Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,945,351	+C	coding (1/600 nt) coding (240/240 nt)	yifDA ← yifDA ←	transcription regulator transcription regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,945,350	1	.	C	99.5%	784.1 / ‑3.8	214	coding (2/600 nt)	yifDA	transcription regulator
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (101/112); total (101/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 232 total aligned reads displayed.

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945258‑3945437

aCAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑Cct                                                                                          >  2:5755356/1‑93 (MQ=255)

  aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑Cct‑cc                                                                                       >  2:846781/1‑92 (MQ=255)

   aGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑Cct‑cct                                                                                      <  2:2914133/93‑3 (MQ=255)

   aGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑Cct‑cct                                                                                      >  2:5030919/1‑91 (MQ=255)

   aGCCTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑Cct‑cct                                                                                      <  2:4866742/93‑3 (MQ=255)

     cGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑Cct‑cctta                                                                                    <  2:169236/93‑5 (MQ=255)

       tCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTTTGCGCCA‑CCTCCTTACg                                                                                   >  2:5496162/1‑93 (MQ=255)

       tCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACg                                                                                   >  1:1127592/1‑93 (MQ=255)

       tCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACg                                                                                   <  2:365822/93‑1 (MQ=255)

        cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACgg                                                                                  <  1:630242/93‑1 (MQ=255)

        cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACgg                                                                                  <  1:3038887/93‑1 (MQ=255)

        cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACgg                                                                                  >  2:623462/1‑93 (MQ=255)

         ccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACggg                                                                                 <  2:1850789/93‑1 (MQ=255)

          cAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGggc                                                                                <  1:5687594/93‑1 (MQ=255)

          cAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGggc                                                                                <  1:1818714/93‑1 (MQ=255)

          cAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGggc                                                                                >  2:403896/1‑93 (MQ=255)

          cAGCCACTGATTAAGCACACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGggc                                                                                >  1:4772026/1‑93 (MQ=255)

            gCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGggcgg                                                                              >  1:5350883/1‑93 (MQ=255)

              cACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGcc                                                                            >  1:1474285/1‑93 (MQ=255)

              cACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGcc                                                                            <  2:3818832/93‑1 (MQ=255)

               ccTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCt                                                                           <  2:790406/92‑1 (MQ=255)

               aCTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCt                                                                           <  1:840624/93‑1 (MQ=255)

                cTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTg                                                                          >  1:962285/1‑93 (MQ=255)

                  taTTAAACATACATTCCCCCAACGAGGCCCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCC‑CCTCCTTACGGGCGGCCTGcc                                                                        <  2:1647460/92‑1 (MQ=255)

                    ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAg                                                                      <  1:1604525/93‑1 (MQ=255)

                    ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAg                                                                      <  2:5354481/93‑1 (MQ=255)

                     tAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAgg                                                                     >  1:188729/1‑93 (MQ=255)

                      aaGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGt                                                                    <  1:5302884/93‑1 (MQ=255)

                         cATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCt                                                                 >  2:2466548/1‑93 (MQ=255)

                         cATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCt                                                                 <  1:4859738/93‑1 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               >  2:1018580/1‑93 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               <  2:3279260/93‑1 (MQ=255)

                            aCATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCAt                                                              <  1:2323093/93‑1 (MQ=255)

                              aTTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGa                                                            >  1:5556141/1‑93 (MQ=255)

                              aTTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGa                                                            <  1:4469810/93‑1 (MQ=255)

                                tCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGc                                                          <  1:1957835/93‑1 (MQ=255)

                                tCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGc                                                          >  1:4983240/1‑93 (MQ=255)

                                  ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        <  1:337174/93‑1 (MQ=255)

                                  ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  1:2552549/1‑93 (MQ=255)

                                  ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  1:3933487/1‑93 (MQ=255)

                                    acaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGttt                                                      <  1:2051429/93‑1 (MQ=255)

                                    acaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGttt                                                      >  2:3405643/1‑93 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGCGCGTTTc                                                     >  2:5680119/1‑93 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  1:55085/93‑1 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     >  1:1663870/1‑93 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  2:5714210/93‑1 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGGGCTCATAAGCGTTTc                                                     >  1:3211315/1‑93 (MQ=255)

                                       aCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTg                                                   >  2:926186/1‑93 (MQ=255)

                                       aCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTg                                                   >  1:3644673/1‑93 (MQ=255)

