1 (MQ=60)

                                                          ATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGC                                    <  SRR4302532.2687114/94‑1 (MQ=60)

                                                           TGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCG                                   <  SRR4302532.5776947/94‑1 (MQ=60)

                                                           TGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCG                                   <  SRR4302532.1435306/94‑1 (MQ=60)

                                                           TGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCG                                   <  SRR4302532.1435308/94‑1 (MQ=60)

                                                             CTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCC                                 >  SRR4302532.3014653/1‑94 (MQ=60)

                                                             CTTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTCAAAAGAAACGCGCC                                 <  SRR4302532.5007489/94‑1 (MQ=60)

                                                              TTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCC                                >  SRR4302532.3049308/1‑94 (MQ=60)

                                                              TTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCC                                >  SRR4302532.3318210/1‑94 (MQ=60)

                                                              TTAAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCC                                >  SRR4302532.3854070/1‑94 (MQ=60)

                                                              TGAAATTCCCGTGGCAGAGTCTTTTCTGGGTGATGGCAATGGTGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCC                                <  SRR4302532.1423798/94‑1 (MQ=60)

                                                                AAATTCTCGTGGCAGAGTCTGTCGGCGGTGATGGCAATGATGGGCATCGCTGTACTGATGTTGTCTTTCTTTATTTTAAAACAAACGCGCCCAG                              <  SRR4302532.6224368/94‑1 (MQ=55)

                                                                AAATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAG                              <  SRR4302532.1205320/94‑1 (MQ=60)

                                                                  ATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCG                            <  SRR4302532.5506113/94‑1 (MQ=60)

                                                                  ATTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCG                            <  SRR4302532.932138/94‑1 (MQ=60)

                                                                   TTCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGG                           >  SRR4302532.917907/1‑94 (MQ=60)

                                                                   TCCCCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAACCGCGCCCAGCGG                           <  SRR4302532.2653486/94‑1 (MQ=60)

                                                                      CCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCC                        >  SRR4302532.206022/1‑94 (MQ=60)

                                                                      CCGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCC                        >  SRR4302532.4186618/1‑94 (MQ=60)

                                                                       CGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCC                       >  SRR4302532.6023663/1‑94 (MQ=60)

                                                                       CGTGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCC                       >  SRR4302532.6286496/1‑94 (MQ=60)

                                                                         TGGCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCG                     >  SRR4302532.987580/1‑94 (MQ=60)

                                                                           GCAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCA                   <  SRR4302532.6348447/94‑1 (MQ=60)

                                                                            CAGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAG                  <  SRR4302532.4286115/94‑1 (MQ=60)

                                                                            CAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCAGCCCCCGCAG                  <  SRR4302532.1805179/94‑1 (MQ=60)

                                                                             AGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGC                 >  SRR4302532.19317/1‑94 (MQ=60)

                                                                             AGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGC                 >  SRR4302532.5325017/1‑94 (MQ=60)

                                                                             AGAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGC                 >  SRR4302532.722639/1‑94 (MQ=60)

                                                                              GAGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCT                <  SRR4302532.1800510/94‑1 (MQ=60)

                                                                               AGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTT               >  SRR4302532.2312897/1‑94 (MQ=60)

                                                                               AGTCTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTT               >  SRR4302532.3548249/1‑94 (MQ=60)

                                                                                 TCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCG             <  SRR4302532.20748/94‑1 (MQ=60)

                                                                                  CTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGCTGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGG            >  SRR4302532.4036145/1‑94 (MQ=60)

                                                                                  CTGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGG            >  SRR4302532.293841/1‑94 (MQ=60)

                                                                                   TGTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGA           >  SRR4302532.655992/1‑94 (MQ=60)

                                                                                    GTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGAT          <  SRR4302532.3542681/94‑1 (MQ=60)

                                                                                    GTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGAT          >  SRR4302532.4304637/1‑94 (MQ=60)

                                                                                    GTTCTGGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGAT          >  SRR4302532.6360083/1‑94 (MQ=60)

                                                                                     TTCTGGGTTATGCCATTGATGGGCACCGCGGTCCTGATGTTGTCTTTGTTCATTTTAAAAGAACCGCGCCCAGCGGCCCCCGCAGCTTCGGATA         <  SRR4302532.2466787/94‑1 (MQ=60)

                                                                                         GGGTGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACC     >  SRR4302532.2346202/1‑94 (MQ=60)

                                                                                            TGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACG  >  SRR4302532.4036712/1‑94 (MQ=60)

                                                                                            GGATGCCAATGAGGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACG  <  SRR4302532.3056454/94‑1 (MQ=60)

ACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACG  >  NC_000913/3889636‑3889821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`