                                         gAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATCCGCCA‑CCTCCTCACGGGCGGCCTGCCAGGTGCTCATAAGAGTTTCTGCa                                                 >  2:4635225/1‑93 (MQ=255)

                                          aGGCGCGTGCCCCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAt                                                <  2:1556033/93‑1 (MQ=255)

                                               cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGAATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAgga                                           >  2:2274263/1‑92 (MQ=255)

                                                tGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa                                          >  2:645878/1‑93 (MQ=255)

                                                 ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCag                                         <  2:2667245/93‑1 (MQ=255)

                                                  gCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagt                                        >  2:4153369/1‑93 (MQ=255)

                                                  gCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagt                                        >  1:5714115/1‑93 (MQ=255)

                                                   cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  2:4471542/93‑1 (MQ=255)

                                                   cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  1:2687990/93‑1 (MQ=255)

                                                   cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagta                                       <  1:2733885/93‑1 (MQ=255)

                                                     ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     >  1:2123369/1‑93 (MQ=255)

                                                        aTAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtttt                                  <  2:3870862/93‑1 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 <  2:5879023/93‑1 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 >  1:1949052/1‑93 (MQ=255)

                                                          aGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc                                >  2:5402974/1‑93 (MQ=255)

                                                           gAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCa                               <  1:4098348/93‑1 (MQ=255)

                                                           gAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCa                               >  1:1194765/1‑93 (MQ=255)

                                                           gAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCa                               <  2:3902578/93‑1 (MQ=255)

                                                             aaaCTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAcc                             <  2:3025011/93‑1 (MQ=255)

                                                              aaCTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTGATGAGCGTTTCTGGATAAGGTAGCAGTTTTTGACCg                            >  2:4322928/1‑93 (MQ=255)

                                                               aCTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCgg                           >  1:2426293/1‑93 (MQ=255)

                                                                cTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGc                          >  2:2036649/1‑93 (MQ=255)

                                                                cTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGc                          >  1:6037213/1‑93 (MQ=255)

                                                                 tCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCa                         <  1:1019993/93‑1 (MQ=255)

                                                                  cGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAg                        <  1:4212844/93‑1 (MQ=255)

                                                                   gTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc                       <  2:3654179/93‑1 (MQ=255)

                                                                   gTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc                       <  1:4584093/93‑1 (MQ=255)

                                                                   gTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc                       <  2:5219912/93‑1 (MQ=255)

                                                                    ttATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCg                      <  1:1562926/93‑1 (MQ=255)

                                                                     tATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCgg                     >  2:2140417/1‑93 (MQ=255)

                                                                      aTGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    <  1:588344/93‑1 (MQ=255)

                                                                      aTGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    <  1:3978754/93‑1 (MQ=255)

                                                                      aTGTCGTGAGGTATGCGCCA‑CCTCCTTACAGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    <  2:2948378/93‑1 (MQ=255)

                                                                       tGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt                   <  2:2719790/93‑1 (MQ=255)

                                                                         tCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaa                 >  2:467766/1‑93 (MQ=255)

                                                                          cGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaaa                <  2:3227916/93‑1 (MQ=255)

                                                                          cGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaaa                >  2:1156606/1‑93 (MQ=255)

                                                                           gTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaaag               >  1:4544845/1‑92 (MQ=255)

                                                                            tcaGGTATGCGCCACCCC‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACg              <  1:5216173/91‑1 (MQ=255)

                                                                            tGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACg              <  1:2817012/93‑1 (MQ=255)

                                                                            tGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACg              >  2:1401100/1‑93 (MQ=255)

                                                                               ggTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGat           >  1:3854197/1‑93 (MQ=255)

                                                                                gTATGCGCCA‑TCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTGCACCGGCAGCGGTTAAACGGata          <  1:1376151/93‑1 (MQ=255)

                                                                                gTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          <  1:301153/93‑1 (MQ=255)

                                                                                 tATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         <  2:386284/93‑1 (MQ=255)

                                                                                   tGCGCCG‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTg       <  2:471722/93‑1 (MQ=255)

                                                                                   tGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTg       <  1:5100825/93‑1 (MQ=255)

                                                                                    tcgcCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGATCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGt      <  1:5257604/92‑1 (MQ=255)

                                                                                    gcgcCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGt      <  1:403896/93‑1 (MQ=255)

                                                                                    gcgcCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGt      <  1:3318964/93‑1 (MQ=255)

                                                                                     cgcCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGtt     <  1:733379/93‑1 (MQ=255)

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945258‑3945437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  NC_000913/3945259‑3945444

CAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCT‑CC                                                                                              >  SRR4302532.5198867/1‑94 (MQ=60)

 AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCT‑CCT                                                                                             <  SRR4302532.898880/94‑1 (MQ=60)

  AGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCT‑Cctt                                                                                            <  SRR4302532.4396655/94‑4 (MQ=60)

   acgtcccactCACTGATTAAGCATACATTCCCAGAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTA                                                                                            <  SRR4302532.1587437/84‑1 (MQ=60)

    GGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGTCA‑CCTCCTTAC                                                                                           <  SRR4302532.385947/94‑1 (MQ=60)

    CGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTAC                                                                                           <  SRR4302532.168430/94‑1 (MQ=60)

     GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACG                                                                                          >  SRR4302532.1165211/1‑94 (MQ=60)

     GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACG                                                                                          <  SRR4302532.3452047/94‑1 (MQ=60)

      CCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCTCTGGCACCGATAGAAACCTCGTTATGTCGTGAGGTATGCGCCT‑CCTCCTTACGG                                                                                         <  SRR4302532.4350212/94‑1 (MQ=60)

      CCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGG                                                                                         <  SRR4302532.4679085/94‑1 (MQ=60)

       CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGG                                                                                        <  SRR4302532.652253/94‑1 (MQ=60)

       CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGG                                                                                        <  SRR4302532.3139151/94‑1 (MQ=60)

       CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGG                                                                                        >  SRR4302532.4577758/1‑94 (MQ=60)

        CCAGCCACTGATTAAGCACACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGC                                                                                       >  SRR4302532.4936184/1‑94 (MQ=60)

         CAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCG                                                                                      <  SRR4302532.5886098/94‑1 (MQ=60)

         CAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCG                                                                                      <  SRR4302532.1879341/94‑1 (MQ=60)

          AGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGG                                                                                     >  SRR4302532.5536494/1‑94 (MQ=60)

            CCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCC                                                                                   >  SRR4302532.1523957/1‑94 (MQ=60)

            CCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCC                                                                                   >  SRR4302532.3775880/1‑94 (MQ=60)

            CCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCC                                                                                   <  SRR4302532.5608010/94‑1 (MQ=60)

            CCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCC                                                                                   >  SRR4302532.244038/1‑94 (MQ=60)

              ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTG                                                                                 <  SRR4302532.5719983/94‑1 (MQ=60)

              ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTG                                                                                 >  SRR4302532.995123/1‑94 (MQ=60)

              ACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTG                                                                                 <  SRR4302532.869470/94‑1 (MQ=60)

                TGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCC                                                                               <  SRR4302532.1950112/94‑1 (MQ=60)

                 GATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCA                                                                              <  SRR4302532.835578/94‑1 (MQ=60)

                   TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302532.2042454/94‑1 (MQ=60)

                   TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGG                                                                            <  SRR4302532.1658841/94‑1 (MQ=60)

                   TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGG                                                                            >  SRR4302532.195076/1‑94 (MQ=60)

                     AAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTG                                                                          <  SRR4302532.5486154/94‑1 (MQ=60)

                      AGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGC                                                                         >  SRR4302532.662604/1‑94 (MQ=60)

                        CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTC                                                                       <  SRR4302532.5026921/94‑1 (MQ=60)

                         atacaagtgCACAACGAGGCCCTGGCTCCGATAGAAAACTCGTTATGTCGTGAGGTAAGCCCCC‑CCTCCTGACGGGCGGCCTGCCAGGTGCTCA                                                                      <  SRR4302532.1487318/85‑1 (MQ=53)

                         ATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCA                                                                      >  SRR4302532.5478148/1‑94 (MQ=60)

                         ATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCA                                                                      >  SRR4302532.4173848/1‑94 (MQ=60)

                           ACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGTGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATG                                                                    >  SRR4302532.2784733/1‑94 (MQ=60)

                           ACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATG                                                                    <  SRR4302532.2400403/94‑1 (MQ=60)

                           ACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATG                                                                    >  SRR4302532.2905338/1‑94 (MQ=60)

                            CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                   >  SRR4302532.2549554/1‑94 (MQ=60)

                            CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                   >  SRR4302532.5749032/1‑94 (MQ=60)

                             ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  <  SRR4302532.4622212/94‑1 (MQ=60)

                             ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                  >  SRR4302532.3537315/1‑94 (MQ=60)

                              TTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGC                                                                 >  SRR4302532.5154063/1‑94 (MQ=60)

                              TTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGC                                                                 >  SRR4302532.2593996/1‑94 (MQ=60)

                               TCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCG                                                                <  SRR4302532.480256/94‑1 (MQ=60)

                               TCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCG                                                                <  SRR4302532.2022870/94‑1 (MQ=60)

                                GCCACAAGGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTGATGAGCGT                                                               >  SRR4302532.2383355/1‑94 (MQ=60)

                                CCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGT                                                               >  SRR4302532.2637671/1‑94 (MQ=60)

                                CCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGT                                                               >  SRR4302532.4066032/1‑94 (MQ=60)

                                 CCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                              <  SRR4302532.348854/94‑1 (MQ=60)

                                  CACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTT                                                             <  SRR4302532.630796/94‑1 (MQ=60)

                                   ACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTC                                                            <  SRR4302532.2119742/94‑1 (MQ=60)

                                   ACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTC                                                            >  SRR4302532.1720226/1‑94 (MQ=60)

                                   ACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGGGCTCATAAGCGTTTC                                                            >  SRR4302532.3317940/1‑94 (MQ=60)

                                    CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           <  SRR4302532.57053/94‑1 (MQ=60)

                                    CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                           >  SRR4302532.313778/1‑94 (MQ=60)

                                     CACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          <  SRR4302532.1963285/94‑1 (MQ=60)

                                     AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                          >  SRR4302532.3766971/1‑94 (MQ=60)

                                       CGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGTTGCTCATGAGCGTTTCTGCA                                                        <  SRR4302532.473538/94‑1 (MQ=60)

                                        GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                       >  SRR4302532.5819509/1‑94 (MQ=60)

                                         AGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATA                                                      <  SRR4302532.2324163/94‑1 (MQ=60)

                                          GGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAA                                                     >  SRR4302532.5408930/1‑94 (MQ=60)

                                           GCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAG                                                    <  SRR4302532.3412405/94‑1 (MQ=60)

                                           GCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAG                                                    <  SRR4302532.5712405/94‑1 (MQ=60)

                                           GCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAG                                                    <  SRR4302532.4213667/94‑1 (MQ=60)

                                            CGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGG                                                   <  SRR4302532.2932189/94‑1 (MQ=60)

                                               TGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                                <  SRR4302532.5073060/94‑1 (MQ=60)

                                                GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                               >  SRR4302532.5913231/1‑94 (MQ=60)

                                                GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                               <  SRR4302532.4090310/94‑1 (MQ=60)

                                                 GCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTA                                              >  SRR4302532.3307069/1‑94 (MQ=60)

                                                 GCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTA                                              <  SRR4302532.4156785/94‑1 (MQ=60)

                                                  CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             <  SRR4302532.2778274/94‑1 (MQ=60)

                                                  CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             <  SRR4302532.2825779/94‑1 (MQ=60)

                                                  CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                             <  SRR4302532.407049/94‑1 (MQ=60)

                                                   ACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGT                                            >  SRR4302532.2194300/1‑94 (MQ=60)

                                                   ACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGT                                            >  SRR4302532.6032512/1‑94 (MQ=60)

                                                     CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                          <  SRR4302532.1498769/94‑1 (MQ=60)

                                                      GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGCAGTTTT                                         >  SRR4302532.684049/1‑94 (MQ=60)

                                                       ATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTT                                        >  SRR4302532.2013841/1‑94 (MQ=60)

                                                         AGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      >  SRR4302532.1869720/1‑94 (MQ=60)

                                                         AGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      >  SRR4302532.1234700/1‑94 (MQ=60)

                                                         AGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCA                                      <  SRR4302532.3259348/94‑1 (MQ=60)

                                                          GAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAC                                     <  SRR4302532.4235927/94‑1 (MQ=60)

                                                            AAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   >  SRR4302532.1206623/1‑94 (MQ=60)

                                                            AAACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                   >  SRR4302532.1887159/1‑94 (MQ=60)

                                                             AACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGG                                  >  SRR4302532.2506923/1‑94 (MQ=60)

                                                              ACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGC                                 >  SRR4302532.6245717/1‑94 (MQ=60)

                                                              ACTCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGC                                 >  SRR4302532.4948593/1‑94 (MQ=60)

                                                                TCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAG                               <  SRR4302532.5440409/94‑1 (MQ=60)

                                                                TCGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAG                               <  SRR4302532.1054587/94‑1 (MQ=60)

                                                                TCGTTATGTCGTGAGGTATGCCCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAG                               >  SRR4302532.1253434/1‑94 (MQ=60)

                                                                 CGTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGC                              <  SRR4302532.4354251/94‑1 (MQ=60)

                                                                  GTTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                             <  SRR4302532.4740385/94‑1 (MQ=60)

                                                                   TTATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGG                            <  SRR4302532.1615785/94‑1 (MQ=60)

                                                                    TATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGT                           <  SRR4302532.5906078/94‑1 (MQ=60)

                                                                    TATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGT                           <  SRR4302532.5303529/94‑1 (MQ=60)

                                                                     ATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          <  SRR4302532.608866/94‑1 (MQ=60)

                                                                     ATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          <  SRR4302532.4112780/94‑1 (MQ=60)

                                                                     ATGTCGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT                          <  SRR4302532.2022707/94‑1 (MQ=60)

                                                                         CGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAG                      >  SRR4302532.4699980/1‑94 (MQ=60)

                                                                         CGTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                      >  SRR4302532.2180426/1‑94 (MQ=60)

                                                                          GTGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACG                     <  SRR4302532.2741997/94‑1 (MQ=60)

                                                                           TGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    <  SRR4302532.2911298/94‑1 (MQ=60)

                                                                           TGAGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    >  SRR4302532.608292/1‑94 (MQ=60)

                                                                           TCAGGTATGCGCCACCCC‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGG                    <  SRR4302532.5396060/94‑1 (MQ=60)

                                                                             AGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  >  SRR4302532.3984322/1‑94 (MQ=60)

                                                                             AGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  <  SRR4302532.1716634/94‑1 (MQ=60)

                                                                             AGGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                  >  SRR4302532.3437660/1‑94 (MQ=60)

                                                                              GGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 <  SRR4302532.1094818/94‑1 (MQ=60)

                                                                              GGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 >  SRR4302532.2166340/1‑94 (MQ=60)

                                                                              GGTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                 <  SRR4302532.6171386/94‑1 (MQ=60)

                                                                               GTATGCGCCA‑TCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTGCACCGGCAGCGGTTAAACGGATAT                <  SRR4302532.1422269/94‑1 (MQ=60)

                                                                               GTATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT                <  SRR4302532.311583/94‑1 (MQ=60)

                                                                                 ATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTG              <  SRR4302532.2879944/94‑1 (MQ=60)

                                                                                 ATGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTG              >  SRR4302532.1521531/1‑94 (MQ=60)

                                                                                  TGCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGT             <  SRR4302532.5276195/94‑1 (MQ=60)

                                                                                   TCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGATCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT            <  SRR4302532.5438896/94‑1 (MQ=60)

                                                                                   GCGCCG‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT            <  SRR4302532.5502845/94‑1 (MQ=60)

                                                                                   GCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT            <  SRR4302532.418001/94‑1 (MQ=60)

                                                                                   GCGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT            <  SRR4302532.3429628/94‑1 (MQ=60)

                                                                                    CGCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTT           <  SRR4302532.758362/94‑1 (MQ=60)

                                                                                     ccgcgcctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTT           <  SRR4302532.758363/85‑1 (MQ=60)

                                                                                     GCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          >  SRR4302532.3625815/1‑94 (MQ=60)

                                                                                     GCCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTC          >  SRR4302532.49212/1‑94 (MQ=60)

                                                                                      CCA‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         <  SRR4302532.1655581/94‑1 (MQ=60)

                                                                                      CCA‑CCTCCTTACGGGCGGCCTGCCAGGCGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT         >  SRR4302532.5233697/1‑94 (MQ=60)

                                                                                        A‑CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGT       >  SRR4302532.5772657/1‑94 (MQ=60)

                                                                                         ccctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGT       <  SRR4302532.2252066/89‑1 (MQ=60)

                                                                                         ccctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGT       <  SRR4302532.2252065/89‑1 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGGTATTGTTTCTGTGG     >  SRR4302532.3381483/4‑94 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     >  SRR4302532.6350565/4‑94 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     >  SRR4302532.5014567/4‑94 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     >  SRR4302532.863651/4‑94 (MQ=60)

                                                                                           ctcCTTACGGGCGGCATGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG     <  SRR4302532.2378775/91‑1 (MQ=60)

                                                                                            tcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC    <  SRR4302532.4705489/92‑1 (MQ=60)

                                                                                            tcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC    >  SRR4302532.4519276/3‑94 (MQ=60)

                                                                                            tcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGC    >  SRR4302532.3366688/3‑94 (MQ=60)

                                                                                              CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  SRR4302532.819341/1‑94 (MQ=60)

CAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCA‑CCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGG  >  NC_000913/3945259‑3945444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`